CN103055027A - Traditional Chinese medicine composition for inhibiting growth of cancer cells and traditional Chinese medicine extract thereof - Google Patents
Traditional Chinese medicine composition for inhibiting growth of cancer cells and traditional Chinese medicine extract thereof Download PDFInfo
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Abstract
The invention relates to a traditional Chinese medicine composition for inhibiting the growth of cancer cells and a traditional Chinese medicine extract thereof. According to one embodiment, the traditional Chinese medicine composition contains forsythia fruit, peppermint, gardenia, radix scutellariae, bamboo leaves, licorice, rhubarb, mirabilite and atractylodes rhizome. The other embodiments are described in the specification.
Description
The present invention is dividing an application of application number 201010237141.7
Technical field
The invention relates to Chinese medicine composition and the Chinese medicine extract thereof of anticancer growth, refer in particular to the Chinese medicine composition and the Chinese medicine extract thereof that suppress lung cancer cell growth.
Background technology
Cancer is to be positioned at one of ten large causes of the death, and continuous 27 years for occupying the umber one of the ten large causes of the death.The cause cancer cause is mainly: cell becomes unusually, and the autotomize that continues, and forms more abnormal cell, is exactly cancer.
In the general tumor cell, hide a little cancerous cell with stem cell characteristic in the hiding, although this kind cancerous cell quantity is few, similar stem cell can constantly carry out cancerous cell division and differentiation, therefore is referred to as cancer stem cell.Because cancer stem cell has high Drug resistance, the western medicine chemotherapeutic agent is difficult to its poisoning, therefore, often hear many sufferers through chemotherapy, the situation of cancer return in the body, biomedical known standard cancer therapy is felt simply helpless to cancer stem cell at all at present.
Moreover, the at present employed operation of western medicine therapy, radiation cure, chemotherapy, He Ermeng therapy, biological products assay institute etc., usually produce strong side effect for patient body, therefore, if the energy usage comparison is gentle, and can suppress the therapy of cancer stem cell differentiation, carry out treatment of cancer, will be a large Gospel for sufferer.
Now, the common people generally recognize with Chinese medicine the patient are treated, and belong to comparatively gentle Therapeutic Method, and have quite high market acceptance.Therefore, if can develop a kind of Chinese medicine composition, it is behind experiment confirm, but certain anticancer, or stop cancer stem cell to divide, certainly will be able to have suitable help for treatment of cancer.
Summary of the invention
The object of the present invention is to provide a kind of Chinese medicine composition and Chinese medicine extract thereof of anticancer growth, can suppress such as growth of cancer cells such as pulmonary carcinoma.
For achieving the above object, an aspect of the present invention provides a kind of Chinese medicine composition of anticancer growth, comprising: Radix Gentianae (Gentiana scabra Bunge), Radix Scutellariae (Scutellaria baicalensis Georgi), Fructus Gardeniae (Gardeniajasminoides Ellis), Radix Angelicae Sinensis (Angelica sinensis Diels), the Radix Rehmanniae (Rehmannia glutmosa Libosch.f.hueichingensis (Chao et Schih) Hsiao), Caulis Akebiae (Hocquartia manshuriensis (Kom.) Nakai), Radix Bupleuri (Bupleurum falcatum Linne), Semen Plantaginis (Plantago astatica Linne) Cang Intraoperative (Atractylodes lancea De Candolle), Rhizoma Alismatis (Alismaplantago-aquatica L.var.orientale SAM.) and Radix Glycyrrhizae (Glycyrrhiza uralensis Fischer et DC).This Chinese medicine composition is extracted, after also being about to Radix Gentianae, Radix Scutellariae, Fructus Gardeniae, Radix Angelicae Sinensis, the Radix Rehmanniae, Caulis Akebiae, Radix Bupleuri, Semen Plantaginis, grey Intraoperative, Rhizoma Alismatis and Radix Glycyrrhizae mixing, add the extraction heat after-filtration except the extract of medicinal residues gained with water, can reach equally the effect of anticancer growth.
Better, this Radix Gentianae be 1.5 to 6 weight portions, this Radix Scutellariae be 1.5 to 6 weight portions, this Fructus Gardeniae be 1.5 to 6 weight portions, should be classified as 1.5 to 6 weight portions, this Radix Rehmanniae is that 1.5 to 6 weight portions, this Caulis Akebiae are that 1.5 to 6 weight portions, this Radix Bupleuri are that 1.5 to 6 weight portions, this Semen Plantaginis are that 1.5 to 6 weight portions, Gai Cang Intraoperative are that 2 to 8 weight portions, this Rhizoma Alismatis are that 2 to 8 weight portions and this Radix Glycyrrhizae are 2.5 to 10 weight portions.
Better, this Radix Gentianae be 2 to 4 weight portions, this Radix Scutellariae be 2 to 4 weight portions, this Fructus Gardeniae be 2 to 4 weight portions, should be classified as 2 to 4 weight portions, this Radix Rehmanniae is that 2 to 4 weight portions, this Caulis Akebiae are that 2 to 4 weight portions, this Radix Bupleuri are that 2 to 4 weight portions, this Semen Plantaginis are that 2 to 4 weight portions, Gai Cang Intraoperative are that 3 to 6 weight portions, this Rhizoma Alismatis are that 3 to 6 weight portions and this Radix Glycyrrhizae are 4 to 7 weight portions.
Another aspect of the present invention provides a kind of Chinese medicine composition of anticancer growth, comprising: Fructus Forsythiae (Forsythia suspense (Thunb.) Vahl), Herba Menthae (Mentha haploclyx BRIQ.), Fructus Gardeniae (Gardenia jasminoides Ellis), Radix Scutellariae (Scutellaria baicalensis Georgi), Folium Bambusae (Lophatherum gracile Brongniart), Radix Glycyrrhizae (Glycyrrhiza uralensis Fischer et DC), Radix Et Rhizoma Rhei (Rheum palmatum Linne), Mirabilitum (Na
2SO
4) and Cang Intraoperative (Atractylodes lancea De Candolle).This Chinese medicine composition is extracted, after also soon Fructus Forsythiae, Herba Menthae, Fructus Gardeniae, Radix Scutellariae, Folium Bambusae, Radix Glycyrrhizae, Radix Et Rhizoma Rhei, Mirabilitum and grey Intraoperative will mix, add the extraction heat after-filtration except the extract of medicinal residues gained with water, can reach equally the effect of anticancer growth.
Better, this Fructus Forsythiae is that 1.5 to 6 weight portions, this Herba Menthae are that 1.5 to 6 weight portions, this Fructus Gardeniae are that 1.5 to 6 weight portions, this Radix Scutellariae are that 1.5 to 6 weight portions, this Folium Bambusae are that 1.5 to 6 weight portions, this Radix Glycyrrhizae are that 1.5 to 6 weight portions, this Radix Et Rhizoma Rhei are that 0.5 to 2 weight portion, this Mirabilitum are that 0.5 to 2 weight portion and Gai Cang Intraoperative are 2 to 8 weight portions.
Better, this Fructus Forsythiae is that 2 to 4 weight portions, this Herba Menthae are that 2 to 4 weight portions, this Fructus Gardeniae are that 2 to 4 weight portions, this Radix Scutellariae are that 2 to 4 weight portions, this Folium Bambusae are that 2 to 4 weight portions, this Radix Glycyrrhizae are that 2 to 4 weight portions, this Radix Et Rhizoma Rhei are that 0.75 to 1.5 weight portion, this Mirabilitum are that 0.75 to 1.5 weight portion and Gai Cang Intraoperative are 3 to 6 weight portions.
The present invention again another aspect provides the Chinese medicine composition of a kind of anticancer growth, comprising: Radix Angelicae Sinensis (Angelica sinensis Diels), Rhizoma Chuanxiong (Ligusticum chuanxiong Hortorum), Radix Paeoniae Rubra (Paeonia veitchii Lynch.), the Radix Rehmanniae (R.glutinosa Libosch.f.hueichingensis (Chao et Schih.) Hsiao), Radix Glycyrrhizae (Glycyrrhiza uralensis Fischer et DC), Radix Scutellariae (Scutellaria baicalensis Georgi), Cortex Lycii (Lycium chinense Mill.Root), Cortex Moutan (Paeonia suffruticosa Andr.Root) and Cang Intraoperative (Atractylodes lancea De Candolle).This Chinese medicine composition is extracted, after also being about to Radix Angelicae Sinensis, Rhizoma Chuanxiong, Radix Paeoniae Rubra, the Radix Rehmanniae, Radix Glycyrrhizae, Radix Scutellariae, Cortex Lycii, Cortex Moutan and grey Intraoperative mixing, add the extraction heat after-filtration except the extract of medicinal residues gained with water, can reach equally the effect of anticancer growth.
Better, should be classified as 1.5 to 6 weight portions, this Rhizoma Chuanxiong is that 1.5 to 6 weight portions, this Radix Paeoniae Rubra are that 1.5 to 6 weight portions, this Radix Rehmanniae are that 1.5 to 6 weight portions, this Radix Glycyrrhizae are that 1.5 to 6 weight portions, this Radix Scutellariae are that 1.5 to 6 weight portions, this Cortex Lycii are that 2.5 to 10 weight portions, this Cortex Moutan are that 2.5 to 10 weight portions and Gai Cang Intraoperative are 2 to 8 weight portions.
Better, should be classified as 2 to 4 weight portions, this Rhizoma Chuanxiong is that 2 to 4 weight portions, this Radix Paeoniae Rubra are that 2 to 4 weight portions, this Radix Rehmanniae are that 2 to 4 weight portions, this Radix Glycyrrhizae are that 2 to 4 weight portions, this Radix Scutellariae are that 2 to 4 weight portions, this Cortex Lycii are that 4 to 7 weight portions, this Cortex Moutan are that 4 to 7 weight portions and Gai Cang Intraoperative are 2 to 8 weight portions.
The present invention also has an aspect that a kind of Chinese medicine composition of anticancer growth is provided, and comprising: Radix Angelicae Sinensis (Angelica sinensis Diels), Rhizoma Chuanxiong (Ligusticum chuanxiong Hortorum), the Radix Paeoniae Alba (Paeonia lactiflora Pall.), the Radix Rehmanniae (R.glutinosa Libosch.f.hueichingensis (Chao et Schih.) Hsiao) Bai Intraoperative (Atractylodes ovata De Canndolle), Radix Glycyrrhizae (Glycyrrhiza uralensis Fischer et DC), Radix Dipsaci (Dipsacus asper Wall.), the Cortex Eucommiae (Eucommia ulmoides Oliv.), Radix Scutellariae (Scutellaria baicalensis Georgi), Rhizoma Dioscoreae (Dioscorea opposita Thunb.) and Folium Artemisiae Argyi (Artemisia argyi Levl.et Vant.).This Chinese medicine composition is extracted, after also being about to Radix Angelicae Sinensis, Rhizoma Chuanxiong, the Radix Paeoniae Alba, the Radix Rehmanniae, white Intraoperative, Radix Glycyrrhizae, Radix Dipsaci, the Cortex Eucommiae, Radix Scutellariae, Rhizoma Dioscoreae and Folium Artemisiae Argyi mixing, add the extraction heat after-filtration except the extract of medicinal residues gained with water, can reach equally the effect of anticancer growth.
Better, should be classified as 1.5 to 6 weight portions, this Rhizoma Chuanxiong is that 1.5 to 6 weight portions, this Radix Paeoniae Alba are that 1.5 to 6 weight portions, this Radix Rehmanniae are that 1.5 to 6 weight portions, Gai Bai Intraoperative are that 1.5 to 6 weight portions, this Radix Glycyrrhizae are that 1.5 to 6 weight portions, this Radix Dipsaci are that 1.5 to 6 weight portions, this Cortex Eucommiae are that 1.5 to 6 weight portions, this Radix Scutellariae are that 1.5 to 6 weight portions, this Rhizoma Dioscoreae are that 2.5 to 10 weight portions and this Folium Artemisiae Argyi are 2.5 to 10 weight portions.
Better, should be classified as 2 to 4 weight portions, this Rhizoma Chuanxiong is that 2 to 4 weight portions, this Radix Paeoniae Alba are that 2 to 4 weight portions, this Radix Rehmanniae are that 2 to 4 weight portions, Gai Bai Intraoperative are that 2 to 4 weight portions, this Radix Glycyrrhizae are that 2 to 4 weight portions, this Radix Dipsaci are that 2 to 4 weight portions, this Cortex Eucommiae are that 2 to 4 weight portions, this Radix Scutellariae are that 2 to 4 weight portions, this Rhizoma Dioscoreae are that 4 to 7 weight portions and this Folium Artemisiae Argyi are 4 to 7 weight portions.
The other aspect of the present invention provides a kind of Chinese medicine composition of anticancer growth, comprising: beautiful osmanthus (Cinnamomum cassia Presl.), Radix Paeoniae Rubra (Paeonia veitchii Lynch.), Radix Glycyrrhizae (Glycyrrhiza uralensis Fischer et DC), Rhizoma Zingiberis Recens (Zingiber officinale Roscoe), Radix Scutellariae (Scutellaria baicalensis Georgi) and Fructus Jujubae (Ziziphusjujuba Mill.).This Chinese medicine composition is extracted, after also being about to beautiful osmanthus, Radix Paeoniae Rubra, Radix Glycyrrhizae, Rhizoma Zingiberis Recens, Radix Scutellariae and Fructus Jujubae and mixing, add the extraction heat after-filtration except the extract of medicinal residues gained with water, can reach equally the effect of anticancer growth.
Better, this jade osmanthus is that 2.5 to 10 weight portions, this Radix Paeoniae Rubra are that 2.5 to 10 weight portions, this Radix Glycyrrhizae are that 2.5 to 10 weight portions, this Rhizoma Zingiberis Recens are that 2.5 to 10 weight portions, this Radix Scutellariae are that 1.5 to 6 weight portions and this Fructus Jujubae are 2.5 to 10 weight portions.
Better, this jade osmanthus is that 4 to 7 weight portions, this Radix Paeoniae Rubra are that 4 to 7 weight portions, this Radix Glycyrrhizae are that 4 to 7 weight portions, this Rhizoma Zingiberis Recens are that 4 to 7 weight portions, this Radix Scutellariae are that 2 to 4 weight portions and this Fructus Jujubae are 4 to 7 weight portions.
A present invention more other aspect provides the Chinese medicine composition of a kind of anticancer growth, comprising: Rhizoma Cimicifugae (Cimicifuga heracleifoia Kam), the Radix Rehmanniae (R.glutinosa Libosch.F.hueichingensis (Chao et Schich.) Hsiao), Radix Paeoniae Rubra (Paeonia veitchii Lynch.), Cortex Moutan (P.suffruticosa Andr.Root) and Radix Scutellariae (Scutellaria baicalensis Georgi).This Chinese medicine composition extracts, and after also soon Rhizoma Cimicifugae, the Radix Rehmanniae, Radix Paeoniae Rubra, Cortex Moutan and Radix Scutellariae will mix, adds the extraction heat after-filtration except the extract of medicinal residues gained with water, can reach equally the effect of anticancer growth.
Better, this Rhizoma Cimicifugae is that 1 to 4 weight portion, this Radix Rehmanniae are that 7.5 to 30 weight portions, this Radix Paeoniae Rubra are that 5 to 20 weight portions, this Cortex Moutan are that 5 to 20 weight portions and this Radix Scutellariae are 5 to 20 weight portions.
Better, this Rhizoma Cimicifugae is that 1.5 to 3 weight portions, this Radix Rehmanniae are that 10 to 20 weight portions, this Radix Paeoniae Rubra are that 8 to 15 weight portions, this Cortex Moutan are that 8 to 15 weight portions and this Radix Scutellariae are 8 to 15 weight portions.
In addition, in above-mentioned Chinese medicine composition and the Chinese medicine extract thereof, the weight that the heating-up temperature of water and time, water add and the concentrated ratio of water are not particularly limited, and be better as described below: heating system heats water to 90-100 ℃ and lasting 60 minutes to 90 minutes; The weight of water by 5 to 15 times of extraction medical material gross weight; And add extraction heat system water is concentrated into 1/2 to 1/4 of original weight.
Description of drawings
Fig. 1 is the graph of a relation of the survival rate of processing time of the embodiment of the invention 5 and A549 lung carcinoma cell, and after wherein * * * represented to detect (Student ' s t test) and analyze through Xue Shengshi t, the p value was less than 0.001.
After Fig. 2 shows the processing of A549 lung carcinoma cell through the embodiment of the invention 5 different times, the shared ratio of the cell in each cycle, wherein *, * * and * * * represent that respectively the p value is less than 0.05,0.01 and 0.001 through after the Xue Shengshi t detection analysis.
After Fig. 3 showed that the A549 lung carcinoma cell is processed through the embodiment of the invention 5, the cycle was the ratio of the A549 lung carcinoma cell of G0 phase, and after wherein * * * represented to detect (Student ' s t test) and analyze through Xue Shengshi t, the p value was less than 0.001.
The specific embodiment
Below by particular specific embodiment explanation embodiments of the present invention, those skilled in the art can be by content disclosed in the present specification, understanding easily other advantage of the present invention and effect, also can understand following examples and only be explanation usefulness, is not for the restriction scope of the invention.The present invention also can be added by other different specific embodiment, and to implement or to use, the every details in this description also can based on different viewpoints and application, be carried out various modifications and change under not departing from spirit of the present invention.
Embodiment 1
Get it filled material Radix Gentianae 11.25 gram, Radix Scutellariae 11.25 grams, Fructus Gardeniae 11.25 grams, Radix Angelicae Sinensis 11.25 grams, the Radix Rehmanniae 11.25 grams, Caulis Akebiae 11.25 grams, Radix Bupleuri 11.25 grams, Semen Plantaginis 11.25 gram, Cang Intraoperative 15 grams, Rhizoma Alismatis 15 grams and Radix Glycyrrhizae 18.75 grams, after cutting into slices respectively, adding entry 1200 restrains and is heated to more than 90 ℃, continue extraction and become 400 grams after 60 to 90 minutes, the filtering medicinal residues just can obtain extract.
Embodiment 2
Getting it filled, material Fructus Forsythiae 11.25 restrains, Herba Menthae 11.25 restrains, Fructus Gardeniae 11.25 restrains, Radix Scutellariae 11.25 restrains, Folium Bambusae 11.25 restrains, Radix Glycyrrhizae 11.25 restrains, Radix Et Rhizoma Rhei 3.75 restrains, Mirabilitum 3.75 restrains and Cang Intraoperative 15 restrains, after cutting into slices respectively, adding entry 1200 restrains and is heated to more than 90 ℃, continue extraction and become 400 grams after 60 to 90 minutes, the filtering medicinal residues just can obtain extract.
Embodiment 3
Getting it filled, material Radix Angelicae Sinensis 11.25 restrains, Rhizoma Chuanxiong 11.25 restrains, Radix Paeoniae Rubra 11.25 restrains, the Radix Rehmanniae 11.25 restrains, Radix Glycyrrhizae 11.25 restrains, Radix Scutellariae 11.25 restrains, Cortex Lycii 18.75 restrains, Cortex Moutan 18.75 restrains and Cang Intraoperative 15 restrains, after cutting into slices respectively, adding entry 1200 restrains and is heated to more than 90 ℃, continue extraction and become 400 grams after 60 to 90 minutes, the filtering medicinal residues just can obtain extract.
Embodiment 4
Get it filled material Radix Angelicae Sinensis 11.25 gram, Rhizoma Chuanxiong 11.25 grams, the Radix Paeoniae Alba 11.25 grams, the Radix Rehmanniae 11.25 gram, Bai Intraoperative 11.25 grams, Radix Glycyrrhizae 11.25 grams, Radix Dipsaci 11.25 grams, the Cortex Eucommiae 11.25 grams, Radix Scutellariae 11.25 grams, Rhizoma Dioscoreae 18.75 grams and Folium Artemisiae Argyi 18.75 grams, after cutting into slices respectively, adding entry 1200 restrains and is heated to more than 90 ℃, continue extraction and become 400 grams after 60 to 90 minutes, the filtering medicinal residues just can obtain extract.
Embodiment 5
The beautiful osmanthus of the material of getting it filled 18.75 grams, Radix Paeoniae Rubra 18.75 grams, Radix Glycyrrhizae 18.75 grams, Rhizoma Zingiberis Recens 18.75 grams, Radix Scutellariae 11.25 grams and Fructus Jujubae 18.75 grams, after cutting into slices respectively, adding entry 1200 restrains and is heated to more than 90 ℃, continue extraction and become 400 grams after 60 to 90 minutes, the filtering medicinal residues just can obtain extract.
Embodiment 6
Getting it filled, material Rhizoma Cimicifugae 7.5 restrains, the Radix Rehmanniae 56.25 restrains, Radix Paeoniae Rubra 37.5 restrains, Cortex Moutan 37.5 restrains and Radix Scutellariae 37.5 grams, after cutting into slices respectively, add entry 1200 and restrain and be heated to more than 90 ℃, continue extraction and become 400 grams after 60 to 90 minutes, the filtering medicinal residues just can obtain extract.
Test case
Research is pointed out in the cancerous cell, exist a group to represent the cancer stem cell of stem cell characteristic, therefore, if only treat for general cancerous cell, the cancer stem cell that renews in the tumor, because have the characteristic of stem cell, still can be divided into general cancerous cell and form malignant tumor, cause the situation of cancer return after the treatment; Anti-, if treat for cancer stem cell, the cancer stem cell that does not then renew in the tumor, the quantity that can continue to break up and replenish general cancerous cell is just so can suppress tumor.
According to reported in literature, existing scholar is in the A549 lung cancer cell line, isolate the cancer stem cell group with stem cell characteristic, therefore the present invention is in following embodiment, utilize the A549 lung cancer cell line, analyze the extract of Chinese medicine composition of the present invention, on the impact of cell survival rate, and with flow cytometer, detect cell cycle and blocked by Chinese medicine composition of the present invention, also with the staining analysis method, the extract of research Chinese medicine composition of the present invention promotes the effect of cell suicide, again with flow cytometer, Double fluorescence staining method, judge the extract of Chinese medicine composition of the present invention, to the cancerous cell toxic effect.
The test of test case 1 cell survival rate
With embodiment 1 to 6 extract of 10%, 20% and 100% concentration, after 72 hours, measure cell survival rate with mtt assay for the processing of A549 lung carcinoma cell respectively, experimental result is as shown in table 1 below.
Table 1
Survival rate (the %)=average light absorption value of the average light absorption value/matched group of experimental group
Matched group: only process with intermediate water, do not add Chinese medicine and process
Result's demonstration of above-mentioned table 1, along with the consumption of medical composition is higher, the cell survival rate of A549 cell after processing in 72 hours is lower.
Test case 2 is processed time-histories to the impact of cell survival
Result by test case 1, the dosage that partly suppresses that can know embodiment 5 by inference is 35 μ l, uses this partly to suppress dosage and processes the A549 lung carcinoma cell, respectively at 24,48 and 72 hours three time points, detect the survival situation of A549 lung carcinoma cell with mtt assay, its result as shown in Figure 1.
The result of Fig. 1 shows, compared to control group A 549 lung carcinoma cells of processing without embodiment 5, processes 24,48 and 72 hours A549 lung carcinoma cell through embodiment 5, and its survival rate has significant reduction, and along with the time increases, the survival rate difference degree is larger.
Test case 3 cell cycles blocking-up test
(1) PI dyeing
Get the embodiment 5 of half inhibition dosage, 35 μ l, process the A549 lung carcinoma cell after 24,48 and 72 hours, carry out PI dyeing, then with the dna content of flow cytometer detection of lung cancer cell, the result as shown in Figure 2 behind quantitative statistics, wherein, the G0/G1 of transverse axis, S, G2/M represent respectively cell cycle, and the longitudinal axis then represents the shared ratio of each cycle cell.
This test is to observe the A549 lung carcinoma cell under the processing of embodiment 5 (partly suppressing dosage) different time, the distribution scenario of cell cycle.The result of Fig. 2 shows, compared to control group A 549 lung carcinoma cells of processing without embodiment 5, A549 lung carcinoma cell through embodiment 5 processing, no matter be through 24,48 or 72 hours, the cells ratio of its G0/G1 phase all has remarkable rising, and this expression embodiment 5 may cause the A549 lung carcinoma cell to be stuck in the cell cycle of G0/G1.
(2) PI and Ki67 antibody dye altogether
Get half embodiment 5 that suppresses dosage 35 μ l, process the A549 lung carcinoma cell after 72 hours, carry out PI and Ki67 antibody dyes altogether, then detect with flow cytometer, to measure the cells ratio of G0 phase A549 lung carcinoma cell, the result as shown in Figure 3 behind quantitative statistics.
The result of Fig. 3 shows, compared to the A549 lung carcinoma cell of processing without embodiment 5, in the A549 lung carcinoma cell that embodiment 5 processes, the cells ratio that is in the G0 phase has significant rising, this result represents via after embodiment 5 processing, there is more A549 lung carcinoma cell to leave cell cycle, enters the nondividing G0 phase.
By the result of above-mentioned test case, Chinese medicine composition provided by the present invention and Chinese medicine extract thereof can reach the effect that anticancer is grown as can be known, also can allow cancerous cell enter the division lag phase, and then stop cancerous cell to continue division.
Above-described embodiment is only given an example for convenience of description, and the interest field that the present invention advocates is from should with described being as the criterion of claim scope of application, but not only limiting to above-described embodiment.
Claims (2)
1. the Chinese medicine composition of anticancer growth, comprise: Rhizoma Cimicifugae, the Radix Rehmanniae, Radix Paeoniae Rubra, Cortex Moutan and Radix Scutellariae, wherein, this Rhizoma Cimicifugae is that 1 to 4 weight portion, this Radix Rehmanniae are that 7.5 to 30 weight portions, this Radix Paeoniae Rubra are that 5 to 20 weight portions, this Cortex Moutan are that 5 to 20 weight portions and this Radix Scutellariae are 5 to 20 weight portions.
2. the Chinese medicine extract of anticancer growth, comprise: after Rhizoma Cimicifugae, the Radix Rehmanniae, Radix Paeoniae Rubra, Cortex Moutan and Radix Scutellariae are mixed, add the extraction heat after-filtration except the extract of medicinal residues gained with water, wherein, this Rhizoma Cimicifugae is that 1 to 4 weight portion, this Radix Rehmanniae are that 7.5 to 30 weight portions, this Radix Paeoniae Rubra are that 5 to 20 weight portions, this Cortex Moutan are that 5 to 20 weight portions and this Radix Scutellariae are 5 to 20 weight portions.
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| CN103055027B (en) | 2014-05-07 |
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