TWI498119B - Use of extract of chinese medical composition for preparing drug for inhibiting growth of lung cancer cells - Google Patents

Description

Use of water extract of traditional Chinese medicine composition for preparing medicine for inhibiting growth of lung cancer cells

The present invention relates to a drug composition for inhibiting the growth of cancer cells and a traditional Chinese medicine extract thereof, and more particularly to a drug composition for inhibiting the growth of lung cancer cells and a traditional Chinese medicine extract thereof.

The Cancer Department is one of the top ten causes of death and has been at the top of the list of the top ten causes of death for 27 consecutive years. The main cause of cancer is that the cells become abnormal and continue to divide themselves, forming more abnormal cells, which are cancer.

In general, tumor cells have some cancer cells with stem cell characteristics. Although these cancer cells are not many in number, they are similar to stem cells, and can continuously divide and differentiate cancer cells, so they are called cancer stem cells. Because cancer stem cells are highly resistant to drugs, Western medical chemotherapeutic drugs are difficult to kill them. Therefore, many patients who have undergone chemotherapy have been heard, and cancer recurrence in the body. Currently, standard cancer therapies known to biomedicine, cancer stem cells. There is nothing to do.

Moreover, the current surgery, radiotherapy, chemotherapy, hormone therapy, and biologic therapy used in Western medical treatment often have strong side effects on the patient's body. Therefore, if it can be used in a milder manner and can inhibit the differentiation of cancer stem cells, For cancer treatment, it will be a great boon for patients.

Nowadays, people generally recognize that Chinese medicine is used to treat patients. It is a relatively mild treatment method and has a fairly high market acceptance. Therefore, if a Chinese medicine composition can be developed, it can be confirmed by experiments. Inhibiting cancer cells, or preventing cancer stem cells from dividing, is bound to be quite helpful for cancer treatment.

The main object of the present invention is to provide a traditional Chinese medicine composition and a traditional Chinese medicine extract thereof, which can inhibit the growth of cancer cells such as lung cancer.

In order to achieve the above object, an aspect of the present invention provides a drug composition for inhibiting the growth of cancer cells, comprising: Gentiana scabra Bunge, Scutellaria baicalensis Georgi, Gardenia jasminoides Ellis, Angelica ( Angelica sinensis Diels), Rehmannia glutinosa Libosch.f.hueichingensis (Chao et Schih) Hsiao, Hocquartia manshuriensis (Kom.) Nakai, Bupleurum falcatum Linne, Plantago asiatica Linne ), Atractylodes lancea De Candolle, Alisma plantago - aquatica L. var. orientale SAM., and Glycyrrhiza uralensis Fischer et DC. The traditional Chinese medicine composition is extracted, and the gentian, scutellaria, medlar, angelica, shengdi, mutong, beech, psyllium, atractylodes, diarrhea, and licorice are mixed, and then extracted by water and filtered. The extract obtained from the dregs can also achieve the effect of inhibiting the growth of cancer cells.

Preferably, the gentian is 1.5 to 6 parts by weight, the xanthine is 1.5 to 6 parts by weight, the hazelnut is 1.5 to 6 parts by weight, the shovel is 1.5 to 6 parts by weight, and the habitat is 1.5 to 6 parts by weight. Parts by weight, the wood pass is 1.5 to 6 parts by weight, the Bupleurum is 1.5 to 6 parts by weight, and the psyllium is 1.5 to 6 parts by weight, The atractylodes is 2 to 8 parts by weight, the diarrhea is 2 to 8 parts by weight, and the licorice is 2.5 to 10 parts by weight.

More preferably, the gentian is 2 to 4 parts by weight, the sassafras is 2 to 4 parts by weight, the scorpion is 2 to 4 parts by weight, the shovel is 2 to 4 parts by weight, and the habitat is 2 to 4 parts. The parts by weight, the wood pass is 2 to 4 parts by weight, the Bupleurum is 2 to 4 parts by weight, the psyllium is 2 to 4 parts by weight, the atractylodes is 3 to 6 parts by weight, and the diarrhea is 3 to 6 parts by weight and the licorice are 4 to 7 parts by weight.

Another aspect of the present invention provides a pharmaceutical composition for inhibiting the growth of cancer cells, comprising: Forsythia suspense (Thunb.) Vahl, Mentha haploclyx BRIQ., Gardenia jasminoides Ellis, Scutellaria baicalensis Georgi), Lophatherum gracile Brongniart, Glycyrrhiza uralensis Fischer et DC, Rheum palmatum Linne, Na ₂ SO ₄ and Atractylodes lancea De Candolle. The extract of the traditional Chinese medicine is extracted, and the extract obtained by the combination of forsythia, peppermint, medlar, scutellaria, bamboo, licorice, rhubarb, saponin and atractylodes, which is heated and extracted by water and filtered to remove the dregs, can also be obtained. The effect of inhibiting the growth of cancer cells.

Preferably, the forsythia is 1.5 to 6 parts by weight, the mint is 1.5 to 6 parts by weight, the hazelnut is 1.5 to 6 parts by weight, the xanthine is 1.5 to 6 parts by weight, and the bamboo leaves are 1.5 to 6 parts by weight. The licorice is 1.5 to 6 parts by weight, the rhubarb is 0.5 to 2 parts by weight, the plaque is 0.5 to 2 parts by weight, and the atractylodes is 2 to 8 parts by weight.

More preferably, the forsythia is 2 to 4 parts by weight, the mint is 2 to 4 parts by weight, the hazelnut is 2 to 4 parts by weight, the xanthine is 2 to 4 parts by weight, and the bamboo leaves are 2 to 4 parts by weight. The licorice is 2 to 4 parts by weight, the rhubarb is 0.75 to 1.5 parts by weight, the plaque is 0.75 to 1.5 parts by weight, and the atractylodes is 3 to 6 parts by weight.

Yet another aspect of the present invention provides a composition for inhibiting the growth of cancer medicine, comprising: angelica (Angelica sinensis Diels), Chuanxiong (Ligusticum chuanxiong Hortorum), red peony (Paeonia veitchii Lynch.), Habitat (R.glutinosa Libosch. F.hueichingensis (Chao et Schih.) Hsiao), Glycyrrhiza uralensis Fischer et DC, Scutellaria baicalensis Georgi, Lycium chinense Mill.Root, Paeonia suffruticosa Andr.Root, and Atractylodes ( Atractylodes lancea De Candolle). The Chinese medicinal composition is extracted, and the extract obtained by extracting the dregs by heating and extracting with water, and then extracting the extract of the dregs, is also the same as that of Angelica, Chuanxiong, Radix Paeoniae Alba, Radix Scutellariae, Radix Paeoniae Alba, Radix Scutellariae, Radix Scutellariae, Radix Scutellariae, and Atractylodes Achieve the effect of inhibiting the growth of cancer cells.

Preferably, the angelica is 1.5 to 6 parts by weight, the hawthorn is 1.5 to 6 parts by weight, the erythroside is 1.5 to 6 parts by weight, the raw land is 1.5 to 6 parts by weight, and the licorice is 1.5 to 6 parts by weight. The xanthine is 1.5 to 6 parts by weight, the bone skin is 2.5 to 10 parts by weight, the paeonol is 2.5 to 10 parts by weight, and the atractylodes is 2 to 8 parts by weight.

More preferably, the angelica is 2 to 4 parts by weight, the chuanxiong is 2 to 4 parts by weight, the erythroside is 2 to 4 parts by weight, the raw land is 2 to 4 parts by weight, and the licorice is 2 to 4 parts by weight. The scutellaria is 2 to 4 parts by weight, and the bone skin is 4 To 7 parts by weight, the paeonol is 4 to 7 parts by weight and the atractylodes is 2 to 8 parts by weight.

The present invention is also one aspect there is provided a medicine composition of growth inhibition of cancer cells, comprising: (. Paeonia lactiflora Pall) Angelica (Angelica sinensis Diels), Chuanxiong (Ligusticum chuanxiong Hortorum), white peony root, habitat (R.glutinosa Libosch.f .hueichingensis (Chao et Schih.) Hsiao ), Atractylodes (Atractylodes ovata De Canndolle), licorice (Glycyrrhiza uralensis Fischer et DC), teasel (Dipsacus asper Wall.), Eucommia (Eucommia ulmoides Oliv.), skullcap (Scutellaria baicalensis Georgi ), yam ( Dioscorea opposita Thunb.) and wormwood ( Artemisia argyi Levl.et Vant.). The Chinese medicinal composition is extracted, and the extract obtained by extracting the slag after heating and extracting with water, and extracting the slag from the genus Angelica, Chuanxiong, Baiji, Shengdi, Baizhu, Licorice, Dioscorea, Eucommia, Astragalus, Yam, and wormwood It can also achieve the effect of inhibiting the growth of cancer cells.

Preferably, the angelica is 1.5 to 6 parts by weight, the hawthorn is 1.5 to 6 parts by weight, the chalk is 1.5 to 6 parts by weight, the habitat is 1.5 to 6 parts by weight, and the atractylodes is 1.5 to 6 parts by weight. The licorice is 1.5 to 6 parts by weight, the continuation is 1.5 to 6 parts by weight, the eucommia is 1.5 to 6 parts by weight, the xanthine is 1.5 to 6 parts by weight, the yam is 2.5 to 10 parts by weight, and the wormwood It is from 2.5 to 10 parts by weight.

More preferably, the angelica is 2 to 4 parts by weight, the hawthorn is 2 to 4 parts by weight, the chalk is 2 to 4 parts by weight, the habitat is 2 to 4 parts by weight, and the atractylodes is 2 to 4 parts by weight. The licorice is 2 to 4 parts by weight, and the continuation is 2 to 4 parts by weight, the dude is 2 to 4 parts by weight, the xanthine is 2 to 4 parts by weight, the yam is 4 to 7 parts by weight, and the wormwood is 4 to 7 parts by weight.

Another aspect of the present invention provides a pharmaceutical composition for inhibiting the growth of cancer cells, comprising: Cinnamomum cassia Presl., Paeonia veitchii Lynch., Glycyrrhiza uralensis Fischer et DC, Ginger ( Zingiber) Officinale Roscoe), Scutellaria baicalensis Georgi and Ziziphus jujuba Mill. The Chinese medicinal composition is extracted, and the extracts obtained by mixing the scent of osmanthus, red peony, licorice, ginger, scutellaria, and red dates are heated and extracted with water, and the extract obtained by filtering the slag is filtered to achieve the same effect of inhibiting the growth of cancer cells. .

Preferably, the osmanthus is 2.5 to 10 parts by weight, the erythroside is 2.5 to 10 parts by weight, the licorice is 2.5 to 10 parts by weight, the ginger is 2.5 to 10 parts by weight, and the scutellaria is 1.5 to 6 parts by weight. The parts by weight and the red dates are from 2.5 to 10 parts by weight.

More preferably, the osmanthus is 4 to 7 parts by weight, the erythritol is 4 to 7 parts by weight, the licorice is 4 to 7 parts by weight, the ginger is 4 to 7 parts by weight, and the jaundice is 2 to 4 parts. The parts by weight and the red dates are 4 to 7 parts by weight.

Still another aspect of the present invention provides a pharmaceutical composition for inhibiting the growth of cancer cells, comprising: Cimicifuga heracleifolia Kam, R. glutinosa Libosch. F. hueichingensis (Chao et Schich.) Hsiao, and red peony ( Paeonia veitchii Lynch.), P. suffruticosa Andr. Root, and Scutellaria baicalensis Georgi. The composition of the traditional Chinese medicine is extracted, and the extract obtained by mixing the cohosh, the raw land, the red peony, the paeonol and the astragalus, and then extracting the residue by heating with water, can also achieve the effect of inhibiting the growth of cancer cells.

Preferably, the cohosh is 1 to 4 parts by weight, the raw land is 7.5 to 30 parts by weight, the erythroside is 5 to 20 parts by weight, the paeonol is 5 to 20 parts by weight, and the scutellaria is 5 to 20 parts. Parts by weight.

More preferably, the cohosh is 1.5 to 3 parts by weight, the raw land is 10 to 20 parts by weight, the erythritol is 8 to 15 parts by weight, the paeonol is 8 to 15 parts by weight, and the jaundice is 8 to 15 Parts by weight.

Further, in the above-mentioned Chinese medicine composition and its Chinese medicine extract, the heating temperature and time of water, the weight of water addition, and the concentration ratio of water are not particularly limited, and it is preferably as follows: the heating system heats the water to 90-100 ° C and It lasts from 60 minutes to 90 minutes; the weight of water is 5 to 15 times the total weight of the extracted herbs; and the heated extraction concentrates the water to 1/2 to 1/4 of the original weight.

The embodiments of the present invention are described in the following by way of specific examples. Those skilled in the art can readily understand the advantages and advantages of the present invention as disclosed in the present disclosure. It is not intended to limit the scope of the invention. The present invention may be embodied or applied in various other specific embodiments, and various modifications and changes can be made without departing from the spirit and scope of the invention.

Example 1

Take the medicinal material gentian grass 11.5g, astragalus 11.25g, medlar 11.25g, angelica 11.25g, raw land 11.25g, Mutong 11.25g, Beichaihu 11.25 克, psyllium 11.25 grams, 15 grams of Atractylodes, 15 grams of Alisma and 18.75 grams of licorice, respectively, after slicing, add 1200 grams of water and heated to above 90 ° C, continuous extraction for 60 to 90 minutes, then into 400 grams, filtered The extract can be obtained from the dregs.

Example 2

Take the medicine Forsythia 11.25g, peppermint 11.25g, medlar 11.25g, Astragalus 11.25g, bamboo leaf 11.25g, licorice 11.25g, rhubarb 3.75g, Parker nitrate 3.75g and Atractylodes 15g, respectively, after slicing, add 1200g of water And heating to above 90 ° C, continuous extraction for 60 to 90 minutes, 400 grams, filter off the dregs to obtain the extract.

Example 3

Take the medicine of 11.5 grams of Angelica, 11.25 grams of Chuanxiong, 11.25 grams of Radix Paeoniae, 11.25 grams of raw land, 11.25 grams of licorice, 11.25 grams of Astragalus, 18.75 grams of earth bone, 18.75 grams of Danpi and 15 grams of Atractylodes, respectively, after sectioning, add water 1200 The gram is heated to above 90 ° C, and after continuous extraction for 60 to 90 minutes, it is made into 400 g, and the extract is filtered to obtain an extract.

Example 4

Take the medicine of 11.5 grams of Angelica, 11.25 grams of Chuanxiong, 11.25 grams of Radix Paeoniae, 11.25 grams of raw land, 11.25 grams of Atractylodes, 11.25 grams of licorice, 11.25 grams of licorice, 11.25 grams of Eucommia, 11.25 grams of Eucommia, 11.25 grams of Astragalus, 18.75 grams of Chinese yam, and 18.75 grams of wormwood, respectively. After slicing, 1200 g of water was added and heated to 90 ° C or higher, and after continuous extraction for 60 to 90 minutes, it was made into 400 g, and the extract was filtered off to obtain an extract.

Example 5

Take the medicinal material Yugui 18.75g, red peony 18.75g, licorice 18.75g, ginger 18.75g, astragalus 11.25g and red dates 18.75g, respectively, after slicing, add 1200g of water and heat to above 90 °C, continue to extract 60 to 90 After 400 minutes, the extract was obtained by filtering out the dregs.

Example 6

Take 7.5 grams of medicinal herbs, 56.25 grams of raw land, 37.5 grams of red peony, 37.5 grams of danshen and 37.5 grams of astragalus. After slicing, add 1200 grams of water and heat to above 90 °C. Continue to extract for 60 to 90 minutes and then into 400. Gram, the extract is obtained by filtering out the dregs.

Test case

Studies have shown that there are a group of cancer stem cells that display the characteristics of stem cells in cancer cells. Therefore, if only cancer cells are treated for general cancer cells, the cancer stem cells that persist in the tumor can differentiate into general cancer cells because of the characteristics of stem cells. Malignant tumors cause cancer recurrence after treatment; conversely, if cancer stem cells are treated, there are no cancer stem cells that persist in the tumor, which can continue to differentiate and supplement the number of general cancer cells, thus inhibiting the tumor.

According to the literature, it has been reported that a cancer stem cell population having stem cell characteristics has been isolated from A549 lung cancer cell lines. Therefore, in the following examples, the A549 lung cancer cell line was used to analyze the extract of the traditional Chinese medicine composition of the present invention. The effect on cell viability is measured by flow cytometry, and whether the cell cycle is blocked by the composition of the traditional Chinese medicine of the present invention, and the extract of the traditional Chinese medicine composition of the present invention is also studied by a staining analysis method to promote the self-suppressing effect of the cell, and then The extract of the traditional Chinese medicine composition of the present invention was judged by flow cytometry and double fluorescent staining to have a poisoning effect on cancer cells.

Test Example 1 Cell viability test

The extracts of Examples 1 to 6 at 10%, 20% and 100% concentrations were treated for A549 lung cancer cells for 72 hours, and the cell viability was measured by MTT assay. The results are shown in Table 1 below.

The results of Table 1 above show that the higher the amount of the pharmaceutical composition, the lower the cell viability of A549 cells after 72 hours of treatment.

Test Example 2 Effect of treatment time on cell survival

From the results of Test Example 1, it can be inferred that the amount of the semi-inhibitor of Example 5 was 35 μl, and the A549 lung cancer cells were treated with the half inhibitor amount, and A549 lung cancer cells were detected by MTT method at three time points of 24, 48 and 72 hours, respectively. The survival situation, the results are shown in Figure 1.

The results in Figure 1 show that the survival rate of A549 lung cancer cells treated with Example 5 for 24, 48 and 72 hours was significantly lower than that of the control A549 lung cancer cells not treated in Example 5, and with time. Increase, the greater the difference in survival rate.

Test Example 3 Cell Cycle Blocking Test (1) PI staining

Example 5, in which the amount of the semi-inhibitor was 35 μl, was treated with PI staining for 24, 48 and 72 hours after treatment of A549 lung cancer cells, and then the DNA content of the lung cancer cells was detected by flow cytometry. The results are shown in Figure 2 after quantitative analysis. Wherein, the horizontal axis G0/G1, S, G2/M represent the cell cycle, and the vertical axis represents the ratio of cells in each cycle.

This test was to observe the distribution of cell cycle in A549 lung cancer cells treated with Example 5 (semi-inhibitor amount) at different times. The results of Figure 2 show that the A549 lung cancer cells treated with Example 5, whether after 24, 48 or 72 hours, had a G0/G1 phase cell ratio compared to the control A549 lung cancer cells not treated in Example 5. There was a significant increase, which indicates that Example 5 may cause A549 lung cancer cells to arrest in the G0/G1 cell cycle.

(2) PI and Ki67 antibody co-staining

Example 5, in which the amount of the semi-inhibitor was 35 μl, was treated with A549 lung cancer cells for 72 hours, and then co-stained with PI and Ki67 antibodies, and then detected by flow cytometry to measure the cell ratio of G0 phase A549 lung cancer cells, after quantitative analysis. The result is shown in Figure 3.

The results of Fig. 3 show that the A549 lung cancer cells treated in Example 5 were in the G0 phase compared to the A549 lung cancer cells not treated in Example 5. There was a significant increase in the cell ratio, and this result indicates that after treatment with Example 5, more A549 lung cancer cells left the cell cycle and entered the G0 phase of non-dividing.

From the results of the above test examples, it can be seen that the Chinese medicine composition and the traditional Chinese medicine extract thereof provided by the invention can achieve the effect of inhibiting the growth of cancer cells, and can also allow cancer cells to enter a stage of division and stagnation, thereby preventing the cancer cells from continuing to divide.

The above-mentioned embodiments are merely examples for convenience of description, and the scope of the claims is intended to be limited to the above embodiments.

Figure 1 is a graph showing the relationship between the treatment time of Example 5 of the present invention and the survival rate of A549 lung cancer cells, wherein *** indicates that the p value is less than 0.001 after analysis by Student's t test.

2 shows the ratio of cells in each cycle after A549 lung cancer cells were treated at different times in Example 5 of the present invention, wherein *, **, and *** respectively indicate that the p value is less than 0.05 after the Student's t-test analysis. 0.01 and 0.001.

Figure 3 shows the ratio of A549 lung cancer cells in the G0 phase of A549 lung cancer cells treated with Example 5 of the present invention, wherein *** indicates that the p value is less than 0.001 after Student's t test.

Claims (4)

The use of an aqueous extract of a traditional Chinese medicine composition for preparing a medicament for inhibiting the growth of lung cancer cells, the Chinese medicine composition comprising: mixing forsythia, mint, medlar, scutellaria, bamboo leaves, licorice, rhubarb, saponin and atractylodes The extract obtained by filtering the dregs after heating with water; wherein the forsythia is 1.5 to 6 parts by weight, the mint is 1.5 to 6 parts by weight, the hazelnut is 1.5 to 6 parts by weight, and the xanthine is 1.5 to 6 parts by weight. The bamboo leaves are 1.5 to 6 parts by weight, the licorice is 1.5 to 6 parts by weight, the rhubarb is 0.5 to 2 parts by weight, the plaque is 0.5 to 2 parts by weight, and the atractylodes is 2 to 8 parts by weight. The use of claim 1, wherein the heating heats the water to 90-100 ° C for 60 minutes to 90 minutes. The use of claim 1, wherein the water has a weight of 5 to 15 times the total weight of the extracted medicinal material. The use of claim 1, wherein the heated extraction concentrates the water to 1/2 to 1/4 of the original weight.