CN105613612A - Agent for disinfecting intensive care unit and preparation method - Google Patents
Agent for disinfecting intensive care unit and preparation method Download PDFInfo
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- CN105613612A CN105613612A CN201511001763.9A CN201511001763A CN105613612A CN 105613612 A CN105613612 A CN 105613612A CN 201511001763 A CN201511001763 A CN 201511001763A CN 105613612 A CN105613612 A CN 105613612A
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Classifications
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Abstract
The invention relates to an agent for disinfecting an intensive care unit and a preparation method. The prepared agent is prepared from the effective ingredients including Chinese wedelia herb or roots, the root of straight ladybell, square-stems, herba clerodendranthi, tender catchweed bedstraw herb, common andrographis herb, rhizomes of chlorophytum comosum, leaves of Ligustrum sinense Lour., folium eucalypti, ternate grape fern herb, clerodendrum fortunatum roots, herb of klein cinquefoil, burdock roots, cudweed herb, locust tree branches and paniculate swallowwort roots. The medicine materials selected in the agent are suitable in compatibility and are in proper proportion, the advantages of being high in bactericidal ability, high in acting speed, high in stability, low in price and the like are achieved, the agent can be widely applied to disinfecting the skin of personnel, instruments and the interior in the intensive care unit, and is free of simulation to the skin of the human body, and no residue exists after the agent is used; the agent can be easily dissolved in water, free of corrosivity, safe and free of toxic and side effects, the work efficiency of medical staff can be improved, and the agent is an ideal agent worthy of popularization.
Description
Technical field
The present invention relates to the field of Chinese medicines, in particular to a kind of medicament for disinfection of intensive care unit and preparation method.
Background technology
Different from common section office wards, intensive care unit (ICU) mainly accepts various critical illness patient for medical treatment, because conditions of patients is critical, there is serious organismic internal environment disorderly, immune dysfunction, and basic chronic diseases is more, very easily produces various complication, patient has use microbiotic history more before entering ICU, therefore patient's ICU ward infection incidence is obviously higher than public ward. Meanwhile, for medical personnel, work under this kind of Working environment and the probability that is subject to courses of infection increases.
It is reported, in ICU, principal causative bacterium is streptococcus aureus and intestinal bacteria. Research for pathogenic bacterium for many years shows, use western medicine antibiotics class to carry out sterilization and often produce resistance, along with people are more and more higher for pursuit green, naturally demand, it is good and to the sterilization for intensive care unit of the medicament of human body nonirritant that we need to find a kind of disinfection effect.
Summary of the invention
The present invention provides a kind of medicament for disinfection of intensive care unit and preparation method; Selected by medicament of the present invention, medicinal material compatibility is suitable, all medicine phases and there is the advantages such as sterilizing ability is strong, speed of action is fast, stability is strong, cheap, intensive care unit personnel's skin, apparatus and indoor sterilization can be widely used in, human skin is non-stimulated, by rear noresidue, soluble in water, non-corrosiveness, safe without toxic side effect, the working efficiency of medical personnel can be improved, it is a kind of desirable medicament being worthy to be popularized.
For achieving the above object, the technical scheme of the present invention is implemented as follows:
For medicament and the preparation method of disinfection of intensive care unit, make described medicament effective constituent raw material composition and weight part number be:
Herbia Wedeliae 60-90 part the root of straight ladybell 58-88 part Spica Prunellae 58-88 part nauclea officinalis 55-83 part
Tender Catchweed Bedstraw Herb 53-81 part Herba Andrographis 53-81 part bracketplant 48-78 part ligustrum sinense 48-78 part
Folium eucalypti 43-71 part Ternate Grape Fern Herb 43-71 part Root of Redcalyx Glorybower 41-69 part snake is containing 38-65 part
Root of Great Burdock 38-65 part Gnaphalium affine 33-58 part Chinese scholartree branch 33-58 part Radix Cynanchi Paniculati 23-43 part.
Make described medicament effective constituent raw material composition and weight part number be:
Herbia Wedeliae 81 parts of the root of straight ladybell 79 portions of Spica Prunellaes, 79 portions of nauclea officinalis 75 parts
Tender Catchweed Bedstraw Herb 73 parts of Herba Andrographis 73 portions of bracketplant, 68 parts of ligustrum sinenses 68 parts
Folium eucalypti 61 portions of Ternate Grape Fern Herbs 61 parts of Root of Redcalyx Glorybower, 59 portions of snakes are containing 56 parts
Root of Great Burdock 56 portions of Gnaphalium affines 48 parts of Chinese scholartree branches, 48 parts of Radix Cynanchi Paniculatis 36 parts.
Make described medicament effective constituent raw material composition and weight part number be:
Herbia Wedeliae 74 parts of the root of straight ladybell 72 portions of Spica Prunellaes, 72 portions of nauclea officinalis 67 parts
Tender Catchweed Bedstraw Herb 65 parts of Herba Andrographis 65 portions of bracketplant, 62 parts of ligustrum sinenses 62 parts
Folium eucalypti 55 portions of Ternate Grape Fern Herbs 55 parts of Root of Redcalyx Glorybower, 53 portions of snakes are containing 50 parts
Root of Great Burdock 50 portions of Gnaphalium affines 43 parts of Chinese scholartree branches, 43 parts of Radix Cynanchi Paniculatis 32 parts.
Make described medicament effective constituent raw material composition and weight part number be:
Herbia Wedeliae 67 parts of the root of straight ladybell 65 portions of Spica Prunellaes, 65 portions of nauclea officinalis 61 parts
Tender Catchweed Bedstraw Herb 59 parts of Herba Andrographis 59 portions of bracketplant, 56 parts of ligustrum sinenses 56 parts
Folium eucalypti 49 portions of Ternate Grape Fern Herbs 49 parts of Root of Redcalyx Glorybower, 47 portions of snakes are containing 44 parts
Root of Great Burdock 44 portions of Gnaphalium affines 38 parts of Chinese scholartree branches, 38 parts of Radix Cynanchi Paniculatis 27 parts.
For the medicament of disinfection of intensive care unit and the preparation method of preparation method, comprise following processing step:
(1) by Herbia Wedeliae, Spica Prunellae, nauclea officinalis, Herba Andrographis, bracketplant, ligustrum sinense, folium eucalypti, Ternate Grape Fern Herb, Root of Redcalyx Glorybower and snake containing putting into container, add 10 times amount distilled water immersions after 4 hours, heated and boiled 4 hours, extract; Second time adds 8 times amount distilled water heated and boiled 3 hours, extracts; Third time adds 6 times amount distilled water heated and boiled 2 hours, extracts; Merge three gained extracting solutions, filter, obtain filtrate for subsequent use;
(2), after the dilution of honey certain amount of boiling water will be refined, add in Root of Upright Ladybell sheet and mix thoroughly, vexed, put in pot, with slow fire heating, fry to yellowish-orange, tack-free is degree, and taking-up cools, and grinds into powder, must be 100 object fine powders,
For subsequent use;
(3) Tender Catchweed Bedstraw Herb being smash juice, filter impurity, stays juice, for subsequent use;
(4) after Root of Great Burdock, Gnaphalium affine, Chinese scholartree branch and Radix Cynanchi Paniculati being ground into powder, immersing volumetric concentration is 90% spirituous solution, leaves standstill 4 hours, takes out supernatant liquid, and the medicinal extract precipitated is for subsequent use;
(5) by filtrate made for above-mentioned steps (1)-(4), fine powder, juice and medicinal extract mixing, and add the distilled water of 10-12 times amount, and after stirring and dissolving is even, heated and boiled 1 hour, put less than 60 DEG C, leave standstill 1 hour, filter, obtain filtrate, seal up for safekeeping filling for filtrate, 121 DEG C of pressure sterilizings 30 minutes, packaging, is the preparation method of the described medicament for disinfection of intensive care unit.
The property of medicine of the present invention's Chinese medicine used is as follows:
Herbia Wedeliae: mildly bitter flavor, sweet, cool in nature. Clearing heat and detoxicating, cool the blood dissipate blood stasis. Main cold, fever, pharyngolaryngitis, tonsillitis, rib adenositis, diphtheria, Whooping cough, trachitis, pneumonia, pulmonary tuberculosis is spat blood, nosebleed epistaxis, hematuria, infectious hepatitis, dysentery, hemorrhoid, the swollen poison in malignant boil madhouse. " Fujian herbal medicine " carries: " clearing heat and detoxicating. Control diphtheria, Whooping cough, tuberculosis fever and cough, dysentery, high liver fire, dysphoria with smothery sensation egersis, swelling and pain in the throat, gingivitis. " modern medicine study confirmation, this product decoction 1:128 is to diphtheria corynebacterium, and beta streptococcus is all had restraining effect by streptococcus aureus, 1:81 by 1:30.
The root of straight ladybell: taste hardship sweet, micro-, cold nature; Attach to the lung and stomach meridians. Replenishing YIN and removing heat, moistening the lung and resolving the phlegm, reinforcing stomach reg fluid. The main deficiency of Yin is coughed for a long time, and consumptive disease is coughed phlegm blood, and cough caused by dryness phlegm is few, and abnormal heat larynx numbness, Tianjin wound is thirsty.
Spica Prunellae: bitter, pungent, cold in nature; Return liver, gallbladder channel. Clear liver and improve vision, powder detoxifcation. Argument is photophobia also, ophthalmalgia, has a headache dizzy, tinnitus, scrofula, goiter and tumor, acute mastitis, mumps, the swollen poison of carbuncle furuncle, acute and chronic hepatitis, essential hypertension. " medicinal herbs among the people of science " carry: " have diuresis germicidal action. Decoction can wash wound, controls suppurative outer disease. Washing vagina, controls vaginal orifice and uterine mucosa inflammation. " in vitro tests shows, dysentery bacterium, Corynebacterium diphtheriae, vibrio cholerae, intestinal bacteria, Bacillus proteus, staphylococcus and mycobacterium hominis are all had restraining effect in various degree by this product decoction.
Nauclea officinalis: bitter, cold in nature; Return lung, large intestine, courage, bladder warp. Clearing heat and detoxicating, swelling and pain relieving. Main cold, fever, bronchitis, pneumonia, acute tonsillitis, pharyngolaryngitis, mazoitis, enteritis, bacillary dysentery, urinary tract infections, cholecystitis, the ulcer of lower limb, the ringworm of the foot infects, and furuncle swells purulence ulcer, dermatitis and eczema. Modern medicine study confirms,
Being separated the black outer coffin aldehyde alkali obtained from nauclea officinalis stem and show all to have bacteriostatic action with a kind of alkaloid C20H14N2O2 (pomegranate needs to be determined further knot) through bacteriostatic test, wherein golden look staphylococcus, bacillus cereus are had obvious restraining effect at 100 more than �� g/ml by ebony aldehyde alkali. Streptococcus aureus, Bacillus subtilus, intestinal bacteria are all had restraining effect by alkaloid C20H14N2O2.
Tender Catchweed Bedstraw Herb: taste hardship pungent, micro-, cold nature; Return Shao Yin, Taiyin channels. Clearing heat and detoxicating, inducing diuresis for treating stranguria syndrome, swelling and pain relieving. Main swollen ulcer drug, mazoitis, ecphyaditis, oedema, cold, fever, dysentery, urinary tract infections, hematuria, gingival hemorrhage, knife wound is hemorrhage. Jiangxi " herbal medicine handbook " is carried: " clearing heat and detoxicating, swelling and pain relieving. Controlling carbuncle to swell, ecphyaditis, closely for for cancer. " modern medicine study confirmation, streptococcus aureus, intestinal bacteria, shigella etc., by dull and stereotyped paper disk method, are had restraining effect by this product 100% decoction.
Herba Andrographis: bitter, cold in nature; The thoughts of returning home, lung, large intestine, bladder warp. Clearing heat and detoxicating, purging intense heat, eliminating dampness. Main common cold due to wind-heat, warm disease is generated heat, cough due to lung-heat, Whooping cough, lung carbuncle, swelling and pain in the throat, jaundice due to damp-heat, drenches card, erysipelas, and sore carbuncle swells, eczema, venomous snake bite. Guangzhou army " conventional herbal medicine handbook " load: " control acute bacillary dysentery, gastro-enteritis, cat fever, tonsillitis, pneumonia, the swollen poison of sore furuncle, trauma infection contamination, pulmonary tuberculosis, venomous snake bite. " modern medicine study confirmation, Herba Andrographis has eliminating inflammation and expelling toxin effect.
Bracketplant: taste hardship sweet, micro-, cool in nature. Preventing phlegm from forming and stopping coughing, dissipating blood stasis for subsidence of swelling is clearing heat and detoxicating. Main phlegm heat cough, wound, fracture, carbuncle swells, hemorrhoid, burn.
Ligustrum sinense: bitter, cool in nature. Clearing heat and promoting diuresis, detoxify and promote the subsdence of swelling. Main cold, fever, cough due to lung-heat, swelling and pain in the throat, ulceration on the oral mucosa and the tongue, jaundice due to damp-heat, dysentery, carbuncle sore tumefacting virus, eczema, dermatitis, wound, scalds. Bacteriostatic test proves, leaf is to streptococcus aureus, Corynebacterium diphtheriae. Pparatyphoid A bacillus, Pseudomonas aeruginosa, intestinal bacteria, not Shigella flexneri, pneumobacillus have extremely strong anti-microbial effect.
Folium eucalypti: taste is pungent, bitter, cold in nature; Return lung, stomach, spleen, Liver Channel. Expelling wind to resolve the exterior, clearing heat and detoxicating, phlegm-eliminiating and qi-regulating, killing parasites to relieve itching. Main flu, high heat is breathed with cough, Whooping cough, abdominal distention, diarrhoea, dysentery, hook, filaricide, malaria, rheumatalgia, eczema, mange, burn and scald, traumatic hemorrhage. Modern medicine study confirms, isolates a kind of material with column chromatography, gram positive bacterium is had restraining effect, tetanus bacillus and diphtheria corynebacterium toxin is had detoxicating adhesive in vitro from blue gum.
Ternate Grape Fern Herb: taste is sweet, bitter, cool, cold nature; Return lung, Liver Channel. Clearing heat and detoxicating, calming the liver to stop the wind, cough-relieving,
Hemostasis, bright order goes screen. Main infantile hyperpyrexia is frightened jerks, cough due to lung-heat, hemoptysis, and Whooping cough is demented, epilepsy disease, sore swollen toxin, scrofula, venomous snake bite, hot eyes pinkeye, the raw ocular of order. " Bencao Tujing " carries: " treat swollen poison, wind-heat. " modern medicine study confirmation, intestinal bacteria are had restraining effect by the 100% fresh dull and stereotyped paper disk method of grass decoction.
Root of Redcalyx Glorybower: bitter, cold in nature; The thoughts of returning home, lung channel. Heat-clearing, removing toxic substances, cool blood, detumescence. Main cold, fever, cough, pharyngalgia, bleeding from five sense organs or subcutaneous tissue, dysentery characterized by blood in the stool, sore scabies, scrofula, treating swelling and pain by traumatic injury. Guangzhou army " conventional herbal medicine handbook " load: " clearing heat and detoxicating, cough-relieving analgesic therapy. Cure cold fever, pharyngalgia, cough, pulmonary tuberculosis; Stomach trouble, stomachache; Furunculosis furuncle swells. "
Snake contains: bitter, cold in nature; Return liver, lung channel. Heat-clearing is calmed the frightened, and preventing malaria, relieving cough and reducing sputum, removing toxic substances is invigorated blood circulation. Main hyperpyrexia and infantile convulsion, malaria, cough due to lung-heat, hundred crystalline substances are coughed, dysentery, the swollen poison of sore furuncle, swelling and pain in the throat, toothache due to pathogenic wind-fire, zoster, and conjunctival congestion with pain and swelling of the eye, worm snakebite, rheumatism is numb, wound, menoxenia, traumatic hemorrhage. " vegetation is side just " load: " sweating solution flesh. Control wind productive cough to cough, frightened epilepsy, wash eye sterilization. "
Root of Great Burdock: bitter, micro-sweet, cool in nature; Return lung, the heart channel of Hang-Shaoyin. Loose wind-heat, sterilization is swollen. Main common cold due to wind-heat, headache, cough, heat is malicious and swells, swelling and pain in the throat, rheumatic arthralgia, and abdominal mass amasss block, and carbuncle furuncle dislikes sore, hemorrhoid prolapse of the anus. " Tang materia medica " carries: " pin delays weak for main tooth pain, malaria with general debility, wind poison, ulcer, the cough impariment of the lung, and lung is stopped up, hernia lump in the abdomen, long-pending blood. Main all wind, abdominal mass, cold air. "
Gnaphalium affine: taste acid sweet, micro-, property is put down; Return lung channel. Preventing phlegm from forming and stopping coughing, dispels rheumatism, removing toxic substances. Main coughing that to breathe heavily phlegm many, rheumatic arthralgia, has loose bowels, oedema, favism, leucorrhea with red and white discharge, and carbuncle swell furunculosis, and scrotum wets and itches, urticaria, hypertension. " conventional herbal medicine among the people compilation " carries: " disappear larynx fire, antipyretic, removes poison. "
Chinese scholartree branch: bitter, property is put down; The thoughts of returning home, Liver Channel. Loose stasis of blood hemostasis, heat-clearing and damp-drying drug, wind dispelling insecticide. Main uterine bleeding, leucorrhea with red and white discharge, hemorrhoid, scrotum is wet itches, pained, hot eyes, mange. " the southern regions of the Yunnan Province book on Chinese herbal medicine " load: " wash skin scabies leprosy, remove the wind of skin pruritus. "
Radix Cynanchi Paniculati: taste is pungent, warm in nature; Return liver, stomach warp. Wind-expelling pain-stopping, dispellings wind and arresting itching, and removing toxic substances of invigorating blood circulation, unstings antipruritic, detumescence. Main stomach trouble, toothache, rheumatic arthralgia, pain in the back, epigastric pain, toothache, waits for flutter and appoints pain, and urine water conservancy, has loose bowels, dysentery, eczema, urticaria, venomous snake bite. Guangzhou army " conventional herbal medicine handbook " load: " wind-expelling pain-stopping, detoxify and promote the subsdence of swelling, warming channel to dredge meridian. Control venomous snake bite, treating rheumatic ostealgia, heart stomach Qi pain, treating swelling and pain by traumatic injury, zoster, hepatic ascites, menoxenia, dysmenorrhoea. " modern medicine study confirmation, this product
Dull and stereotyped beating hole method and prove, Radix Cynanchi Paniculati is medium sensitivity by streptococcus aureus, and intestinal bacteria, sonne bacillus, Pseudomonas aeruginosa, Corynebacterium diphtheriae are insensitive, and alpha streptococcus is also had restraining effect by Radix Cynanchi Paniculati.
The present invention compared with prior art, has following useful effect:
Selected by medicament of the present invention, medicinal material compatibility is suitable, all medicine phases and there is the advantages such as sterilizing ability is strong, speed of action is fast, stability is strong, cheap, intensive care unit personnel's skin, apparatus and indoor sterilization can be widely used in, human skin is non-stimulated, by rear noresidue, soluble in water, non-corrosiveness, safe without toxic side effect, the working efficiency of medical personnel can be improved, it is a kind of desirable medicament being worthy to be popularized.
Embodiment
Below in conjunction with specific embodiment, the present invention is further elaborated.
Embodiment 1
The raw material of medicament effective constituent of the present invention forms and weight is:
Herbia Wedeliae 88g the root of straight ladybell 86g Spica Prunellae 86g nauclea officinalis 81g
Tender Catchweed Bedstraw Herb 79g Herba Andrographis 79g bracketplant 76g ligustrum sinense 76g
Folium eucalypti 69g Ternate Grape Fern Herb 69g Root of Redcalyx Glorybower 67g snake is containing 63g
Root of Great Burdock 63g Gnaphalium affine 56g Chinese scholartree branch 56g Radix Cynanchi Paniculati 41g.
The preparation method of the present invention, comprises following processing step:
(1) by Herbia Wedeliae, Spica Prunellae, nauclea officinalis, Herba Andrographis, bracketplant, ligustrum sinense, folium eucalypti, Ternate Grape Fern Herb, Root of Redcalyx Glorybower and snake containing putting into container, add 10 times amount distilled water immersions after 4 hours, heated and boiled 4 hours, extract; Second time adds 8 times amount distilled water heated and boiled 3 hours, extracts; Third time adds 6 times amount distilled water heated and boiled 2 hours, extracts; Merge three gained extracting solutions, filter, obtain filtrate for subsequent use;
(2), after the dilution of honey certain amount of boiling water will be refined, add in Root of Upright Ladybell sheet and mix thoroughly, vexed, put in pot, with slow fire heating, fry to yellowish-orange, tack-free is degree, and taking-up cools, and grinds into powder, must be 100 object fine powders, for subsequent use;
(3) Tender Catchweed Bedstraw Herb being smash juice, filter impurity, stays juice, for subsequent use;
(4) after Root of Great Burdock, Gnaphalium affine, Chinese scholartree branch and Radix Cynanchi Paniculati being ground into powder, immersing volumetric concentration is 90% spirituous solution, leaves standstill 4 hours, takes out supernatant liquid, and the medicinal extract precipitated is for subsequent use;
(5) by filtrate made for above-mentioned steps (1)-(4), fine powder, juice and medicinal extract mixing, and add the distilled water of 10-12 times amount, and after stirring and dissolving is even, heated and boiled 1 hour, put less than 60 DEG C, leave standstill 1 hour, filter, obtain filtrate, seal up for safekeeping filling for filtrate, 121 DEG C of pressure sterilizings 30 minutes, packaging, is the preparation method of the described medicament for disinfection of intensive care unit.
Embodiment 2
The raw material of medicament effective constituent of the present invention forms and weight is:
Herbia Wedeliae 81g the root of straight ladybell 79g Spica Prunellae 79g nauclea officinalis 75g
Tender Catchweed Bedstraw Herb 73g Herba Andrographis 73g bracketplant 68g ligustrum sinense 68g
Folium eucalypti 61g Ternate Grape Fern Herb 61g Root of Redcalyx Glorybower 59g snake is containing 56g
Root of Great Burdock 56g Gnaphalium affine 48g Chinese scholartree branch 48g Radix Cynanchi Paniculati 36g.
The present embodiment medicament preparation method's processing step is identical with embodiment 1.
Embodiment 3
The raw material of medicament effective constituent of the present invention forms and weight is:
Herbia Wedeliae 74g the root of straight ladybell 72g Spica Prunellae 72g nauclea officinalis 67g
Tender Catchweed Bedstraw Herb 65g Herba Andrographis 65g bracketplant 62g ligustrum sinense 62g
Folium eucalypti 55g Ternate Grape Fern Herb 55g Root of Redcalyx Glorybower 53g snake is containing 50g
Root of Great Burdock 50g Gnaphalium affine 43g Chinese scholartree branch 43g Radix Cynanchi Paniculati 32g.
The present embodiment medicament preparation method's processing step is identical with embodiment 1.
Embodiment 4
The raw material of medicament effective constituent of the present invention forms and weight is:
Herbia Wedeliae 67g the root of straight ladybell 65g Spica Prunellae 65g nauclea officinalis 61g
Tender Catchweed Bedstraw Herb 59g Herba Andrographis 59g bracketplant 56g ligustrum sinense 56g
Folium eucalypti 49g Ternate Grape Fern Herb 49g Root of Redcalyx Glorybower 47g snake is containing 44g
Root of Great Burdock 44g Gnaphalium affine 38g Chinese scholartree branch 38g Radix Cynanchi Paniculati 27g.
The present embodiment medicament preparation method's processing step is identical with embodiment 1.
Experimental section
The present invention will be selected below to test accordingly, prove effect of the present invention further.
One, soluble test
1, get medicament 10ml of the present invention and put into container, then add distilled water, stir after evenly, filter.
2, after having filtered, filter paper is observed, the obvious residue residual of thing on filter paper.
3, conclusion: medicament of the present invention is extremely soluble in water, it may also be useful to rear noresidue impurity occurs.
Two, toxicity test
1, medicament of the present invention is got
1, acute oral test: the healthy Kunming kind one-level mouse 20 choosing quality 20-22g, male and female half and half, by medicament of the present invention through mouth gastric infusion, administration observes 15 days, records the various performance of mouse and death condition every day, calculates LD. value.
2, cumulative toxicity test: the healthy Kunming kind one-level rat 20 choosing quality 180-200g, male and female half and half, by medicament of the present invention through mouth gastric infusion, administration amount tests the DL. value drawn according to acute oral, the 1-4 days, each administration 0.1LD., within every four hours later, increase progressively 1.5 times of dosage, administration 30 days, and weigh once in every four hours, calculate cumulative coefficient.
3, result: death, does not occur in no abnormality seen reaction after acute oral test mice perfusion, after it is carried out corresponding dissection, tissue or internal organ have no obviously obvious abnormal change.
Cumulative toxicity test administration is after 20 days, and male and female mouse all occurs without death condition, mouse cumulative coefficient 5, the weak cumulative toxicity in position.
Three, skin irritation test
1, healthy white rabbit 4 is selected, physique amount 2.5-3.0kg, the depilation of family's rabbit back backbone both sides is respectively 3.0cm �� 3.0cm, not lesional epidermis, after 24 hours, the stoste of medicament of the present invention for 0.2ml is evenly spread upon the left side of baring skin, right side is not smeared in contrast, after 4 hours, skin wiping is clean, observe skin reaction, carry out stimulating scoring.
2, result: rabbit baring skin reacts there are no erythema and oedema, and skin wound repair integrated value is 0, control sides skin no abnormality seen, by skin wound repair standards of grading, reaction integral mean value is 0, and medicament of the present invention is to no skin irritation.
Four, stability test
Get medicament 50ml of the present invention, after placing 90 days under 37 DEG C of conditions, for streptococcus aureus is carried out sterilization, before its killing effect and storage, compare no significant difference.
Five, bacteriostatic test
Medicament of the present invention is carried out bacteriostatic experiment below:
1, experimental strain:
Select the bacterial classification that laboratory is representative, streptococcus aureus and intestinal bacteria.
2, bacterium culture medium:
Nutrient agar and nutrient broth medium.
3, experiment classification:
Bacteriostatic experiment divides experimental group and control group, and experimental group selects medicament of the present invention, and control group selects iodine to lie prostrate.
4, specific experiment step:
(1) preparation of bacterium liquid: above-mentioned two kinds of bacterial classifications are inoculated on two nutrient agars respectively, cultivate 24 hours at 37 DEG C, choose from above-mentioned nutrient agar respectively and get colonies typical and be inoculated in two fresh nutrient broth mediums respectively, with at temperature, cultivate 20 hours, for subsequent use;
(2) get medicament of the present invention and iodine lies prostrate each 55ml, it is placed in cut-back tank respectively, it is diluted 25 times respectively, above-mentioned two kinds of diluent solution are split in two containers respectively, under being placed in aseptic condition, for subsequent use;
(3) with aseptic nutrient broth, the experimental strain that above-mentioned steps (1) is cultivated 24 hours through nutrient broth being diluted 50 times respectively, often kind of bacterial classification is sub-packed in two culture dish, marks control group and experimental group respectively;
(4) dripping into experimental group culture dish respectively by the diluting soln of two parts of medicaments of the present invention of above-mentioned steps (2) gained, two parts of iodine volt diluting solns drip respectively into control group culture dish.
5, experimental observation is measured:
Above-mentioned experiment is observed, observes two groups of bacterial classifications and measure bacterial classification revolution mark concrete outcome.
6, after observation is measured, leaving standstill two days under above-mentioned culture dish is all placed in aseptic condition, after medicament volatilizees completely, tracing observation finds, control group bacterium colony continues breeding, and experimental group bacterium colony is unchanged.
7, experimental result:
The sterilizing ability of experimental group medicament of the present invention is much larger than the sterilizing ability of control group medicament, and all kinds of bacterial classification can be suppressed to continue breeding.
Claims (5)
1. the medicament for disinfection of intensive care unit and preparation method, it is characterised in that: make described medicament effective constituent raw material composition and weight part number be:
Herbia Wedeliae 60-90 part the root of straight ladybell 58-88 part Spica Prunellae 58-88 part nauclea officinalis 55-83 part
Tender Catchweed Bedstraw Herb 53-81 part Herba Andrographis 53-81 part bracketplant 48-78 part ligustrum sinense 48-78 part
Folium eucalypti 43-71 part Ternate Grape Fern Herb 43-71 part Root of Redcalyx Glorybower 41-69 part snake is containing 38-65 part
Root of Great Burdock 38-65 part Gnaphalium affine 33-58 part Chinese scholartree branch 33-58 part Radix Cynanchi Paniculati 23-43 part.
2. a kind of medicament for disinfection of intensive care unit according to claim 1 and preparation method, it is characterised in that: make described medicament effective constituent raw material composition and weight part number be:
Herbia Wedeliae 81 parts of the root of straight ladybell 79 portions of Spica Prunellaes, 79 portions of nauclea officinalis 75 parts
Tender Catchweed Bedstraw Herb 73 parts of Herba Andrographis 73 portions of bracketplant, 68 parts of ligustrum sinenses 68 parts
Folium eucalypti 61 portions of Ternate Grape Fern Herbs 61 parts of Root of Redcalyx Glorybower, 59 portions of snakes are containing 56 parts
Root of Great Burdock 56 portions of Gnaphalium affines 48 parts of Chinese scholartree branches, 48 parts of Radix Cynanchi Paniculatis 36 parts.
3. a kind of medicament for disinfection of intensive care unit according to claim 1 and preparation method, it is characterised in that: make described medicament effective constituent raw material composition and weight part number be:
Herbia Wedeliae 74 parts of the root of straight ladybell 72 portions of Spica Prunellaes, 72 portions of nauclea officinalis 67 parts
Tender Catchweed Bedstraw Herb 65 parts of Herba Andrographis 65 portions of bracketplant, 62 parts of ligustrum sinenses 62 parts
Folium eucalypti 55 portions of Ternate Grape Fern Herbs 55 parts of Root of Redcalyx Glorybower, 53 portions of snakes are containing 50 parts
Root of Great Burdock 50 portions of Gnaphalium affines 43 parts of Chinese scholartree branches, 43 parts of Radix Cynanchi Paniculatis 32 parts.
4. a kind of medicament for disinfection of intensive care unit according to claim 1 and preparation method, it is characterised in that: make described medicament effective constituent raw material composition and weight part number be:
Herbia Wedeliae 67 parts of the root of straight ladybell 65 portions of Spica Prunellaes, 65 portions of nauclea officinalis 61 parts
Tender Catchweed Bedstraw Herb 59 parts of Herba Andrographis 59 portions of bracketplant, 56 parts of ligustrum sinenses 56 parts
Folium eucalypti 49 portions of Ternate Grape Fern Herbs 49 parts of Root of Redcalyx Glorybower, 47 portions of snakes are containing 44 parts
Root of Great Burdock 44 portions of Gnaphalium affines 38 parts of Chinese scholartree branches, 38 parts of Radix Cynanchi Paniculatis 27 parts.
5. for the preparation method of the medicament of disinfection of intensive care unit as described in an item as arbitrary in claim 1-4, it is characterised in that: comprise following processing step:
(1) by Herbia Wedeliae, Spica Prunellae, nauclea officinalis, Herba Andrographis, bracketplant, ligustrum sinense, folium eucalypti, Ternate Grape Fern Herb, Root of Redcalyx Glorybower and snake containing putting into container, add 10 times amount distilled water immersions after 4 hours, heated and boiled 4 hours, extract; Second time adds 8 times amount distilled water heated and boiled 3 hours, extracts; Third time adds 6 times amount distilled water heated and boiled 2 hours, extracts; Merge three gained extracting solutions, filter, obtain filtrate for subsequent use;
(2), after the dilution of honey certain amount of boiling water will be refined, add in Root of Upright Ladybell sheet and mix thoroughly, vexed, put in pot, with slow fire heating, fry to yellowish-orange, tack-free is degree, and taking-up cools, and grinds into powder, must be 100 object fine powders, for subsequent use;
(3) Tender Catchweed Bedstraw Herb being smash juice, filter impurity, stays juice, for subsequent use;
(4) after Root of Great Burdock, Gnaphalium affine, Chinese scholartree branch and Radix Cynanchi Paniculati being ground into powder, immersing volumetric concentration is 90% spirituous solution, leaves standstill 4 hours, takes out supernatant liquid, and the medicinal extract precipitated is for subsequent use;
(5) by filtrate made for above-mentioned steps (1)-(4), fine powder, juice and medicinal extract mixing, and add the distilled water of 10-12 times amount, and after stirring and dissolving is even, heated and boiled 1 hour, put less than 60 DEG C, leave standstill 1 hour, filter, obtain filtrate, seal up for safekeeping filling for filtrate, 121 DEG C of pressure sterilizings 30 minutes, packaging, is the preparation method of the described medicament for disinfection of intensive care unit.
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Application publication date: 20160601 |