CN111212905A - Rna聚合酶变体 - Google Patents

Rna聚合酶变体 Download PDF

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Publication number
CN111212905A
CN111212905A CN201880066673.3A CN201880066673A CN111212905A CN 111212905 A CN111212905 A CN 111212905A CN 201880066673 A CN201880066673 A CN 201880066673A CN 111212905 A CN111212905 A CN 111212905A
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amino acid
ome
rna
seq
rna polymerase
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Chinese (zh)
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埃米·E·拉比多
阿萨纳西奥斯·杜西斯
干乍那·拉维钱德兰
埃莉萨·霍伯特
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ModernaTx Inc
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ModernaTx Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1247—DNA-directed RNA polymerase (2.7.7.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07006—DNA-directed RNA polymerase (2.7.7.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/31—Chemical structure of the backbone
    • C12N2310/317—Chemical structure of the backbone with an inverted bond, e.g. a cap structure
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
    • C12N2310/352—Nature of the modification linked to the nucleic acid via a carbon atom
    • C12N2310/3521—Methyl
    • C—CHEMISTRY; METALLURGY
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    • C12N2320/00—Applications; Uses
    • C12N2320/50—Methods for regulating/modulating their activity
    • C12N2320/53—Methods for regulating/modulating their activity reducing unwanted side-effects
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/11—Protein-serine/threonine kinases (2.7.11)
    • C12Y207/11023—[RNA-polymerase-subunit] kinase (2.7.11.23)

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  • Engineering & Computer Science (AREA)
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  • Wood Science & Technology (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN201880066673.3A 2017-08-18 2018-08-17 Rna聚合酶变体 Pending CN111212905A (zh)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
US201762547677P 2017-08-18 2017-08-18
US62/547,677 2017-08-18
US201862628484P 2018-02-09 2018-02-09
US62/628,484 2018-02-09
US201862638684P 2018-03-05 2018-03-05
US62/638,684 2018-03-05
US201862677527P 2018-05-29 2018-05-29
US62/677,527 2018-05-29
PCT/US2018/046989 WO2019036682A1 (en) 2017-08-18 2018-08-17 RNA VARIANTS POLYMERASE

Publications (1)

Publication Number Publication Date
CN111212905A true CN111212905A (zh) 2020-05-29

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CN201880066673.3A Pending CN111212905A (zh) 2017-08-18 2018-08-17 Rna聚合酶变体

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US (5) US10526629B2 (https=)
EP (2) EP4397757A3 (https=)
JP (3) JP7408098B2 (https=)
CN (1) CN111212905A (https=)
ES (1) ES2983060T3 (https=)
MA (2) MA49913A (https=)
WO (1) WO2019036682A1 (https=)

Cited By (19)

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CN113795579A (zh) * 2019-02-20 2021-12-14 摩登纳特斯有限公司 用于共转录加帽的rna聚合酶变体
CN114703162A (zh) * 2022-04-24 2022-07-05 江南大学 一种具有高转录活性的t7 rna聚合酶突变体及其应用
CN115975974A (zh) * 2022-07-06 2023-04-18 华中科技大学 适合体外合成rna的t7-rna聚合酶突变体及其应用
CN116179507A (zh) * 2023-03-20 2023-05-30 核芯生物医药科技(杭州)有限公司 一种t7 rna聚合酶突变体及其制备方法和应用
CN116438306A (zh) * 2020-10-20 2023-07-14 St制药株式会社 用于5'-加帽的rna合成的寡核苷酸
CN116926098A (zh) * 2023-07-31 2023-10-24 态创生物科技(广州)有限公司 增强t7rnap对胶原蛋白/弹力蛋白转录活性的定向进化系统和方法
CN117070493A (zh) * 2023-07-05 2023-11-17 上海交通大学 一种具有高热稳定性的t7 rna聚合酶突变体及其应用
CN118755694A (zh) * 2023-07-20 2024-10-11 上海金斯康生物科技有限公司 一种功能改进的t7 rna聚合酶变体
CN118931872A (zh) * 2024-10-14 2024-11-12 南京诺唯赞生物科技股份有限公司 Rna聚合酶变体及其应用
WO2024230665A1 (zh) * 2023-05-09 2024-11-14 南京诺唯赞生物科技股份有限公司 Rna聚合酶变体及其应用
CN119464248A (zh) * 2025-01-14 2025-02-18 昆明医科大学 可用于制备mRNA疫苗的热稳定及转录性能提升的T7 RNA聚合酶突变体及其制备方法
CN119530191A (zh) * 2024-08-21 2025-02-28 湖南大学 一种t7 rna聚合酶突变体及其应用
WO2025113623A1 (en) * 2023-11-29 2025-06-05 Rnasyn Biotech. Co., Ltd. T7 rna polymerase variants and use thereof
CN120349989A (zh) * 2024-01-19 2025-07-22 南京诺唯赞生物科技股份有限公司 T7 rna聚合酶变体及其在rna制备中的应用
WO2025152833A1 (zh) * 2024-01-19 2025-07-24 南京诺唯赞生物科技股份有限公司 Rna聚合酶变体及其应用
WO2025231937A1 (zh) * 2024-05-08 2025-11-13 陈玉松 一种不产生免疫原性副产物、具备高转录活性的t7 rna聚合酶突变体与应用
CN121160664A (zh) * 2024-06-17 2025-12-19 南京诺唯赞生物科技股份有限公司 Rna聚合酶变体
CN121574957A (zh) * 2026-01-27 2026-02-27 南京诺唯赞生物科技股份有限公司 Rna聚合酶变体及其应用
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