CN1066487C - 鉴定植物不育细胞质的基因及用其生产杂交植物的方法 - Google Patents
鉴定植物不育细胞质的基因及用其生产杂交植物的方法 Download PDFInfo
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- CN1066487C CN1066487C CN94190856A CN94190856A CN1066487C CN 1066487 C CN1066487 C CN 1066487C CN 94190856 A CN94190856 A CN 94190856A CN 94190856 A CN94190856 A CN 94190856A CN 1066487 C CN1066487 C CN 1066487C
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8287—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for fertility modification, e.g. apomixis
- C12N15/8289—Male sterility
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/10—Cells modified by introduction of foreign genetic material
- C12N5/12—Fused cells, e.g. hybridomas
- C12N5/14—Plant cells
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S47/00—Plant husbandry
- Y10S47/01—Methods of plant-breeding and including chromosome multiplication
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Abstract
Description
杂交组合 | F1代的可育性 可育个体的基因型 | |
不育1) × 可育2) | 不育: 可育 | 推断 |
KosA2 × Kos1KosA11 × Kos5KosA11 × Kos6KosA8 × Kos7KosA8 × Kos8KosA2 × Xin1KosA1 × Xin2KosA7 × Xin4KosA11 × Xin8KosA2 × Yuan1KosA5 × Yuan6KosA1 × Yuan7KosA12 × Yuan8KosA13 × Yuan9KosA14 × Yuan10KosA15 × Yuan13 | 7 1525 00 226 30 180 250 250 250 250 250 250 250 2511 140 250 25 | RfrfrfrfRfRfRfrfRfRfRfRfRfRfRfRfRfRfRfRfRfRfRfRfRfRfRfrfRfRfRfRf |
杂交组合不育 × 可育 | F1代的可育性分离可育: 不育 | 可育个体的基因型推断 |
cms-KAC × Yuan10cms-KA × Yuan10cms-OGU × Yuan10cms-KAC × Kos6cms-KA × Kos6cms-OGU × Kos6 | 10 05 010 00 825 00 15 | Rf1 Rf2Rf1 Rf2Rf1 Rf2rf1 Rf2rf1 Rf2rf1 Rf2 |
细胞质 | 核的记号 | 核基因组 |
cms-KAC | kos/yuankos | kosena 6 × yuanhong 10kosena 6 |
cms-KA | kos | kosena 5 |
cms-OGU | kos/oguogu | kosena 6 × ogura 3ogura 3 |
cms油菜籽 × 恢复系统 | F1代的可育性可育:不育 |
SW18 × RF138SW18 × RF88 | 84 : 8980 : 71 |
实验次数 | 叶切片周围的不定形芽形成率 | ||
pLAN411 | pKCM125 | pKCMD125 | |
12345 | 0.460.830.331.100.67 | 0.320.13--- | 0.140.020.12-- |
Claims (8)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP269660/1993 | 1993-10-01 | ||
JP26966093 | 1993-10-01 | ||
JP269660/93 | 1993-10-01 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN1115990A CN1115990A (zh) | 1996-01-31 |
CN1066487C true CN1066487C (zh) | 2001-05-30 |
Family
ID=17475445
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN94190856A Expired - Lifetime CN1066487C (zh) | 1993-10-01 | 1994-09-30 | 鉴定植物不育细胞质的基因及用其生产杂交植物的方法 |
Country Status (6)
Country | Link |
---|---|
US (1) | US5866782A (zh) |
EP (1) | EP0675198A4 (zh) |
CN (1) | CN1066487C (zh) |
CA (1) | CA2150667C (zh) |
DE (1) | DE675198T1 (zh) |
WO (1) | WO1995009910A1 (zh) |
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AU5664090A (en) * | 1989-05-05 | 1990-11-29 | Biosource Genetics Corporation | Male sterility in plants |
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- 1994-09-30 WO PCT/JP1994/001625 patent/WO1995009910A1/ja not_active Application Discontinuation
- 1994-09-30 CN CN94190856A patent/CN1066487C/zh not_active Expired - Lifetime
- 1994-09-30 US US08/454,115 patent/US5866782A/en not_active Expired - Lifetime
- 1994-09-30 EP EP94927811A patent/EP0675198A4/en not_active Withdrawn
- 1994-09-30 DE DE0675198T patent/DE675198T1/de active Pending
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WO1992005251A1 (fr) * | 1990-09-21 | 1992-04-02 | Institut National De La Recherche Agronomique | Sequence d'adn conferant une sterilite male cytoplasmique, genome mitochondrial, genome nucleaire, mitochondrie et plante contenant cette sequence, et procede de preparation d'hybrides |
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Also Published As
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CN1115990A (zh) | 1996-01-31 |
EP0675198A1 (en) | 1995-10-04 |
EP0675198A4 (en) | 1996-01-10 |
CA2150667A1 (en) | 1995-04-13 |
US5866782A (en) | 1999-02-02 |
DE675198T1 (de) | 1996-06-27 |
WO1995009910A1 (fr) | 1995-04-13 |
CA2150667C (en) | 2007-01-09 |
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