CN104429781A - Seedling-to-seedling method using tissue culture seedlings of cunninghamia lanceolata - Google Patents
Seedling-to-seedling method using tissue culture seedlings of cunninghamia lanceolata Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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Abstract
The invention discloses a seedling-to-seedling method using tissue culture seedlings of cunninghamia lanceolata. The method has the advantages that a standard cutting orchard is built, two rows of stock plants are arranged symmetrically and are planted and cultivated by enabling trunks to obliquely incline to the center of the ridge surface to form angles of 60-75 degrees with the ground, the stock plants are layered towards the center of the ridge surface by hooking portions, 30-50cm in height, of the stock plants by steel hooks after 7-8 months of stable growth of the stock plants, and angles of 33-50 degrees are formed between the trunks of the stock plants and the ground, so that damage to the stock plants is avoided, the stock plants do not affect one another, illumination is reasonable, and management is facilitated; hormones are sprayed to the roots of the stock plants to promote germination, so that substantial high-quality coppice shoots uniform in specification can be obtained to serve as cutting slips, orderly cutting nursery stocks can be cultivated, and propagation coefficients of the nursery stocks are increased effectively; special seedling cultivation equipment is not needed, simplicity and easiness in operation are achieved, technical requirements are low, and seedling cultivation cost and time are greatly saved; seedling cultivation is performed by vegetative propagation of cuttings, so that excellent inheritable characters of excellent varieties of the cunninghamia lanceolata are kept well, and high economic, social and ecological benefits are achieved.
Description
technical field:
The invention belongs to technical field of plant asexual propagation, relate to a kind of China fir plantlet in vitro that adopts with the method for Miao Fanmiao.
background technology:
China fir (
cunninghamia lanceolata) be under the jurisdiction of Taxodiaceae Cunninghamia, being one of distinctive evergreen needle commerical tree species of China, is also one of the Major Tree Species Planted in south China area.China fir has the cultivation history of more than 1,000 year in China, plays an important role in forest development, can be rated as southern china " king in wood ".Growth of Chinese Fir is fast, output is high, distribution is wide, and its wood quality gently tough, even structure, texture leads to directly, is easy to processing, is not only good furniture woods, construction timber, is also excellent paper pulp material, enjoys liking of broad masses.China fir is as the Major Tree Species Planted of south China, and area under cultivation constantly increases, and supply falls short of demand for breeding nursery stock, along with the propelling of Elite Variety Cultivation of Chinese Fir process, high-quality breeding strong sprout particularly choiceness nursery stock demand by increasing.Therefore, greatly develop Growing Seedling of China Fir technology, the market demand meeting high quality seedling is one of major issue faced at present.
Recent years, China Fir researcher does a lot of work in the technical research of China fir asexual grow seedling, as CN l03875426 A, and-kind of the method that promotion China fir foundation is sprouted, select the positive north of China fir foundation or Due South to, apart from foundation horizontal range 20-25cm place hard straight cutting one supporting lever; China fir trunk is slowly bent to supporting lever direction, until China fir trunk and ground form 20-30
°angle; By the infall rope tighten of China fir and supporting lever; China fir trunk portion below infall cloth is wound around l-2 circle; The thick l-2 cm of Nutrition Soil is covered at China fir foundation place; The coppice shoot at clip China fir foundation place is used for vegetative propagation; The method can obtain the China fir explant (fringe bar) of high yield, high-quality, but maternal plant vertically to plant its press strip process required time longer, simultaneously larger with soil space needed for same direction plantation maternal plant and bad management and adopt fringe.The tissue culture technique of current China fir have also been obtained and develops on a large scale very much, has started China fir group training industrialization nursery in indivedual province.Although the training of China fir group has formed industrialization nursery, but its cost is high, technical requirement is complicated, and up to the present tissue culture technology popularity is still not high, a lot of province still adopts the most traditional type of seeding nursery, and high-quality breeding nursery stock output still can not be met the need of market.
Plantlet in vitro is adopted to be the another kind asexual grow seedling method fast and efficiently except group training asexual grow seedling with seedling seedling-breeding method, adopt the method not only can obtain higher reproduction coefficient, and greatly shorten seedling raise period and save seedling cost, and effectively can maintain superior families or clonal excellent genetic character, be one of effective way solving current China fir seedling-wood breeding problem.The current not Patents of this technical scheme and the report of document.
summary of the invention:
The object of the invention is to for current China fir breeding nursery stock markets that supply falls short of demand, the actual conditions that particularly breeding clone nursery stock is extremely in short supply, provide a kind of reproduction coefficient is high, seedling raise period is short, seedling cost is cheap, merit is stablized and be easy to promote the use of employing China fir plantlet in vitro with the method for Miao Fanmiao.
Technical scheme of the present invention is:
A kind of China fir plantlet in vitro that adopts is with the method for Miao Fanmiao, comprise maternal plant selection, cutting orchard foundation, cutting orchard manager pay, nursery lot select with wholely, adopt fringe and the operation such as cuttage, cuttage seeding management, it is characterized in that: with China fir plantlet in vitro for mother plant for cutting material, set up China fir cutting orchard, carry on the work to cutting orchard and manage, promote that maternal plant base portion budling sprouts, cuttage and seedling culture is carried out in the rudiment of clip high-quality, obtain a large amount of high-quality cuttage nursery stock, concrete operation step is as follows:
(1) maternal plant is selected: select 1-2 raw, high-quality, without damage by disease and insect China fir plantlet in vitro as mother plant for cutting;
(2) cutting orchard set up: select sunny, not ponding but near have the plot at water source as cutting orchard garden ground, carry out planting with maternal plant wholely;
(3) cutting orchard operation and management: after mother plant for cutting is planted in step (2), optionally carry out according to a conventional method watering and weeding, regularly carry out spray medicine, fertilising, pruning, press strip, bud picking and foundation and urge to sprout;
(4) nursery lot is selected and whole: select sunny, not ponding but near have the plot at water source as cuttage nursery lot, whole and routine disinfection process is carried out to nursery lot;
(5) fringe and cuttage is adopted: in 1-5 month and 10-12 month clip high-quality coppice shoot as cuttings, cuttings, after the process of root-growing agent conventional method, is 8-10 cm with seeding row spacing and carries out cuttage;
(6) cuttage seeding management: after tloig-cutlage, carry out daily management according to cuttage seeding management method.
Above step (2) cutting orchard set up described in comprise weeding wholely, dig drain ditch and build Kui face, drain ditch is around cutting orchard surrounding, and Kui face width 1.0-1.2 m, high 5-10 cm, Kui and Kui spacing are 40-60 cm.
It is two row maternal plants of planting on each Kui face that maternal plant described in the foundation of above step (2) cutting orchard is planted, maternal plant line space is 60-80 cm, strain spacing is 30-40 cm, maternal plant is formed into 60-75 degree angle with the oblique Kui face central authorities of the symmetrical trunk of two row with ground and plants, and before planting, every strain maternal plant uses 40-50 g composite fertilizer as base manure.
Spray medicine described in above step (3) cutting orchard operation and management sprays conventional bactericide and insecticide, bactericide spraying method sprayed once every 20-30 days, various sterilization agent takes turns to use, and spraying concentration is determined according to the operation instruction of different bactericide, optionally sprays insecticide.
Fertilising described in above step (3) cutting orchard operation and management is applied fertilizer with dried human and animal excreta and liquid manure two kinds of modes, monthly respectively use composite fertilizer once, wherein: before rainy day or rainy day arrive, select dried human and animal excreta to spread fertilizer over the fields, evenly composite fertilizer is spread fertilizer over the fields around maternal plant base portion, in the Season select dried human and animal excreta ditch spread that rainfall is less, groove is excavated outside every row maternal plant, fertilizer is evenly sprinkling upon in groove, then return soil to cover, select to use liquid manure at arid season, drench around maternal plant base portion and execute composite fertilizer's liquid manure that concentration is 3-5 %, the consumption of composite fertilizer is 30-50 kg/acre, irrigate in right amount after fertilising.
Pruning described in above step (3) cutting orchard operation and management be by liftoff for maternal plant mobile jib 20 cm within branch wipe out; Described bud picking is erased at the rudiment of liftoff for maternal plant mobile jib 10 more than cm; Described press strip be maternal plant plant growth in 7-8 month stable after, in maternal plant 30-50 cm eminence, with cleek, the maternal plant of two row is carried out press strip to Kui face centre, makes maternal plant trunk and ground form 33-50 degree angle, can not maternal plant be damaged.
Foundation described in above step (3) cutting orchard operation and management is short to be sprouted is adopt concentration to be that 0.2-0.5 mg/L 6-benzyl aminoadenine (6-BA) solution sprays mobile jib within liftoff 10 cm of maternal plant and root position, promotes that budling sprouts.
Above step (4) nursery lot is selected and comprising weeding wholely, dig drain ditch and build Kui face described in whole ground, drain ditch around garden ground surrounding, Kui face width 1.0-1.2 m, high 15-25 cm, Kui face covers one deck yellow soil, and the spacing of Kui Yukui is 40-60 cm.
It is select length to be 5-10 cm that above step (5) adopts the clip high-quality coppice shoot described in fringe and cuttage, sturdy and without the coppice shoot of damage by disease and insect, coppice shoot is cut as cuttings in most base position, length is greater than the coppice shoot of 10 cm, coppice shoot is cut off part stem section again after the most base portion of maternal plant is cut, and making length is the long coppice shoot of 5-10 cm.
To be that cuttage is complete water and spray bactericide cuttage seeding management method described in the management of above step (6) cuttage seeding immediately, the shade net that obscurity is 80 % is added a cover after cuttage, water every day more than 2 times, sprayed bactericide once every 7-10 days, various sterilization agent takes turns to use, after seedling root growth in cuttage 40-45 days is stable, liquid manure is executed once every 10-15 days, grow vigorous season nitrogenous fertilizer and composite fertilizer alternately use, optionally weeding, raised shade net after October.
Advantage of the present invention and good effect as follows:
1. this employing China fir plantlet in vitro is with the method for Miao Fanmiao, by setting up the cutting orchard of specification, maternal plant trunk and ground are formed plants with two row symmetrical inclineds carry out coppice shoot cultivation to Kui face central authorities 60-75 degree angle, until maternal plant plant growth in 7-8 month stable after, in maternal plant 30-50 cm eminence, with cleek, the maternal plant of two row is carried out press strip to Kui face centre, maternal plant trunk and ground is made to form 33-50 degree angle, maternal plant can not be damaged, maternal plant is independent of each other, illumination is reasonable, convenient management, a large amount of high-quality can be obtained and the consistent coppice shoot of specification as cuttings, cultivate neat cuttage nursery stock, effectively improve seedling propagation coefficient simultaneously.
2. this employing China fir plantlet in vitro is with the method for Miao Fanmiao, do not need special seedling equipment, its culture facility requires very simple, operating procedure is simple simultaneously, technical requirement is low, not only greatly save seedling cost and time, and easily accepted by broad masses, be conducive to extensively promoting the use of.
3. this employing China fir plantlet in vitro is with the method for Miao Fanmiao, carries out cultivating seedlings by cuttage vegetative manner, maintains the excellent genetic character of excellent China fir kind preferably, has good economic benefit, social benefit and ecological benefits.
Accompanying drawing explanation
Fig. 1: cutting orchard maternal plant is planted view.
Identify in figure: 1-garden Kui, 2-maternal plant, 3-cleek.
Embodiment
Below in conjunction with accompanying drawing specific embodiment, the invention will be further described.
embodiment 1:
Select sunny, not ponding, near have the plot at water source as cutting orchard ground, garden ground surrounding dig drain ditch, build the Kui face of wide 1.0 m, high 5-10 cm, the spacing of Kui Yukui is 40 cm, China fir high-quality plantlet in vitro is selected to be mother plant for cutting February, maternal plant trunk and ground are formed 65-70 degree angle oblique Kui face central authorities to plant, before planting, every strain uses 40-50 g composite fertilizer as base manure, maternal plant line space is 60 cm, strain spacing is 40 cm, maternal plant sprayed bactericide once every 20-30 days after planting, and was taken turns to use by the bactericide such as carbendazim, thiophanate methyl, fenaminosulf, mancozeb, tpn, monthly adopt composite fertilizer to apply fertilizer to maternal plant, rainy day or rainy day arrive before carry out dried human and animal excreta and spread fertilizer over the fields, rainfall carries out dried human and animal excreta ditch spread at less season, and arid season uses liquid manure, and fertilising consumption is 30-50 kg/acre, monthly carry out a bud picking process, in pruning and press strip process to maternal plant October then, press strip method is in maternal plant 40-45 cm eminence, with cleek, the maternal plant of two row is carried out press strip to Kui face centre, make maternal plant trunk and ground form 40-45 degree angle, November, January next year, 0.2 mg/L, 0.4mg/L 6-benzyl aminoadenine solution is sprayed respectively to maternal plant base portion simultaneously, the long fringe bar of 5-10 cm is gathered March next year, fringe bar carries out cuttage after rooting treatment, cuttage seeding row spacing is 8-10 cm, cuttage nursery lot surrounding digs drain ditch, cuttage Kui face width 1.0 cm, high 15 cm, Kui face covers yellow soil, before cuttage, routine disinfection is carried out to Kui face, distance between Kui Yukui is 40 cm, water immediately after cuttage and spray bactericide and add a cover the shade net that obscurity is 80 %, every day optionally waters 2-3 time, bactericide is sprayed once every 7-10 days, by carbendazim, thiophanate methyl, fenaminosulf, mancozeb, the bactericide such as tpn take turns to use, cuttage starts fertilising after 40 days, every 10-15 days sealing fertilizer liquid manure once, optionally weeding, shade net is opened after Cold Dew, cuttage survival rate 86.5 %.
embodiment 2:
Select sunny, not ponding, near have the plot at water source as cutting orchard ground, garden ground surrounding dig drain ditch, build the Kui face of wide 1.2 m, high 5-10 cm, the spacing of Kui Yukui is 60 cm, China fir high-quality plantlet in vitro is selected to be mother plant for cutting February, maternal plant trunk and ground are formed 60-65 degree angle oblique Kui face central authorities to plant, before planting, every strain uses 40-50 g composite fertilizer as base manure, maternal plant line space is 60 cm, strain spacing is 40 cm, maternal plant sprayed bactericide once every 20-30 days after planting, and was taken turns to use by the bactericide such as carbendazim, thiophanate methyl, fenaminosulf, mancozeb, tpn, monthly adopt composite fertilizer to apply fertilizer to maternal plant, rainy day or rainy day arrive before carry out dried human and animal excreta and spread fertilizer over the fields, rainfall carries out dried human and animal excreta ditch spread at less season, and arid season uses liquid manure, and fertilising consumption is 30-50 kg/acre, monthly carry out a bud picking process, in pruning and press strip process to maternal plant October then, press strip method is in maternal plant 45-50 cm eminence, with cleek, the maternal plant of two row is carried out press strip to Kui face centre, make maternal plant trunk and ground form 33-38 degree angle, November, January next year, 0.3 mg/L, 0.5 mg/L 6-benzyl aminoadenine solution are sprayed respectively to maternal plant base portion simultaneously, the long fringe bar of 5-10 cm is gathered March next year, fringe bar carries out cuttage after rooting treatment, cuttage seeding row spacing is 8-10 cm, cuttage nursery lot surrounding digs drain ditch, cuttage Kui face width 1.2 cm, high 20 cm, Kui face covers yellow soil, before cuttage, routine disinfection is carried out to Kui face, distance between Kui Yukui is 60 cm, water immediately after cuttage and spray bactericide and add a cover the shade net that obscurity is 80 %, every day optionally waters 2-3 time, bactericide is sprayed once every 7-10 days, by carbendazim, thiophanate methyl, fenaminosulf, mancozeb, the bactericide such as tpn take turns to use, cuttage starts fertilising after 45 days, every 10-15 days sealing fertilizer liquid manure once, optionally weeding, shade net is opened after October, cuttage survival rate 87.7 %.
embodiment 3:
Select sunny, not ponding, near have the plot at water source as cutting orchard ground, garden ground surrounding dig drain ditch, build the Kui face of wide 1.2 m, high 5-10 cm, the spacing of Kui Yukui is 60 cm, China fir high-quality plantlet in vitro is selected to be mother plant for cutting February, maternal plant trunk and ground are formed 65-70 degree angle oblique Kui face central authorities to plant, before planting, every strain uses 40-50 g composite fertilizer as base manure, maternal plant line space is 80 cm, strain spacing is 30 cm, maternal plant sprayed bactericide once every 20-30 days after planting, and was taken turns to use by the bactericide such as carbendazim, thiophanate methyl, fenaminosulf, mancozeb, tpn, monthly adopt composite fertilizer to apply fertilizer to maternal plant, rainy day or rainy day arrive before carry out dried human and animal excreta and spread fertilizer over the fields, rainfall carries out dried human and animal excreta ditch spread at less season, and arid season uses liquid manure, and fertilising consumption is 30-50 kg/acre, monthly carry out a bud picking process, in pruning and press strip process to maternal plant October then, press strip method is in maternal plant 30-35 cm eminence, with cleek, the maternal plant of two row is carried out press strip to Kui face centre, making maternal plant trunk and ground form 40-45 degree angle, is simultaneously 0.3 mg/L 6-benzyl aminoadenine solution November, January next year to maternal plant base portion spraying concentration, the long fringe bar of 5-10 cm is gathered April next year, fringe bar carries out cuttage after rooting treatment, cuttage seeding row spacing is 8-10 cm, cuttage nursery lot surrounding digs drain ditch, cuttage Kui face width 1.2 cm, high 25 cm, Kui face covers yellow soil, before cuttage, routine disinfection is carried out to Kui face, distance between Kui Yukui is 60 cm, water immediately after cuttage and spray bactericide and add a cover the shade net that obscurity is 80 %, every day optionally waters 2-3 time, bactericide is sprayed once every 7-10 days, by carbendazim, thiophanate methyl, fenaminosulf, mancozeb, the bactericide such as tpn take turns to use, cuttage starts fertilising after 45 days, every 10-15 days sealing fertilizer liquid manure once, optionally weeding, shade net is opened after October, cuttage survival rate 90.8 %.
embodiment 4:
Select sunny, not ponding, near have the plot at water source as cutting orchard ground, garden ground surrounding dig drain ditch, build the Kui face of wide 1.2 m, high 5-10 cm, the spacing of Kui Yukui is 60 cm, China fir high-quality plantlet in vitro is selected to be mother plant for cutting February, maternal plant trunk and ground are formed 60-65 degree angle oblique Kui face central authorities to plant, before planting, every strain uses 40-50 g composite fertilizer as base manure, maternal plant line space is 80 cm, strain spacing is 30 cm, maternal plant sprayed bactericide once every 20-30 days after planting, and was taken turns to use by the bactericide such as carbendazim, thiophanate methyl, fenaminosulf, mancozeb, tpn, monthly adopt composite fertilizer to apply fertilizer to maternal plant, rainy day or rainy day arrive before carry out dried human and animal excreta and spread fertilizer over the fields, rainfall carries out dried human and animal excreta ditch spread at less season, and arid season uses liquid manure, and fertilising consumption is 30-50 kg/acre, monthly carry out a bud picking process, in pruning and press strip process to maternal plant October then, press strip method is in maternal plant 30-40 cm eminence, with cleek, the maternal plant of two row is carried out press strip to Kui face centre, maternal plant trunk and ground is made to form 45-50 degree angle, simultaneously November then, January next year, March, October and in January, the 3rd, March, 6 6-benzyl aminoadenine solution cutting handle are carried out to maternal plant base portion, front twice spraying concentration is 0.3 mg/L, after 4 spraying concentrations be 0.4 mg/L, the long fringe article of 5-10 cm is gathered in April, the 3rd, fringe bar carries out cuttage after rooting treatment, cuttage seeding row spacing is 8-10 cm, cuttage nursery lot surrounding digs drain ditch, cuttage Kui face width 1.2 cm, high 25 cm, Kui face covers yellow soil, before cuttage, routine disinfection is carried out to Kui face, distance between Kui Yukui is 60 cm, water immediately after cuttage and spray bactericide and add a cover the shade net that obscurity is 80 %, every day optionally waters 2-3 time, bactericide is sprayed once every 7-10 days, by carbendazim, thiophanate methyl, fenaminosulf, mancozeb, the bactericide such as tpn take turns to use, cuttage starts fertilising after 45 days, every 10-15 days sealing fertilizer liquid manure once, optionally weeding, shade net is opened after Cold Dew, cuttage survival rate 91.9 %.
Claims (10)
1. one kind adopts China fir plantlet in vitro with the method for Miao Fanmiao, comprise maternal plant selection, cutting orchard foundation, cutting orchard manager pay, nursery lot select with wholely, adopt fringe and the operation such as cuttage, cuttage seeding management, it is characterized in that: with China fir plantlet in vitro for mother plant for cutting material, set up China fir cutting orchard, carry on the work to cutting orchard and manage, promote that maternal plant base portion budling sprouts, cuttage and seedling culture is carried out in the rudiment of clip high-quality, obtain a large amount of high-quality cuttage nursery stock, concrete operation step is as follows:
(1) maternal plant is selected: select 1-2 raw, high-quality, without damage by disease and insect China fir plantlet in vitro as mother plant for cutting;
(2) cutting orchard set up: select sunny, not ponding but near have the plot at water source as cutting orchard garden ground, carry out planting with maternal plant wholely;
(3) cutting orchard operation and management: after mother plant for cutting is planted in step (2), optionally carry out according to a conventional method watering and weeding, regularly carry out spray medicine, fertilising, pruning, press strip, bud picking and foundation and urge to sprout;
(4) nursery lot is selected and whole: select sunny, not ponding but near have the plot at water source as cuttage nursery lot, whole and routine disinfection process is carried out to nursery lot;
(5) fringe and cuttage is adopted: in 1-5 month and 10-12 month clip high-quality coppice shoot as cuttings, cuttings, after the process of root-growing agent conventional method, is 8-10 cm with seeding row spacing and carries out cuttage;
(6) cuttage seeding management: after tloig-cutlage, carry out daily management according to cuttage seeding management method.
2. a kind of China fir plantlet in vitro that adopts according to claim 1 is with the method for Miao Fanmiao, it is characterized in that: step (2) cutting orchard set up described in comprise weeding wholely, dig drain ditch and build Kui face, drain ditch is around cutting orchard surrounding, Kui face width 1.0-1.2 m, high 5-10 cm, Kui and Kui spacing are 40-60 cm.
3. a kind of China fir plantlet in vitro that adopts according to claim 1 is with the method for Miao Fanmiao, it is characterized in that: it is two row maternal plants of planting on each Kui face that the maternal plant described in the foundation of step (2) cutting orchard is planted, maternal plant line space is 60-80 cm, strain spacing is 30-40 cm, maternal plant forms 60-75 degree angle with the oblique Kui face central authorities of the symmetrical trunk of two row with ground and plants, and before planting, every strain maternal plant uses 40-50 g composite fertilizer as base manure.
4. a kind of China fir plantlet in vitro that adopts according to claim 1 is with the method for Miao Fanmiao, it is characterized in that: the spray medicine described in step (3) cutting orchard operation and management sprays conventional bactericide and insecticide, bactericide spraying method sprayed once every 20-30 days, various sterilization agent takes turns to use, spraying concentration is determined according to the operation instruction of different bactericide, optionally sprays insecticide.
5. a kind of China fir plantlet in vitro that adopts according to claim 1 is with the method for Miao Fanmiao, it is characterized in that: the fertilising described in step (3) cutting orchard operation and management is applied fertilizer with dried human and animal excreta and liquid manure two kinds of modes, monthly respectively use composite fertilizer once, wherein: before rainy day or rainy day arrive, select dried human and animal excreta to spread fertilizer over the fields, evenly composite fertilizer is spread fertilizer over the fields around maternal plant base portion, in the Season select dried human and animal excreta ditch spread that rainfall is less, groove is excavated outside every row maternal plant, fertilizer is evenly sprinkling upon in groove, then return soil to cover, select to use liquid manure at arid season, drench around maternal plant base portion and execute composite fertilizer's liquid manure that concentration is 3-5 %, the consumption of composite fertilizer is 30-50 kg/acre, irrigate in right amount after fertilising.
6. a kind of China fir plantlet in vitro that adopts according to claim 1 is with the method for Miao Fanmiao, it is characterized in that: the pruning described in step (3) cutting orchard operation and management be by liftoff for maternal plant mobile jib 20 cm within branch wipe out; Described bud picking is erased at the rudiment of liftoff for maternal plant mobile jib 10 more than cm; Described press strip be maternal plant plant growth in 7-8 month stable after, in maternal plant 30-50 cm eminence, with cleek, the maternal plant of two row is carried out press strip to Kui face centre, makes maternal plant trunk and ground form 33-50 degree angle, can not maternal plant be damaged.
7. a kind of China fir plantlet in vitro that adopts according to claim 1 is with the method for Miao Fanmiao, it is characterized in that: the foundation described in step (3) cutting orchard operation and management is short to be sprouted is adopt concentration to be that 0.2-0.5 mg/L 6-benzyl aminoadenine (6-BA) solution sprays mobile jib within liftoff 10 cm of maternal plant and root position, promotes that budling sprouts.
8. a kind of China fir plantlet in vitro that adopts according to claim 1 is with the method for Miao Fanmiao, it is characterized in that: step (4) nursery lot is selected and comprising weeding wholely, dig drain ditch and build Kui face described in whole ground, drain ditch is around ground, garden surrounding, Kui face width 1.0-1.2 m, high 15-25 cm, Kui face covers one deck yellow soil, and the spacing of Kui Yukui is 40-60 cm.
9. a kind of China fir plantlet in vitro that adopts according to claim 1 is with the method for Miao Fanmiao, it is characterized in that: it is select length to be 5-10 cm that step (5) adopts the clip high-quality coppice shoot described in fringe and cuttage, sturdy and without the coppice shoot of damage by disease and insect, coppice shoot is cut as cuttings in most base position, length is greater than the coppice shoot of 10 cm, coppice shoot is cut off part stem section again after the most base portion of maternal plant is cut, and making length is the long coppice shoot of 5-10 cm.
10. a kind of China fir plantlet in vitro that adopts according to claim 1 is with the method for Miao Fanmiao, it is characterized in that: to be that cuttage is complete water and spray bactericide the cuttage seeding management method described in the management of step (6) cuttage seeding immediately, the shade net that obscurity is 80 % is added a cover after cuttage, water every day more than 2 times, bactericide is sprayed once every 7-10 days, various sterilization agent takes turns to use, after seedling root growth in cuttage 40-45 days is stable, liquid manure is executed once every 10-15 days, grow vigorous season nitrogenous fertilizer and composite fertilizer alternately use, optionally weeding, after October, shade net is raised.
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| CN104920146A (en) * | 2015-06-15 | 2015-09-23 | 广西壮族自治区林业科学研究院 | Propagation method for rapid spikelet breeding of ithocarpus polysachyus rehd |
| CN108076951A (en) * | 2018-01-23 | 2018-05-29 | 福建农林大学 | A kind of China fir mother plant for cutting yield regulates and controls method |
| CN108496682A (en) * | 2018-03-30 | 2018-09-07 | 赣州市森源科技种苗场 | A kind of long-lived Clones of Cunninghamia Lanceolata cutting orchard construction method of high yield |
| CN108617385A (en) * | 2018-05-09 | 2018-10-09 | 福建林业职业技术学院 | A kind of China fir band hat buries the construction method of dry method cutting orchard |
| CN110463501A (en) * | 2019-09-19 | 2019-11-19 | 云南省林业科学院 | A kind of above Simao pine cuttage fringe bar rush of triennial sprouts method |
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| CN114902883A (en) * | 2022-05-16 | 2022-08-16 | 南京林业大学 | Method for intensively producing cutting shoots and twigs of catalpa bungei |
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| CN104904542A (en) * | 2015-06-01 | 2015-09-16 | 广西壮族自治区林业科学研究院 | Method for building cutting orchard with Pinus massoniana Lamb. tissue culture seedlings |
| CN104904542B (en) * | 2015-06-01 | 2017-06-06 | 广西壮族自治区林业科学研究院 | A kind of method that utilization masson pine tissue-cultured seedling builds cutting orchard |
| CN104920146A (en) * | 2015-06-15 | 2015-09-23 | 广西壮族自治区林业科学研究院 | Propagation method for rapid spikelet breeding of ithocarpus polysachyus rehd |
| CN104920146B (en) * | 2015-06-15 | 2017-08-11 | 广西壮族自治区林业科学研究院 | A kind of Pasania cuspidata quickly educates the propagation method of fringe |
| CN108076951A (en) * | 2018-01-23 | 2018-05-29 | 福建农林大学 | A kind of China fir mother plant for cutting yield regulates and controls method |
| CN108496682A (en) * | 2018-03-30 | 2018-09-07 | 赣州市森源科技种苗场 | A kind of long-lived Clones of Cunninghamia Lanceolata cutting orchard construction method of high yield |
| CN108617385A (en) * | 2018-05-09 | 2018-10-09 | 福建林业职业技术学院 | A kind of China fir band hat buries the construction method of dry method cutting orchard |
| CN110463501A (en) * | 2019-09-19 | 2019-11-19 | 云南省林业科学院 | A kind of above Simao pine cuttage fringe bar rush of triennial sprouts method |
| CN110463501B (en) * | 2019-09-19 | 2022-03-29 | 云南省林业科学院 | Scion germination promoting method for cutting pinus khasys of 3 years old or older |
| CN113854027A (en) * | 2021-10-09 | 2021-12-31 | 延边朝鲜族自治州农业科学院（延边特产研究所） | Asexual propagation method of wild hazel and hazel fruit trees |
| CN113875475A (en) * | 2021-10-09 | 2022-01-04 | 延边朝鲜族自治州农业科学院（延边特产研究所） | Asexual propagation method of wild corylus heterophylla bunge fruit tree |
| CN114902883A (en) * | 2022-05-16 | 2022-08-16 | 南京林业大学 | Method for intensively producing cutting shoots and twigs of catalpa bungei |
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