CN103080308B - 底物特异性得到改变的阿马多里酶 - Google Patents

底物特异性得到改变的阿马多里酶 Download PDF

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CN103080308B
CN103080308B CN201180038922.6A CN201180038922A CN103080308B CN 103080308 B CN103080308 B CN 103080308B CN 201180038922 A CN201180038922 A CN 201180038922A CN 103080308 B CN103080308 B CN 103080308B
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amino acid
amadoriase
seq
replaced
εfk
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CN103080308A (zh
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一柳敦
广川浩三
田锅康子
钺阳介
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Kitsu Kou Man K K
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0026—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5)
    • C12N9/0032—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5) with oxygen as acceptor (1.5.3)
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
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    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/72—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood pigments, e.g. haemoglobin, bilirubin or other porphyrins; involving occult blood
    • G01N33/721—Haemoglobin
    • G01N33/723—Glycosylated haemoglobin
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/902—Oxidoreductases (1.)

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CN201180038922.6A 2010-08-06 2011-08-04 底物特异性得到改变的阿马多里酶 Active CN103080308B (zh)

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JP2010176967 2010-08-06
JP2010-176967 2010-08-06
JP2010-213070 2010-09-24
JP2010213070 2010-09-24
PCT/JP2011/067898 WO2012018094A1 (ja) 2010-08-06 2011-08-04 基質特異性が改変されたアマドリアーゼ

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CN103080308A CN103080308A (zh) 2013-05-01
CN103080308B true CN103080308B (zh) 2015-08-05

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US (1) US9062286B2 (https=)
EP (1) EP2602318B1 (https=)
JP (2) JP6176922B2 (https=)
KR (1) KR102070990B1 (https=)
CN (1) CN103080308B (https=)
WO (1) WO2012018094A1 (https=)

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JP6282115B2 (ja) 2011-12-28 2018-02-21 キッコーマン株式会社 熱安定性が向上したアマドリアーゼ、その遺伝子および組換えｄｎａならびに熱安定性が向上したアマドリアーゼの製造法
JP6068916B2 (ja) * 2012-02-02 2017-01-25 キッコーマン株式会社 糖化ヘモグロビン測定用試薬組成物および糖化ヘモグロビン測定方法
WO2013162035A1 (ja) 2012-04-27 2013-10-31 キッコーマン株式会社 フルクトシルヘキサペプチドに作用する改変型アマドリアーゼ
CN103937763B (zh) * 2013-01-22 2016-01-06 清华大学 R112w型酮胺氧化酶及其编码基因和应用
JP6363327B2 (ja) * 2013-03-25 2018-07-25 キッコーマン株式会社 改変アマドリアーゼ
ES2848705T3 (es) 2013-08-09 2021-08-11 Kikkoman Corp Amadoriasa modificada y método para producirla, agente para mejorar la resistencia a tensioactivos de la amadoriasa y composición para medir la HbA1c usando la misma
JP6542671B2 (ja) * 2013-10-25 2019-07-10 キッコーマン株式会社 ヘモグロビンＡ１ｃの測定方法および測定キット
CN105683390B (zh) * 2013-10-25 2021-02-26 龟甲万株式会社 使用作用于糖基化肽的阿马多里酶的HbA1c测定方法
JP6749745B2 (ja) * 2014-02-27 2020-09-02 キッコーマン株式会社 アマドリアーゼ含有組成物の安定化方法、熱安定性が向上したアマドリアーゼ含有組成物及びこれを用いた糖化ヘモグロビン測定用組成物
EP3786291B1 (en) 2014-10-24 2023-10-18 Kikkoman Corporation Amadoriase having enhanced dehydrogenase activity
WO2016072520A1 (ja) * 2014-11-07 2016-05-12 キッコーマン株式会社 アニオン性界面活性剤耐性が向上したアマドリアーゼ
CN107002074B (zh) * 2015-06-10 2021-08-10 公立大学法人富山县立大学 活性型突变酶的制造方法及活性型突变酶、以及可溶性化突变蛋白的制造方法
KR102596402B1 (ko) * 2016-04-22 2023-10-31 기꼬만 가부시키가이샤 HbA1c 디히드로게나아제
TWI668229B (zh) 2016-12-13 2019-08-11 財團法人工業技術研究院 重組蛋白質及其製造方法與應用
EP3660156A4 (en) * 2017-07-24 2021-03-31 Riken DECARBOXYLASE AND METHOD FOR PRODUCING UNSATURATED HYDROCARBONS USING THE SAME
JP7157063B2 (ja) 2017-08-31 2022-10-19 キッコーマン株式会社 糖化ヘモグロビンオキシダーゼ改変体及び測定方法
JP6818718B2 (ja) * 2018-06-28 2021-01-20 キッコーマン株式会社 改変アマドリアーゼ
JPWO2023145689A1 (https=) 2022-01-27 2023-08-03

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EP2602318A4 (en) 2014-02-26
JP6176922B2 (ja) 2017-08-09
EP2602318B1 (en) 2015-09-30
WO2012018094A1 (ja) 2012-02-09
JP6538101B2 (ja) 2019-07-03
EP2602318A1 (en) 2013-06-12
KR102070990B1 (ko) 2020-01-29
US20130267007A1 (en) 2013-10-10
JP2017121261A (ja) 2017-07-13
JPWO2012018094A1 (ja) 2013-10-03
KR20130105614A (ko) 2013-09-25
CN103080308A (zh) 2013-05-01
US9062286B2 (en) 2015-06-23

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