KR102070990B1 - 기질 특이성이 개변된 아마드리아제 - Google Patents

기질 특이성이 개변된 아마드리아제 Download PDF

Info

Publication number
KR102070990B1
KR102070990B1 KR1020137005355A KR20137005355A KR102070990B1 KR 102070990 B1 KR102070990 B1 KR 102070990B1 KR 1020137005355 A KR1020137005355 A KR 1020137005355A KR 20137005355 A KR20137005355 A KR 20137005355A KR 102070990 B1 KR102070990 B1 KR 102070990B1
Authority
KR
South Korea
Prior art keywords
amino acid
seq
substitution
dna
position corresponding
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
KR1020137005355A
Other languages
English (en)
Korean (ko)
Other versions
KR20130105614A (ko
Inventor
아츠시 이치야나기
코조 히로카와
야스코 타나베
요스케 마사카리
Original Assignee
기꼬만 가부시키가이샤
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 기꼬만 가부시키가이샤 filed Critical 기꼬만 가부시키가이샤
Publication of KR20130105614A publication Critical patent/KR20130105614A/ko
Application granted granted Critical
Publication of KR102070990B1 publication Critical patent/KR102070990B1/ko
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0006Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0012Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0026Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5)
    • C12N9/0032Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5) with oxygen as acceptor (1.5.3)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52Genes encoding for enzymes or proenzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/72Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood pigments, e.g. haemoglobin, bilirubin or other porphyrins; involving occult blood
    • G01N33/721Haemoglobin
    • G01N33/723Glycosylated haemoglobin
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90Enzymes; Proenzymes
    • G01N2333/902Oxidoreductases (1.)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Hematology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
KR1020137005355A 2010-08-06 2011-08-04 기질 특이성이 개변된 아마드리아제 Active KR102070990B1 (ko)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
JP2010176967 2010-08-06
JPJP-P-2010-176967 2010-08-06
JPJP-P-2010-213070 2010-09-24
JP2010213070 2010-09-24
PCT/JP2011/067898 WO2012018094A1 (ja) 2010-08-06 2011-08-04 基質特異性が改変されたアマドリアーゼ

Publications (2)

Publication Number Publication Date
KR20130105614A KR20130105614A (ko) 2013-09-25
KR102070990B1 true KR102070990B1 (ko) 2020-01-29

Family

ID=45559590

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020137005355A Active KR102070990B1 (ko) 2010-08-06 2011-08-04 기질 특이성이 개변된 아마드리아제

Country Status (6)

Country Link
US (1) US9062286B2 (https=)
EP (1) EP2602318B1 (https=)
JP (2) JP6176922B2 (https=)
KR (1) KR102070990B1 (https=)
CN (1) CN103080308B (https=)
WO (1) WO2012018094A1 (https=)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012018094A1 (ja) * 2010-08-06 2012-02-09 キッコーマン株式会社 基質特異性が改変されたアマドリアーゼ
JP6282115B2 (ja) 2011-12-28 2018-02-21 キッコーマン株式会社 熱安定性が向上したアマドリアーゼ、その遺伝子および組換えdnaならびに熱安定性が向上したアマドリアーゼの製造法
JP6068916B2 (ja) * 2012-02-02 2017-01-25 キッコーマン株式会社 糖化ヘモグロビン測定用試薬組成物および糖化ヘモグロビン測定方法
WO2013162035A1 (ja) 2012-04-27 2013-10-31 キッコーマン株式会社 フルクトシルヘキサペプチドに作用する改変型アマドリアーゼ
CN103937763B (zh) * 2013-01-22 2016-01-06 清华大学 R112w型酮胺氧化酶及其编码基因和应用
JP6363327B2 (ja) * 2013-03-25 2018-07-25 キッコーマン株式会社 改変アマドリアーゼ
ES2848705T3 (es) 2013-08-09 2021-08-11 Kikkoman Corp Amadoriasa modificada y método para producirla, agente para mejorar la resistencia a tensioactivos de la amadoriasa y composición para medir la HbA1c usando la misma
JP6542671B2 (ja) * 2013-10-25 2019-07-10 キッコーマン株式会社 ヘモグロビンA1cの測定方法および測定キット
CN105683390B (zh) * 2013-10-25 2021-02-26 龟甲万株式会社 使用作用于糖基化肽的阿马多里酶的HbA1c测定方法
JP6749745B2 (ja) * 2014-02-27 2020-09-02 キッコーマン株式会社 アマドリアーゼ含有組成物の安定化方法、熱安定性が向上したアマドリアーゼ含有組成物及びこれを用いた糖化ヘモグロビン測定用組成物
EP3786291B1 (en) 2014-10-24 2023-10-18 Kikkoman Corporation Amadoriase having enhanced dehydrogenase activity
WO2016072520A1 (ja) * 2014-11-07 2016-05-12 キッコーマン株式会社 アニオン性界面活性剤耐性が向上したアマドリアーゼ
CN107002074B (zh) * 2015-06-10 2021-08-10 公立大学法人富山县立大学 活性型突变酶的制造方法及活性型突变酶、以及可溶性化突变蛋白的制造方法
KR102596402B1 (ko) * 2016-04-22 2023-10-31 기꼬만 가부시키가이샤 HbA1c 디히드로게나아제
TWI668229B (zh) 2016-12-13 2019-08-11 財團法人工業技術研究院 重組蛋白質及其製造方法與應用
EP3660156A4 (en) * 2017-07-24 2021-03-31 Riken DECARBOXYLASE AND METHOD FOR PRODUCING UNSATURATED HYDROCARBONS USING THE SAME
JP7157063B2 (ja) 2017-08-31 2022-10-19 キッコーマン株式会社 糖化ヘモグロビンオキシダーゼ改変体及び測定方法
JP6818718B2 (ja) * 2018-06-28 2021-01-20 キッコーマン株式会社 改変アマドリアーゼ
JPWO2023145689A1 (https=) 2022-01-27 2023-08-03

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2010148358A (ja) 2008-12-23 2010-07-08 Toyobo Co Ltd フルクトシル−l−バリルヒスチジン測定用酵素、およびその利用法

Family Cites Families (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS61280297A (ja) 1985-06-04 1986-12-10 Noda Sangyo Kagaku Kenkyusho アマドリ化合物の定量法及びその定量用試薬
JPH0533997A (ja) 1991-07-26 1993-02-09 Nippondenso Co Ltd フイルムドアユニツト
GB9116315D0 (en) 1991-07-29 1991-09-11 Genzyme Ltd Assay
WO1997013872A1 (en) 1995-10-12 1997-04-17 Kyoto Daiichi Kagaku Co., Ltd. Method and assaying amodori compounds
JP3786966B2 (ja) 1995-11-30 2006-06-21 アークレイ株式会社 フルクトシルアミノ酸オキシダーゼ、その製造方法、及び該酵素を用いたアマドリ化合物の測定方法
JP3949854B2 (ja) 1999-10-01 2007-07-25 キッコーマン株式会社 糖化蛋白質の測定方法
JP4231668B2 (ja) 2001-09-04 2009-03-04 キッコーマン株式会社 新規なフルクトシルペプチドオキシダーゼ
CN1701118A (zh) * 2002-09-24 2005-11-23 爱科来株式会社 果糖基胺氧化酶
JP4227820B2 (ja) 2003-03-12 2009-02-18 旭化成ファーマ株式会社 新規な酵素
JP4248900B2 (ja) 2003-03-14 2009-04-02 イチビキ株式会社 新規なフルクトシルアミンオキシダーゼをコードする遺伝子及びそれを用いての該フルクトシルアミンオキシダーゼの製造方法
WO2004104203A1 (ja) 2003-05-21 2004-12-02 Asahi Kasei Pharma Corporation ヘモグロビンA1c測定法およびそれに用いる酵素とその製造法
ES2363228T3 (es) 2003-11-19 2011-07-27 Sekisui Medical Co., Ltd. Procedimiento de ensayo de proteína glicosilada.
JP5074386B2 (ja) 2006-04-25 2012-11-14 キッコーマン株式会社 熱安定性に優れた真核型アマドリアーゼ、その遺伝子及び組換え体dna、並びに熱安定性に優れた真核型アマドリアーゼの製造法
JP5243878B2 (ja) 2008-08-04 2013-07-24 東洋紡株式会社 フルクトシルバリルヒスチジン測定用酵素、およびその利用法
JP5350762B2 (ja) 2008-08-04 2013-11-27 東洋紡株式会社 フルクトシルアミノ酸オキシダーゼ、およびその利用法
KR20110069138A (ko) * 2008-10-09 2011-06-22 교와 메덱스 가부시키가이샤 신규 프럭토실펩티드옥시다아제
CN102171338B (zh) * 2008-10-10 2014-08-13 东洋纺织株式会社 具有果糖基缬氨酰基组氨酸氧化酶活性的蛋白质及其修饰产物以及其应用
JP5476021B2 (ja) * 2008-10-17 2014-04-23 東洋紡株式会社 フルクトシルアミノ酸オキシダーゼ改変体およびその利用
JP5465415B2 (ja) 2008-10-29 2014-04-09 東洋紡株式会社 フルクトシルアミノ酸測定用酵素、およびその利用法
JP2010176967A (ja) 2009-01-28 2010-08-12 Fujitsu Component Ltd 電線接続端子並びに電線接続端子を用いた電線接続方法、電線接続構造および電磁継電器
JP5548371B2 (ja) 2009-03-11 2014-07-16 株式会社野村総合研究所 環境情報変更システム、端末装置およびプログラム
JP2010233502A (ja) 2009-03-31 2010-10-21 Toyobo Co Ltd フルクトシルリジンの影響が低減する糖化タンパク質測定用試薬組成物
JP2010233501A (ja) 2009-03-31 2010-10-21 Toyobo Co Ltd フルクトシルバリンの影響が低減する糖化タンパク質測定用試薬組成物
EP2287295A1 (en) * 2009-08-03 2011-02-23 Roche Diagnostics GmbH Mutant Fructosyl amino acid oxidase
EP2281900A1 (en) * 2009-08-03 2011-02-09 Roche Diagnostics GmbH Fructosyl peptidyl oxidase and sensor for assaying a glycated protein
WO2012018094A1 (ja) * 2010-08-06 2012-02-09 キッコーマン株式会社 基質特異性が改変されたアマドリアーゼ

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2010148358A (ja) 2008-12-23 2010-07-08 Toyobo Co Ltd フルクトシル−l−バリルヒスチジン測定用酵素、およびその利用法

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
BBRC, Vol. 311, pp. 104-111 (2003.)
Journal of Diabetes Science and Technology, Vol. 3, pp. 585-592 (2009.05.)
Protein Engineering Design & Selection, Vol. 21, No. 4, pp. 233-239 (2008.01.31.)*

Also Published As

Publication number Publication date
EP2602318A4 (en) 2014-02-26
JP6176922B2 (ja) 2017-08-09
EP2602318B1 (en) 2015-09-30
WO2012018094A1 (ja) 2012-02-09
JP6538101B2 (ja) 2019-07-03
EP2602318A1 (en) 2013-06-12
CN103080308B (zh) 2015-08-05
US20130267007A1 (en) 2013-10-10
JP2017121261A (ja) 2017-07-13
JPWO2012018094A1 (ja) 2013-10-03
KR20130105614A (ko) 2013-09-25
CN103080308A (zh) 2013-05-01
US9062286B2 (en) 2015-06-23

Similar Documents

Publication Publication Date Title
KR102070990B1 (ko) 기질 특이성이 개변된 아마드리아제
JP6726243B2 (ja) フルクトシルヘキサペプチドに作用する改変型アマドリアーゼ
US8828699B2 (en) Eukaryotic amadoriase, gene and recombinant DNA for the eukaryotic amadoriase, and process for production of the eukaryotic amadoriase
JP5442432B2 (ja) 糖化ヘキサペプチドの測定方法
JP2020141690A (ja) 糖化ペプチドに作用するアマドリアーゼを用いたHbA1c測定法
US11499143B2 (en) Amadoriase having enhanced dehydrogenase activity
JP6068916B2 (ja) 糖化ヘモグロビン測定用試薬組成物および糖化ヘモグロビン測定方法
JP6843740B2 (ja) 比活性が向上したアマドリアーゼ
JP7157063B2 (ja) 糖化ヘモグロビンオキシダーゼ改変体及び測定方法
US9708586B2 (en) Amadoriase having altered substrate specificity
WO2012043601A1 (ja) アマドリアーゼ改変体

Legal Events

Date Code Title Description
PA0105 International application

St.27 status event code: A-0-1-A10-A15-nap-PA0105

PG1501 Laying open of application

St.27 status event code: A-1-1-Q10-Q12-nap-PG1501

A201 Request for examination
PA0201 Request for examination

St.27 status event code: A-1-2-D10-D11-exm-PA0201

E902 Notification of reason for refusal
PE0902 Notice of grounds for rejection

St.27 status event code: A-1-2-D10-D21-exm-PE0902

T11-X000 Administrative time limit extension requested

St.27 status event code: U-3-3-T10-T11-oth-X000

AMND Amendment
E13-X000 Pre-grant limitation requested

St.27 status event code: A-2-3-E10-E13-lim-X000

P11-X000 Amendment of application requested

St.27 status event code: A-2-2-P10-P11-nap-X000

P13-X000 Application amended

St.27 status event code: A-2-2-P10-P13-nap-X000

E902 Notification of reason for refusal
PE0902 Notice of grounds for rejection

St.27 status event code: A-1-2-D10-D21-exm-PE0902

T11-X000 Administrative time limit extension requested

St.27 status event code: U-3-3-T10-T11-oth-X000

AMND Amendment
P11-X000 Amendment of application requested

St.27 status event code: A-2-2-P10-P11-nap-X000

P13-X000 Application amended

St.27 status event code: A-2-2-P10-P13-nap-X000

E601 Decision to refuse application
PE0601 Decision on rejection of patent

St.27 status event code: N-2-6-B10-B15-exm-PE0601

X091 Application refused [patent]
T11-X000 Administrative time limit extension requested

St.27 status event code: U-3-3-T10-T11-oth-X000

T13-X000 Administrative time limit extension granted

St.27 status event code: U-3-3-T10-T13-oth-X000

T11-X000 Administrative time limit extension requested

St.27 status event code: U-3-3-T10-T11-oth-X000

T13-X000 Administrative time limit extension granted

St.27 status event code: U-3-3-T10-T13-oth-X000

AMND Amendment
P11-X000 Amendment of application requested

St.27 status event code: A-2-2-P10-P11-nap-X000

P13-X000 Application amended

St.27 status event code: A-2-2-P10-P13-nap-X000

PX0901 Re-examination

St.27 status event code: A-2-3-E10-E12-rex-PX0901

PX0701 Decision of registration after re-examination

St.27 status event code: A-3-4-F10-F13-rex-PX0701

X701 Decision to grant (after re-examination)
GRNT Written decision to grant
PR0701 Registration of establishment

St.27 status event code: A-2-4-F10-F11-exm-PR0701

PR1002 Payment of registration fee

St.27 status event code: A-2-2-U10-U12-oth-PR1002

Fee payment year number: 1

PG1601 Publication of registration

St.27 status event code: A-4-4-Q10-Q13-nap-PG1601

PR1001 Payment of annual fee

St.27 status event code: A-4-4-U10-U11-oth-PR1001

Fee payment year number: 4

PR1001 Payment of annual fee

St.27 status event code: A-4-4-U10-U11-oth-PR1001

Fee payment year number: 5

PR1001 Payment of annual fee

St.27 status event code: A-4-4-U10-U11-oth-PR1001

Fee payment year number: 6

PR1001 Payment of annual fee

St.27 status event code: A-4-4-U10-U11-oth-PR1001

Fee payment year number: 7

U11 Full renewal or maintenance fee paid

Free format text: ST27 STATUS EVENT CODE: A-4-4-U10-U11-OTH-PR1001 (AS PROVIDED BY THE NATIONAL OFFICE)

Year of fee payment: 7