CN102687020A - 利用特征肽和质谱对混合物中的单个重组蛋白进行多重定量 - Google Patents

利用特征肽和质谱对混合物中的单个重组蛋白进行多重定量 Download PDF

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CN102687020A
CN102687020A CN2010800562278A CN201080056227A CN102687020A CN 102687020 A CN102687020 A CN 102687020A CN 2010800562278 A CN2010800562278 A CN 2010800562278A CN 201080056227 A CN201080056227 A CN 201080056227A CN 102687020 A CN102687020 A CN 102687020A
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described method
peptide
recombinant
polyclonal antibody
antibody
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T.弗兰德森
H.内斯泰德
J.W.森
P.F.詹森
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Symphogen AS
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/62—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating the ionisation of gases, e.g. aerosols; by investigating electric discharges, e.g. emission of cathode
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • G01N33/6851—Methods of protein analysis involving laser desorption ionisation mass spectrometry

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
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  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
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  • Urology & Nephrology (AREA)
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  • Pathology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Food Science & Technology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Optics & Photonics (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
CN2010800562278A 2009-10-09 2010-10-07 利用特征肽和质谱对混合物中的单个重组蛋白进行多重定量 Pending CN102687020A (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
DKPA200901112 2009-10-09
DKPA200901112 2009-10-09
US25623209P 2009-10-29 2009-10-29
US61/256,232 2009-10-29
PCT/DK2010/050258 WO2011042027A2 (en) 2009-10-09 2010-10-07 Multiplex quantitation of individual recombinant proteins in a mixture by signature peptides and mass spectrometry

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CN102687020A true CN102687020A (zh) 2012-09-19

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CN2010800562278A Pending CN102687020A (zh) 2009-10-09 2010-10-07 利用特征肽和质谱对混合物中的单个重组蛋白进行多重定量

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US (1) US20120264155A1 (https=)
EP (1) EP2486411A2 (https=)
JP (1) JP2013507603A (https=)
KR (1) KR20120100982A (https=)
CN (1) CN102687020A (https=)
AU (1) AU2010305150A1 (https=)
BR (1) BR112012007815A2 (https=)
CA (1) CA2776508A1 (https=)
IL (1) IL218821A0 (https=)
IN (1) IN2012DN03911A (https=)
MX (1) MX2012003538A (https=)
RU (1) RU2012118620A (https=)
WO (1) WO2011042027A2 (https=)
ZA (1) ZA201202431B (https=)

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CN105218671A (zh) * 2015-09-29 2016-01-06 李艳华 一种传染病病原体全人源化抗体的制备方法
CN107525934A (zh) * 2016-06-21 2017-12-29 张效龙 一种测定血液中抗体氨基酸序列的方法
CN108072728A (zh) * 2016-11-16 2018-05-25 中国科学院大连化学物理研究所 一种基于数据依赖性质谱扫描模式的谱图库建立方法及其应用
CN108169397A (zh) * 2017-11-30 2018-06-15 中国农业科学院北京畜牧兽医研究所 一种相对定量分析肉鸡谷胱甘肽s转移酶gsta2的方法
CN108226516A (zh) * 2017-11-30 2018-06-29 中国农业科学院北京畜牧兽医研究所 一种相对定量分析肉鸡脂肪酸去饱和酶fads1的方法
CN108956789A (zh) * 2017-05-17 2018-12-07 中国科学院大连化学物理研究所 一种血清中免疫球蛋白g糖肽的绝对定量分析方法
CN109900815A (zh) * 2017-12-08 2019-06-18 中国科学院大连化学物理研究所 血清中IgG糖肽的绝对定量分析
CN111551739A (zh) * 2020-04-27 2020-08-18 杭州璞湃科技有限公司 一种免疫球蛋白a和免疫球蛋白a1的检测方法及其试剂盒
CN111766323A (zh) * 2020-07-10 2020-10-13 中国检验检疫科学研究院 一种用于检测骆驼奶中掺入牛奶的特征肽组合及方法
CN113848258A (zh) * 2020-06-25 2021-12-28 希森美康株式会社 Aβ肽的测定方法及在该方法中使用的试剂组合物
CN113933409A (zh) * 2021-09-14 2022-01-14 上海中科新生命生物科技有限公司 基于液相色谱-质谱检测血清中特瑞普利单抗的方法
CN118221778A (zh) * 2024-04-25 2024-06-21 烟台大学 拟穴青蟹Scyreprocin蛋白的特征肽段及其在雌雄鉴别方面的应用

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US8679767B2 (en) 2011-05-12 2014-03-25 Genentech, Inc. Multiple reaction monitoring LC-MS/MS method to detect therapeutic antibodies in animal samples using framework signature peptides
WO2013019714A1 (en) 2011-07-29 2013-02-07 The Trustees Of Columbia University In The City Of New York Mems affinity sensor for continuous monitoring of analytes
WO2016022696A1 (en) * 2014-08-05 2016-02-11 The Trustees Of Columbia University In The City Of New York Method of isolating aptamers for minimal residual disease detection
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CN105242050A (zh) * 2015-09-16 2016-01-13 同济大学 一种不同生理或病理条件下整体蛋白质的定量分析方法
GB201603291D0 (en) 2016-02-25 2016-04-13 Binding Site Group The Ltd Antibodies
CN108700598A (zh) * 2016-03-25 2018-10-23 豪夫迈·罗氏有限公司 多路总抗体和抗体缀合的药物量化测定法
EP3465221B1 (en) 2016-05-27 2020-07-22 H. Hoffnabb-La Roche Ag Bioanalytical method for the characterization of site-specific antibody-drug conjugates
WO2018035350A1 (en) * 2016-08-17 2018-02-22 Momenta Pharmaceuticals, Inc. Glycan oxonium ion profiling of glycosylated proteins
ES2978396T3 (es) * 2017-08-01 2024-09-11 Amgen Inc Sistemas y métodos para la preparación en tiempo real de una muestra de polipéptidos para el análisis por espectrometría de masas
US10615016B2 (en) * 2017-09-07 2020-04-07 Thermo Fisher Scientific (Bremen) Gmbh Determining isotope ratios using mass spectrometry
EP3746472A2 (en) * 2018-01-31 2020-12-09 Regeneron Pharmaceuticals, Inc. Glucuronylation as acidic post-translational modification on therapeutic monoclonal antibodies
JP7205077B2 (ja) * 2018-05-10 2023-01-17 株式会社島津製作所 分析方法、処理装置、分析装置およびプログラム
CN110824096B (zh) * 2018-08-13 2022-11-29 齐鲁制药有限公司 一种蛋白错配二硫键的分析方法
CN110007019A (zh) * 2019-03-29 2019-07-12 绿城农科检测技术有限公司 一种同时定量检测母乳中三种抗体含量的方法
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JP7778705B2 (ja) 2020-02-21 2025-12-02 ジェンマブ ビー．ブイ． 糖蛋白質の定量のための方法
US20230228763A1 (en) * 2020-06-18 2023-07-20 Northwestern University Process for direct readout of immunoglobulins
DE102022115561B4 (de) * 2022-06-22 2024-06-13 Bruker Daltonics GmbH & Co. KG Massenspektrometrische Bestimmung der Zelltoxizität
CN120399082B (zh) * 2025-07-04 2025-08-26 北京溯本源和生物科技有限公司 一种用于检测猫Cys-C蛋白的单克隆抗体组合及应用

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Cited By (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105218671A (zh) * 2015-09-29 2016-01-06 李艳华 一种传染病病原体全人源化抗体的制备方法
CN107525934A (zh) * 2016-06-21 2017-12-29 张效龙 一种测定血液中抗体氨基酸序列的方法
CN108072728A (zh) * 2016-11-16 2018-05-25 中国科学院大连化学物理研究所 一种基于数据依赖性质谱扫描模式的谱图库建立方法及其应用
CN108956789A (zh) * 2017-05-17 2018-12-07 中国科学院大连化学物理研究所 一种血清中免疫球蛋白g糖肽的绝对定量分析方法
CN108169397A (zh) * 2017-11-30 2018-06-15 中国农业科学院北京畜牧兽医研究所 一种相对定量分析肉鸡谷胱甘肽s转移酶gsta2的方法
CN108226516A (zh) * 2017-11-30 2018-06-29 中国农业科学院北京畜牧兽医研究所 一种相对定量分析肉鸡脂肪酸去饱和酶fads1的方法
CN108169397B (zh) * 2017-11-30 2019-11-15 中国农业科学院北京畜牧兽医研究所 一种相对定量分析肉鸡谷胱甘肽s转移酶gsta2的方法
CN109900815B (zh) * 2017-12-08 2021-06-29 中国科学院大连化学物理研究所 血清中IgG糖肽的绝对定量分析
CN109900815A (zh) * 2017-12-08 2019-06-18 中国科学院大连化学物理研究所 血清中IgG糖肽的绝对定量分析
CN111551739A (zh) * 2020-04-27 2020-08-18 杭州璞湃科技有限公司 一种免疫球蛋白a和免疫球蛋白a1的检测方法及其试剂盒
CN113848258A (zh) * 2020-06-25 2021-12-28 希森美康株式会社 Aβ肽的测定方法及在该方法中使用的试剂组合物
US12411146B2 (en) 2020-06-25 2025-09-09 Sysmex Corporation Method for measuring amyloid β peptide
CN111766323A (zh) * 2020-07-10 2020-10-13 中国检验检疫科学研究院 一种用于检测骆驼奶中掺入牛奶的特征肽组合及方法
CN113933409A (zh) * 2021-09-14 2022-01-14 上海中科新生命生物科技有限公司 基于液相色谱-质谱检测血清中特瑞普利单抗的方法
CN113933409B (zh) * 2021-09-14 2023-11-14 上海中科新生命生物科技有限公司 基于液相色谱-质谱检测血清中特瑞普利单抗的方法
CN118221778A (zh) * 2024-04-25 2024-06-21 烟台大学 拟穴青蟹Scyreprocin蛋白的特征肽段及其在雌雄鉴别方面的应用

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MX2012003538A (es) 2012-08-03
KR20120100982A (ko) 2012-09-12
BR112012007815A2 (pt) 2018-03-20
AU2010305150A1 (en) 2012-04-05
WO2011042027A2 (en) 2011-04-14
RU2012118620A (ru) 2013-11-20
EP2486411A2 (en) 2012-08-15
IL218821A0 (en) 2012-06-28
IN2012DN03911A (https=) 2015-09-04
US20120264155A1 (en) 2012-10-18
CA2776508A1 (en) 2011-04-14
JP2013507603A (ja) 2013-03-04
ZA201202431B (en) 2012-12-27
WO2011042027A3 (en) 2011-09-22

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