BR112012007815A2 - quantificação multiplex de proteínas recombinantes em uma mistura por peptídeos de assinatura e espectrometria de massa. - Google Patents
quantificação multiplex de proteínas recombinantes em uma mistura por peptídeos de assinatura e espectrometria de massa.Info
- Publication number
- BR112012007815A2 BR112012007815A2 BR112012007815A BR112012007815A BR112012007815A2 BR 112012007815 A2 BR112012007815 A2 BR 112012007815A2 BR 112012007815 A BR112012007815 A BR 112012007815A BR 112012007815 A BR112012007815 A BR 112012007815A BR 112012007815 A2 BR112012007815 A2 BR 112012007815A2
- Authority
- BR
- Brazil
- Prior art keywords
- recombinant proteins
- mixture
- mass spectrometry
- signature peptides
- multiplex
- Prior art date
Links
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 title abstract 3
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 title abstract 3
- 238000004949 mass spectrometry Methods 0.000 title abstract 2
- 239000000203 mixture Substances 0.000 title abstract 2
- 102000004196 processed proteins & peptides Human genes 0.000 title abstract 2
- 108090000765 processed proteins & peptides Proteins 0.000 title abstract 2
- 238000011002 quantification Methods 0.000 title 1
- 238000004458 analytical method Methods 0.000 abstract 1
- 239000012228 culture supernatant Substances 0.000 abstract 1
- 239000011159 matrix material Substances 0.000 abstract 1
- 210000002966 serum Anatomy 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/62—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating the ionisation of gases, e.g. aerosols; by investigating electric discharges, e.g. emission of cathode
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6848—Methods of protein analysis involving mass spectrometry
- G01N33/6851—Methods of protein analysis involving laser desorption ionisation mass spectrometry
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Bioinformatics & Computational Biology (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Food Science & Technology (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Optics & Photonics (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- Peptides Or Proteins (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DKPA200901112 | 2009-10-09 | ||
| US25623209P | 2009-10-29 | 2009-10-29 | |
| PCT/DK2010/050258 WO2011042027A2 (en) | 2009-10-09 | 2010-10-07 | Multiplex quantitation of individual recombinant proteins in a mixture by signature peptides and mass spectrometry |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BR112012007815A2 true BR112012007815A2 (pt) | 2018-03-20 |
Family
ID=42166454
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR112012007815A BR112012007815A2 (pt) | 2009-10-09 | 2010-10-07 | quantificação multiplex de proteínas recombinantes em uma mistura por peptídeos de assinatura e espectrometria de massa. |
Country Status (14)
| Country | Link |
|---|---|
| US (1) | US20120264155A1 (https=) |
| EP (1) | EP2486411A2 (https=) |
| JP (1) | JP2013507603A (https=) |
| KR (1) | KR20120100982A (https=) |
| CN (1) | CN102687020A (https=) |
| AU (1) | AU2010305150A1 (https=) |
| BR (1) | BR112012007815A2 (https=) |
| CA (1) | CA2776508A1 (https=) |
| IL (1) | IL218821A0 (https=) |
| IN (1) | IN2012DN03911A (https=) |
| MX (1) | MX2012003538A (https=) |
| RU (1) | RU2012118620A (https=) |
| WO (1) | WO2011042027A2 (https=) |
| ZA (1) | ZA201202431B (https=) |
Families Citing this family (34)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2241875A1 (en) * | 2009-04-14 | 2010-10-20 | Koninklijke Philips Electronics N.V. | Up-concentration of organic microobjects for microscopic imaging. |
| US8679767B2 (en) | 2011-05-12 | 2014-03-25 | Genentech, Inc. | Multiple reaction monitoring LC-MS/MS method to detect therapeutic antibodies in animal samples using framework signature peptides |
| WO2013019714A1 (en) | 2011-07-29 | 2013-02-07 | The Trustees Of Columbia University In The City Of New York | Mems affinity sensor for continuous monitoring of analytes |
| WO2016022696A1 (en) * | 2014-08-05 | 2016-02-11 | The Trustees Of Columbia University In The City Of New York | Method of isolating aptamers for minimal residual disease detection |
| WO2016145390A1 (en) | 2015-03-12 | 2016-09-15 | Mars, Incorporated | Ultra high resolution mass spectrometry and methods of using the same |
| US10794916B2 (en) | 2015-05-06 | 2020-10-06 | Momenta Pharmaceuticals, Inc. | Methods of glycoprotein analysis |
| CN105242050A (zh) * | 2015-09-16 | 2016-01-13 | 同济大学 | 一种不同生理或病理条件下整体蛋白质的定量分析方法 |
| CN105218671A (zh) * | 2015-09-29 | 2016-01-06 | 李艳华 | 一种传染病病原体全人源化抗体的制备方法 |
| GB201603291D0 (en) | 2016-02-25 | 2016-04-13 | Binding Site Group The Ltd | Antibodies |
| CN108700598A (zh) * | 2016-03-25 | 2018-10-23 | 豪夫迈·罗氏有限公司 | 多路总抗体和抗体缀合的药物量化测定法 |
| EP3465221B1 (en) | 2016-05-27 | 2020-07-22 | H. Hoffnabb-La Roche Ag | Bioanalytical method for the characterization of site-specific antibody-drug conjugates |
| CN107525934A (zh) * | 2016-06-21 | 2017-12-29 | 张效龙 | 一种测定血液中抗体氨基酸序列的方法 |
| WO2018035350A1 (en) * | 2016-08-17 | 2018-02-22 | Momenta Pharmaceuticals, Inc. | Glycan oxonium ion profiling of glycosylated proteins |
| CN108072728A (zh) * | 2016-11-16 | 2018-05-25 | 中国科学院大连化学物理研究所 | 一种基于数据依赖性质谱扫描模式的谱图库建立方法及其应用 |
| CN108956789A (zh) * | 2017-05-17 | 2018-12-07 | 中国科学院大连化学物理研究所 | 一种血清中免疫球蛋白g糖肽的绝对定量分析方法 |
| ES2978396T3 (es) * | 2017-08-01 | 2024-09-11 | Amgen Inc | Sistemas y métodos para la preparación en tiempo real de una muestra de polipéptidos para el análisis por espectrometría de masas |
| US10615016B2 (en) * | 2017-09-07 | 2020-04-07 | Thermo Fisher Scientific (Bremen) Gmbh | Determining isotope ratios using mass spectrometry |
| CN108226516B (zh) * | 2017-11-30 | 2020-05-19 | 中国农业科学院北京畜牧兽医研究所 | 一种相对定量分析肉鸡脂肪酸去饱和酶fads1的方法 |
| CN108169397B (zh) * | 2017-11-30 | 2019-11-15 | 中国农业科学院北京畜牧兽医研究所 | 一种相对定量分析肉鸡谷胱甘肽s转移酶gsta2的方法 |
| CN109900815B (zh) * | 2017-12-08 | 2021-06-29 | 中国科学院大连化学物理研究所 | 血清中IgG糖肽的绝对定量分析 |
| EP3746472A2 (en) * | 2018-01-31 | 2020-12-09 | Regeneron Pharmaceuticals, Inc. | Glucuronylation as acidic post-translational modification on therapeutic monoclonal antibodies |
| JP7205077B2 (ja) * | 2018-05-10 | 2023-01-17 | 株式会社島津製作所 | 分析方法、処理装置、分析装置およびプログラム |
| CN110824096B (zh) * | 2018-08-13 | 2022-11-29 | 齐鲁制药有限公司 | 一种蛋白错配二硫键的分析方法 |
| CN110007019A (zh) * | 2019-03-29 | 2019-07-12 | 绿城农科检测技术有限公司 | 一种同时定量检测母乳中三种抗体含量的方法 |
| GB201910697D0 (en) * | 2019-07-26 | 2019-09-11 | Binding Site Group Ltd | Lonisation control |
| JP7778705B2 (ja) | 2020-02-21 | 2025-12-02 | ジェンマブ ビー．ブイ． | 糖蛋白質の定量のための方法 |
| CN111551739A (zh) * | 2020-04-27 | 2020-08-18 | 杭州璞湃科技有限公司 | 一种免疫球蛋白a和免疫球蛋白a1的检测方法及其试剂盒 |
| US20230228763A1 (en) * | 2020-06-18 | 2023-07-20 | Northwestern University | Process for direct readout of immunoglobulins |
| JP7478040B2 (ja) | 2020-06-25 | 2024-05-02 | シスメックス株式会社 | Ａβペプチドの測定方法及びその方法に用いられる試薬組成物 |
| CN111766323B (zh) * | 2020-07-10 | 2022-06-14 | 中国检验检疫科学研究院 | 一种用于检测骆驼奶中掺入牛奶的特征肽组合及方法 |
| CN113933409B (zh) * | 2021-09-14 | 2023-11-14 | 上海中科新生命生物科技有限公司 | 基于液相色谱-质谱检测血清中特瑞普利单抗的方法 |
| DE102022115561B4 (de) * | 2022-06-22 | 2024-06-13 | Bruker Daltonics GmbH & Co. KG | Massenspektrometrische Bestimmung der Zelltoxizität |
| CN118221778A (zh) * | 2024-04-25 | 2024-06-21 | 烟台大学 | 拟穴青蟹Scyreprocin蛋白的特征肽段及其在雌雄鉴别方面的应用 |
| CN120399082B (zh) * | 2025-07-04 | 2025-08-26 | 北京溯本源和生物科技有限公司 | 一种用于检测猫Cys-C蛋白的单克隆抗体组合及应用 |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003087834A2 (en) * | 2002-04-08 | 2003-10-23 | Affinium Pharmaceuticals, Inc. | High throughput analysis of recombinant proteins in multi-well plates |
| EP2186879B1 (en) | 2002-10-03 | 2016-11-23 | Norman Leigh Anderson | High sensitivity quantitation of peptides by mass spectrometry |
| US20060154318A1 (en) * | 2004-06-09 | 2006-07-13 | Anderson Norman L | Stable isotope labeled polypeptide standards for protein quantitation |
| US8288109B2 (en) * | 2004-07-20 | 2012-10-16 | Symphogen A/S | Procedure for structural characterization of a recombinant polyclonal protein or a polyclonal cell line |
| EA014182B1 (ru) | 2004-07-20 | 2010-10-29 | Симфоген А/С | КОМПОЗИЦИЯ ПОЛИКЛОНАЛЬНЫХ АНТИ-RhD АНТИТЕЛ, СПОСОБ ЕЁ ПОЛУЧЕНИЯ И ПРИМЕНЕНИЕ КОМПОЗИЦИИ |
| US20090298196A1 (en) * | 2005-01-28 | 2009-12-03 | Uwabe Ken-Ichiro | Quantitative measurement method for recombinant protein |
| JP2006300758A (ja) * | 2005-04-21 | 2006-11-02 | Apro Life Science Institute Inc | 内部標準ペプチドを用いて生体由来試料に含まれる標的タンパク質を定量する方法 |
| US7850965B2 (en) | 2005-12-05 | 2010-12-14 | Symphogen A/S | Anti-orthopoxvirus recombinant polyclonal antibody |
| WO2007101441A1 (en) | 2006-03-06 | 2007-09-13 | Symphogen A/S | Recombinant polyclonal antibody for treatment of respiratory syncytial virus infections |
| BRPI0808551B1 (pt) * | 2007-03-01 | 2022-04-05 | Symphogen A/S | Composições de anticorpo para o receptor do fator de crescimento antiepidérmico recombinante, molécula de ligação bi-específica e composição farmacêutica que os compreende |
| JP2010534057A (ja) | 2007-03-06 | 2010-11-04 | シムフォゲン・アクティーゼルスカブ | 呼吸器合胞体ウイルス感染症を治療するための組換え抗体 |
| US20090000419A1 (en) * | 2007-06-29 | 2009-01-01 | Harley-Davidson Motor Company Group, Inc. | Handlebar assembly with axial locking feature |
-
2010
- 2010-10-07 MX MX2012003538A patent/MX2012003538A/es not_active Application Discontinuation
- 2010-10-07 JP JP2012532458A patent/JP2013507603A/ja active Pending
- 2010-10-07 CN CN2010800562278A patent/CN102687020A/zh active Pending
- 2010-10-07 BR BR112012007815A patent/BR112012007815A2/pt not_active IP Right Cessation
- 2010-10-07 CA CA2776508A patent/CA2776508A1/en not_active Abandoned
- 2010-10-07 WO PCT/DK2010/050258 patent/WO2011042027A2/en not_active Ceased
- 2010-10-07 AU AU2010305150A patent/AU2010305150A1/en not_active Abandoned
- 2010-10-07 US US13/501,051 patent/US20120264155A1/en not_active Abandoned
- 2010-10-07 KR KR1020127011934A patent/KR20120100982A/ko not_active Withdrawn
- 2010-10-07 RU RU2012118620/15A patent/RU2012118620A/ru unknown
- 2010-10-07 IN IN3911DEN2012 patent/IN2012DN03911A/en unknown
- 2010-10-07 EP EP10774121A patent/EP2486411A2/en not_active Withdrawn
-
2012
- 2012-03-25 IL IL218821A patent/IL218821A0/en unknown
- 2012-04-03 ZA ZA2012/02431A patent/ZA201202431B/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| MX2012003538A (es) | 2012-08-03 |
| KR20120100982A (ko) | 2012-09-12 |
| AU2010305150A1 (en) | 2012-04-05 |
| WO2011042027A2 (en) | 2011-04-14 |
| RU2012118620A (ru) | 2013-11-20 |
| EP2486411A2 (en) | 2012-08-15 |
| IL218821A0 (en) | 2012-06-28 |
| CN102687020A (zh) | 2012-09-19 |
| IN2012DN03911A (https=) | 2015-09-04 |
| US20120264155A1 (en) | 2012-10-18 |
| CA2776508A1 (en) | 2011-04-14 |
| JP2013507603A (ja) | 2013-03-04 |
| ZA201202431B (en) | 2012-12-27 |
| WO2011042027A3 (en) | 2011-09-22 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| B08F | Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette] | ||
| B08K | Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette] |
Free format text: EM VIRTUDE DO ARQUIVAMENTO PUBLICADO NA RPI 2465 DE 03-04-2018 E CONSIDERANDO AUSENCIA DE MANIFESTACAO DENTRO DOS PRAZOS LEGAIS, INFORMO QUE CABE SER MANTIDO O ARQUIVAMENTO DO PEDIDO DE PATENTE, CONFORME O DISPOSTO NO ARTIGO 12, DA RESOLUCAO 113/2013. |