ATE517192T1 - Verfahren zur isolierung von zellen - Google Patents

Verfahren zur isolierung von zellen

Info

Publication number
ATE517192T1
ATE517192T1 AT07784617T AT07784617T ATE517192T1 AT E517192 T1 ATE517192 T1 AT E517192T1 AT 07784617 T AT07784617 T AT 07784617T AT 07784617 T AT07784617 T AT 07784617T AT E517192 T1 ATE517192 T1 AT E517192T1
Authority
AT
Austria
Prior art keywords
cells
isolation
isolating
sample
complex sample
Prior art date
Application number
AT07784617T
Other languages
English (en)
Inventor
Peter Rossmanith
Ingeborg Hein
Martin Wagner
Original Assignee
Merck Patent Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Merck Patent Gmbh filed Critical Merck Patent Gmbh
Application granted granted Critical
Publication of ATE517192T1 publication Critical patent/ATE517192T1/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/02Separating microorganisms from their culture media
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/24Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Biomedical Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AT07784617T 2006-08-10 2007-08-10 Verfahren zur isolierung von zellen ATE517192T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
AT13462006 2006-08-10
PCT/AT2007/000388 WO2008017097A1 (en) 2006-08-10 2007-08-10 Method for isolating cells

Publications (1)

Publication Number Publication Date
ATE517192T1 true ATE517192T1 (de) 2011-08-15

Family

ID=38645811

Family Applications (1)

Application Number Title Priority Date Filing Date
AT07784617T ATE517192T1 (de) 2006-08-10 2007-08-10 Verfahren zur isolierung von zellen

Country Status (4)

Country Link
US (1) US9328326B2 (de)
EP (1) EP2049677B1 (de)
AT (1) ATE517192T1 (de)
WO (1) WO2008017097A1 (de)

Families Citing this family (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120122110A1 (en) * 2009-06-18 2012-05-17 Merck Patent Gesellschaft Mit Besdchrankter Haftung Method for isolating cells
EP2266696A1 (de) 2009-06-23 2010-12-29 Veterinärmedizinische Universität Wien Vorrichtung zur Vereinzelung von Mikroobjekten
ES2565335T3 (es) 2009-08-17 2016-04-04 Merck Patent Gmbh Método para aislar virus
EP2325312A1 (de) 2009-11-19 2011-05-25 Qiagen GmbH Verfahren zur selektiven Anreicherung und Isolierung mikrobieller und optional zusätzlich viraler Nukleinsäuren
EP2576599B1 (de) * 2010-06-02 2015-03-18 Merck Patent GmbH Genetisch modifiziertes bakterium der spezies listeria monocytogenes
US10167508B2 (en) 2010-08-06 2019-01-01 Ariosa Diagnostics, Inc. Detection of genetic abnormalities
US11031095B2 (en) 2010-08-06 2021-06-08 Ariosa Diagnostics, Inc. Assay systems for determination of fetal copy number variation
US20130040375A1 (en) 2011-08-08 2013-02-14 Tandem Diagnotics, Inc. Assay systems for genetic analysis
US8700338B2 (en) 2011-01-25 2014-04-15 Ariosa Diagnosis, Inc. Risk calculation for evaluation of fetal aneuploidy
US11203786B2 (en) 2010-08-06 2021-12-21 Ariosa Diagnostics, Inc. Detection of target nucleic acids using hybridization
US20140342940A1 (en) 2011-01-25 2014-11-20 Ariosa Diagnostics, Inc. Detection of Target Nucleic Acids using Hybridization
US20130261003A1 (en) 2010-08-06 2013-10-03 Ariosa Diagnostics, In. Ligation-based detection of genetic variants
US20120034603A1 (en) 2010-08-06 2012-02-09 Tandem Diagnostics, Inc. Ligation-based detection of genetic variants
US10533223B2 (en) 2010-08-06 2020-01-14 Ariosa Diagnostics, Inc. Detection of target nucleic acids using hybridization
US9994897B2 (en) 2013-03-08 2018-06-12 Ariosa Diagnostics, Inc. Non-invasive fetal sex determination
US11270781B2 (en) 2011-01-25 2022-03-08 Ariosa Diagnostics, Inc. Statistical analysis for non-invasive sex chromosome aneuploidy determination
US10131947B2 (en) 2011-01-25 2018-11-20 Ariosa Diagnostics, Inc. Noninvasive detection of fetal aneuploidy in egg donor pregnancies
US8756020B2 (en) 2011-01-25 2014-06-17 Ariosa Diagnostics, Inc. Enhanced risk probabilities using biomolecule estimations
JP6147730B2 (ja) 2011-04-27 2017-06-14 メルク パテント ゲゼルシャフト ミット ベシュレンクテル ハフツングMerck Patent Gesellschaft mit beschraenkter Haftung 細胞を溶解する方法
EP2734280A4 (de) * 2011-07-22 2015-04-15 Bio Merieux Inc Verfahren und kit zur isolierung von mikroorganismen aus kultur
US8712697B2 (en) 2011-09-07 2014-04-29 Ariosa Diagnostics, Inc. Determination of copy number variations using binomial probability calculations
US10289800B2 (en) 2012-05-21 2019-05-14 Ariosa Diagnostics, Inc. Processes for calculating phased fetal genomic sequences
EP2684947A1 (de) 2012-07-09 2014-01-15 Biocartis SA Verfahren zum Filtern einer biologischen Probe
WO2014015269A1 (en) 2012-07-19 2014-01-23 Ariosa Diagnostics, Inc. Multiplexed sequential ligation-based detection of genetic variants
KR101933621B1 (ko) 2012-09-28 2018-12-28 삼성전자주식회사 소포를 분리하기 위한 조성물, 키트 및 이를 이용하여 소포를 분리하는 방법
US10345299B2 (en) * 2013-12-18 2019-07-09 Asahi Kasei Kabushiki Kaisha Method for detecting coliform bacteria contained in milk
US8906972B1 (en) 2013-12-27 2014-12-09 King Fahd University Of Petroleum And Minerals Aqueous two phase (ATPSs) of pH-responsive poly(phosphonate-alt-sulfur dioxide) with PEG
CN106661606B (zh) 2014-06-13 2021-12-21 Q-莱纳公司 用于检测和表征微生物的方法
GB201507026D0 (en) 2015-04-24 2015-06-10 Linea Ab Q Medical sample transportation container
GB201511129D0 (en) 2015-06-24 2015-08-05 Linea Ab Q Method of determining antimicrobial susceptibility of a microorganism
GB2554767A (en) 2016-04-21 2018-04-11 Q Linea Ab Detecting and characterising a microorganism
RU2659197C2 (ru) * 2016-07-18 2018-06-28 ФГБУ "Новосибирский научно-исследовательский институт туберкулеза" Министерства здравоохранения Российской Федерации Способ выделения ДНК клинических изолятов Mycobacterium tuberculosis из ткани легкого
GB201617713D0 (en) 2016-10-19 2016-11-30 Q-Linea Ab Method for recovering microbial cells
GB201716883D0 (en) 2017-10-13 2017-11-29 Q-Linea Ab Method for determining the concentration of intact microorganisms in a sample
CN109439658A (zh) * 2018-12-27 2019-03-08 中国农业科学院北京畜牧兽医研究所 一种裂解液及其在液态奶中提取核酸的方法
US20230183777A1 (en) * 2021-12-13 2023-06-15 BioMed Technology Holdings Limited Hypoosmotic stabilizer for genomic dna of gut microbiota, and preparation method and use thereof
CN114657125B (zh) * 2022-04-29 2023-08-08 中国科学院海洋研究所 鲨鱼单个核细胞的分离方法、鲨鱼稀释液及其用途
EP4389911A1 (de) 2022-12-21 2024-06-26 Mobidiag Oy Verfahren und systeme zur isolierung eines analyten

Family Cites Families (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3745090A (en) * 1970-08-04 1973-07-10 Nasa Method of detecting and counting bacteria in body fluids
US5346999A (en) 1985-01-18 1994-09-13 Applied Biosystems, Inc. Method of nucleic acid extraction
US4673733A (en) * 1985-04-11 1987-06-16 Sudhish Chandra Treatment of biological and pharmaceutical products adsorbed on a solid phase with virus and pyrogen inactivating agents
US5234809A (en) * 1989-03-23 1993-08-10 Akzo N.V. Process for isolating nucleic acid
US5420017A (en) * 1991-01-24 1995-05-30 Orion Corporation Ltd. Method and kit for the detection of microorganisms
WO1992021747A1 (en) * 1991-06-03 1992-12-10 Arch Development Corporation Methods and compositions of genetic stress response systems
US5459253A (en) 1991-07-19 1995-10-17 Pharmacia P-L Biochemicals Inc. M-RNA purification
EP0587951B1 (de) * 1992-09-18 1997-12-17 AMERSHAM INTERNATIONAL plc Verfahren zum Auffangen von Zellkernen und dafür verwendete Vorrichtung
FR2698879B1 (fr) * 1992-12-09 1995-01-13 Rhone Poulenc Biochimie gellules modifiées au niveau du catabolisme de la bétaïne, préparation et utilisations, notamment pour la production de métabolites ou d'enzymes.
US20040132082A1 (en) * 1995-02-14 2004-07-08 Bio101 Method for isolating DNA
DE19607202A1 (de) * 1996-02-26 1997-08-28 Uwe Dr Michel Reagenzienkit zur Präparation von Nukleinsäuren
GB9709728D0 (en) * 1997-05-13 1997-07-02 Dynal As Single step method
US20010007153A1 (en) * 1997-06-16 2001-07-05 Jennifer June Brown Solid chimeric organs, animal models having same, process for preparing same, non-tumorigenic immortalized human cell lines, susceptible cells and cytopathic mammalian viruses
EP1069131B1 (de) * 1999-07-15 2006-03-15 Qiagen GmbH Verfahren zur Trennung von teilchenförmigen Substraten aus einer Lösung indem man den Teilchenverlust minimiert
WO2001044298A1 (en) * 1999-12-13 2001-06-21 Zlb Bioplasma Ag Improvement of therapeutic potential of immunoglobulin preparations
GB0001450D0 (en) * 2000-01-21 2000-03-08 Genpoint As Cell isolation method
US7322369B2 (en) * 2000-04-11 2008-01-29 The United States Of America As Represented By The Secretary Of Agriculture Methods of detaching microorganisms from, or of inhibiting microbial attachment to, animal or poultry carcasses or seafood or parts thereof
AU2001259869A1 (en) 2000-05-18 2001-11-26 The Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services Centers For Disease Control And Prevention Method for the isolation of helicobacter pylori
EP1311860A2 (de) * 2000-08-21 2003-05-21 Queen's University At Kingston Verfahren und testkit zur auftrennung und zum nachweis von proteinen in biologischen proben
US7011943B2 (en) 2000-09-06 2006-03-14 Transnetyx, Inc. Method for detecting a designated genetic sequence in murine genomic DNA
AU2002230953A1 (en) * 2000-12-12 2002-06-24 Diversa Corporation Recombinant phytases and uses thereof
GB0030669D0 (en) * 2000-12-15 2001-01-31 Unilever Plc Detergent compositions
AU2002232858B2 (en) * 2000-12-22 2007-01-11 Sab, Llc Methods for cloning mammals using reprogrammed donor chromatin or donor cells
WO2002065125A1 (en) 2001-02-13 2002-08-22 Invitrogen Corporation Methods and compositions for isolation of biological macromolecules
JP3578144B2 (ja) * 2002-01-16 2004-10-20 住友電気工業株式会社 回折型光学部品の光学特性測定装置及び測定方法
AU2002249514B8 (en) * 2002-03-26 2008-10-09 Council Of Scientific And Industrial Research Primers for detecting food poisoning bacteria and a use thereof
US20040248089A1 (en) * 2002-03-27 2004-12-09 Banada Padmanabha Padmapriya Primes for detecting food poisoning bacteria and a method thereof
US20050009036A1 (en) * 2003-07-11 2005-01-13 Applera Corporation Methods and kits for obtaining nucleic acid from biological samples
WO2005010204A2 (en) * 2003-07-21 2005-02-03 Creighton University Rapid nucleic acid isolation method and compositions therefore
AU2003247060A1 (en) 2003-07-29 2005-02-14 All India Institute Of Medical Sciences (A.I.I.M.S.) A method for diagnosis of tuberculosis by smear microscopy, culture and polymerase chain reaction using processed clinical samples and kit thereof
US7547526B2 (en) * 2004-03-15 2009-06-16 Purdue Research Foundation Cell concentration and pathogen recovery
WO2006026248A1 (en) * 2004-08-25 2006-03-09 Sigma-Aldrich Co. Compositions and methods employing zwitterionic detergent combinations
DE102005040347A1 (de) * 2005-08-25 2007-03-01 Profos Ag Verfahren und Mittel zur Anreicherung, Entfernung und zum Nachweis von Listerien
US20080131955A1 (en) * 2006-11-30 2008-06-05 Canon U.S. Life Sciences, Inc. Method of Separating Target DNA from Mixed DNA

Also Published As

Publication number Publication date
US9328326B2 (en) 2016-05-03
US20100184210A1 (en) 2010-07-22
EP2049677B1 (de) 2011-07-20
EP2049677A1 (de) 2009-04-22
WO2008017097A1 (en) 2008-02-14

Similar Documents

Publication Publication Date Title
ATE517192T1 (de) Verfahren zur isolierung von zellen
WO2009123762A3 (en) Method of screening single cells for the production of biologically active agents
EP4249605A3 (de) Verfahren zum nachweis von analyten
WO2012155072A3 (en) Isolation of nucleic acids
WO2010132453A3 (en) Methods and compositions for analyte detection
WO2011021102A3 (en) Compositions and methods for intramolecular nucleic acid rearrangement
DE602007005496D1 (de) Ezymatisches verfahren zur herstellung von 2-o-glyceryl-alpha-d-glucopyranosid
WO2010039802A3 (en) Methods and compositions for isolating nucleic acid
WO2007106545A3 (en) Propagation of primary cells
WO2013096838A3 (en) Systems and methods for isolating nucleic acids
GB2485325A (en) Sample plate
MX2010008099A (es) Anticuerpos y angiopoyetina-2 estabilizados y sus usos.
WO2007098184A3 (en) Methods and compositions for analyte detection
WO2007130646A3 (en) Hcv coreceptor and methods of use thereof
WO2010048190A3 (en) Antibodies that bind to il-12 and methods of purifying the same
HK1256342A1 (zh) 环境或生物样本中的核酸纯化
DK2089516T3 (da) Fremgangsmåder til at forbedre indføringen af DNA i bakterielle celler
ATE500503T1 (de) Cd86- und cd80-rezeptorkonkurrenztests
WO2008085962A3 (en) Cell culture methods for producing recombinant proteins in the presence of reduced levels of one or more contaminants
WO2013004710A3 (en) Reagent usable for the isolation and/or purification of nucleic acids
WO2015035239A3 (en) Reagents and methods for screening mps i, ii, iiia, iiib, iva, vi, and vii
WO2013090386A3 (en) Methods and kits for room temperature in situ detection of a target nucleic acid in a biological sample
WO2010026169A3 (de) Verfahren zum isolieren und reinigen von nukleinsäuren
WO2012034130A3 (en) Methods and compositions for nucleic acid detection
WO2008008858A3 (en) Cellspot™ applications

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification

Ref document number: 2049677

Country of ref document: EP