CN102498781B - Method for Monochasma sheareri Maxim.ex Franch.et Savat seed germination and seedling breeding - Google Patents

Method for Monochasma sheareri Maxim.ex Franch.et Savat seed germination and seedling breeding Download PDF

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Publication number
CN102498781B
CN102498781B CN201110305020.6A CN201110305020A CN102498781B CN 102498781 B CN102498781 B CN 102498781B CN 201110305020 A CN201110305020 A CN 201110305020A CN 102498781 B CN102498781 B CN 102498781B
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seed
germination
savatier monochasma
monochasma herb
seeds
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CN102498781A (en
Inventor
张慕菡
马国华
张新华
黄辉球
黄韦耀
黄志刚
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Jiuhui Pharmaceutical Co ltd
South China Botanical Garden of CAS
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Jiuhui Pharmaceutical Co ltd
South China Botanical Garden of CAS
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Abstract

The invention discloses a method for Monochasma sheareri Maxim.ex Franch.et Savat seed germination and seedling breeding. The method comprises the following steps: soaking Monochasma sheareri Maxim.ex Franch.et Savat seeds in 50-3000 muM gibberellin for 24-48 hours, wherein the soaking process is performed in natural lighting; broadcast-sowing the soaked seeds on a surface of sand-containing red earth, covering a layer of a shading net above the red earth, watering or spraying for moisture retention; the germination temperature of the seeds is 20-35 DEG C. The Monochasma sheareri Maxim.ex Franch.et Savat seeds of the invention have a certain dormant period; by the combination of natural lighting (lighting is necessary for the gibberellin treatment and germination process, or germination is absolutely impossible) with gibberellin treatment, the invention completely breaks the dormancy; the germination rate for fresh seeds is up to above 90%; 2-4 months are taken for the seeds to grow into plants with a height of 10-20 cm from seed germination; no plant transplantation is needed; the survival rate is high; the method has the advantages of simplicity, convenience, rapidness and effectiveness; a lot of seedlings can be bred within a very short period, and raw materials required for medicine can be obtained.

Description

A kind of savatier monochasma herb seed germination and the method for growing seedlings
Technical field:
The invention belongs to plant propagation technology, a kind of method that is specifically related to savatier monochasma herb seed germination and grows seedlings.
Background technology:
Savatier monochasma herb (Monochasma sheareri Maxim.ex Franch.et Savat) belongs to herbaceos perennial for Scrophulariaceae savatier monochasma herb.Bitter, cool in nature.For cough, spit blood, dysentery characterized by blood in the stool, under band, gingivitis, mastitis, the treatment of the diseases such as furuncle.It is mainly distributed in the ground such as Shandong, Jiangsu, Anhui, Zhejiang, Jiangxi, Hubei.The demand that is difficult to meet domestic pharmaceutical production by excavating wild resource, also makes the natural distribution of savatier monochasma herb and growth supply fewer and feweri.Therefore be badly in need of seeking a kind of tame mating system.At present domestic patent and the research report of carrying out savatier monochasma herb sapling multiplication technology that there is no.
Summary of the invention:
The object of this invention is to provide and a kind ofly break savatier monochasma herb dormancy and make its sprouting, and the savatier monochasma herb seed germination that germination rate is high and the method for growing seedlings, that the method has is simple and convenient, advantage fast and effectively, can within very short time, breed large quantities of seed seedlings and obtain the required raw material of medicine, therefore be beneficial to popularization.
The present invention is understanding on the basis of savatier monochasma herb seed physiology characteristic, by utilizing plant growth regulator and natural lighting, savatier monochasma herb seed is processed, break the dormancy of savatier monochasma herb seed, it is sprouted, and germination rate is very high, in milpa appropriateness, continue spraying and moisturizing management again, seedling, after 2~3 months, can grow into 10~20cm high, substantially reaches the needs of commercially producing, realize the artificial cultivation of savatier monochasma herb, thereby reached object of the present invention.
Savatier monochasma herb seed germination of the present invention and the method for growing seedlings, is characterized in that, comprises the following steps:
(1) savatier monochasma herb seed is dipped in the gibberellin of 50~3000 μ M to 24~48 hours, immersion process is under natural lighting;
(2) seed broadcasting after soaking is arrived containing sandy Red Soil Surface, above red soil, cover one deck shading net, water or spraying and moisturizing;
The Germination Temperature of seed is 20~35 ℃.
Savatier monochasma herb seed is through the immersion treatment of gibberellin, broadcast sowing in containing sandy Red Soil Surface, within 3 days, just start afterwards fragmentary sprouting, after general two weeks, just can observe and emerge, then continue that appropriateness is watered or spraying and moisturizing management, seedling, after 2~3 months, can grow into 10~20cm high, substantially reaches the needs of commercially producing.After plant strain growth tends towards stability, can suitably increase illumination, partly or entirely open shading net, be beneficial to plant and receive more sunlight.
The concentration of described gibberellin is preferably 400~800 μ M, and the savatier monochasma herb seed germination rate of processing through this concentration is the highest.
It is 90% shading net that described shading net is preferably shading rate, is placed on 2 meters of, Red Soil Surface top.
In savatier monochasma herb seed germination process of growth, application of organic fertilizers or chemical fertilizer, fertilizer or chemical fertilizer are conducive to the growth of plant.
Described immersion later savatier monochasma herb seed with the suspension of seed or mix upper fine sand and broadcast sowing and contain sandy Red Soil Surface.
Described savatier monochasma herb select seeds is fresh savatier monochasma herb seed, and fresh savatier monochasma herb seed is after processing of the present invention, and germination rate can reach more than 90%.If the words of the savatier monochasma herb seed of storage, preferably be placed in 10~-20 ℃ of low-temperature preservations, the savatier monochasma herb seed of-20 ℃ of low-temperature preservations was placed after 2 years, after the present invention processes, germination rate can also reach 50%, and savatier monochasma herb seed is preserved 3 months at normal temperature (20~35 ℃), germination rate only has 10%; 10 ℃ of storages 3 months, the highest germination rate only had 52%, so low temperature storage can extend seed vitality, maintains higher germination rate.
Seed is the brood body of plant, and it has certain resting stage and vitality conventionally, and how breaking dormancy, takes different means according to the character of different seeds.There is certain resting stage in savatier monochasma herb seed of the present invention, the present invention is by (having illumination in gibberellin processing and germination process in conjunction with natural lighting, otherwise can not sprout completely) and gibberellin processing, can break its dormancy completely, the germination rate of fresh seeds can reach more than 90%, from seed germination, to growing to the plant that 10~20cm is high, only need 2~3 months, and plant is without transplanting, survival rate is high, there is simple and convenient, advantage fast and effectively, can within very short time, breed large quantities of seed seedlings and obtain the required raw material of medicine.
Embodiment:
Following examples are to further illustrate of the present invention, rather than limitation of the present invention.
Embodiment 1:
South China Botanical Garden Chinese Academy of Sciences savatier monochasma herb plantation base, Guangzhou.
The natural temperature that seed soaks and sprouts is between 20~35 ℃.
Get fresh savatier monochasma herb seed, the gibberellin aqueous solution with 600 μ M, under Indoor Natural illumination condition, after immersion treatment 24 hours, with the suspension of this seed, water and be sprinkling upon containing sandy Red Soil Surface, above red soil top layer, to cover one deck shading rate be 90% shading net two meters of, then water every day or spraying and moisturizing, two weeks seed germination rate reach 90%, 2-3 after the month seed germination to growing into the plant strain growth that 10-20 centimetre is high, substantially reach the raw material needs that commercialization is produced.In plantation germination process, suitably manure use or chemical fertilizer, be conducive to the growth of plant.After plant strain growth tends towards stability, can suitably increase illumination, partially open shading net, be beneficial to plant and receive more sunlight.
Embodiment 2:
South China Botanical Garden Chinese Academy of Sciences savatier monochasma herb plantation base, Guangzhou.
The natural temperature that seed soaks and sprouts is between 20~35 ℃.
Get fresh savatier monochasma herb seed, the gibberellin aqueous solution with 50 μ M, under natural lighting, after immersion treatment 24 hours, with the suspension of this seed, water and be sprinkling upon containing sandy Red Soil Surface, above red soil top layer, to cover one deck shading rate be 90% shading net two meters of, then water every day or spraying and moisturizing, after two weeks seed germination rate reach 78%, 2-3 after the month seed germination to growing into the plant strain growth that 10-20 centimetre is high, substantially reach the raw material needs that commercialization is produced.
Embodiment 3:
South China Botanical Garden Chinese Academy of Sciences savatier monochasma herb plantation base, Guangzhou.
The natural temperature that seed soaks and sprouts is between 20~35 ℃.
Get fresh savatier monochasma herb seed, the gibberellin aqueous solution with 3000 μ M, under natural lighting, after immersion treatment 24 hours, with the suspension of this seed, water and be sprinkling upon containing sandy Red Soil Surface, above red soil top layer, to cover one deck shading rate be 90% shading net two meters of, then water every day or spraying and moisturizing, two weeks seed germination rate reach 64%, 2-3 after the month seed germination to growing into the plant strain growth that 10-20 centimetre is high, substantially reach the raw material needs that commercialization is produced.
Embodiment 4:
South China Botanical Garden Chinese Academy of Sciences savatier monochasma herb plantation base, Guangzhou.
The natural temperature that seed soaks and sprouts is between 20~35 ℃.
Get fresh savatier monochasma herb seed, the gibberellin aqueous solution with 400 μ M, under natural lighting, after immersion treatment 24 hours, with the suspension of this seed, water and be sprinkling upon containing sandy Red Soil Surface, above red soil top layer, to cover one deck shading rate be 90% shading net two meters of, then water every day or spraying and moisturizing, two weeks seed germination rate reach 92%, 2-3 after the month seed germination to growing into the plant strain growth that 10-20 centimetre is high, substantially reach the raw material needs that commercialization is produced.
Embodiment 5:
Savatier monochasma herb planting base, Xingguo County, Ganzhou City, Jiangxi Province.
The natural temperature that seed soaks and sprouts is between 20~35 ℃.
By fresh savatier monochasma herb seed through 10 ℃ of low temperature treatment, after 3 months, take out, the gibberellin aqueous solution with 800 μ M, under natural lighting, after immersion treatment 48 hours, with the upper fine sand of this seed companion, water and be sprinkling upon containing sandy Red Soil Surface, above red soil top layer, to cover one deck shading rate be 90% shading net two meters of, then water every day or spraying and moisturizing, two weeks seed germination rate reaches 52%, 2-3 after the month seed germination to growing into the plant strain growth that 10-20 centimetre is high, substantially reach the raw material needs that commercialization is produced.
Embodiment 6:
Savatier monochasma herb planting base, Xingguo County, Ganzhou City, Jiangxi Province.
The natural temperature that seed soaks and sprouts is between 20~35 ℃.
By-20 ℃ of low temperature treatment of fresh savatier monochasma herb seed warp, within 2 years, take out afterwards, the gibberellin aqueous solution with 700 μ M, under natural lighting, after immersion treatment 24 hours, with the upper fine sand of this seed companion, water and be sprinkling upon the outdoor sandy Red Soil Surface that contains, above red soil top layer, to cover one deck shading rate be 90% shading net two meters of, then water every day or spraying and moisturizing, two weeks seed germination rate reaches 50%, 2-3 after the month seed germination to growing into the plant strain growth that 10-20 centimetre is high, substantially reach the raw material needs that commercialization is produced.

Claims (5)

1. savatier monochasma herb seed germination and a method of growing seedlings, is characterized in that, comprises the following steps:
(1) savatier monochasma herb (Monochasma sheareri Maxim.ex Franch.et Savat) seed is dipped in the gibberellin of 50~3000 μ M to 24~48 hours, immersion process is under natural lighting;
(2) seed broadcasting after soaking is arrived containing sandy Red Soil Surface, above red soil, cover one deck shading net, water or spraying and moisturizing;
The Germination Temperature of seed is 20~35 ℃.
2. savatier monochasma herb seed germination according to claim 1 and the method for growing seedlings, is characterized in that, described gibberellin concentration is 400~800 μ M.
3. savatier monochasma herb seed germination according to claim 1 and the method for growing seedlings, is characterized in that, described shading net is the shading net of shading rate 90%, is placed on 2 meters of, Red Soil Surface top.
4. savatier monochasma herb seed germination according to claim 1 and the method for growing seedlings, is characterized in that, described by the seed broadcasting after soaking to being with the suspension of seed containing sandy Red Soil Surface or mixing upper fine sand and broadcast sowing and contain sandy Red Soil Surface.
5. savatier monochasma herb seed germination according to claim 1 and the method for growing seedlings, is characterized in that, described savatier monochasma herb seed is fresh savatier monochasma herb seed or the savatier monochasma herb seed that is placed in 10~-20 ℃ of low-temperature preservations.
CN201110305020.6A 2011-10-09 2011-10-09 Method for Monochasma sheareri Maxim.ex Franch.et Savat seed germination and seedling breeding Active CN102498781B (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111386981A (en) * 2020-04-10 2020-07-10 惠州市九惠制药股份有限公司 Method for planting savatier saururus in pot

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* Cited by examiner, † Cited by third party
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CN110495391A (en) * 2019-07-26 2019-11-26 中国林业科学研究院亚热带林业实验中心 A kind of sterile high efficiency seedling cultivating method of savatier monochasma herb
CN111226704B (en) * 2020-03-12 2022-03-18 惠州市九惠制药股份有限公司 Cutting seedling raising method for savatier shawense
CN111727823B (en) * 2020-07-09 2022-01-25 江西神元湖农业科技发展有限公司 Large-scale seedling cultivation method for savatier sauropus

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US20040063582A1 (en) * 2002-09-17 2004-04-01 Johnson William S. Seed treatment and method of treating seed

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CN100594773C (en) * 2006-05-19 2010-03-24 四川阿坝天贝生物有限责任公司 Method for processing sown tendril-leaved fritillary bulb
CN101507415B (en) * 2009-03-26 2011-01-05 浙江大学 In-vitro culture method of antlerpilose grass

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Publication number Priority date Publication date Assignee Title
US20040063582A1 (en) * 2002-09-17 2004-04-01 Johnson William S. Seed treatment and method of treating seed

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111386981A (en) * 2020-04-10 2020-07-10 惠州市九惠制药股份有限公司 Method for planting savatier saururus in pot

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