CN102443629A - 一种沙门氏菌荧光定量pcr检测试剂盒及其检测方法 - Google Patents
一种沙门氏菌荧光定量pcr检测试剂盒及其检测方法 Download PDFInfo
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- CN102443629A CN102443629A CN2011103413750A CN201110341375A CN102443629A CN 102443629 A CN102443629 A CN 102443629A CN 2011103413750 A CN2011103413750 A CN 2011103413750A CN 201110341375 A CN201110341375 A CN 201110341375A CN 102443629 A CN102443629 A CN 102443629A
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Abstract
Description
10×PCR Buffer | 2.5μL |
2.5mM dNTP | 2μL |
0.03 mM上游引物 | 0.5μL |
0.03 mM 下游引物 | 0.5μL |
0.015 mM荧光探针 | 0.3μL |
5U/μL Taq酶 | 0.2μL |
DNA模板 | 2μL |
ddH2O | 18.8μL |
总反应体系 | 25μL |
样本编号 | 传统培养法 | 荧光定量PCR法 | 样本编号 | 传统培养法 | 荧光定量PCR法 |
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Claims (5)
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Cited By (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103114148A (zh) * | 2013-03-05 | 2013-05-22 | 扬州大学 | 一种沙门菌荧光定量pcr检测和分型的引物、探针及试剂盒 |
CN103233070A (zh) * | 2013-04-25 | 2013-08-07 | 广州弗赛生物科技有限公司 | 用于沙门氏菌实时定量pcr检测的特异性引物、探针及试剂盒 |
CN103243159A (zh) * | 2013-04-11 | 2013-08-14 | 深圳职业技术学院 | 具毒性质粒沙门氏菌荧光pcr检测探针、试剂盒及检测方法 |
CN103320507A (zh) * | 2013-07-01 | 2013-09-25 | 黑龙江出入境检验检疫局检验检疫技术中心 | 利用dpo-pcr方法检测沙门氏菌的dpo引物序列及其检测试剂盒 |
CN103484548A (zh) * | 2013-09-15 | 2014-01-01 | 山东博奥克生物科技有限公司 | 沙门氏菌荧光定量pcr检测试剂盒及其检测方法 |
CN106755494A (zh) * | 2017-01-26 | 2017-05-31 | 福建出入境检验检疫局检验检疫技术中心 | 蜂房蜜蜂球菌荧光定量pcr检测试剂盒及其检测方法 |
CN107699629A (zh) * | 2017-11-23 | 2018-02-16 | 深圳市草履虫生物科技有限公司 | 检测空肠弯曲菌、沙门氏菌和单增李斯特菌的复合荧光pcr检测用引物及探针、试剂盒 |
CN108424976A (zh) * | 2018-02-08 | 2018-08-21 | 杭州富集生物科技有限公司 | 突发急性烈性传染病应急检测储备试剂盒及检验方法 |
CN109652575A (zh) * | 2019-03-05 | 2019-04-19 | 许绍坤 | 一种针对沙门氏菌的核酸快速检测方法 |
CN110229920A (zh) * | 2019-07-07 | 2019-09-13 | 福建省产品质量检验研究院 | 一种饲料中两歧双歧杆菌的鉴定方法 |
CN112538543A (zh) * | 2020-12-16 | 2021-03-23 | 山东农业大学 | 用于检测沙门氏菌属的特异性引物及其试剂盒 |
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CN101440391A (zh) * | 2008-07-04 | 2009-05-27 | 中华人民共和国汕头出入境检验检疫局 | 用于沙门菌、副溶血弧菌和大肠杆菌o157:h7多重荧光pcr同步检测的引物和探针序列 |
CN101705281A (zh) * | 2009-11-19 | 2010-05-12 | 浙江省疾病预防控制中心 | 检测沙门氏菌的实时荧光pcr试剂盒及检测方法 |
CN102154497A (zh) * | 2011-03-18 | 2011-08-17 | 麻丽丹 | 霍乱弧菌、副溶血性弧菌和沙门氏菌的m-PCR引物和探针及检测方法 |
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Patent Citations (3)
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CN101440391A (zh) * | 2008-07-04 | 2009-05-27 | 中华人民共和国汕头出入境检验检疫局 | 用于沙门菌、副溶血弧菌和大肠杆菌o157:h7多重荧光pcr同步检测的引物和探针序列 |
CN101705281A (zh) * | 2009-11-19 | 2010-05-12 | 浙江省疾病预防控制中心 | 检测沙门氏菌的实时荧光pcr试剂盒及检测方法 |
CN102154497A (zh) * | 2011-03-18 | 2011-08-17 | 麻丽丹 | 霍乱弧菌、副溶血性弧菌和沙门氏菌的m-PCR引物和探针及检测方法 |
Cited By (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103114148B (zh) * | 2013-03-05 | 2014-09-24 | 扬州大学 | 一种沙门菌荧光定量pcr检测和分型的引物、探针及试剂盒 |
CN103114148A (zh) * | 2013-03-05 | 2013-05-22 | 扬州大学 | 一种沙门菌荧光定量pcr检测和分型的引物、探针及试剂盒 |
CN103243159A (zh) * | 2013-04-11 | 2013-08-14 | 深圳职业技术学院 | 具毒性质粒沙门氏菌荧光pcr检测探针、试剂盒及检测方法 |
CN103233070A (zh) * | 2013-04-25 | 2013-08-07 | 广州弗赛生物科技有限公司 | 用于沙门氏菌实时定量pcr检测的特异性引物、探针及试剂盒 |
CN103320507B (zh) * | 2013-07-01 | 2015-01-07 | 黑龙江出入境检验检疫局检验检疫技术中心 | 利用dpo-pcr方法检测沙门氏菌的dpo引物序列及其检测试剂盒 |
CN103320507A (zh) * | 2013-07-01 | 2013-09-25 | 黑龙江出入境检验检疫局检验检疫技术中心 | 利用dpo-pcr方法检测沙门氏菌的dpo引物序列及其检测试剂盒 |
CN103484548A (zh) * | 2013-09-15 | 2014-01-01 | 山东博奥克生物科技有限公司 | 沙门氏菌荧光定量pcr检测试剂盒及其检测方法 |
CN106755494A (zh) * | 2017-01-26 | 2017-05-31 | 福建出入境检验检疫局检验检疫技术中心 | 蜂房蜜蜂球菌荧光定量pcr检测试剂盒及其检测方法 |
CN107699629A (zh) * | 2017-11-23 | 2018-02-16 | 深圳市草履虫生物科技有限公司 | 检测空肠弯曲菌、沙门氏菌和单增李斯特菌的复合荧光pcr检测用引物及探针、试剂盒 |
CN108424976A (zh) * | 2018-02-08 | 2018-08-21 | 杭州富集生物科技有限公司 | 突发急性烈性传染病应急检测储备试剂盒及检验方法 |
CN109652575A (zh) * | 2019-03-05 | 2019-04-19 | 许绍坤 | 一种针对沙门氏菌的核酸快速检测方法 |
CN110229920A (zh) * | 2019-07-07 | 2019-09-13 | 福建省产品质量检验研究院 | 一种饲料中两歧双歧杆菌的鉴定方法 |
CN112538543A (zh) * | 2020-12-16 | 2021-03-23 | 山东农业大学 | 用于检测沙门氏菌属的特异性引物及其试剂盒 |
CN112538543B (zh) * | 2020-12-16 | 2022-09-27 | 山东农业大学 | 用于检测沙门氏菌属的特异性引物及其试剂盒 |
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