CN101356288B - 使用孔的方法 - Google Patents

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Publication number
CN101356288B
CN101356288B CN200680050878.XA CN200680050878A CN101356288B CN 101356288 B CN101356288 B CN 101356288B CN 200680050878 A CN200680050878 A CN 200680050878A CN 101356288 B CN101356288 B CN 101356288B
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nucleotide
misc
feature
monophosphic
sequence
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CN101356288A (zh
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H·贝利
Y·阿斯泰尔
O·布雷厄
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Oxford Innovation Co Ltd, University of
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Oxford University Innovation Ltd
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483Physical analysis of biological material
    • G01N33/487Physical analysis of biological material of liquid biological material
    • G01N33/48707Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48721Investigating individual macromolecules, e.g. by translocation through nanopores
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483Physical analysis of biological material
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60Detection means characterised by use of a special device
    • C12Q2565/631Detection means characterised by use of a special device being a biochannel or pore

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biotechnology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Nanotechnology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
CN200680050878.XA 2005-11-15 2006-11-15 使用孔的方法 Active CN101356288B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB0523282.2 2005-11-15
GBGB0523282.2A GB0523282D0 (en) 2005-11-15 2005-11-15 Methods using pores
PCT/GB2006/004265 WO2007057668A1 (en) 2005-11-15 2006-11-15 Methods using pores

Publications (2)

Publication Number Publication Date
CN101356288A CN101356288A (zh) 2009-01-28
CN101356288B true CN101356288B (zh) 2016-02-03

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US (2) US8105846B2 (enExample)
EP (2) EP2272981B1 (enExample)
JP (1) JP5328362B2 (enExample)
KR (1) KR20080083281A (enExample)
CN (1) CN101356288B (enExample)
AT (1) ATE545712T1 (enExample)
AU (1) AU2006314296B2 (enExample)
BR (1) BRPI0618391A2 (enExample)
CA (1) CA2629009C (enExample)
GB (1) GB0523282D0 (enExample)
IL (1) IL191319A (enExample)
MY (1) MY149161A (enExample)
NZ (1) NZ568163A (enExample)
RU (1) RU2008123842A (enExample)
UA (1) UA95087C2 (enExample)
WO (1) WO2007057668A1 (enExample)

Families Citing this family (90)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0523282D0 (en) 2005-11-15 2005-12-21 Isis Innovation Methods using pores
US9121843B2 (en) 2007-05-08 2015-09-01 Trustees Of Boston University Chemical functionalization of solid-state nanopores and nanopore arrays and applications thereof
GB2453377A (en) * 2007-10-05 2009-04-08 Isis Innovation Transmembrane protein pores and molecular adapters therefore.
EP2307540B1 (en) * 2008-07-07 2017-04-19 Oxford Nanopore Technologies Limited Enzyme-pore constructs
WO2010004273A1 (en) * 2008-07-07 2010-01-14 Oxford Nanopore Technologies Limited Base-detecting pore
EP2851433B1 (en) * 2008-08-01 2017-10-11 Brown University System and methods for determining molecules using mass spectrometry and related techniques
GB0820927D0 (en) * 2008-11-14 2008-12-24 Isis Innovation Method
WO2010062903A2 (en) * 2008-11-26 2010-06-03 Board Of Regents, The University Of Texas System Genomic sequencing using modified protein pores and ionic liquids
KR20110125226A (ko) 2009-01-30 2011-11-18 옥스포드 나노포어 테크놀로지즈 리미티드 혼성화 링커
JP5843614B2 (ja) * 2009-01-30 2016-01-13 オックスフォード ナノポア テクノロジーズ リミテッド 膜貫通配列決定における核酸構築物のためのアダプター
GB0905140D0 (en) * 2009-03-25 2009-05-06 Isis Innovation Method
US8986928B2 (en) 2009-04-10 2015-03-24 Pacific Biosciences Of California, Inc. Nanopore sequencing devices and methods
US9017937B1 (en) 2009-04-10 2015-04-28 Pacific Biosciences Of California, Inc. Nanopore sequencing using ratiometric impedance
CA2808576A1 (en) 2009-09-30 2011-04-07 Quantapore, Inc. Ultrafast sequencing of biological polymers using a labeled nanopore
CN102741430B (zh) 2009-12-01 2016-07-13 牛津楠路珀尔科技有限公司 生化分析仪器、用于进行生化分析的第一模块以及相关方法
GB2490846A (en) * 2010-03-05 2012-11-14 Univ Utah Res Found Detection of nucleic acid lesions and adducts using nanopores
US9005425B2 (en) 2010-03-05 2015-04-14 University Of Utah Research Foundation Detection of nucleic acid lesions and adducts using nanopores
US8652779B2 (en) 2010-04-09 2014-02-18 Pacific Biosciences Of California, Inc. Nanopore sequencing using charge blockade labels
CN103370617B (zh) 2010-10-01 2015-11-25 牛津纳米孔技术有限公司 生物化学分析设备和旋转阀
US8673757B2 (en) 2010-10-28 2014-03-18 International Business Machines Corporation Structure and method for using high-k material as an etch stop layer in dual stress layer process
CN103460040B (zh) 2011-02-11 2016-08-17 牛津纳米孔技术有限公司 突变型孔
WO2012164270A1 (en) 2011-05-27 2012-12-06 Oxford Nanopore Technologies Limited Coupling method
RU2467218C1 (ru) * 2011-06-16 2012-11-20 Закрытое Акционерное Общество "Научно-производственное объединение "Гидравлические аппараты" (ЗАО "НПО "Гидроаппарат") Гидромуфта
EP2988128B1 (en) 2011-07-20 2017-12-27 The Regents of the University of California Dual-pore device
IN2014DN00221A (enExample) 2011-07-25 2015-06-05 Oxford Nanopore Tech Ltd
EP2987870B1 (en) 2011-10-21 2020-02-19 Oxford Nanopore Technologies Limited Method of characterizing a target polynucleotide using a transmembrane pore and molecular motor
JP6228128B2 (ja) 2011-12-29 2017-11-08 オックスフォード ナノポール テクノロジーズ リミテッド 酵素法
CN104136631B (zh) 2011-12-29 2017-03-01 牛津纳米孔技术公司 使用xpd解旋酶表征多核苷酸的方法
IN2014DN06796A (enExample) 2012-02-15 2015-05-22 Oxford Nanopore Tech Ltd
SG11201405995YA (en) * 2012-04-02 2014-10-30 Lux Bio Group Inc Apparatus and method for molecular separation, purification, and sensing
KR102083695B1 (ko) 2012-04-10 2020-03-02 옥스포드 나노포어 테크놀로지즈 리미티드 돌연변이체 리세닌 기공
US10808231B2 (en) 2012-07-19 2020-10-20 Oxford Nanopore Technologies Limited Modified helicases
US9797009B2 (en) 2012-07-19 2017-10-24 Oxford Nanopore Technologies Limited Enzyme construct
WO2014013259A1 (en) * 2012-07-19 2014-01-23 Oxford Nanopore Technologies Limited Ssb method
US9551023B2 (en) 2012-09-14 2017-01-24 Oxford Nanopore Technologies Ltd. Sample preparation method
US9651539B2 (en) 2012-10-28 2017-05-16 Quantapore, Inc. Reducing background fluorescence in MEMS materials by low energy ion beam treatment
WO2014072703A1 (en) 2012-11-06 2014-05-15 Oxford Nanopore Technologies Limited Quadruplex method
GB201318465D0 (en) 2013-10-18 2013-12-04 Oxford Nanopore Tech Ltd Method
EP2964779B1 (en) 2013-03-08 2018-08-29 Oxford Nanopore Technologies Limited Use of spacer elements in a nucleic acid to control movement of a helicase
GB201314695D0 (en) 2013-08-16 2013-10-02 Oxford Nanopore Tech Ltd Method
GB201313477D0 (en) 2013-07-29 2013-09-11 Univ Leuven Kath Nanopore biosensors for detection of proteins and nucleic acids
CN103267785B (zh) * 2013-05-14 2016-04-13 西北大学 一种检测药物包裹或释放过程的单分子分析方法
EP3004378B1 (en) 2013-05-24 2017-12-20 Illumina Cambridge Limited Pyrophosphorolytic sequencing using nanopores
WO2014190322A2 (en) 2013-05-24 2014-11-27 Quantapore, Inc. Nanopore-based nucleic acid analysis with mixed fret detection
GB201316849D0 (en) 2013-09-23 2013-11-06 Isis Innovation Method
CN118086476A (zh) 2013-10-18 2024-05-28 牛津纳米孔科技公开有限公司 经修饰的酶
GB201406151D0 (en) 2014-04-04 2014-05-21 Oxford Nanopore Tech Ltd Method
GB201406155D0 (en) 2014-04-04 2014-05-21 Oxford Nanopore Tech Ltd Method
CN111534504B (zh) 2014-01-22 2024-06-21 牛津纳米孔科技公开有限公司 将一个或多个多核苷酸结合蛋白连接到靶多核苷酸的方法
GB201403096D0 (en) 2014-02-21 2014-04-09 Oxford Nanopore Tech Ltd Sample preparation method
CN106460061B (zh) 2014-04-04 2020-03-06 牛津纳米孔技术公司 在双链核酸分子的两端使用纳米孔和锚分子来表征所述双链核酸的方法
GB201417712D0 (en) 2014-10-07 2014-11-19 Oxford Nanopore Tech Ltd Method
CN106459159B (zh) 2014-05-02 2021-11-30 牛津纳米孔技术公司 突变孔
GB201411285D0 (en) 2014-06-25 2014-08-06 Prosser Joseph Sequencer
US10400014B2 (en) 2014-09-01 2019-09-03 Oxford Nanopore Technologies Ltd. Mutant CsgG pores
WO2016055778A1 (en) 2014-10-07 2016-04-14 Oxford Nanopore Technologies Limited Mutant pores
WO2016057829A1 (en) 2014-10-10 2016-04-14 Quantapore, Inc. Nanopore-based polymer analysis with mutually-quenching fluorescent labels
GB201418159D0 (en) 2014-10-14 2014-11-26 Oxford Nanopore Tech Ltd Method
GB201418469D0 (en) 2014-10-17 2014-12-03 Oxford Nanopore Tech Ltd Method
KR102457147B1 (ko) 2014-10-17 2022-10-19 옥스포드 나노포어 테크놀로지즈 피엘씨 나노 세공 rna 특징 규명을 위한 방법
AU2015335616B2 (en) 2014-10-24 2019-09-12 Quantapore, Inc. Efficient optical analysis of polymers using arrays of nanostructures
WO2016069806A2 (en) 2014-10-31 2016-05-06 Genia Technologies, Inc. Alpha-hemolysin variants with altered characteristics
US10060903B2 (en) * 2014-11-05 2018-08-28 Genia Technologies, Inc. Exporting measurements of nanopore arrays
US9557294B2 (en) 2014-12-19 2017-01-31 Genia Technologies, Inc. Nanopore-based sequencing with varying voltage stimulus
US9863904B2 (en) 2014-12-19 2018-01-09 Genia Technologies, Inc. Nanopore-based sequencing with varying voltage stimulus
EP3254109A4 (en) * 2015-02-02 2018-07-04 Two Pore Guys, Inc. Labile linkers for biomarker detection
GB201502809D0 (en) 2015-02-19 2015-04-08 Oxford Nanopore Tech Ltd Mutant pore
GB201502810D0 (en) 2015-02-19 2015-04-08 Oxford Nanopore Tech Ltd Method
CN107735686B (zh) 2015-04-14 2021-06-11 鲁汶天主教大学 具有内部蛋白质衔接子的纳米孔
US10126262B2 (en) 2015-09-24 2018-11-13 Genia Technologies, Inc. Differential output of analog memories storing nanopore measurement samples
JP6889701B2 (ja) 2015-09-24 2021-06-18 エフ.ホフマン−ラ ロシュ アーゲーF. Hoffmann−La Roche Aktiengesellschaft アルファ溶血素バリアント
CN105259229B (zh) * 2015-10-22 2018-04-20 清华大学 一种检测药物的单分子分析方法
US10280411B2 (en) 2015-10-27 2019-05-07 Pacific Biosciences of California, In.c Methods, systems, and reagents for direct RNA sequencing
CN109072295B (zh) 2015-12-08 2022-05-31 鲁汶大学研究与发展中心 修饰的纳米孔,包含其的组合物及其应用
JP6579948B2 (ja) * 2015-12-24 2019-09-25 株式会社日立ハイテクノロジーズ 生体ポリマを分析するための測定試薬及び分析デバイス
US10975428B2 (en) 2016-03-02 2021-04-13 Oxford Nanopore Technologies Ltd. Mutant pore
CN118326018B (zh) 2016-04-06 2025-11-07 牛津纳米孔科技公开有限公司 突变体孔
GB201609220D0 (en) 2016-05-25 2016-07-06 Oxford Nanopore Tech Ltd Method
EP4063521B1 (en) * 2016-05-25 2024-02-21 Oxford Nanopore Technologies PLC Method of nanopore sequencing
CN109477813A (zh) 2016-07-05 2019-03-15 昆塔波尔公司 基于光学的纳米孔测序
GB201619930D0 (en) 2016-11-24 2017-01-11 Oxford Nanopore Tech Apparatus and methods for controlling insertion of a membrane channel into a membrane
CN119530363A (zh) * 2017-02-10 2025-02-28 牛津纳米孔科技公开有限公司 修饰的纳米孔、包含其的组合物及其用途
EP4026916B1 (en) * 2017-04-19 2023-10-18 Electronic Biosciences Inc. Nanopore/nanowell electrode enabled exonuclease sequencing
GB201707122D0 (en) 2017-05-04 2017-06-21 Oxford Nanopore Tech Ltd Pore
CN117106037B (zh) 2017-06-30 2025-07-25 弗拉芒区生物技术研究所 新颖蛋白孔
JP7042910B2 (ja) 2017-11-27 2022-03-28 エフ.ホフマン-ラ ロシュ アーゲー ナノポア-sbs信号のための規格化およびベースラインシフト除去
GB201807793D0 (en) 2018-05-14 2018-06-27 Oxford Nanopore Tech Ltd Method
GB202202716D0 (en) 2022-02-28 2022-04-13 Oxford Nanopore Tech Plc Apparatus and methods for controlling insertion of a membrane channel into a membrane
AU2023320396A1 (en) 2022-08-02 2025-02-20 Glyphic Biotechnologies, Inc. Protein sequencing via coupling of polymerizable molecules
GB202401864D0 (en) 2024-02-12 2024-03-27 Fund Centre De Regulacio Genòmica A method of detecting non-canonical bases in sequencing data

Family Cites Families (41)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FI82266C (fi) 1982-10-19 1991-02-11 Cetus Corp Foerfarande foer framstaellning av il-2 -mutein.
EP0753071A1 (en) 1993-04-28 1997-01-15 Worcester Foundation For Experimental Biology Cell-targeted lytic pore-forming agents
US5777078A (en) 1993-04-28 1998-07-07 Worcester Foundation For Experimental Biology Triggered pore-forming agents
US5795782A (en) * 1995-03-17 1998-08-18 President & Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
US6362002B1 (en) 1995-03-17 2002-03-26 President And Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
WO1999005167A1 (en) * 1997-07-25 1999-02-04 University Of Massachusetts Designed protein pores as components for biosensors
US6087099A (en) 1997-09-08 2000-07-11 Myriad Genetics, Inc. Method for sequencing both strands of a double stranded DNA in a single sequencing reaction
US6127166A (en) 1997-11-03 2000-10-03 Bayley; Hagan Molluscan ligament polypeptides and genes encoding them
JPH11137260A (ja) 1997-11-06 1999-05-25 Soyaku Gijutsu Kenkyusho:Kk 抗インフルエンザウイルス環状ダンベル型rna−dnaキメラ化合物及び抗インフルエンザウイルス剤
US6123819A (en) 1997-11-12 2000-09-26 Protiveris, Inc. Nanoelectrode arrays
DE19826758C1 (de) 1998-06-15 1999-10-21 Soft Gene Gmbh Darstellung von linearen kovalent geschlossenen DNA-Molekülen als Expressionskonstrukte
US6267872B1 (en) * 1998-11-06 2001-07-31 The Regents Of The University Of California Miniature support for thin films containing single channels or nanopores and methods for using same
US6426231B1 (en) 1998-11-18 2002-07-30 The Texas A&M University System Analyte sensing mediated by adapter/carrier molecules
NO986133D0 (no) 1998-12-23 1998-12-23 Preben Lexow FremgangsmÕte for DNA-sekvensering
WO2000078668A1 (en) 1999-06-22 2000-12-28 President And Fellows Of Harvard College Control of solid state dimensional features
EP1196434A2 (en) 1999-06-29 2002-04-17 University Health Network Peptide conjugates for the stabilization of membrane proteins and interactions with biological membranes
CA2360342A1 (en) 1999-12-02 2001-06-07 Molecular Staging Inc. Generation of single-strand circular dna from linear self-annealing segments
EP2083015B1 (en) 2000-02-11 2016-04-06 The Texas A & M University System Biosensor compositions and methods of use
US7001792B2 (en) 2000-04-24 2006-02-21 Eagle Research & Development, Llc Ultra-fast nucleic acid sequencing device and a method for making and using the same
AU2002239284A1 (en) 2000-11-27 2002-06-03 The Regents Of The University Of California Methods and devices for characterizing duplex nucleic acid molecules
US7807408B2 (en) 2001-03-19 2010-10-05 President & Fellows Of Harvard College Directed evolution of proteins
DE60222404T2 (de) * 2001-05-29 2008-06-19 Asahi Glass Co., Ltd. Verfahren zur konstruktion eines wirts und verfahren zur herstellung eines fremdproteins
US6863833B1 (en) 2001-06-29 2005-03-08 The Board Of Trustees Of The Leland Stanford Junior University Microfabricated apertures for supporting bilayer lipid membranes
AU2003245272A1 (en) 2002-05-10 2003-11-11 The Texas A And M University System Stochastic sensing through covalent interactions
US7163658B2 (en) 2003-04-23 2007-01-16 Rouvain Bension Rapid sequencing of polymers
WO2005056750A2 (en) 2003-12-11 2005-06-23 Quark Biotech, Inc. Inversion-duplication of nucleic acids and libraries prepared thereby
WO2006028508A2 (en) 2004-03-23 2006-03-16 President And Fellows Of Harvard College Methods and apparatus for characterizing polynucleotides
CN101103357B (zh) 2004-08-13 2012-10-03 哈佛学院院长等 超高处理量光学-纳米孔dna读出平台
US7867716B2 (en) 2004-12-21 2011-01-11 The Texas A&M University System High temperature ion channels and pores
GB0505971D0 (en) 2005-03-23 2005-04-27 Isis Innovation Delivery of molecules to a lipid bilayer
GB0523282D0 (en) 2005-11-15 2005-12-21 Isis Innovation Methods using pores
ATE518010T1 (de) 2005-12-22 2011-08-15 Pacific Biosciences California Aktive oberflächengekoppelte polymerasen
US7932029B1 (en) 2006-01-04 2011-04-26 Si Lok Methods for nucleic acid mapping and identification of fine-structural-variations in nucleic acids and utilities
WO2008045575A2 (en) 2006-10-13 2008-04-17 J. Craig Venter Institute, Inc. Sequencing method
AU2008217578A1 (en) 2007-02-20 2008-08-28 Oxford Nanopore Technologies Limited Lipid bilayer sensor system
US20110005918A1 (en) 2007-04-04 2011-01-13 Akeson Mark A Compositions, devices, systems, and methods for using a nanopore
GB2453377A (en) 2007-10-05 2009-04-08 Isis Innovation Transmembrane protein pores and molecular adapters therefore.
EP3045542B1 (en) 2008-03-28 2016-11-16 Pacific Biosciences of California, Inc. Methods for nucleic acid sequencing
EP2307540B1 (en) 2008-07-07 2017-04-19 Oxford Nanopore Technologies Limited Enzyme-pore constructs
WO2010004273A1 (en) * 2008-07-07 2010-01-14 Oxford Nanopore Technologies Limited Base-detecting pore
CA2931824C (en) 2008-09-22 2020-09-08 University Of Washington Msp nanopores and related methods

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
David W. Deamer and Mark Akeson.Nanopores and nucleic acids: prospects for ultrarapid sequencing.《TIBTECH》.2000,第18卷(第4期),147-151. *
JOHN J. KASIANOWICZ et al..Characterization of individual polynucleotide molecules using a membrane channel.《Proceedings of the National Academy of Sciences of USA》.1996,第93卷(第24期),"INTRODUCTION"和"MATERIALS AND METHODS"部分,第13773页第5-10行. *
Mark Akeson et al..Microsecond Time-Scale Discrimination Among Polycytidylic Acid, Polyadenylic Acid, and Polyuridylic Acid as Homopolymers or as Segments Within Single RNA Molecules.《Biophysical Journal》.1999,第77卷(第6期),"INTRODUCTION"和"MATERIALS AND METHODS"部分,第3233页19-24行. *
Stefan Howorka et al..Sequence-specific detection of individual DNA strands using engineered nanopores.《nature biotechnology》.2001,第19卷(第7期),636-639. *
贾帅争等.纳米通道技术及应用.《生物化学与生物物理进展》.2002,第29卷(第2期),202-205. *

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EP1951898B1 (en) 2012-02-15
CA2629009A1 (en) 2007-05-24
IL191319A (en) 2011-10-31
NZ568163A (en) 2011-11-25
BRPI0618391A2 (pt) 2011-08-30
RU2008123842A (ru) 2009-12-27
EP2272981B1 (en) 2013-01-09
MY149161A (en) 2013-07-31
US20120219960A1 (en) 2012-08-30
US20080311582A1 (en) 2008-12-18
CA2629009C (en) 2016-02-02
ATE545712T1 (de) 2012-03-15
GB0523282D0 (en) 2005-12-21
WO2007057668A1 (en) 2007-05-24
AU2006314296B2 (en) 2012-08-23
UA95087C2 (en) 2011-07-11
KR20080083281A (ko) 2008-09-17
EP2272981A1 (en) 2011-01-12
JP5328362B2 (ja) 2013-10-30
US8785211B2 (en) 2014-07-22
US8105846B2 (en) 2012-01-31
EP1951898A1 (en) 2008-08-06
CN101356288A (zh) 2009-01-28
JP2009519705A (ja) 2009-05-21

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