IN2014DN00221A - - Google Patents

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Publication number
IN2014DN00221A
IN2014DN00221A IN221DEN2014A IN2014DN00221A IN 2014DN00221 A IN2014DN00221 A IN 2014DN00221A IN 221DEN2014 A IN221DEN2014 A IN 221DEN2014A IN 2014DN00221 A IN2014DN00221 A IN 2014DN00221A
Authority
IN
India
Prior art keywords
target polynucleotide
strands
double stranded
polynucleotide
stranded target
Prior art date
Application number
Other languages
English (en)
Inventor
Clive Brown
James Clarke
Graham Hall
Gavin Harper
Andrew Heron
James White
Original Assignee
Oxford Nanopore Tech Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Oxford Nanopore Tech Ltd filed Critical Oxford Nanopore Tech Ltd
Publication of IN2014DN00221A publication Critical patent/IN2014DN00221A/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44791—Microapparatus
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48721—Investigating individual macromolecules, e.g. by translocation through nanopores

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pathology (AREA)
  • Electrochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Dispersion Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
IN221DEN2014 2011-07-25 2012-07-25 IN2014DN00221A (enExample)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161511436P 2011-07-25 2011-07-25
PCT/GB2012/051786 WO2013014451A1 (en) 2011-07-25 2012-07-25 Hairpin loop method for double strand polynucleotide sequencing using transmembrane pores

Publications (1)

Publication Number Publication Date
IN2014DN00221A true IN2014DN00221A (enExample) 2015-06-05

Family

ID=46763140

Family Applications (1)

Application Number Title Priority Date Filing Date
IN221DEN2014 IN2014DN00221A (enExample) 2011-07-25 2012-07-25

Country Status (10)

Country Link
US (8) US9957560B2 (enExample)
EP (1) EP2737084B1 (enExample)
JP (1) JP6298404B2 (enExample)
KR (1) KR20140050067A (enExample)
CN (1) CN103827320B (enExample)
AU (1) AU2012288629B2 (enExample)
BR (1) BR112014001699A2 (enExample)
CA (1) CA2843136C (enExample)
IN (1) IN2014DN00221A (enExample)
WO (1) WO2013014451A1 (enExample)

Families Citing this family (100)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE202009019021U1 (de) 2008-09-22 2015-09-24 University Of Washington Msp-nanoporen
EP2391732B1 (en) 2009-01-30 2015-05-27 Oxford Nanopore Technologies Limited Methods using adaptors for nucleic acid constructs in transmembrane sequencing
SG10201604316WA (en) 2011-05-27 2016-07-28 Oxford Nanopore Tech Ltd Coupling method
KR20140050067A (ko) 2011-07-25 2014-04-28 옥스포드 나노포어 테크놀로지즈 리미티드 막횡단 포어를 사용한 이중 가닥 폴리뉴클레오티드 서열분석을 위한 헤어핀 루프 방법
EP3663412B1 (en) 2011-09-23 2022-03-09 Oxford Nanopore Technologies PLC Analysis of a polymer comprising polymer units by means of translocation through a nanopore
CN104039979B (zh) 2011-10-21 2016-08-24 牛津纳米孔技术公司 使用孔和Hel308解旋酶表征目标多核苷酸的酶方法
EP2798083B1 (en) 2011-12-29 2017-08-09 Oxford Nanopore Technologies Limited Method for characterising a polynucelotide by using a xpd helicase
CA2861808C (en) 2011-12-29 2021-02-23 Oxford Nanopore Technologies Limited Enzyme method
US9238836B2 (en) 2012-03-30 2016-01-19 Pacific Biosciences Of California, Inc. Methods and compositions for sequencing modified nucleic acids
CA2864035C (en) 2012-02-15 2021-05-18 Oxford Nanopore Technologies Limited A method for determining the presence of an analyte using an aptamer
US9850534B2 (en) 2012-02-16 2017-12-26 Genia Technologies, Inc. Methods for creating bilayers for use with nanopore sensors
EP3736339B1 (en) 2012-02-16 2022-07-27 Oxford Nanopore Technologies plc Analysis of measurements of a polymer
WO2013153359A1 (en) 2012-04-10 2013-10-17 Oxford Nanopore Technologies Limited Mutant lysenin pores
GB2559073A (en) 2012-06-08 2018-07-25 Pacific Biosciences California Inc Modified base detection with nanopore sequencing
CA2879355C (en) 2012-07-19 2021-09-21 Oxford Nanopore Technologies Limited Helicase construct and its use in characterising polynucleotides
AU2013291763B2 (en) 2012-07-19 2019-06-20 Oxford Nanopore Technologies Limited Modified helicases
US11155860B2 (en) 2012-07-19 2021-10-26 Oxford Nanopore Technologies Ltd. SSB method
WO2014041337A1 (en) * 2012-09-14 2014-03-20 Oxford Nanopore Technologies Limited Sample preparation method
WO2014072703A1 (en) 2012-11-06 2014-05-15 Oxford Nanopore Technologies Limited Quadruplex method
WO2014074922A1 (en) * 2012-11-09 2014-05-15 Stratos Genomics, Inc. Concentrating a target molecule for sensing by a nanopore
GB201222928D0 (en) 2012-12-19 2013-01-30 Oxford Nanopore Tech Ltd Analysis of a polynucleotide
AU2014218911B2 (en) 2013-02-20 2018-07-12 Eve Biomedical, Inc. Methods and compositions for nanostructure-based nucleic acid sequencing
GB201314695D0 (en) 2013-08-16 2013-10-02 Oxford Nanopore Tech Ltd Method
GB201318465D0 (en) 2013-10-18 2013-12-04 Oxford Nanopore Tech Ltd Method
CA2901545C (en) 2013-03-08 2019-10-08 Oxford Nanopore Technologies Limited Use of spacer elements in a nucleic acid to control movement of a helicase
GB201313477D0 (en) 2013-07-29 2013-09-11 Univ Leuven Kath Nanopore biosensors for detection of proteins and nucleic acids
EP3575410A3 (en) 2013-10-18 2020-03-04 Oxford Nanopore Technologies Limited Modified enzymes
GB201406151D0 (en) 2014-04-04 2014-05-21 Oxford Nanopore Tech Ltd Method
GB201406155D0 (en) 2014-04-04 2014-05-21 Oxford Nanopore Tech Ltd Method
EP3097210B2 (en) 2014-01-22 2022-04-20 Oxford Nanopore Technologies Limited Method for attaching one or more polynucleotide binding proteins to a target polynucleotide
US10359395B2 (en) 2014-02-19 2019-07-23 University Of Washington Nanopore-based analysis of protein characteristics
GB201403096D0 (en) * 2014-02-21 2014-04-09 Oxford Nanopore Tech Ltd Sample preparation method
US10337060B2 (en) 2014-04-04 2019-07-02 Oxford Nanopore Technologies Ltd. Method for characterising a double stranded nucleic acid using a nano-pore and anchor molecules at both ends of said nucleic acid
US10443097B2 (en) 2014-05-02 2019-10-15 Oxford Nanopore Technologies Ltd. Method of improving the movement of a target polynucleotide with respect to a transmembrane pore
GB201417712D0 (en) * 2014-10-07 2014-11-19 Oxford Nanopore Tech Ltd Method
WO2016009180A1 (en) 2014-07-14 2016-01-21 Isis Innovation Limited Measurement of analytes with membrane channel molecules, and bilayer arrays
CN119899249A (zh) 2014-09-01 2025-04-29 弗拉芒区生物技术研究所 突变csgg孔
US10421998B2 (en) 2014-09-29 2019-09-24 The Regents Of The University Of California Nanopore sequencing of polynucleotides with multiple passes
US10266885B2 (en) 2014-10-07 2019-04-23 Oxford Nanopore Technologies Ltd. Mutant pores
GB201418159D0 (en) 2014-10-14 2014-11-26 Oxford Nanopore Tech Ltd Method
EP3971300B1 (en) 2014-10-16 2024-06-05 Oxford Nanopore Technologies plc Sorting of polymers
WO2016059436A1 (en) 2014-10-17 2016-04-21 Oxford Nanopore Technologies Limited Method for nanopore rna characterisation
GB201418469D0 (en) * 2014-10-17 2014-12-03 Oxford Nanopore Tech Ltd Method
WO2016077313A1 (en) 2014-11-11 2016-05-19 Bgi Shenzhen Multi-pass sequencing
KR20190127983A (ko) 2015-02-02 2019-11-13 투 포어 가이즈, 인코포레이티드 시료 배경으로부터의 표적 폴리뉴클레오티드의 나노포어 검출
GB201502809D0 (en) 2015-02-19 2015-04-08 Oxford Nanopore Tech Ltd Mutant pore
GB201502810D0 (en) 2015-02-19 2015-04-08 Oxford Nanopore Tech Ltd Method
JP6560336B2 (ja) * 2015-02-26 2019-08-14 株式会社日立ハイテクノロジーズ 核酸分子の構築方法
CN107735686B (zh) 2015-04-14 2021-06-11 鲁汶天主教大学 具有内部蛋白质衔接子的纳米孔
US11319586B2 (en) * 2015-08-12 2022-05-03 The Chinese University Of Hong Kong Single-molecule sequencing of plasma DNA
US10280411B2 (en) 2015-10-27 2019-05-07 Pacific Biosciences of California, In.c Methods, systems, and reagents for direct RNA sequencing
CN109072295B (zh) 2015-12-08 2022-05-31 鲁汶大学研究与发展中心 修饰的纳米孔,包含其的组合物及其应用
WO2017100027A1 (en) 2015-12-08 2017-06-15 Quantapore, Inc. Method of translocating nucleic acids through nanopores
KR102222191B1 (ko) 2016-03-02 2021-03-02 옥스포드 나노포어 테크놀로지즈 리미티드 돌연변이체 기공
US10961573B2 (en) 2016-03-28 2021-03-30 Boreal Genomics, Inc. Linked duplex target capture
CN118272512A (zh) 2016-04-06 2024-07-02 牛津纳米孔科技公开有限公司 突变体孔
GB201609220D0 (en) 2016-05-25 2016-07-06 Oxford Nanopore Tech Ltd Method
EP3464616B1 (en) 2016-05-25 2022-05-04 Oxford Nanopore Technologies plc Method
GB201609221D0 (en) 2016-05-25 2016-07-06 Oxford Nanopore Tech Ltd Method
WO2017209891A1 (en) 2016-05-31 2017-12-07 Quantapore, Inc. Two-color nanopore sequencing
CN109416354B (zh) 2016-06-27 2020-07-10 豪夫迈·罗氏有限公司 用于双层形成的渗透不平衡方法
US10577653B2 (en) * 2016-06-27 2020-03-03 Roche Sequencing Solutions, Inc. Counteracting osmotic imbalance in a sequencing cell
GB201612458D0 (en) * 2016-07-14 2016-08-31 Howorka Stefan And Pugh Genevieve Membrane spanning DNA nanopores for molecular transport
CN109804087A (zh) 2016-08-19 2019-05-24 昆塔波尔公司 使用猝灭剂的基于光学的纳米孔测序
GB201617886D0 (en) * 2016-10-21 2016-12-07 Oxford Nanopore Technologies Limited Method
GB201620450D0 (en) 2016-12-01 2017-01-18 Oxford Nanopore Tech Ltd Method
JP7048609B2 (ja) 2016-12-09 2022-04-05 ボリアル ジェノミクス, インコーポレイテッド 連結型ライゲーション
CA3053086A1 (en) 2017-02-10 2018-08-16 Oxford Nanopore Technologies Limited Modified nanopores, compositions comprising the same, and uses thereof
GB201707140D0 (en) 2017-05-04 2017-06-21 Oxford Nanopore Tech Ltd Method
GB201707138D0 (en) 2017-05-04 2017-06-21 Oxford Nanopore Tech Ltd Machine learning analysis of nanopore measurements
GB201707122D0 (en) 2017-05-04 2017-06-21 Oxford Nanopore Tech Ltd Pore
JP7038147B2 (ja) * 2017-06-21 2022-03-17 ヌーマ バイオ インコーポレイテッド 二重細孔制御およびセンサデバイス
EP3645552B1 (en) 2017-06-30 2023-06-28 Vib Vzw Novel protein pores
EP3731959B1 (en) 2017-12-29 2025-10-08 Clear Labs, Inc. Automated priming and library loading device
WO2019183640A1 (en) * 2018-03-23 2019-09-26 Board Of Regents, The University Of Texas System Efficient sequencing of dsdna with extremely low level of errors
GB201807793D0 (en) 2018-05-14 2018-06-27 Oxford Nanopore Tech Ltd Method
GB201809323D0 (en) 2018-06-06 2018-07-25 Oxford Nanopore Tech Ltd Method
WO2020002621A2 (en) 2018-06-29 2020-01-02 F. Hoffmann-La Roche Ag Detection of microsatellite instability
US12098419B2 (en) 2018-08-23 2024-09-24 Ncan Genomics, Inc. Linked target capture and ligation
GB201814369D0 (en) 2018-09-04 2018-10-17 Oxford Nanopore Tech Ltd Method for determining a polymersequence
EP3650558A1 (en) * 2018-11-07 2020-05-13 Siemens Healthcare GmbH Liquid sample workflow for nanopore sequencing
WO2020106630A1 (en) * 2018-11-19 2020-05-28 The Regents Of The University Of California Methods for detecting and sequencing a target nucleic acid
GB201819378D0 (en) 2018-11-28 2019-01-09 Oxford Nanopore Tech Ltd Analysis of nanopore signal using a machine-learning technique
EP4538389A3 (en) 2019-01-03 2025-07-30 Ncan Genomics, Inc. Linked target capture
WO2020168286A1 (en) 2019-02-14 2020-08-20 University Of Washington Systems and methods for improved nanopore-based analysis of nucleic acids
US11860123B2 (en) 2019-04-16 2024-01-02 The Board Of Trustees Of The University Of Illinois Classification of epigenetic biomarkers and/or DNA conformational superstructures via use of atomically thin nanopores
GB201917060D0 (en) * 2019-11-22 2020-01-08 Oxford Nanopore Tech Ltd Method
EP4114966B1 (en) 2020-03-03 2025-02-26 Pacific Biosciences Of California, Inc. Methods and compositions for sequencing double stranded nucleic acids
EP4168582B1 (en) 2020-06-18 2025-11-19 Oxford Nanopore Technologies plc A method of selectively characterising a polynucleotide using a detector
MX2023001142A (es) 2020-07-30 2023-05-25 Cambridge Epigenetix Ltd Composiciones y metodos para analisis de acidos nucleicos.
US11248265B1 (en) 2020-11-19 2022-02-15 Clear Labs, Inc Systems and processes for distinguishing pathogenic and non-pathogenic sequences from specimens
CN115029424B (zh) * 2021-03-03 2025-05-16 郑州思昆生物工程有限公司 一种固定化模板多核苷酸配对末端测序方法
CN113462764B (zh) * 2021-09-01 2021-12-17 北京齐碳科技有限公司 用于表征双链靶多核苷酸的类发夹衔接子、构建体和方法
CN114134142B (zh) * 2021-11-23 2024-05-28 成都齐碳科技有限公司 衔接体、复合物、单链分子、试剂盒、方法和应用
CN114262735B (zh) * 2021-12-15 2025-09-16 成都齐碳科技有限公司 用于表征多核苷酸的衔接体及其用途
CN114457145B (zh) * 2022-01-29 2023-08-11 成都齐碳科技有限公司 用于表征靶多核苷酸测序的接头、构建体、方法和应用
CN120435657A (zh) 2022-10-28 2025-08-05 格罗宁根大学 蛋白质的基于纳米孔的分析
WO2024124567A1 (zh) * 2022-12-16 2024-06-20 深圳华大生命科学研究院 纳米孔测序方法和试剂盒
WO2024138572A1 (zh) * 2022-12-29 2024-07-04 深圳华大生命科学研究院 测序接头、测序接头复合物、靶核酸序列多次纳米孔测序的方法
GB202414276D0 (en) 2024-09-27 2024-11-13 Oxford Nanopore Tech Plc Method

Family Cites Families (196)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FI82266C (fi) 1982-10-19 1991-02-11 Cetus Corp Foerfarande foer framstaellning av il-2 -mutein.
GB8924338D0 (en) 1989-10-28 1989-12-13 Atomic Energy Authority Uk Electrodes
US5215899A (en) 1989-11-09 1993-06-01 Miles Inc. Nucleic acid amplification employing ligatable hairpin probe and transcription
US5424413A (en) 1992-01-22 1995-06-13 Gen-Probe Incorporated Branched nucleic acid probes
FR2703693B1 (fr) 1993-04-06 1995-07-13 Pasteur Institut Procédé rapide de détermination d'une séquence d'ADN et application au séquençage et au diagnostic.
US5714320A (en) 1993-04-15 1998-02-03 University Of Rochester Rolling circle synthesis of oligonucleotides and amplification of select randomized circular oligonucleotides
US5777078A (en) 1993-04-28 1998-07-07 Worcester Foundation For Experimental Biology Triggered pore-forming agents
EP0753071A1 (en) 1993-04-28 1997-01-15 Worcester Foundation For Experimental Biology Cell-targeted lytic pore-forming agents
DE4320201A1 (de) 1993-06-18 1995-01-12 Asta Medica Ag Verwendung von Cetrorelix und weiteren Nona- und Dekapeptiden zur Herstellung eines Arzneimittels zur Bekämpfung von Aids und zur Wachstumsstimulation
US5561043A (en) 1994-01-31 1996-10-01 Trustees Of Boston University Self-assembling multimeric nucleic acid constructs
US7569341B2 (en) 1994-01-31 2009-08-04 Trustees Of Boston University Nucleic acid directed immobilization arrays and methods of assembly
US6362002B1 (en) 1995-03-17 2002-03-26 President And Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
US5795782A (en) 1995-03-17 1998-08-18 President & Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
US6395887B1 (en) 1995-08-01 2002-05-28 Yale University Analysis of gene expression by display of 3'-end fragments of CDNAS
US5866336A (en) 1996-07-16 1999-02-02 Oncor, Inc. Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
DE19648625A1 (de) 1996-11-13 1998-05-14 Soft Gene Gmbh Mikroprojektil für das Einbringen von Substanzen in Zellen durch ballistischen Transfer
WO1999005167A1 (en) 1997-07-25 1999-02-04 University Of Massachusetts Designed protein pores as components for biosensors
US6087099A (en) 1997-09-08 2000-07-11 Myriad Genetics, Inc. Method for sequencing both strands of a double stranded DNA in a single sequencing reaction
US6127166A (en) 1997-11-03 2000-10-03 Bayley; Hagan Molluscan ligament polypeptides and genes encoding them
JPH11137260A (ja) 1997-11-06 1999-05-25 Soyaku Gijutsu Kenkyusho:Kk 抗インフルエンザウイルス環状ダンベル型rna−dnaキメラ化合物及び抗インフルエンザウイルス剤
US6123819A (en) 1997-11-12 2000-09-26 Protiveris, Inc. Nanoelectrode arrays
DE19826758C1 (de) 1998-06-15 1999-10-21 Soft Gene Gmbh Darstellung von linearen kovalent geschlossenen DNA-Molekülen als Expressionskonstrukte
US6743605B1 (en) 1998-06-24 2004-06-01 Enzo Life Sciences, Inc. Linear amplification of specific nucleic acid sequences
US6787308B2 (en) * 1998-07-30 2004-09-07 Solexa Ltd. Arrayed biomolecules and their use in sequencing
US6235502B1 (en) 1998-09-18 2001-05-22 Molecular Staging Inc. Methods for selectively isolating DNA using rolling circle amplification
US6267872B1 (en) 1998-11-06 2001-07-31 The Regents Of The University Of California Miniature support for thin films containing single channels or nanopores and methods for using same
US6426231B1 (en) 1998-11-18 2002-07-30 The Texas A&M University System Analyte sensing mediated by adapter/carrier molecules
AU2206800A (en) 1998-12-11 2000-06-26 Regents Of The University Of California, The Targeted molecular bar codes and methods for using the same
NO986133D0 (no) 1998-12-23 1998-12-23 Preben Lexow FremgangsmÕte for DNA-sekvensering
US7056661B2 (en) 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
EP1192103A1 (en) * 1999-06-22 2002-04-03 President And Fellows of Harvard College Control of solid state dimensional features
CA2376650C (en) 1999-06-29 2008-01-15 University Health Network Peptide conjugates for the stabilization of membrane proteins and interactions with biological membranes
ATE318932T1 (de) 1999-08-13 2006-03-15 Univ Yale Binär kodierte sequenzmarker
US6274320B1 (en) 1999-09-16 2001-08-14 Curagen Corporation Method of sequencing a nucleic acid
US6682649B1 (en) 1999-10-01 2004-01-27 Sophion Bioscience A/S Substrate and a method for determining and/or monitoring electrophysiological properties of ion channels
EP1238105A2 (en) 1999-12-02 2002-09-11 Molecular Staging Inc. Generation of single-strand circular dna from linear self-annealing segments
US6916665B2 (en) 2000-02-11 2005-07-12 The Texas A&M University System Biosensor compositions and methods of use
AU2001250912B2 (en) 2000-03-21 2007-01-04 Curagen Corporation VEGF-modulated genes and methods employing them
JP2003527849A (ja) 2000-03-22 2003-09-24 キュラゲン コーポレイション 新規ペプチド及びこれをコードする核酸
US6596488B2 (en) 2000-03-30 2003-07-22 City Of Hope Tumor suppressor gene
US6387624B1 (en) 2000-04-14 2002-05-14 Incyte Pharmaceuticals, Inc. Construction of uni-directionally cloned cDNA libraries from messenger RNA for improved 3′ end DNA sequencing
US7001792B2 (en) 2000-04-24 2006-02-21 Eagle Research & Development, Llc Ultra-fast nucleic acid sequencing device and a method for making and using the same
US20020132350A1 (en) 2000-09-14 2002-09-19 Pioneer Hi-Bred International, Inc. Targeted genetic manipulation using Mu bacteriophage cleaved donor complex
US6709861B2 (en) 2000-11-17 2004-03-23 Lucigen Corp. Cloning vectors and vector components
AU2002239284A1 (en) 2000-11-27 2002-06-03 The Regents Of The University Of California Methods and devices for characterizing duplex nucleic acid molecules
US20020197618A1 (en) 2001-01-20 2002-12-26 Sampson Jeffrey R. Synthesis and amplification of unstructured nucleic acids for rapid sequencing
US20030087232A1 (en) 2001-01-25 2003-05-08 Fred Christians Methods for screening polypeptides
US7807408B2 (en) 2001-03-19 2010-10-05 President & Fellows Of Harvard College Directed evolution of proteins
US6863833B1 (en) 2001-06-29 2005-03-08 The Board Of Trustees Of The Leland Stanford Junior University Microfabricated apertures for supporting bilayer lipid membranes
WO2003004992A2 (en) 2001-07-03 2003-01-16 The Regents Of The University Of California Mammalian sweet and amino acid heterodimeric taste receptors
US6852492B2 (en) 2001-09-24 2005-02-08 Intel Corporation Nucleic acid sequencing by raman monitoring of uptake of precursors during molecular replication
DE60324778D1 (de) 2002-03-15 2009-01-02 Nuevolution As Eine verbesserte methode zur synthese von matritzenabhängigen molekülen
WO2003095669A1 (en) 2002-05-10 2003-11-20 The Texas A & M University System Stochastic sensing through covalent interactions
US7452699B2 (en) 2003-01-15 2008-11-18 Dana-Farber Cancer Institute, Inc. Amplification of DNA in a hairpin structure, and applications
CA2515938A1 (en) 2003-02-12 2004-08-26 Genizon Svenska Ab Methods and means for nucleic acid sequencing
US20100035254A1 (en) 2003-04-08 2010-02-11 Pacific Biosciences Of California, Inc. Composition and method for nucleic acid sequencing
US7745116B2 (en) 2003-04-08 2010-06-29 Pacific Biosciences Of California, Inc. Composition and method for nucleic acid sequencing
US7163658B2 (en) 2003-04-23 2007-01-16 Rouvain Bension Rapid sequencing of polymers
US7344882B2 (en) 2003-05-12 2008-03-18 Bristol-Myers Squibb Company Polynucleotides encoding variants of the TRP channel family member, LTRPC3
WO2005056750A2 (en) 2003-12-11 2005-06-23 Quark Biotech, Inc. Inversion-duplication of nucleic acids and libraries prepared thereby
GB0400584D0 (en) 2004-01-12 2004-02-11 Solexa Ltd Nucleic acid chacterisation
WO2006028508A2 (en) 2004-03-23 2006-03-16 President And Fellows Of Harvard College Methods and apparatus for characterizing polynucleotides
GB2413796B (en) 2004-03-25 2006-03-29 Global Genomics Ab Methods and means for nucleic acid sequencing
US20050227239A1 (en) * 2004-04-08 2005-10-13 Joyce Timothy H Microarray based affinity purification and analysis device coupled with solid state nanopore electrodes
WO2005118877A2 (en) 2004-06-02 2005-12-15 Vicus Bioscience, Llc Producing, cataloging and classifying sequence tags
WO2005124888A1 (en) 2004-06-08 2005-12-29 President And Fellows Of Harvard College Suspended carbon nanotube field effect transistor
US7700281B2 (en) 2004-06-30 2010-04-20 Usb Corporation Hot start nucleic acid amplification
WO2006020775A2 (en) * 2004-08-13 2006-02-23 Harvard University An ultra high-throughput opti-nanopore dna readout platform
US20060086626A1 (en) * 2004-10-22 2006-04-27 Joyce Timothy H Nanostructure resonant tunneling with a gate voltage source
US7867716B2 (en) 2004-12-21 2011-01-11 The Texas A&M University System High temperature ion channels and pores
US7890268B2 (en) 2004-12-28 2011-02-15 Roche Molecular Systems, Inc. De-novo sequencing of nucleic acids
GB0505971D0 (en) 2005-03-23 2005-04-27 Isis Innovation Delivery of molecules to a lipid bilayer
US7507575B2 (en) 2005-04-01 2009-03-24 3M Innovative Properties Company Multiplex fluorescence detection device having removable optical modules
WO2007145612A1 (en) * 2005-06-06 2007-12-21 454 Life Sciences Corporation Paired end sequencing
US20070020640A1 (en) 2005-07-21 2007-01-25 Mccloskey Megan L Molecular encoding of nucleic acid templates for PCR and other forms of sequence analysis
DK1924704T3 (da) 2005-08-02 2011-09-05 Rubicon Genomics Inc Sammensætninger og fremgangsmåder til bearbejdning og mangfoldiggørelse af DNA, herunder ved anvendelse af flere enzymer i en enkelt reaktion
WO2007024997A2 (en) 2005-08-22 2007-03-01 Fermalogic, Inc. Methods of increasing production of secondary metabolites
GB0523282D0 (en) 2005-11-15 2005-12-21 Isis Innovation Methods using pores
AU2006331512B2 (en) 2005-12-22 2012-02-23 Pacific Biosciences Of California, Inc. Active surface coupled polymerases
US7932029B1 (en) 2006-01-04 2011-04-26 Si Lok Methods for nucleic acid mapping and identification of fine-structural-variations in nucleic acids and utilities
WO2007098427A2 (en) * 2006-02-18 2007-08-30 Michael Strathmann Massively multiplexed sequencing
US8673567B2 (en) * 2006-03-08 2014-03-18 Atila Biosystems, Inc. Method and kit for nucleic acid sequence detection
EP2002017B1 (en) 2006-04-04 2015-06-10 Keygene N.V. High throughput detection of molecular markers based on restriction fragments
CN101495656B (zh) 2006-06-07 2017-02-08 纽约哥伦比亚大学理事会 采用带修饰的核苷酸通过纳米通道进行dna序列测定
JP4876766B2 (ja) 2006-08-10 2012-02-15 トヨタ自動車株式会社 燃料電池
CN101578373A (zh) 2006-09-06 2009-11-11 费斯生物制药公司 融合肽治疗组合物
US20100311602A1 (en) 2006-10-13 2010-12-09 J. Craig Venter Institute, Inc. Sequencing method
EP2089517A4 (en) 2006-10-23 2010-10-20 Pacific Biosciences California POLYMERASEENZYME AND REAGENTS FOR ADVANCED NUCKIC ACID SEQUENCING
GB2445016B (en) 2006-12-19 2012-03-07 Microsaic Systems Plc Microengineered ionisation device
EP2122344B8 (en) 2007-02-20 2019-08-21 Oxford Nanopore Technologies Limited Lipid bilayer sensor system
WO2008124107A1 (en) 2007-04-04 2008-10-16 The Regents Of The University Of California Compositions, devices, systems, and methods for using a nanopore
EP3540436B1 (en) 2007-09-12 2023-11-01 President And Fellows Of Harvard College High-resolution molecular sensor
GB2453377A (en) 2007-10-05 2009-04-08 Isis Innovation Transmembrane protein pores and molecular adapters therefore.
KR101414713B1 (ko) 2007-10-11 2014-07-03 삼성전자주식회사 리가제 및 엔도뉴클레아제의 존재하에서 롤링서클 증폭에의하여 표적 핵산을 증폭하는 방법
CN100554946C (zh) * 2007-12-18 2009-10-28 中国农业大学 一种双链核酸精确定量的方法
GB0724736D0 (en) 2007-12-19 2008-01-30 Oxford Nanolabs Ltd Formation of layers of amphiphilic molecules
US8502972B2 (en) 2007-12-31 2013-08-06 Fujirebio Inc. Clusters of microresonators for cavity mode optical sensing
WO2009092035A2 (en) * 2008-01-17 2009-07-23 Sequenom, Inc. Methods and compositions for the analysis of biological molecules
US8263367B2 (en) 2008-01-25 2012-09-11 Agency For Science, Technology And Research Nucleic acid interaction analysis
US8231969B2 (en) 2008-03-26 2012-07-31 University Of Utah Research Foundation Asymmetrically functionalized nanoparticles
US8628940B2 (en) 2008-09-24 2014-01-14 Pacific Biosciences Of California, Inc. Intermittent detection during analytical reactions
JP2011515102A (ja) 2008-03-28 2011-05-19 パシフィック バイオサイエンシーズ オブ カリフォルニア, インコーポレイテッド 核酸シーケンシング用組成物及び方法
US8143030B2 (en) 2008-09-24 2012-03-27 Pacific Biosciences Of California, Inc. Intermittent detection during analytical reactions
WO2009120374A2 (en) 2008-03-28 2009-10-01 Pacific Biosciences Of California, Inc. Methods and compositions for nucleic acid sample preparation
WO2009132124A2 (en) 2008-04-24 2009-10-29 The Trustees Of Columbia University In The City Of New York Geometric patterns and lipid bilayers for dna molecule organization and uses thereof
US20090269771A1 (en) * 2008-04-24 2009-10-29 Life Technologies Corporation Method of sequencing and mapping target nucleic acids
US20110229877A1 (en) 2008-07-07 2011-09-22 Oxford Nanopore Technologies Limited Enzyme-pore constructs
CA2730068A1 (en) 2008-07-07 2010-01-14 Oxford Nanopore Technologies Limited Base-detecting pore
US20100092960A1 (en) 2008-07-25 2010-04-15 Pacific Biosciences Of California, Inc. Helicase-assisted sequencing with molecular beacons
WO2010030683A1 (en) * 2008-09-09 2010-03-18 Rosetta Inpharmatics Llc Methods of generating gene specific libraries
US8481264B2 (en) 2008-09-19 2013-07-09 Pacific Biosciences Of California, Inc. Immobilized nucleic acid complexes for sequence analysis
DE202009019021U1 (de) 2008-09-22 2015-09-24 University Of Washington Msp-nanoporen
US8383369B2 (en) 2008-09-24 2013-02-26 Pacific Biosciences Of California, Inc. Intermittent detection during analytical reactions
US9080211B2 (en) 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
EP2664678B1 (en) 2008-10-24 2014-10-08 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
US8486630B2 (en) * 2008-11-07 2013-07-16 Industrial Technology Research Institute Methods for accurate sequence data and modified base position determination
GB0820927D0 (en) 2008-11-14 2008-12-24 Isis Innovation Method
AU2009325069B2 (en) * 2008-12-11 2015-03-19 Pacific Biosciences Of California, Inc. Classification of nucleic acid templates
CN102482330A (zh) 2009-01-30 2012-05-30 牛津纳米孔技术有限公司 酶突变体
EP2391732B1 (en) 2009-01-30 2015-05-27 Oxford Nanopore Technologies Limited Methods using adaptors for nucleic acid constructs in transmembrane sequencing
AU2010213510B2 (en) 2009-02-16 2014-05-01 Epicentre Technologies Corporation Template-independent ligation of single-stranded DNA
US8399219B2 (en) 2009-02-23 2013-03-19 Cytomx Therapeutics, Inc. Protease activatable interferon alpha proprotein
GB0905140D0 (en) 2009-03-25 2009-05-06 Isis Innovation Method
FR2943656A1 (fr) 2009-03-25 2010-10-01 Air Liquide Procede et installation de production d'hydrogene mettant en oeuvre un compresseur thermocinetique
WO2010117470A2 (en) 2009-04-10 2010-10-14 Pacific Biosciences Of California, Inc. Nanopore sequencing devices and methods
CA2758617C (en) 2009-04-20 2017-10-03 Oxford Nanopore Technologies Limited Lipid bilayer sensor array
GB0910302D0 (en) 2009-06-15 2009-07-29 Lumora Ltd Nucleic acid amplification
US20120015821A1 (en) 2009-09-09 2012-01-19 Life Technologies Corporation Methods of Generating Gene Specific Libraries
EP2507387B1 (en) 2009-12-01 2017-01-25 Oxford Nanopore Technologies Limited Biochemical analysis instrument and method
US20120010085A1 (en) * 2010-01-19 2012-01-12 Rava Richard P Methods for determining fraction of fetal nucleic acids in maternal samples
FR2955773B1 (fr) 2010-02-01 2017-05-26 Commissariat Energie Atomique Complexe moleculaire de ciblage des antigenes vers les cellules presentatrices d'antigene et ses applications pour la vaccination
KR20110100963A (ko) * 2010-03-05 2011-09-15 삼성전자주식회사 미세 유동 장치 및 이를 이용한 표적 핵산의 염기 서열 결정 방법
US20110224106A1 (en) 2010-03-10 2011-09-15 Ibis Biosciences, Inc. Production Of Single-Stranded Circular Nucleic Acid
US8652779B2 (en) 2010-04-09 2014-02-18 Pacific Biosciences Of California, Inc. Nanopore sequencing using charge blockade labels
US20120244525A1 (en) 2010-07-19 2012-09-27 New England Biolabs, Inc. Oligonucleotide Adapters: Compositions and Methods of Use
EP2614156B1 (en) 2010-09-07 2018-08-01 The Regents of The University of California Control of dna movement in a nanopore at one nucleotide precision by a processive enzyme
CA2821299C (en) 2010-11-05 2019-02-12 Frank J. Steemers Linking sequence reads using paired code tags
CN103282518B (zh) 2010-12-17 2016-11-16 纽约哥伦比亚大学理事会 使用经修饰的核苷酸和纳米孔检测的dna边合成边测序
US20130291392A1 (en) 2011-01-18 2013-11-07 R.K. Swamy Multipurpose instrument for triangle solutions, measurements and geometrical applications called triometer
US9402808B2 (en) 2011-01-19 2016-08-02 Panacea Biotec Limited Liquid oral composition of lanthanum salts
EP2668273B9 (en) 2011-01-28 2017-03-29 Illumina, Inc. Oligonucleotide replacement for di-tagged and directional libraries
US20120196279A1 (en) 2011-02-02 2012-08-02 Pacific Biosciences Of California, Inc. Methods and compositions for nucleic acid sample preparation
BR112013020411B1 (pt) 2011-02-11 2021-09-08 Oxford Nanopore Technologies Limited Monômero de msp mutante, construto, polinucleotídeo, poro, kit e aparelho para caracterizar uma sequência de ácido nucleico alvo, e, método para caracterizar uma sequência de ácido nucleico alvo
US9347929B2 (en) 2011-03-01 2016-05-24 The Regents Of The University Of Michigan Controlling translocation through nanopores with fluid wall
SG10201604316WA (en) 2011-05-27 2016-07-28 Oxford Nanopore Tech Ltd Coupling method
US20130017978A1 (en) 2011-07-11 2013-01-17 Finnzymes Oy Methods and transposon nucleic acids for generating a dna library
US9145623B2 (en) 2011-07-20 2015-09-29 Thermo Fisher Scientific Oy Transposon nucleic acids comprising a calibration sequence for DNA sequencing
KR20140050067A (ko) 2011-07-25 2014-04-28 옥스포드 나노포어 테크놀로지즈 리미티드 막횡단 포어를 사용한 이중 가닥 폴리뉴클레오티드 서열분석을 위한 헤어핀 루프 방법
US9632102B2 (en) 2011-09-25 2017-04-25 Theranos, Inc. Systems and methods for multi-purpose analysis
EP3663412B1 (en) 2011-09-23 2022-03-09 Oxford Nanopore Technologies PLC Analysis of a polymer comprising polymer units by means of translocation through a nanopore
US20140308661A1 (en) 2011-09-25 2014-10-16 Theranos, Inc. Systems and methods for multi-analysis
US9810704B2 (en) 2013-02-18 2017-11-07 Theranos, Inc. Systems and methods for multi-analysis
CN104039979B (zh) 2011-10-21 2016-08-24 牛津纳米孔技术公司 使用孔和Hel308解旋酶表征目标多核苷酸的酶方法
US9404147B2 (en) 2011-12-19 2016-08-02 Gen-Probe Incorporated Closed nucleic acid structures
EP2798083B1 (en) 2011-12-29 2017-08-09 Oxford Nanopore Technologies Limited Method for characterising a polynucelotide by using a xpd helicase
CA2861808C (en) 2011-12-29 2021-02-23 Oxford Nanopore Technologies Limited Enzyme method
NO2694769T3 (enExample) 2012-03-06 2018-03-03
WO2013153359A1 (en) 2012-04-10 2013-10-17 Oxford Nanopore Technologies Limited Mutant lysenin pores
GB2559073A (en) 2012-06-08 2018-07-25 Pacific Biosciences California Inc Modified base detection with nanopore sequencing
TWI655213B (zh) 2012-07-13 2019-04-01 Menicon Co., Ltd. 自我組織化肽衍生物的製造方法
AU2013291763B2 (en) 2012-07-19 2019-06-20 Oxford Nanopore Technologies Limited Modified helicases
CA2879355C (en) 2012-07-19 2021-09-21 Oxford Nanopore Technologies Limited Helicase construct and its use in characterising polynucleotides
US11155860B2 (en) 2012-07-19 2021-10-26 Oxford Nanopore Technologies Ltd. SSB method
WO2014041337A1 (en) 2012-09-14 2014-03-20 Oxford Nanopore Technologies Limited Sample preparation method
JP6375301B2 (ja) 2012-10-26 2018-08-15 オックスフォード ナノポール テクノロジーズ リミテッド 液滴界面
GB201313121D0 (en) 2013-07-23 2013-09-04 Oxford Nanopore Tech Ltd Array of volumes of polar medium
WO2014074922A1 (en) 2012-11-09 2014-05-15 Stratos Genomics, Inc. Concentrating a target molecule for sensing by a nanopore
US9683230B2 (en) 2013-01-09 2017-06-20 Illumina Cambridge Limited Sample preparation on a solid support
US20140206842A1 (en) 2013-01-22 2014-07-24 Muhammed Majeed Peptides Modified with Triterpenoids and Small Organic Molecules: Synthesis and use in Cosmeceutical
CA2901545C (en) 2013-03-08 2019-10-08 Oxford Nanopore Technologies Limited Use of spacer elements in a nucleic acid to control movement of a helicase
GB201318465D0 (en) 2013-10-18 2013-12-04 Oxford Nanopore Tech Ltd Method
GB201314695D0 (en) 2013-08-16 2013-10-02 Oxford Nanopore Tech Ltd Method
EP2970951B1 (en) 2013-03-13 2019-02-20 Illumina, Inc. Methods for nucleic acid sequencing
US9567632B2 (en) 2013-03-19 2017-02-14 New England Biolabs, Inc. Enrichment of target sequences
EP3038738B1 (en) 2013-08-30 2019-02-27 University of Washington through its Center for Commercialization Selective modification of polymer subunits to improve nanopore-based analysis
GB201406151D0 (en) 2014-04-04 2014-05-21 Oxford Nanopore Tech Ltd Method
EP3575410A3 (en) 2013-10-18 2020-03-04 Oxford Nanopore Technologies Limited Modified enzymes
GB201321123D0 (en) 2013-11-29 2014-01-15 Linea Ab Q Amplification of circular molecules
EP3097210B2 (en) 2014-01-22 2022-04-20 Oxford Nanopore Technologies Limited Method for attaching one or more polynucleotide binding proteins to a target polynucleotide
US20170226503A1 (en) 2014-02-06 2017-08-10 MetaMixis Biologics, Inc. Methods for Sequential Screening with Co-Culture Based Detection of Metagenomic Elements Conferring Heterologous Metabolite Secretion
GB201403096D0 (en) 2014-02-21 2014-04-09 Oxford Nanopore Tech Ltd Sample preparation method
US10131944B2 (en) 2014-03-24 2018-11-20 The Regents Of The University Of California Molecular adapter for capture and manipulation of transfer RNA
GB201417712D0 (en) 2014-10-07 2014-11-19 Oxford Nanopore Tech Ltd Method
SG11201610357UA (en) 2014-06-13 2017-01-27 Illumina Cambridge Ltd Methods and compositions for preparing sequencing libraries
US10017759B2 (en) 2014-06-26 2018-07-10 Illumina, Inc. Library preparation of tagged nucleic acid
WO2016003814A1 (en) 2014-06-30 2016-01-07 Illumina, Inc. Methods and compositions using one-sided transposition
WO2016022557A1 (en) 2014-08-05 2016-02-11 Twist Bioscience Corporation Cell free cloning of nucleic acids
US10435685B2 (en) 2014-08-19 2019-10-08 Pacific Biosciences Of California, Inc. Compositions and methods for enrichment of nucleic acids
EP3183367B1 (en) 2014-08-19 2019-06-26 Pacific Biosciences Of California, Inc. Compositions and methods for enrichment of nucleic acids
CN119899249A (zh) 2014-09-01 2025-04-29 弗拉芒区生物技术研究所 突变csgg孔
GB201418159D0 (en) 2014-10-14 2014-11-26 Oxford Nanopore Tech Ltd Method
US10557134B2 (en) 2015-02-24 2020-02-11 Trustees Of Boston University Protection of barcodes during DNA amplification using molecular hairpins
JP6560336B2 (ja) 2015-02-26 2019-08-14 株式会社日立ハイテクノロジーズ 核酸分子の構築方法
GB201609220D0 (en) 2016-05-25 2016-07-06 Oxford Nanopore Tech Ltd Method
CN107488656B (zh) 2016-06-13 2020-07-17 陆欣华 一种核酸等温自扩增方法
GB201807793D0 (en) 2018-05-14 2018-06-27 Oxford Nanopore Tech Ltd Method

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