CN101186940B - 获得具有正确键合的胱氨酸键的胰岛素或胰岛素衍生物的方法 - Google Patents

获得具有正确键合的胱氨酸键的胰岛素或胰岛素衍生物的方法 Download PDF

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Publication number
CN101186940B
CN101186940B CN2006101000625A CN200610100062A CN101186940B CN 101186940 B CN101186940 B CN 101186940B CN 2006101000625 A CN2006101000625 A CN 2006101000625A CN 200610100062 A CN200610100062 A CN 200610100062A CN 101186940 B CN101186940 B CN 101186940B
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China
Prior art keywords
insulin
desired method
halfcystine
concentration
liquid
Prior art date
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Expired - Lifetime
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CN2006101000625A
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English (en)
Chinese (zh)
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CN101186940A (zh
Inventor
F-J·鲁伯洛德
R·凯勒
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Sanofi Aventis Deutschland GmbH
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Aventis Pharma Deutschland GmbH
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Publication of CN101186940A publication Critical patent/CN101186940A/zh
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/12—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by hydrolysis, i.e. solvolysis in general
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
    • A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P5/00—Drugs for disorders of the endocrine system
    • A61P5/48—Drugs for disorders of the endocrine system of the pancreatic hormones
    • A61P5/50—Drugs for disorders of the endocrine system of the pancreatic hormones for increasing or potentiating the activity of insulin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/02—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length in solution
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Diabetes (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Endocrinology (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Veterinary Medicine (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • Engineering & Computer Science (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Hematology (AREA)
  • Obesity (AREA)
  • Emergency Medicine (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN2006101000625A 1997-08-18 1998-08-17 获得具有正确键合的胱氨酸键的胰岛素或胰岛素衍生物的方法 Expired - Lifetime CN101186940B (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19735711A DE19735711C2 (de) 1997-08-18 1997-08-18 Verfahren zur Herstellung eines Vorläufers von Insulin oder Insulinderivaten mit korrekt verbundenen Cystinbrücken
DE19735711.3 1997-08-18

Related Parent Applications (2)

Application Number Title Priority Date Filing Date
CN98117909A Division CN1132845C (zh) 1997-08-18 1998-08-17 获得具有正确键合的胱氨酸键的胰岛素前体的改进的方法
CN031523307A Division CN1483831B (zh) 1997-08-18 1998-08-17 获得具有正确键合的胱氨酸键的胰岛素或胰岛素衍生物的方法

Publications (2)

Publication Number Publication Date
CN101186940A CN101186940A (zh) 2008-05-28
CN101186940B true CN101186940B (zh) 2012-02-08

Family

ID=7839275

Family Applications (3)

Application Number Title Priority Date Filing Date
CN2006101000625A Expired - Lifetime CN101186940B (zh) 1997-08-18 1998-08-17 获得具有正确键合的胱氨酸键的胰岛素或胰岛素衍生物的方法
CN98117909A Expired - Lifetime CN1132845C (zh) 1997-08-18 1998-08-17 获得具有正确键合的胱氨酸键的胰岛素前体的改进的方法
CN031523307A Expired - Lifetime CN1483831B (zh) 1997-08-18 1998-08-17 获得具有正确键合的胱氨酸键的胰岛素或胰岛素衍生物的方法

Family Applications After (2)

Application Number Title Priority Date Filing Date
CN98117909A Expired - Lifetime CN1132845C (zh) 1997-08-18 1998-08-17 获得具有正确键合的胱氨酸键的胰岛素前体的改进的方法
CN031523307A Expired - Lifetime CN1483831B (zh) 1997-08-18 1998-08-17 获得具有正确键合的胱氨酸键的胰岛素或胰岛素衍生物的方法

Country Status (20)

Country Link
US (3) US5986048A (enExample)
EP (2) EP0980874B1 (enExample)
JP (1) JP4291900B2 (enExample)
KR (2) KR100537842B1 (enExample)
CN (3) CN101186940B (enExample)
AR (1) AR016821A1 (enExample)
AT (2) ATE217636T1 (enExample)
AU (1) AU744824B2 (enExample)
BR (2) BR9803755A (enExample)
CA (1) CA2245151C (enExample)
CZ (1) CZ295679B6 (enExample)
DE (3) DE19735711C2 (enExample)
DK (2) DK0980874T3 (enExample)
ES (2) ES2176870T3 (enExample)
HU (2) HU228161B1 (enExample)
ID (1) ID20717A (enExample)
PL (1) PL191901B1 (enExample)
PT (2) PT980874E (enExample)
RU (2) RU2205836C2 (enExample)
TR (1) TR199801587A3 (enExample)

Families Citing this family (34)

* Cited by examiner, † Cited by third party
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DE19735711C2 (de) 1997-08-18 2001-04-26 Aventis Pharma Gmbh Verfahren zur Herstellung eines Vorläufers von Insulin oder Insulinderivaten mit korrekt verbundenen Cystinbrücken
DE19930676B4 (de) 1999-07-02 2006-01-19 Sanofi-Aventis Deutschland Gmbh Verfahren zur Stabilisierung von Insulin, Insulinderivaten und/oder deren Vorläufer in komplexen Mischungen bei deren Lagerung in wäßrigen Lösungsmitteln
JP2003530316A (ja) 1999-12-22 2003-10-14 ノボ ノルディスク アクティーゼルスカブ スクランブルした一本鎖ポリペプチドの抽出的再生法
AU2001280436A1 (en) * 2000-07-12 2002-01-21 Eli Lilly And Company Process to increase protein stability
DE10235168A1 (de) * 2002-08-01 2004-02-12 Aventis Pharma Deutschland Gmbh Verfahren zur Reinigung von Preproinsulin
DE102004015965A1 (de) * 2004-04-01 2005-10-20 Aventis Pharma Gmbh Verfahren zur Gewinnung von Insulinen durch verbesserte Luftbegasung der Faltung
JP5036727B2 (ja) 2005-12-22 2012-09-26 ジェネンテック， インコーポレイテッド ヘパリン結合タンパク質の組み換え体産生
CN101506222B (zh) * 2006-07-14 2013-10-02 健泰科生物技术公司 重组蛋白的重折叠
CA2692585A1 (en) * 2007-07-03 2010-01-04 Amgen Inc. Measurement of protein using inclusion body dry weight
RU2451750C2 (ru) * 2007-09-24 2012-05-27 Общество С Ограниченной Ответственностью "Герофарм" Способ получения рекомбинантного с-пептида проинсулина человека
BRPI0907371A2 (pt) 2008-01-09 2015-11-24 Sanofi Aventis Deutschland derivados de insulina com um perfil de tempo-ação muito retardado
US9296806B2 (en) * 2008-04-30 2016-03-29 Wockhardt Limited Processes for refolding of insulin
RS59913B1 (sr) 2008-10-17 2020-03-31 Sanofi Aventis Deutschland Kombinacija insulina i glp-1-agonista
CA2780043C (en) 2009-11-13 2019-01-29 Sanofi-Aventis Deutschland Gmbh Pharmaceutical composition comprising a glp-1 agonist and methionine
ES2534191T3 (es) 2009-11-13 2015-04-20 Sanofi-Aventis Deutschland Gmbh Composición farmacéutica que comprende un agonista de GLP-1, una insulina y metionina
SG187904A1 (en) 2010-08-30 2013-04-30 Sanofi Aventis Deutschland Use of ave0010 for the manufacture of a medicament for the treatment of diabetes mellitus type 2
US9821032B2 (en) 2011-05-13 2017-11-21 Sanofi-Aventis Deutschland Gmbh Pharmaceutical combination for improving glycemic control as add-on therapy to basal insulin
AR087744A1 (es) 2011-09-01 2014-04-16 Sanofi Aventis Deutschland Composicion farmaceutica para uso en el tratamiento de una enfermedad neurodegenerativa
US12157766B2 (en) * 2012-08-05 2024-12-03 Absci Corporation Cytoplasmic expression system
US10421795B2 (en) 2012-12-17 2019-09-24 Merck Sharp & Dohme Corp. Process for purifying insulin and analogues thereof
WO2014133324A1 (ko) * 2013-02-26 2014-09-04 한미약품 주식회사 신규한 인슐린 아날로그 및 이의 용도
CN103694339B (zh) * 2013-12-04 2017-10-13 珠海联邦制药股份有限公司 一种甘精胰岛素前体的复性方法
US10407483B2 (en) 2014-03-14 2019-09-10 Merck Sharp & Dohme Corp. Purifying insulin using cation exchange and reverse phase chromatography in the presence of an organic modifier and elevated temperature
SI3229828T1 (sl) 2014-12-12 2023-06-30 Sanofi-Aventis Deutschland Gmbh Formulacija s fiksnim razmerjem inzulin glargin/liksisenatid
TWI748945B (zh) 2015-03-13 2021-12-11 德商賽諾菲阿凡提斯德意志有限公司 第２型糖尿病病患治療
TW201705975A (zh) 2015-03-18 2017-02-16 賽諾菲阿凡提斯德意志有限公司 第２型糖尿病病患之治療
CN104911203A (zh) * 2015-06-30 2015-09-16 成都易胜科生物科技有限公司 一种重组人胰岛素的工业制备方法
EP3344651B1 (en) 2015-09-02 2022-03-02 Merck Sharp & Dohme Corp. A process for obtaining insulin with correctly formed disulfide bonds
US10689430B2 (en) 2016-05-25 2020-06-23 Merck Sharp & Dohme Corp. Insulin receptor partial agonists
WO2018018613A1 (zh) 2016-07-29 2018-02-01 广东东阳光药业有限公司 一种提高抗体纯度的细胞培养基和培养方法
CN113773400B (zh) * 2020-06-09 2023-08-18 宁波鲲鹏生物科技有限公司 一种门冬胰岛素衍生物及其应用
CN113773391B (zh) * 2020-06-09 2023-10-20 宁波鲲鹏生物科技有限公司 一种门冬胰岛素的制备方法
CN113773396A (zh) * 2020-06-10 2021-12-10 宁波鲲鹏生物科技有限公司 一种地特胰岛素衍生物及其应用
JP7749397B2 (ja) * 2021-09-30 2025-10-06 キヤノン株式会社 超音波ファントム及び超音波ファントムの製造方法

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0600372A1 (de) * 1992-12-02 1994-06-08 Hoechst Aktiengesellschaft Verfahren zur Gewinnung von Proinsulin mit korrekt verbundenen Cystinbrücken
EP0668292A2 (de) * 1994-02-18 1995-08-23 Hoechst Aktiengesellschaft Verfahren zur Gewinnung von Insulin mit korrekt verbundenen Cystinbrücken
WO1996020724A1 (en) * 1994-12-29 1996-07-11 Bio-Technology General Corp. Generation of human insulin

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NZ199391A (en) * 1981-01-02 1985-12-13 Genentech Inc Chimeric polypeptides comprising a proinsulin sequence,and preparation by recombinant dna technique;production of human insulin
DE3501641A1 (de) * 1985-01-19 1986-07-24 Hoechst Ag, 6230 Frankfurt Verfahren zur gewinnung von insulin-vorlaeufern aus reaktionsgemischen, die bei der faltung von insulin-vorlaeufern aus den entsprechenden s-sulfonaten anfallen
GR1005153B (el) * 1989-08-29 2006-03-13 The General Hospital Corporation Πρωτεινες συντηξεως, παρασκευη τους και χρηση τους.
RU2045535C1 (ru) * 1991-06-18 1995-10-10 Институт биоорганической химии им.М.М.Шемякина и Ю.А.Овчинникова РАН Способ получения проинсулина человека
RU2081122C1 (ru) * 1994-06-16 1997-06-10 Институт биоорганической химии им.М.М.Шемякина и Ю.А.Овчинникова РАН Способ выделения и очистки инсулина или его биотехнологических предшественников
DE19735711C2 (de) 1997-08-18 2001-04-26 Aventis Pharma Gmbh Verfahren zur Herstellung eines Vorläufers von Insulin oder Insulinderivaten mit korrekt verbundenen Cystinbrücken

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0600372A1 (de) * 1992-12-02 1994-06-08 Hoechst Aktiengesellschaft Verfahren zur Gewinnung von Proinsulin mit korrekt verbundenen Cystinbrücken
EP0668292A2 (de) * 1994-02-18 1995-08-23 Hoechst Aktiengesellschaft Verfahren zur Gewinnung von Insulin mit korrekt verbundenen Cystinbrücken
WO1996020724A1 (en) * 1994-12-29 1996-07-11 Bio-Technology General Corp. Generation of human insulin

Also Published As

Publication number Publication date
EP0980874B1 (de) 2002-05-02
TR199801587A2 (xx) 1999-03-22
HU228161B1 (en) 2013-01-28
CN1209437A (zh) 1999-03-03
HU9801886D0 (en) 1998-10-28
DK0980874T3 (da) 2002-08-19
HUP9801886A3 (en) 1999-06-28
CA2245151C (en) 2009-10-06
EP0906918B1 (de) 2002-05-15
JPH11130798A (ja) 1999-05-18
ATE217012T1 (de) 2002-05-15
ES2177178T3 (es) 2002-12-01
CN1483831B (zh) 2012-06-13
HK1119449A1 (en) 2009-03-06
DE19735711A1 (de) 1999-02-25
HK1061870A1 (en) 2004-10-08
CN1483831A (zh) 2004-03-24
AU744824B2 (en) 2002-03-07
DE59804121D1 (de) 2002-06-20
ATE217636T1 (de) 2002-06-15
KR100574580B1 (ko) 2006-04-28
PL328108A1 (en) 1999-03-01
JP4291900B2 (ja) 2009-07-08
PT906918E (pt) 2002-10-31
US6727346B2 (en) 2004-04-27
CZ259898A3 (cs) 1999-03-17
HK1018464A1 (en) 1999-12-24
CN101186940A (zh) 2008-05-28
KR100537842B1 (ko) 2006-03-23
BRPI9816233B8 (pt) 2021-05-25
HUP9801886A2 (hu) 1999-01-28
CA2245151A1 (en) 1999-02-18
HU228203B1 (en) 2013-01-28
CN1132845C (zh) 2003-12-31
DE19735711C2 (de) 2001-04-26
RU2205836C2 (ru) 2003-06-10
TR199801587A3 (tr) 1999-03-22
HU0600287D0 (en) 2006-05-29
US20020156234A1 (en) 2002-10-24
CZ295679B6 (cs) 2005-09-14
KR19990023651A (ko) 1999-03-25
AU8076398A (en) 1999-02-25
US6380355B1 (en) 2002-04-30
EP0906918A2 (de) 1999-04-07
BR9803755A (pt) 2000-03-28
ES2176870T3 (es) 2002-12-01
KR20050067371A (ko) 2005-07-01
PT980874E (pt) 2002-10-31
RU2302882C2 (ru) 2007-07-20
US5986048A (en) 1999-11-16
EP0980874A1 (de) 2000-02-23
ID20717A (id) 1999-02-18
DE59803985D1 (de) 2002-06-06
BRPI9816233B1 (pt) 2016-09-27
DK0906918T3 (da) 2002-09-09
AR016821A1 (es) 2001-08-01
EP0906918A3 (de) 1999-08-11
PL191901B1 (pl) 2006-07-31

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