CN100529068C - 用于提高泛酸产量的微生物和方法 - Google Patents

用于提高泛酸产量的微生物和方法 Download PDF

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Publication number
CN100529068C
CN100529068C CNB028038576A CN02803857A CN100529068C CN 100529068 C CN100529068 C CN 100529068C CN B028038576 A CNB028038576 A CN B028038576A CN 02803857 A CN02803857 A CN 02803857A CN 100529068 C CN100529068 C CN 100529068C
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China
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microorganism
gene
pantothenic acid
acid
pantothenate
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Expired - Fee Related
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CNB028038576A
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Chinese (zh)
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CN1571837A (zh
Inventor
R·R·约卡姆
T·A·帕特森
J·G·佩罗
T·赫尔曼
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BASF SE
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BASF SE
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1003—Transferases (2.) transferring one-carbon groups (2.1)
    • C12N9/1014—Hydroxymethyl-, formyl-transferases (2.1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1022—Transferases (2.) transferring aldehyde or ketonic groups (2.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/02—Amides, e.g. chloramphenicol or polyamides; Imides or polyimides; Urethanes, i.e. compounds comprising N-C=O structural element or polyurethanes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y201/00—Transferases transferring one-carbon groups (2.1)
    • C12Y201/02—Hydroxymethyl-, formyl- and related transferases (2.1.2)
    • C12Y201/02011—3-Methyl-2-oxobutanoate hydroxymethyltransferase (2.1.2.11), i.e. ketopantoate hydroxymethyltransferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y401/00—Carbon-carbon lyases (4.1)
    • C12Y401/01—Carboxy-lyases (4.1.1)
    • C12Y401/01011—Aspartate 1-decarboxylase (4.1.1.11)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y603/00—Ligases forming carbon-nitrogen bonds (6.3)
    • C12Y603/02—Acid—amino-acid ligases (peptide synthases)(6.3.2)
    • C12Y603/02001—Pantoate-beta-alanine ligase (6.3.2.1)

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Fodder In General (AREA)
CNB028038576A 2001-01-19 2002-01-18 用于提高泛酸产量的微生物和方法 Expired - Fee Related CN100529068C (zh)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US26305301P 2001-01-19 2001-01-19
US26299501P 2001-01-19 2001-01-19
US60/263,053 2001-01-19
US60/262,995 2001-01-19
US34763802P 2002-01-11 2002-01-11
US60/347,638 2002-01-11

Publications (2)

Publication Number Publication Date
CN1571837A CN1571837A (zh) 2005-01-26
CN100529068C true CN100529068C (zh) 2009-08-19

Family

ID=27401561

Family Applications (1)

Application Number Title Priority Date Filing Date
CNB028038576A Expired - Fee Related CN100529068C (zh) 2001-01-19 2002-01-18 用于提高泛酸产量的微生物和方法

Country Status (17)

Country Link
US (2) US7244593B2 (enExample)
EP (1) EP1390519B1 (enExample)
JP (1) JP4229700B2 (enExample)
KR (1) KR20030075163A (enExample)
CN (1) CN100529068C (enExample)
AT (1) ATE347589T1 (enExample)
BR (1) BR0206586A (enExample)
CA (1) CA2434626A1 (enExample)
CZ (1) CZ20031886A3 (enExample)
DE (1) DE60216581T2 (enExample)
HU (1) HU227432B1 (enExample)
IL (1) IL156708A0 (enExample)
IN (2) IN2003CH01278A (enExample)
MX (1) MXPA03006343A (enExample)
NO (1) NO20033240L (enExample)
PL (1) PL369009A1 (enExample)
WO (1) WO2002061108A2 (enExample)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8232081B2 (en) * 1999-09-21 2012-07-31 Basf Se Methods and microorganisms for production of panto-compounds
BR0206587A (pt) * 2001-01-19 2005-12-13 Basf Ag Processo para a produção de uma composição de pantotenato livre de hmbpa, produto, composição de pantotenato livre de hmbpa, composição sintetizada por um microorganismo possuindo uma rota biossintética de pantotenato desregulada, microorganismo recombinante para a produção de composições de pantotenato livres de hmbpa, e, processo para a produção de uma composição de pantotenato: hmbpa seletivamente misturada
DE10106461A1 (de) * 2001-02-13 2002-08-14 Degussa Verfahren zur fermentativen Herstellung von D-Pantothensäure und/oder deren Salzen
DE10108223A1 (de) 2001-02-21 2002-10-10 Basf Ag Verfahren zur Herstellung von D-Pantothensäure und/oder deren Salze als Zusatz zu Tierfuttermitteln
CN1294271C (zh) 2001-02-21 2007-01-10 巴斯福股份公司 制备作为动物饲料添加剂的d－泛酸和/或其盐的方法
EP1247868A3 (de) * 2001-04-03 2002-10-30 Degussa AG Verfahren zur fermentativen Herstellung von D-Pantothensäure und/oder deren Salzen
DE10132178A1 (de) 2001-07-03 2003-01-23 Degussa Verfahren zur fermantativen Herstellung von D-Pantothensäure und/oder deren Salzen
EP1402046A1 (en) * 2001-07-03 2004-03-31 Degussa AG Process for the fermentative preparation of d-pantothenic acid and/or salts thereof
US20060099692A1 (en) * 2002-07-03 2006-05-11 Yocum R R Microorganisms and processes for enhanced production of pantothenate
MXPA04012812A (es) * 2002-07-03 2005-02-24 Basf Ag Microorganismos y proceso para la produccion aumentada de pantotenato.
KR101185656B1 (ko) * 2003-06-18 2012-09-24 디에스엠 아이피 어셋츠 비.브이. 포자를 형성할 수 없는 미생물을 사용한 판토텐산염의 제조방법
DE10331291A1 (de) 2003-07-10 2005-02-17 Consortium für elektrochemische Industrie GmbH Varianten der 3-Phosphoglyceratdehydrogenase mit reduzierter Hemmung durch L-Serin und dafür codierende Gene
DE102004026152A1 (de) * 2004-05-28 2005-12-15 Basf Ag Fermentative Herstellung von Feinchemikalien
CN101448950B (zh) 2006-05-16 2014-02-12 帝斯曼知识产权资产管理有限公司 用于生产泛醇的工艺
EP2157174A1 (en) 2008-08-12 2010-02-24 DSM IP Assets B.V. Increased production of pantothenate (vitamin B5)
US20110117087A1 (en) 2009-10-26 2011-05-19 Reinhard Franze Method for the production of a glycosylated immunoglobulin
CN102311966B (zh) * 2010-06-30 2016-04-13 中国科学院青岛生物能源与过程研究所 用于合成脂肪醇的构建体，载体，蓝细菌，以及在蓝细菌中生产脂肪醇的方法
EP2723878B1 (en) 2011-06-21 2020-05-20 DSM IP Assets B.V. Vitamin b5 production
WO2012175574A1 (en) 2011-06-21 2012-12-27 Dsm Ip Assets B.V. Increased pantothenate (vitamin b5) production
CA2862051A1 (en) 2012-01-30 2013-08-08 Myriant Corporation Production of muconic acid from genetically engineered microorganisms
WO2014087184A1 (en) * 2012-12-03 2014-06-12 Metabolic Explorer Neopentyl glycol fermentative production by a recombinant microorganism
WO2017151811A1 (en) 2016-03-02 2017-09-08 Myriant Corporation Improved muconic acid production from genetically engineered microorganisms
CN108456701B (zh) * 2018-03-23 2020-10-16 精晶药业股份有限公司 一种d-泛解酸内酯的制备方法
CN111534562A (zh) * 2020-06-19 2020-08-14 南京欧信医药技术有限公司 一种d-泛解酸的制备方法
CN112195143B (zh) * 2020-09-24 2022-10-14 浙江工业大学 一种用于发酵法生产d-泛酸的菌株及发酵法生产d-泛酸的方法
CN113416744B (zh) * 2021-06-04 2022-05-20 浙江工业大学 一种d-泛解酸生产菌、构建方法及应用
CN116024278B (zh) * 2022-12-16 2024-05-07 黑龙江新和成生物科技有限公司 一种发酵法制备d-泛酸的方法
WO2025113965A1 (en) * 2023-11-29 2025-06-05 Unilever Ip Holdings B.V. Use of l-aspartate for improving production of pantothenate

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1254758A (zh) * 1998-10-09 2000-05-31 底古萨－胡尔斯股份公司 通过扩增编码酮泛解酸盐还原酶的核苷酸序列制备泛酸的方法
CN1496405A (zh) * 2001-01-19 2004-05-12 �����ɷ� 提高泛酸产量的方法

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6787334B1 (en) * 1998-10-09 2004-09-07 Degussa Ag Process for the preparation of pantothenic acid by amplification of nucleotide sequences which code for the ketopantoate reductase
DE19855314A1 (de) * 1998-12-01 2000-06-08 Degussa Verfahren zur fermentiven Herstellung von D-Pantothensäure unter Verwendung von Stämmen der Familie Enterobacteriaceae
DE19855312A1 (de) * 1998-12-01 2000-06-08 Degussa Verfahren zur fermentativen Herstellung von D-Pantothensäure unter Verwendung coryneformer Bakterien
DE19855313A1 (de) * 1998-12-01 2000-06-08 Degussa Verfahren zur fermentativen Herstellung von D-Pantothensäure durch Verstärkung des panD-Gens in Mikroorganismen
MXPA02003017A (es) * 1999-09-21 2004-09-06 Basf Ag Metodos y microorganismos para la produccion de panto-compuestos.
EP1247868A3 (de) * 2001-04-03 2002-10-30 Degussa AG Verfahren zur fermentativen Herstellung von D-Pantothensäure und/oder deren Salzen
EP1402046A1 (en) * 2001-07-03 2004-03-31 Degussa AG Process for the fermentative preparation of d-pantothenic acid and/or salts thereof

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1254758A (zh) * 1998-10-09 2000-05-31 底古萨－胡尔斯股份公司 通过扩增编码酮泛解酸盐还原酶的核苷酸序列制备泛酸的方法
CN1496405A (zh) * 2001-01-19 2004-05-12 �����ɷ� 提高泛酸产量的方法

Also Published As

Publication number Publication date
EP1390519B1 (en) 2006-12-06
NO20033240D0 (no) 2003-07-17
NO20033240L (no) 2003-09-17
DE60216581D1 (de) 2007-01-18
IL156708A0 (en) 2004-01-04
JP2004533213A (ja) 2004-11-04
AU2002243526A (en) 2002-08-12
IN2003CH01280A (enExample) 2005-11-18
HUP0501185A2 (en) 2006-10-28
CZ20031886A3 (cs) 2003-10-15
CN1571837A (zh) 2005-01-26
BR0206586A (pt) 2006-01-24
MXPA03006343A (es) 2003-10-06
IN2003CH01278A (enExample) 2005-11-18
WO2002061108A2 (en) 2002-08-08
ATE347589T1 (de) 2006-12-15
DE60216581T2 (de) 2007-09-27
CA2434626A1 (en) 2002-08-08
PL369009A1 (en) 2005-04-18
WO2002061108A3 (en) 2003-12-11
US20040091979A1 (en) 2004-05-13
HU227432B1 (hu) 2011-06-28
EP1390519A2 (en) 2004-02-25
US7244593B2 (en) 2007-07-17
JP4229700B2 (ja) 2009-02-25
US20080166777A1 (en) 2008-07-10
KR20030075163A (ko) 2003-09-22

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