CH615460A5 - - Google Patents
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- Publication number
- CH615460A5 CH615460A5 CH1267775A CH1267775A CH615460A5 CH 615460 A5 CH615460 A5 CH 615460A5 CH 1267775 A CH1267775 A CH 1267775A CH 1267775 A CH1267775 A CH 1267775A CH 615460 A5 CH615460 A5 CH 615460A5
- Authority
- CH
- Switzerland
- Prior art keywords
- zone
- buffer
- solution
- substrate
- absorbent material
- Prior art date
Links
- 239000000243 solution Substances 0.000 claims description 57
- 239000000758 substrate Substances 0.000 claims description 54
- 239000002250 absorbent Substances 0.000 claims description 53
- 230000002745 absorbent Effects 0.000 claims description 53
- 239000000872 buffer Substances 0.000 claims description 34
- 239000000463 material Substances 0.000 claims description 30
- KUWPCJHYPSUOFW-YBXAARCKSA-N 2-nitrophenyl beta-D-galactoside Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1OC1=CC=CC=C1[N+]([O-])=O KUWPCJHYPSUOFW-YBXAARCKSA-N 0.000 claims description 28
- 229940039227 diagnostic agent Drugs 0.000 claims description 27
- 239000000032 diagnostic agent Substances 0.000 claims description 27
- LWIHDJKSTIGBAC-UHFFFAOYSA-K tripotassium phosphate Chemical compound [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 claims description 20
- 239000007853 buffer solution Substances 0.000 claims description 19
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims description 16
- 102000005936 beta-Galactosidase Human genes 0.000 claims description 16
- 108010005774 beta-Galactosidase Proteins 0.000 claims description 16
- 239000003153 chemical reaction reagent Substances 0.000 claims description 16
- 238000000926 separation method Methods 0.000 claims description 15
- 238000000034 method Methods 0.000 claims description 13
- 239000000725 suspension Substances 0.000 claims description 11
- 229910000160 potassium phosphate Inorganic materials 0.000 claims description 10
- 235000011009 potassium phosphates Nutrition 0.000 claims description 10
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 claims description 9
- 244000005700 microbiome Species 0.000 claims description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 9
- 238000002360 preparation method Methods 0.000 claims description 8
- 238000004519 manufacturing process Methods 0.000 claims description 7
- 239000007788 liquid Substances 0.000 claims description 6
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 5
- 238000001514 detection method Methods 0.000 claims description 5
- 230000007062 hydrolysis Effects 0.000 claims description 4
- 238000006460 hydrolysis reaction Methods 0.000 claims description 4
- 230000001580 bacterial effect Effects 0.000 claims description 3
- 230000004069 differentiation Effects 0.000 claims description 3
- 239000011780 sodium chloride Substances 0.000 claims description 3
- -1 o -Nitrophenyl- Chemical group 0.000 claims 1
- 239000012876 carrier material Substances 0.000 description 28
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 15
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 13
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 7
- YXFVVABEGXRONW-UHFFFAOYSA-N Toluene Chemical compound CC1=CC=CC=C1 YXFVVABEGXRONW-UHFFFAOYSA-N 0.000 description 6
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 6
- 238000001035 drying Methods 0.000 description 5
- 238000011534 incubation Methods 0.000 description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Natural products CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- 239000012153 distilled water Substances 0.000 description 4
- 238000003756 stirring Methods 0.000 description 4
- 239000013078 crystal Substances 0.000 description 3
- 238000002405 diagnostic procedure Methods 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- RGHFKWPGWBFQLN-UHFFFAOYSA-M sodium;5,5-diethylpyrimidin-3-ide-2,4,6-trione Chemical compound [Na+].CCC1(CC)C([O-])=NC(=O)NC1=O RGHFKWPGWBFQLN-UHFFFAOYSA-M 0.000 description 3
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- 239000011248 coating agent Substances 0.000 description 2
- 238000000576 coating method Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- YMAWOPBAYDPSLA-UHFFFAOYSA-N glycylglycine Chemical compound [NH3+]CC(=O)NCC([O-])=O YMAWOPBAYDPSLA-UHFFFAOYSA-N 0.000 description 2
- 230000002209 hydrophobic effect Effects 0.000 description 2
- BDERNNFJNOPAEC-UHFFFAOYSA-N propan-1-ol Chemical compound CCCO BDERNNFJNOPAEC-UHFFFAOYSA-N 0.000 description 2
- 239000012064 sodium phosphate buffer Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 2
- WQZGKKKJIJFFOK-SVZMEOIVSA-N (+)-Galactose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-SVZMEOIVSA-N 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 241000589513 Burkholderia cepacia Species 0.000 description 1
- VYZAMTAEIAYCRO-UHFFFAOYSA-N Chromium Chemical compound [Cr] VYZAMTAEIAYCRO-UHFFFAOYSA-N 0.000 description 1
- 241000588923 Citrobacter Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 108010008488 Glycylglycine Proteins 0.000 description 1
- VVQNEPGJFQJSBK-UHFFFAOYSA-N Methyl methacrylate Chemical compound COC(=O)C(C)=C VVQNEPGJFQJSBK-UHFFFAOYSA-N 0.000 description 1
- BAVYZALUXZFZLV-UHFFFAOYSA-N Methylamine Chemical compound NC BAVYZALUXZFZLV-UHFFFAOYSA-N 0.000 description 1
- GLUUGHFHXGJENI-UHFFFAOYSA-N Piperazine Chemical compound C1CNCCN1 GLUUGHFHXGJENI-UHFFFAOYSA-N 0.000 description 1
- OFOBLEOULBTSOW-UHFFFAOYSA-N Propanedioic acid Natural products OC(=O)CC(O)=O OFOBLEOULBTSOW-UHFFFAOYSA-N 0.000 description 1
- 241000607142 Salmonella Species 0.000 description 1
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
- 241000122973 Stenotrophomonas maltophilia Species 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 238000010876 biochemical test Methods 0.000 description 1
- 229910021538 borax Inorganic materials 0.000 description 1
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 description 1
- 235000010338 boric acid Nutrition 0.000 description 1
- 229960002645 boric acid Drugs 0.000 description 1
- 239000000919 ceramic Substances 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 229910052804 chromium Inorganic materials 0.000 description 1
- 239000011651 chromium Substances 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 238000012937 correction Methods 0.000 description 1
- 239000012502 diagnostic product Substances 0.000 description 1
- 239000003085 diluting agent Substances 0.000 description 1
- 125000001495 ethyl group Chemical group [H]C([H])([H])C([H])([H])* 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 229940043257 glycylglycine Drugs 0.000 description 1
- CVVIJWRCGSYCMB-UHFFFAOYSA-N hydron;piperazine;dichloride Chemical compound Cl.Cl.C1CNCCN1 CVVIJWRCGSYCMB-UHFFFAOYSA-N 0.000 description 1
- 125000004029 hydroxymethyl group Chemical group [H]OC([H])([H])* 0.000 description 1
- 125000001449 isopropyl group Chemical group [H]C([H])([H])C([H])(*)C([H])([H])[H] 0.000 description 1
- 238000003771 laboratory diagnosis Methods 0.000 description 1
- 238000011005 laboratory method Methods 0.000 description 1
- 239000004922 lacquer Substances 0.000 description 1
- VZCYOOQTPOCHFL-UPHRSURJSA-N maleic acid Chemical compound OC(=O)\C=C/C(O)=O VZCYOOQTPOCHFL-UPHRSURJSA-N 0.000 description 1
- 239000011976 maleic acid Substances 0.000 description 1
- FPYJFEHAWHCUMM-UHFFFAOYSA-N maleic anhydride Chemical compound O=C1OC(=O)C=C1 FPYJFEHAWHCUMM-UHFFFAOYSA-N 0.000 description 1
- OKKJLVBELUTLKV-UHFFFAOYSA-N methanol Natural products OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 1
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 1
- 125000001209 o-nitrophenyl group Chemical group [H]C1=C([H])C(*)=C(C([H])=C1[H])[N+]([O-])=O 0.000 description 1
- 230000035515 penetration Effects 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 239000001632 sodium acetate Substances 0.000 description 1
- 235000017281 sodium acetate Nutrition 0.000 description 1
- 239000001488 sodium phosphate Substances 0.000 description 1
- 229910000162 sodium phosphate Inorganic materials 0.000 description 1
- 239000004328 sodium tetraborate Substances 0.000 description 1
- 235000010339 sodium tetraborate Nutrition 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- VZCYOOQTPOCHFL-UHFFFAOYSA-N trans-butenedioic acid Natural products OC(=O)C=CC(O)=O VZCYOOQTPOCHFL-UHFFFAOYSA-N 0.000 description 1
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 1
- 239000001993 wax Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2334/00—O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/914—Hydrolases (3)
- G01N2333/924—Hydrolases (3) acting on glycosyl compounds (3.2)
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/805—Test papers
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US05/510,816 US3957584A (en) | 1974-09-30 | 1974-09-30 | Detection of beta-galactosidase producing micro-organisms |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CH615460A5 true CH615460A5 (en, 2012) | 1980-01-31 |
Family
ID=24032326
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CH1267775A CH615460A5 (en, 2012) | 1974-09-30 | 1975-09-30 |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US3957584A (en, 2012) |
| JP (1) | JPS5147491A (en, 2012) |
| CA (1) | CA1045531A (en, 2012) |
| CH (1) | CH615460A5 (en, 2012) |
| DK (1) | DK142461B (en, 2012) |
| FR (1) | FR2286192A1 (en, 2012) |
| GB (1) | GB1466828A (en, 2012) |
| IT (1) | IT1054910B (en, 2012) |
| NO (1) | NO148380C (en, 2012) |
| SE (1) | SE7510895L (en, 2012) |
Families Citing this family (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4070247A (en) * | 1977-03-30 | 1978-01-24 | Indiana University Foundation | Diagnostic media |
| US4279992A (en) * | 1978-03-13 | 1981-07-21 | Miles Laboratories, Inc. | Specific binding assay employing an enzyme-cleavable substrate as label |
| US4351899A (en) * | 1978-07-19 | 1982-09-28 | Owen Oliver E | Semi-quantitative assay of metabolic acids |
| US4208480A (en) * | 1978-08-23 | 1980-06-17 | American Home Products Corporation | Method, reagents and apparatus for the rapid identification of Neisseria gonorrhoeae and Neisseria meningitidis |
| US4242447A (en) * | 1978-11-29 | 1980-12-30 | Bioresearch | Rapid detection of bacteria |
| EP0018825B1 (en) * | 1979-05-02 | 1985-02-13 | National Research Development Corporation | A process for the identification of bacteria and a kit of reagents for use in this process |
| FR2457323A1 (fr) * | 1979-05-25 | 1980-12-19 | Api Labor | Test permettant la mise en evidence des bacteries du genre salmonella et serratia et leur distinction des genres proteus et providencia |
| US4713328A (en) * | 1981-09-30 | 1987-12-15 | Yolken Robert H | Microbial Enzyme assays |
| CA1219201A (en) * | 1983-03-07 | 1987-03-17 | Albert E. Chu | Microorganism detection test |
| EP0470172B1 (en) * | 1989-04-27 | 1995-06-28 | BioControl Systems, Incorporated | Precipitate test for microorganisms |
| US6699685B1 (en) | 1990-04-20 | 2004-03-02 | Rcr Scientific, Inc. | Method, test media and chromogenic compounds for identifying and differentiating general coliforms and escherichia coli bacteria |
| US5210022A (en) * | 1990-04-20 | 1993-05-11 | Rcr Scientific, Inc. | Method test media and chromogenic compounds for identifying and differentiating general coliforms and Escherichia coli bacteria |
| US5633144A (en) * | 1990-05-03 | 1997-05-27 | University Of Florida Research Foundation, Inc. | Assay pad and method for determination of the presence of total coliforms |
| FR2671100B1 (fr) * | 1990-12-28 | 1993-03-05 | Bio Merieux | Procede d'analyse bacteriologique, et milieu de detection des bacteries genre salmonella. |
| US5464755A (en) * | 1994-04-29 | 1995-11-07 | Biolog, Inc. | Microbiological medium and method of assay |
| FI98379C (fi) * | 1995-03-24 | 1997-06-10 | Orion Yhtymae Oy | Elatusaine ja menetelmä salmonellojen tunnistamiseksi |
| WO1996040861A1 (en) * | 1995-06-07 | 1996-12-19 | Biolog, Inc. | Microbiological media for isolation and identification of enteric pathogens such as e. coli and salmonella |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3359180A (en) * | 1967-04-04 | 1967-12-19 | Warner Lambert Pharmaceutical | Diagnostic preparation for the detection of acetylmethylcarbinol |
| US3649461A (en) * | 1968-08-09 | 1972-03-14 | Warner Lambert Co | Paper strip test for citrate utilization |
| US3645853A (en) * | 1969-06-24 | 1972-02-29 | Warner Lambert Co | Diagnostic composition and method for the detection of nitrate reduction |
| US3785929A (en) * | 1971-11-30 | 1974-01-15 | Warner Lambert Co | Diagnostic composition for the detection of nitrite |
| CH583422A5 (en, 2012) * | 1972-01-25 | 1976-12-31 | Hoffmann La Roche | |
| JPS5247035B2 (en, 2012) * | 1972-11-24 | 1977-11-29 | ||
| US3870601A (en) * | 1973-05-04 | 1975-03-11 | Schering Corp | Novel diagnostic system for differentiation of enterobacteriaceae |
-
1974
- 1974-09-30 US US05/510,816 patent/US3957584A/en not_active Expired - Lifetime
-
1975
- 1975-07-16 NO NO752544A patent/NO148380C/no unknown
- 1975-07-31 JP JP50093648A patent/JPS5147491A/ja active Granted
- 1975-08-29 GB GB3571175A patent/GB1466828A/en not_active Expired
- 1975-09-25 CA CA236,377A patent/CA1045531A/en not_active Expired
- 1975-09-29 DK DK438675AA patent/DK142461B/da not_active IP Right Cessation
- 1975-09-29 SE SE7510895A patent/SE7510895L/xx unknown
- 1975-09-29 IT IT27731/75A patent/IT1054910B/it active
- 1975-09-30 FR FR7529914A patent/FR2286192A1/fr active Granted
- 1975-09-30 CH CH1267775A patent/CH615460A5/de not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| NO148380B (no) | 1983-06-20 |
| DK142461C (en, 2012) | 1981-03-30 |
| NO752544L (en, 2012) | 1976-03-31 |
| CA1045531A (en) | 1979-01-02 |
| DE2531982B2 (de) | 1977-04-21 |
| DK438675A (en, 2012) | 1976-03-31 |
| US3957584A (en) | 1976-05-18 |
| FR2286192A1 (fr) | 1976-04-23 |
| JPS5431396B2 (en, 2012) | 1979-10-06 |
| FR2286192B1 (en, 2012) | 1979-04-06 |
| AU8300975A (en) | 1977-01-20 |
| JPS5147491A (en) | 1976-04-23 |
| DK142461B (da) | 1980-11-03 |
| NO148380C (no) | 1983-09-28 |
| GB1466828A (en) | 1977-03-09 |
| SE7510895L (sv) | 1976-03-31 |
| IT1054910B (it) | 1981-11-30 |
| DE2531982A1 (de) | 1976-04-08 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PL | Patent ceased | ||
| PL | Patent ceased |