CA3225576A1 - Methods, compositions, and kits for assay signal amplification - Google Patents
Methods, compositions, and kits for assay signal amplification Download PDFInfo
- Publication number
- CA3225576A1 CA3225576A1 CA3225576A CA3225576A CA3225576A1 CA 3225576 A1 CA3225576 A1 CA 3225576A1 CA 3225576 A CA3225576 A CA 3225576A CA 3225576 A CA3225576 A CA 3225576A CA 3225576 A1 CA3225576 A1 CA 3225576A1
- Authority
- CA
- Canada
- Prior art keywords
- reagent
- detectable label
- detectable
- signal amplification
- binding
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/66—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light electrically excited, e.g. electroluminescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
- G01N33/533—Production of labelled immunochemicals with fluorescent label
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
- C07F15/00—Compounds containing elements of Groups 8, 9, 10 or 18 of the Periodic Table
- C07F15/0006—Compounds containing elements of Groups 8, 9, 10 or 18 of the Periodic Table compounds of the platinum group
- C07F15/0046—Ruthenium compounds
- C07F15/0053—Ruthenium compounds without a metal-carbon linkage
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/44—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material not provided for elsewhere, e.g. haptens, metals, DNA, RNA, amino acids
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2458/00—Labels used in chemical analysis of biological material
- G01N2458/10—Oligonucleotides as tagging agents for labelling antibodies
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Organic Chemistry (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Cell Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Optics & Photonics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202163215660P | 2021-06-28 | 2021-06-28 | |
| US63/215,660 | 2021-06-28 | ||
| PCT/US2022/035068 WO2023278293A1 (en) | 2021-06-28 | 2022-06-27 | Methods, compositions, and kits for assay signal amplification |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA3225576A1 true CA3225576A1 (en) | 2023-01-05 |
Family
ID=82703171
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA3225576A Pending CA3225576A1 (en) | 2021-06-28 | 2022-06-27 | Methods, compositions, and kits for assay signal amplification |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US20230020070A1 (enExample) |
| EP (1) | EP4363857A1 (enExample) |
| JP (1) | JP2024526217A (enExample) |
| KR (1) | KR20240051109A (enExample) |
| CN (1) | CN117999482A (enExample) |
| AU (1) | AU2022304570A1 (enExample) |
| CA (1) | CA3225576A1 (enExample) |
| IL (1) | IL309517A (enExample) |
| TW (1) | TW202317992A (enExample) |
| WO (1) | WO2023278293A1 (enExample) |
Family Cites Families (31)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4235601A (en) | 1979-01-12 | 1980-11-25 | Thyroid Diagnostics, Inc. | Test device and method for its use |
| US4442204A (en) | 1981-04-10 | 1984-04-10 | Miles Laboratories, Inc. | Homogeneous specific binding assay device and preformed complex method |
| US5310687A (en) | 1984-10-31 | 1994-05-10 | Igen, Inc. | Luminescent metal chelate labels and means for detection |
| US5591581A (en) | 1986-04-30 | 1997-01-07 | Igen, Inc. | Electrochemiluminescent rhenium moieties and methods for their use |
| US6316607B1 (en) | 1986-04-30 | 2001-11-13 | Igen International, Inc. | Electrochemiluminescent assays |
| US5028535A (en) | 1989-01-10 | 1991-07-02 | Biosite Diagnostics, Inc. | Threshold ligand-receptor assay |
| US5786141A (en) | 1994-08-26 | 1998-07-28 | Bard; Allen J. | Electrogenerated chemiluminescence labels for analysis and/or referencing |
| US5679519A (en) * | 1995-05-09 | 1997-10-21 | Oprandy; John J. | Multi-label complex for enhanced sensitivity in electrochemiluminescence assay |
| US6136268A (en) | 1999-08-17 | 2000-10-24 | Orion Diagnostica | Method for luminescence measurements |
| US6808939B2 (en) | 2001-06-29 | 2004-10-26 | Igen International, Inc. | ECL labels having improved non-specific binding properties, methods of using and kits containing the same |
| JP2004534226A (ja) | 2001-06-29 | 2004-11-11 | メソ スケイル テクノロジーズ,エルエルシー | 発光試験測定用のアッセイプレート、リーダシステム及び方法 |
| EP1451348B1 (en) * | 2001-09-10 | 2015-08-12 | Meso Scale Technologies, LLC | Methods, reagents, kits and apparatus for protein function analysis |
| AU2003302263A1 (en) | 2002-12-26 | 2004-07-29 | Meso Scale Technologies, Llc. | Assay cartridges and methods of using the same |
| US7981362B2 (en) | 2003-11-04 | 2011-07-19 | Meso Scale Technologies, Llc | Modular assay plates, reader systems and methods for test measurements |
| WO2005121798A1 (en) | 2004-06-03 | 2005-12-22 | Meso Scale Technologies, Llc | Methods and apparatuses for conducting assays |
| WO2007076023A2 (en) | 2005-12-21 | 2007-07-05 | Meso Scale Technologies, Llc | Assay modules having assay reagents and methods of making and using same |
| US20110022331A1 (en) | 2009-07-27 | 2011-01-27 | Meso Scale Technologies, Llc | Assay Information Management Methods and Devices |
| CA3028780C (en) | 2009-12-07 | 2022-05-31 | Meso Scale Technologies, Llc | Assay cartridges and methods of using the same |
| CN103392181B (zh) | 2010-07-27 | 2018-04-27 | 梅索磅秤技术有限公司 | 消耗品数据管理 |
| EP2661485A4 (en) | 2011-01-06 | 2018-11-21 | Meso Scale Technologies, LLC | Assay cartridges and methods of using the same |
| EP2870391B1 (en) | 2012-07-05 | 2019-03-27 | Meso Scale Technologies, LLC | Assay cartridge valve system |
| CN104822696B (zh) | 2012-08-02 | 2017-09-22 | 霍夫曼-拉罗奇有限公司 | 用于ecl的新型铱基配合物 |
| US10281678B2 (en) | 2013-01-04 | 2019-05-07 | Meso Scale Technologies, Llc | Assay apparatuses, methods and reagants |
| EP4219750A3 (en) | 2013-03-11 | 2023-12-13 | Meso Scale Technologies, LLC. | Improved methods for conducting multiplexed assays |
| KR102257912B1 (ko) * | 2013-03-13 | 2021-05-27 | 메소 스케일 테크놀러지즈, 엘엘시 | 개선된 분석 방법 |
| US10114015B2 (en) * | 2013-03-13 | 2018-10-30 | Meso Scale Technologies, Llc. | Assay methods |
| CN119709943A (zh) * | 2014-05-15 | 2025-03-28 | 中尺度技术有限责任公司 | 改进的测定方法 |
| EP3280520B1 (en) | 2015-04-06 | 2021-11-03 | Meso Scale Technologies, LLC | High throughput system for performing assays using electrochemiluminescence including a consumable shaking apparatus |
| WO2018017156A1 (en) | 2016-07-22 | 2018-01-25 | Meso Scale Technologies, Llc. | Integrated consumable data management system & platform |
| AU2016297652A1 (en) | 2015-07-23 | 2018-02-15 | Meso Scale Technologies, Llc. | Integrated consumable data management system and platform |
| AU2019418498A1 (en) | 2019-01-03 | 2021-07-15 | Meso Scale Technologies, Llc | Compositions and methods for carrying out assay measurements |
-
2022
- 2022-06-27 AU AU2022304570A patent/AU2022304570A1/en active Pending
- 2022-06-27 IL IL309517A patent/IL309517A/en unknown
- 2022-06-27 US US17/849,917 patent/US20230020070A1/en active Pending
- 2022-06-27 WO PCT/US2022/035068 patent/WO2023278293A1/en not_active Ceased
- 2022-06-27 CA CA3225576A patent/CA3225576A1/en active Pending
- 2022-06-27 JP JP2023579630A patent/JP2024526217A/ja active Pending
- 2022-06-27 EP EP22747837.7A patent/EP4363857A1/en active Pending
- 2022-06-27 CN CN202280051025.7A patent/CN117999482A/zh active Pending
- 2022-06-27 KR KR1020247002999A patent/KR20240051109A/ko active Pending
- 2022-06-28 TW TW111124084A patent/TW202317992A/zh unknown
Also Published As
| Publication number | Publication date |
|---|---|
| EP4363857A1 (en) | 2024-05-08 |
| JP2024526217A (ja) | 2024-07-17 |
| TW202317992A (zh) | 2023-05-01 |
| WO2023278293A1 (en) | 2023-01-05 |
| US20230020070A1 (en) | 2023-01-19 |
| KR20240051109A (ko) | 2024-04-19 |
| CN117999482A (zh) | 2024-05-07 |
| AU2022304570A1 (en) | 2024-01-18 |
| IL309517A (en) | 2024-02-01 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP7769743B2 (ja) | シグナル発生型デジタルアッセイにおけるノイズを低減する方法 | |
| CA2647953A1 (en) | Multiplex analyte detection | |
| Stevens et al. | Conjugating immunoassays to mass spectrometry: Solutions to contemporary challenges in clinical diagnostics | |
| US11835517B2 (en) | Paired microparticles-based kits for detecting analytes | |
| CN109477834A (zh) | 目标分子的检测方法及目标分子检测试剂盒 | |
| US9081016B2 (en) | Assay for measurement of antibodies binding to a therapeutic monoclonal antibody | |
| JP2008545145A (ja) | 対応治療抗体の有無を問わない標的抗原の検出 | |
| WO2006128362A1 (fr) | Procede et kit correspondant destines a la detection quantitative d’un analyte specifique avec un agent de capture unique | |
| US20130165335A1 (en) | Multiplex measure of isotype antigen response | |
| WO2022109196A1 (en) | Multiplexed imaging with nanobody probes | |
| US20230020070A1 (en) | Methods, compositions, and kits for assay signal amplification | |
| JPH10511460A (ja) | 被験サンプル中の複数分析物の時差式検出方法 | |
| US8969256B2 (en) | Solution microarrays and uses thereof | |
| CN104995512A (zh) | 用于可溶性成纤维细胞生长因子受体的灵敏的多重免疫测定 | |
| CN110121364A (zh) | 肽核酸缀合物 | |
| US20130310277A1 (en) | Multi-analyte microarrays using tag-specific antibodies and tag-anchored antibodies | |
| US12411129B2 (en) | Reduction of interferences in immunoassays | |
| CA3152467A1 (en) | Multiplex measure of isotype antigen response | |
| US20140274758A1 (en) | Antibody profiling, methods and apparatus for identifying an individual or source of a biological material | |
| RU2519023C2 (ru) | Способ детектирования концентрации аналита с широким динамическим диапазоном | |
| KR101432714B1 (ko) | 벤조안트라센 오염 검출을 위한 마커 및 키트 | |
| WO2024241162A1 (en) | Peptide for site-specific antibody conjugation and method of use | |
| US20030224459A1 (en) | Protein detection method | |
| CN117836627A (zh) | 从脱石蜡细胞开始的单细胞elisa用于检测感兴趣分子的用途 | |
| Normandeau | Slide spatial bias in antibody microarrays: from characterization to minimization |