JP2024526217A - アッセイシグナル増幅のための方法、組成物及びキット - Google Patents

アッセイシグナル増幅のための方法、組成物及びキット Download PDF

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JP2024526217A
JP2024526217A JP2023579630A JP2023579630A JP2024526217A JP 2024526217 A JP2024526217 A JP 2024526217A JP 2023579630 A JP2023579630 A JP 2023579630A JP 2023579630 A JP2023579630 A JP 2023579630A JP 2024526217 A JP2024526217 A JP 2024526217A
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Prior art keywords
reagent
detectable label
detectable
binding
signal amplification
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JPWO2023278293A5 (enExample
Inventor
ケンテン,ジョン
シガール,ジョージ
ケー. タッカー-シュワルツ,アレクサンダー
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メソ スケール テクノロジーズ エルエルシー
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Publication of JPWO2023278293A5 publication Critical patent/JPWO2023278293A5/ja
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/581Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07FACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F15/00Compounds containing elements of Groups 8, 9, 10 or 18 of the Periodic Table
    • C07F15/0006Compounds containing elements of Groups 8, 9, 10 or 18 of the Periodic Table compounds of the platinum group
    • C07F15/0046Ruthenium compounds
    • C07F15/0053Ruthenium compounds without a metal-carbon linkage
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/6428Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/66Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light electrically excited, e.g. electroluminescence
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531Production of immunochemical test materials
    • G01N33/532Production of labelled immunochemicals
    • G01N33/533Production of labelled immunochemicals with fluorescent label
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/44Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material not provided for elsewhere, e.g. haptens, metals, DNA, RNA, amino acids
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/6428Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2458/00Labels used in chemical analysis of biological material
    • G01N2458/10Oligonucleotides as tagging agents for labelling antibodies

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Optics & Photonics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
JP2023579630A 2021-06-28 2022-06-27 アッセイシグナル増幅のための方法、組成物及びキット Pending JP2024526217A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202163215660P 2021-06-28 2021-06-28
US63/215,660 2021-06-28
PCT/US2022/035068 WO2023278293A1 (en) 2021-06-28 2022-06-27 Methods, compositions, and kits for assay signal amplification

Publications (2)

Publication Number Publication Date
JP2024526217A true JP2024526217A (ja) 2024-07-17
JPWO2023278293A5 JPWO2023278293A5 (enExample) 2025-07-07

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JP2023579630A Pending JP2024526217A (ja) 2021-06-28 2022-06-27 アッセイシグナル増幅のための方法、組成物及びキット

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Country Link
US (1) US20230020070A1 (enExample)
EP (1) EP4363857A1 (enExample)
JP (1) JP2024526217A (enExample)
KR (1) KR20240051109A (enExample)
CN (1) CN117999482A (enExample)
AU (1) AU2022304570A1 (enExample)
CA (1) CA3225576A1 (enExample)
IL (1) IL309517A (enExample)
TW (1) TW202317992A (enExample)
WO (1) WO2023278293A1 (enExample)

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US4442204A (en) 1981-04-10 1984-04-10 Miles Laboratories, Inc. Homogeneous specific binding assay device and preformed complex method
US5310687A (en) 1984-10-31 1994-05-10 Igen, Inc. Luminescent metal chelate labels and means for detection
US6316607B1 (en) 1986-04-30 2001-11-13 Igen International, Inc. Electrochemiluminescent assays
US5591581A (en) 1986-04-30 1997-01-07 Igen, Inc. Electrochemiluminescent rhenium moieties and methods for their use
US5028535A (en) 1989-01-10 1991-07-02 Biosite Diagnostics, Inc. Threshold ligand-receptor assay
US5786141A (en) 1994-08-26 1998-07-28 Bard; Allen J. Electrogenerated chemiluminescence labels for analysis and/or referencing
US5679519A (en) * 1995-05-09 1997-10-21 Oprandy; John J. Multi-label complex for enhanced sensitivity in electrochemiluminescence assay
US6136268A (en) 1999-08-17 2000-10-24 Orion Diagnostica Method for luminescence measurements
US6808939B2 (en) 2001-06-29 2004-10-26 Igen International, Inc. ECL labels having improved non-specific binding properties, methods of using and kits containing the same
DK2461156T3 (da) 2001-06-29 2020-08-03 Meso Scale Technologies Llc Indretning til luminescenstestmålinger
EP1436620B1 (en) * 2001-09-10 2014-04-23 Meso Scale Technologies, LLC Methods and apparatus for conducting multiple measurements on a sample
CA2772050C (en) 2002-12-26 2016-09-06 Meso Scale Technologies, Llc. Assay cartridges and methods of using the same
US7981362B2 (en) 2003-11-04 2011-07-19 Meso Scale Technologies, Llc Modular assay plates, reader systems and methods for test measurements
EP1766400B1 (en) 2004-06-03 2009-04-15 Meso Scale Technologies, LLC Methods for conducting whole blood assays
AU2006330913B2 (en) 2005-12-21 2011-10-27 Meso Scale Technologies, Llc Assay modules having assay reagents and methods of making and using same
JP5955218B2 (ja) 2009-07-27 2016-07-20 メソ スケール テクノロジーズ エルエルシー アッセイ情報管理方法及びデバイス
CA2782694C (en) 2009-12-07 2018-03-27 Meso Scale Technologies, Llc. Assay cartridges and methods of using the same
WO2012015809A2 (en) 2010-07-27 2012-02-02 Xinri Cong Consumable data management
WO2014008381A2 (en) 2012-07-05 2014-01-09 Meso Scale Technologies, Llc. Assay cartridge valve system
JP6087293B2 (ja) 2011-01-06 2017-03-01 メソ スケール テクノロジーズ エルエルシー アッセイカートリッジ及びその使用方法
ES2622998T3 (es) 2012-08-02 2017-07-10 F. Hoffmann-La Roche Ag Nuevos complejos basados en iridio para EQL
WO2014107576A2 (en) 2013-01-04 2014-07-10 Meso Scale Technologies, Llc Assay apparatuses, methods and reagents
KR102275006B1 (ko) 2013-03-11 2021-07-09 메소 스케일 테크놀러지즈, 엘엘시 다중화 어세이를 수행하기 위한 개선된 방법
US10114015B2 (en) * 2013-03-13 2018-10-30 Meso Scale Technologies, Llc. Assay methods
US9618510B2 (en) * 2013-03-13 2017-04-11 Meso Scale Technologies, Llc. Assay methods
AU2015259048B2 (en) * 2014-05-15 2021-09-09 Meso Scale Technologies, Llc. Improved assay methods
CA3129007A1 (en) 2015-04-06 2016-10-13 Eli N. Glezer High throughput system for performing assays using electrochemiluminescence including a consumable shaking apparatus
CA2992983C (en) 2015-07-23 2022-10-25 Meso Scale Technologies, Llc. Integrated consumable data management system & platform
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AU2019418498A1 (en) 2019-01-03 2021-07-15 Meso Scale Technologies, Llc Compositions and methods for carrying out assay measurements

Also Published As

Publication number Publication date
EP4363857A1 (en) 2024-05-08
IL309517A (en) 2024-02-01
US20230020070A1 (en) 2023-01-19
AU2022304570A1 (en) 2024-01-18
WO2023278293A1 (en) 2023-01-05
TW202317992A (zh) 2023-05-01
CN117999482A (zh) 2024-05-07
KR20240051109A (ko) 2024-04-19
CA3225576A1 (en) 2023-01-05

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