CA3202170A1 - Emulsion chemistry and assays for encapsulated droplets - Google Patents
Emulsion chemistry and assays for encapsulated dropletsInfo
- Publication number
- CA3202170A1 CA3202170A1 CA3202170A CA3202170A CA3202170A1 CA 3202170 A1 CA3202170 A1 CA 3202170A1 CA 3202170 A CA3202170 A CA 3202170A CA 3202170 A CA3202170 A CA 3202170A CA 3202170 A1 CA3202170 A1 CA 3202170A1
- Authority
- CA
- Canada
- Prior art keywords
- droplets
- emulsion
- capsules
- phase
- fluorinated
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6846—Common amplification features
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Immunology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Genetics & Genomics (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Manufacturing Of Micro-Capsules (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (21)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US30984510P | 2010-03-02 | 2010-03-02 | |
| US61/309,845 | 2010-03-02 | ||
| US34121810P | 2010-03-25 | 2010-03-25 | |
| US31763510P | 2010-03-25 | 2010-03-25 | |
| US61/317,635 | 2010-03-25 | ||
| US61/341,218 | 2010-03-25 | ||
| US38098110P | 2010-09-08 | 2010-09-08 | |
| US61/380,981 | 2010-09-08 | ||
| US40910610P | 2010-11-01 | 2010-11-01 | |
| US61/409,106 | 2010-11-01 | ||
| US40947310P | 2010-11-02 | 2010-11-02 | |
| US61/409,473 | 2010-11-02 | ||
| US41076910P | 2010-11-05 | 2010-11-05 | |
| US61/410,769 | 2010-11-05 | ||
| US41724110P | 2010-11-25 | 2010-11-25 | |
| US61/417,241 | 2010-11-25 | ||
| US12/976,827 US9598725B2 (en) | 2010-03-02 | 2010-12-22 | Emulsion chemistry for encapsulated droplets |
| US12/976,816 | 2010-12-22 | ||
| US12/976,827 | 2010-12-22 | ||
| US12/976,816 US8399198B2 (en) | 2010-03-02 | 2010-12-22 | Assays with droplets transformed into capsules |
| CA2996219A CA2996219C (en) | 2010-03-02 | 2011-03-02 | Emulsion chemistry and assays for encapsulated droplets |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2996219A Division CA2996219C (en) | 2010-03-02 | 2011-03-02 | Emulsion chemistry and assays for encapsulated droplets |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA3202170A1 true CA3202170A1 (en) | 2011-09-09 |
Family
ID=44531671
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA3202170A Pending CA3202170A1 (en) | 2010-03-02 | 2011-03-02 | Emulsion chemistry and assays for encapsulated droplets |
| CA2824444A Active CA2824444C (en) | 2010-03-02 | 2011-03-02 | Emulsion chemistry and assays for encapsulated droplets |
| CA2996219A Active CA2996219C (en) | 2010-03-02 | 2011-03-02 | Emulsion chemistry and assays for encapsulated droplets |
Family Applications After (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2824444A Active CA2824444C (en) | 2010-03-02 | 2011-03-02 | Emulsion chemistry and assays for encapsulated droplets |
| CA2996219A Active CA2996219C (en) | 2010-03-02 | 2011-03-02 | Emulsion chemistry and assays for encapsulated droplets |
Country Status (5)
| Country | Link |
|---|---|
| US (6) | US9598725B2 (enExample) |
| EP (4) | EP2542660B1 (enExample) |
| JP (2) | JP5963683B2 (enExample) |
| CA (3) | CA3202170A1 (enExample) |
| WO (1) | WO2011109546A1 (enExample) |
Families Citing this family (63)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8709762B2 (en) | 2010-03-02 | 2014-04-29 | Bio-Rad Laboratories, Inc. | System for hot-start amplification via a multiple emulsion |
| US9156010B2 (en) | 2008-09-23 | 2015-10-13 | Bio-Rad Laboratories, Inc. | Droplet-based assay system |
| US8633015B2 (en) * | 2008-09-23 | 2014-01-21 | Bio-Rad Laboratories, Inc. | Flow-based thermocycling system with thermoelectric cooler |
| US20130084572A1 (en) * | 2011-09-30 | 2013-04-04 | Quantalife, Inc. | Calibrations and controls for droplet-based assays |
| US9132394B2 (en) | 2008-09-23 | 2015-09-15 | Bio-Rad Laboratories, Inc. | System for detection of spaced droplets |
| US9399215B2 (en) | 2012-04-13 | 2016-07-26 | Bio-Rad Laboratories, Inc. | Sample holder with a well having a wicking promoter |
| US8663920B2 (en) | 2011-07-29 | 2014-03-04 | Bio-Rad Laboratories, Inc. | Library characterization by digital assay |
| US11130128B2 (en) | 2008-09-23 | 2021-09-28 | Bio-Rad Laboratories, Inc. | Detection method for a target nucleic acid |
| GB2502409B8 (en) | 2008-09-23 | 2014-10-15 | Bio Rad Laboratories | Droplet-based assay system |
| US9417190B2 (en) | 2008-09-23 | 2016-08-16 | Bio-Rad Laboratories, Inc. | Calibrations and controls for droplet-based assays |
| US20110159499A1 (en) * | 2009-11-25 | 2011-06-30 | Quantalife, Inc. | Methods and compositions for detecting genetic material |
| US8951939B2 (en) | 2011-07-12 | 2015-02-10 | Bio-Rad Laboratories, Inc. | Digital assays with multiplexed detection of two or more targets in the same optical channel |
| US9764322B2 (en) | 2008-09-23 | 2017-09-19 | Bio-Rad Laboratories, Inc. | System for generating droplets with pressure monitoring |
| WO2011120020A1 (en) | 2010-03-25 | 2011-09-29 | Quantalife, Inc. | Droplet transport system for detection |
| US12090480B2 (en) | 2008-09-23 | 2024-09-17 | Bio-Rad Laboratories, Inc. | Partition-based method of analysis |
| US9598725B2 (en) * | 2010-03-02 | 2017-03-21 | Bio-Rad Laboratories, Inc. | Emulsion chemistry for encapsulated droplets |
| US12162008B2 (en) | 2008-09-23 | 2024-12-10 | Bio-Rad Laboratories, Inc. | Partition-based method of analysis |
| US10512910B2 (en) | 2008-09-23 | 2019-12-24 | Bio-Rad Laboratories, Inc. | Droplet-based analysis method |
| US9492797B2 (en) | 2008-09-23 | 2016-11-15 | Bio-Rad Laboratories, Inc. | System for detection of spaced droplets |
| US9089844B2 (en) | 2010-11-01 | 2015-07-28 | Bio-Rad Laboratories, Inc. | System for forming emulsions |
| CA2767056C (en) | 2009-09-02 | 2018-12-04 | Bio-Rad Laboratories, Inc. | System for mixing fluids by coalescence of multiple emulsions |
| JP2013524169A (ja) | 2010-03-25 | 2013-06-17 | クァンタライフ・インコーポレーテッド | 液滴によるアッセイ用の検出システム |
| JP2013524171A (ja) | 2010-03-25 | 2013-06-17 | クァンタライフ・インコーポレーテッド | 液滴ベースのアッセイのための液滴の発生 |
| EP3483285B1 (en) | 2011-02-09 | 2021-07-14 | Bio-Rad Laboratories, Inc. | Analysis of nucleic acids |
| US12097495B2 (en) | 2011-02-18 | 2024-09-24 | Bio-Rad Laboratories, Inc. | Methods and compositions for detecting genetic material |
| CA2830443C (en) | 2011-03-18 | 2021-11-16 | Bio-Rad Laboratories, Inc. | Multiplexed digital assays with combinatorial use of signals |
| JP2014512826A (ja) | 2011-04-25 | 2014-05-29 | バイオ−ラド ラボラトリーズ インコーポレイテッド | 核酸分析のための方法および組成物 |
| US10156509B2 (en) | 2011-06-17 | 2018-12-18 | Life Technologies Corporation | Flat-field imaging system and methods of use |
| EP2760578B1 (en) | 2011-09-28 | 2020-08-26 | President and Fellows of Harvard College | Systems and methods for droplet production and/or fluidic manipulation |
| CA2863121A1 (en) | 2011-12-30 | 2013-07-04 | Abbott Molecular Inc. | Microorganism nucleic acid purification from host samples |
| WO2013165579A1 (en) * | 2012-04-30 | 2013-11-07 | Life Technologies Corporation | Polymer-based emulsion breaking methods |
| US9527049B2 (en) | 2012-06-20 | 2016-12-27 | Bio-Rad Laboratories, Inc. | Stabilized droplets for calibration and testing |
| US9422602B2 (en) | 2012-08-15 | 2016-08-23 | Bio-Rad Laboratories, Inc. | Methods and compositions for determining nucleic acid degradation |
| WO2014039587A1 (en) * | 2012-09-05 | 2014-03-13 | Bio-Rad Laboratories, Inc. | Systems and methods for stabilizing droplets |
| US9427737B2 (en) | 2012-12-14 | 2016-08-30 | Bio-Rad Laboratories, Inc. | Methods and compositions for using oils for analysis and detection of molecules |
| US9523116B2 (en) | 2013-02-01 | 2016-12-20 | Bio-Rad Laboratories, Inc. | Multiplexed digital assay with data exclusion for calculation of target levels |
| US9914957B2 (en) * | 2013-02-22 | 2018-03-13 | Bio-Rad Laboratories, Inc. | Devices, systems and methods for thermal control of droplet detection |
| JP2014223608A (ja) * | 2013-04-17 | 2014-12-04 | 積水化学工業株式会社 | マイクロカプセルの製造方法及びマイクロカプセル |
| EP3142790B8 (en) * | 2014-05-14 | 2019-04-24 | Hooke Bio Limited | Method for handling fluid in a microfluidic device with channel plates |
| WO2015189336A1 (en) | 2014-06-11 | 2015-12-17 | Samplix S.A.R.L. | Nucleotide sequence exclusion enrichment by droplet sorting (needls) |
| US10662470B2 (en) | 2015-03-16 | 2020-05-26 | Luminex Corporation | Apparatus and methods for multi-step channel emulsification |
| US11123740B2 (en) | 2015-06-29 | 2021-09-21 | Arizona Board Of Regents On Behalf Of Arizona State University | Systems and methods for continuous flow digital droplet polymerase chain reaction bioanalysis |
| EP3400298B1 (en) | 2016-01-08 | 2024-03-06 | Bio-Rad Laboratories, Inc. | Multiple beads per droplet resolution |
| WO2017130059A1 (en) | 2016-01-25 | 2017-08-03 | Bio-Rad Innovations | Digital microbiology |
| GB2551197B (en) * | 2016-06-10 | 2019-03-13 | Acergy France SAS | Controlling the buoyancy of a mass of buoyant spheres |
| CN110740813B (zh) | 2016-11-28 | 2022-06-03 | 亚利桑那州立大学董事会 | 涉及连续流动液滴反应的系统和方法 |
| US11118217B2 (en) | 2017-01-30 | 2021-09-14 | Bio-Rad Laboratories, Inc. | Emulsion compositions and methods of their use |
| EP3625353B1 (en) | 2017-05-18 | 2022-11-30 | 10X Genomics, Inc. | Methods and systems for sorting droplets and beads |
| CN110799679A (zh) * | 2017-06-20 | 2020-02-14 | 10X基因组学有限公司 | 用于改善液滴稳定的方法和系统 |
| CN110769936B (zh) | 2017-06-21 | 2022-07-08 | 光投发现有限公司 | 微流体分析设备 |
| KR20240017985A (ko) | 2017-06-21 | 2024-02-08 | 베이스4 이노베이션 엘티디 | 마이크로 액적 조작 장치 |
| WO2019012544A1 (en) * | 2017-07-13 | 2019-01-17 | Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. | STRATEGY FOR DOUBLE PROBE DROPLETS FOR SPECIFIC DETECTION OF CIRCULATING DNA MOLECULES SPECIFIC TO A TISSUE |
| US10549279B2 (en) | 2017-08-22 | 2020-02-04 | 10X Genomics, Inc. | Devices having a plurality of droplet formation regions |
| WO2019083852A1 (en) | 2017-10-26 | 2019-05-02 | 10X Genomics, Inc. | MICROFLUIDIC CHANNEL NETWORKS FOR PARTITIONING |
| EP3505933A1 (en) | 2017-12-29 | 2019-07-03 | Blink AG | A microcapsule for detecting and/or quantitating an analyte in a sample |
| CN112236218B (zh) | 2018-04-02 | 2022-04-26 | 滴管公司 | 用于连续流乳液处理的系统和方法 |
| PL3693086T3 (pl) * | 2019-02-08 | 2021-04-19 | Lightcast Discovery Ltd | Sposób manipulacji mikrokroplami |
| AU2020287895A1 (en) * | 2019-06-03 | 2021-12-23 | Dropworks, Inc. | Systems and methods for stabilizing emulsions |
| WO2022087268A1 (en) | 2020-10-21 | 2022-04-28 | Bio-Rad Laboratories, Inc. | Detection of pathogens in wastewater |
| CN113413936B (zh) * | 2021-08-24 | 2021-11-30 | 北京寻因生物科技有限公司 | 一种流量调节结构及微流体芯片 |
| CN113956968B (zh) * | 2021-10-20 | 2023-11-14 | 西安天隆科技有限公司 | 一种液滴型数字化pcr系统及其实现绝对定量化的分析方法 |
| CN114177964B (zh) * | 2022-01-18 | 2023-01-31 | 军事科学院系统工程研究院卫勤保障技术研究所 | 一种平面单层微小液滴阵列的制备方法及其应用 |
| JP2026083466A (ja) * | 2023-03-29 | 2026-05-20 | 国立研究開発法人産業技術総合研究所 | 核酸検出方法並びにヒドロゲルカプセル及びその分散液 |
Family Cites Families (275)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3575220A (en) * | 1968-08-12 | 1971-04-20 | Scientific Industries | Apparatus for dispensing liquid sample |
| BR7500831A (pt) | 1974-02-11 | 1975-12-02 | Fmc Corp | Aparelho de separacao de bolhas de gas corpo de transmissao de gas para uso no aparelho processo para fabricacao do dito corpo e para difusao de bolhas finas de gas em um corpo de liquido |
| US4051025A (en) * | 1976-09-29 | 1977-09-27 | The United States Of America As Represented By The Department Of Health, Education And Welfare | Preparative countercurrent chromatography with a slowly rotating helical tube array |
| US4201691A (en) * | 1978-01-16 | 1980-05-06 | Exxon Research & Engineering Co. | Liquid membrane generator |
| US4348111A (en) * | 1978-12-07 | 1982-09-07 | The English Electric Company Limited | Optical particle analyzers |
| US4283262A (en) * | 1980-07-01 | 1981-08-11 | Instrumentation Laboratory Inc. | Analysis system |
| GB2097692B (en) | 1981-01-10 | 1985-05-22 | Shaw Stewart P D | Combining chemical reagents |
| US4399219A (en) | 1981-01-29 | 1983-08-16 | Massachusetts Institute Of Technology | Process for isolating microbiologically active material |
| US4636075A (en) * | 1984-08-22 | 1987-01-13 | Particle Measuring Systems, Inc. | Particle measurement utilizing orthogonally polarized components of a laser beam |
| US4948961A (en) * | 1985-08-05 | 1990-08-14 | Biotrack, Inc. | Capillary flow device |
| IL82308A (en) * | 1986-06-26 | 1990-11-29 | Ausimont Spa | Microemulsions containing perfluoropolyethers |
| US5225332A (en) * | 1988-04-22 | 1993-07-06 | Massachusetts Institute Of Technology | Process for manipulation of non-aqueous surrounded microdroplets |
| US5055390A (en) | 1988-04-22 | 1991-10-08 | Massachusetts Institute Of Technology | Process for chemical manipulation of non-aqueous surrounded microdroplets |
| JPH082416B2 (ja) | 1988-09-29 | 1996-01-17 | 宮崎県 | エマルションの製造方法 |
| ES2060184T3 (es) * | 1989-06-22 | 1994-11-16 | Atta | Moleculas anfifilicas conteniendo fluor y fosforo con propiedades tensoactivas. |
| GB8917963D0 (en) * | 1989-08-05 | 1989-09-20 | Scras | Apparatus for repeated automatic execution of a thermal cycle for treatment of biological samples |
| GB9016163D0 (en) | 1990-07-24 | 1990-09-05 | Cemu Bioteknik | Chemical method |
| KR100236506B1 (ko) * | 1990-11-29 | 2000-01-15 | 퍼킨-엘머시터스인스트루먼츠 | 폴리머라제 연쇄 반응 수행 장치 |
| US5270183A (en) | 1991-02-08 | 1993-12-14 | Beckman Research Institute Of The City Of Hope | Device and method for the automated cycling of solutions between two or more temperatures |
| US5994056A (en) | 1991-05-02 | 1999-11-30 | Roche Molecular Systems, Inc. | Homogeneous methods for nucleic acid amplification and detection |
| EP0519338B1 (en) * | 1991-06-20 | 1996-08-28 | F. Hoffmann-La Roche Ag | Improved methods for nucleic acid amplification |
| US5422277A (en) * | 1992-03-27 | 1995-06-06 | Ortho Diagnostic Systems Inc. | Cell fixative composition and method of staining cells without destroying the cell surface |
| US5587128A (en) | 1992-05-01 | 1996-12-24 | The Trustees Of The University Of Pennsylvania | Mesoscale polynucleotide amplification devices |
| US5637469A (en) * | 1992-05-01 | 1997-06-10 | Trustees Of The University Of Pennsylvania | Methods and apparatus for the detection of an analyte utilizing mesoscale flow systems |
| US5639423A (en) | 1992-08-31 | 1997-06-17 | The Regents Of The University Of Calfornia | Microfabricated reactor |
| DE69429038T2 (de) * | 1993-07-28 | 2002-03-21 | Pe Corporation (Ny), Norwalk | Vorrichtung und Verfahren zur Nukleinsäurevervielfältigung |
| US5538667A (en) * | 1993-10-28 | 1996-07-23 | Whitehill Oral Technologies, Inc. | Ultramulsions |
| US5538848A (en) * | 1994-11-16 | 1996-07-23 | Applied Biosystems Division, Perkin-Elmer Corp. | Method for detecting nucleic acid amplification using self-quenching fluorescence probe |
| JP2909216B2 (ja) * | 1994-04-29 | 1999-06-23 | パーキン‐エルマー コーポレイション | 核酸増幅生成物のリアルタイム検出装置 |
| US5972716A (en) | 1994-04-29 | 1999-10-26 | The Perkin-Elmer Corporation | Fluorescence monitoring device with textured optical tube and method for reducing background fluorescence |
| DE69527585T2 (de) * | 1994-06-08 | 2003-04-03 | Affymetrix, Inc. | Verfahren und Vorrichtung zum Verpacken von Chips |
| US5555191A (en) * | 1994-10-12 | 1996-09-10 | Trustees Of Columbia University In The City Of New York | Automated statistical tracker |
| FI100859B (sv) | 1994-10-14 | 1998-03-13 | Bjarne Holmbom | Förfarande och anordning för att utföra on-line flödesextraktion av ex traherbara komponenter i vätskor |
| US5585069A (en) | 1994-11-10 | 1996-12-17 | David Sarnoff Research Center, Inc. | Partitioned microelectronic and fluidic device array for clinical diagnostics and chemical synthesis |
| FR2733766B1 (fr) * | 1995-05-03 | 1997-06-06 | Rhone Poulenc Rorer Sa | Methode de diagnostic de la schizophrenie |
| US5856174A (en) * | 1995-06-29 | 1999-01-05 | Affymetrix, Inc. | Integrated nucleic acid diagnostic device |
| US20020022261A1 (en) * | 1995-06-29 | 2002-02-21 | Anderson Rolfe C. | Miniaturized genetic analysis systems and methods |
| US6130098A (en) | 1995-09-15 | 2000-10-10 | The Regents Of The University Of Michigan | Moving microdroplets |
| US6057149A (en) * | 1995-09-15 | 2000-05-02 | The University Of Michigan | Microscale devices and reactions in microscale devices |
| US20020068357A1 (en) * | 1995-09-28 | 2002-06-06 | Mathies Richard A. | Miniaturized integrated nucleic acid processing and analysis device and method |
| US6562605B1 (en) | 1995-11-13 | 2003-05-13 | Genencor International, Inc. | Extraction of water soluble biomaterials from fluids using a carbon dioxide/surfactant mixture |
| US5736314A (en) * | 1995-11-16 | 1998-04-07 | Microfab Technologies, Inc. | Inline thermo-cycler |
| EP0889751B1 (en) | 1996-04-03 | 1999-09-08 | The Perkin-Elmer Corporation | Device and method for multiple analyte detection |
| CN1173776C (zh) * | 1996-06-28 | 2004-11-03 | 卡钳技术有限公司 | 在微规模流体性设备里的高通过量的筛选分析系统 |
| JP3361530B2 (ja) * | 1996-06-28 | 2003-01-07 | カリパー・テクノロジーズ・コープ． | 電子ピペッタおよび電気泳動バイアスのための補償手段 |
| US7026468B2 (en) | 1996-07-19 | 2006-04-11 | Valentis, Inc. | Process and equipment for plasmid purification |
| US6558916B2 (en) * | 1996-08-02 | 2003-05-06 | Axiom Biotechnologies, Inc. | Cell flow apparatus and method for real-time measurements of patient cellular responses |
| GB9621357D0 (en) | 1996-10-12 | 1996-12-04 | Central Research Lab Ltd | Heating apparatus |
| US7268179B2 (en) | 1997-02-03 | 2007-09-11 | Cytonix Corporation | Hydrophobic coating compositions, articles coated with said compositions, and processes for manufacturing same |
| JP2001517948A (ja) | 1997-04-01 | 2001-10-09 | グラクソ、グループ、リミテッド | 核酸配列決定法 |
| EP2327797B1 (en) | 1997-04-01 | 2015-11-25 | Illumina Cambridge Limited | Method of nucleic acid sequencing |
| US6391622B1 (en) * | 1997-04-04 | 2002-05-21 | Caliper Technologies Corp. | Closed-loop biochemical analyzers |
| US6143496A (en) | 1997-04-17 | 2000-11-07 | Cytonix Corporation | Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly |
| JP2002503336A (ja) * | 1997-05-16 | 2002-01-29 | アルバータ リサーチ カウンシル | 微量流通システムおよびその使用方法 |
| JP3120453B2 (ja) | 1997-06-19 | 2000-12-25 | トヨタ自動車株式会社 | 微小液滴の保持方法、反応方法 |
| US5912945A (en) * | 1997-06-23 | 1999-06-15 | Regents Of The University Of California | X-ray compass for determining device orientation |
| ATE358177T1 (de) | 1997-07-07 | 2007-04-15 | Medical Res Council | Ein in vitro sortierverfahren |
| US5980936A (en) | 1997-08-07 | 1999-11-09 | Alliance Pharmaceutical Corp. | Multiple emulsions comprising a hydrophobic continuous phase |
| ES2243007T3 (es) * | 1997-09-16 | 2005-11-16 | Egea Biosciences Llc | Sintesis quimica completa y sintesis de genes de genomas. |
| US6540895B1 (en) * | 1997-09-23 | 2003-04-01 | California Institute Of Technology | Microfabricated cell sorter for chemical and biological materials |
| US6833242B2 (en) | 1997-09-23 | 2004-12-21 | California Institute Of Technology | Methods for detecting and sorting polynucleotides based on size |
| US6074725A (en) * | 1997-12-10 | 2000-06-13 | Caliper Technologies Corp. | Fabrication of microfluidic circuits by printing techniques |
| ES2277430T3 (es) | 1998-03-04 | 2007-07-01 | Visx Incorporated | Sistema de tratamiento con laser de la presbicia. |
| US6175669B1 (en) * | 1998-03-30 | 2001-01-16 | The Regents Of The Universtiy Of California | Optical coherence domain reflectometry guidewire |
| JP3081880B2 (ja) * | 1998-03-30 | 2000-08-28 | 農林水産省食品総合研究所長 | マイクロスフィアの連続製造装置 |
| US6384915B1 (en) * | 1998-03-30 | 2002-05-07 | The Regents Of The University Of California | Catheter guided by optical coherence domain reflectometry |
| JP3012608B1 (ja) * | 1998-09-17 | 2000-02-28 | 農林水産省食品総合研究所長 | マイクロチャネル装置及び同装置を用いたエマルションの製造方法 |
| US6146103A (en) | 1998-10-09 | 2000-11-14 | The Regents Of The University Of California | Micromachined magnetohydrodynamic actuators and sensors |
| US6176609B1 (en) * | 1998-10-13 | 2001-01-23 | V & P Scientific, Inc. | Magnetic tumble stirring method, devices and machines for mixing in vessels |
| US6637463B1 (en) | 1998-10-13 | 2003-10-28 | Biomicro Systems, Inc. | Multi-channel microfluidic system design with balanced fluid flow distribution |
| US6086740A (en) | 1998-10-29 | 2000-07-11 | Caliper Technologies Corp. | Multiplexed microfluidic devices and systems |
| US6261431B1 (en) * | 1998-12-28 | 2001-07-17 | Affymetrix, Inc. | Process for microfabrication of an integrated PCR-CE device and products produced by the same |
| GB9900298D0 (en) | 1999-01-07 | 1999-02-24 | Medical Res Council | Optical sorting method |
| US6522407B2 (en) * | 1999-01-22 | 2003-02-18 | The Regents Of The University Of California | Optical detection dental disease using polarized light |
| US6326083B1 (en) | 1999-03-08 | 2001-12-04 | Calipher Technologies Corp. | Surface coating for microfluidic devices that incorporate a biopolymer resistant moiety |
| US6303343B1 (en) | 1999-04-06 | 2001-10-16 | Caliper Technologies Corp. | Inefficient fast PCR |
| US6964846B1 (en) | 1999-04-09 | 2005-11-15 | Exact Sciences Corporation | Methods for detecting nucleic acids indicative of cancer |
| US6357907B1 (en) * | 1999-06-15 | 2002-03-19 | V & P Scientific, Inc. | Magnetic levitation stirring devices and machines for mixing in vessels |
| US6977145B2 (en) | 1999-07-28 | 2005-12-20 | Serono Genetics Institute S.A. | Method for carrying out a biochemical protocol in continuous flow in a microreactor |
| WO2001007159A2 (en) | 1999-07-28 | 2001-02-01 | Genset | Integration of biochemical protocols in a continuous flow microfluidic device |
| US6440706B1 (en) * | 1999-08-02 | 2002-08-27 | Johns Hopkins University | Digital amplification |
| US6524456B1 (en) * | 1999-08-12 | 2003-02-25 | Ut-Battelle, Llc | Microfluidic devices for the controlled manipulation of small volumes |
| DE19964337B4 (de) * | 1999-10-01 | 2004-09-16 | Agilent Technologies, Inc. (n.d.Ges.d.Staates Delaware), Palo Alto | Mikrofluidischer Mikrochip mit abbiegbarem Ansaugrohr |
| ATE458831T1 (de) * | 1999-12-07 | 2010-03-15 | Exact Sciences Corp | Verfahren zum nachweis von lungenneoplasmen in fäkalen proben |
| ATE340025T1 (de) * | 2000-01-06 | 2006-10-15 | Caliper Life Sciences Inc | Vorrichtungen und verfahren für hochdurchsatz- probenentnahme und analyse |
| US20020151040A1 (en) | 2000-02-18 | 2002-10-17 | Matthew O' Keefe | Apparatus and methods for parallel processing of microvolume liquid reactions |
| JP2003522963A (ja) * | 2000-02-18 | 2003-07-29 | アクララ バイオサイエンシーズ， インコーポレイテッド | 複数部位反応デバイスおよび方法 |
| JP3282619B2 (ja) | 2000-03-22 | 2002-05-20 | 住友電装株式会社 | ワイヤーハーネスの屈曲試験方法及び電線の屈曲試験装置 |
| US20010048900A1 (en) | 2000-05-24 | 2001-12-06 | Bardell Ronald L. | Jet vortex mixer |
| US7351376B1 (en) * | 2000-06-05 | 2008-04-01 | California Institute Of Technology | Integrated active flux microfluidic devices and methods |
| KR100854530B1 (ko) | 2000-06-05 | 2008-08-26 | 신젠타 리미티드 | 신규한 마이크로캡슐 |
| US6466713B2 (en) * | 2000-08-18 | 2002-10-15 | The Regents Of The University Of California | Optical fiber head for providing lateral viewing |
| US6773566B2 (en) * | 2000-08-31 | 2004-08-10 | Nanolytics, Inc. | Electrostatic actuators for microfluidics and methods for using same |
| ATE448875T1 (de) | 2000-09-14 | 2009-12-15 | Caliper Life Sciences Inc | Mikrofluidische vorrichtungen und methoden um temperatur-vermittelte reaktionen durchzuführen |
| US7294503B2 (en) | 2000-09-15 | 2007-11-13 | California Institute Of Technology | Microfabricated crossflow devices and methods |
| US20020142483A1 (en) | 2000-10-30 | 2002-10-03 | Sequenom, Inc. | Method and apparatus for delivery of submicroliter volumes onto a substrate |
| KR100435921B1 (ko) | 2000-12-29 | 2004-06-12 | 주식회사 태평양 | 동수역학적 이중 안정화에 의한 안정한 수-유-수 다중에멀젼 시스템 및 이의 제조방법 |
| KR100860291B1 (ko) | 2001-01-19 | 2008-09-25 | 에게아 바이오사이언시스, 인크. | 표적 폴리펩티드를 암호화하는 폴리뉴클레오티드의 컴퓨터지시된 어셈블리 |
| US7567596B2 (en) * | 2001-01-30 | 2009-07-28 | Board Of Trustees Of Michigan State University | Control system and apparatus for use with ultra-fast laser |
| EP1721658B2 (en) * | 2001-02-23 | 2020-08-05 | Japan Science and Technology Agency | Process and apparatus for producing microcapsules |
| US6949176B2 (en) * | 2001-02-28 | 2005-09-27 | Lightwave Microsystems Corporation | Microfluidic control using dielectric pumping |
| US7270786B2 (en) | 2001-03-28 | 2007-09-18 | Handylab, Inc. | Methods and systems for processing microfluidic samples of particle containing fluids |
| US7192557B2 (en) * | 2001-03-28 | 2007-03-20 | Handylab, Inc. | Methods and systems for releasing intracellular material from cells within microfluidic samples of fluids |
| US6575188B2 (en) * | 2001-07-26 | 2003-06-10 | Handylab, Inc. | Methods and systems for fluid control in microfluidic devices |
| US7010391B2 (en) * | 2001-03-28 | 2006-03-07 | Handylab, Inc. | Methods and systems for control of microfluidic devices |
| AU2002307152A1 (en) | 2001-04-06 | 2002-10-21 | California Institute Of Technology | Nucleic acid amplification utilizing microfluidic devices |
| US6793723B2 (en) | 2001-05-10 | 2004-09-21 | Pitney Bowes Inc. | Homogeneous photosensitive optically variable ink compositions for ink jet printing |
| US20030049659A1 (en) * | 2001-05-29 | 2003-03-13 | Lapidus Stanley N. | Devices and methods for isolating samples into subsamples for analysis |
| US20030013680A1 (en) | 2001-06-12 | 2003-01-16 | Keryx | Methods using glycosaminoglycans for the treatment of nephropathy |
| US6905885B2 (en) * | 2001-06-12 | 2005-06-14 | The Regents Of The University Of California | Portable pathogen detection system |
| US6808683B2 (en) * | 2001-09-25 | 2004-10-26 | Cytonome, Inc. | Droplet dispensing system |
| JP4537053B2 (ja) * | 2001-06-25 | 2010-09-01 | ジョージア テック リサーチ コーポレーション | 二重共鳴エネルギー転移核酸プローブ |
| US6797257B2 (en) | 2001-06-26 | 2004-09-28 | The Board Of Trustees Of The University Of Illinois | Paramagnetic polymerized protein microspheres and methods of preparation thereof |
| US6573490B2 (en) * | 2001-06-28 | 2003-06-03 | Valeo Electrical Systems, Inc. | Interleaved mosaic imaging rain sensor |
| US20030032172A1 (en) * | 2001-07-06 | 2003-02-13 | The Regents Of The University Of California | Automated nucleic acid assay system |
| HUP0400164A2 (hu) | 2001-07-12 | 2004-07-28 | Avecia Ltd. | Mikrokapszulázott katalizátor, eljárás előállítására és alkalmazása |
| US7297778B2 (en) * | 2001-07-25 | 2007-11-20 | Affymetrix, Inc. | Complexity management of genomic DNA |
| US20030027150A1 (en) * | 2001-08-03 | 2003-02-06 | Katz David A. | Method of haplotyping and kit therefor |
| AUPR707101A0 (en) | 2001-08-16 | 2001-09-06 | Corbett Research Pty Ltd | Continuous flow thermal device |
| US7094379B2 (en) * | 2001-10-24 | 2006-08-22 | Commissariat A L'energie Atomique | Device for parallel and synchronous injection for sequential injection of different reagents |
| KR100442836B1 (ko) | 2001-11-10 | 2004-08-02 | 삼성전자주식회사 | 생화학 유체를 온도가 다른 폐쇄된 챔버 구간을 따라 회전이동시키는 폐쇄 유체 회로 시스템 |
| GB0127564D0 (en) | 2001-11-16 | 2002-01-09 | Medical Res Council | Emulsion compositions |
| EP1463796B1 (en) | 2001-11-30 | 2013-01-09 | Fluidigm Corporation | Microfluidic device and methods of using same |
| US7198897B2 (en) * | 2001-12-19 | 2007-04-03 | Brandeis University | Late-PCR |
| WO2003057010A2 (en) * | 2002-01-04 | 2003-07-17 | Board Of Regents, The University Of Texas System | Droplet-based microfluidic oligonucleotide synthesis engine |
| US20030180765A1 (en) * | 2002-02-01 | 2003-09-25 | The Johns Hopkins University | Digital amplification for detection of mismatch repair deficient tumor cells |
| DE60303504T2 (de) | 2002-02-22 | 2006-10-12 | Biodot, Inc., Irvine | Verfahren und vorrichtung zur kontaktlosen tröpfchenabgabe unterhalb einer fluidischen oberfläche |
| CN1656234B (zh) * | 2002-03-20 | 2012-02-01 | 创新生物公司 | 包囊化核酸扩增反应混合物的微胶囊及其作为反应隔间进行平行反应的用途 |
| US7312085B2 (en) | 2002-04-01 | 2007-12-25 | Fluidigm Corporation | Microfluidic particle-analysis systems |
| US7993266B2 (en) | 2002-04-26 | 2011-08-09 | Lawrence Livermore National Security, Llc | Early detection of contagious diseases |
| EP1508044B1 (en) | 2002-05-09 | 2010-09-01 | The University of Chicago | Device and method for pressure-driven plug transport and reaction |
| FR2841063B1 (fr) * | 2002-06-18 | 2004-09-17 | Commissariat Energie Atomique | Dispositif de deplacement de petits volumes de liquide le long d'un micro-catenaire par des forces electrostatiques |
| JP2006507921A (ja) * | 2002-06-28 | 2006-03-09 | プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ | 流体分散のための方法および装置 |
| US6794671B2 (en) | 2002-07-17 | 2004-09-21 | Particle Sizing Systems, Inc. | Sensors and methods for high-sensitivity optical particle counting and sizing |
| US7188731B2 (en) * | 2002-08-26 | 2007-03-13 | The Regents Of The University Of California | Variable flexure-based fluid filter |
| US20040038385A1 (en) | 2002-08-26 | 2004-02-26 | Langlois Richard G. | System for autonomous monitoring of bioagents |
| JP5695287B2 (ja) | 2002-10-02 | 2015-04-01 | カリフォルニア インスティテュート オブ テクノロジー | 微小流体の核酸解析 |
| US20060094108A1 (en) | 2002-12-20 | 2006-05-04 | Karl Yoder | Thermal cycler for microfluidic array assays |
| JP4395133B2 (ja) | 2002-12-20 | 2010-01-06 | カリパー・ライフ・サイエンシズ・インク． | Ｄｎａの単一分子増幅および検出 |
| EP1608952B1 (en) | 2002-12-20 | 2016-08-10 | Life Technologies Corporation | Assay apparatus and method using microfluidic arrays |
| US20050042639A1 (en) * | 2002-12-20 | 2005-02-24 | Caliper Life Sciences, Inc. | Single molecule amplification and detection of DNA length |
| DE602004026033D1 (de) * | 2003-01-29 | 2010-04-29 | 454 Corp | Zweiendige sequenzierung |
| WO2004071638A2 (en) | 2003-02-11 | 2004-08-26 | Regents Of The University Of California, The | Microfluidic devices and method for controlled viscous shearing and formation of amphiphilic vesicles |
| US7041481B2 (en) | 2003-03-14 | 2006-05-09 | The Regents Of The University Of California | Chemical amplification based on fluid partitioning |
| DK2308419T3 (da) | 2003-03-28 | 2016-06-06 | Inguran Llc | Fremgangsmåde til bedømmelse af farvningstilstandene for dyresperm, som skal sorteres. |
| US8828663B2 (en) | 2005-03-18 | 2014-09-09 | Fluidigm Corporation | Thermal reaction device and method for using the same |
| US7604965B2 (en) | 2003-04-03 | 2009-10-20 | Fluidigm Corporation | Thermal reaction device and method for using the same |
| DE10315640A1 (de) | 2003-04-04 | 2004-10-14 | Ignatov, Konstantin | Verfahren zur kontrollierten Freisetzung von Komponenten in eine Lösung |
| WO2004094020A2 (en) | 2003-04-17 | 2004-11-04 | Fluidigm Corporation | Crystal growth devices and systems, and methods for using same |
| DE602004028837D1 (de) | 2003-07-03 | 2010-10-07 | Univ New Jersey Med | Gene als diagnostische werkzeuge für autismus |
| US7629123B2 (en) | 2003-07-03 | 2009-12-08 | University Of Medicine And Dentistry Of New Jersey | Compositions and methods for diagnosing autism |
| US8048627B2 (en) | 2003-07-05 | 2011-11-01 | The Johns Hopkins University | Method and compositions for detection and enumeration of genetic variations |
| AU2004262406B2 (en) | 2003-08-01 | 2008-07-03 | Ge Healthcare Bio-Sciences Ab | Optical resonance analysis unit |
| CN1842368B (zh) | 2003-08-27 | 2014-05-28 | 哈佛大学 | 流体物种的电子控制 |
| AU2004270220B2 (en) | 2003-09-05 | 2009-03-05 | The Chinese University Of Hong Kong | Method for non-invasive prenatal diagnosis |
| US20050277125A1 (en) | 2003-10-27 | 2005-12-15 | Massachusetts Institute Of Technology | High-density reaction chambers and methods of use |
| US7141537B2 (en) | 2003-10-30 | 2006-11-28 | 3M Innovative Properties Company | Mixture of fluorinated polyethers and use thereof as surfactant |
| US7277218B2 (en) * | 2003-11-04 | 2007-10-02 | Sipix Imaging, Inc. | Electrophoretic compositions |
| ES2355242T3 (es) | 2003-12-05 | 2011-03-24 | Beatrice And Samuel A. Seaver Foundation | Procedimiento de evaluación del riesgo genético relativo al autismo. |
| WO2005073410A2 (en) | 2004-01-28 | 2005-08-11 | 454 Corporation | Nucleic acid amplification with continuous flow emulsion |
| US8293471B2 (en) | 2004-01-28 | 2012-10-23 | Marshall University Research Corporation | Apparatus and method for a continuous rapid thermal cycle system |
| WO2005075683A1 (en) | 2004-02-03 | 2005-08-18 | Postech Foundation | High throughput device for performing continuous-flow reactions |
| KR100552706B1 (ko) * | 2004-03-12 | 2006-02-20 | 삼성전자주식회사 | 핵산 증폭 방법 및 장치 |
| CN100431679C (zh) | 2004-03-23 | 2008-11-12 | 独立行政法人科学技术振兴机构 | 微小液滴的生成方法及装置 |
| US7432106B2 (en) | 2004-03-24 | 2008-10-07 | Applied Biosystems Inc. | Liquid processing device including gas trap, and system and method |
| EP2439284B1 (en) | 2004-03-31 | 2019-05-08 | The General Hospital Corporation | Method to determine responsiveness of cancer to epidermal growth factor receptor targeting treatments |
| US20050221279A1 (en) | 2004-04-05 | 2005-10-06 | The Regents Of The University Of California | Method for creating chemical sensors using contact-based microdispensing technology |
| HU227246B1 (en) | 2004-04-30 | 2010-12-28 | Szegedi Biolog Koezpont | Use of genes as molecular markers in diagnosis of schizophrenia and diagnostic kit |
| WO2005110395A1 (en) | 2004-05-19 | 2005-11-24 | University Of South Carolina | System and device for magnetic drug targeting with magnetic drug carrier particles |
| WO2006002167A2 (en) | 2004-06-17 | 2006-01-05 | University Of Florida Research Foudation, Inc. | Multi-acceptor molecular probes and applications thereof |
| DE602005013153D1 (de) | 2004-06-29 | 2009-04-23 | Roche Diagnostics Gmbh | Assoziation von SNPs in PPAR gamma mit Osteoporose |
| WO2006023719A2 (en) | 2004-08-20 | 2006-03-02 | Enh Research Institute | Identification of snp’s associated with schizophrenia, schizoaffective disorder and bipolar disorder |
| JP5885901B2 (ja) | 2004-09-09 | 2016-03-16 | アンスティテュート キュリー | マイクロチャネルまたは他のマイクロ容器中でパケットを操作するためのデバイス |
| US7968287B2 (en) | 2004-10-08 | 2011-06-28 | Medical Research Council Harvard University | In vitro evolution in microfluidic systems |
| WO2006047787A2 (en) | 2004-10-27 | 2006-05-04 | Exact Sciences Corporation | Method for monitoring disease progression or recurrence |
| US8951746B2 (en) | 2004-11-05 | 2015-02-10 | Southwest Research Institute | Method and devices for screening cervical cancer |
| US20090098044A1 (en) | 2004-11-15 | 2009-04-16 | Australian Nuclear Science And Technology Organisation | Solid particles from controlled destabilisation of microemulsions |
| US20060166223A1 (en) | 2005-01-26 | 2006-07-27 | Reed Michael W | DNA purification and analysis on nanoengineered surfaces |
| US7423751B2 (en) | 2005-02-08 | 2008-09-09 | Northrop Grumman Corporation | Systems and methods for use in detecting harmful aerosol particles |
| WO2006086777A2 (en) | 2005-02-11 | 2006-08-17 | Memorial Sloan Kettering Cancer Center | Methods and compositions for detecting a drug resistant egfr mutant |
| US9039273B2 (en) | 2005-03-04 | 2015-05-26 | President And Fellows Of Harvard College | Method and apparatus for forming multiple emulsions |
| RU2400538C2 (ru) | 2005-03-05 | 2010-09-27 | Сиджен, Инк. | Олигонуклеотид с двойственной специфичностью и способы, в которых он используется |
| WO2006099579A2 (en) | 2005-03-16 | 2006-09-21 | Applera Corporation | Compositions and methods for clonal amplification and analysis of polynucleotides |
| US20070048756A1 (en) | 2005-04-18 | 2007-03-01 | Affymetrix, Inc. | Methods for whole genome association studies |
| DE102005037401B4 (de) | 2005-08-08 | 2007-09-27 | MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. | Bildung einer Emulsion in einem fluidischen Mikrosystem |
| AU2006294565A1 (en) | 2005-09-30 | 2007-04-05 | Perlegen Sciences, Inc. | Methods and compositions for screening and treatment of disorders of blood glucose regulation |
| WO2007081385A2 (en) | 2006-01-11 | 2007-07-19 | Raindance Technologies, Inc. | Microfluidic devices and methods of use in the formation and control of nanoreactors |
| US7976795B2 (en) | 2006-01-19 | 2011-07-12 | Rheonix, Inc. | Microfluidic systems |
| WO2007089541A2 (en) | 2006-01-27 | 2007-08-09 | President And Fellows Of Harvard College | Fluidic droplet coalescence |
| WO2007092473A2 (en) | 2006-02-02 | 2007-08-16 | The Board Of Trustees Of The Leland Stanford Junior University | Non-invasive fetal genetic screening by digital analysis |
| EP1991357B1 (en) | 2006-02-07 | 2016-09-14 | Stokes Bio Limited | A microfluidic analysis system |
| US20100304446A1 (en) | 2006-02-07 | 2010-12-02 | Stokes Bio Limited | Devices, systems, and methods for amplifying nucleic acids |
| EP2298438A1 (en) | 2006-02-07 | 2011-03-23 | Stokes Bio Limited | A microfluidic droplet queuing network |
| GB0603501D0 (en) | 2006-02-22 | 2006-04-05 | Reaxa Ltd | Catalyst |
| WO2007120745A2 (en) | 2006-04-11 | 2007-10-25 | Optiscan Biomedical Corporation | Noise reduction for analyte detection system |
| US8492168B2 (en) | 2006-04-18 | 2013-07-23 | Advanced Liquid Logic Inc. | Droplet-based affinity assays |
| US7815871B2 (en) | 2006-04-18 | 2010-10-19 | Advanced Liquid Logic, Inc. | Droplet microactuator system |
| US7816121B2 (en) | 2006-04-18 | 2010-10-19 | Advanced Liquid Logic, Inc. | Droplet actuation system and method |
| CA2680532C (en) | 2006-04-18 | 2017-03-21 | Advanced Liquid Logic, Inc. | Droplet-based pyrosequencing |
| US9562837B2 (en) * | 2006-05-11 | 2017-02-07 | Raindance Technologies, Inc. | Systems for handling microfludic droplets |
| WO2007133710A2 (en) | 2006-05-11 | 2007-11-22 | Raindance Technologies, Inc. | Microfluidic devices and methods of use thereof |
| US7608399B2 (en) | 2006-06-26 | 2009-10-27 | Blood Cell Storage, Inc. | Device and method for extraction and analysis of nucleic acids from biological samples |
| EP1881079A1 (en) | 2006-07-20 | 2008-01-23 | Pangaea Biotech, S.A. | Method for the detection of EGFR mutations in blood samples |
| US9012390B2 (en) | 2006-08-07 | 2015-04-21 | Raindance Technologies, Inc. | Fluorocarbon emulsion stabilizing surfactants |
| WO2008024114A1 (en) | 2006-08-24 | 2008-02-28 | Genizon Biosciences Inc. | Genemap of the human genes associated with schizophrenia |
| EP1912067A1 (en) | 2006-10-12 | 2008-04-16 | Eppendorf Array Technologies S.A. | Method for quantification of a target compound obtained from a biological sample upon chips |
| US8841116B2 (en) | 2006-10-25 | 2014-09-23 | The Regents Of The University Of California | Inline-injection microdevice and microfabricated integrated DNA analysis system using same |
| WO2008070074A2 (en) | 2006-12-04 | 2008-06-12 | Pgxhealth Llc | Genetic markers of schizophrenia |
| WO2008070862A2 (en) | 2006-12-07 | 2008-06-12 | Biocept, Inc. | Non-invasive prenatal genetic screen |
| US8262900B2 (en) | 2006-12-14 | 2012-09-11 | Life Technologies Corporation | Methods and apparatus for measuring analytes using large scale FET arrays |
| US8338166B2 (en) | 2007-01-04 | 2012-12-25 | Lawrence Livermore National Security, Llc | Sorting, amplification, detection, and identification of nucleic acid subsequences in a complex mixture |
| WO2008089244A2 (en) | 2007-01-17 | 2008-07-24 | University Of Rochester | Frequency-addressable apparatus and methods for actuation of liquids |
| US20090011417A1 (en) | 2007-03-07 | 2009-01-08 | George Maltezos | Testing Device |
| WO2008109176A2 (en) | 2007-03-07 | 2008-09-12 | President And Fellows Of Harvard College | Assays and other reactions involving droplets |
| WO2008112177A2 (en) | 2007-03-08 | 2008-09-18 | Genizon Biosciences, Inc. | Genemap of the human genes associated with schizophrenia |
| EP2136786B8 (en) * | 2007-03-28 | 2012-11-14 | President and Fellows of Harvard College | Apparatus for forming droplets |
| JP5666763B2 (ja) | 2007-04-11 | 2015-02-12 | 味の素株式会社 | 油中水型乳化組成物 |
| WO2009002920A1 (en) | 2007-06-22 | 2008-12-31 | Advanced Liquid Logic, Inc. | Droplet-based nucleic acid amplification in a temperature gradient |
| US20090068170A1 (en) | 2007-07-13 | 2009-03-12 | President And Fellows Of Harvard College | Droplet-based selection |
| CA2694820C (en) | 2007-07-30 | 2015-06-16 | F. Hoffman-La Roche Ag | Methods of identification using methylation of cpg |
| US20090053719A1 (en) | 2007-08-03 | 2009-02-26 | The Chinese University Of Hong Kong | Analysis of nucleic acids by digital pcr |
| ES2380844T3 (es) | 2007-09-07 | 2012-05-18 | Fluidigm Corporation | Determinación de la variación en el número de copias, métodos y sistemas |
| EP2203567B1 (en) | 2007-10-16 | 2011-05-11 | Roche Diagnostics GmbH | High resolution, high throughput hla genotyping by clonal sequencing |
| US7807920B2 (en) | 2007-10-30 | 2010-10-05 | Opel, Inc. | Concentrated solar photovoltaic module |
| WO2009085246A1 (en) | 2007-12-20 | 2009-07-09 | University Of Massachusetts | Cross-linked biopolymers, related compositions and methods of use |
| JP5224801B2 (ja) | 2007-12-21 | 2013-07-03 | キヤノン株式会社 | 核酸増幅装置 |
| CA2639954C (en) | 2008-02-11 | 2017-08-15 | Aaron R. Wheeler | Droplet-based cell culture and cell assays using digital microfluidics |
| US20090220434A1 (en) | 2008-02-29 | 2009-09-03 | Florida State University Research Foundation | Nanoparticles that facilitate imaging of biological tissue and methods of forming the same |
| US8062903B2 (en) | 2008-03-03 | 2011-11-22 | University Of Washington | Droplet compartmentalization for chemical separation and on-line sampling |
| US9487822B2 (en) | 2008-03-19 | 2016-11-08 | Fluidigm Corporation | Method and apparatus for determining copy number variation using digital PCR |
| US9011777B2 (en) | 2008-03-21 | 2015-04-21 | Lawrence Livermore National Security, Llc | Monodisperse microdroplet generation and stopping without coalescence |
| EP2672260A1 (en) | 2008-05-13 | 2013-12-11 | Advanced Liquid Logic, Inc. | Droplet actuator devices, systems and methods |
| WO2010001419A2 (en) | 2008-07-04 | 2010-01-07 | Decode Genetics Ehf | Copy number variations predictive of risk of schizophrenia |
| EP4047367A1 (en) | 2008-07-18 | 2022-08-24 | Bio-Rad Laboratories, Inc. | Method for detecting target analytes with droplet libraries |
| US8256931B2 (en) | 2008-07-24 | 2012-09-04 | Seward George H | Achromatic homogenizer and collimator for LEDs |
| WO2010018465A2 (en) | 2008-08-12 | 2010-02-18 | Stokes Bio Limited | Methods and devices for digital pcr |
| US9180453B2 (en) | 2008-08-15 | 2015-11-10 | University Of Washington | Method and apparatus for the discretization and manipulation of sample volumes |
| WO2010021936A1 (en) | 2008-08-16 | 2010-02-25 | The Board Of Trustees Of The Leland Stanford Junior University | Digital pcr calibration for high throughput sequencing |
| US8383345B2 (en) | 2008-09-12 | 2013-02-26 | University Of Washington | Sequence tag directed subassembly of short sequencing reads into long sequencing reads |
| US20110218123A1 (en) | 2008-09-19 | 2011-09-08 | President And Fellows Of Harvard College | Creation of libraries of droplets and related species |
| US9598725B2 (en) * | 2010-03-02 | 2017-03-21 | Bio-Rad Laboratories, Inc. | Emulsion chemistry for encapsulated droplets |
| US8951939B2 (en) | 2011-07-12 | 2015-02-10 | Bio-Rad Laboratories, Inc. | Digital assays with multiplexed detection of two or more targets in the same optical channel |
| US8663920B2 (en) | 2011-07-29 | 2014-03-04 | Bio-Rad Laboratories, Inc. | Library characterization by digital assay |
| US20120171683A1 (en) | 2010-03-02 | 2012-07-05 | Ness Kevin D | Analysis of fragmented genomic dna in droplets |
| WO2011120020A1 (en) | 2010-03-25 | 2011-09-29 | Quantalife, Inc. | Droplet transport system for detection |
| US9132394B2 (en) | 2008-09-23 | 2015-09-15 | Bio-Rad Laboratories, Inc. | System for detection of spaced droplets |
| US8633015B2 (en) | 2008-09-23 | 2014-01-21 | Bio-Rad Laboratories, Inc. | Flow-based thermocycling system with thermoelectric cooler |
| US20130084572A1 (en) | 2011-09-30 | 2013-04-04 | Quantalife, Inc. | Calibrations and controls for droplet-based assays |
| US20130059754A1 (en) | 2011-09-01 | 2013-03-07 | Bio-Rad Laboratories, Inc. | Digital assays with reduced measurement uncertainty |
| US9089844B2 (en) | 2010-11-01 | 2015-07-28 | Bio-Rad Laboratories, Inc. | System for forming emulsions |
| US9156010B2 (en) | 2008-09-23 | 2015-10-13 | Bio-Rad Laboratories, Inc. | Droplet-based assay system |
| US8709762B2 (en) | 2010-03-02 | 2014-04-29 | Bio-Rad Laboratories, Inc. | System for hot-start amplification via a multiple emulsion |
| EP2389455A4 (en) | 2009-01-26 | 2012-12-05 | Verinata Health Inc | METHODS AND COMPOSITIONS FOR IDENTIFYING A FETAL CELL |
| WO2010111231A1 (en) | 2009-03-23 | 2010-09-30 | Raindance Technologies, Inc. | Manipulation of microfluidic droplets |
| US20100261230A1 (en) | 2009-04-08 | 2010-10-14 | Applied Biosystems, Llc | System comprising dual-sided thermal cycler and emulsion pcr in pouch |
| WO2011020011A2 (en) * | 2009-08-13 | 2011-02-17 | Advanced Liquid Logic, Inc. | Droplet actuator and droplet-based techniques |
| CA2767056C (en) | 2009-09-02 | 2018-12-04 | Bio-Rad Laboratories, Inc. | System for mixing fluids by coalescence of multiple emulsions |
| CA3041540C (en) | 2009-09-21 | 2021-08-24 | Bio-Rad Laboratories, Inc. | Magnetic lysis method and device |
| US10520500B2 (en) | 2009-10-09 | 2019-12-31 | Abdeslam El Harrak | Labelled silica-based nanomaterial with enhanced properties and uses thereof |
| US9890408B2 (en) | 2009-10-15 | 2018-02-13 | Ibis Biosciences, Inc. | Multiple displacement amplification |
| US8835358B2 (en) | 2009-12-15 | 2014-09-16 | Cellular Research, Inc. | Digital counting of individual molecules by stochastic attachment of diverse labels |
| EP2517025B1 (en) | 2009-12-23 | 2019-11-27 | Bio-Rad Laboratories, Inc. | Methods for reducing the exchange of molecules between droplets |
| US9399797B2 (en) | 2010-02-12 | 2016-07-26 | Raindance Technologies, Inc. | Digital analyte analysis |
| EP4435111A1 (en) | 2010-02-12 | 2024-09-25 | Bio-Rad Laboratories, Inc. | Digital analyte analysis |
| JP2013524169A (ja) | 2010-03-25 | 2013-06-17 | クァンタライフ・インコーポレーテッド | 液滴によるアッセイ用の検出システム |
| JP2013524171A (ja) | 2010-03-25 | 2013-06-17 | クァンタライフ・インコーポレーテッド | 液滴ベースのアッセイのための液滴の発生 |
| WO2012045012A2 (en) | 2010-09-30 | 2012-04-05 | Raindance Technologies, Inc. | Sandwich assays in droplets |
| US9266104B2 (en) | 2011-02-11 | 2016-02-23 | Raindance Technologies, Inc. | Thermocycling device for nucleic acid amplification and methods of use |
| EP3859011A1 (en) | 2011-02-11 | 2021-08-04 | Bio-Rad Laboratories, Inc. | Methods for forming mixed droplets |
| WO2012112804A1 (en) | 2011-02-18 | 2012-08-23 | Raindance Technoligies, Inc. | Compositions and methods for molecular labeling |
| CA2830443C (en) | 2011-03-18 | 2021-11-16 | Bio-Rad Laboratories, Inc. | Multiplexed digital assays with combinatorial use of signals |
| US8841071B2 (en) | 2011-06-02 | 2014-09-23 | Raindance Technologies, Inc. | Sample multiplexing |
| EP2714970B1 (en) | 2011-06-02 | 2017-04-19 | Raindance Technologies, Inc. | Enzyme quantification |
| CN103930886A (zh) | 2011-07-13 | 2014-07-16 | 伯乐生命医学产品有限公司 | 使用副本的meta 分析进行真实世界误差的计算 |
| US8658430B2 (en) | 2011-07-20 | 2014-02-25 | Raindance Technologies, Inc. | Manipulating droplet size |
-
2010
- 2010-12-22 US US12/976,827 patent/US9598725B2/en active Active
- 2010-12-22 US US12/976,816 patent/US8399198B2/en active Active
-
2011
- 2011-03-02 EP EP11751314.3A patent/EP2542660B1/en active Active
- 2011-03-02 WO PCT/US2011/026901 patent/WO2011109546A1/en not_active Ceased
- 2011-03-02 CA CA3202170A patent/CA3202170A1/en active Pending
- 2011-03-02 EP EP19195982.4A patent/EP3636741B1/en active Active
- 2011-03-02 CA CA2824444A patent/CA2824444C/en active Active
- 2011-03-02 CA CA2996219A patent/CA2996219C/en active Active
- 2011-03-02 JP JP2012556218A patent/JP5963683B2/ja active Active
- 2011-03-02 EP EP24198754.4A patent/EP4464794A3/en active Pending
- 2011-03-02 EP EP17166391.7A patent/EP3266862B1/en active Active
-
2016
- 2016-02-09 JP JP2016022474A patent/JP6188844B2/ja active Active
-
2017
- 2017-03-07 US US15/452,582 patent/US10378048B2/en active Active
-
2019
- 2019-06-20 US US16/447,315 patent/US11060136B2/en active Active
-
2021
- 2021-06-11 US US17/345,834 patent/US11866771B2/en active Active
-
2023
- 2023-11-27 US US18/519,957 patent/US20240093280A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| JP5963683B2 (ja) | 2016-08-03 |
| EP3266862A1 (en) | 2018-01-10 |
| US11060136B2 (en) | 2021-07-13 |
| EP2542660B1 (en) | 2017-06-21 |
| CA2996219A1 (en) | 2011-09-09 |
| EP3266862B1 (en) | 2019-10-02 |
| EP3636741B1 (en) | 2024-09-25 |
| US20190316191A1 (en) | 2019-10-17 |
| US20110217711A1 (en) | 2011-09-08 |
| US11866771B2 (en) | 2024-01-09 |
| JP6188844B2 (ja) | 2017-08-30 |
| US20210363574A1 (en) | 2021-11-25 |
| EP2542660A1 (en) | 2013-01-09 |
| CA2996219C (en) | 2023-08-08 |
| CA2824444A1 (en) | 2011-09-09 |
| JP2016136954A (ja) | 2016-08-04 |
| JP2013520997A (ja) | 2013-06-10 |
| US20170175179A1 (en) | 2017-06-22 |
| EP4464794A2 (en) | 2024-11-20 |
| EP2542660A4 (en) | 2013-08-28 |
| US20110217712A1 (en) | 2011-09-08 |
| CA2824444C (en) | 2018-04-10 |
| EP4464794A3 (en) | 2025-02-19 |
| US9598725B2 (en) | 2017-03-21 |
| US10378048B2 (en) | 2019-08-13 |
| EP3636741A1 (en) | 2020-04-15 |
| WO2011109546A1 (en) | 2011-09-09 |
| US20240093280A1 (en) | 2024-03-21 |
| US8399198B2 (en) | 2013-03-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US11866771B2 (en) | Emulsion chemistry for encapsulated droplets | |
| US8709762B2 (en) | System for hot-start amplification via a multiple emulsion | |
| US9427737B2 (en) | Methods and compositions for using oils for analysis and detection of molecules | |
| US11649487B2 (en) | Nucleic acid amplification | |
| EP3181704B1 (en) | Quantitating high titer samples by digital pcr | |
| US20240102086A1 (en) | UNIVERSAL FLUORESCENT PROBES ACTIVATED WITH RNaseH2 | |
| WO2019077114A1 (en) | EMULSIONS HAVING IMPROVED STABILITY | |
| US20230045126A1 (en) | Devices and methods for determining nucleic acids using digital droplet pcr and related techniques | |
| EP4136258B1 (en) | Method for detecting genetic events | |
| CN110914447A (zh) | 包括水性样品稳定化的测定执行系统 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request |
Effective date: 20230606 |
|
| P11 | Amendment of application requested |
Free format text: ST27 STATUS EVENT CODE: A-2-2-P10-P11-P100 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: AMENDMENT RECEIVED - RESPONSE TO EXAMINER'S REQUISITION Effective date: 20241017 |
|
| W00 | Other event occurred |
Free format text: ST27 STATUS EVENT CODE: A-2-2-W10-W00-W111 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: CORRESPONDENT DETERMINED COMPLIANT Effective date: 20241031 |
|
| P11 | Amendment of application requested |
Free format text: ST27 STATUS EVENT CODE: A-2-2-P10-P11-P102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: AMENDMENT DETERMINED COMPLIANT Effective date: 20250125 |
|
| P13 | Application amended |
Free format text: ST27 STATUS EVENT CODE: A-2-2-P10-P13-X000 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: APPLICATION AMENDED Effective date: 20250125 |
|
| MFA | Maintenance fee for application paid |
Free format text: FEE DESCRIPTION TEXT: MF (APPLICATION, 14TH ANNIV.) - STANDARD Year of fee payment: 14 |
|
| U00 | Fee paid |
Free format text: ST27 STATUS EVENT CODE: A-2-2-U10-U00-U101 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE REQUEST RECEIVED Effective date: 20250218 |
|
| U11 | Full renewal or maintenance fee paid |
Free format text: ST27 STATUS EVENT CODE: A-2-2-U10-U11-U102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE FEE PAYMENT DETERMINED COMPLIANT Effective date: 20250218 Free format text: ST27 STATUS EVENT CODE: A-2-2-U10-U11-U102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE FEE PAYMENT PAID IN FULL Effective date: 20250218 |
|
| D22 | Grant of ip right intended |
Free format text: ST27 STATUS EVENT CODE: A-2-2-D10-D22-D128 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: ALLOWANCE REQUIREMENTS DETERMINED COMPLIANT Effective date: 20251021 |
|
| W00 | Other event occurred |
Free format text: ST27 STATUS EVENT CODE: A-2-2-W10-W00-W100 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: LETTER SENT Effective date: 20251022 |
|
| D00 | Search and/or examination requested or commenced |
Free format text: ST27 STATUS EVENT CODE: A-2-2-D10-D00-D164 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: RESPONSE TO NOTICE OF ALLOWANCE Effective date: 20260219 |
|
| W00 | Other event occurred |
Free format text: ST27 STATUS EVENT CODE: A-2-2-W10-W00-W100 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: LETTER SENT Effective date: 20260413 |
|
| D22 | Grant of ip right intended |
Free format text: ST27 STATUS EVENT CODE: A-2-4-D10-D22-D143 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: PRE-GRANT Effective date: 20260428 |
|
| W00 | Other event occurred |
Free format text: ST27 STATUS EVENT CODE: A-2-2-W10-W00-W111 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: CORRESPONDENT DETERMINED COMPLIANT Effective date: 20260428 |
|
| MFA | Maintenance fee for application paid |
Free format text: FEE DESCRIPTION TEXT: MF (APPLICATION, 15TH ANNIV.) - STANDARD Year of fee payment: 15 |
|
| U00 | Fee paid |
Free format text: ST27 STATUS EVENT CODE: A-4-4-U10-U00-U101 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE REQUEST RECEIVED Effective date: 20260630 |
|
| U11 | Full renewal or maintenance fee paid |
Free format text: ST27 STATUS EVENT CODE: A-4-4-U10-U11-U102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE FEE PAYMENT PAID IN FULL Effective date: 20260630 |