CA3111953C - Method for the generation of compact tale-nucleases and uses thereof - Google Patents
Method for the generation of compact tale-nucleases and uses thereof Download PDFInfo
- Publication number
- CA3111953C CA3111953C CA3111953A CA3111953A CA3111953C CA 3111953 C CA3111953 C CA 3111953C CA 3111953 A CA3111953 A CA 3111953A CA 3111953 A CA3111953 A CA 3111953A CA 3111953 C CA3111953 C CA 3111953C
- Authority
- CA
- Canada
- Prior art keywords
- seq
- compact talen
- dna
- catalytic domain
- sequence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000000034 method Methods 0.000 title claims abstract description 198
- 108020004414 DNA Proteins 0.000 claims abstract description 487
- 230000003197 catalytic effect Effects 0.000 claims abstract description 295
- 238000010459 TALEN Methods 0.000 claims abstract description 156
- 108010043645 Transcription Activator-Like Effector Nucleases Proteins 0.000 claims abstract description 153
- 230000004568 DNA-binding Effects 0.000 claims abstract description 87
- 102000053602 DNA Human genes 0.000 claims abstract description 77
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 53
- 230000008569 process Effects 0.000 claims abstract description 47
- 102000004196 processed proteins & peptides Human genes 0.000 claims abstract description 38
- 229920001184 polypeptide Polymers 0.000 claims abstract description 36
- 238000006471 dimerization reaction Methods 0.000 claims abstract description 16
- 230000000694 effects Effects 0.000 claims description 410
- 108090000623 proteins and genes Proteins 0.000 claims description 280
- 102000004169 proteins and genes Human genes 0.000 claims description 230
- 210000004027 cell Anatomy 0.000 claims description 142
- 238000012545 processing Methods 0.000 claims description 131
- 238000003776 cleavage reaction Methods 0.000 claims description 117
- 210000004899 c-terminal region Anatomy 0.000 claims description 109
- 230000007017 scission Effects 0.000 claims description 101
- 239000000178 monomer Substances 0.000 claims description 55
- 101710163270 Nuclease Proteins 0.000 claims description 54
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 50
- 125000003275 alpha amino acid group Chemical group 0.000 claims description 47
- 210000004962 mammalian cell Anatomy 0.000 claims description 25
- 108010016626 Dipeptides Proteins 0.000 claims description 23
- 230000008685 targeting Effects 0.000 claims description 18
- 102000040430 polynucleotide Human genes 0.000 claims description 16
- 108091033319 polynucleotide Proteins 0.000 claims description 16
- 239000002157 polynucleotide Substances 0.000 claims description 16
- 241000282414 Homo sapiens Species 0.000 claims description 14
- 108700019146 Transgenes Proteins 0.000 claims description 9
- 210000001744 T-lymphocyte Anatomy 0.000 claims description 7
- 206010028980 Neoplasm Diseases 0.000 claims description 4
- 208000035473 Communicable disease Diseases 0.000 claims description 3
- 239000003814 drug Substances 0.000 claims description 3
- 230000036210 malignancy Effects 0.000 claims description 3
- 239000008194 pharmaceutical composition Substances 0.000 claims description 2
- 108700042075 T-Cell Receptor Genes Proteins 0.000 claims 2
- 239000003085 diluting agent Substances 0.000 claims 1
- 229940124597 therapeutic agent Drugs 0.000 claims 1
- 239000013598 vector Substances 0.000 abstract description 75
- 239000000203 mixture Substances 0.000 abstract description 14
- 108010073062 Transcription Activator-Like Effectors Proteins 0.000 description 332
- 108010042407 Endonucleases Proteins 0.000 description 201
- 235000018102 proteins Nutrition 0.000 description 182
- 239000003623 enhancer Substances 0.000 description 155
- 102100031780 Endonuclease Human genes 0.000 description 141
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 136
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 134
- 239000013612 plasmid Substances 0.000 description 100
- 230000005782 double-strand break Effects 0.000 description 77
- 238000003556 assay Methods 0.000 description 71
- 230000000670 limiting effect Effects 0.000 description 70
- 238000005520 cutting process Methods 0.000 description 66
- 230000006780 non-homologous end joining Effects 0.000 description 65
- 230000004927 fusion Effects 0.000 description 63
- 102000004533 Endonucleases Human genes 0.000 description 60
- 108020001778 catalytic domains Proteins 0.000 description 52
- 108020001580 protein domains Proteins 0.000 description 49
- 230000002255 enzymatic effect Effects 0.000 description 47
- 150000007523 nucleic acids Chemical class 0.000 description 44
- 108010008532 Deoxyribonuclease I Proteins 0.000 description 43
- 102000007260 Deoxyribonuclease I Human genes 0.000 description 43
- 108010041758 cleavase Proteins 0.000 description 43
- 230000008439 repair process Effects 0.000 description 43
- 102000039446 nucleic acids Human genes 0.000 description 41
- 108020004707 nucleic acids Proteins 0.000 description 41
- 239000002773 nucleotide Substances 0.000 description 37
- 108091008146 restriction endonucleases Proteins 0.000 description 37
- 230000027455 binding Effects 0.000 description 35
- 125000003729 nucleotide group Chemical group 0.000 description 35
- 230000007018 DNA scission Effects 0.000 description 32
- 108010059724 Micrococcal Nuclease Proteins 0.000 description 32
- 230000037361 pathway Effects 0.000 description 31
- 125000006850 spacer group Chemical group 0.000 description 31
- 238000002744 homologous recombination Methods 0.000 description 30
- 230000006801 homologous recombination Effects 0.000 description 30
- 235000001014 amino acid Nutrition 0.000 description 29
- 241000196324 Embryophyta Species 0.000 description 27
- 229940024606 amino acid Drugs 0.000 description 27
- 150000001413 amino acids Chemical group 0.000 description 27
- 230000006870 function Effects 0.000 description 26
- 230000014509 gene expression Effects 0.000 description 25
- 108010017070 Zinc Finger Nucleases Proteins 0.000 description 24
- 230000006798 recombination Effects 0.000 description 24
- 238000005215 recombination Methods 0.000 description 24
- 108020001507 fusion proteins Proteins 0.000 description 23
- 102000037865 fusion proteins Human genes 0.000 description 23
- 238000010367 cloning Methods 0.000 description 22
- 238000001727 in vivo Methods 0.000 description 22
- 238000002271 resection Methods 0.000 description 22
- 230000007062 hydrolysis Effects 0.000 description 21
- 238000006460 hydrolysis reaction Methods 0.000 description 21
- 238000002703 mutagenesis Methods 0.000 description 21
- 231100000350 mutagenesis Toxicity 0.000 description 21
- 102000004190 Enzymes Human genes 0.000 description 20
- 108090000790 Enzymes Proteins 0.000 description 20
- 101000927313 Homo sapiens DNA replication ATP-dependent helicase DNA2 Proteins 0.000 description 20
- 238000013461 design Methods 0.000 description 19
- 230000009977 dual effect Effects 0.000 description 19
- 238000010363 gene targeting Methods 0.000 description 19
- 238000003780 insertion Methods 0.000 description 18
- 238000013459 approach Methods 0.000 description 17
- 230000037431 insertion Effects 0.000 description 17
- 238000011160 research Methods 0.000 description 17
- 230000009466 transformation Effects 0.000 description 16
- 102000003960 Ligases Human genes 0.000 description 15
- 108090000364 Ligases Proteins 0.000 description 15
- 102100024855 Three-prime repair exonuclease 1 Human genes 0.000 description 15
- 125000000539 amino acid group Chemical group 0.000 description 15
- 230000008859 change Effects 0.000 description 15
- 239000013613 expression plasmid Substances 0.000 description 15
- 238000001415 gene therapy Methods 0.000 description 15
- 239000000126 substance Substances 0.000 description 14
- 108010073254 Colicins Proteins 0.000 description 13
- 241000700159 Rattus Species 0.000 description 13
- 230000037430 deletion Effects 0.000 description 13
- 238000012217 deletion Methods 0.000 description 13
- 230000029087 digestion Effects 0.000 description 13
- 102100033072 DNA replication ATP-dependent helicase DNA2 Human genes 0.000 description 12
- 241000588724 Escherichia coli Species 0.000 description 12
- 108060002716 Exonuclease Proteins 0.000 description 12
- 102100034343 Integrase Human genes 0.000 description 12
- 102000005877 Peptide Initiation Factors Human genes 0.000 description 12
- 108010044843 Peptide Initiation Factors Proteins 0.000 description 12
- 101100108953 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) ANY1 gene Proteins 0.000 description 12
- 102100027172 Transposon Hsmar1 transposase Human genes 0.000 description 12
- 238000000137 annealing Methods 0.000 description 12
- 102000013165 exonuclease Human genes 0.000 description 12
- 230000035772 mutation Effects 0.000 description 12
- 238000001890 transfection Methods 0.000 description 12
- 108091026890 Coding region Proteins 0.000 description 11
- 101000830956 Homo sapiens Three-prime repair exonuclease 1 Proteins 0.000 description 11
- 108010061833 Integrases Proteins 0.000 description 11
- 101150101095 Mmp12 gene Proteins 0.000 description 11
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 11
- 230000001965 increasing effect Effects 0.000 description 11
- 108010036169 three prime repair exonuclease 1 Proteins 0.000 description 11
- 229910052725 zinc Inorganic materials 0.000 description 11
- 239000011701 zinc Substances 0.000 description 11
- 108091033380 Coding strand Proteins 0.000 description 10
- 102100035406 Cysteine desulfurase, mitochondrial Human genes 0.000 description 10
- 102100027828 DNA repair protein XRCC4 Human genes 0.000 description 10
- 101001023837 Homo sapiens Cysteine desulfurase, mitochondrial Proteins 0.000 description 10
- 101000649315 Homo sapiens DNA repair protein XRCC4 Proteins 0.000 description 10
- 101000830950 Homo sapiens Three prime repair exonuclease 2 Proteins 0.000 description 10
- 101000836339 Homo sapiens Transposon Hsmar1 transposase Proteins 0.000 description 10
- 102000004160 Phosphoric Monoester Hydrolases Human genes 0.000 description 10
- 108090000608 Phosphoric Monoester Hydrolases Proteins 0.000 description 10
- 108091000080 Phosphotransferase Proteins 0.000 description 10
- 101710183280 Topoisomerase Proteins 0.000 description 10
- 102000008579 Transposases Human genes 0.000 description 10
- 108010020764 Transposases Proteins 0.000 description 10
- 239000013078 crystal Substances 0.000 description 10
- 238000000338 in vitro Methods 0.000 description 10
- 102000020233 phosphotransferase Human genes 0.000 description 10
- 101100328487 Arabidopsis thaliana NFS2 gene Proteins 0.000 description 9
- 101001137540 Bos taurus Endonuclease G, mitochondrial Proteins 0.000 description 9
- 101710159064 Colicin-E7 Proteins 0.000 description 9
- 101001137538 Homo sapiens Endonuclease G, mitochondrial Proteins 0.000 description 9
- 230000001419 dependent effect Effects 0.000 description 9
- 230000002068 genetic effect Effects 0.000 description 9
- 150000004000 hexols Chemical class 0.000 description 9
- 239000011159 matrix material Substances 0.000 description 9
- 239000002243 precursor Substances 0.000 description 9
- 210000001519 tissue Anatomy 0.000 description 9
- 101100370338 Bos taurus TREX1 gene Proteins 0.000 description 8
- 230000033616 DNA repair Effects 0.000 description 8
- 101710204468 Endonuclease YncB Proteins 0.000 description 8
- 108010068250 Herpes Simplex Virus Protein Vmw65 Proteins 0.000 description 8
- 101100370340 Mus musculus Trex1 gene Proteins 0.000 description 8
- 241000700605 Viruses Species 0.000 description 8
- 238000006243 chemical reaction Methods 0.000 description 8
- 238000002474 experimental method Methods 0.000 description 8
- 102000045960 human DNA2 Human genes 0.000 description 8
- 230000004048 modification Effects 0.000 description 8
- 238000012986 modification Methods 0.000 description 8
- 239000000758 substrate Substances 0.000 description 8
- 229910009891 LiAc Inorganic materials 0.000 description 7
- 241000699666 Mus <mouse, genus> Species 0.000 description 7
- 239000003795 chemical substances by application Substances 0.000 description 7
- 239000012634 fragment Substances 0.000 description 7
- 238000010362 genome editing Methods 0.000 description 7
- 102000054586 human TREX2 Human genes 0.000 description 7
- 230000002779 inactivation Effects 0.000 description 7
- 230000014759 maintenance of location Effects 0.000 description 7
- 210000004897 n-terminal region Anatomy 0.000 description 7
- 239000013642 negative control Substances 0.000 description 7
- 239000000047 product Substances 0.000 description 7
- 230000009261 transgenic effect Effects 0.000 description 7
- 101000876382 Enterobacteria phage T3 Endodeoxyribonuclease 1 Proteins 0.000 description 6
- 108091092195 Intron Proteins 0.000 description 6
- 241000205160 Pyrococcus Species 0.000 description 6
- 238000006555 catalytic reaction Methods 0.000 description 6
- 230000002759 chromosomal effect Effects 0.000 description 6
- 210000000349 chromosome Anatomy 0.000 description 6
- 238000012937 correction Methods 0.000 description 6
- 230000002708 enhancing effect Effects 0.000 description 6
- 239000013604 expression vector Substances 0.000 description 6
- 230000001939 inductive effect Effects 0.000 description 6
- 108091006086 inhibitor proteins Proteins 0.000 description 6
- 230000007246 mechanism Effects 0.000 description 6
- 230000001105 regulatory effect Effects 0.000 description 6
- 230000001225 therapeutic effect Effects 0.000 description 6
- 238000013518 transcription Methods 0.000 description 6
- 230000035897 transcription Effects 0.000 description 6
- 210000005253 yeast cell Anatomy 0.000 description 6
- 108010007577 Exodeoxyribonuclease I Proteins 0.000 description 5
- 102100029075 Exonuclease 1 Human genes 0.000 description 5
- 241000187479 Mycobacterium tuberculosis Species 0.000 description 5
- 108091034117 Oligonucleotide Proteins 0.000 description 5
- 108091008874 T cell receptors Proteins 0.000 description 5
- 102000016266 T-Cell Antigen Receptors Human genes 0.000 description 5
- 230000022131 cell cycle Effects 0.000 description 5
- 239000003153 chemical reaction reagent Substances 0.000 description 5
- 238000010276 construction Methods 0.000 description 5
- 210000003527 eukaryotic cell Anatomy 0.000 description 5
- 239000000499 gel Substances 0.000 description 5
- 230000003993 interaction Effects 0.000 description 5
- 238000005304 joining Methods 0.000 description 5
- 239000000463 material Substances 0.000 description 5
- 230000001404 mediated effect Effects 0.000 description 5
- 239000006187 pill Substances 0.000 description 5
- 229920001223 polyethylene glycol Polymers 0.000 description 5
- 210000001938 protoplast Anatomy 0.000 description 5
- 238000012552 review Methods 0.000 description 5
- 238000006467 substitution reaction Methods 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- 238000012546 transfer Methods 0.000 description 5
- 238000013519 translation Methods 0.000 description 5
- 230000014621 translational initiation Effects 0.000 description 5
- 230000008265 DNA repair mechanism Effects 0.000 description 4
- 101000763322 Homo sapiens M1-specific T cell receptor beta chain Proteins 0.000 description 4
- 101000763321 Homo sapiens T cell receptor beta chain MC.7.G5 Proteins 0.000 description 4
- 101150059802 KU80 gene Proteins 0.000 description 4
- CXOFVDLJLONNDW-UHFFFAOYSA-N Phenytoin Chemical compound N1C(=O)NC(=O)C1(C=1C=CC=CC=1)C1=CC=CC=C1 CXOFVDLJLONNDW-UHFFFAOYSA-N 0.000 description 4
- 241000589516 Pseudomonas Species 0.000 description 4
- 102100024872 Three prime repair exonuclease 2 Human genes 0.000 description 4
- 102100036973 X-ray repair cross-complementing protein 5 Human genes 0.000 description 4
- 230000004913 activation Effects 0.000 description 4
- 210000004102 animal cell Anatomy 0.000 description 4
- 230000033228 biological regulation Effects 0.000 description 4
- 238000012512 characterization method Methods 0.000 description 4
- 238000004891 communication Methods 0.000 description 4
- 239000012636 effector Substances 0.000 description 4
- 108010050663 endodeoxyribonuclease CreI Proteins 0.000 description 4
- 230000009545 invasion Effects 0.000 description 4
- 229920001451 polypropylene glycol Polymers 0.000 description 4
- 238000000746 purification Methods 0.000 description 4
- 230000008707 rearrangement Effects 0.000 description 4
- 230000010076 replication Effects 0.000 description 4
- 239000013603 viral vector Substances 0.000 description 4
- 230000003612 virological effect Effects 0.000 description 4
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 3
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 3
- 102000008102 Ankyrins Human genes 0.000 description 3
- 108010049777 Ankyrins Proteins 0.000 description 3
- 101150076489 B gene Proteins 0.000 description 3
- 108050008316 DNA endonuclease RBBP8 Proteins 0.000 description 3
- 102100039524 DNA endonuclease RBBP8 Human genes 0.000 description 3
- 241000702421 Dependoparvovirus Species 0.000 description 3
- 241000193385 Geobacillus stearothermophilus Species 0.000 description 3
- 102000004157 Hydrolases Human genes 0.000 description 3
- 108090000604 Hydrolases Proteins 0.000 description 3
- 108010091358 Hypoxanthine Phosphoribosyltransferase Proteins 0.000 description 3
- 101000836313 Moraxella sp Type II restriction enzyme MspI Proteins 0.000 description 3
- 241000193390 Parageobacillus thermoglucosidasius Species 0.000 description 3
- 102000044126 RNA-Binding Proteins Human genes 0.000 description 3
- 108700008625 Reporter Genes Proteins 0.000 description 3
- 108020004682 Single-Stranded DNA Proteins 0.000 description 3
- 102000004357 Transferases Human genes 0.000 description 3
- 108090000992 Transferases Proteins 0.000 description 3
- 240000008042 Zea mays Species 0.000 description 3
- 101710185494 Zinc finger protein Proteins 0.000 description 3
- 102100023597 Zinc finger protein 816 Human genes 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 3
- 230000001580 bacterial effect Effects 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 3
- 239000000969 carrier Substances 0.000 description 3
- 238000000423 cell based assay Methods 0.000 description 3
- 230000001413 cellular effect Effects 0.000 description 3
- 210000004978 chinese hamster ovary cell Anatomy 0.000 description 3
- 238000012411 cloning technique Methods 0.000 description 3
- 230000009918 complex formation Effects 0.000 description 3
- 230000008878 coupling Effects 0.000 description 3
- 238000010168 coupling process Methods 0.000 description 3
- 238000005859 coupling reaction Methods 0.000 description 3
- 230000012361 double-strand break repair Effects 0.000 description 3
- 238000000684 flow cytometry Methods 0.000 description 3
- 238000003209 gene knockout Methods 0.000 description 3
- RAXXELZNTBOGNW-UHFFFAOYSA-N imidazole Natural products C1=CNC=N1 RAXXELZNTBOGNW-UHFFFAOYSA-N 0.000 description 3
- 238000011065 in-situ storage Methods 0.000 description 3
- 239000003112 inhibitor Substances 0.000 description 3
- 230000017730 intein-mediated protein splicing Effects 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 229910052751 metal Inorganic materials 0.000 description 3
- 239000002184 metal Substances 0.000 description 3
- 238000010369 molecular cloning Methods 0.000 description 3
- 101150071637 mre11 gene Proteins 0.000 description 3
- 238000012216 screening Methods 0.000 description 3
- 238000000926 separation method Methods 0.000 description 3
- 230000005783 single-strand break Effects 0.000 description 3
- 241000894007 species Species 0.000 description 3
- 238000001228 spectrum Methods 0.000 description 3
- 238000010561 standard procedure Methods 0.000 description 3
- 230000000638 stimulation Effects 0.000 description 3
- 238000001262 western blot Methods 0.000 description 3
- 101710159080 Aconitate hydratase A Proteins 0.000 description 2
- 101710159078 Aconitate hydratase B Proteins 0.000 description 2
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 2
- 235000005340 Asparagus officinalis Nutrition 0.000 description 2
- 108010004586 Ataxia Telangiectasia Mutated Proteins Proteins 0.000 description 2
- 241000194103 Bacillus pumilus Species 0.000 description 2
- 241000194110 Bacillus sp. (in: Bacteria) Species 0.000 description 2
- 241000193764 Brevibacillus brevis Species 0.000 description 2
- 235000002566 Capsicum Nutrition 0.000 description 2
- 240000008574 Capsicum frutescens Species 0.000 description 2
- 108010019670 Chimeric Antigen Receptors Proteins 0.000 description 2
- 108010077544 Chromatin Proteins 0.000 description 2
- 208000037088 Chromosome Breakage Diseases 0.000 description 2
- 108020004638 Circular DNA Proteins 0.000 description 2
- 108091028075 Circular RNA Proteins 0.000 description 2
- 241000252212 Danio rerio Species 0.000 description 2
- 101710134695 Endodeoxyribonuclease 1 Proteins 0.000 description 2
- 101710180995 Endonuclease 1 Proteins 0.000 description 2
- 241000588722 Escherichia Species 0.000 description 2
- 241000233866 Fungi Species 0.000 description 2
- 108700035841 GIY-YIG endonucleases Proteins 0.000 description 2
- 102000052907 GIY-YIG endonucleases Human genes 0.000 description 2
- 208000031448 Genomic Instability Diseases 0.000 description 2
- 108091027874 Group I catalytic intron Proteins 0.000 description 2
- 108050008753 HNH endonucleases Proteins 0.000 description 2
- 102000000310 HNH endonucleases Human genes 0.000 description 2
- 102000006947 Histones Human genes 0.000 description 2
- 108010033040 Histones Proteins 0.000 description 2
- 101001008919 Homo sapiens Kallikrein-10 Proteins 0.000 description 2
- 102100029098 Hypoxanthine-guanine phosphoribosyltransferase Human genes 0.000 description 2
- 208000026350 Inborn Genetic disease Diseases 0.000 description 2
- 102100027613 Kallikrein-10 Human genes 0.000 description 2
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 2
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 2
- 241000208822 Lactuca Species 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 241000699670 Mus sp. Species 0.000 description 2
- 108010021466 Mutant Proteins Proteins 0.000 description 2
- 102000008300 Mutant Proteins Human genes 0.000 description 2
- 101150081841 NBN gene Proteins 0.000 description 2
- 241000208125 Nicotiana Species 0.000 description 2
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 2
- 244000061176 Nicotiana tabacum Species 0.000 description 2
- 108090001050 Phosphoric Diester Hydrolases Proteins 0.000 description 2
- 102000004861 Phosphoric Diester Hydrolases Human genes 0.000 description 2
- 101000658568 Planomicrobium okeanokoites Type II restriction enzyme FokI Proteins 0.000 description 2
- 108010072866 Prostate-Specific Antigen Proteins 0.000 description 2
- 102100038358 Prostate-specific antigen Human genes 0.000 description 2
- 241000508269 Psidium Species 0.000 description 2
- 101710105008 RNA-binding protein Proteins 0.000 description 2
- 241000700157 Rattus norvegicus Species 0.000 description 2
- 101100029566 Rattus norvegicus Rabggta gene Proteins 0.000 description 2
- 108020004511 Recombinant DNA Proteins 0.000 description 2
- 101001025539 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) Homothallic switching endonuclease Proteins 0.000 description 2
- 241000700584 Simplexvirus Species 0.000 description 2
- 240000003768 Solanum lycopersicum Species 0.000 description 2
- 235000002560 Solanum lycopersicum Nutrition 0.000 description 2
- 241000543700 Staphylococcus virus Twort Species 0.000 description 2
- 241000193998 Streptococcus pneumoniae Species 0.000 description 2
- 208000012827 T-B+ severe combined immunodeficiency due to gamma chain deficiency Diseases 0.000 description 2
- 208000023940 X-Linked Combined Immunodeficiency disease Diseases 0.000 description 2
- 201000007146 X-linked severe combined immunodeficiency Diseases 0.000 description 2
- 241000589634 Xanthomonas Species 0.000 description 2
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 2
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 2
- PTFCDOFLOPIGGS-UHFFFAOYSA-N Zinc dication Chemical compound [Zn+2] PTFCDOFLOPIGGS-UHFFFAOYSA-N 0.000 description 2
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 2
- 239000012190 activator Substances 0.000 description 2
- 235000004279 alanine Nutrition 0.000 description 2
- 229960003767 alanine Drugs 0.000 description 2
- 125000000637 arginyl group Chemical group N[C@@H](CCCNC(N)=N)C(=O)* 0.000 description 2
- 210000001106 artificial yeast chromosome Anatomy 0.000 description 2
- CKLJMWTZIZZHCS-REOHCLBHSA-N aspartic acid group Chemical group N[C@@H](CC(=O)O)C(=O)O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 239000001390 capsicum minimum Substances 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
- 238000004113 cell culture Methods 0.000 description 2
- 239000007795 chemical reaction product Substances 0.000 description 2
- 210000003763 chloroplast Anatomy 0.000 description 2
- 210000003483 chromatin Anatomy 0.000 description 2
- 230000002301 combined effect Effects 0.000 description 2
- 230000001276 controlling effect Effects 0.000 description 2
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 230000007812 deficiency Effects 0.000 description 2
- 230000002950 deficient Effects 0.000 description 2
- 230000002939 deleterious effect Effects 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 239000000539 dimer Substances 0.000 description 2
- 230000034431 double-strand break repair via homologous recombination Effects 0.000 description 2
- 241001493065 dsRNA viruses Species 0.000 description 2
- 238000001962 electrophoresis Methods 0.000 description 2
- 238000004520 electroporation Methods 0.000 description 2
- 230000008030 elimination Effects 0.000 description 2
- 238000003379 elimination reaction Methods 0.000 description 2
- 108010002082 endometriosis protein-1 Proteins 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000001973 epigenetic effect Effects 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 101150014310 fem-3 gene Proteins 0.000 description 2
- 238000001476 gene delivery Methods 0.000 description 2
- 238000003197 gene knockdown Methods 0.000 description 2
- 208000016361 genetic disease Diseases 0.000 description 2
- 238000009650 gentamicin protection assay Methods 0.000 description 2
- 125000000404 glutamine group Chemical group N[C@@H](CCC(N)=O)C(=O)* 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical compound O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 description 2
- 239000000833 heterodimer Substances 0.000 description 2
- 239000000710 homodimer Substances 0.000 description 2
- 230000008105 immune reaction Effects 0.000 description 2
- 230000002163 immunogen Effects 0.000 description 2
- 238000000099 in vitro assay Methods 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 230000006698 induction Effects 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 230000010354 integration Effects 0.000 description 2
- 238000002955 isolation Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 235000009973 maize Nutrition 0.000 description 2
- 210000000415 mammalian chromosome Anatomy 0.000 description 2
- 210000001161 mammalian embryo Anatomy 0.000 description 2
- 229930182817 methionine Natural products 0.000 description 2
- 210000003470 mitochondria Anatomy 0.000 description 2
- 230000000869 mutational effect Effects 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- 238000005457 optimization Methods 0.000 description 2
- 210000003463 organelle Anatomy 0.000 description 2
- 230000002018 overexpression Effects 0.000 description 2
- 238000004806 packaging method and process Methods 0.000 description 2
- 244000000003 plant pathogen Species 0.000 description 2
- 239000013641 positive control Substances 0.000 description 2
- 230000012743 protein tagging Effects 0.000 description 2
- 101150010682 rad50 gene Proteins 0.000 description 2
- 238000002708 random mutagenesis Methods 0.000 description 2
- 230000002829 reductive effect Effects 0.000 description 2
- 238000010839 reverse transcription Methods 0.000 description 2
- 238000010186 staining Methods 0.000 description 2
- 229940031000 streptococcus pneumoniae Drugs 0.000 description 2
- RWQNBRDOKXIBIV-UHFFFAOYSA-N thymine Chemical group CC1=CNC(=O)NC1=O RWQNBRDOKXIBIV-UHFFFAOYSA-N 0.000 description 2
- 231100000419 toxicity Toxicity 0.000 description 2
- 230000001988 toxicity Effects 0.000 description 2
- 241000701161 unidentified adenovirus Species 0.000 description 2
- 241001430294 unidentified retrovirus Species 0.000 description 2
- BSDCIRGNJKZPFV-GWOFURMSSA-N (2r,3s,4r,5r)-2-(hydroxymethyl)-5-(2,5,6-trichlorobenzimidazol-1-yl)oxolane-3,4-diol Chemical compound O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1C2=CC(Cl)=C(Cl)C=C2N=C1Cl BSDCIRGNJKZPFV-GWOFURMSSA-N 0.000 description 1
- XSYUPRQVAHJETO-WPMUBMLPSA-N (2s)-2-[[(2s)-2-[[(2s)-2-[[(2s)-2-[[(2s)-2-[[(2s)-2-amino-3-(1h-imidazol-5-yl)propanoyl]amino]-3-(1h-imidazol-5-yl)propanoyl]amino]-3-(1h-imidazol-5-yl)propanoyl]amino]-3-(1h-imidazol-5-yl)propanoyl]amino]-3-(1h-imidazol-5-yl)propanoyl]amino]-3-(1h-imidaz Chemical compound C([C@H](N)C(=O)N[C@@H](CC=1NC=NC=1)C(=O)N[C@@H](CC=1NC=NC=1)C(=O)N[C@@H](CC=1NC=NC=1)C(=O)N[C@@H](CC=1NC=NC=1)C(=O)N[C@@H](CC=1NC=NC=1)C(O)=O)C1=CN=CN1 XSYUPRQVAHJETO-WPMUBMLPSA-N 0.000 description 1
- BZSALXKCVOJCJJ-IPEMHBBOSA-N (4s)-4-[[(2s)-2-acetamido-3-methylbutanoyl]amino]-5-[[(2s)-1-[[(2s)-1-[[(2s,3r)-1-[[(2s)-1-[[(2s)-1-[[2-[[(2s)-1-amino-1-oxo-3-phenylpropan-2-yl]amino]-2-oxoethyl]amino]-5-(diaminomethylideneamino)-1-oxopentan-2-yl]amino]-1-oxopropan-2-yl]amino]-3-hydroxy Chemical compound CC(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCCC)C(=O)N[C@@H](CCCC)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCN=C(N)N)C(=O)NCC(=O)N[C@H](C(N)=O)CC1=CC=CC=C1 BZSALXKCVOJCJJ-IPEMHBBOSA-N 0.000 description 1
- KIAPWMKFHIKQOZ-UHFFFAOYSA-N 2-[[(4-fluorophenyl)-oxomethyl]amino]benzoic acid methyl ester Chemical compound COC(=O)C1=CC=CC=C1NC(=O)C1=CC=C(F)C=C1 KIAPWMKFHIKQOZ-UHFFFAOYSA-N 0.000 description 1
- 101710169336 5'-deoxyadenosine deaminase Proteins 0.000 description 1
- 239000013607 AAV vector Substances 0.000 description 1
- 101150005709 ARG4 gene Proteins 0.000 description 1
- 241001019659 Acremonium <Plectosphaerellaceae> Species 0.000 description 1
- 241000228431 Acremonium chrysogenum Species 0.000 description 1
- 241000251468 Actinopterygii Species 0.000 description 1
- 229930024421 Adenine Natural products 0.000 description 1
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 1
- 102100036664 Adenosine deaminase Human genes 0.000 description 1
- 108700028369 Alleles Proteins 0.000 description 1
- 241000710929 Alphavirus Species 0.000 description 1
- 241000192542 Anabaena Species 0.000 description 1
- 241000192531 Anabaena sp. Species 0.000 description 1
- 241000219194 Arabidopsis Species 0.000 description 1
- DCXYFEDJOCDNAF-UHFFFAOYSA-N Asparagine Natural products OC(=O)C(N)CC(N)=O DCXYFEDJOCDNAF-UHFFFAOYSA-N 0.000 description 1
- 244000003416 Asparagus officinalis Species 0.000 description 1
- 241000228212 Aspergillus Species 0.000 description 1
- 241000351920 Aspergillus nidulans Species 0.000 description 1
- 241000228245 Aspergillus niger Species 0.000 description 1
- 240000006439 Aspergillus oryzae Species 0.000 description 1
- 235000002247 Aspergillus oryzae Nutrition 0.000 description 1
- 241001465318 Aspergillus terreus Species 0.000 description 1
- 102000002804 Ataxia Telangiectasia Mutated Proteins Human genes 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 241000193747 Bacillus firmus Species 0.000 description 1
- 241000194107 Bacillus megaterium Species 0.000 description 1
- 241001249117 Bacillus mojavensis Species 0.000 description 1
- 241000579749 Bacillus phage Bastille Species 0.000 description 1
- 101100160356 Bacillus subtilis (strain 168) yncB gene Proteins 0.000 description 1
- 241000193388 Bacillus thuringiensis Species 0.000 description 1
- ROFVEXUMMXZLPA-UHFFFAOYSA-N Bipyridyl Chemical compound N1=CC=CC=C1C1=CC=CC=N1 ROFVEXUMMXZLPA-UHFFFAOYSA-N 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- 101100263837 Bovine ephemeral fever virus (strain BB7721) beta gene Proteins 0.000 description 1
- 241000219198 Brassica Species 0.000 description 1
- 235000011331 Brassica Nutrition 0.000 description 1
- 235000011303 Brassica alboglabra Nutrition 0.000 description 1
- 240000002791 Brassica napus Species 0.000 description 1
- 235000011293 Brassica napus Nutrition 0.000 description 1
- 240000007124 Brassica oleracea Species 0.000 description 1
- 235000011302 Brassica oleracea Nutrition 0.000 description 1
- 240000008100 Brassica rapa Species 0.000 description 1
- 235000011292 Brassica rapa Nutrition 0.000 description 1
- 101150029409 CFTR gene Proteins 0.000 description 1
- 101150075799 CPX1 gene Proteins 0.000 description 1
- 241000244202 Caenorhabditis Species 0.000 description 1
- 241000244203 Caenorhabditis elegans Species 0.000 description 1
- 101100268665 Caenorhabditis elegans acc-1 gene Proteins 0.000 description 1
- 241000282465 Canis Species 0.000 description 1
- 241000282461 Canis lupus Species 0.000 description 1
- 102220545952 Cell division cycle 5-like protein_K82A_mutation Human genes 0.000 description 1
- 241000195597 Chlamydomonas reinhardtii Species 0.000 description 1
- 108091060290 Chromatid Proteins 0.000 description 1
- 241001674013 Chrysosporium lucknowense Species 0.000 description 1
- 244000241235 Citrullus lanatus Species 0.000 description 1
- 235000009831 Citrullus lanatus Nutrition 0.000 description 1
- 241000207199 Citrus Species 0.000 description 1
- 244000089742 Citrus aurantifolia Species 0.000 description 1
- 235000008733 Citrus aurantifolia Nutrition 0.000 description 1
- 235000001759 Citrus maxima Nutrition 0.000 description 1
- 244000276331 Citrus maxima Species 0.000 description 1
- 235000001938 Citrus medica Nutrition 0.000 description 1
- 240000004307 Citrus medica Species 0.000 description 1
- 241001672694 Citrus reticulata Species 0.000 description 1
- 102100038445 Claudin-2 Human genes 0.000 description 1
- 101710135385 Colicin-E9 immunity protein Proteins 0.000 description 1
- 241000711573 Coronaviridae Species 0.000 description 1
- 244000241257 Cucumis melo Species 0.000 description 1
- 235000009842 Cucumis melo Nutrition 0.000 description 1
- 235000010071 Cucumis prophetarum Nutrition 0.000 description 1
- 244000024469 Cucumis prophetarum Species 0.000 description 1
- 241000219122 Cucurbita Species 0.000 description 1
- 235000009852 Cucurbita pepo Nutrition 0.000 description 1
- 240000001980 Cucurbita pepo Species 0.000 description 1
- 241000701022 Cytomegalovirus Species 0.000 description 1
- 108010060248 DNA Ligase ATP Proteins 0.000 description 1
- 102000021107 DNA end binding proteins Human genes 0.000 description 1
- 108091011122 DNA end binding proteins Proteins 0.000 description 1
- 101710113391 DNA endonuclease I-CreI Proteins 0.000 description 1
- 230000007064 DNA hydrolysis Effects 0.000 description 1
- 102100033195 DNA ligase 4 Human genes 0.000 description 1
- 230000004543 DNA replication Effects 0.000 description 1
- 241000450599 DNA viruses Species 0.000 description 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
- 101710161637 DNA-entry nuclease Proteins 0.000 description 1
- 206010011878 Deafness Diseases 0.000 description 1
- 241000255581 Drosophila <fruit fly, genus> Species 0.000 description 1
- 241000255601 Drosophila melanogaster Species 0.000 description 1
- 102100021008 Endonuclease G, mitochondrial Human genes 0.000 description 1
- 101100316840 Enterobacteria phage P4 Beta gene Proteins 0.000 description 1
- 101000889905 Enterobacteria phage RB3 Intron-associated endonuclease 3 Proteins 0.000 description 1
- 101000889904 Enterobacteria phage T4 Defective intron-associated endonuclease 3 Proteins 0.000 description 1
- 101000889900 Enterobacteria phage T4 Intron-associated endonuclease 1 Proteins 0.000 description 1
- 101000889899 Enterobacteria phage T4 Intron-associated endonuclease 2 Proteins 0.000 description 1
- 241000283087 Equus Species 0.000 description 1
- 241000283073 Equus caballus Species 0.000 description 1
- 101100329777 Escherichia coli (strain K12) curA gene Proteins 0.000 description 1
- 241001452028 Escherichia coli DH1 Species 0.000 description 1
- 101100383218 Escherichia coli colE7 gene Proteins 0.000 description 1
- 101710185850 Exodeoxyribonuclease Proteins 0.000 description 1
- 101710193865 Exodeoxyribonuclease 1 Proteins 0.000 description 1
- 108700024394 Exon Proteins 0.000 description 1
- 102100031806 Fas-binding factor 1 Human genes 0.000 description 1
- 241000282324 Felis Species 0.000 description 1
- 241000282326 Felis catus Species 0.000 description 1
- 241000710831 Flavivirus Species 0.000 description 1
- 208000000666 Fowlpox Diseases 0.000 description 1
- 230000010337 G2 phase Effects 0.000 description 1
- 235000014820 Galium aparine Nutrition 0.000 description 1
- 240000005702 Galium aparine Species 0.000 description 1
- 241000287826 Gallus Species 0.000 description 1
- 241000287828 Gallus gallus Species 0.000 description 1
- 241000626621 Geobacillus Species 0.000 description 1
- 241000827781 Geobacillus sp. Species 0.000 description 1
- 241000941423 Grom virus Species 0.000 description 1
- 101150009006 HIS3 gene Proteins 0.000 description 1
- 108060003760 HNH nuclease Proteins 0.000 description 1
- 102000029812 HNH nuclease Human genes 0.000 description 1
- 241000606768 Haemophilus influenzae Species 0.000 description 1
- 102000001554 Hemoglobins Human genes 0.000 description 1
- 108010054147 Hemoglobins Proteins 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- MAJYPBAJPNUFPV-BQBZGAKWSA-N His-Cys Chemical compound SC[C@@H](C(O)=O)NC(=O)[C@@H](N)CC1=CN=CN1 MAJYPBAJPNUFPV-BQBZGAKWSA-N 0.000 description 1
- 108091006054 His-tagged proteins Proteins 0.000 description 1
- 108010016918 Histone-Lysine N-Methyltransferase Proteins 0.000 description 1
- 101710153466 Histone-lysine N-methyltransferase SETMAR Proteins 0.000 description 1
- 101000882901 Homo sapiens Claudin-2 Proteins 0.000 description 1
- 101001055227 Homo sapiens Cytokine receptor common subunit gamma Proteins 0.000 description 1
- 101000746134 Homo sapiens DNA endonuclease RBBP8 Proteins 0.000 description 1
- 101000618535 Homo sapiens DNA repair protein complementing XP-C cells Proteins 0.000 description 1
- 101000918264 Homo sapiens Exonuclease 1 Proteins 0.000 description 1
- 101001065295 Homo sapiens Fas-binding factor 1 Proteins 0.000 description 1
- 101001012669 Homo sapiens Melanoma inhibitory activity protein 2 Proteins 0.000 description 1
- 101001128634 Homo sapiens NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial Proteins 0.000 description 1
- 101000687346 Homo sapiens PR domain zinc finger protein 2 Proteins 0.000 description 1
- 241000209219 Hordeum Species 0.000 description 1
- 235000007340 Hordeum vulgare Nutrition 0.000 description 1
- 240000005979 Hordeum vulgare Species 0.000 description 1
- 241000701074 Human alphaherpesvirus 2 Species 0.000 description 1
- 241000701044 Human gammaherpesvirus 4 Species 0.000 description 1
- 241000725303 Human immunodeficiency virus Species 0.000 description 1
- 241000713772 Human immunodeficiency virus 1 Species 0.000 description 1
- 101710116034 Immunity protein Proteins 0.000 description 1
- 102000006496 Immunoglobulin Heavy Chains Human genes 0.000 description 1
- 108010019476 Immunoglobulin Heavy Chains Proteins 0.000 description 1
- 101710169637 Intron-associated endonuclease 1 Proteins 0.000 description 1
- 101710169638 Intron-associated endonuclease 2 Proteins 0.000 description 1
- 101710169639 Intron-associated endonuclease 3 Proteins 0.000 description 1
- 108010025815 Kanamycin Kinase Proteins 0.000 description 1
- 241000235649 Kluyveromyces Species 0.000 description 1
- 241001138401 Kluyveromyces lactis Species 0.000 description 1
- 241000191953 Kocuria varians Species 0.000 description 1
- 241000235058 Komagataella pastoris Species 0.000 description 1
- 102000015335 Ku Autoantigen Human genes 0.000 description 1
- 108010025026 Ku Autoantigen Proteins 0.000 description 1
- DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 description 1
- ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 description 1
- 125000002707 L-tryptophyl group Chemical group [H]C1=C([H])C([H])=C2C(C([C@](N([H])[H])(C(=O)[*])[H])([H])[H])=C([H])N([H])C2=C1[H] 0.000 description 1
- 108091026898 Leader sequence (mRNA) Proteins 0.000 description 1
- 241000713666 Lentivirus Species 0.000 description 1
- 102100026964 M1-specific T cell receptor beta chain Human genes 0.000 description 1
- 108010059343 MM Form Creatine Kinase Proteins 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 201000005505 Measles Diseases 0.000 description 1
- 241000219823 Medicago Species 0.000 description 1
- 235000010624 Medicago sativa Nutrition 0.000 description 1
- 240000004658 Medicago sativa Species 0.000 description 1
- 102100029778 Melanoma inhibitory activity protein 2 Human genes 0.000 description 1
- 241000288145 Meleagris Species 0.000 description 1
- 241000288147 Meleagris gallopavo Species 0.000 description 1
- 102000012750 Membrane Glycoproteins Human genes 0.000 description 1
- 108010090054 Membrane Glycoproteins Proteins 0.000 description 1
- 241000863391 Methylophilus Species 0.000 description 1
- 108060004795 Methyltransferase Proteins 0.000 description 1
- 241001059271 Micrococca Species 0.000 description 1
- 241000191951 Micrococcus lylae Species 0.000 description 1
- 241000235575 Mortierella Species 0.000 description 1
- 241000907999 Mortierella alpina Species 0.000 description 1
- 241000699660 Mus musculus Species 0.000 description 1
- 241000186359 Mycobacterium Species 0.000 description 1
- 108700010674 N-acetylVal-Nle(7,8)- allatotropin (5-13) Proteins 0.000 description 1
- PYUSHNKNPOHWEZ-YFKPBYRVSA-N N-formyl-L-methionine Chemical compound CSCC[C@@H](C(O)=O)NC=O PYUSHNKNPOHWEZ-YFKPBYRVSA-N 0.000 description 1
- 102100032194 NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial Human genes 0.000 description 1
- 241000714209 Norwalk virus Species 0.000 description 1
- 241000192673 Nostoc sp. Species 0.000 description 1
- 101710202393 Nuclease NucM Proteins 0.000 description 1
- 241000277334 Oncorhynchus Species 0.000 description 1
- 241000277275 Oncorhynchus mykiss Species 0.000 description 1
- 241000702244 Orthoreovirus Species 0.000 description 1
- 241000209094 Oryza Species 0.000 description 1
- 240000008346 Oryza glaberrima Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 208000032366 Oversensing Diseases 0.000 description 1
- 102100024885 PR domain zinc finger protein 2 Human genes 0.000 description 1
- 108091081548 Palindromic sequence Proteins 0.000 description 1
- 241001148116 Paucimonas lemoignei Species 0.000 description 1
- 241000228143 Penicillium Species 0.000 description 1
- 241000228150 Penicillium chrysogenum Species 0.000 description 1
- 241000228153 Penicillium citrinum Species 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 241000235648 Pichia Species 0.000 description 1
- 241000709664 Picornaviridae Species 0.000 description 1
- 241000219843 Pisum Species 0.000 description 1
- 235000010582 Pisum sativum Nutrition 0.000 description 1
- 240000004713 Pisum sativum Species 0.000 description 1
- 108700001094 Plant Genes Proteins 0.000 description 1
- 101100271190 Plasmodium falciparum (isolate 3D7) ATAT gene Proteins 0.000 description 1
- 239000004952 Polyamide Substances 0.000 description 1
- 239000002202 Polyethylene glycol Substances 0.000 description 1
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 241000125945 Protoparvovirus Species 0.000 description 1
- 241000589517 Pseudomonas aeruginosa Species 0.000 description 1
- 102000015097 RNA Splicing Factors Human genes 0.000 description 1
- 108010039259 RNA Splicing Factors Proteins 0.000 description 1
- 108700020471 RNA-Binding Proteins Proteins 0.000 description 1
- 230000004570 RNA-binding Effects 0.000 description 1
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 description 1
- 206010037742 Rabies Diseases 0.000 description 1
- 241000711798 Rabies lyssavirus Species 0.000 description 1
- 108020005091 Replication Origin Proteins 0.000 description 1
- 241000712907 Retroviridae Species 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 101100394989 Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009) hisI gene Proteins 0.000 description 1
- 108050008465 Ribonucleoside-diphosphate reductase large subunit Proteins 0.000 description 1
- 230000018199 S phase Effects 0.000 description 1
- 229910003797 SPO1 Inorganic materials 0.000 description 1
- 101000918249 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) Exodeoxyribonuclease 1 Proteins 0.000 description 1
- 101100150136 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) SPO1 gene Proteins 0.000 description 1
- 241000277263 Salmo Species 0.000 description 1
- 241000277289 Salmo salar Species 0.000 description 1
- 206010039491 Sarcoma Diseases 0.000 description 1
- 241000209056 Secale Species 0.000 description 1
- 235000007238 Secale cereale Nutrition 0.000 description 1
- 244000082988 Secale cereale Species 0.000 description 1
- 108091081021 Sense strand Proteins 0.000 description 1
- 238000012300 Sequence Analysis Methods 0.000 description 1
- 241000207763 Solanum Species 0.000 description 1
- 235000002634 Solanum Nutrition 0.000 description 1
- 235000002597 Solanum melongena Nutrition 0.000 description 1
- 244000061458 Solanum melongena Species 0.000 description 1
- 244000061456 Solanum tuberosum Species 0.000 description 1
- 235000002595 Solanum tuberosum Nutrition 0.000 description 1
- 235000011684 Sorghum saccharatum Nutrition 0.000 description 1
- 244000062793 Sorghum vulgare Species 0.000 description 1
- 241000713675 Spumavirus Species 0.000 description 1
- 241000191967 Staphylococcus aureus Species 0.000 description 1
- 241000282898 Sus scrofa Species 0.000 description 1
- 244000269722 Thea sinensis Species 0.000 description 1
- 108700039575 Three prime repair exonuclease 2 Proteins 0.000 description 1
- 108010022394 Threonine synthase Proteins 0.000 description 1
- 102000006601 Thymidine Kinase Human genes 0.000 description 1
- 108020004440 Thymidine kinase Proteins 0.000 description 1
- 241000223259 Trichoderma Species 0.000 description 1
- 241000499912 Trichoderma reesei Species 0.000 description 1
- 241000209140 Triticum Species 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 244000098338 Triticum aestivum Species 0.000 description 1
- 235000007264 Triticum durum Nutrition 0.000 description 1
- 241000209143 Triticum turgidum subsp. durum Species 0.000 description 1
- 241001629556 Tulare apple mosaic virus Species 0.000 description 1
- 101710105527 Type II restriction enzyme FokI Proteins 0.000 description 1
- 101710117456 Type II restriction enzyme MspI Proteins 0.000 description 1
- 108091023045 Untranslated Region Proteins 0.000 description 1
- 206010046865 Vaccinia virus infection Diseases 0.000 description 1
- 241000711975 Vesicular stomatitis virus Species 0.000 description 1
- 241000607265 Vibrio vulnificus Species 0.000 description 1
- 101100004044 Vigna radiata var. radiata AUX22B gene Proteins 0.000 description 1
- 108020005202 Viral DNA Proteins 0.000 description 1
- 108020000999 Viral RNA Proteins 0.000 description 1
- 108010046377 Whey Proteins Proteins 0.000 description 1
- 241001276012 Wickerhamomyces ciferrii Species 0.000 description 1
- 101150042620 Xpc gene Proteins 0.000 description 1
- 241000209149 Zea Species 0.000 description 1
- 235000007244 Zea mays Nutrition 0.000 description 1
- 244000126002 Ziziphus vulgaris Species 0.000 description 1
- DGEZNRSVGBDHLK-UHFFFAOYSA-N [1,10]phenanthroline Chemical compound C1=CN=C2C3=NC=CC=C3C=CC2=C1 DGEZNRSVGBDHLK-UHFFFAOYSA-N 0.000 description 1
- 230000005856 abnormality Effects 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000002730 additional effect Effects 0.000 description 1
- 229960000643 adenine Drugs 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 239000011543 agarose gel Substances 0.000 description 1
- 244000193174 agave Species 0.000 description 1
- 125000003295 alanine group Chemical group N[C@@H](C)C(=O)* 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 1
- 229960000723 ampicillin Drugs 0.000 description 1
- 238000004873 anchoring Methods 0.000 description 1
- 230000003466 anti-cipated effect Effects 0.000 description 1
- 230000000692 anti-sense effect Effects 0.000 description 1
- 230000000840 anti-viral effect Effects 0.000 description 1
- 230000006907 apoptotic process Effects 0.000 description 1
- 235000009582 asparagine Nutrition 0.000 description 1
- 229960001230 asparagine Drugs 0.000 description 1
- 208000004668 avian leukosis Diseases 0.000 description 1
- 229940005348 bacillus firmus Drugs 0.000 description 1
- 229940097012 bacillus thuringiensis Drugs 0.000 description 1
- 239000012639 bacterial effector Substances 0.000 description 1
- 230000037429 base substitution Effects 0.000 description 1
- 108010081355 beta 2-Microglobulin Proteins 0.000 description 1
- 230000001588 bifunctional effect Effects 0.000 description 1
- 238000012742 biochemical analysis Methods 0.000 description 1
- 239000002551 biofuel Substances 0.000 description 1
- 238000001574 biopsy Methods 0.000 description 1
- 238000001815 biotherapy Methods 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 239000005018 casein Substances 0.000 description 1
- 239000003054 catalyst Substances 0.000 description 1
- 150000001768 cations Chemical class 0.000 description 1
- 230000018486 cell cycle phase Effects 0.000 description 1
- 230000032823 cell division Effects 0.000 description 1
- 230000006037 cell lysis Effects 0.000 description 1
- 210000004756 chromatid Anatomy 0.000 description 1
- 235000020971 citrus fruits Nutrition 0.000 description 1
- 239000002299 complementary DNA Substances 0.000 description 1
- 230000021615 conjugation Effects 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000009089 cytolysis Effects 0.000 description 1
- 210000000805 cytoplasm Anatomy 0.000 description 1
- 229940104302 cytosine Drugs 0.000 description 1
- 231100000135 cytotoxicity Toxicity 0.000 description 1
- 230000003013 cytotoxicity Effects 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000002716 delivery method Methods 0.000 description 1
- 239000000412 dendrimer Substances 0.000 description 1
- 229920000736 dendritic polymer Polymers 0.000 description 1
- 230000005595 deprotonation Effects 0.000 description 1
- 238000010537 deprotonation reaction Methods 0.000 description 1
- PQYUGUXEJHLOIL-UHFFFAOYSA-N diethoxysilyl triethyl silicate Chemical compound C(C)O[SiH](O[Si](OCC)(OCC)OCC)OCC PQYUGUXEJHLOIL-UHFFFAOYSA-N 0.000 description 1
- 102000004419 dihydrofolate reductase Human genes 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 230000000447 dimerizing effect Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000012377 drug delivery Methods 0.000 description 1
- 239000002961 echo contrast media Substances 0.000 description 1
- 231100001129 embryonic lethality Toxicity 0.000 description 1
- 210000001671 embryonic stem cell Anatomy 0.000 description 1
- 239000000839 emulsion Substances 0.000 description 1
- 108010047964 endonuclease G Proteins 0.000 description 1
- ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 1
- 108010052305 exodeoxyribonuclease III Proteins 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 238000012224 gene deletion Methods 0.000 description 1
- 238000012239 gene modification Methods 0.000 description 1
- 238000012252 genetic analysis Methods 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 102000005396 glutamine synthetase Human genes 0.000 description 1
- 108020002326 glutamine synthetase Proteins 0.000 description 1
- 229940047650 haemophilus influenzae Drugs 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 208000006454 hepatitis Diseases 0.000 description 1
- 231100000283 hepatitis Toxicity 0.000 description 1
- 238000013537 high throughput screening Methods 0.000 description 1
- 102000049902 human IL2RG Human genes 0.000 description 1
- 102000049029 human RBBP8 Human genes 0.000 description 1
- 210000005260 human cell Anatomy 0.000 description 1
- 210000003917 human chromosome Anatomy 0.000 description 1
- 108010002685 hygromycin-B kinase Proteins 0.000 description 1
- 210000001822 immobilized cell Anatomy 0.000 description 1
- 230000028993 immune response Effects 0.000 description 1
- 230000005847 immunogenicity Effects 0.000 description 1
- 238000009169 immunotherapy Methods 0.000 description 1
- 230000001771 impaired effect Effects 0.000 description 1
- 230000001976 improved effect Effects 0.000 description 1
- 238000000126 in silico method Methods 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 239000000543 intermediate Substances 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 238000011813 knockout mouse model Methods 0.000 description 1
- 101150066555 lacZ gene Proteins 0.000 description 1
- 230000003902 lesion Effects 0.000 description 1
- 239000002502 liposome Substances 0.000 description 1
- 239000006166 lysate Substances 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 102000016470 mariner transposase Human genes 0.000 description 1
- 108060004631 mariner transposase Proteins 0.000 description 1
- 230000013011 mating Effects 0.000 description 1
- 230000012092 mating type switching Effects 0.000 description 1
- 230000021121 meiosis Effects 0.000 description 1
- 108020004999 messenger RNA Proteins 0.000 description 1
- 150000002739 metals Chemical class 0.000 description 1
- 238000000520 microinjection Methods 0.000 description 1
- 230000002438 mitochondrial effect Effects 0.000 description 1
- 230000037230 mobility Effects 0.000 description 1
- 238000009126 molecular therapy Methods 0.000 description 1
- 231100000243 mutagenic effect Toxicity 0.000 description 1
- 239000002105 nanoparticle Substances 0.000 description 1
- 230000004766 neurogenesis Effects 0.000 description 1
- 230000012223 nuclear import Effects 0.000 description 1
- 238000007899 nucleic acid hybridization Methods 0.000 description 1
- 239000002777 nucleoside Substances 0.000 description 1
- 150000003833 nucleoside derivatives Chemical class 0.000 description 1
- 238000002515 oligonucleotide synthesis Methods 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000010412 perfusion Effects 0.000 description 1
- 230000008823 permeabilization Effects 0.000 description 1
- 230000002688 persistence Effects 0.000 description 1
- 239000000546 pharmaceutical excipient Substances 0.000 description 1
- 108010085336 phosphoribosyl-AMP cyclohydrolase Proteins 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 230000008488 polyadenylation Effects 0.000 description 1
- 229920002647 polyamide Polymers 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 230000004952 protein activity Effects 0.000 description 1
- 230000016434 protein splicing Effects 0.000 description 1
- 239000002213 purine nucleotide Substances 0.000 description 1
- 239000002719 pyrimidine nucleotide Substances 0.000 description 1
- 108020003175 receptors Proteins 0.000 description 1
- 102000005962 receptors Human genes 0.000 description 1
- 231100000916 relative toxicity Toxicity 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- JQXXHWHPUNPDRT-WLSIYKJHSA-N rifampicin Chemical compound O([C@](C1=O)(C)O/C=C/[C@@H]([C@H]([C@@H](OC(C)=O)[C@H](C)[C@H](O)[C@H](C)[C@@H](O)[C@@H](C)\C=C\C=C(C)/C(=O)NC=2C(O)=C3C([O-])=C4C)C)OC)C4=C1C3=C(O)C=2\C=N\N1CC[NH+](C)CC1 JQXXHWHPUNPDRT-WLSIYKJHSA-N 0.000 description 1
- 229960001225 rifampicin Drugs 0.000 description 1
- 210000003296 saliva Anatomy 0.000 description 1
- 230000035939 shock Effects 0.000 description 1
- 230000003584 silencer Effects 0.000 description 1
- 238000002741 site-directed mutagenesis Methods 0.000 description 1
- 210000002027 skeletal muscle Anatomy 0.000 description 1
- 238000010583 slow cooling Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 210000000130 stem cell Anatomy 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 238000012916 structural analysis Methods 0.000 description 1
- 230000006918 subunit interaction Effects 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 238000001356 surgical procedure Methods 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 230000002123 temporal effect Effects 0.000 description 1
- OFVLGDICTFRJMM-WESIUVDSSA-N tetracycline Chemical compound C1=CC=C2[C@](O)(C)[C@H]3C[C@H]4[C@H](N(C)C)C(O)=C(C(N)=O)C(=O)[C@@]4(O)C(O)=C3C(=O)C2=C1O OFVLGDICTFRJMM-WESIUVDSSA-N 0.000 description 1
- 229930101283 tetracycline Natural products 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 229940113082 thymine Drugs 0.000 description 1
- 230000005030 transcription termination Effects 0.000 description 1
- 230000002103 transcriptional effect Effects 0.000 description 1
- 238000003146 transient transfection Methods 0.000 description 1
- 230000001960 triggered effect Effects 0.000 description 1
- 239000002753 trypsin inhibitor Substances 0.000 description 1
- 201000008827 tuberculosis Diseases 0.000 description 1
- 231100000588 tumorigenic Toxicity 0.000 description 1
- 230000000381 tumorigenic effect Effects 0.000 description 1
- 238000010396 two-hybrid screening Methods 0.000 description 1
- 241000701447 unidentified baculovirus Species 0.000 description 1
- 241001529453 unidentified herpesvirus Species 0.000 description 1
- 241000712461 unidentified influenza virus Species 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
- 208000007089 vaccinia Diseases 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/10—Cells modified by introduction of foreign genetic material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
- C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
- C07K14/4702—Regulators; Modulating activity
- C07K14/4703—Inhibitors; Suppressors
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/07—Fusion polypeptide containing a localisation/targetting motif containing a mitochondrial localisation signal
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Toxicology (AREA)
- Plant Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Crystallography & Structural Chemistry (AREA)
- Immunology (AREA)
- Animal Behavior & Ethology (AREA)
- Veterinary Medicine (AREA)
- Pharmacology & Pharmacy (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Analytical Chemistry (AREA)
- Public Health (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Applications Claiming Priority (15)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161472065P | 2011-04-05 | 2011-04-05 | |
| US61/472,065 | 2011-04-05 | ||
| US201161496454P | 2011-06-13 | 2011-06-13 | |
| US61/496,454 | 2011-06-13 | ||
| US201161499047P | 2011-06-20 | 2011-06-20 | |
| US201161499043P | 2011-06-20 | 2011-06-20 | |
| US61/499,043 | 2011-06-20 | ||
| US61/499,047 | 2011-06-20 | ||
| US201161533098P | 2011-09-09 | 2011-09-09 | |
| US201161533123P | 2011-09-09 | 2011-09-09 | |
| US61/533,123 | 2011-09-09 | ||
| US61/533,098 | 2011-09-09 | ||
| US201161579544P | 2011-12-22 | 2011-12-22 | |
| US61/579,544 | 2011-12-22 | ||
| CA2832534A CA2832534C (en) | 2011-04-05 | 2012-04-05 | Method for the generation of compact tale-nucleases and uses thereof |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2832534A Division CA2832534C (en) | 2011-04-05 | 2012-04-05 | Method for the generation of compact tale-nucleases and uses thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA3111953A1 CA3111953A1 (en) | 2012-10-11 |
| CA3111953C true CA3111953C (en) | 2023-10-24 |
Family
ID=45976522
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA3111953A Active CA3111953C (en) | 2011-04-05 | 2012-04-05 | Method for the generation of compact tale-nucleases and uses thereof |
| CA2832534A Active CA2832534C (en) | 2011-04-05 | 2012-04-05 | Method for the generation of compact tale-nucleases and uses thereof |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2832534A Active CA2832534C (en) | 2011-04-05 | 2012-04-05 | Method for the generation of compact tale-nucleases and uses thereof |
Country Status (13)
| Country | Link |
|---|---|
| US (5) | US20140115726A1 (enExample) |
| EP (3) | EP2694091B1 (enExample) |
| JP (3) | JP5996630B2 (enExample) |
| KR (1) | KR101982360B1 (enExample) |
| CN (1) | CN103608027B (enExample) |
| AU (2) | AU2012240110B2 (enExample) |
| CA (2) | CA3111953C (enExample) |
| DK (1) | DK2694091T3 (enExample) |
| ES (1) | ES2728436T3 (enExample) |
| HK (1) | HK1253952A1 (enExample) |
| IL (1) | IL228747B (enExample) |
| SG (2) | SG194115A1 (enExample) |
| WO (2) | WO2012138939A1 (enExample) |
Families Citing this family (269)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA3059768A1 (en) | 2008-09-05 | 2010-03-11 | President And Fellows Of Harvard College | Continuous directed evolution of proteins and nucleic acids |
| AU2011265733B2 (en) * | 2010-06-14 | 2014-04-17 | Iowa State University Research Foundation, Inc. | Nuclease activity of TAL effector and Foki fusion protein |
| JP6088438B2 (ja) | 2010-12-22 | 2017-03-01 | プレジデント アンド フェローズ オブ ハーバード カレッジ | 連続的定向進化 |
| EP3461896B1 (en) | 2011-07-15 | 2023-11-29 | The General Hospital Corporation | Methods of transcription activator like effector assembly |
| WO2013066438A2 (en) | 2011-07-22 | 2013-05-10 | President And Fellows Of Harvard College | Evaluation and improvement of nuclease cleavage specificity |
| US20150017728A1 (en) * | 2011-09-23 | 2015-01-15 | Iowa State University Research Foundation, Inc | Monomer architecture of tal nuclease or zinc finger nuclease for dna modification |
| EP2776560A2 (en) | 2011-11-07 | 2014-09-17 | The University of Western Ontario | Endonuclease for genome editing |
| HK1200871A1 (en) | 2011-11-16 | 2015-08-14 | Sangamo Therapeutics, Inc. | Modified dna-binding proteins and uses thereof |
| US9688997B2 (en) | 2011-12-29 | 2017-06-27 | Iowa State University Research Foundation, Inc. | Genetically modified plants with resistance to Xanthomonas and other bacterial plant pathogens |
| DK2825190T3 (en) * | 2012-03-15 | 2017-02-27 | Cellectis | REPEAT VARIABLE DIRESTS FOR TARGETING NUCLEOTIDES |
| US10704021B2 (en) | 2012-03-15 | 2020-07-07 | Flodesign Sonics, Inc. | Acoustic perfusion devices |
| ES2828663T3 (es) * | 2012-04-18 | 2021-05-27 | Univ Leland Stanford Junior | Dirección genética no disruptiva |
| WO2013160230A1 (en) | 2012-04-23 | 2013-10-31 | Bayer Cropscience Nv | Targeted genome engineering in plants |
| US20150017136A1 (en) | 2013-07-15 | 2015-01-15 | Cellectis | Methods for engineering allogeneic and highly active t cell for immunotherapy |
| CA3133545C (en) | 2012-05-25 | 2023-08-08 | Cellectis | Use of pre t alpha or functional variant thereof for expanding tcr alpha deficient t cells |
| US9890364B2 (en) | 2012-05-29 | 2018-02-13 | The General Hospital Corporation | TAL-Tet1 fusion proteins and methods of use thereof |
| US10815500B2 (en) * | 2012-06-05 | 2020-10-27 | Cellectis | Transcription activator-like effector (TALE) fusion protein |
| US20150225734A1 (en) | 2012-06-19 | 2015-08-13 | Regents Of The University Of Minnesota | Gene targeting in plants using dna viruses |
| US9091827B2 (en) | 2012-07-09 | 2015-07-28 | Luxtera, Inc. | Method and system for grating couplers incorporating perturbed waveguides |
| AU2013312838B2 (en) | 2012-09-04 | 2018-11-29 | Cellectis | Multi-chain chimeric antigen receptor and uses thereof |
| CN103668470B (zh) * | 2012-09-12 | 2015-07-29 | 上海斯丹赛生物技术有限公司 | 一种dna文库及构建转录激活子样效应因子核酸酶质粒的方法 |
| EP2906602B1 (en) | 2012-10-12 | 2019-01-16 | The General Hospital Corporation | Transcription activator-like effector (tale) - lysine-specific demethylase 1 (lsd1) fusion proteins |
| KR101656236B1 (ko) | 2012-10-23 | 2016-09-12 | 주식회사 툴젠 | 표적 DNA에 특이적인 가이드 RNA 및 Cas 단백질을 암호화하는 핵산 또는 Cas 단백질을 포함하는, 표적 DNA를 절단하기 위한 조성물 및 이의 용도 |
| US20150291967A1 (en) | 2012-10-31 | 2015-10-15 | Luc Mathis | Coupling herbicide resistance with targeted insertion of transgenes in plants |
| BR122019025681B1 (pt) * | 2012-11-01 | 2023-04-18 | Factor Bioscience Inc | Método para inserir uma sequência de ácido nucleico em uma localização segura de um genoma de uma célula |
| CN105051204B (zh) | 2012-11-16 | 2023-07-21 | 波赛伊达治疗学股份有限公司 | 位点特异性酶和使用方法 |
| AU2013346504A1 (en) | 2012-11-16 | 2015-06-18 | Cellectis | Method for targeted modification of algae genomes |
| WO2014102688A1 (en) | 2012-12-27 | 2014-07-03 | Cellectis | New design matrix for improvement of homology-directed gene targeting |
| EP2951295B1 (en) | 2013-02-01 | 2018-08-01 | Cellectis | Tevi chimeric endonucleases and their preferential cleavage sites |
| US10676749B2 (en) | 2013-02-07 | 2020-06-09 | The General Hospital Corporation | Tale transcriptional activators |
| WO2014128659A1 (en) | 2013-02-21 | 2014-08-28 | Cellectis | Method to counter-select cells or organisms by linking loci to nuclease components |
| AU2014235794A1 (en) * | 2013-03-14 | 2015-10-22 | Caribou Biosciences, Inc. | Compositions and methods of nucleic acid-targeting nucleic acids |
| EP2975942B1 (en) * | 2013-03-21 | 2018-08-08 | Sangamo Therapeutics, Inc. | Targeted disruption of t cell receptor genes using engineered zinc finger protein nucleases |
| WO2014161821A1 (en) | 2013-04-02 | 2014-10-09 | Bayer Cropscience Nv | Targeted genome engineering in eukaryotes |
| WO2014169810A1 (zh) * | 2013-04-16 | 2014-10-23 | 深圳华大基因科技服务有限公司 | 分离的寡核苷酸及其用途 |
| RS65484B1 (sr) | 2013-05-13 | 2024-05-31 | Cellectis | Cd19 specifični himerni antigenski receptor i njegove primene |
| AU2014266833B2 (en) | 2013-05-13 | 2020-07-02 | Cellectis | Methods for engineering highly active T cell for immunotherapy |
| EP3004149B1 (en) * | 2013-05-31 | 2018-12-19 | Cellectis S.A. | A laglidadg homing endonuclease cleaving the c-c chemokine receptor type-5 (ccr5) gene and uses thereof |
| AU2014273091B2 (en) * | 2013-05-31 | 2019-12-12 | Cellectis | A LAGLIDADG homing endonuclease cleaving the T cell receptor alpha gene and uses thereof |
| WO2014199358A1 (en) * | 2013-06-14 | 2014-12-18 | Cellectis | Methods for non-transgenic genome editing in plants |
| AU2014301147B2 (en) * | 2013-06-25 | 2020-07-30 | Cellectis | Modified diatoms for biofuel production |
| FR3008106A1 (fr) * | 2013-07-05 | 2015-01-09 | Agronomique Inst Nat Rech | Methode d'induction d'une recombinaison meiotique ciblee |
| US10782479B2 (en) | 2013-07-08 | 2020-09-22 | Luxtera Llc | Method and system for mode converters for grating couplers |
| US10006011B2 (en) | 2013-08-09 | 2018-06-26 | Hiroshima University | Polypeptide containing DNA-binding domain |
| US20150044192A1 (en) | 2013-08-09 | 2015-02-12 | President And Fellows Of Harvard College | Methods for identifying a target site of a cas9 nuclease |
| US9359599B2 (en) * | 2013-08-22 | 2016-06-07 | President And Fellows Of Harvard College | Engineered transcription activator-like effector (TALE) domains and uses thereof |
| US9340799B2 (en) | 2013-09-06 | 2016-05-17 | President And Fellows Of Harvard College | MRNA-sensing switchable gRNAs |
| US9737604B2 (en) | 2013-09-06 | 2017-08-22 | President And Fellows Of Harvard College | Use of cationic lipids to deliver CAS9 |
| US9388430B2 (en) | 2013-09-06 | 2016-07-12 | President And Fellows Of Harvard College | Cas9-recombinase fusion proteins and uses thereof |
| AU2014321215B2 (en) * | 2013-09-23 | 2020-07-16 | Rensselaer Polytechnic Institute | Nanoparticle-mediated gene delivery, genomic editing and ligand-targeted modification in various cell populations |
| US9476884B2 (en) | 2013-10-04 | 2016-10-25 | University Of Massachusetts | Hybridization- independent labeling of repetitive DNA sequence in human chromosomes |
| EP3060658B1 (en) * | 2013-10-25 | 2020-07-15 | Cellectis | Design of rare-cutting endonucleases for efficient and specific targeting dna sequences comprising highly repetitive motives |
| KR20230054509A (ko) | 2013-11-07 | 2023-04-24 | 에디타스 메디신, 인코포레이티드 | 지배적인 gRNA를 이용하는 CRISPR-관련 방법 및 조성물 |
| US11053481B2 (en) | 2013-12-12 | 2021-07-06 | President And Fellows Of Harvard College | Fusions of Cas9 domains and nucleic acid-editing domains |
| CA2935960C (en) | 2014-01-08 | 2023-01-10 | Bart Lipkens | Acoustophoresis device with dual acoustophoretic chamber |
| WO2015134121A2 (en) | 2014-01-20 | 2015-09-11 | President And Fellows Of Harvard College | Negative selection and stringency modulation in continuous evolution systems |
| JP6673838B2 (ja) | 2014-02-14 | 2020-04-01 | セレクティスCellectis | 免疫細胞と病的細胞の両方に存在する抗原を標的とするように操作された、免疫療法のための細胞 |
| ES2782125T3 (es) | 2014-03-11 | 2020-09-10 | Cellectis | Método para generar linfocitos T compatibles para trasplante alogénico |
| ES2740903T3 (es) | 2014-03-19 | 2020-02-07 | Cellectis | Receptores antigénicos quiméricos específicos de CD123 para inmunoterapia del cáncer |
| CA2939847C (en) | 2014-03-21 | 2023-09-05 | The Board Of Trustees Of The Leland Stanford Junior University | Genome editing without nucleases |
| WO2015155341A1 (en) | 2014-04-11 | 2015-10-15 | Cellectis | Method for generating immune cells resistant to arginine and/or tryptophan depleted microenvironment |
| EP3157328B1 (en) * | 2014-06-17 | 2021-08-04 | Poseida Therapeutics, Inc. | A method for directing proteins to specific loci in the genome and uses thereof |
| BR112017001821A2 (pt) | 2014-07-29 | 2017-11-21 | Cellectis | receptores de antígenos quiméricos específicos de ror1 (ntrkr1) para imunoterapia de câncer |
| AU2015298571B2 (en) | 2014-07-30 | 2020-09-03 | President And Fellows Of Harvard College | Cas9 proteins including ligand-dependent inteins |
| WO2016016343A1 (en) | 2014-07-31 | 2016-02-04 | Cellectis | Ror1 specific multi-chain chimeric antigen receptor |
| EP3189073B2 (en) | 2014-09-04 | 2025-06-11 | Cellectis | Trophoblast glycoprotein (5t4, tpbg) specific chimeric antigen receptors for cancer immunotherapy |
| WO2016077052A2 (en) | 2014-10-22 | 2016-05-19 | President And Fellows Of Harvard College | Evolution of proteases |
| CA2973529A1 (en) | 2015-01-26 | 2016-08-04 | Cellectis | Cll1-specific multi-chain chimeric antigen receptor |
| EP3543339A1 (en) | 2015-02-13 | 2019-09-25 | Factor Bioscience Inc. | Nucleic acid products and methods of administration thereof |
| US20160244784A1 (en) * | 2015-02-15 | 2016-08-25 | Massachusetts Institute Of Technology | Population-Hastened Assembly Genetic Engineering |
| US11186824B2 (en) | 2015-03-11 | 2021-11-30 | Cellectis | Methods for engineering allogeneic T cell to increase their persistence and/or engraftment into patients |
| US11299729B2 (en) | 2015-04-17 | 2022-04-12 | President And Fellows Of Harvard College | Vector-based mutagenesis system |
| US11708572B2 (en) | 2015-04-29 | 2023-07-25 | Flodesign Sonics, Inc. | Acoustic cell separation techniques and processes |
| US11377651B2 (en) | 2016-10-19 | 2022-07-05 | Flodesign Sonics, Inc. | Cell therapy processes utilizing acoustophoresis |
| WO2016179112A1 (en) | 2015-05-01 | 2016-11-10 | Precision Biosciences, Inc. | Precise deletion of chromoscomal sequences in vivo and treatment of nucleotide repeat expansion disorders using engineered nucleases |
| EP3298046B1 (en) | 2015-05-20 | 2025-02-12 | Cellectis | Anti-gd3 specific chimeric antigen receptors for cancer immunotherapy |
| DK3310909T3 (da) | 2015-06-17 | 2021-09-13 | Poseida Therapeutics Inc | Sammensætninger og fremgangsmåder til at føre proteiner til specifikke loci i genomet |
| US20180171298A1 (en) | 2015-06-30 | 2018-06-21 | Cellectis | Methods for improving functionality in nk cell by gene inactivation using specific endonuclease |
| WO2017015545A1 (en) | 2015-07-22 | 2017-01-26 | President And Fellows Of Harvard College | Evolution of site-specific recombinases |
| US11524983B2 (en) | 2015-07-23 | 2022-12-13 | President And Fellows Of Harvard College | Evolution of Bt toxins |
| WO2017019895A1 (en) | 2015-07-30 | 2017-02-02 | President And Fellows Of Harvard College | Evolution of talens |
| CN104962523B (zh) * | 2015-08-07 | 2018-05-25 | 苏州大学 | 一种测定非同源末端连接修复活性的方法 |
| WO2017025323A1 (en) | 2015-08-11 | 2017-02-16 | Cellectis | Cells for immunotherapy engineered for targeting cd38 antigen and for cd38 gene inactivation |
| US12043852B2 (en) | 2015-10-23 | 2024-07-23 | President And Fellows Of Harvard College | Evolved Cas9 proteins for gene editing |
| WO2017079428A1 (en) | 2015-11-04 | 2017-05-11 | President And Fellows Of Harvard College | Site specific germline modification |
| EP3429633B1 (en) | 2016-04-15 | 2021-02-24 | Cellectis | A method of engineering drug-specific hypersensitive t-cells for immunotherapy by gene inactivation |
| US20190328783A1 (en) | 2016-04-15 | 2019-10-31 | Cellectis | A method of engineering prodrug-specific hypersensitive t-cells for immunotherapy by gene expression |
| US11214789B2 (en) | 2016-05-03 | 2022-01-04 | Flodesign Sonics, Inc. | Concentration and washing of particles with acoustics |
| US11293033B2 (en) | 2016-05-18 | 2022-04-05 | Amyris, Inc. | Compositions and methods for genomic integration of nucleic acids into exogenous landing pads |
| EA201892624A1 (ru) | 2016-05-26 | 2019-06-28 | Нунхемс Б.В. | Растения, дающие бессемянные плоды |
| KR20250103795A (ko) | 2016-08-03 | 2025-07-07 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 아데노신 핵염기 편집제 및 그의 용도 |
| CN109804066A (zh) | 2016-08-09 | 2019-05-24 | 哈佛大学的校长及成员们 | 可编程cas9-重组酶融合蛋白及其用途 |
| UY40582A (es) | 2016-08-17 | 2024-01-15 | Monsanto Technology Llc | MÉTODOS, COMPOSICIONES y PLANTAS MEDIANTE LA MANIPULACIÓN DEL METABOLISMO DE GIBERELINA PARA AUMENTAR EL RENDIMIENTO COSECHABLE |
| CN109803977B (zh) | 2016-08-17 | 2023-03-17 | 菲克特生物科学股份有限公司 | 核酸产品及其施用方法 |
| US11542509B2 (en) | 2016-08-24 | 2023-01-03 | President And Fellows Of Harvard College | Incorporation of unnatural amino acids into proteins using base editing |
| JP6887492B6 (ja) | 2016-10-04 | 2021-07-14 | プレシジョン バイオサイエンシズ,インク. | 遺伝子改変細胞で使用するための共刺激ドメイン |
| AU2017342543B2 (en) | 2016-10-14 | 2024-06-27 | President And Fellows Of Harvard College | AAV delivery of nucleobase editors |
| US20180135080A1 (en) * | 2016-11-15 | 2018-05-17 | Genomic Vision Sa | Method for the monitoring of modified nucleases induced-gene editing events by molecular combing |
| JP7317706B2 (ja) | 2016-12-14 | 2023-07-31 | リガンダル インコーポレイテッド | 核酸およびタンパク質ペイロード送達のための方法および組成物 |
| WO2018115189A1 (en) | 2016-12-21 | 2018-06-28 | Cellectis | Stably enginereed proteasome inhibitor resistant immune cells for immunotherapy |
| WO2018119359A1 (en) | 2016-12-23 | 2018-06-28 | President And Fellows Of Harvard College | Editing of ccr5 receptor gene to protect against hiv infection |
| EP3592853A1 (en) | 2017-03-09 | 2020-01-15 | President and Fellows of Harvard College | Suppression of pain by gene editing |
| EP3592381A1 (en) | 2017-03-09 | 2020-01-15 | President and Fellows of Harvard College | Cancer vaccine |
| WO2018162702A1 (en) | 2017-03-10 | 2018-09-13 | Institut National De La Sante Et De La Recherche Medicale (Inserm) | Nuclease fusions for enhancing genome editing by homology-directed transgene integration |
| KR20190127797A (ko) | 2017-03-10 | 2019-11-13 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 시토신에서 구아닌으로의 염기 편집제 |
| JP6901293B2 (ja) * | 2017-03-15 | 2021-07-14 | 株式会社三共 | 遊技用装置 |
| CA3057192A1 (en) | 2017-03-23 | 2018-09-27 | President And Fellows Of Harvard College | Nucleobase editors comprising nucleic acid programmable dna binding proteins |
| EP3592379B1 (en) | 2017-03-31 | 2024-04-24 | Cellectis SA | Universal anti-cd22 chimeric antigen receptor engineered immune cells |
| WO2018208837A1 (en) | 2017-05-08 | 2018-11-15 | Precision Biosciences, Inc. | Nucleic acid molecules encoding an engineered antigen receptor and an inhibitory nucleic acid molecule and methods of use thereof |
| WO2018209320A1 (en) | 2017-05-12 | 2018-11-15 | President And Fellows Of Harvard College | Aptazyme-embedded guide rnas for use with crispr-cas9 in genome editing and transcriptional activation |
| KR102590466B1 (ko) | 2017-05-25 | 2023-10-19 | 2세븐티 바이오, 인코포레이티드 | Cblb 엔도뉴클레아제 변이체, 조성물 및 사용 방법 |
| JP2020530277A (ja) | 2017-06-30 | 2020-10-22 | セレクティスCellectis | 反復投与のための細胞免疫療法 |
| WO2019010164A1 (en) | 2017-07-06 | 2019-01-10 | President And Fellows Of Harvard College | Evolution of trna synthetases |
| CN111801345A (zh) | 2017-07-28 | 2020-10-20 | 哈佛大学的校长及成员们 | 使用噬菌体辅助连续进化(pace)的进化碱基编辑器的方法和组合物 |
| US12060553B2 (en) | 2017-08-25 | 2024-08-13 | President And Fellows Of Harvard College | Evolution of BoNT peptidases |
| WO2019139645A2 (en) | 2017-08-30 | 2019-07-18 | President And Fellows Of Harvard College | High efficiency base editors comprising gam |
| WO2019056002A1 (en) | 2017-09-18 | 2019-03-21 | President And Fellows Of Harvard College | CONTINUOUS EVOLUTION FOR STABILIZED PROTEINS |
| US20200224194A1 (en) * | 2017-09-20 | 2020-07-16 | Helix Nanotechnologies, Inc. | Expression systems that facilitate nucleic acid delivery and methods of use |
| WO2019070856A1 (en) | 2017-10-03 | 2019-04-11 | Precision Biosciences, Inc. | MODIFIED EPIDERMAL GROWTH FACTOR RECEPTOR PEPTIDES FOR USE IN GENETICALLY MODIFIED CELLS |
| WO2019072824A1 (en) | 2017-10-09 | 2019-04-18 | Cellectis | IMPROVED ANTI-CD123 CAR IN UNIVERSAL MODIFIED IMMUNE T LYMPHOCYTES |
| CA3082251A1 (en) | 2017-10-16 | 2019-04-25 | The Broad Institute, Inc. | Uses of adenosine base editors |
| KR20200100060A (ko) | 2017-11-17 | 2020-08-25 | 이오반스 바이오테라퓨틱스, 인크. | 미세 바늘 흡인물 및 소형 생검물로부터의 til 확장 |
| EP3714041A1 (en) | 2017-11-22 | 2020-09-30 | Iovance Biotherapeutics, Inc. | Expansion of peripheral blood lymphocytes (pbls) from peripheral blood |
| KR20220066413A (ko) | 2017-12-14 | 2022-05-24 | 프로디자인 소닉스, 인크. | 음향 트랜스듀서 구동기 및 제어기 |
| EP3724214A4 (en) | 2017-12-15 | 2021-09-01 | The Broad Institute Inc. | SYSTEMS AND METHODS FOR PREDICTING REPAIR RESULTS IN GENETIC ENGINEERING |
| EP3501268B1 (en) | 2017-12-22 | 2021-09-15 | KWS SAAT SE & Co. KGaA | Regeneration of plants in the presence of histone deacetylase inhibitors |
| EP3508581A1 (en) | 2018-01-03 | 2019-07-10 | Kws Saat Se | Regeneration of genetically modified plants |
| MX2020007466A (es) | 2018-01-12 | 2020-11-12 | Basf Se | Gen subyacente al qtl de la cantidad de espiguillas por espiga en trigo en el cromosoma 7a. |
| US20230158070A1 (en) | 2018-01-30 | 2023-05-25 | Cellectis | Combination comprising allogeneic immune cells deficient for an antigen present on both t-cells and pathological cells and therapeutic antibody against said antigen |
| UY38094A (es) | 2018-02-15 | 2019-10-01 | Monsanto Technology Llc | Métodos mejorados para la producción de semillas de maíz híbridas |
| WO2019161151A1 (en) | 2018-02-15 | 2019-08-22 | Monsanto Technology Llc | Compositions and methods for improving crop yields through trait stacking |
| EP3545756A1 (en) | 2018-03-28 | 2019-10-02 | KWS SAAT SE & Co. KGaA | Regeneration of plants in the presence of inhibitors of the histone methyltransferase ezh2 |
| SG11202009446TA (en) | 2018-04-05 | 2020-10-29 | Juno Therapeutics Inc | T cells expressing a recombinant receptor, related polynucleotides and methods |
| MA52656A (fr) | 2018-04-05 | 2021-02-17 | Editas Medicine Inc | Procédés de production de cellules exprimant un récepteur recombinant et compositions associées |
| TWI831776B (zh) | 2018-04-27 | 2024-02-11 | 美商艾歐凡斯生物治療公司 | 腫瘤浸潤性淋巴細胞之基因編輯和彼於免疫治療之用途 |
| EP3788154A1 (en) | 2018-05-02 | 2021-03-10 | Cellectis | Engineering wheat with increased dietary fiber |
| PT3567111T (pt) | 2018-05-09 | 2025-10-23 | Kws Saat Se & Co Kgaa | Gene para resistência a um patógeno do género heterodera |
| WO2019226953A1 (en) | 2018-05-23 | 2019-11-28 | The Broad Institute, Inc. | Base editors and uses thereof |
| BR112020025439A2 (pt) | 2018-06-14 | 2021-03-16 | Bluebird Bio, Inc. | Receptores de antígenos quiméricos cd79a |
| WO2019241649A1 (en) | 2018-06-14 | 2019-12-19 | President And Fellows Of Harvard College | Evolution of cytidine deaminases |
| EP3807301A1 (en) | 2018-06-15 | 2021-04-21 | KWS SAAT SE & Co. KGaA | Methods for improving genome engineering and regeneration in plant ii |
| CN119265223A (zh) | 2018-06-15 | 2025-01-07 | 科沃施种子欧洲股份两合公司 | 增强基因组工程化效率的方法 |
| CN112566924B (zh) | 2018-06-15 | 2024-10-29 | 科沃施种子欧洲股份两合公司 | 改善植物中基因组工程化和再生的方法 |
| EP3806619A1 (en) | 2018-06-15 | 2021-04-21 | Nunhems B.V. | Seedless watermelon plants comprising modifications in an abc transporter gene |
| US20210268028A1 (en) | 2018-07-02 | 2021-09-02 | Cellectis | Chimeric antigen receptors (car)-expressing cells and combination treatment for immunotherapy of patients with relapse refractory adverse genetic risk aml |
| EP3623379A1 (en) | 2018-09-11 | 2020-03-18 | KWS SAAT SE & Co. KGaA | Beet necrotic yellow vein virus (bnyvv)-resistance modifying gene |
| WO2020072059A1 (en) | 2018-10-04 | 2020-04-09 | Bluebird Bio, Inc. | Cblb endonuclease variants, compositions, and methods of use |
| US12281338B2 (en) | 2018-10-29 | 2025-04-22 | The Broad Institute, Inc. | Nucleobase editors comprising GeoCas9 and uses thereof |
| WO2020096989A1 (en) | 2018-11-05 | 2020-05-14 | Iovance Biotherapeutics, Inc. | Treatment of nsclc patients refractory for anti-pd-1 antibody |
| AU2019375416A1 (en) | 2018-11-05 | 2021-05-27 | Iovance Biotherapeutics, Inc. | Selection of improved tumor reactive T-cells |
| WO2020096988A2 (en) | 2018-11-05 | 2020-05-14 | Iovance Biotherapeutics, Inc. | Processes for production of tumor infiltrating lymphocytes and uses of the same in immunotherapy |
| WO2020096927A1 (en) | 2018-11-05 | 2020-05-14 | Iovance Biotherapeutics, Inc. | Expansion of tils utilizing akt pathway inhibitors |
| US12274205B2 (en) | 2018-12-12 | 2025-04-15 | Monsanto Technology Llc | Delayed harvest of short stature corn plants |
| CA3123392A1 (en) | 2018-12-19 | 2020-06-25 | Iovance Biotherapeutics, Inc. | Methods of expanding tumor infiltrating lymphocytes using engineered cytokine receptor pairs and uses thereof |
| EP3898661A1 (en) | 2018-12-21 | 2021-10-27 | Precision BioSciences, Inc. | Genetic modification of the hydroxyacid oxidase 1 gene for treatment of primary hyperoxaluria |
| US12351837B2 (en) | 2019-01-23 | 2025-07-08 | The Broad Institute, Inc. | Supernegatively charged proteins and uses thereof |
| US20220112511A1 (en) | 2019-01-29 | 2022-04-14 | The University Of Warwick | Methods for enhancing genome engineering efficiency |
| MX2021010288A (es) | 2019-03-01 | 2021-09-23 | Iovance Biotherapeutics Inc | Expansion de linfocitos infiltrantes de tumores a partir de tumores liquidos y usos terapeuticos de los mismos. |
| EP3708651A1 (en) | 2019-03-12 | 2020-09-16 | KWS SAAT SE & Co. KGaA | Improving plant regeneration |
| DE112020001306T5 (de) | 2019-03-19 | 2022-01-27 | Massachusetts Institute Of Technology | Verfahren und zusammensetzungen zur editierung von nukleotidsequenzen |
| US11427814B2 (en) | 2019-03-26 | 2022-08-30 | Encodia, Inc. | Modified cleavases, uses thereof and related kits |
| MX2021011726A (es) * | 2019-03-26 | 2021-10-22 | Encodia Inc | Escindasas modificadas, usos de las mismas y kits relacionados. |
| CN116200342A (zh) | 2019-04-03 | 2023-06-02 | 精密生物科学公司 | 包含microRNA适应的shRNA(shRNAmiR)的遗传修饰的免疫细胞 |
| WO2020206231A1 (en) | 2019-04-05 | 2020-10-08 | Precision Biosciences, Inc. | Methods of preparing populations of genetically-modified immune cells |
| US12473543B2 (en) | 2019-04-17 | 2025-11-18 | The Broad Institute, Inc. | Adenine base editors with reduced off-target effects |
| KR20220016474A (ko) | 2019-05-01 | 2022-02-09 | 주노 쎄러퓨티크스 인코퍼레이티드 | 변형된 cd247 유전자 자리로부터 키메라 수용체를 발현하는 세포, 관련 폴리뉴클레오타이드 및 방법 |
| BR112021021075A2 (pt) | 2019-05-01 | 2021-12-14 | Editas Medicine Inc | Células que expressam um receptor recombinante de um locus de tgfbr2 modificado, polinucleotídeos relacionados e métodos |
| US11162079B2 (en) | 2019-05-10 | 2021-11-02 | The Regents Of The University Of California | Blood type O Rh-hypo-immunogenic pluripotent cells |
| CA3138329A1 (en) | 2019-05-10 | 2020-11-19 | Basf Se | Regulatory nucleic acid molecules for enhancing gene expression in plants |
| US20220249559A1 (en) | 2019-05-13 | 2022-08-11 | Iovance Biotherapeutics, Inc. | Methods and compositions for selecting tumor infiltrating lymphocytes and uses of the same in immunotherapy |
| EP3757219A1 (en) | 2019-06-28 | 2020-12-30 | KWS SAAT SE & Co. KGaA | Enhanced plant regeneration and transformation by using grf1 booster gene |
| WO2021003462A1 (en) | 2019-07-03 | 2021-01-07 | Factor Bioscience Inc. | Cationic lipids and uses thereof |
| EP4004216A1 (en) | 2019-07-25 | 2022-06-01 | Precision BioSciences, Inc. | Compositions and methods for sequential stacking of nucleic acid sequences into a genomic locus |
| US10501404B1 (en) | 2019-07-30 | 2019-12-10 | Factor Bioscience Inc. | Cationic lipids and transfection methods |
| JP2022543112A (ja) | 2019-08-01 | 2022-10-07 | サナ バイオテクノロジー,インコーポレイテッド | Dux4発現細胞およびそれらの使用 |
| WO2021035054A1 (en) | 2019-08-20 | 2021-02-25 | Precision Biosciences, Inc. | Lymphodepletion dosing regimens for cellular immunotherapies |
| WO2021035170A1 (en) | 2019-08-21 | 2021-02-25 | Precision Biosciences, Inc. | Compositions and methods for tcr reprogramming using fusion proteins |
| EP4017508A1 (en) | 2019-08-23 | 2022-06-29 | Sana Biotechnology, Inc. | Cd24 expressing cells and uses thereof |
| EP4028530A1 (en) | 2019-09-12 | 2022-07-20 | Basf Se | Regulatory nucleic acid molecules for enhancing gene expression in plants |
| WO2021069387A1 (en) | 2019-10-07 | 2021-04-15 | Basf Se | Regulatory nucleic acid molecules for enhancing gene expression in plants |
| US12435330B2 (en) | 2019-10-10 | 2025-10-07 | The Broad Institute, Inc. | Methods and compositions for prime editing RNA |
| WO2021081378A1 (en) | 2019-10-25 | 2021-04-29 | Iovance Biotherapeutics, Inc. | Gene editing of tumor infiltrating lymphocytes and uses of same in immunotherapy |
| WO2021087305A1 (en) | 2019-10-30 | 2021-05-06 | Precision Biosciences, Inc. | Cd20 chimeric antigen receptors and methods of use for immunotherapy |
| CA3157872A1 (en) | 2019-11-12 | 2021-05-20 | Otto Torjek | Gene for resistance to a pathogen of the genus heterodera |
| US12325861B2 (en) | 2019-12-03 | 2025-06-10 | Basf Se | Regulatory nucleic acid molecules for enhancing gene expression in plants |
| WO2021113543A1 (en) | 2019-12-06 | 2021-06-10 | Precision Biosciences, Inc. | Methods for cancer immunotherapy, using lymphodepletion regimens and cd19, cd20 or bcma allogeneic car t cells |
| WO2021118990A1 (en) | 2019-12-11 | 2021-06-17 | Iovance Biotherapeutics, Inc. | Processes for the production of tumor infiltrating lymphocytes (tils) and methods of using the same |
| CA3164591A1 (en) | 2020-01-13 | 2021-07-22 | William Dowdle | Modification of blood type antigens |
| KR20220142433A (ko) | 2020-01-17 | 2022-10-21 | 사나 바이오테크놀로지, 인크. | 유전자 발현 조절용 안전 스위치 |
| WO2021158915A1 (en) | 2020-02-06 | 2021-08-12 | Precision Biosciences, Inc. | Recombinant adeno-associated virus compositions and methods for producing and using the same |
| US12473563B2 (en) | 2020-02-28 | 2025-11-18 | KWS SAAT SE & Co. KGaA | Immature inflorescence meristem editing |
| MX2022011831A (es) | 2020-03-25 | 2023-01-04 | Sana Biotechnology Inc | Células neurales hipoinmunogénicas para el tratamiento de trastornos y afecciones neurológicas. |
| US20230263121A1 (en) | 2020-03-31 | 2023-08-24 | Elo Life Systems | Modulation of endogenous mogroside pathway genes in watermelon and other cucurbits |
| JP2023523855A (ja) | 2020-05-04 | 2023-06-07 | アイオバンス バイオセラピューティクス,インコーポレイテッド | 腫瘍浸潤リンパ球の製造方法及び免疫療法におけるその使用 |
| JP2023525304A (ja) | 2020-05-08 | 2023-06-15 | ザ ブロード インスティテュート,インコーポレーテッド | 標的二本鎖ヌクレオチド配列の両鎖同時編集のための方法および組成物 |
| WO2021231259A1 (en) | 2020-05-11 | 2021-11-18 | Precision Biosciences, Inc. | Self-limiting viral vectors encoding nucleases |
| WO2021231549A2 (en) * | 2020-05-12 | 2021-11-18 | Factor Bioscience Inc. | Engineered gene-editing proteins |
| EP4150057A2 (en) | 2020-05-13 | 2023-03-22 | Juno Therapeutics, Inc. | Process for producing donor-batched cells expressing a recombinant receptor |
| EP4171616A1 (en) | 2020-06-26 | 2023-05-03 | Juno Therapeutics GmbH | Engineered t cells conditionally expressing a recombinant receptor, related polynucleotides and methods |
| EP4192875A1 (en) | 2020-08-10 | 2023-06-14 | Precision BioSciences, Inc. | Antibodies and fragments specific for b-cell maturation antigen and uses thereof |
| MX2023001831A (es) | 2020-08-13 | 2023-06-29 | Sana Biotechnology Inc | Métodos de tratamiento de pacientes sensibilizados con células hipoinmunogénicas y métodos y composiciones asociados. |
| JP2023546359A (ja) | 2020-10-06 | 2023-11-02 | アイオバンス バイオセラピューティクス,インコーポレイテッド | 腫瘍浸潤リンパ球療法によるnsclc患者の治療 |
| WO2022076606A1 (en) | 2020-10-06 | 2022-04-14 | Iovance Biotherapeutics, Inc. | Treatment of nsclc patients with tumor infiltrating lymphocyte therapies |
| US20230365995A1 (en) | 2020-10-07 | 2023-11-16 | Precision Biosciences, Inc. | Lipid nanoparticle compositions |
| US20240060079A1 (en) | 2020-10-23 | 2024-02-22 | Elo Life Systems | Methods for producing vanilla plants with improved flavor and agronomic production |
| KR20230090367A (ko) | 2020-11-04 | 2023-06-21 | 주노 쎄러퓨티크스 인코퍼레이티드 | 변형된 불변 cd3 이뮤노글로불린 슈퍼패밀리 쇄 유전자좌로부터 키메라 수용체를 발현하는 세포 및 관련 폴리뉴클레오티드 및 방법 |
| JP2023554395A (ja) | 2020-12-17 | 2023-12-27 | アイオバンス バイオセラピューティクス,インコーポレイテッド | Ctla-4及びpd-1阻害剤と併用した腫瘍浸潤リンパ球療法による治療 |
| JP2024500403A (ja) | 2020-12-17 | 2024-01-09 | アイオバンス バイオセラピューティクス,インコーポレイテッド | 腫瘍浸潤リンパ球によるがんの治療 |
| EP4019639A1 (en) | 2020-12-22 | 2022-06-29 | KWS SAAT SE & Co. KGaA | Promoting regeneration and transformation in beta vulgaris |
| EP4019638A1 (en) | 2020-12-22 | 2022-06-29 | KWS SAAT SE & Co. KGaA | Promoting regeneration and transformation in beta vulgaris |
| KR20230137900A (ko) | 2020-12-31 | 2023-10-05 | 사나 바이오테크놀로지, 인크. | Car-t 활성을 조정하기 위한 방법 및 조성물 |
| US20250127811A1 (en) | 2021-01-28 | 2025-04-24 | Precision Biosciences, Inc. | Modulation of tgf beta signaling in genetically-modified eukaryotic cells |
| WO2022165260A1 (en) | 2021-01-29 | 2022-08-04 | Iovance Biotherapeutics, Inc. | Methods of making modified tumor infiltrating lymphocytes and their use in adoptive cell therapy |
| TW202304480A (zh) | 2021-03-19 | 2023-02-01 | 美商艾歐凡斯生物治療公司 | 腫瘤浸潤淋巴球(til)中之與cd39/cd69選擇及基因剔除相關之til擴增之方法 |
| EP4313122A1 (en) | 2021-03-23 | 2024-02-07 | Iovance Biotherapeutics, Inc. | Cish gene editing of tumor infiltrating lymphocytes and uses of same in immunotherapy |
| CA3215830A1 (en) | 2021-04-19 | 2022-10-27 | Rafael CUBAS | Chimeric costimulatory receptors, chemokine receptors, and the use of same in cellular immunotherapies |
| WO2022226316A1 (en) | 2021-04-22 | 2022-10-27 | Precision Biosciences, Inc. | Compositions and methods for generating male sterile plants |
| EP4340850A1 (en) | 2021-05-17 | 2024-03-27 | Iovance Biotherapeutics, Inc. | Pd-1 gene-edited tumor infiltrating lymphocytes and uses of same in immunotherapy |
| KR20240013135A (ko) | 2021-05-27 | 2024-01-30 | 사나 바이오테크놀로지, 인크. | 조작된 hla-e 또는 hla-g를 포함하는 저면역원성 세포 |
| AU2022309875A1 (en) | 2021-07-14 | 2024-01-25 | Sana Biotechnology, Inc. | Altered expression of y chromosome-linked antigens in hypoimmunogenic cells |
| JP2024526898A (ja) | 2021-07-22 | 2024-07-19 | アイオバンス バイオセラピューティクス,インコーポレイテッド | 固形腫瘍断片の凍結保存のための方法 |
| WO2023009716A1 (en) | 2021-07-28 | 2023-02-02 | Iovance Biotherapeutics, Inc. | Treatment of cancer patients with tumor infiltrating lymphocyte therapies in combination with kras inhibitors |
| AU2022325232A1 (en) | 2021-08-11 | 2024-02-08 | Sana Biotechnology, Inc. | Genetically modified primary cells for allogeneic cell therapy |
| WO2023019225A2 (en) | 2021-08-11 | 2023-02-16 | Sana Biotechnology, Inc. | Genetically modified cells for allogeneic cell therapy to reduce instant blood mediated inflammatory reactions |
| EP4384598A1 (en) | 2021-08-11 | 2024-06-19 | Sana Biotechnology, Inc. | Genetically modified cells for allogeneic cell therapy to reduce complement-mediated inflammatory reactions |
| EP4384544A1 (en) | 2021-08-11 | 2024-06-19 | Sana Biotechnology, Inc. | Genetically modified cells for allogeneic cell therapy |
| CA3231018A1 (en) | 2021-09-09 | 2023-03-16 | Iovance Biotherapeutics, Inc. | Processes for generating til products using pd-1 talen knockdown |
| WO2023064872A1 (en) | 2021-10-14 | 2023-04-20 | Precision Biosciences, Inc. | Combinations of anti-bcma car t cells and gamma secretase inhibitors |
| EP4419111A1 (en) | 2021-10-19 | 2024-08-28 | Precision Biosciences, Inc. | Gene editing methods for treating alpha-1 antitrypsin (aat) deficiency |
| CN113980141B (zh) * | 2021-10-27 | 2022-11-29 | 湖北大学 | 基于大肠杆菌素e家族dna酶的蛋白质复合物及其在人工蛋白支架中的应用 |
| CA3235824A1 (en) | 2021-10-27 | 2023-05-04 | Frederick G. Vogt | Systems and methods for coordinating manufacturing of cells for patient-specific immunotherapy |
| WO2023081767A1 (en) | 2021-11-05 | 2023-05-11 | Precision Biosciences, Inc. | Methods for immunotherapy |
| WO2023081900A1 (en) | 2021-11-08 | 2023-05-11 | Juno Therapeutics, Inc. | Engineered t cells expressing a recombinant t cell receptor (tcr) and related systems and methods |
| US11753677B2 (en) | 2021-11-10 | 2023-09-12 | Encodia, Inc. | Methods for barcoding macromolecules in individual cells |
| WO2023091910A1 (en) | 2021-11-16 | 2023-05-25 | Precision Biosciences, Inc. | Methods for cancer immunotherapy |
| EP4437091A1 (en) | 2021-11-23 | 2024-10-02 | Cellectis SA | New tale protein scaffolds with improved on-target/off-target activity ratios |
| CA3246480A1 (en) | 2022-01-07 | 2023-07-13 | Prec Biosciences Inc | POLYNUCLEOTIDES OPTIMIZED FOR PROTEIN EXPRESSION |
| US20250099588A1 (en) | 2022-01-28 | 2025-03-27 | Iovance Biotherapeutics, Inc. | Cytokine associated tumor infiltrating lymphocytes compositions and methods |
| KR20240155390A (ko) | 2022-02-17 | 2024-10-28 | 사나 바이오테크놀로지, 인크. | 조작된 cd47 단백질 및 이의 용도 |
| US20250101382A1 (en) | 2022-03-11 | 2025-03-27 | Sana Biotechnology, Inc. | Genetically modified cells and compositions and uses thereof |
| WO2023183313A1 (en) | 2022-03-22 | 2023-09-28 | Sana Biotechnology, Inc. | Engineering cells with a transgene in b2m or ciita locus and associated compositions and methods |
| CA3247638A1 (en) | 2022-04-06 | 2023-10-12 | Maria Fardis | TREATMENT OF PATIENTS WITH NON-SMALL CELL LUNG CARCINOMA (NSCLC) WITH TUMOR-INFILTRATING LYMPHOCYTE THERAPIES |
| CA3248034A1 (en) | 2022-04-15 | 2023-10-19 | Iovance Biotherapeutics Inc | METHODS FOR EXPANSION OF TIL CELLS BY MEANS OF SPECIFIC CYTOKINE COMBINATIONS AND/OR AKT INHIBITOR TREATMENT |
| JP2025516551A (ja) | 2022-05-10 | 2025-05-30 | アイオバンス バイオセラピューティクス,インコーポレイテッド | Il-15rアゴニストと併用した腫瘍浸潤リンパ球療法によるがん患者の治療 |
| CA3267168A1 (en) | 2022-09-09 | 2024-03-14 | Rongsu Qi | PROCESSES FOR GENERING TIL PRODUCTS USING DOUBLE INACTIVATION OF TALEN PD-1/TIGIT |
| CA3267183A1 (en) | 2022-09-09 | 2024-03-14 | Hequn Yin | PROCESSES FOR GENERING TIL PRODUCTS USING DOUBLE TALEN INACTIVATION OF PD-1/TIGIT |
| EP4605524A1 (en) | 2022-10-20 | 2025-08-27 | Basf Se | Regulatory nucleic acid molecules for enhancing gene expression in plants |
| WO2024097313A1 (en) | 2022-11-02 | 2024-05-10 | Sana Biotechnology, Inc. | Methods for producing t cell therapy products |
| WO2024098027A1 (en) | 2022-11-04 | 2024-05-10 | Iovance Biotherapeutics, Inc. | Methods for tumor infiltrating lymphocyte (til) expansion related to cd39/cd103 selection |
| WO2024098024A1 (en) | 2022-11-04 | 2024-05-10 | Iovance Biotherapeutics, Inc. | Expansion of tumor infiltrating lymphocytes from liquid tumors and therapeutic uses thereof |
| WO2024100604A1 (en) | 2022-11-09 | 2024-05-16 | Juno Therapeutics Gmbh | Methods for manufacturing engineered immune cells |
| IL320733A (en) | 2022-11-21 | 2025-07-01 | Iovance Biotherapeutics Inc | Two-dimensional processes for expanding tumor-infiltrating lymphocytes and treatments thereof |
| WO2024112711A2 (en) | 2022-11-21 | 2024-05-30 | Iovance Biotherapeutics, Inc. | Methods for assessing proliferation potency of gene-edited t cells |
| WO2024118836A1 (en) | 2022-11-30 | 2024-06-06 | Iovance Biotherapeutics, Inc. | Processes for production of tumor infiltrating lymphocytes with shortened rep step |
| WO2024151541A1 (en) | 2023-01-09 | 2024-07-18 | Sana Biotechnology, Inc. | Type-1 diabetes autoimmune mouse |
| EP4658675A1 (en) | 2023-02-03 | 2025-12-10 | C3S2 GmbH | Methods for non-viral manufacturing of engineered immune cells |
| WO2024216116A1 (en) | 2023-04-14 | 2024-10-17 | Precision Biosciences, Inc. | Muscle-specific expression cassettes |
| AU2024255934A1 (en) | 2023-04-14 | 2025-11-20 | Precision Biosciences, Inc. | Muscle-specific expression cassettes |
| AU2024264889A1 (en) | 2023-05-03 | 2025-11-13 | Sana Biotechnology, Inc. | Methods of dosing and administration of engineered islet cells |
| WO2024243236A2 (en) | 2023-05-22 | 2024-11-28 | Sana Biotechnology, Inc. | Methods of delivery of islet cells and related methods |
| WO2025043172A1 (en) | 2023-08-23 | 2025-02-27 | Sana Biotechnology, Inc. | Modified cd47 proteins and their uses |
| WO2025054540A1 (en) | 2023-09-08 | 2025-03-13 | Iovance Biotherapeutics, Inc. | Methods of gene-editing using programmable nucleases |
| CN117344048A (zh) * | 2023-09-28 | 2024-01-05 | 江苏省农业科学院 | 检测无毒基因Avr-Pik的RPA-LFD引物探针组、试剂盒及其应用 |
| WO2025101484A1 (en) | 2023-11-06 | 2025-05-15 | Iovance Biotherapeutics, Inc. | Treatment of endometrial cancers with tumor infiltrating lymphocyte therapies |
| CN117821413B (zh) * | 2023-12-15 | 2024-12-27 | 湖北大学 | 一种高进行性Pfu DNA聚合酶及其制备方法和应用 |
| WO2025137646A1 (en) | 2023-12-22 | 2025-06-26 | Recode Therapeutics, Inc. | Gene editing methods and compositions for treating cystic fibrosis |
| WO2025181336A1 (en) | 2024-03-01 | 2025-09-04 | Cellectis Sa | Compositions and methods for hbb-editing in hspc |
| WO2025212519A1 (en) | 2024-04-01 | 2025-10-09 | Moonlight Bio, Inc. | Dll3 binding proteins and uses thereof |
| US20250345431A1 (en) | 2024-05-10 | 2025-11-13 | Juno Therapeutics, Inc. | Genetically engineered t cells expressing a cd19 chimeric antigen receptor (car) and uses thereof for allogeneic cell therapy |
Family Cites Families (30)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4179337A (en) | 1973-07-20 | 1979-12-18 | Davis Frank F | Non-immunogenic polypeptides |
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US5006333A (en) | 1987-08-03 | 1991-04-09 | Ddi Pharmaceuticals, Inc. | Conjugates of superoxide dismutase coupled to high molecular weight polyalkylene glycols |
| WO2009095742A1 (en) | 2008-01-31 | 2009-08-06 | Cellectis | New i-crei derived single-chain meganuclease and uses thereof |
| WO2003078619A1 (en) | 2002-03-15 | 2003-09-25 | Cellectis | Hybrid and single chain meganucleases and use thereof |
| WO2004031346A2 (en) | 2002-09-06 | 2004-04-15 | Fred Hutchinson Cancer Research Center | Methods and compositions concerning designed highly-specific nucleic acid binding proteins |
| EP1590468A2 (en) | 2003-01-28 | 2005-11-02 | Cellectis | Use of meganucleases for inducing homologous recombination ex vivo and in toto in vertebrate somatic tissues and application thereof |
| WO2007034262A1 (en) | 2005-09-19 | 2007-03-29 | Cellectis | Heterodimeric meganucleases and use thereof |
| WO2006097784A1 (en) | 2005-03-15 | 2006-09-21 | Cellectis | I-crei meganuclease variants with modified specificity, method of preparation and uses thereof |
| WO2006097854A1 (en) | 2005-03-15 | 2006-09-21 | Cellectis | Heterodimeric meganucleases and use thereof |
| WO2007049095A1 (en) | 2005-10-25 | 2007-05-03 | Cellectis | Laglidadg homing endonuclease variants having mutations in two functional subdomains and use thereof |
| WO2007060495A1 (en) | 2005-10-25 | 2007-05-31 | Cellectis | I-crei homing endonuclease variants having novel cleavage specificity and use thereof |
| WO2007093836A1 (en) | 2006-02-13 | 2007-08-23 | Cellectis | Meganuclease variants cleaving a dna target sequence from a xp gene and uses thereof |
| WO2008010009A1 (en) | 2006-07-18 | 2008-01-24 | Cellectis | Meganuclease variants cleaving a dna target sequence from a rag gene and uses thereof |
| BRPI0718747A2 (pt) | 2006-11-14 | 2013-12-03 | Cellectis | Variantes meganuclease que clavam uma ou sequência alvo de dna a partir do gene hprt e usos das mesmas. |
| WO2008093152A1 (en) | 2007-02-01 | 2008-08-07 | Cellectis | Obligate heterodimer meganucleases and uses thereof |
| WO2008102199A1 (en) | 2007-02-20 | 2008-08-28 | Cellectis | Meganuclease variants cleaving a dna target sequence from the beta-2-microglobulin gene and uses thereof |
| WO2008149176A1 (en) | 2007-06-06 | 2008-12-11 | Cellectis | Meganuclease variants cleaving a dna target sequence from the mouse rosa26 locus and uses thereof |
| WO2009013559A1 (en) | 2007-07-23 | 2009-01-29 | Cellectis | Meganuclease variants cleaving a dna target sequence from the human hemoglobin beta gene and uses thereof |
| WO2009019528A1 (en) | 2007-08-03 | 2009-02-12 | Cellectis | Meganuclease variants cleaving a dna target sequence from the human interleukin-2 receptor gamma chain gene and uses thereof |
| EP2206723A1 (en) * | 2009-01-12 | 2010-07-14 | Bonas, Ulla | Modular DNA-binding domains |
| US8956828B2 (en) * | 2009-11-10 | 2015-02-17 | Sangamo Biosciences, Inc. | Targeted disruption of T cell receptor genes using engineered zinc finger protein nucleases |
| CN102762726A (zh) * | 2009-11-27 | 2012-10-31 | 巴斯夫植物科学有限公司 | 嵌合内切核酸酶及其用途 |
| CA2783351C (en) * | 2009-12-10 | 2021-09-07 | Regents Of The University Of Minnesota | Tal effector-mediated dna modification |
| CN103097527A (zh) * | 2010-02-26 | 2013-05-08 | 塞勒克提斯公司 | 用于将转基因插入安全港座位的内切核酸酶的用途 |
| CA2798988C (en) * | 2010-05-17 | 2020-03-10 | Sangamo Biosciences, Inc. | Tal-effector (tale) dna-binding polypeptides and uses thereof |
| JP2013544082A (ja) * | 2010-10-27 | 2013-12-12 | セレクティス | 二本鎖破断−誘導変異誘発の効率を増大させる方法 |
| US9499592B2 (en) * | 2011-01-26 | 2016-11-22 | President And Fellows Of Harvard College | Transcription activator-like effectors |
| EP4062927A1 (en) * | 2011-02-28 | 2022-09-28 | Seattle Children's Research Institute | Coupling endonucleases with end-processing enzymes drives high efficiency gene disruption |
| AU2014273091B2 (en) * | 2013-05-31 | 2019-12-12 | Cellectis | A LAGLIDADG homing endonuclease cleaving the T cell receptor alpha gene and uses thereof |
-
2012
- 2012-04-05 EP EP12725896.0A patent/EP2694091B1/en active Active
- 2012-04-05 EP EP17195812.7A patent/EP3320910A1/en active Pending
- 2012-04-05 SG SG2013074745A patent/SG194115A1/en unknown
- 2012-04-05 CA CA3111953A patent/CA3111953C/en active Active
- 2012-04-05 CA CA2832534A patent/CA2832534C/en active Active
- 2012-04-05 ES ES12725896T patent/ES2728436T3/es active Active
- 2012-04-05 US US14/009,509 patent/US20140115726A1/en not_active Abandoned
- 2012-04-05 AU AU2012240110A patent/AU2012240110B2/en active Active
- 2012-04-05 WO PCT/US2012/032439 patent/WO2012138939A1/en not_active Ceased
- 2012-04-05 SG SG10201602651YA patent/SG10201602651YA/en unknown
- 2012-04-05 JP JP2014504007A patent/JP5996630B2/ja active Active
- 2012-04-05 KR KR1020137029369A patent/KR101982360B1/ko active Active
- 2012-04-05 DK DK12725896.0T patent/DK2694091T3/da active
- 2012-04-05 CN CN201280027394.9A patent/CN103608027B/zh active Active
- 2012-04-05 EP EP12715261.9A patent/EP2694089B1/en active Active
- 2012-04-05 WO PCT/US2012/032426 patent/WO2012138927A2/en not_active Ceased
- 2012-04-05 US US13/440,940 patent/US9315788B2/en active Active
-
2013
- 2013-10-06 IL IL228747A patent/IL228747B/en active IP Right Grant
-
2014
- 2014-03-19 HK HK18113103.7A patent/HK1253952A1/en unknown
-
2016
- 2016-03-14 US US15/069,672 patent/US11198856B2/en active Active
- 2016-08-24 JP JP2016163762A patent/JP6695239B2/ja active Active
-
2017
- 2017-10-17 AU AU2017248447A patent/AU2017248447B2/en active Active
-
2018
- 2018-09-28 JP JP2018184887A patent/JP6839691B2/ja active Active
-
2019
- 2019-01-02 US US16/237,901 patent/US11136566B2/en active Active
-
2021
- 2021-09-29 US US17/489,454 patent/US20220010292A1/en not_active Abandoned
Also Published As
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US20220010292A1 (en) | Method for the generation of compact tale-nucleases and uses thereof | |
| US9540623B2 (en) | Method for increasing the efficiency of double-strand-break induced mutagenesis | |
| EP2633040B1 (en) | Method for increasing the efficiency of double-strand break-induced mutagenesis | |
| US11220683B2 (en) | Method to overcome DNA chemical modifications sensitivity of engineered TALE DNA binding domains | |
| US20150067900A1 (en) | New repeat variable diresidues for targeting nucleotides | |
| US20170260513A1 (en) | Tevi chimeric endonuclease and their preferential cleavage sites | |
| WO2012138901A1 (en) | Method for enhancing rare-cutting endonuclease efficiency and uses thereof | |
| HK1192709A (en) | Method for the generation of compact tale-nucleases and uses thereof | |
| HK1192709B (en) | Method for the generation of compact tale-nucleases and uses thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request |
Effective date: 20210525 |
|
| EEER | Examination request |
Effective date: 20210525 |
|
| EEER | Examination request |
Effective date: 20210525 |
|
| EEER | Examination request |
Effective date: 20210525 |
|
| EEER | Examination request |
Effective date: 20210525 |
|
| EEER | Examination request |
Effective date: 20210525 |
|
| EEER | Examination request |
Effective date: 20210525 |
|
| EEER | Examination request |
Effective date: 20210525 |
|
| EEER | Examination request |
Effective date: 20210525 |