CA2443908C - Product and process for transformation of thraustochytriales microorganisms - Google Patents

Product and process for transformation of thraustochytriales microorganisms Download PDF

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Publication number
CA2443908C
CA2443908C CA2443908A CA2443908A CA2443908C CA 2443908 C CA2443908 C CA 2443908C CA 2443908 A CA2443908 A CA 2443908A CA 2443908 A CA2443908 A CA 2443908A CA 2443908 C CA2443908 C CA 2443908C
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Canada
Prior art keywords
nucleic acid
acid sequence
seq
thraustochytriales
protein
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Expired - Fee Related
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CA2443908A
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English (en)
French (fr)
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CA2443908A1 (en
Inventor
Paul G. Roessler
T. Dave Matthews
Tom M. Ramseier
James G. Metz
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DSM IP Assets BV
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DSM IP Assets BV
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Publication of CA2443908C publication Critical patent/CA2443908C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y202/00—Transferases transferring aldehyde or ketonic groups (2.2)
    • C12Y202/01—Transketolases and transaldolases (2.2.1)
    • C12Y202/01006—Acetolactate synthase (2.2.1.6)

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Mushroom Cultivation (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Enzymes And Modification Thereof (AREA)
CA2443908A 2001-04-16 2002-04-16 Product and process for transformation of thraustochytriales microorganisms Expired - Fee Related CA2443908C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US28411601P 2001-04-16 2001-04-16
US60/284,116 2001-04-16
PCT/US2002/012040 WO2002083869A2 (en) 2001-04-16 2002-04-16 Product and process for transformation of thraustochytriales microorganisms

Publications (2)

Publication Number Publication Date
CA2443908A1 CA2443908A1 (en) 2002-10-24
CA2443908C true CA2443908C (en) 2016-01-12

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
CA2443908A Expired - Fee Related CA2443908C (en) 2001-04-16 2002-04-16 Product and process for transformation of thraustochytriales microorganisms

Country Status (10)

Country Link
US (5) US7001772B2 (https=)
EP (3) EP2325319B1 (https=)
JP (4) JP4536324B2 (https=)
CN (3) CN101760469A (https=)
AT (1) ATE533844T1 (https=)
AU (1) AU2002250592C1 (https=)
CA (1) CA2443908C (https=)
ES (3) ES2377557T3 (https=)
TW (3) TWI377253B (https=)
WO (1) WO2002083869A2 (https=)

Families Citing this family (80)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6566583B1 (en) * 1997-06-04 2003-05-20 Daniel Facciotti Schizochytrium PKS genes
US20070244192A1 (en) * 1999-01-14 2007-10-18 Martek Biosciences Corporation Plant seed oils containing polyunsaturated fatty acids
US7211418B2 (en) * 1999-01-14 2007-05-01 Martek Biosciences Corporation PUFA polyketide synthase systems and uses thereof
US7217856B2 (en) * 1999-01-14 2007-05-15 Martek Biosciences Corporation PUFA polyketide synthase systems and uses thereof
US7247461B2 (en) * 1999-01-14 2007-07-24 Martek Biosciences Corporation Nucleic acid molecule encoding ORFA of a PUFA polyketide synthase system and uses thereof
US8003772B2 (en) * 1999-01-14 2011-08-23 Martek Biosciences Corporation Chimeric PUFA polyketide synthase systems and uses thereof
TWI377253B (en) * 2001-04-16 2012-11-21 Martek Biosciences Corp Product and process for transformation of thraustochytriales microorganisms
US7211656B2 (en) * 2002-01-30 2007-05-01 Abbott Laboratories Desaturase genes, enzymes encoded thereby, and uses thereof
ATE354645T1 (de) * 2002-12-20 2007-03-15 Chromagenics Bv Mittel und methoden zur produktion eines proteins durch chromatin-öffner, die chromatin zugänglicher für transkriptionsfaktoren machen können
US7208590B2 (en) * 2003-07-15 2007-04-24 Abbott Laboratories Genes involved in polyketide synthase pathways and uses thereof
CN103451246B (zh) 2004-04-22 2018-02-16 联邦科学技术研究组织 用重组细胞合成长链多不饱和脂肪酸
EP2357244A3 (en) 2004-04-22 2011-11-23 Commonwealth Scientific and Industrial Research Organisation Synthesis of long-chain polyunsaturated fatty acids by recombinant cells.
EP1809750B1 (en) * 2004-11-08 2012-03-21 ChromaGenics B.V. Selection of host cells expressing protein at high levels
US8999667B2 (en) * 2004-11-08 2015-04-07 Chromagenics B.V. Selection of host cells expressing protein at high levels
US20060195935A1 (en) 2004-11-08 2006-08-31 Chromagenics B.V. Selection of host cells expressing protein at high levels
US20100136616A1 (en) * 2004-11-08 2010-06-03 Chromagenics B.V. Selection of Host Cells Expressing Protein at High Levels
US20060172382A1 (en) * 2004-11-08 2006-08-03 Chromagenics B.V. Selection of host cells expressing protein at high levels
JP4796787B2 (ja) 2005-04-28 2011-10-19 富士フイルム株式会社 ラビリンチュラ類への遺伝子導入法
JP4796786B2 (ja) * 2005-04-28 2011-10-19 富士フイルム株式会社 ラビリンチュラ類を形質転換可能なベクター
AU2006325040B2 (en) 2005-06-07 2012-04-19 Dsm Nutritional Products Ag Eukaryotic microorganisms for producing lipids and antioxidants
AU2006316610B2 (en) 2005-11-23 2012-06-14 E. I. Du Pont De Nemours And Company Delta-9 elongases and their use in making polyunsaturated fatty acids
EP1818411A1 (en) * 2006-02-13 2007-08-15 Lonza AG Process for the preparation of optically active chiral amines
WO2007106905A2 (en) * 2006-03-15 2007-09-20 Martek Biosciences Corporation Polyunsaturated fatty acid production in heterologous organisms using pufa polyketide synthase systems
KR20090007407A (ko) * 2006-04-11 2009-01-16 마텍 바이오싸이언스스 코포레이션 장쇄 다중불포화 지방산을 포함하는 식품 제품 및 그의 제조 방법
EP2034973B2 (en) 2006-06-22 2022-01-19 DSM IP Assets B.V. Encapsulated labile compound compositions and methods of making the same
CA2659603C (en) * 2006-08-01 2017-03-07 Ocean Nutrition Canada Limited Oil producing microbes and methods of modification thereof
RU2009111266A (ru) 2006-08-29 2010-10-10 Коммонвелт Сайентифик энд Индастриал Рисерч Организейшн (AU) Синтез жирных кислот
EP2415360A3 (en) 2006-08-29 2012-02-29 Martek Biosciences Corporation Use of DPA(n-6) oils in infant formula
KR100860111B1 (ko) * 2006-10-02 2008-09-25 인제대학교 산학협력단 신규 미생물 트라우스토카이트리움 에스피.케이제이에스-1, 바실러스 폴리퍼멘티쿠스 케이제이에스-2 및 이들과 바실러스 리케니포르미스를 포함하고 있는 사료첨가제 및 사료
EP2082050B1 (en) 2006-10-23 2013-04-24 E.I. Du Pont De Nemours And Company Delta-8 desaturases and their use in making polyunsaturated fatty acids
US8188338B2 (en) 2007-04-10 2012-05-29 E. I. Du Pont De Nemours And Company Delta-8 desaturases and their use in making polyunsaturated fatty acids
US8119860B2 (en) 2007-04-16 2012-02-21 E. I. Du Pont De Nemours And Company Delta-9 elongases and their use in making polyunsaturated fatty acids
US8957280B2 (en) 2007-05-03 2015-02-17 E. I. Du Pont De Nemours And Company Delta-5 desaturases and their use in making polyunsaturated fatty acids
AU2008269989B2 (en) * 2007-06-29 2014-02-27 Dsm Ip Assets B.V. Production and purification of esters of polyunsaturated fatty acids
US20090064567A1 (en) 2007-09-12 2009-03-12 Martek Biosciences Corporation Biological oils and production and uses Thereof
US20110076379A1 (en) * 2008-06-13 2011-03-31 Means Michael M High omega saturated fat free meat products
BRPI0921467B1 (pt) 2008-11-18 2021-12-14 Commonwealth Scientific And Industrial Research Organisation Processo para a produção de óleo compreendendo ácido eicosapentaenóico (epa), ácido docosapentaenóico (dpa) e ácido docosahexaenóico (dha)
AU2009335903B2 (en) 2008-12-18 2016-04-14 E. I. Du Pont De Nemours And Company Reducing byproduction of malonates in a fermentation process
JP5415896B2 (ja) 2009-01-29 2014-02-12 Ｊｆｅスチール株式会社 インダイレクトスポット溶接方法
ES2752196T3 (es) * 2009-03-16 2020-04-03 Dsm Ip Assets Bv Producción de proteínas en microorganismos del filo Labyrinthulomycota
US8207363B2 (en) 2009-03-19 2012-06-26 Martek Biosciences Corporation Thraustochytrids, fatty acid compositions, and methods of making and uses thereof
US8940884B2 (en) 2009-03-19 2015-01-27 Dsm Ip Assets B.V. Polyunsaturated fatty acid synthase nucleic acid molecules and polypeptides, compositions, and methods of making and uses thereof
CA2763424C (en) 2009-06-16 2018-06-12 E.I. Du Pont De Nemours And Company Improvement of long chain omega-3 and omega-6 polyunsaturated fatty acid biosynthesis by expression of acyl-coa lysophospholipid acyltransferases
WO2011037207A1 (ja) * 2009-09-24 2011-03-31 国立大学法人九州大学 ストラメノパイルの形質転換方法
CN103068965A (zh) 2009-12-28 2013-04-24 Dsmip资产公司 在蔗糖上生长的重组破囊壶菌和其组合物、制备方法及用途
CN103260643A (zh) * 2009-12-28 2013-08-21 梅里亚有限公司 重组ndv抗原及其用途
EP2519636B8 (en) * 2009-12-28 2017-08-09 DSM IP Assets B.V. Production of hemagglutinin-neuraminidase protein in microalgae
ES2651313T3 (es) * 2009-12-28 2018-01-25 Dsm Ip Assets B.V. Traustoquítridos recombinantes que crecen en xilosa, y composiciones, métodos de preparación y usos de los mismos
IN2012DN06277A (https=) * 2009-12-28 2015-09-25 Dsm Ip Assets Bv
US9924733B2 (en) * 2010-01-19 2018-03-27 Dsm Ip Assets B.V. Eicosapentaenoic acid-producing microorganisms, fatty acid compositions, and methods of making and uses thereof
ES2617743T3 (es) 2010-03-12 2017-06-19 Merial, Inc. Vacunas recombinantes del virus de la lengua azul y sus utilizaciones
KR20140008295A (ko) 2010-08-26 2014-01-21 이 아이 듀폰 디 네모아 앤드 캄파니 돌연변이 델타-9 연장효소 및 다중불포화 지방산의 제조에서의 그들의 용도
JP2013535988A (ja) 2010-08-26 2013-09-19 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー 変異ｈｐｇｇモチーフおよびｈｄａｓｈモチーフδ５デサチュラーゼおよび多価不飽和脂肪酸の製造におけるそれらの使用
PT2683824T (pt) * 2011-03-07 2018-06-07 Dsm Nutritional Products Ag Modificação de microorganismos thraustochytrid
AU2012285806B2 (en) 2011-07-21 2017-09-14 Dsm Ip Assets B.V. Fatty acid compositions
US8844424B2 (en) * 2011-08-17 2014-09-30 Lwrc International Llc Bolt carrier and bolt for gas operated firearms
US8950312B2 (en) * 2011-08-17 2015-02-10 Lwrc International Llc Bolt carrier and bolt for gas operated firearms
WO2013185184A2 (en) 2012-06-15 2013-12-19 Commonwealth Scientific And Industrial Research Organisation Production of long chain polyunsaturated fatty acids in plant cells
CA2877334A1 (en) 2012-06-19 2013-12-27 E. I. Du Pont De Nemours And Company Mutant acyl-coa:lysophosphatidylcholine acyltransferases
EP2970926B1 (en) 2013-03-13 2018-01-31 DSM Nutritional Products AG Engineering microorganisms
EP3082405A4 (en) 2013-12-18 2017-12-13 Commonwealth Scientific and Industrial Research Organisation Lipid comprising long chain polyunsaturated fatty acids
SG11201610596PA (en) 2014-06-27 2017-01-27 Commw Scient Ind Res Org Lipid comprising docosapentaenoic acid
CN105567715B (zh) * 2014-10-17 2021-06-29 丰益(上海)生物技术研发中心有限公司 裂殖壶菌α-微管蛋白相关序列及其应用
CA2977271A1 (en) 2015-03-02 2016-09-09 Synthetic Genomics, Inc. Regulatory elements from labyrinthulomycetes microorganisms
AR104042A1 (es) 2015-03-26 2017-06-21 Mara Renewables Corp Producción de alta densidad de biomasa y aceite utilizando glicerol en bruto
CN107846857B (zh) 2015-06-06 2021-07-02 帝斯曼知识产权资产管理有限公司 使用新模块二十二碳六烯酸(dha)合酶生成多不饱和脂肪酸(pufas)
US10542961B2 (en) 2015-06-15 2020-01-28 The Research Foundation For The State University Of New York System and method for infrasonic cardiac monitoring
EP3322796A4 (en) 2015-07-13 2019-03-27 Mara Renewables Corporation IMPROVEMENT OF MICROGENONAL FLUID CHANGES OF XYLOSE
IL241462A0 (en) 2015-09-10 2015-11-30 Yeda Res & Dev Heterologous engineering of betalain pigments in plants
EP3795586A3 (en) 2016-03-16 2021-06-23 Conagen Inc. Production of proteins in labyrinthulomycetes
PL3455351T3 (pl) * 2016-05-12 2022-11-21 Dsm Ip Assets B.V. Sposób zwiększania wytwarzania wielonienasyconych kwasów tłuszczowych omega-3 w mikroalgach
US10851395B2 (en) 2016-06-10 2020-12-01 MARA Renewables Corporation Method of making lipids with improved cold flow properties
KR102319845B1 (ko) 2016-07-13 2021-11-01 디에스엠 아이피 어셋츠 비.브이. 조류 숙주 세포에 대한 crispr-cas 시스템
IL247752A0 (en) 2016-09-11 2016-11-30 Yeda Res & Dev Compositions and methods for modulating gene expression for site-directed mutagenesis
US10633454B2 (en) 2016-11-01 2020-04-28 Conagen Inc. Expression of modified glycoproteins and glycopeptides
WO2019078359A1 (ja) 2017-10-19 2019-04-25 味の素株式会社 ステロールの製造法
EP3762485A4 (en) * 2018-03-05 2021-12-08 Conagen Inc. ORGANISMS AND METHOD FOR MANUFACTURING GLYCOMOLECULES WITH LOW SULFATION
CN112074608A (zh) * 2018-05-01 2020-12-11 科纳根公司 用于产生具有高聚糖占有率的糖分子的重组生物体和方法
BR112021002300A2 (pt) * 2018-08-10 2021-05-04 Kyowa Hakko Bio Co., Ltd microrganismo que produz ácido eicosapentaenoico e método para a produção de ácido eicosapentaenoico
CN109777815B (zh) * 2019-03-28 2021-10-29 昆明理工大学 HMG-CoA合成酶基因RKHMGCS及其应用

Family Cites Families (49)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BE545283A (https=)
LU35839A1 (https=)
US3223456A (en) * 1963-11-14 1965-12-14 Siemens Ag Conveying apparatus for fine-granular material
US4381897A (en) * 1980-10-06 1983-05-03 Krupp Polysius Ag Installation for transporting fine-grained material
US4626505A (en) 1984-02-24 1986-12-02 E. I. Du Pont De Nemours And Company Selectable markers for yeast transformation
IL83348A (en) * 1986-08-26 1995-12-08 Du Pont Nucleic acid fragment encoding herbicide resistant plant acetolactate synthase
US5605011A (en) * 1986-08-26 1997-02-25 E. I. Du Pont De Nemours And Company Nucleic acid fragment encoding herbicide resistant plant acetolactate synthase
US5130242A (en) * 1988-09-07 1992-07-14 Phycotech, Inc. Process for the heterotrophic production of microbial products with high concentrations of omega-3 highly unsaturated fatty acids
US5340742A (en) 1988-09-07 1994-08-23 Omegatech Inc. Process for growing thraustochytrium and schizochytrium using non-chloride salts to produce a microfloral biomass having omega-3-highly unsaturated fatty acids
US5426040A (en) * 1989-08-17 1995-06-20 Northeastern University Methods for producing improved strains of seaweed by fusion of spore-protoplasts, and resultant seaweeds and phycocolloids
IL100908A0 (en) 1991-02-22 1992-11-15 Salk Inst Biotech Ind Invertase genes and their use
US5804408A (en) 1991-03-13 1998-09-08 Yoshihide Hagiwara Expression of human SOD in blue green algae
US5661017A (en) 1993-09-14 1997-08-26 Dunahay; Terri Goodman Method to transform algae, materials therefor, and products produced thereby
IL113375A (en) * 1995-04-13 1997-09-30 Fortress U & T Ltd Internationally regulated system for one to one cryptographic communications with national sovereignty without key escrow
US5853973A (en) * 1995-04-20 1998-12-29 American Cyanamid Company Structure based designed herbicide resistant products
CA2218526C (en) * 1995-04-20 2012-06-12 American Cyanamid Company Structure-based designed herbicide resistant products
DE19529475A1 (de) 1995-08-11 1997-02-13 Marcus Weis Siebkonstruktion zum Abtrennen des Faseranteils aus der Shredderleichtfraktion
US5824309A (en) 1995-12-05 1998-10-20 University Of Massachusetts Recombinant gas vesicles and uses thereof
WO1997039106A1 (en) 1996-04-12 1997-10-23 Martek Biosciences Corporation Methods and tools for transformation of eukaryotic algae
US6194167B1 (en) * 1997-02-18 2001-02-27 Washington State University Research Foundation ω-3 fatty acid desaturase
US5968809A (en) * 1997-04-11 1999-10-19 Abbot Laboratories Methods and compositions for synthesis of long chain poly-unsaturated fatty acids
ES2125195B1 (es) 1997-04-18 1999-10-01 Antibioticos Sa Procedimiento de inactivacion de genes que codifican para enzimas del catabolismo del fenilacetato, plasmidos que intervienen y cepas transformadas con los mismos.
US8003772B2 (en) 1999-01-14 2011-08-23 Martek Biosciences Corporation Chimeric PUFA polyketide synthase systems and uses thereof
US7247461B2 (en) * 1999-01-14 2007-07-24 Martek Biosciences Corporation Nucleic acid molecule encoding ORFA of a PUFA polyketide synthase system and uses thereof
FR2791699B1 (fr) * 1999-03-29 2004-10-29 Meristem Therapeutics Promoteurs chimeriques d'expression, cassettes d'expression, vecteurs, plantes et semences transgeniques les contenant, et methodes de leur obtention
KR20000075076A (ko) * 1999-05-28 2000-12-15 최태진 형질전환된 미세조류를 이용하여 외래 단백질을 생산하는 방법
US7795002B2 (en) 2000-06-28 2010-09-14 Glycofi, Inc. Production of galactosylated glycoproteins in lower eukaryotes
EP2322644A1 (en) 2000-06-28 2011-05-18 GlycoFi, Inc. Methods for producing modified glycoproteins
US20060257399A1 (en) 2000-06-28 2006-11-16 Glycofi, Inc. Immunoglobulins comprising predominantly a Man5GIcNAc2 glycoform
US20060029604A1 (en) 2000-06-28 2006-02-09 Gerngross Tillman U Immunoglobulins comprising predominantly a GlcNAc2Man3GlcNAc2 glycoform
US7625756B2 (en) 2000-06-28 2009-12-01 GycoFi, Inc. Expression of class 2 mannosidase and class III mannosidase in lower eukaryotic cells
EP1911837B1 (en) * 2000-09-28 2011-05-25 Bioriginal Food & Science Corp. FAD5-2 fatty acid desaturase family member and uses thereof
US6419425B1 (en) * 2001-02-28 2002-07-16 Neu Transf'air Granular material distributing apparatus comprising at least two transfer vessels that operate in alternation
TWI377253B (en) * 2001-04-16 2012-11-21 Martek Biosciences Corp Product and process for transformation of thraustochytriales microorganisms
US7332299B2 (en) 2003-02-20 2008-02-19 Glycofi, Inc. Endomannosidases in the modification of glycoproteins in eukaryotes
ATE404582T1 (de) 2003-06-05 2008-08-15 Wyeth Corp Immunogene zusammensetzungen, die replikonvektoren des venezolanischen pferdeencephalitisvirus und paramyxovirus- proteinantigene enthalten
JP4796787B2 (ja) 2005-04-28 2011-10-19 富士フイルム株式会社 ラビリンチュラ類への遺伝子導入法
JP4796786B2 (ja) 2005-04-28 2011-10-19 富士フイルム株式会社 ラビリンチュラ類を形質転換可能なベクター
PT103331A (pt) 2005-08-05 2007-02-28 Fundacao Da Faculdade De Cienc Expressão dum transportador activo de xilose em saccharomyces cerevisiae modificada geneticamente
WO2007106905A2 (en) 2006-03-15 2007-09-20 Martek Biosciences Corporation Polyunsaturated fatty acid production in heterologous organisms using pufa polyketide synthase systems
CA2659603C (en) 2006-08-01 2017-03-07 Ocean Nutrition Canada Limited Oil producing microbes and methods of modification thereof
CA2615372A1 (en) 2007-07-13 2009-01-13 Marc-Andre D'aoust Influenza virus-like particles (vlps) comprising hemagglutinin
US20090064567A1 (en) 2007-09-12 2009-03-12 Martek Biosciences Corporation Biological oils and production and uses Thereof
EP2090648A1 (en) 2008-02-12 2009-08-19 Institut Francais de Recherche pour l'Exploitation de la Mere(Ifremer) Production of glycosylated polypeptides in microalgae
CN102057048B (zh) 2008-04-09 2015-01-21 索拉兹米公司 微生物生物质和微生物油的直接化学修饰
EP2519636B8 (en) 2009-12-28 2017-08-09 DSM IP Assets B.V. Production of hemagglutinin-neuraminidase protein in microalgae
ES2651313T3 (es) 2009-12-28 2018-01-25 Dsm Ip Assets B.V. Traustoquítridos recombinantes que crecen en xilosa, y composiciones, métodos de preparación y usos de los mismos
IN2012DN06277A (https=) 2009-12-28 2015-09-25 Dsm Ip Assets Bv
CN103068965A (zh) 2009-12-28 2013-04-24 Dsmip资产公司 在蔗糖上生长的重组破囊壶菌和其组合物、制备方法及用途

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