CA2252412A1 - Utilisation de la proteine fluorescente verte comme marqueur de criblage pour la transformation de plantes - Google Patents

Utilisation de la proteine fluorescente verte comme marqueur de criblage pour la transformation de plantes

Info

Publication number
CA2252412A1
CA2252412A1 CA002252412A CA2252412A CA2252412A1 CA 2252412 A1 CA2252412 A1 CA 2252412A1 CA 002252412 A CA002252412 A CA 002252412A CA 2252412 A CA2252412 A CA 2252412A CA 2252412 A1 CA2252412 A1 CA 2252412A1
Authority
CA
Canada
Prior art keywords
protein
green fluorescent
fluorescent protein
cells
gfp
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002252412A
Other languages
English (en)
Other versions
CA2252412C (fr
Inventor
William Gordon-Kamm
Dorothy A. Pierce
Benjamin Bowen
Dennis Bidney
Margit Ross
Christopher Scelonge
Mike Miller
Gary Sandahl
Lijuan Wang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pioneer Hi Bred International Inc
Original Assignee
Pioneer Hi-Bred International, Inc.
William Gordon-Kamm
Dorothy A. Pierce
Benjamin Bowen
Dennis Bidney
Margit Ross
Christopher Scelonge
Mike Miller
Gary Sandahl
Lijuan Wang
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pioneer Hi-Bred International, Inc., William Gordon-Kamm, Dorothy A. Pierce, Benjamin Bowen, Dennis Bidney, Margit Ross, Christopher Scelonge, Mike Miller, Gary Sandahl, Lijuan Wang filed Critical Pioneer Hi-Bred International, Inc.
Priority to CA2502460A priority Critical patent/CA2502460C/fr
Priority to CA2502657A priority patent/CA2502657C/fr
Publication of CA2252412A1 publication Critical patent/CA2252412A1/fr
Application granted granted Critical
Publication of CA2252412C publication Critical patent/CA2252412C/fr
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43595—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from coelenteratae, e.g. medusae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C12N15/821—Non-antibiotic resistance markers, e.g. morphogenetic, metabolic markers
    • C12N15/8212—Colour markers, e.g. beta-glucoronidase [GUS], green fluorescent protein [GFP], carotenoid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
CA002252412A 1996-05-01 1997-05-01 Utilisation de la proteine fluorescente verte comme marqueur de criblage pour la transformation de plantes Expired - Fee Related CA2252412C (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CA2502460A CA2502460C (fr) 1996-05-01 1997-05-01 Utilisation de la proteine verte fluorescente comme marqueur tamisable pour la transformation de plantes
CA2502657A CA2502657C (fr) 1996-05-01 1997-05-01 Utilisation de la proteine fluorescente verte comme marqueur de criblage pour la transformation de plantes

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US1634596P 1996-05-01 1996-05-01
US60/016,345 1996-05-01
PCT/US1997/007688 WO1997041228A2 (fr) 1996-05-01 1997-05-01 Utilisation de la proteine fluorescente verte comme marqueur de criblage pour la transformation de plantes

Related Child Applications (2)

Application Number Title Priority Date Filing Date
CA2502460A Division CA2502460C (fr) 1996-05-01 1997-05-01 Utilisation de la proteine verte fluorescente comme marqueur tamisable pour la transformation de plantes
CA2502657A Division CA2502657C (fr) 1996-05-01 1997-05-01 Utilisation de la proteine fluorescente verte comme marqueur de criblage pour la transformation de plantes

Publications (2)

Publication Number Publication Date
CA2252412A1 true CA2252412A1 (fr) 1997-11-06
CA2252412C CA2252412C (fr) 2005-10-25

Family

ID=21776667

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002252412A Expired - Fee Related CA2252412C (fr) 1996-05-01 1997-05-01 Utilisation de la proteine fluorescente verte comme marqueur de criblage pour la transformation de plantes

Country Status (8)

Country Link
US (1) US6486382B1 (fr)
EP (1) EP0904371B1 (fr)
AR (1) AR006928A1 (fr)
AT (1) ATE277177T1 (fr)
AU (1) AU730927B2 (fr)
CA (1) CA2252412C (fr)
DE (1) DE69730830T2 (fr)
WO (1) WO1997041228A2 (fr)

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AU2998397A (en) 1997-11-19
ATE277177T1 (de) 2004-10-15
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AU730927B2 (en) 2001-03-22
AR006928A1 (es) 1999-09-29
DE69730830T2 (de) 2005-09-22
US6486382B1 (en) 2002-11-26
DE69730830D1 (de) 2004-10-28
EP0904371B1 (fr) 2004-09-22
WO1997041228A2 (fr) 1997-11-06

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