BRPI9904104B1 - processo para produção de sacarídeos empregando uma enzima de formação de sacarídeo não redutor e uma enzima de liberação de trealose - Google Patents
processo para produção de sacarídeos empregando uma enzima de formação de sacarídeo não redutor e uma enzima de liberação de trealose Download PDFInfo
- Publication number
- BRPI9904104B1 BRPI9904104B1 BRPI9904104A BR9904104A BRPI9904104B1 BR PI9904104 B1 BRPI9904104 B1 BR PI9904104B1 BR PI9904104 A BRPI9904104 A BR PI9904104A BR 9904104 A BR9904104 A BR 9904104A BR PI9904104 B1 BRPI9904104 B1 BR PI9904104B1
- Authority
- BR
- Brazil
- Prior art keywords
- enzyme
- trehalose
- dna
- reducing
- reducing saccharide
- Prior art date
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- 238000004062 sedimentation Methods 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- 229910000029 sodium carbonate Inorganic materials 0.000 description 1
- 239000001488 sodium phosphate Substances 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 230000000087 stabilizing effect Effects 0.000 description 1
- WPLOVIFNBMNBPD-ATHMIXSHSA-N subtilin Chemical compound CC1SCC(NC2=O)C(=O)NC(CC(N)=O)C(=O)NC(C(=O)NC(CCCCN)C(=O)NC(C(C)CC)C(=O)NC(=C)C(=O)NC(CCCCN)C(O)=O)CSC(C)C2NC(=O)C(CC(C)C)NC(=O)C1NC(=O)C(CCC(N)=O)NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C1NC(=O)C(=C/C)/NC(=O)C(CCC(N)=O)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)CNC(=O)C(NC(=O)C(NC(=O)C2NC(=O)CNC(=O)C3CCCN3C(=O)C(NC(=O)C3NC(=O)C(CC(C)C)NC(=O)C(=C)NC(=O)C(CCC(O)=O)NC(=O)C(NC(=O)C(CCCCN)NC(=O)C(N)CC=4C5=CC=CC=C5NC=4)CSC3)C(C)SC2)C(C)C)C(C)SC1)CC1=CC=CC=C1 WPLOVIFNBMNBPD-ATHMIXSHSA-N 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/90—Isomerases (5.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01001—Alpha-amylase (3.2.1.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01002—Beta-amylase (3.2.1.2)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01003—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/0102—Alpha-glucosidase (3.2.1.20)
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP25839498 | 1998-09-11 | ||
| JP35225298 | 1998-12-11 | ||
| JP01693199A JP3958884B2 (ja) | 1998-09-11 | 1999-01-26 | 非還元性糖質生成酵素及びトレハロース遊離酵素ならびに該酵素を用いる糖質の製造方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| BR9904104A BR9904104A (pt) | 2000-10-17 |
| BRPI9904104B1 true BRPI9904104B1 (pt) | 2016-04-26 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BRPI9904104A BRPI9904104B1 (pt) | 1998-09-11 | 1999-09-13 | processo para produção de sacarídeos empregando uma enzima de formação de sacarídeo não redutor e uma enzima de liberação de trealose |
Country Status (10)
| Country | Link |
|---|---|
| US (3) | US7186535B1 (enExample) |
| EP (1) | EP0990704B1 (enExample) |
| JP (1) | JP3958884B2 (enExample) |
| KR (1) | KR100729498B1 (enExample) |
| CN (2) | CN1271203C (enExample) |
| AT (1) | ATE417110T1 (enExample) |
| BR (1) | BRPI9904104B1 (enExample) |
| DE (1) | DE69940045D1 (enExample) |
| DK (1) | DK0990704T3 (enExample) |
| ES (1) | ES2318884T3 (enExample) |
Families Citing this family (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7709230B2 (en) | 2001-04-27 | 2010-05-04 | Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo | Process for producing isomaltose and uses thereof |
| CN100415894C (zh) * | 2001-04-27 | 2008-09-03 | 株式会社林原生物化学研究所 | 异麦芽糖的制备方法及用途 |
| EP1582105A4 (en) | 2002-12-19 | 2006-02-01 | Hayashibara Biochem Lab | METHOD FOR PREVENTING THE WATER CONTENT CHANGES OF A COMPOSITION AND ITS USE |
| US7854922B2 (en) | 2003-02-13 | 2010-12-21 | Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo | Skin preparation for external use characterized by containing sugar derivative of α, α-trehalose |
| EP1600496B8 (en) * | 2003-02-13 | 2013-10-09 | Hayashibara Co., Ltd. | Method of inhibiting formation of volatile aldehydes and/or decomposition of fatty acids and use thereof |
| JP2005013227A (ja) * | 2003-06-06 | 2005-01-20 | Hayashibara Biochem Lab Inc | 加工品の品質保持方法 |
| US20070099869A1 (en) * | 2003-06-10 | 2007-05-03 | Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo | Association product of α-glycosyl α,α-trehalose with ionic metal compound |
| CN101232891A (zh) | 2005-08-11 | 2008-07-30 | 株式会社林原生物化学研究所 | 胶原产生增强剂及其用途 |
| CN101126101A (zh) * | 2006-08-18 | 2008-02-20 | Bapp酶工程有限公司 | 酒精酿造方法 |
| ES2436517T3 (es) | 2010-11-02 | 2014-01-02 | Abbott Laboratories | Fortificante de leche humana líquido concentrado estable |
| PT2759600T (pt) * | 2011-09-21 | 2018-07-06 | Hayashibara Co | Processo para produzir um pó contendo alfa,alfa-trealose cristalina di-hidratada |
| JP5993674B2 (ja) * | 2012-09-12 | 2016-09-14 | 株式会社林原 | α，α−トレハロース二含水結晶含有粉末の製造方法 |
| CN103194434B (zh) * | 2013-04-15 | 2014-08-13 | 山东天力药业有限公司 | 麦芽寡糖基海藻糖水解酶、酶基因、含该基因的重组表达载体及重组工程菌及酶的制备 |
| CN103194432B (zh) * | 2013-04-15 | 2014-08-20 | 山东天力药业有限公司 | 新型麦芽寡糖基海藻糖合成酶、酶基因、含该基因的重组表达载体及重组工程菌及酶的制备 |
| CN107058201A (zh) * | 2017-04-24 | 2017-08-18 | 江南大学 | 一种提高MTSase和MTHase产量的方法 |
| WO2021039833A1 (ja) | 2019-08-29 | 2021-03-04 | 国立大学法人愛媛大学 | 培養組織の製造方法、及び外用剤 |
Family Cites Families (43)
| Publication number | Priority date | Publication date | Assignee | Title |
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| JPS5823799A (ja) | 1981-08-03 | 1983-02-12 | 株式会社林原生物化学研究所 | 高純度マルト−スの製造方法 |
| JPS5872598A (ja) | 1981-10-26 | 1983-04-30 | Hayashibara Biochem Lab Inc | 高純度イソマルト−スの製造方法 |
| DK594084A (da) | 1984-12-12 | 1986-06-13 | Novo Industri As | Fremgangsmaade til stabilisering af extra-chromosomale elementer i bakterier under dyrkning |
| DK160947C (da) | 1986-04-17 | 1991-10-21 | Novo Industri As | Gen-udtrykkelsessystem |
| DK639689D0 (da) | 1989-12-18 | 1989-12-18 | Novo Nordisk As | Indfoering af dna i celler |
| DE69332781T2 (de) | 1992-12-28 | 2004-02-12 | Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo | Reinigung von Trehalose |
| JP3201526B2 (ja) | 1993-02-19 | 2001-08-20 | ノボ ノルディスク アクティーゼルスカブ | デンプン分解酵素 |
| JP3559585B2 (ja) | 1993-06-03 | 2004-09-02 | 株式会社林原生物化学研究所 | トレハロース遊離酵素とその製造方法並びに用途 |
| DK0713365T4 (da) | 1993-08-10 | 2003-02-17 | Csm Nederland Bv | Rugmel |
| AU695766B2 (en) | 1993-12-01 | 1998-08-20 | Novo Nordisk Biotech, Inc. | (Aspergillus) expression system |
| DE69418594T2 (de) | 1994-02-18 | 1999-09-16 | Tapematic S.P.A., Monza | Beschicktigungsanlage für Kassetten in einer automatischen Maschine für die Aufladung von Bändern in die Kassetten und damit durchgeführtes Verfahren zum Laden |
| ATE181107T1 (de) * | 1994-02-23 | 1999-06-15 | Hayashibara Biochem Lab | Nicht reduzierendes saccharid bildendes enzym, dieses kodierende dna, und deren herstellungen und verwendungen |
| JP3557272B2 (ja) | 1994-02-23 | 2004-08-25 | 株式会社林原生物化学研究所 | 組換え型酵素とその製造方法並びに用途 |
| KR100374449B1 (ko) * | 1994-03-07 | 2003-09-06 | 가부시끼가이샤 하야시바라 세이부쓰 가가꾸 겐꾸조 | 효소를인코우드하는디엔에이(dna),재조합디엔에이(dna)와효소,형질전환체및이들의제조방법과용도 |
| JP3559609B2 (ja) | 1994-03-07 | 2004-09-02 | 株式会社林原生物化学研究所 | 組換え型酵素とその製造方法並びに用途 |
| JP3557277B2 (ja) | 1994-06-25 | 2004-08-25 | 株式会社林原生物化学研究所 | 耐熱性トレハロース遊離酵素とその製造方法並びに用途 |
| JP3793590B2 (ja) | 1994-06-27 | 2006-07-05 | 株式会社林原生物化学研究所 | 非還元性糖質とその製造方法並びに用途 |
| JP3662972B2 (ja) | 1994-06-27 | 2005-06-22 | 株式会社林原生物化学研究所 | 非還元性糖質とその製造方法並びに用途 |
| WO1995034642A1 (en) | 1994-06-15 | 1995-12-21 | Kirin Beer Kabushiki Kaisha | Novel transferase and amylase, process for producing the enzymes,use thereof, and gene coding for the same |
| JP3557287B2 (ja) | 1994-06-24 | 2004-08-25 | 株式会社林原生物化学研究所 | 耐熱性非還元性糖質生成酵素とその製造方法並びに用途 |
| AU695080B2 (en) | 1994-06-24 | 1998-08-06 | Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo | Thermostable non-reducing saccharide-forming enzyme, its production and uses |
| JP4070250B2 (ja) | 1994-06-27 | 2008-04-02 | 株式会社林原生物化学研究所 | 還元性を低減させた糖質とその製造方法並びに用途 |
| TW426737B (en) | 1994-06-27 | 2001-03-21 | Hayashibara Biochem Lab | Saccharide composition with reduced reducibility, and preparation and uses thereof |
| JP3616166B2 (ja) | 1994-07-19 | 2005-02-02 | 株式会社林原生物化学研究所 | トレハロースとその製造方法並びに用途 |
| DE69529026T2 (de) | 1994-07-19 | 2003-07-17 | Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo, Okayama | Trehalose, ihre Herstellung und ihre Verwendung |
| JP3557289B2 (ja) | 1994-07-21 | 2004-08-25 | 株式会社林原生物化学研究所 | 非還元性糖質からトレハロースを遊離する組換え型耐熱性酵素 |
| JP3557288B2 (ja) | 1994-07-21 | 2004-08-25 | 株式会社林原生物化学研究所 | 還元性澱粉糖から末端にトレハロース構造を有する非還元性糖質を生成する組換え型耐熱性酵素 |
| JP3084609B2 (ja) | 1995-04-12 | 2000-09-04 | 株式会社林原生物化学研究所 | トレハロース含有シラップ |
| JP3624038B2 (ja) | 1995-12-11 | 2005-02-23 | 株式会社林原生物化学研究所 | 糖衣固形物及びその製造方法 |
| JPH09252719A (ja) | 1996-03-21 | 1997-09-30 | Kyokuto Internatl Corp | カット野菜・カット果物の鮮度保持剤 |
| JP3549366B2 (ja) | 1996-06-20 | 2004-08-04 | 株式会社林原生物化学研究所 | 飲食物の塩から味及び／又は旨味増強方法 |
| US5916881A (en) | 1996-10-07 | 1999-06-29 | Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo | High trehalose content syrup |
| JP3806498B2 (ja) | 1996-10-07 | 2006-08-09 | 株式会社林原生物化学研究所 | トレハロース高含有シラップとその製造方法並びに用途 |
| JP4034846B2 (ja) | 1996-12-10 | 2008-01-16 | 株式会社林原生物化学研究所 | 結晶性粉末糖質とその製造方法並びに用途 |
| TW466116B (en) | 1997-03-04 | 2001-12-01 | Hayashibara Biochem Lab | Reduction inhibitory agent for active-oxygen eliminating activity, method for inhibiting the reduction of said activity, and composition containing said agent |
| JPH11263795A (ja) | 1997-03-04 | 1999-09-28 | Hayashibara Biochem Lab Inc | 活性酸素消去能低減抑制剤 |
| JP4034862B2 (ja) | 1997-06-02 | 2008-01-16 | 株式会社林原生物化学研究所 | スクロースの後味改善方法とその用途 |
| JPH1160590A (ja) | 1997-08-22 | 1999-03-02 | Yakult Honsha Co Ltd | オリゴフコース誘導体又はオリゴラムノース誘導体及びその製造法 |
| JPH1172763A (ja) | 1997-08-29 | 1999-03-16 | Ando Electric Co Ltd | 音響光学変調器 |
| JP3782874B2 (ja) | 1997-09-05 | 2006-06-07 | 株式会社林原生物化学研究所 | コーヒーホワイトナー |
| JP4098855B2 (ja) | 1997-09-16 | 2008-06-11 | 株式会社林原生物化学研究所 | 経口摂取用固状成形物とその製造方法 |
| JPH11158075A (ja) | 1997-11-25 | 1999-06-15 | Hayashibara Biochem Lab Inc | 膵機能調節剤 |
| JP3908868B2 (ja) | 1997-12-26 | 2007-04-25 | 株式会社林原生物化学研究所 | トリメチルアミン生成抑制方法とその用途 |
-
1999
- 1999-01-26 JP JP01693199A patent/JP3958884B2/ja not_active Expired - Lifetime
- 1999-09-10 CN CNB03160126XA patent/CN1271203C/zh not_active Expired - Lifetime
- 1999-09-10 CN CNB991238966A patent/CN1180081C/zh not_active Expired - Lifetime
- 1999-09-11 KR KR1019990038890A patent/KR100729498B1/ko not_active Expired - Fee Related
- 1999-09-13 DK DK99307220T patent/DK0990704T3/da active
- 1999-09-13 DE DE69940045T patent/DE69940045D1/de not_active Expired - Lifetime
- 1999-09-13 ES ES99307220T patent/ES2318884T3/es not_active Expired - Lifetime
- 1999-09-13 AT AT99307220T patent/ATE417110T1/de not_active IP Right Cessation
- 1999-09-13 BR BRPI9904104A patent/BRPI9904104B1/pt not_active IP Right Cessation
- 1999-09-13 EP EP99307220A patent/EP0990704B1/en not_active Expired - Lifetime
- 1999-11-08 US US09/435,770 patent/US7186535B1/en not_active Expired - Fee Related
-
2006
- 2006-11-29 US US11/564,476 patent/US7582463B2/en not_active Expired - Fee Related
- 2006-11-29 US US11/564,493 patent/US7575900B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| JP2000228980A (ja) | 2000-08-22 |
| BR9904104A (pt) | 2000-10-17 |
| CN1504567A (zh) | 2004-06-16 |
| US7575900B2 (en) | 2009-08-18 |
| ATE417110T1 (de) | 2008-12-15 |
| EP0990704A3 (en) | 2000-04-26 |
| US7186535B1 (en) | 2007-03-06 |
| KR20000023095A (ko) | 2000-04-25 |
| EP0990704A2 (en) | 2000-04-05 |
| KR100729498B1 (ko) | 2007-06-15 |
| US20070281346A1 (en) | 2007-12-06 |
| US7582463B2 (en) | 2009-09-01 |
| CN1252442A (zh) | 2000-05-10 |
| DK0990704T3 (da) | 2009-02-09 |
| US20070218529A1 (en) | 2007-09-20 |
| CN1271203C (zh) | 2006-08-23 |
| EP0990704B1 (en) | 2008-12-10 |
| ES2318884T3 (es) | 2009-05-01 |
| JP3958884B2 (ja) | 2007-08-15 |
| CN1180081C (zh) | 2004-12-15 |
| DE69940045D1 (de) | 2009-01-22 |
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