BRPI0716207A2 - Transformação de planta sem seleção - Google Patents
Transformação de planta sem seleçãoInfo
- Publication number
- BRPI0716207A2 BRPI0716207A2 BRPI0716207-3A2A BRPI0716207A BRPI0716207A2 BR PI0716207 A2 BRPI0716207 A2 BR PI0716207A2 BR PI0716207 A BRPI0716207 A BR PI0716207A BR PI0716207 A2 BRPI0716207 A2 BR PI0716207A2
- Authority
- BR
- Brazil
- Prior art keywords
- plants
- plant cells
- selection
- cells
- plant transformation
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
- C12N15/8205—Agrobacterium mediated transformation
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
- A01H6/46—Gramineae or Poaceae, e.g. ryegrass, rice, wheat or maize
- A01H6/4684—Zea mays [maize]
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
- C12N15/821—Non-antibiotic resistance markers, e.g. morphogenetic, metabolic markers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
- C12N15/821—Non-antibiotic resistance markers, e.g. morphogenetic, metabolic markers
- C12N15/8212—Colour markers, e.g. beta-glucoronidase [GUS], green fluorescent protein [GFP], carotenoid
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8262—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield involving plant development
- C12N15/8265—Transgene containment, e.g. gene dispersal
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/25—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving enzymes not classifiable in groups C12Q1/26 - C12Q1/66
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Developmental Biology & Embryology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Physiology (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Mycology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
- Saccharide Compounds (AREA)
- Compounds Of Unknown Constitution (AREA)
- Inorganic Compounds Of Heavy Metals (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US84151906P | 2006-08-31 | 2006-08-31 | |
PCT/US2007/077370 WO2008028121A1 (en) | 2006-08-31 | 2007-08-31 | Plant transformation without selection |
Publications (2)
Publication Number | Publication Date |
---|---|
BRPI0716207A2 true BRPI0716207A2 (pt) | 2013-11-12 |
BRPI0716207B1 BRPI0716207B1 (pt) | 2018-09-18 |
Family
ID=38779916
Family Applications (4)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BRPI0716225-1A BRPI0716225B1 (pt) | 2006-08-31 | 2007-08-31 | Methods for the rapid production of a transgenic monocotyledonous plant for the expansion of a development priority window for the production of transgenic plants and for the screening of cells with respect to the transformation capacity |
BRPI0716207A BRPI0716207B1 (pt) | 2006-08-31 | 2007-08-31 | método para a identificação de plantas de milho transgênicas |
BR122017012510-9A BR122017012510B1 (pt) | 2006-08-31 | 2007-08-31 | Rocessos de produção de uma cultura de células de milho transformáveis e embriogênicas |
BRPI0716373-8A BRPI0716373B1 (pt) | 2006-08-31 | 2007-08-31 | "processes for production of a transgenic corn plant". |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BRPI0716225-1A BRPI0716225B1 (pt) | 2006-08-31 | 2007-08-31 | Methods for the rapid production of a transgenic monocotyledonous plant for the expansion of a development priority window for the production of transgenic plants and for the screening of cells with respect to the transformation capacity |
Family Applications After (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR122017012510-9A BR122017012510B1 (pt) | 2006-08-31 | 2007-08-31 | Rocessos de produção de uma cultura de células de milho transformáveis e embriogênicas |
BRPI0716373-8A BRPI0716373B1 (pt) | 2006-08-31 | 2007-08-31 | "processes for production of a transgenic corn plant". |
Country Status (12)
Country | Link |
---|---|
US (16) | US8124411B2 (pt) |
EP (5) | EP2390337B1 (pt) |
JP (3) | JP5350246B2 (pt) |
CN (5) | CN101528932B (pt) |
AT (1) | ATE497008T1 (pt) |
AU (3) | AU2007289111B2 (pt) |
BR (4) | BRPI0716225B1 (pt) |
CA (8) | CA2661181C (pt) |
DE (1) | DE602007012269D1 (pt) |
MX (3) | MX2009002250A (pt) |
WO (3) | WO2008028119A2 (pt) |
ZA (3) | ZA200901140B (pt) |
Families Citing this family (31)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20040210958A1 (en) * | 2003-03-19 | 2004-10-21 | Monsanto Technology Llc | A Novel Culture Method for Corn Transformation |
US7682829B2 (en) * | 2003-05-30 | 2010-03-23 | Monsanto Technology Llc | Methods for corn transformation |
JP4525314B2 (ja) * | 2004-11-26 | 2010-08-18 | 沖電気工業株式会社 | 光符号分割多重送受信方法及び光符号分割多重送受信装置 |
CA2661181C (en) * | 2006-08-31 | 2015-07-21 | Monsanto Technology Llc | Identifying transgenic corn plants without screening or selection |
CN101808505B (zh) | 2007-08-31 | 2013-05-15 | 孟山都技术公司 | 充分分离植物组织的方法和装置 |
JP2011120478A (ja) * | 2008-03-31 | 2011-06-23 | Japan Tobacco Inc | アグロバクテリウム菌による形質転換植物の作成方法 |
CN102639703A (zh) * | 2009-06-25 | 2012-08-15 | 先正达参股股份有限公司 | 土壤杆菌属(agrobacterium)介导甘蔗转化的方法 |
WO2011030083A1 (en) * | 2009-09-11 | 2011-03-17 | Imperial Innovations Limited | Method |
BR112012032535B1 (pt) | 2010-06-24 | 2019-11-19 | Syngenta Participations Ag | métodos para a transformação da cana-de-açúcar por intermédio de agrobacterium |
WO2012083137A1 (en) | 2010-12-17 | 2012-06-21 | Monsanto Technology Llc | Methods for improving competency of plant cells |
US20130091606A1 (en) * | 2011-06-24 | 2013-04-11 | Bayer Cropscience Lp | Methods and compositions for enhanced Agrobacterium-mediated transformation Efficiency |
US11807846B2 (en) * | 2012-04-29 | 2023-11-07 | Monsanto Technology Llc | Methods for transforming corn explants |
US11713465B2 (en) | 2013-11-21 | 2023-08-01 | Monsanto Technology, Llc | Methods for transforming wheat explants and compositions therefor |
DE102013020605A1 (de) | 2013-12-15 | 2015-06-18 | Kws Saat Ag | Selektionsmarker-freies rhizobiaceae-vermitteltes verfahren zur herstellung einer transgenen pflanze der gattung triticum |
CN103766221B (zh) * | 2014-02-10 | 2016-08-17 | 中国农业大学 | 一种单子叶植物的组织培养方法 |
EP3113601B1 (en) | 2014-03-07 | 2017-11-22 | Pioneer Hi-Bred International, Inc. | Methods and systems for extracting monocot embryos |
GB2533129A (en) * | 2014-12-11 | 2016-06-15 | Alkion Biopharma Sas | Bioreactor |
WO2016105185A1 (en) * | 2014-12-22 | 2016-06-30 | Keygene N.V. | Plant callus populations |
CA2989912A1 (en) * | 2015-06-29 | 2017-01-05 | Dow Agrosciences Llc | System for automated explant preparation and method of use |
JP6716876B2 (ja) * | 2015-09-14 | 2020-07-01 | 住友ゴム工業株式会社 | 遺伝的に改変された植物体を作製するための形質転換植物体の製造方法、及びこの製造方法により作製された、遺伝的に改変された形質転換植物体 |
US11330776B2 (en) * | 2015-10-30 | 2022-05-17 | Pioneer Hi-Bred International, Inc. | Methods and compositions for rapid plant transformation |
CN105660422B (zh) * | 2016-04-20 | 2017-09-29 | 遵义医学院 | 一种白及种苗的组织培养方法 |
EP3400962A1 (en) | 2017-05-09 | 2018-11-14 | Fraunhofer Gesellschaft zur Förderung der angewandten Forschung e.V. | Inhibitors of il-38 for use in treating and/or preventing cancer in a subject |
ES2962273T3 (es) * | 2017-06-02 | 2024-03-18 | Fraunhofer Ges Forschung | Procedimiento para la transformación automatizada de un paquete de células vegetales |
CN110226517B (zh) * | 2019-06-26 | 2021-06-01 | 北京市农林科学院 | 一种洋葱离体再生的方法及其使用的培养基 |
CN110607323A (zh) * | 2019-09-24 | 2019-12-24 | 四川育良生物科技有限公司 | 一种农杆菌介导水稻遗传转化方法 |
AR127146A1 (es) * | 2021-09-27 | 2023-12-20 | Monsanto Technology Llc | Composiciones y métodos para la transformación de explantes de embriones extirpados de semillas de monocotiledóneas |
AR127145A1 (es) * | 2021-09-27 | 2023-12-20 | Monsanto Technology Llc | Composiciones y métodos para la transformación de explantes de embriones extirpados de semillas de monocotiledóneas |
WO2023049893A1 (en) * | 2021-09-27 | 2023-03-30 | Monsanto Technology Llc | Compositions and methods for transformation of monocot seed excised embryo explants |
WO2023118136A1 (en) * | 2021-12-22 | 2023-06-29 | BASF Agricultural Solutions Seed US LLC | Improved screening method for genome edited events |
WO2023150657A2 (en) * | 2022-02-03 | 2023-08-10 | Pioneer Hi-Bred International, Inc. | Monocot leaf explant preparation |
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