BR0113034A - Produção maior de ácido 2-ceto-l-gulÈnico - Google Patents

Produção maior de ácido 2-ceto-l-gulÈnico

Info

Publication number
BR0113034A
BR0113034A BR0113034-0A BR0113034A BR0113034A BR 0113034 A BR0113034 A BR 0113034A BR 0113034 A BR0113034 A BR 0113034A BR 0113034 A BR0113034 A BR 0113034A
Authority
BR
Brazil
Prior art keywords
host cell
dkg
klg
keto
higher production
Prior art date
Application number
BR0113034-0A
Other languages
English (en)
Other versions
BR0113034B1 (pt
Inventor
Manoj Kumar
Fernando Valle
Veronique A Dartois
James A Hoch
Original Assignee
Genencor Int
Microgenomics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genencor Int, Microgenomics Inc filed Critical Genencor Int
Publication of BR0113034A publication Critical patent/BR0113034A/pt
Publication of BR0113034B1 publication Critical patent/BR0113034B1/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/58—Aldonic, ketoaldonic or saccharic acids
    • C12P7/60—2-Ketogulonic acid
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/26—Klebsiella (G)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Laminated Bodies (AREA)
  • Transition And Organic Metals Composition Catalysts For Addition Polymerization (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Production Of Multi-Layered Print Wiring Board (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)

Abstract

"PRODUçãO MAIOR DE áCIDO 2-CETO-L-GULÈNICO". Um método para aumento da produção biossintética de 2-KLG de uma célula hospedeira é descrito. Tal método compreende seleção de uma célula hospedeira que tenha um curso sintético pelo menos parcialmente intracelular que utilize 2,5-DKG para produzir 2-KLG; aumento do transporte do dito 2,5-DKG para a dita célula hospedeira enquanto mantendo a integridade da célula hospedeira; cultura da célula hospedeira para produzir o dito 2,5-DKG; e produção de 2-KLG. O transporte do 2,5-DKG é aumentando através da transformação do DNA da célula hospedeira codificando uma ou mais enzimas de transporte de 2,5-DKG para a célula hospedeira.
BRPI0113034-0A 2000-08-04 2001-08-03 Método para aumento da produção biossintética de ácido 2-ceto-L-gulônico (2-KLG) de uma célula hospedeira BR0113034B1 (pt)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US63329400A 2000-08-04 2000-08-04
US09/633,294 2000-08-04
US67703200A 2000-09-29 2000-09-29
US09/677,032 2000-09-29
PCT/US2001/024327 WO2002012528A2 (en) 2000-08-04 2001-08-03 Enhanced 2-keto-l-gulonic acid production

Publications (2)

Publication Number Publication Date
BR0113034A true BR0113034A (pt) 2005-08-16
BR0113034B1 BR0113034B1 (pt) 2014-09-16

Family

ID=27091840

Family Applications (3)

Application Number Title Priority Date Filing Date
BRPI0113032-3A BR0113032A (pt) 2000-08-04 2001-08-03 acentuação de produção industrial aumentando transporte de substrato
BRPI0113034-0A BR0113034B1 (pt) 2000-08-04 2001-08-03 Método para aumento da produção biossintética de ácido 2-ceto-L-gulônico (2-KLG) de uma célula hospedeira
BRPI0113036-6A BR0113036A (pt) 2000-08-04 2001-08-03 2,5-dkg permeases

Family Applications Before (1)

Application Number Title Priority Date Filing Date
BRPI0113032-3A BR0113032A (pt) 2000-08-04 2001-08-03 acentuação de produção industrial aumentando transporte de substrato

Family Applications After (1)

Application Number Title Priority Date Filing Date
BRPI0113036-6A BR0113036A (pt) 2000-08-04 2001-08-03 2,5-dkg permeases

Country Status (12)

Country Link
US (1) US20040029234A1 (pt)
EP (3) EP1305422B1 (pt)
JP (3) JP4975937B2 (pt)
CN (3) CN1469927A (pt)
AT (2) ATE318907T1 (pt)
AU (3) AU2001281018A1 (pt)
BR (3) BR0113032A (pt)
CA (4) CA2417969A1 (pt)
DE (1) DE60117566T2 (pt)
DK (1) DK1305421T3 (pt)
MX (3) MXPA03001025A (pt)
WO (3) WO2002012468A2 (pt)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2417969A1 (en) * 2000-08-04 2002-02-14 Genecor International, Inc. Enhancement of industrial production by increasing substrate transport
US6720168B2 (en) 2000-08-04 2004-04-13 Genencor International, Inc. 2,5-DKG permeases
US7229811B2 (en) 2001-08-03 2007-06-12 Genencor International, Inc. 2,5-diketo-D-gluconic acid (2,5-DKG) permeases
MXPA05012413A (es) 2003-05-22 2006-02-13 Genencor Int Cepas bacterianas disenadas metabolicamente que tienen transportadores de gluconato endogenos no funcionales.
JP4742521B2 (ja) * 2003-06-05 2011-08-10 味の素株式会社 目的物質の製造法
US7335496B2 (en) 2003-06-05 2008-02-26 Ajinomoto Co., Inc. Method for producing target substance
WO2006084725A1 (en) * 2005-02-11 2006-08-17 Dsm Ip Assets B.V. Novel gene sts 16
JP4980651B2 (ja) * 2006-06-09 2012-07-18 Ｊｘ日鉱日石エネルギー株式会社 菌体温度を制御する能力を有する細菌
CN105283554A (zh) * 2013-06-05 2016-01-27 朗泽科技新西兰有限公司 表现出提高的通过发酵途径的通量的重组微生物
CN114045243B (zh) * 2021-12-03 2023-12-26 山东天力药业有限公司 一种缩短生黑葡萄糖酸杆菌发酵周期的方法

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4757012A (en) * 1983-06-28 1988-07-12 Genentech, Inc. Ascorbic acid intermediates and process enzymes
US5047340A (en) * 1985-06-05 1991-09-10 The University Of Kentucky Research Foundation LAC+ saccharomyces cerevisiae
US5032514A (en) * 1988-08-08 1991-07-16 Genentech, Inc. Metabolic pathway engineering to increase production of ascorbic acid intermediates
US5376544A (en) * 1992-09-08 1994-12-27 Rutgers The State University Of New Jersey Enzymes for the production of 2-keto-L-gulonic acid
KR100346293B1 (ko) * 1993-10-08 2002-11-30 론자 아게 부티로베타인/크로토노베타인-l-카르니틴대사유전자와l-카르니틴의미생물학적제조에사용되는그것의용도
US5795716A (en) * 1994-10-21 1998-08-18 Chee; Mark S. Computer-aided visualization and analysis system for sequence evaluation
DE19523279A1 (de) * 1995-06-27 1997-01-09 Forschungszentrum Juelich Gmbh Verfahren zur mikrobiellen Herstellung von Aminosäuren mittels rekombinanter Mikroorganismen mit erhöhter Sekretionsrate
FR2736652B1 (fr) * 1995-07-13 1997-08-14 Univ Bourgogne Levures et bacteries transformees pour operer la fermentation malolactique dans les vins
US5958672A (en) * 1995-07-18 1999-09-28 Diversa Corporation Protein activity screening of clones having DNA from uncultivated microorganisms
US5795761A (en) * 1996-01-11 1998-08-18 Rutgers, The State University Of New Jersey Mutants of 2,5-diketo-D-gluconic acid (2,5-DKG) reductase A
US5783431A (en) * 1996-04-24 1998-07-21 Chromaxome Corporation Methods for generating and screening novel metabolic pathways
US5817490A (en) * 1996-05-17 1998-10-06 Eastman Chemical Company Enzymatic process for the manufacture of ascorbic acid 2-keto-L-gulonic acid and esters of 2-keto-L-gulonic acid
CN1180747A (zh) * 1996-10-18 1998-05-06 中国科学院上海生物工程研究中心 从葡萄糖发酵制备2－酮基－l－古龙酸的新方法
US5834231A (en) * 1996-10-24 1998-11-10 Archer Daniels Midland Co. Bacterial strains and use thereof in fermentation process for 2-keto-L-gulonic acid production
DE19644566A1 (de) * 1996-10-26 1998-04-30 Forschungszentrum Juelich Gmbh Mikrobielle Herstellung von Substanzen aus dem aromatischen Stoffwechsel / I
EP0839909A1 (en) * 1996-10-29 1998-05-06 Rijksuniversiteit te Groningen Nucleic acid sequences encoding citrate transporter proteins
CN1119414C (zh) * 1997-12-30 2003-08-27 中国科学院上海生物工程研究中心 突变的2,5二酮基－d－葡萄糖酸还原酶及其基因工程表达
US6368793B1 (en) * 1998-10-14 2002-04-09 Microgenomics, Inc. Metabolic selection methods
US6720168B2 (en) * 2000-08-04 2004-04-13 Genencor International, Inc. 2,5-DKG permeases
CA2417969A1 (en) * 2000-08-04 2002-02-14 Genecor International, Inc. Enhancement of industrial production by increasing substrate transport

Also Published As

Publication number Publication date
WO2002012468A3 (en) 2002-12-05
WO2002012481A2 (en) 2002-02-14
JP2004519214A (ja) 2004-07-02
WO2002012468A2 (en) 2002-02-14
JP4975938B2 (ja) 2012-07-11
CN1527880A (zh) 2004-09-08
ATE510017T1 (de) 2011-06-15
MXPA03001025A (es) 2003-11-25
AU2001283132A1 (en) 2002-02-18
EP1305423A2 (en) 2003-05-02
WO2002012528A2 (en) 2002-02-14
WO2002012528A3 (en) 2003-02-06
ATE318907T1 (de) 2006-03-15
EP1305422B1 (en) 2011-05-18
AU2001281068A1 (en) 2002-02-18
WO2002012481A3 (en) 2002-10-10
CN1458976A (zh) 2003-11-26
JP4975937B2 (ja) 2012-07-11
BR0113032A (pt) 2006-02-21
US20040029234A1 (en) 2004-02-12
CN1469927A (zh) 2004-01-21
CN100547073C (zh) 2009-10-07
DK1305421T3 (da) 2006-07-10
MXPA03001026A (es) 2003-09-22
EP1305421B1 (en) 2006-03-01
CA2417871A1 (en) 2002-02-14
DE60117566D1 (de) 2006-04-27
CA2820132A1 (en) 2002-02-14
EP1305422A2 (en) 2003-05-02
JP2004519213A (ja) 2004-07-02
MXPA03001030A (es) 2003-11-25
AU2001281018A1 (en) 2002-02-18
JP2004514421A (ja) 2004-05-20
EP1305421A2 (en) 2003-05-02
BR0113036A (pt) 2006-02-21
BR0113034B1 (pt) 2014-09-16
CA2417970A1 (en) 2002-02-14
CA2417970C (en) 2013-09-24
CA2417871C (en) 2011-11-15
CN100378222C (zh) 2008-04-02
DE60117566T2 (de) 2007-01-11
CA2417969A1 (en) 2002-02-14

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Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 10 (DEZ) ANOS CONTADOS A PARTIR DE 16/09/2014, OBSERVADAS AS CONDICOES LEGAIS.

B25D Requested change of name of applicant approved
B21F Lapse acc. art. 78, item iv - on non-payment of the annual fees in time

Free format text: REFERENTE A 15A ANUIDADE.

B24J Lapse because of non-payment of annual fees (definitively: art 78 iv lpi, resolution 113/2013 art. 12)

Free format text: EM VIRTUDE DA EXTINCAO PUBLICADA NA RPI 2385 DE 20-09-2016 E CONSIDERANDO AUSENCIA DE MANIFESTACAO DENTRO DOS PRAZOS LEGAIS, INFORMO QUE CABE SER MANTIDA A EXTINCAO DA PATENTE E SEUS CERTIFICADOS, CONFORME O DISPOSTO NO ARTIGO 12, DA RESOLUCAO 113/2013.