AU725516B2 - Biological systems for manufacture of polyhydroxyalkanoate polymers containing 4-hydroxyacids - Google Patents
Biological systems for manufacture of polyhydroxyalkanoate polymers containing 4-hydroxyacids Download PDFInfo
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- AU725516B2 AU725516B2 AU94968/98A AU9496898A AU725516B2 AU 725516 B2 AU725516 B2 AU 725516B2 AU 94968/98 A AU94968/98 A AU 94968/98A AU 9496898 A AU9496898 A AU 9496898A AU 725516 B2 AU725516 B2 AU 725516B2
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08G—MACROMOLECULAR COMPOUNDS OBTAINED OTHERWISE THAN BY REACTIONS ONLY INVOLVING UNSATURATED CARBON-TO-CARBON BONDS
- C08G63/00—Macromolecular compounds obtained by reactions forming a carboxylic ester link in the main chain of the macromolecule
- C08G63/02—Polyesters derived from hydroxycarboxylic acids or from polycarboxylic acids and polyhydroxy compounds
- C08G63/06—Polyesters derived from hydroxycarboxylic acids or from polycarboxylic acids and polyhydroxy compounds derived from hydroxycarboxylic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1025—Acyltransferases (2.3)
- C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1096—Transferases (2.) transferring nitrogenous groups (2.6)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/62—Carboxylic acid esters
- C12P7/625—Polyesters of hydroxy carboxylic acids
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Nutrition Science (AREA)
- General Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Cell Biology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Polyesters Or Polycarbonates (AREA)
- Biological Depolymerization Polymers (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US5937397P | 1997-09-19 | 1997-09-19 | |
| US60/059373 | 1997-09-19 | ||
| PCT/US1998/019659 WO1999014313A2 (en) | 1997-09-19 | 1998-09-18 | Biological systems for manufacture of polyhydroxylalkanoate polymers containing 4-hydroxyacids |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| AU9496898A AU9496898A (en) | 1999-04-05 |
| AU725516B2 true AU725516B2 (en) | 2000-10-12 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU94968/98A Ceased AU725516B2 (en) | 1997-09-19 | 1998-09-18 | Biological systems for manufacture of polyhydroxyalkanoate polymers containing 4-hydroxyacids |
Country Status (8)
| Country | Link |
|---|---|
| US (6) | US6316262B1 (enExample) |
| EP (1) | EP1015565B1 (enExample) |
| JP (4) | JP2001516574A (enExample) |
| AT (1) | ATE323152T1 (enExample) |
| AU (1) | AU725516B2 (enExample) |
| CA (1) | CA2303070C (enExample) |
| DE (2) | DE69838768T2 (enExample) |
| WO (1) | WO1999014313A2 (enExample) |
Families Citing this family (159)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7455999B2 (en) * | 1998-01-22 | 2008-11-25 | Metabolix, Inc. | Transgenic systems for the manufacture of poly (3-hydroxy-butyrate-co-3-hydroxyhexanoate) |
| MXPA00011401A (es) | 1998-05-22 | 2003-04-22 | Metabolix Inc | Composiciones de biopolimero de polimero de polihidroxialcanoato. |
| DE69938105T2 (de) | 1998-08-04 | 2009-01-29 | Metabolix, Inc., Cambridge | Herstellung von polyhydroxyalkanoaten aus polyolen |
| EP1700908B1 (en) * | 1998-08-18 | 2016-01-06 | Metabolix, Inc. | Transgenic microbial polyhydroxyalkanoate producers |
| DE69930276T2 (de) | 1998-08-18 | 2006-10-05 | Metabolix, Inc., Cambridge | Transgene polyhydroxyalkanoat produzierende mikroben |
| DK1163019T3 (da) | 1999-03-25 | 2008-03-03 | Metabolix Inc | Medicinske indretninger og anvendelser af polyhydroxyalkanoatpolymere |
| DE60019435T2 (de) | 1999-09-14 | 2006-02-23 | Tepha, Inc., Cambridge | Therapeutische anwendung von polymere die gamma-hydroxybutyrat enthalten |
| US7026526B2 (en) | 2000-02-11 | 2006-04-11 | Metabolix, Inc. | Multi-gene expression constructs containing modified inteins |
| US20020164729A1 (en) | 2000-07-21 | 2002-11-07 | Skraly Frank A. | Production of polyhydroxyalkanoates from polyols |
| US7230144B2 (en) | 2001-12-18 | 2007-06-12 | Metabolix Inc. | Methods of making intermediates from polyhydroxyalkanoates |
| US7867250B2 (en) | 2001-12-19 | 2011-01-11 | Nmt Medical, Inc. | Septal occluder and associated methods |
| US7318833B2 (en) | 2001-12-19 | 2008-01-15 | Nmt Medical, Inc. | PFO closure device with flexible thrombogenic joint and improved dislodgement resistance |
| CA2471871A1 (en) * | 2002-01-14 | 2003-07-24 | Nmt Medical, Inc. | Patent foramen ovale (pfo) closure method and device |
| US9241695B2 (en) | 2002-03-25 | 2016-01-26 | W.L. Gore & Associates, Inc. | Patent foramen ovale (PFO) closure clips |
| US7431729B2 (en) | 2002-06-05 | 2008-10-07 | Nmt Medical, Inc. | Patent foramen ovale (PFO) closure device with radial and circumferential support |
| AUPS318202A0 (en) * | 2002-06-26 | 2002-07-18 | Cochlear Limited | Parametric fitting of a cochlear implant |
| EP2365088B1 (en) | 2002-09-12 | 2015-05-27 | Metabolix, Inc. | Polyhydroxyalkanoate production by coenzyme a-dependent aldehyde dehydrogenase pathways |
| US7766820B2 (en) | 2002-10-25 | 2010-08-03 | Nmt Medical, Inc. | Expandable sheath tubing |
| US20040093017A1 (en) * | 2002-11-06 | 2004-05-13 | Nmt Medical, Inc. | Medical devices utilizing modified shape memory alloy |
| US9017373B2 (en) | 2002-12-09 | 2015-04-28 | W.L. Gore & Associates, Inc. | Septal closure devices |
| JP4353484B2 (ja) | 2003-01-22 | 2009-10-28 | 昭和電工株式会社 | アシルコエンザイムaを用いるアシル基転移酵素反応方法 |
| EP2860292B1 (en) | 2003-05-08 | 2020-07-22 | Tepha, Inc. | Polyhydroxyalkanoate medical textiles and fibers |
| US9861346B2 (en) | 2003-07-14 | 2018-01-09 | W. L. Gore & Associates, Inc. | Patent foramen ovale (PFO) closure device with linearly elongating petals |
| ES2428967T3 (es) | 2003-07-14 | 2013-11-12 | W.L. Gore & Associates, Inc. | Dispositivo tubular de cierre de foramen oval permeable (FOP) con sistema de retención |
| US8480706B2 (en) | 2003-07-14 | 2013-07-09 | W.L. Gore & Associates, Inc. | Tubular patent foramen ovale (PFO) closure device with catch system |
| EP1660167B1 (en) | 2003-08-19 | 2008-11-12 | NMT Medical, Inc. | Expandable sheath tubing |
| US7745197B1 (en) * | 2003-10-15 | 2010-06-29 | Newlight Technologies, Llc | Process for the utilization of ruminant animal methane emissions |
| US8735113B2 (en) | 2003-10-15 | 2014-05-27 | Newlight Technologies, Llc | Methods and systems for production of polyhydroxyalkanoate |
| US7579176B2 (en) * | 2003-10-15 | 2009-08-25 | Newlight Technologies, Llc | Method for the production of polyhydroxyalkanoic acid |
| US20050273119A1 (en) | 2003-12-09 | 2005-12-08 | Nmt Medical, Inc. | Double spiral patent foramen ovale closure clamp |
| US7871419B2 (en) | 2004-03-03 | 2011-01-18 | Nmt Medical, Inc. | Delivery/recovery system for septal occluder |
| US20050267524A1 (en) | 2004-04-09 | 2005-12-01 | Nmt Medical, Inc. | Split ends closure device |
| US8361110B2 (en) | 2004-04-26 | 2013-01-29 | W.L. Gore & Associates, Inc. | Heart-shaped PFO closure device |
| US7842053B2 (en) | 2004-05-06 | 2010-11-30 | Nmt Medical, Inc. | Double coil occluder |
| US8308760B2 (en) | 2004-05-06 | 2012-11-13 | W.L. Gore & Associates, Inc. | Delivery systems and methods for PFO closure device with two anchors |
| JP2007535997A (ja) | 2004-05-07 | 2007-12-13 | エヌエムティー メディカル, インコーポレイティッド | 管状中隔オクルーダーの捕捉機構 |
| JP4720114B2 (ja) * | 2004-05-20 | 2011-07-13 | 三菱化学株式会社 | オキザロ酢酸またはオキザロ酢酸誘導体の製造方法 |
| ATE473021T1 (de) | 2004-08-03 | 2010-07-15 | Tepha Inc | Nichtkräuselnde polyhydroxyalkanoatnähte |
| US8764848B2 (en) | 2004-09-24 | 2014-07-01 | W.L. Gore & Associates, Inc. | Occluder device double securement system for delivery/recovery of such occluder device |
| EP1863916B1 (en) * | 2005-03-16 | 2011-08-24 | Metabolix, Inc. | Chemically inducible expression of biosynthetic pathways |
| US8277480B2 (en) | 2005-03-18 | 2012-10-02 | W.L. Gore & Associates, Inc. | Catch member for PFO occluder |
| KR100979694B1 (ko) | 2005-05-24 | 2010-09-02 | 한국과학기술원 | 폴리락테이트 또는 그 공중합체 생성능을 가지는 세포 또는식물 및 이를 이용한 폴리락테이트 또는 그 공중합체의제조방법 |
| DK2781593T3 (da) * | 2005-08-22 | 2019-10-21 | Newlight Tech Inc | Fremgangsmåde til behandling af methanemissioner |
| JP2009512521A (ja) * | 2005-10-24 | 2009-03-26 | エヌエムティー メディカル, インコーポレイティッド | 放射線不透過性生体吸収性オクルダ |
| WO2007073566A1 (en) * | 2005-12-22 | 2007-06-28 | Nmt Medical, Inc. | Catch members for occluder devices |
| US8979921B2 (en) * | 2006-02-07 | 2015-03-17 | Tepha, Inc. | Polymeric, degradable drug-eluting stents and coatings |
| WO2007092417A1 (en) * | 2006-02-07 | 2007-08-16 | Tepha, Inc. | Toughened polylactic acid polymers and copolymers |
| ES2624589T3 (es) * | 2006-02-07 | 2017-07-17 | Tepha, Inc. | Métodos y dispositivos para la reparación del manguito de los rotadores |
| US9592325B2 (en) * | 2006-02-07 | 2017-03-14 | Tepha, Inc. | Polymeric, degradable drug-eluting stents and coatings |
| WO2007115125A2 (en) | 2006-03-31 | 2007-10-11 | Nmt Medical, Inc. | Deformable flap catch mechanism for occluder device |
| US8551135B2 (en) | 2006-03-31 | 2013-10-08 | W.L. Gore & Associates, Inc. | Screw catch mechanism for PFO occluder and method of use |
| US8870913B2 (en) | 2006-03-31 | 2014-10-28 | W.L. Gore & Associates, Inc. | Catch system with locking cap for patent foramen ovale (PFO) occluder |
| US9089627B2 (en) | 2006-07-11 | 2015-07-28 | Abbott Cardiovascular Systems Inc. | Stent fabricated from polymer composite toughened by a dispersed phase |
| WO2008010296A1 (en) * | 2006-07-21 | 2008-01-24 | Kaneka Corporation | Microorganism with replaced gene and process for producing polyester using the same |
| US20080082083A1 (en) * | 2006-09-28 | 2008-04-03 | Forde Sean T | Perforated expandable implant recovery sheath |
| WO2008062995A1 (en) * | 2006-11-21 | 2008-05-29 | Lg Chem, Ltd. | Copolymer comprising 4-hydroxybutyrate unit and lactate unit and its manufacturing method |
| US7943683B2 (en) | 2006-12-01 | 2011-05-17 | Tepha, Inc. | Medical devices containing oriented films of poly-4-hydroxybutyrate and copolymers |
| JP2010517638A (ja) * | 2007-02-02 | 2010-05-27 | トアニエ, インコーポレイテッド | 腱および靱帯を修復するシステムおよび方法 |
| CA2678946C (en) * | 2007-03-16 | 2019-02-12 | Genomatica, Inc. | Compositions and methods for the biosynthesis of 1,4-butanediol and its precursors |
| US9005242B2 (en) | 2007-04-05 | 2015-04-14 | W.L. Gore & Associates, Inc. | Septal closure device with centering mechanism |
| WO2008131167A1 (en) | 2007-04-18 | 2008-10-30 | Nmt Medical, Inc. | Flexible catheter system |
| US7947483B2 (en) | 2007-08-10 | 2011-05-24 | Genomatica, Inc. | Methods and organisms for the growth-coupled production of 1,4-butanediol |
| US8581040B2 (en) | 2007-08-30 | 2013-11-12 | Plant Sensory Systems, Llc | Methods of producing GABA |
| US8581041B2 (en) | 2007-08-30 | 2013-11-12 | Plant Sensory Systems, Llc | Methods of producing GABA |
| AU2008296450A1 (en) * | 2007-08-30 | 2009-03-12 | Plant Sensory System, Llc. | Alternative methods for the biosynthesis of GABA |
| TWI356705B (en) * | 2007-10-25 | 2012-01-21 | Internat Chlorella Co Ltd | Extracts from chlorella sorokiniana |
| SG186646A1 (en) * | 2007-12-13 | 2013-01-30 | Glycos Biotechnologies Inc | Microbial conversion of oils and fatty acids to high-value chemicals |
| US8287909B2 (en) * | 2007-12-19 | 2012-10-16 | Tepha, Inc. | Medical devices containing melt-blown non-wovens of poly-4-hydroxybutyrate and copolymers thereof |
| KR20090078925A (ko) * | 2008-01-16 | 2009-07-21 | 주식회사 엘지화학 | 폴리락테이트 또는 그 공중합체 생성능을 가지는 재조합미생물 및 이를 이용한 폴리락테이트 또는 그 공중합체의제조방법 |
| US20130165967A1 (en) | 2008-03-07 | 2013-06-27 | W.L. Gore & Associates, Inc. | Heart occlusion devices |
| US20090253154A1 (en) * | 2008-04-02 | 2009-10-08 | Immunosciences Lab., Inc. | Blood and saliva test for detection of delayed food allergy and intolerance against modified foods |
| US8487159B2 (en) * | 2008-04-28 | 2013-07-16 | Metabolix, Inc. | Production of polyhydroxybutyrate in switchgrass |
| EP2313491A4 (en) * | 2008-07-08 | 2011-12-07 | Opx Biotechnologies Inc | METHODS, COMPOSITIONS AND SYSTEMS FOR BIOSYNTHETIC BIOPRODUCTION OF 1,4-BUTANEDIOL |
| CA2735883C (en) | 2008-09-10 | 2020-05-05 | Genomatica, Inc. | Microorganisms for the production of 1,4-butanediol |
| EP3260532A1 (en) | 2008-12-12 | 2017-12-27 | CJ Research Center LLC | Green process and compositions for producing poly(5hv) and 5 carbon chemicals |
| JP5698001B2 (ja) * | 2009-02-10 | 2015-04-08 | 協和発酵バイオ株式会社 | アミノ酸の製造法 |
| CA2754108A1 (en) * | 2009-03-05 | 2010-09-10 | Metabolix, Inc. | Propagation of transgenic plants |
| SI3392340T1 (sl) | 2009-06-04 | 2022-05-31 | Genomatica, Inc. | Mikroorganizmi za proizvodnjo 1,4-BUTANDIOLA in pripadajoči postopki |
| LT2438036T (lt) | 2009-06-04 | 2017-06-26 | Genomatica, Inc. | Fermentacijos sultinio komponentų išskyrimo būdas |
| US20120029556A1 (en) | 2009-06-22 | 2012-02-02 | Masters Steven J | Sealing device and delivery system |
| US8956389B2 (en) | 2009-06-22 | 2015-02-17 | W. L. Gore & Associates, Inc. | Sealing device and delivery system |
| WO2011031566A1 (en) | 2009-08-27 | 2011-03-17 | Newlight Technologies, Llc | Process for the production of polyhydroxyalkanoates |
| US9181559B2 (en) | 2009-09-15 | 2015-11-10 | Metabolix, Inc. | Generation of high polyhydroxybutyrate producing oilseeds |
| AU2010306785B2 (en) * | 2009-10-13 | 2016-01-14 | Genomatica, Inc. | Microorganisms for the production of 1,4-butanediol, 4-hydroxybutanal, 4-hydroxybutyryl-CoA, putrescine and related compounds, and methods related thereto |
| WO2011063363A2 (en) * | 2009-11-20 | 2011-05-26 | Opx Biotechnologies, Inc. | Production of an organic acid and/or related chemicals |
| US8530210B2 (en) | 2009-11-25 | 2013-09-10 | Genomatica, Inc. | Microorganisms and methods for the coproduction 1,4-butanediol and gamma-butyrolactone |
| US20110166598A1 (en) * | 2009-12-02 | 2011-07-07 | Entrigue Surgical, Inc. | Devices and methods for tongue stabilization |
| RU2012132908A (ru) | 2010-02-11 | 2014-03-20 | Метаболикс, Инк. | Способ получения производных химических соединений |
| US8048661B2 (en) | 2010-02-23 | 2011-11-01 | Genomatica, Inc. | Microbial organisms comprising exogenous nucleic acids encoding reductive TCA pathway enzymes |
| US8637286B2 (en) | 2010-02-23 | 2014-01-28 | Genomatica, Inc. | Methods for increasing product yields |
| GB2478588A (en) * | 2010-03-12 | 2011-09-14 | G5 Internat Holdings Pte Ltd | Microbial production of polyhydroxyalkanoates (PHAs) using culture medium containing hydrogen |
| PT2558133T (pt) | 2010-03-26 | 2020-04-23 | Tepha Inc | Revestimentos para o fabrico e aplicação de dispositivos médicos de polihidroxialcanqato |
| ES2523928T3 (es) | 2010-06-15 | 2014-12-02 | Tepha, Inc. | Dispositivos médicos que contienen no-tejidos hilados en seco de poli-4-hidroxibutirato y copolímeros |
| US9511169B2 (en) | 2010-06-15 | 2016-12-06 | Tepha, Inc. | Medical devices containing dry spun non-wovens of poly-4-hydroxybutyrate and copolymers with anisotropic properties |
| EP2425865A1 (en) | 2010-08-06 | 2012-03-07 | Aesculap AG | Medicinal thread having a polyhydroxyalkanoate coating |
| WO2012037324A2 (en) | 2010-09-15 | 2012-03-22 | Metabolix, Inc. | Increasing carbon flow for polyhydroxybutyrate production in biomass crops |
| ES2644478T3 (es) | 2010-11-09 | 2017-11-29 | Tepha, Inc. | Implantes cocleares liberadores de fármacos |
| US8956835B2 (en) | 2010-11-24 | 2015-02-17 | Suny Research Foundation | Methods for producing polyhydroxyalkanoates from biodiesel-glycerol |
| US9078634B2 (en) | 2011-01-27 | 2015-07-14 | Cryosa, Llc | Apparatus and methods for treatment of obstructive sleep apnea utilizing cryolysis of adipose tissues |
| WO2012122343A2 (en) | 2011-03-08 | 2012-09-13 | Newlight Technologies, Llc | Polyhydroxyalkanoate production method |
| EP2702091B1 (en) | 2011-04-29 | 2019-02-13 | CJ CheilJedang Corporation | Process for latex production by melt emulsification |
| US9663791B2 (en) | 2011-04-29 | 2017-05-30 | Cj Research Center Llc | Green process for producing polyhydroxyalkanoates and chemicals using a renewable feedstock |
| WO2012170793A1 (en) | 2011-06-08 | 2012-12-13 | Metabolix, Inc. | Biorefinery process for thf production |
| MY165731A (en) | 2011-07-29 | 2018-04-20 | Mitsui Chemicals Inc | Microorganism having carbon dioxide fixation cycle introduced thereinto |
| US20140170714A1 (en) | 2011-08-10 | 2014-06-19 | Metabolix, Inc. | Post process purification for gamma-butyrolactone production |
| US9770232B2 (en) | 2011-08-12 | 2017-09-26 | W. L. Gore & Associates, Inc. | Heart occlusion devices |
| EP2754717A4 (en) * | 2011-09-05 | 2015-08-05 | Riken | PROCESS FOR PREPARING POLYHYDROXYALKANOATE WITH LONG CHAIN STRUCTURE |
| EP3266806A1 (en) | 2011-09-27 | 2018-01-10 | Tepha, Inc. | A method of making an article comrising poly-4-hydroxybutyrate or a copolymer thereof |
| JP6199872B2 (ja) | 2011-10-25 | 2017-09-20 | マローネ バイオ イノベーションズ,インコーポレイテッド | クロモバクテリウム(Chromobacterium)配合物、組成物、代謝産物、およびそれらの使用 |
| US8940509B2 (en) | 2011-11-02 | 2015-01-27 | Genomatica, Inc. | Microorganisms and methods for the production of caprolactone |
| US12060597B2 (en) | 2011-12-02 | 2024-08-13 | Newlight Technologies, Inc. | Polyhydroxyalkanoate production methods and systems for same |
| US20200347417A1 (en) | 2012-03-29 | 2020-11-05 | Newlight Technologies, Inc | Polyhydroxyalkanoate production methods and materials and microorganisms used in same |
| US9085784B1 (en) | 2012-03-29 | 2015-07-21 | Newlight Technologies, Llc | Polyhydroxyalkanoate production methods and materials and microorganisms used in same |
| EP2836601A1 (en) * | 2012-04-11 | 2015-02-18 | Helmholtz-Zentrum für Infektionsforschung GmbH | Pha-producing genetically engineered microorganisms |
| EP3238751B1 (en) | 2012-05-21 | 2020-07-08 | Tepha, Inc. | Resorbable bioceramic compositions of poly-4-hydroxybutyrate and copolymers |
| EP3831951A3 (en) | 2012-06-04 | 2021-09-15 | Genomatica, Inc. | Microorganisms and methods for production of 4-hydroxybutyrate, 1,4-butanediol and related compounds |
| BR112014030203B1 (pt) | 2012-06-08 | 2021-10-13 | Cj Cheiljedang Corporation | Processo para produção de produtos de ácido acrílico de base biológica |
| WO2014028943A1 (en) | 2012-08-17 | 2014-02-20 | Metabolix, Inc. | Biobased rubber modifiers for polymer blends |
| WO2014058655A1 (en) | 2012-10-10 | 2014-04-17 | Metabolix | Polyhydroxyalkanoate copolymer compositions and methods of making the same |
| US20160068463A1 (en) | 2012-11-14 | 2016-03-10 | Metabolix, Inc. | Production of salts of 4-hydroxybutyrate using biobased raw materials |
| US10149923B2 (en) | 2013-01-15 | 2018-12-11 | Tepha, Inc. | Implants for soft and hard tissue regeneration |
| US10828019B2 (en) | 2013-01-18 | 2020-11-10 | W.L. Gore & Associates, Inc. | Sealing device and delivery system |
| MY172023A (en) | 2013-01-24 | 2019-11-12 | Mitsui Chemicals Inc | Microorganism having carbon dioxide fixation cycle introduced thereinto |
| WO2014127053A2 (en) | 2013-02-13 | 2014-08-21 | Metabolix, Inc. | Process for ultra pure chemical production from biobased raw starting materials |
| US10201640B2 (en) | 2013-03-13 | 2019-02-12 | Tepha, Inc. | Ultrafine electrospun fibers of poly-4-hydroxybutyrate and copolymers thereof |
| WO2015026964A1 (en) | 2013-08-20 | 2015-02-26 | Tepha, Inc. | Closed cell foams including poly-4-hydroxybutyrate and copolymers thereof |
| US9687585B2 (en) | 2013-08-20 | 2017-06-27 | Tepha, Inc. | Thermoformed poly-4-hydroxybutyrate medical implants |
| US9302029B2 (en) | 2013-10-31 | 2016-04-05 | Tepha, Inc. | Pultrusion of poly-4-hydroxybutyrate and copolymers thereof |
| US9480780B2 (en) | 2013-11-05 | 2016-11-01 | Tepha, Inc. | Compositions and devices of poly-4-hydroxybutyrate |
| US10485535B2 (en) | 2013-12-19 | 2019-11-26 | Tornier, Inc. | High-strength bioabsorbable suture |
| EP3086820B1 (en) | 2013-12-26 | 2020-10-28 | Tepha, Inc. | Medical implants including laminates of poly-4-hydroxybutyrate and copolymers thereof |
| US9457127B2 (en) | 2014-03-18 | 2016-10-04 | Tepha, Inc. | Micro-fiber webs of poly-4-hydroxybutyrate and copolymers thereof produced by centrifugal spinning |
| EP3137015B1 (en) | 2014-04-30 | 2022-01-26 | Tepha, Inc. | Three-dimensional resorbable implants for tissue reinforcement and hernia repair |
| EP3142716B1 (en) | 2014-05-16 | 2018-04-25 | Tepha, Inc. | Medical devices containing dry spun non-wovens of poly-4-hydroxybutyrate and copolymers with anisotropic properties |
| US9808230B2 (en) | 2014-06-06 | 2017-11-07 | W. L. Gore & Associates, Inc. | Sealing device and delivery system |
| EP3180040B1 (en) | 2014-08-15 | 2020-05-13 | Tepha, Inc. | Self-retaining sutures of poly-4-hydroxybutyrate and copolymers thereof |
| EP3183012B1 (en) | 2014-08-20 | 2017-12-20 | Tepha, Inc. | Thermoformed poly-4-hydroxybutyrate medical implants |
| EP3197512B1 (en) | 2014-09-22 | 2020-05-06 | Tepha, Inc. | Oriented p4hb implants containing antimicrobial agents |
| EP3200736B8 (en) | 2014-10-01 | 2020-06-17 | CryOSA, Inc. | Apparatus for treatment of obstructive sleep apnea utilizing cryolysis of adipose tissues |
| US9555155B2 (en) | 2014-12-11 | 2017-01-31 | Tepha, Inc. | Methods of orienting multifilament yarn and monofilaments of poly-4-hydroxybutyrate and copolymers thereof |
| US10626521B2 (en) | 2014-12-11 | 2020-04-21 | Tepha, Inc. | Methods of manufacturing mesh sutures from poly-4-hydroxybutyrate and copolymers thereof |
| EP3365427B1 (en) | 2015-10-23 | 2020-08-19 | Metabolic Explorer | Microorganism modified for the assimilation of levulinic acid |
| US20190023632A1 (en) | 2016-01-06 | 2019-01-24 | Archer Daniels Midland Company | Process for producing 1,3-butanediol and for optionally further producing (r)-3-hydroxybutyl (r)-3-hydroxybutyrate |
| KR101774431B1 (ko) * | 2016-01-28 | 2017-09-05 | 한국과학기술원 | 자일로즈로부터 폴리(락테이트-co-글라이콜레이트) 또는 그 공중합체 생산능을 가지는 재조합 미생물 및 이를 이용한 폴리(락테이트-co-글라이콜레이트) 또는 그 공중합체의 제조방법 |
| WO2018217574A1 (en) | 2017-05-25 | 2018-11-29 | Tepha, Inc. | Continuous formation of tubes of poly-4-hydroxybutyrate and copolymers thereof |
| CN109112151B (zh) * | 2017-06-23 | 2021-10-29 | 北京蓝晶微生物科技有限公司 | 一种精细调控共聚物中4-羟基丁酸组成比例的基因盒及其应用 |
| WO2019050936A1 (en) | 2017-09-06 | 2019-03-14 | Tepha, Inc. | CALANDERATED SURGICAL TRELLIS COMPRISING POLYHYDROXYALKANOATES |
| ES2916298T3 (es) | 2017-12-04 | 2022-06-29 | Tepha Inc | Implantes médicos de poli-4-hidroxibutirato termoformado con membrana al vacío |
| CA3102277C (en) | 2018-06-11 | 2024-11-12 | Tepha Inc | PROCESSES FOR 3D PRINTING POLY-4-HYDROXYBUTYRATE AND COPOLYMERS |
| ES2956815T3 (es) | 2018-10-29 | 2023-12-28 | Tepha Inc | Procedimientos de fabricación de suturas de malla a partir de poli-4-hidroxibutirato y sus copolímeros |
| TWI827804B (zh) * | 2019-03-06 | 2024-01-01 | 日商三菱商事生命科學股份有限公司 | 蕈菌絲體之製造方法、含有蕈菌絲體的組成物、以及使用了蕈菌絲體之食品材料 |
| AU2020293986A1 (en) | 2019-06-10 | 2022-01-06 | Nuseed Nutritional Us Inc. | Transgenic land plants that express a polyhydroxyalkanoate synthase seed specifically with cytosolic localization |
| FR3109290B1 (fr) | 2020-04-17 | 2025-04-11 | Ph Tech | Dispositif médical comprenant une matrice biologique acellulaire et au moins un polymère |
| FR3110077B1 (fr) | 2020-05-12 | 2024-12-06 | Ph Tech | Procédé de fabrication d’un dispositif médical en trois dimensions et dispositif médical obtenu |
| FR3113371A1 (fr) | 2020-08-12 | 2022-02-18 | Ph Tech | Dispositif médical comprenant un assemblage d’éléments de matrices biologiques acellulaires et au moins un polymère |
| WO2022192168A1 (en) | 2021-03-11 | 2022-09-15 | Tepha, Inc. | Breast reconstruction implant |
| CN113832084B (zh) * | 2021-09-29 | 2024-01-05 | 北京化工大学 | 一种生产聚羟基脂肪酸酯的溶藻弧菌及其应用 |
| WO2023130069A2 (en) | 2021-12-30 | 2023-07-06 | Cryosa, Inc. | Systems and methods for treatment of obstructive sleep apnea |
| AU2023354862A1 (en) * | 2022-09-30 | 2025-05-01 | Kimberly-Clark Worldwide, Inc. | In vitro bioproduction of specific chain length poly(hydroxyalkanoate) monomers |
Family Cites Families (31)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS575693A (en) | 1980-06-13 | 1982-01-12 | Ajinomoto Co Inc | Production of l-arginine through fermentation process |
| IE50442B1 (en) * | 1980-11-21 | 1986-04-16 | Loctite Corp | Two part adhesive composition |
| US5250430A (en) | 1987-06-29 | 1993-10-05 | Massachusetts Institute Of Technology | Polyhydroxyalkanoate polymerase |
| US5245023A (en) | 1987-06-29 | 1993-09-14 | Massachusetts Institute Of Technology | Method for producing novel polyester biopolymers |
| US4876331A (en) | 1987-08-18 | 1989-10-24 | Mitsubishi Kasei Corporation | Copolyester and process for producing the same |
| US4876831A (en) * | 1988-03-14 | 1989-10-31 | Runyon John F | Folding modular building structure |
| US5004664A (en) | 1989-02-27 | 1991-04-02 | Xerox Corporation | Toner and developer compositions containing biodegradable semicrystalline polyesters |
| US5371002A (en) | 1989-06-07 | 1994-12-06 | James Madison University | Method of production of poly-beta-hydroxyalkanoate copolymers |
| ES2131489T3 (es) | 1989-07-10 | 2003-05-16 | Massachusetts Inst Technology | Una planta o una celula vegetal recombinante capaz de producir polihidroxibutirato u otro polihidroxialcanoato. |
| IE893328A1 (en) | 1989-10-16 | 1991-04-24 | Mcdaid Denis | Telephone lock |
| GB9108756D0 (en) | 1991-04-24 | 1991-06-12 | Ici Plc | Production of polyalkanoate in plants |
| US5286842A (en) | 1991-07-01 | 1994-02-15 | Mitsubishi Kasei Corporation | Process for producing a biodegradable polymer |
| GB9115245D0 (en) | 1991-07-16 | 1991-08-28 | Ici Plc | Production of polyalkanoate |
| CA2113412C (en) | 1991-07-19 | 2000-01-25 | Christopher R. Somerville | Transgenic plants producing polyhydroxyalkanoates |
| US5610041A (en) | 1991-07-19 | 1997-03-11 | Board Of Trustees Operating Michigan State University | Processes for producing polyhydroxybutyrate and related polyhydroxyalkanoates in the plastids of higher plants |
| PH30131A (en) | 1991-08-07 | 1997-01-21 | Ajinomoto Kk | Process for producing l-glutamic acid by fermentation |
| JP2777757B2 (ja) | 1991-09-17 | 1998-07-23 | 鐘淵化学工業株式会社 | 共重合体およびその製造方法 |
| GB9223332D0 (en) * | 1992-11-06 | 1992-12-23 | Ici Plc | Production of polyhydroxyalkanoate in plants |
| WO1994012014A1 (en) | 1992-11-20 | 1994-06-09 | Agracetus, Inc. | Transgenic cotton plants producing heterologous bioplastic |
| JP3263710B2 (ja) | 1992-12-11 | 2002-03-11 | 高砂香料工業株式会社 | 生分解性光学活性ポリマー及びその製造方法 |
| US6849427B1 (en) | 1993-03-12 | 2005-02-01 | Immulogic Pharmaceutical Corp. | Nucleic acids encoding a house dust mite allergen, Der p VII, and uses therefor |
| IL108947A0 (en) | 1993-03-12 | 1994-06-24 | Osteopharm Ltd | Bone stimulating factor |
| JP3241505B2 (ja) | 1993-08-11 | 2001-12-25 | 高砂香料工業株式会社 | 生分解性光学活性コポリマー及びその製造方法 |
| ID23491A (id) | 1994-01-28 | 1995-09-07 | Procter & Gamble | Kopolimer-kopolimer yang dapat dibiodegradasi dan baha-bahan plastik yang terdiri dari kopolimer-kopolimer yang dapat dibiodegradasi |
| BR9506664A (pt) | 1994-01-28 | 1997-09-16 | Procter & Gamble | Copolímeros biodegradâveis e artigos plásticos compreendendo copolímeros biodegradâveis de 3-hidroxihexanoato |
| CA2125313A1 (en) * | 1994-06-07 | 1995-12-08 | Gerald E. O'grady | Method and apparatus for producing electrical energy within the body of a ruminant |
| US5563239A (en) | 1994-11-09 | 1996-10-08 | Eastman Chemical Company | Composition and process for the production of poly(3-hydroxyalkanoates) |
| BE1008983A6 (fr) | 1994-12-30 | 1996-10-01 | Faco Sa | Bigoudi. |
| US5763270A (en) | 1995-06-07 | 1998-06-09 | Genemedicine, Inc. | Plasmid for delivery of nucleic acids to cells and methods of use |
| GB9602796D0 (en) * | 1996-02-12 | 1996-04-10 | Innes John Centre Innov Ltd | Genetic control of plant growth and development |
| US6117658A (en) * | 1997-02-13 | 2000-09-12 | James Madison University | Methods of making polyhydroxyalkanoates comprising 4-hydroxybutyrate monomer units |
-
1998
- 1998-09-18 AT AT98948384T patent/ATE323152T1/de not_active IP Right Cessation
- 1998-09-18 AU AU94968/98A patent/AU725516B2/en not_active Ceased
- 1998-09-18 US US09/156,809 patent/US6316262B1/en not_active Expired - Lifetime
- 1998-09-18 DE DE69838768T patent/DE69838768T2/de not_active Expired - Lifetime
- 1998-09-18 CA CA2303070A patent/CA2303070C/en not_active Expired - Fee Related
- 1998-09-18 WO PCT/US1998/019659 patent/WO1999014313A2/en not_active Ceased
- 1998-09-18 DE DE69834199T patent/DE69834199T2/de not_active Expired - Lifetime
- 1998-09-18 EP EP98948384A patent/EP1015565B1/en not_active Expired - Lifetime
- 1998-09-18 JP JP2000511853A patent/JP2001516574A/ja not_active Withdrawn
-
2001
- 2001-11-09 US US10/006,915 patent/US6689589B2/en not_active Expired - Lifetime
-
2003
- 2003-05-09 JP JP2003132325A patent/JP2003310262A/ja not_active Withdrawn
-
2004
- 2004-02-06 US US10/773,916 patent/US7081357B2/en not_active Expired - Fee Related
-
2005
- 2005-10-07 US US11/245,891 patent/US7229804B2/en not_active Expired - Fee Related
-
2007
- 2007-04-16 US US11/735,888 patent/US20100093043A1/en not_active Abandoned
- 2007-10-25 US US11/924,531 patent/US20110008856A1/en not_active Abandoned
-
2008
- 2008-12-24 JP JP2008328226A patent/JP2009082147A/ja active Pending
- 2008-12-24 JP JP2008328227A patent/JP2009171960A/ja active Pending
Non-Patent Citations (2)
| Title |
|---|
| FEMS MICROBIOLOGY LETTERS VOL 153 P411-418 (1997) * |
| J OF BIOTECHNOLOGY VOL 58 P33-38 (1997) * |
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| DE69838768T2 (de) | 2008-10-30 |
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| DE69834199T2 (de) | 2006-12-14 |
| DE69838768D1 (de) | 2008-01-03 |
| JP2001516574A (ja) | 2001-10-02 |
| WO1999014313A3 (en) | 1999-06-03 |
| EP1015565A2 (en) | 2000-07-05 |
| US6689589B2 (en) | 2004-02-10 |
| CA2303070C (en) | 2011-03-15 |
| US6316262B1 (en) | 2001-11-13 |
| WO1999014313A2 (en) | 1999-03-25 |
| EP1015565B1 (en) | 2006-04-12 |
| ATE323152T1 (de) | 2006-04-15 |
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