ATE520777T1 - Varianten der löslichen pyrroloquinolin quinon- abhängigen glukose dehydrogenase - Google Patents

Varianten der löslichen pyrroloquinolin quinon- abhängigen glukose dehydrogenase

Info

Publication number
ATE520777T1
ATE520777T1 AT01988775T AT01988775T ATE520777T1 AT E520777 T1 ATE520777 T1 AT E520777T1 AT 01988775 T AT01988775 T AT 01988775T AT 01988775 T AT01988775 T AT 01988775T AT E520777 T1 ATE520777 T1 AT E520777T1
Authority
AT
Austria
Prior art keywords
variants
gdh
pyrroloquinoline quinone
dependent glucose
glucose dehydrogenase
Prior art date
Application number
AT01988775T
Other languages
English (en)
Inventor
Peter Kratzsch
Rainer Schmuck
Daniela Bunk
Zhixin Shao
Detlef Thym
Wolfgang-Reinhold Knappe
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Application granted granted Critical
Publication of ATE520777T1 publication Critical patent/ATE520777T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Nitrogen Condensed Heterocyclic Rings (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
AT01988775T 2000-10-27 2001-10-20 Varianten der löslichen pyrroloquinolin quinon- abhängigen glukose dehydrogenase ATE520777T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP00123512 2000-10-27
EP00127294 2000-12-19
PCT/EP2001/012148 WO2002034919A1 (en) 2000-10-27 2001-10-20 Variants of soluble pyrroloquinoline quinone-dependent glucose dehydrogenase

Publications (1)

Publication Number Publication Date
ATE520777T1 true ATE520777T1 (de) 2011-09-15

Family

ID=26071553

Family Applications (1)

Application Number Title Priority Date Filing Date
AT01988775T ATE520777T1 (de) 2000-10-27 2001-10-20 Varianten der löslichen pyrroloquinolin quinon- abhängigen glukose dehydrogenase

Country Status (12)

Country Link
US (3) US20030104595A1 (de)
EP (1) EP1332216B1 (de)
JP (2) JP3845618B2 (de)
KR (1) KR100519902B1 (de)
CN (1) CN1288246C (de)
AT (1) ATE520777T1 (de)
AU (2) AU1596602A (de)
BR (1) BRPI0114962B8 (de)
CA (1) CA2427029C (de)
HK (1) HK1074217A1 (de)
PL (1) PL205561B1 (de)
WO (1) WO2002034919A1 (de)

Families Citing this family (45)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE435277T1 (de) * 2000-02-11 2009-07-15 Millennium Pharm Inc 18477, eine menschliche proteinkinase und deren verwendung
KR100519902B1 (ko) * 2000-10-27 2005-10-10 에프. 호프만-라 로슈 아게 가용성 피롤로퀴놀린 퀴논 의존성 글루코오스 디히드로게나아제의 변이체
JP2003093071A (ja) * 2001-09-26 2003-04-02 Koji Hayade グルコース脱水素酵素
US6855556B2 (en) * 2002-01-04 2005-02-15 Becton, Dickinson And Company Binding protein as biosensors
EP1367120A3 (de) * 2002-05-27 2004-06-02 Toyo Boseki Kabushiki Kaisha Modifizierte pyrroloquinolin quinon-abhängigen glukose Dehydrogenase mit höher Substratspezifität und Stabilität
JPWO2004005499A1 (ja) * 2002-07-04 2005-11-04 早出　広司 グルコース脱水素酵素
JP4528624B2 (ja) * 2002-09-27 2010-08-18 ディーエスエム アイピー アセッツ ビー．ブイ． アルデヒドデヒドロゲナーゼ遺伝子
DE60331477D1 (de) 2002-12-24 2010-04-08 Ikeda Food Res Co Ltd Coenzymbindende glukosedehydrogenase
JP4415282B2 (ja) * 2003-03-24 2010-02-17 東洋紡績株式会社 基質特異性または安定性に優れたピロロキノリンキノン（ｐｑｑ）依存性グルコースデヒドロゲナーゼ改変体
DE10320259A1 (de) 2003-05-07 2004-11-25 Bayer Technology Services Gmbh Neuartige Glukose Dehydrogenase und ihre Herstellung
JP2005328793A (ja) * 2004-05-21 2005-12-02 Toyobo Co Ltd 基質特異性に優れたピロロキノリンキノン（ｐｑｑ）依存性グルコースデヒドロゲナーゼ改変体
WO2005026340A1 (ja) * 2003-09-08 2005-03-24 Toyo Boseki Kabushiki Kaisha 基質特異性に優れたピロロキノリンキノン（ｐｑｑ）依存性グルコースデヒドロゲナーゼ改変体
EP1600503A1 (de) * 2004-05-14 2005-11-30 Amano Enzyme Inc. Modifizierte pyrroloquinolin quinon-abhängige Glukose Dehydrogenase
US7037698B2 (en) 2004-05-19 2006-05-02 Amano Enzyme Inc. Pyrroloquinoline quinone-dependent glucose dehydrogenase
US20060003400A1 (en) * 2004-06-30 2006-01-05 Byrd Patricia A Methods and compositions for characterizing a redox reagent system enzyme
BRPI0513691A (pt) * 2004-07-20 2008-03-25 Roche Diagnostics Gmbh glicose desidrogenase dependente de pirroloquinolina quinona transformada geneticamente compreendendo uma inserção de aminoácido
US7781196B2 (en) 2004-10-15 2010-08-24 Roche Diagnostics Operations, Inc. Thermostable mutants of pyrroloquinoline quinone dependent glucose dehydrogenase
US7901921B2 (en) * 2004-10-22 2011-03-08 Oncolytics Biotech Inc. Viral purification methods
WO2006085509A1 (ja) * 2005-02-08 2006-08-17 Toyo Boseki Kabushiki Kaisha 基質特異性に優れた改変型ピロロキノリンキノン依存性グルコースデヒドロゲナーゼ
EP2365073A1 (de) 2005-03-25 2011-09-14 Ikeda Food Research Co. Ltd. Coenzym-verknüpfte glucose-dehydrogenase und dafür codierendes polynucleotid
WO2006109578A1 (ja) * 2005-04-05 2006-10-19 Amano Enzyme Inc. 改変型ピロロキノリンキノン依存性グルコース脱水素酵素、及びピロロキノリンキノン依存性グルコース脱水素酵素の基質特異性改良法
JP5341312B2 (ja) 2005-06-20 2013-11-13 アークレイ株式会社 変異グルコース脱水素酵素
TW200706650A (en) * 2005-08-11 2007-02-16 Toyo Boseki A substrate specificity improved composition for glucose measurement
JP2007043984A (ja) * 2005-08-11 2007-02-22 Toyobo Co Ltd ピロロキノリンキノン依存性グルコース脱水素酵素の基質特異性を向上する方法
US7955484B2 (en) 2005-12-14 2011-06-07 Nova Biomedical Corporation Glucose biosensor and method
WO2007118647A1 (en) 2006-04-13 2007-10-25 Roche Diagnostics Gmbh Improved mutants of pyrroloquinoline quinone dependent soluble glucose dehydrogenase
KR100973992B1 (ko) * 2006-05-25 2010-08-05 삼성전자주식회사 시분할 복신 무선통신시스템의 시분할 복신 스위치
US8492130B2 (en) 2006-06-29 2013-07-23 Ikeda Food Research Co., Ltd. FAD-conjugated glucose dehydrogenase gene
US8619038B2 (en) * 2007-09-04 2013-12-31 Apple Inc. Editing interface
DE102008030435A1 (de) 2008-06-26 2010-01-07 Bayer Technology Services Gmbh Neuartige Varianten PQQ-abhängiger Glukosehydrogenase mit verbesserter Substratspezifität
FR2948680B1 (fr) 2009-07-28 2013-10-04 Centre Nat Rech Scient Nouveaux mutants de la pqq s-gdh
WO2012010308A1 (en) * 2010-07-23 2012-01-26 Roche Diagnostics Gmbh Zwitterion buffer containing compositions and uses in electroanalytical devices and methods
EP2465936A1 (de) 2010-12-20 2012-06-20 LEK Pharmaceuticals d.d. Enzymatische Synthese von Statinen und deren Zwischenprodukte
AU2011355209B2 (en) 2010-12-20 2015-09-17 Lek Pharmaceuticals D.D. Enzymatic synthesis of active pharmaceutical ingredient and intermediates thereof
WO2012084194A1 (en) 2010-12-22 2012-06-28 Roche Diagnostics Gmbh Systems and methods to compensate for sources of error during electrochemical testing
CN102559624B (zh) * 2012-03-05 2013-10-16 浙江德清汇宁生物科技有限公司 一种吡咯喹啉醌依赖型葡萄糖脱氢酶的热稳定性突变体及其高通量筛选方法
EP2636750A1 (de) 2012-03-06 2013-09-11 Roche Diagniostics GmbH Kompatibles gelöstes Ectoin sowie Derivate davon zur Enzymstabilisierung
EP2994536B1 (de) 2013-05-08 2017-06-14 Roche Diabetes Care GmbH Stabilisierung von enzymen durch nikotinsäure
WO2015078899A1 (en) 2013-11-27 2015-06-04 Roche Diagnostics Gmbh Composition comprising up-converting phosphors for detecting an analyte
EP2927319A1 (de) 2014-03-31 2015-10-07 Roche Diagnostics GmbH Hochlastenzymimmobilisierung mittels Quervernetzung
KR101896820B1 (ko) 2014-04-14 2018-09-07 에프. 호프만-라 로슈 아게 페나지늄 매개체
CN106573910B (zh) 2014-08-22 2020-08-28 豪夫迈·罗氏有限公司 氧化还原指示剂
CN111073866B (zh) * 2015-10-29 2024-03-19 英科隆生物技术（杭州）有限公司 PQQ-sGDH突变体、聚核苷酸及其在葡萄糖检测中的应用
KR102372113B1 (ko) 2016-10-05 2022-03-07 에프. 호프만-라 로슈 아게 다중 분석물 진단 테스트 엘리먼트들을 위한 검출 시약들 및 전극 배열들, 그리고 그것을 사용하는 방법들
CN113234697B (zh) * 2021-04-28 2022-09-02 遵义医科大学珠海校区 一种葡萄糖脱氢酶变体及其制备方法与应用

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6190906B1 (en) 1987-05-20 2001-02-20 Roche Diagnostics Gmbh Expression vector for the regulatable expression of foreign genes in prokaryotes
DE3716957A1 (de) 1987-05-20 1988-12-01 Boehringer Mannheim Gmbh Expressionsvektor zur regulierbaren expression von fremdgenen in prokaryonten
FI905324A (fi) 1990-10-29 1992-04-30 Valtion Teknillinen Enzymatiskt bestaemningsfoerfarande foer aldoser.
DE4311464A1 (de) 1993-04-08 1994-10-13 Boehringer Mannheim Gmbh Verfahren zur kolorimetrischen Bestimmung eines Analyten mit einer PQQ-abhängigen Dehydrogenase
DE19639169A1 (de) * 1996-09-24 1998-04-02 Boehringer Mannheim Gmbh Redoxaktive Verbindungen und deren Anwendung
JPH10243786A (ja) * 1997-03-03 1998-09-14 Koji Hayade 改変型グルコース脱水素酵素
CZ291059B6 (cs) * 1997-03-12 2002-12-11 Lonza Ag Způsob výroby esterů 2,6-pyridindikarboxylových kyselin
US5997817A (en) * 1997-12-05 1999-12-07 Roche Diagnostics Corporation Electrochemical biosensor test strip
JP2000350588A (ja) * 1999-04-08 2000-12-19 Koji Hayade グルコース脱水素酵素
TWI224136B (en) * 1999-04-30 2004-11-21 Koji Sode Glucose dehydrogenase
KR100519902B1 (ko) * 2000-10-27 2005-10-10 에프. 호프만-라 로슈 아게 가용성 피롤로퀴놀린 퀴논 의존성 글루코오스 디히드로게나아제의 변이체
JP2003093071A (ja) * 2001-09-26 2003-04-02 Koji Hayade グルコース脱水素酵素

Also Published As

Publication number Publication date
BR0114962A (pt) 2003-10-28
EP1332216A1 (de) 2003-08-06
KR100519902B1 (ko) 2005-10-10
US7132270B2 (en) 2006-11-07
JP3845618B2 (ja) 2006-11-15
HK1074217A1 (en) 2005-11-04
WO2002034919A1 (en) 2002-05-02
US20060148056A1 (en) 2006-07-06
CN1288246C (zh) 2006-12-06
PL205561B1 (pl) 2010-05-31
KR20030048447A (ko) 2003-06-19
JP2006314322A (ja) 2006-11-24
US20030104595A1 (en) 2003-06-05
BRPI0114962B8 (pt) 2021-07-27
PL362548A1 (en) 2004-11-02
CA2427029A1 (en) 2002-05-02
EP1332216B1 (de) 2011-08-17
JP2004512047A (ja) 2004-04-22
AU2002215966B2 (en) 2006-05-04
JP3889434B2 (ja) 2007-03-07
CN1578836A (zh) 2005-02-09
BRPI0114962B1 (pt) 2016-07-12
US20040005683A1 (en) 2004-01-08
CA2427029C (en) 2010-02-02
US7547535B2 (en) 2009-06-16
AU1596602A (en) 2002-05-06

Similar Documents

Publication Publication Date Title
ATE520777T1 (de) Varianten der löslichen pyrroloquinolin quinon- abhängigen glukose dehydrogenase
WO2004038401A3 (en) Biosensor having hematocrit and oxygen biases
JP2004512047A5 (de)
ES2295521T3 (es) Composiciones estabilizadoras de un mediador y procedimientos para su uso en ensayos electroquimicos de deteccion de analitos.
WO2003085372A8 (en) Biosensor membranes composed of polymers containing heterocyclic nitrogens
PL362301A1 (en) Novel glucose dehydrogenase and process for producing the dehydrogenase
EP1679508A4 (de) Biosensor und verfahren zu dessen herstellung
GB2449207B (en) Glucose dehydrogenase
Horaguchi et al. Engineering glucose oxidase to minimize the influence of oxygen on sensor response
JP2007509355A5 (de)
BRPI0513691A (pt) glicose desidrogenase dependente de pirroloquinolina quinona transformada geneticamente compreendendo uma inserção de aminoácido
TW200514851A (en) Glucose dehydrogenase/cytochrome fused protein
EP1367120A3 (de) Modifizierte pyrroloquinolin quinon-abhängigen glukose Dehydrogenase mit höher Substratspezifität und Stabilität
HK1111723A1 (en) Thermostable mutants of pyrroloquinoline quinone dependent glucose dehydrogenase
EP3303605B1 (de) Multi-mediator-reagenzformulierungen zur verwendung in der elektrochemischen detektion
NO20071564L (no) Fremgangsmate for a bestemme konsentrasjonen av analytter i prover ved direkte formidling av enzymer
AU2003242384A1 (en) Glucose dehydrogenase
EP1666586A8 (de) Pyrrolochinolin-chinon (pqq)-abhängige glucosedehydrogenase-modifikation mit ausgezeichneter substratspezifität
TW200706653A (en) A process for improving the substrate specificity of the pyrroloquinoline quinone dependency glucose dehydrogenase
JP5303461B2 (ja) グルコース脱水素酵素
EP1544292A4 (de) Glucosedehydrogenase
Cho et al. Critical residues for the coenzyme specificity of NAD+-dependent 15-hydroxyprostaglandin dehydrogenase
Kulys et al. Recombinant Microdochium nivale carbohydrate oxidase and its application in an amperometric glucose sensor
JP2004236665A (ja) Ｐｑｑ依存性グルコースデヒドロゲナーゼ組成物およびグルコース測定用試薬組成物
TW200718786A (en) Lovel kglucose dehydrogenase

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties