ATE484598T1 - Amplifikation und analyse von gesamtgenom- und gesamttranskriptom- bibliotheken, die durch ein dns-polymerisierungsverfahren produziert wurden - Google Patents

Amplifikation und analyse von gesamtgenom- und gesamttranskriptom- bibliotheken, die durch ein dns-polymerisierungsverfahren produziert wurden

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Publication number
ATE484598T1
ATE484598T1 AT04718499T AT04718499T ATE484598T1 AT E484598 T1 ATE484598 T1 AT E484598T1 AT 04718499 T AT04718499 T AT 04718499T AT 04718499 T AT04718499 T AT 04718499T AT E484598 T1 ATE484598 T1 AT E484598T1
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AT
Austria
Prior art keywords
whole
amplification
analysis
polymerization process
dna polymerization
Prior art date
Application number
AT04718499T
Other languages
German (de)
English (en)
Inventor
Emmanuel Kamberov
Tong Sun
Eric Bruening
Jon Pinter
Irina Sleptsova
Takao Kurihara
Vladimir Makarov
Original Assignee
Rubicon Genomics Inc
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Publication of ATE484598T1 publication Critical patent/ATE484598T1/de

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Analytical Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT04718499T 2003-03-07 2004-03-08 Amplifikation und analyse von gesamtgenom- und gesamttranskriptom- bibliotheken, die durch ein dns-polymerisierungsverfahren produziert wurden ATE484598T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US45306003P 2003-03-07 2003-03-07
PCT/US2004/006983 WO2004081225A2 (en) 2003-03-07 2004-03-08 Amplification and analysis of whole genome and whole transcriptome libraries generated by a dna polymerization process

Publications (1)

Publication Number Publication Date
ATE484598T1 true ATE484598T1 (de) 2010-10-15

Family

ID=32990716

Family Applications (1)

Application Number Title Priority Date Filing Date
AT04718499T ATE484598T1 (de) 2003-03-07 2004-03-08 Amplifikation und analyse von gesamtgenom- und gesamttranskriptom- bibliotheken, die durch ein dns-polymerisierungsverfahren produziert wurden

Country Status (7)

Country Link
US (2) US7718403B2 (https=)
EP (2) EP1604040B1 (https=)
JP (1) JP4773338B2 (https=)
AT (1) ATE484598T1 (https=)
DE (1) DE602004029560D1 (https=)
DK (2) DK2374900T3 (https=)
WO (1) WO2004081225A2 (https=)

Families Citing this family (178)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040024193A1 (en) * 2002-07-26 2004-02-05 Williams Gregg T. Method of detecting and quantifying hepatitis C virus
US20060183132A1 (en) * 2005-02-14 2006-08-17 Perlegen Sciences, Inc. Selection probe amplification
US20090124514A1 (en) * 2003-02-26 2009-05-14 Perlegen Sciences, Inc. Selection probe amplification
US8206913B1 (en) 2003-03-07 2012-06-26 Rubicon Genomics, Inc. Amplification and analysis of whole genome and whole transcriptome libraries generated by a DNA polymerization process
US20050181394A1 (en) * 2003-06-20 2005-08-18 Illumina, Inc. Methods and compositions for whole genome amplification and genotyping
WO2005003304A2 (en) 2003-06-20 2005-01-13 Illumina, Inc. Methods and compositions for whole genome amplification and genotyping
WO2005085477A1 (en) * 2004-03-02 2005-09-15 Orion Genomics Llc Differential enzymatic fragmentation by whole genome amplification
JP3972106B2 (ja) * 2004-03-03 2007-09-05 大学共同利用機関法人情報・システム研究機構 ゲノムライブラリー作製方法、および同方法により作製されたゲノムライブラリー
EP2380993B1 (en) 2004-03-08 2015-12-23 Rubicon Genomics, Inc. Method for generating and amplifying DNA libraries for sensitive detection and analysis of DNA methylation
ES2610781T3 (es) * 2004-09-14 2017-05-03 Argos Therapeutics, Inc. Amplificación de patógenos independiente de la cepa y vacunas para estos
EP1836302B1 (en) * 2004-12-23 2010-04-14 Amersham Biosciences Corp. Ligation-based rna amplification
US8315816B2 (en) * 2005-02-16 2012-11-20 Genetic Technologies Limited Methods of genetic analysis involving the amplification of complementary duplicons
US7449297B2 (en) * 2005-04-14 2008-11-11 Euclid Diagnostics Llc Methods of copying the methylation pattern of DNA during isothermal amplification and microarrays
EP3257949A1 (en) 2005-06-15 2017-12-20 Complete Genomics Inc. Nucleic acid analysis by random mixtures of non-overlapping fragments
US20070003938A1 (en) * 2005-06-30 2007-01-04 Perlegen Sciences, Inc. Hybridization of genomic nucleic acid without complexity reduction
WO2007018601A1 (en) 2005-08-02 2007-02-15 Rubicon Genomics, Inc. Compositions and methods for processing and amplification of dna, including using multiple enzymes in a single reaction
WO2007018602A1 (en) * 2005-08-02 2007-02-15 Rubicon Genomics, Inc. Isolation of cpg islands by thermal segregation and enzymatic selection-amplification method
US20090137415A1 (en) * 2005-08-05 2009-05-28 Euclid Diagnostics Llc SUBTRACTIVE SEPARATION AND AMPLIFICATION OF NON-RIBOSOMAL TRANSCRIBED RNA (nrRNA)
JP2009508495A (ja) * 2005-09-16 2009-03-05 ４５４ ライフ サイエンシーズ コーポレイション ｃＤＮＡライブラリー調製
US9134237B2 (en) * 2005-09-20 2015-09-15 Janssen Diagnotics, LLC High sensitivity multiparameter method for rare event analysis in a biological sample
CA2623405C (en) * 2005-09-20 2014-11-25 Immunivest Corporation Methods and composition to generate unique sequence dna probes labeling of dna probes and the use of these probes
US20070092899A1 (en) * 2005-10-21 2007-04-26 The Regents Of The University Of California Multiple displacement amplification with blocker DNA
JP2009514555A (ja) 2005-11-08 2009-04-09 ユークリッド ダイアグノスティックス リミテッド ライアビリティー カンパニー 癌評価の遺伝子関連ＣｐＧアイランドのメチル化アッセイのための材料と方法
US20080057499A1 (en) * 2006-02-06 2008-03-06 Affymetrix, Inc. Methods for high specificity whole genome amplification and hybridization
DK2002016T3 (da) * 2006-04-12 2010-03-29 Medical Res Council Fremgangsmåde til bestemmelse af kopital
WO2007136874A2 (en) * 2006-05-18 2007-11-29 President And Fellows Of Harvard College Genomic library construction
WO2009035447A1 (en) * 2006-06-14 2009-03-19 Living Microsystems, Inc. Diagnosis of fetal abnormalities by comparative genomic hybridization analysis
US9051601B2 (en) 2006-08-01 2015-06-09 Gen-Probe Incorporated Methods of nonspecific target capture of nucleic acids
US9149473B2 (en) * 2006-09-14 2015-10-06 Ibis Biosciences, Inc. Targeted whole genome amplification method for identification of pathogens
ES2582602T3 (es) * 2007-03-28 2016-09-14 Signal Diagnostics Sistema y método de análisis de alta resolución de ácidos nucleicos para detectar variaciones de secuencia
US7794985B2 (en) * 2007-04-04 2010-09-14 Ghc Technologies, Inc. Methods and compositions for rapid amplification, capture and detection of nucleic acids and proteins
JP2010528608A (ja) * 2007-06-01 2010-08-26 ４５４ ライフ サイエンシーズ コーポレイション 複合的な混合物から個々の試料を特定するためのシステムおよび方法
US8221981B2 (en) * 2007-07-30 2012-07-17 Argos Therapeutics, Inc. Primers and probes for the amplification and detection of HIV Gag, Rev and Nef polynucleotides
EP2198042B1 (en) 2007-09-17 2016-11-02 MDxHealth SA Novel markers for bladder cancer detection
US20090099040A1 (en) * 2007-10-15 2009-04-16 Sigma Aldrich Company Degenerate oligonucleotides and their uses
US8592150B2 (en) 2007-12-05 2013-11-26 Complete Genomics, Inc. Methods and compositions for long fragment read sequencing
US20090291475A1 (en) * 2008-04-23 2009-11-26 Kai Qin Lao Sequence amplification with linear primers
WO2009148560A2 (en) * 2008-05-30 2009-12-10 Board Of Regents, The Universtiy Of Texas System Methods and compositions for nucleic acid sequencing
US20100028955A1 (en) * 2008-07-22 2010-02-04 Life Technologies Corporation Sequence Amplification with Target Primers
JP5652843B2 (ja) * 2008-10-17 2015-01-14 独立行政法人農業・食品産業技術総合研究機構 Ｄｎａ増幅法
US10669574B2 (en) 2008-11-18 2020-06-02 XCR Diagnostics, Inc. DNA amplification technology
WO2010080691A1 (en) * 2009-01-06 2010-07-15 Qimin You Cross priming amplification of target nucleic acids
CA2755358A1 (en) 2009-03-13 2010-09-16 Mdxhealth Sa Novel markers for bladder cancer detection
US9524369B2 (en) * 2009-06-15 2016-12-20 Complete Genomics, Inc. Processing and analysis of complex nucleic acid sequence data
FR2949120A1 (fr) * 2009-08-13 2011-02-18 Centre Nat Rech Scient Procede de detection d'un adn circularise et utilisation de ce procede pour la detection de mutations
WO2011044437A2 (en) * 2009-10-09 2011-04-14 Stc.Unm Polony sequencing methods
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
US20110195457A1 (en) * 2010-02-09 2011-08-11 General Electric Company Isothermal amplification of nucleic acid using primers comprising a randomized sequence and specific primers and uses thereof
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
DK201000356A (en) * 2010-04-22 2011-10-23 Statens Seruminstitut New nucleotide sequence amplification Method
KR101334072B1 (ko) * 2010-11-25 2013-11-29 한국표준과학연구원 핵산 정량 방법 및 키트
US9738930B2 (en) 2011-01-28 2017-08-22 The Broad Institute, Inc. Paired end bead amplification and high throughput sequencing
US9290815B2 (en) 2011-02-21 2016-03-22 Qiagen Gmbh Method for quantifying human DNA
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
EP2714938B1 (en) * 2011-05-27 2017-11-15 President and Fellows of Harvard College Methods of amplifying whole genome of a single cell
US10364464B2 (en) 2011-08-08 2019-07-30 The Broad Institute, Inc. Compositions and methods for co-amplifying subsequences of a nucleic acid fragment sequence
WO2013101783A2 (en) 2011-12-30 2013-07-04 Bio-Rad Laboratories, Inc. Methods and compositions for performing nucleic acid amplification reactions
CA2865575C (en) 2012-02-27 2024-01-16 Cellular Research, Inc. Compositions and kits for molecular counting
LT2825672T (lt) * 2012-03-13 2019-06-10 Swift Biosciences, Inc. Būdai ir kompozicijos, skirti valdomam pagal dydį substrato polinukleotidų homopolimeriniam uodegų formavimui su nukleorūgščių polimeraze
WO2013143133A1 (zh) * 2012-03-30 2013-10-03 深圳华大基因科技服务有限公司 全基因组扩增方法及其应用
US20150141258A1 (en) * 2012-04-30 2015-05-21 Qiagen Gmbh Targeted dna enrichment and sequencing
US10870099B2 (en) 2012-07-26 2020-12-22 Illumina, Inc. Compositions and methods for the amplification of nucleic acids
WO2014043140A1 (en) * 2012-09-12 2014-03-20 The Regents Of The University Of California Accurate genome sequencing of single cells by single-stranded amplification and sequencing
EP4592400A3 (en) 2012-10-17 2025-10-29 10x Genomics Sweden AB Methods and product for optimising localised or spatial detection of gene expression in a tissue sample
EP2722399A1 (en) * 2012-10-18 2014-04-23 Roche Diagniostics GmbH Method for preventing high molecular weight products during amplification
KR101451874B1 (ko) * 2013-02-27 2014-10-16 국민대학교산학협력단 실시간 ｐｃｒ을 이용한 미생물의 최단시간 검출방법
CA2916662C (en) 2013-06-25 2022-03-08 Prognosys Biosciences, Inc. Methods and systems for determining spatial patterns of biological targets in a sample
KR102758333B1 (ko) 2013-08-28 2025-01-23 벡톤 디킨슨 앤드 컴퍼니 대량의 동시 단일 세포 분석
KR102323375B1 (ko) 2013-10-09 2021-11-08 플루어레센트릭 인코포레이티드 다중 프로브
CN105917002A (zh) 2013-11-18 2016-08-31 鲁比康基因组学公司 用于减少背景的可降解适体
US9293316B2 (en) 2014-04-04 2016-03-22 Thermo Finnigan Llc Ion separation and storage system
CN105463585B (zh) * 2014-09-12 2018-04-20 清华大学 基于单链dna分子构建测序文库的方法及其应用
EP3208343B1 (en) * 2014-10-13 2022-01-05 MGI Tech Co., Ltd. Nucleic acid fragmentation method and sequence combination
CN106715714B (zh) * 2014-10-17 2021-11-09 深圳华大智造科技股份有限公司 一种用于核酸随机片段化的引物及核酸随机片段化方法
US10968536B2 (en) 2015-02-25 2021-04-06 Jumpcode Genomics, Inc. Methods and compositions for sequencing
WO2016138496A1 (en) 2015-02-27 2016-09-01 Cellular Research, Inc. Spatially addressable molecular barcoding
WO2016160844A2 (en) 2015-03-30 2016-10-06 Cellular Research, Inc. Methods and compositions for combinatorial barcoding
US10301660B2 (en) 2015-03-30 2019-05-28 Takara Bio Usa, Inc. Methods and compositions for repair of DNA ends by multiple enzymatic activities
WO2016156529A1 (en) * 2015-03-31 2016-10-06 Qiagen Gmbh Efficiency improving ligation methods
EP3901282B1 (en) 2015-04-10 2023-06-28 Spatial Transcriptomics AB Spatially distinguished, multiplex nucleic acid analysis of biological specimens
EP4582556A3 (en) 2015-04-23 2025-10-08 Becton, Dickinson and Company Methods and compositions for whole transcriptome amplification
CA2983819A1 (en) * 2015-04-24 2016-10-27 Atila Biosystems Incorporated Amplification with primers of limited nucleotide composition
MX2018000729A (es) 2015-07-17 2018-09-06 Harvard College Métodos para amplificar las secuencias de ácido nucleico.
EP3332024B1 (en) * 2015-08-06 2020-10-28 F.Hoffmann-La Roche Ag Target enrichment by single probe primer extension
CN105602939A (zh) * 2015-09-02 2016-05-25 序康医疗科技（苏州）有限公司 扩增dna的方法
JP6940484B2 (ja) 2015-09-11 2021-09-29 セルラー リサーチ， インコーポレイテッド ライブラリー正規化のための方法および組成物
CN105506125B (zh) * 2016-01-12 2019-01-22 上海美吉生物医药科技有限公司 一种dna的测序方法及一种二代测序文库
US11339427B2 (en) 2016-02-12 2022-05-24 Jumpcode Genomics, Inc. Method for target specific RNA transcription of DNA sequences
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
JP7343264B2 (ja) * 2016-06-29 2023-09-12 トヨタ自動車株式会社 Ｄｎａライブラリーの作製方法及びｄｎａライブラリーを用いたゲノムｄｎａ解析方法
WO2018003220A1 (ja) * 2016-06-29 2018-01-04 トヨタ自動車株式会社 Ｄｎａライブラリーの作製方法及びｄｎａライブラリーを用いたゲノムｄｎａ解析方法
JP6889769B2 (ja) * 2016-07-18 2021-06-18 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 核酸配列決定の非対称な鋳型および非対称な方法
RU2736351C2 (ru) * 2016-08-31 2020-11-16 Президент Энд Фэллоуз Оф Харвард Коллидж Способы дискретной амплификации полного генома
CN110012671B (zh) 2016-09-06 2024-05-28 合成Dna技术公司 Ngs文库浓度的标准化
CN109791157B (zh) 2016-09-26 2022-06-07 贝克顿迪金森公司 使用具有条形码化的寡核苷酸序列的试剂测量蛋白质表达
EP3521445A4 (en) 2016-09-30 2019-10-09 FUJIFILM Corporation METHOD FOR DETECTING BASE SEQUENCE INFORMATION ON INDIVIDUAL CELLS DERIVED FROM SWIVELING
CN110382708A (zh) 2017-02-01 2019-10-25 赛卢拉研究公司 使用阻断性寡核苷酸进行选择性扩增
JP7056012B2 (ja) 2017-05-19 2022-04-19 トヨタ自動車株式会社 ランダムプライマーセット、及びこれを用いたｄｎａライブラリーの作製方法
CN110719959B (zh) 2017-06-05 2021-08-06 贝克顿迪金森公司 针对单细胞的样品索引
WO2019018561A1 (en) * 2017-07-19 2019-01-24 The Scripps Research Institute GENOMIC LIBRARY GENERATION IN SOLID PHASE FOR HIGH FLOW SEQUENCING
WO2019033065A1 (en) 2017-08-11 2019-02-14 Atila Biosystems, Inc. DIGITAL AMPLIFICATION WITH LIMITED NUCLEOTIDE COMPOSITION PRIMERS
JP2021505157A (ja) * 2017-12-07 2021-02-18 マサチューセッツ インスティテュート オブ テクノロジー 単一細胞分析
CN109971843B (zh) * 2017-12-27 2022-11-11 复旦大学泰州健康科学研究院 一种单细胞转录组的测序方法
KR102933367B1 (ko) 2018-02-27 2026-03-03 코넬 유니버시티 게놈-와이드 통합을 통한 순환 종양 dna의 초민감 검출
CN112272710A (zh) 2018-05-03 2021-01-26 贝克顿迪金森公司 高通量多组学样品分析
JP7358388B2 (ja) 2018-05-03 2023-10-10 ベクトン・ディキンソン・アンド・カンパニー 反対側の転写物末端における分子バーコーディング
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
ES2992135T3 (es) 2018-10-01 2024-12-09 Becton Dickinson Co Determinar secuencias de transcripción 5’
US11932849B2 (en) 2018-11-08 2024-03-19 Becton, Dickinson And Company Whole transcriptome analysis of single cells using random priming
US12529094B2 (en) 2018-12-10 2026-01-20 10X Genomics, Inc. Imaging system hardware
WO2020123316A2 (en) 2018-12-10 2020-06-18 10X Genomics, Inc. Methods for determining a location of a biological analyte in a biological sample
US11492660B2 (en) 2018-12-13 2022-11-08 Becton, Dickinson And Company Selective extension in single cell whole transcriptome analysis
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
ES2945227T3 (es) 2019-01-23 2023-06-29 Becton Dickinson Co Oligonucleótidos asociados con anticuerpos
WO2020167920A1 (en) 2019-02-14 2020-08-20 Cellular Research, Inc. Hybrid targeted and whole transcriptome amplification
US20210381035A1 (en) * 2019-02-15 2021-12-09 Takara Bio Usa, Inc. Methods of Preparing and Analyzing Nucleic Acid Libraries
WO2020191521A1 (zh) * 2019-03-22 2020-10-01 深圳华大智造科技有限公司 核酸序列、rna目标区域测序文库的构建方法及应用
WO2020243579A1 (en) 2019-05-30 2020-12-03 10X Genomics, Inc. Methods of detecting spatial heterogeneity of a biological sample
EP3994262A4 (en) * 2019-06-26 2023-09-20 Stratos Genomics Inc. IMPROVEMENT OF THE POLYMERIZATION OF NUCLEIC ACIDS BY AROMATIC COMPOUNDS
CN120099137A (zh) 2019-07-22 2025-06-06 贝克顿迪金森公司 单细胞染色质免疫沉淀测序测定
GB201911515D0 (en) * 2019-08-12 2019-09-25 Univ London Queen Mary Methods for generating a population of polynucleotide molecules
WO2021092433A2 (en) 2019-11-08 2021-05-14 10X Genomics, Inc. Enhancing specificity of analyte binding
JP7522189B2 (ja) 2019-11-08 2024-07-24 ベクトン・ディキンソン・アンド・カンパニー 免疫レパートリーシーケンシングのための完全長ｖ（ｄ）ｊ情報を得るためのランダムプライミングの使用
WO2021127406A1 (en) * 2019-12-19 2021-06-24 The Regents Of The University Of California Methods of producing target capture nucleic acids
ES2982420T3 (es) 2019-12-23 2024-10-16 10X Genomics Inc Métodos para el análisis espacial mediante el uso de la ligazón con plantilla de ARN
EP4081656A1 (en) 2019-12-23 2022-11-02 10X Genomics, Inc. Compositions and methods for using fixed biological samples in partition-based assays
CN115135984B (zh) 2019-12-23 2025-12-23 10X基因组学有限公司 可逆固定试剂及其使用方法
WO2021146219A1 (en) 2020-01-13 2021-07-22 Becton, Dickinson And Company Cell capture using du-containing oligonucleotides
US12365942B2 (en) 2020-01-13 2025-07-22 10X Genomics, Inc. Methods of decreasing background on a spatial array
US11649497B2 (en) 2020-01-13 2023-05-16 Becton, Dickinson And Company Methods and compositions for quantitation of proteins and RNA
US12405264B2 (en) 2020-01-17 2025-09-02 10X Genomics, Inc. Electrophoretic system and method for analyte capture
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US20210230681A1 (en) 2020-01-24 2021-07-29 10X Genomics, Inc. Methods for spatial analysis using proximity ligation
WO2021155057A1 (en) 2020-01-29 2021-08-05 Becton, Dickinson And Company Barcoded wells for spatial mapping of single cells through sequencing
US12076701B2 (en) 2020-01-31 2024-09-03 10X Genomics, Inc. Capturing oligonucleotides in spatial transcriptomics
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US12110541B2 (en) 2020-02-03 2024-10-08 10X Genomics, Inc. Methods for preparing high-resolution spatial arrays
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
WO2021158925A1 (en) 2020-02-07 2021-08-12 10X Genomics, Inc. Quantitative and automated permeabilization performance evaluation for spatial transcriptomics
US12281357B1 (en) 2020-02-14 2025-04-22 10X Genomics, Inc. In situ spatial barcoding
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
WO2021173719A1 (en) 2020-02-25 2021-09-02 Becton, Dickinson And Company Bi-specific probes to enable the use of single-cell samples as single color compensation control
EP4139485B1 (en) 2020-04-22 2023-09-06 10X Genomics, Inc. Methods for spatial analysis using targeted rna depletion
US11702689B2 (en) * 2020-04-24 2023-07-18 Microsoft Technology Licensing, Llc Homopolymer primers for amplification of polynucleotides created by enzymatic synthesis
US11661625B2 (en) 2020-05-14 2023-05-30 Becton, Dickinson And Company Primers for immune repertoire profiling
EP4153776B1 (en) 2020-05-22 2025-03-05 10X Genomics, Inc. Spatial analysis to detect sequence variants
EP4414459B1 (en) 2020-05-22 2025-09-03 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
US12157913B2 (en) 2020-06-02 2024-12-03 Becton, Dickinson And Company Oligonucleotides and beads for 5 prime gene expression assay
US12031177B1 (en) 2020-06-04 2024-07-09 10X Genomics, Inc. Methods of enhancing spatial resolution of transcripts
EP4421186B1 (en) 2020-06-08 2025-08-13 10X Genomics, Inc. Methods of determining a surgical margin and methods of use thereof
ES2994976T3 (en) 2020-06-25 2025-02-05 10X Genomics Inc Spatial analysis of dna methylation
US12209280B1 (en) 2020-07-06 2025-01-28 10X Genomics, Inc. Methods of identifying abundance and location of an analyte in a biological sample using second strand synthesis
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
US11981960B1 (en) 2020-07-06 2024-05-14 10X Genomics, Inc. Spatial analysis utilizing degradable hydrogels
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
US12391940B2 (en) 2020-07-31 2025-08-19 Becton, Dickinson And Company Single cell assay for transposase-accessible chromatin
US11981958B1 (en) 2020-08-20 2024-05-14 10X Genomics, Inc. Methods for spatial analysis using DNA capture
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
EP4247967A1 (en) 2020-11-20 2023-09-27 Becton, Dickinson and Company Profiling of highly expressed and lowly expressed proteins
CN116685850A (zh) 2020-12-15 2023-09-01 贝克顿迪金森公司 单细胞分泌组分析
EP4729631A2 (en) 2020-12-21 2026-04-22 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
US11280028B1 (en) * 2021-02-24 2022-03-22 Agency For Science, Technology And Research (A*Star) Unbiased and simultaneous amplification method for preparing a double-stranded DNA library from a sample of more than one type of nucleic acid
EP4305196B1 (en) 2021-04-14 2025-04-02 10X Genomics, Inc. Methods of measuring mislocalization of an analyte
EP4320271B1 (en) 2021-05-06 2025-03-19 10X Genomics, Inc. Methods for increasing resolution of spatial analysis
WO2022256503A1 (en) 2021-06-03 2022-12-08 10X Genomics, Inc. Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis
WO2023034489A1 (en) 2021-09-01 2023-03-09 10X Genomics, Inc. Methods, compositions, and kits for blocking a capture probe on a spatial array
WO2023086880A1 (en) * 2021-11-10 2023-05-19 10X Genomics, Inc. Methods, compositions, and kits for determining the location of an analyte in a biological sample
WO2023102118A2 (en) 2021-12-01 2023-06-08 10X Genomics, Inc. Methods, compositions, and systems for improved in situ detection of analytes and spatial analysis
WO2023147568A2 (en) * 2022-01-28 2023-08-03 Flagship Pioneering Innovations Vi, Llc Compositions and methods for making and using an immortalized library
DE102022211087A1 (de) * 2022-10-19 2024-04-25 Robert Bosch Gesellschaft mit beschränkter Haftung Verfahren und Vorrichtung zur Voramplifikation eines Nukleinsäuregemisches
EP4482979B1 (en) 2022-11-09 2025-10-01 10X Genomics, Inc. Methods, compositions, and kits for determining the location of multiple analytes in a biological sample
EP4628594A1 (en) * 2024-04-05 2025-10-08 4basebio UK Ltd Methods of amplifying dna and dna products with inhibitory regions

Family Cites Families (73)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4883750A (en) 1984-12-13 1989-11-28 Applied Biosystems, Inc. Detection of specific sequences in nucleic acids
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4800159A (en) 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
AU622104B2 (en) 1987-03-11 1992-04-02 Sangtec Molecular Diagnostics Ab Method of assaying of nucleic acids, a reagent combination and kit therefore
IL86724A (en) 1987-06-19 1995-01-24 Siska Diagnostics Inc Methods and kits for amplification and testing of nucleic acid sequences
CA1323293C (en) 1987-12-11 1993-10-19 Keith C. Backman Assay using template-dependent nucleic acid probe reorganization
ATE92538T1 (de) 1988-01-21 1993-08-15 Genentech Inc Verstaerkung und nachweis von nukleinsaeuresequenzen.
US6107023A (en) 1988-06-17 2000-08-22 Genelabs Technologies, Inc. DNA amplification and subtraction techniques
EP0426756A4 (en) 1988-07-26 1991-11-21 Genelabs Incorporated Rna and dna amplification techniques
US4932207A (en) 1988-12-28 1990-06-12 Sundstrand Corporation Segmented seal plate for a turbine engine
US5043272A (en) 1989-04-27 1991-08-27 Life Technologies, Incorporated Amplification of nucleic acid sequences using oligonucleotides of random sequence as primers
US5106727A (en) 1989-04-27 1992-04-21 Life Technologies, Inc. Amplification of nucleic acid sequences using oligonucleotides of random sequences as primers
US6040138A (en) * 1995-09-15 2000-03-21 Affymetrix, Inc. Expression monitoring by hybridization to high density oligonucleotide arrays
US5104792A (en) 1989-12-21 1992-04-14 The United States Of America As Represented By The Department Of Health And Human Services Method for amplifying unknown nucleic acid sequences
AU672760B2 (en) 1991-09-24 1996-10-17 Keygene N.V. Selective restriction fragment amplification: a general method for DNA fingerprinting
WO1993014217A1 (en) * 1992-01-10 1993-07-22 Life Technologies, Inc. Use of predetermined nucleotides having altered base pairing characteristics in the amplification of nucleic acid molecules
KR100249110B1 (ko) * 1992-05-06 2000-04-01 다니엘 엘. 캐시앙 핵산 서열 증폭 방법, 이에 이용되는 조성물 및 키트
US5514545A (en) 1992-06-11 1996-05-07 Trustees Of The University Of Pennsylvania Method for characterizing single cells based on RNA amplification for diagnostics and therapeutics
US5731171A (en) * 1993-07-23 1998-03-24 Arch Development Corp. Sequence independent amplification of DNA
US5523204A (en) * 1993-12-10 1996-06-04 Becton Dickinson And Company Detection of nucleic acids in cells by strand displacement amplification
US5710000A (en) 1994-09-16 1998-01-20 Affymetrix, Inc. Capturing sequences adjacent to Type-IIs restriction sites for genomic library mapping
GB9422891D0 (en) 1994-11-12 1995-01-04 Univ Nottingham Improvements in and relating to the amplification and identification of nucleotide sequences
JPH08173164A (ja) 1994-12-22 1996-07-09 Hitachi Ltd Ｄｎａ調製法
US5565340A (en) 1995-01-27 1996-10-15 Clontech Laboratories, Inc. Method for suppressing DNA fragment amplification during PCR
US5994058A (en) 1995-03-20 1999-11-30 Genome International Corporation Method for contiguous genome sequencing
US5871820A (en) 1995-04-06 1999-02-16 General Electric Company Protection of thermal barrier coating with an impermeable barrier coating
US5750341A (en) * 1995-04-17 1998-05-12 Lynx Therapeutics, Inc. DNA sequencing by parallel oligonucleotide extensions
US5648245A (en) 1995-05-09 1997-07-15 Carnegie Institution Of Washington Method for constructing an oligonucleotide concatamer library by rolling circle replication
US5814444A (en) 1995-06-07 1998-09-29 University Of Washington Methods for making and using single-chromosome amplfication libraries
EP0951541B1 (en) 1995-07-31 2005-11-30 Urocor, Inc. Biomarkers and targets for diagnosis, prognosis and management of prostate disease
US5631147A (en) * 1995-09-21 1997-05-20 Becton, Dickinson And Company Detection of nucleic acids in cells by thermophilic strand displacement amplification
EP0880598A4 (en) * 1996-01-23 2005-02-23 Affymetrix Inc RAPID EVALUATION OF NUCLEIC ACID ABUNDANCE DIFFERENCE, WITH A HIGH-DENSITY OLIGONUCLEOTIDE SYSTEM
ATE284891T1 (de) 1996-02-14 2005-01-15 Biomerieux Bv Isolierung von einzelsträngigen nukleinsäuren
EP0910666B1 (en) * 1996-05-02 2006-08-09 Applera Corporation Quantitation of rna transcripts using genomic dna as the internal amplification competitor
CA2234736A1 (en) 1996-07-16 1998-01-22 Periannan Senapathy Method for contiguous genome sequencing
EP0835935A1 (en) * 1996-10-03 1998-04-15 Roche Diagnostics GmbH Thermostable DNA Polymerase from Anaerocellum thermophilum
US6030814A (en) * 1997-04-21 2000-02-29 Epicentre Technologies Corporation Reverse transcription method
US6124120A (en) 1997-10-08 2000-09-26 Yale University Multiple displacement amplification
US5932451A (en) 1997-11-19 1999-08-03 Incyte Pharmaceuticals, Inc. Method for unbiased mRNA amplification
DE19813317A1 (de) 1998-03-26 1999-09-30 Roche Diagnostics Gmbh Verbessertes Verfahren zur Primer Extension Präamplifikations-PCR
US6232067B1 (en) * 1998-08-17 2001-05-15 The Perkin-Elmer Corporation Adapter directed expression analysis
IL141749A0 (en) 1998-09-18 2002-03-10 Micromet Ag Dna amplification of a single cell
US6506594B1 (en) * 1999-03-19 2003-01-14 Cornell Res Foundation Inc Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
US6521428B1 (en) 1999-04-21 2003-02-18 Genome Technologies, Llc Shot-gun sequencing and amplification without cloning
US6491752B1 (en) 1999-07-16 2002-12-10 Sumco Oregon Corporation Enhanced n-type silicon material for epitaxial wafer substrate and method of making same
AU6387000A (en) 1999-07-29 2001-02-19 Genzyme Corporation Serial analysis of genetic alterations
US6846626B1 (en) * 1999-09-01 2005-01-25 Genome Technologies, Llc Method for amplifying sequences from unknown DNA
US6692918B2 (en) 1999-09-13 2004-02-17 Nugen Technologies, Inc. Methods and compositions for linear isothermal amplification of polynucleotide sequences
US6271002B1 (en) 1999-10-04 2001-08-07 Rosetta Inpharmatics, Inc. RNA amplification method
US6958225B2 (en) 1999-10-27 2005-10-25 Affymetrix, Inc. Complexity management of genomic DNA
EP1276901A2 (en) * 2000-01-13 2003-01-22 Amsterdam Support Diagnostics B.V. A universal nucleic acid amplification system for nucleic acids in a sample
US6379932B1 (en) 2000-07-17 2002-04-30 Incyte Genomics, Inc. Single primer PCR amplification of RNA
US6713257B2 (en) * 2000-08-25 2004-03-30 Rosetta Inpharmatics Llc Gene discovery using microarrays
WO2002020843A1 (en) * 2000-09-06 2002-03-14 Rutgers, The State University A method for sequencing nucleic acid sequences without chain termination
EP1315799A2 (en) 2000-09-08 2003-06-04 bioMérieux BV Attenuated hiv strains and use thereof
US6794141B2 (en) 2000-12-22 2004-09-21 Arcturus Bioscience, Inc. Nucleic acid amplification
WO2002061140A2 (en) * 2001-01-31 2002-08-08 Ambion, Inc. Competitive population normalization for comparative analysis of nucleic acid samples
WO2002065093A2 (en) 2001-02-14 2002-08-22 Baylor College Of Medicine Methods and compositions of amplifying rna
MXPA02012739A (es) 2001-03-09 2004-04-20 Nugen Technologies Inc Metodos y composiciones para amplificacion de secuencias de arn.
WO2002103054A1 (en) 2001-05-02 2002-12-27 Rubicon Genomics Inc. Genome walking by selective amplification of nick-translate dna library and amplification from complex mixtures of templates
JP2002345466A (ja) 2001-05-08 2002-12-03 Takara Bio Inc Ｄｎａの増幅方法
US6638722B2 (en) 2001-06-13 2003-10-28 Invitrogen Corporation Method for rapid amplification of DNA
EP1275738A1 (en) 2001-07-11 2003-01-15 Roche Diagnostics GmbH Method for random cDNA synthesis and amplification
EP1275734A1 (en) 2001-07-11 2003-01-15 Roche Diagnostics GmbH Method for random cDNA synthesis and amplification
US20040175715A1 (en) 2001-08-21 2004-09-09 Burgoyne Leigh A. Method and device for simultaneously molecularly cloning and polylocus profiling of genomes or genomes mixtures
US20040019005A1 (en) * 2001-10-01 2004-01-29 Jeffrey Van Ness Methods for parallel measurement of genetic variations
JP2005535283A (ja) 2001-11-13 2005-11-24 ルビコン ゲノミクス インコーポレイテッド ランダムフラグメント化により生成されたｄｎａ分子を用いたｄｎａ増幅および配列決定
US20030211528A1 (en) * 2002-03-12 2003-11-13 Norman Iscove Exponential amplification of sub-picogram nucleic acid samples with retention of quantitative representation
KR100545295B1 (ko) * 2003-09-29 2006-01-24 현대모비스 주식회사 차량용 조향장치의 너클 어셈블리
EP2380993B1 (en) 2004-03-08 2015-12-23 Rubicon Genomics, Inc. Method for generating and amplifying DNA libraries for sensitive detection and analysis of DNA methylation
US8312249B1 (en) 2008-10-10 2012-11-13 Apple Inc. Dynamic trampoline and structured code generation in a signed code environment
FR2957821B1 (fr) 2010-03-24 2014-08-29 Inst Francais Du Petrole Nouvelle zone de regeneration du catalyseur divisee en secteurs pour unites catalytiques regeneratives

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