ATE428805T1 - Verfahren zur fehlerreduzierung in nukleinsäurepopulationen - Google Patents
Verfahren zur fehlerreduzierung in nukleinsäurepopulationenInfo
- Publication number
- ATE428805T1 ATE428805T1 AT03711383T AT03711383T ATE428805T1 AT E428805 T1 ATE428805 T1 AT E428805T1 AT 03711383 T AT03711383 T AT 03711383T AT 03711383 T AT03711383 T AT 03711383T AT E428805 T1 ATE428805 T1 AT E428805T1
- Authority
- AT
- Austria
- Prior art keywords
- dna
- segments
- microarray
- substrate
- duplexes
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 2
- 150000007523 nucleic acids Chemical class 0.000 title 1
- 102000039446 nucleic acids Human genes 0.000 title 1
- 108020004707 nucleic acids Proteins 0.000 title 1
- 108020004414 DNA Proteins 0.000 abstract 9
- 102000053602 DNA Human genes 0.000 abstract 2
- 230000015572 biosynthetic process Effects 0.000 abstract 2
- 238000002493 microarray Methods 0.000 abstract 2
- 239000000758 substrate Substances 0.000 abstract 2
- 238000003786 synthesis reaction Methods 0.000 abstract 2
- 238000000018 DNA microarray Methods 0.000 abstract 1
- 230000004568 DNA-binding Effects 0.000 abstract 1
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 1
- 239000011230 binding agent Substances 0.000 abstract 1
- 230000000295 complement effect Effects 0.000 abstract 1
- 238000009396 hybridization Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
- C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
- C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Plant Pathology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Immunology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Paper (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US36056302P | 2002-02-28 | 2002-02-28 | |
| PCT/US2003/006500 WO2003072832A1 (en) | 2002-02-28 | 2003-02-28 | Method of error reduction in nucleic acid populations |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE428805T1 true ATE428805T1 (de) | 2009-05-15 |
Family
ID=27766234
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT03711383T ATE428805T1 (de) | 2002-02-28 | 2003-02-28 | Verfahren zur fehlerreduzierung in nukleinsäurepopulationen |
Country Status (12)
| Country | Link |
|---|---|
| US (2) | US7303872B2 (de) |
| EP (1) | EP1483409B1 (de) |
| JP (1) | JP4455061B2 (de) |
| KR (1) | KR101026816B1 (de) |
| AT (1) | ATE428805T1 (de) |
| AU (1) | AU2003213696B2 (de) |
| CA (1) | CA2477519C (de) |
| DE (1) | DE60327183D1 (de) |
| IL (2) | IL163788A0 (de) |
| IS (1) | IS7430A (de) |
| MX (1) | MXPA04008373A (de) |
| WO (1) | WO2003072832A1 (de) |
Families Citing this family (27)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2002095073A1 (en) | 2001-05-18 | 2002-11-28 | Wisconsin Alumni Research Foundation | Method for the synthesis of dna sequences |
| ATE428805T1 (de) * | 2002-02-28 | 2009-05-15 | Wisconsin Alumni Res Found | Verfahren zur fehlerreduzierung in nukleinsäurepopulationen |
| US7563600B2 (en) | 2002-09-12 | 2009-07-21 | Combimatrix Corporation | Microarray synthesis and assembly of gene-length polynucleotides |
| US8133670B2 (en) * | 2003-06-13 | 2012-03-13 | Cold Spring Harbor Laboratory | Method for making populations of defined nucleic acid molecules |
| US8105780B2 (en) * | 2003-09-22 | 2012-01-31 | Agency For Science, Technology And Research | Device and method of detecting mutations and polymorphisms in DNA |
| AU2005222788A1 (en) * | 2004-02-27 | 2005-09-29 | President And Fellows Of Harvard College | Polynucleotide synthesis |
| EP1574570A1 (de) * | 2004-03-12 | 2005-09-14 | Universität Regensburg | Verfahren zur Verringerung der Anzahl von fehlgepaarten Nukleotiden in doppelsträngigen Polynukleotiden |
| US20100016178A1 (en) * | 2004-06-09 | 2010-01-21 | Sussman Michael R | Methods for rapid production of target double-stranded dna sequences |
| WO2006026614A2 (en) * | 2004-08-27 | 2006-03-09 | Wisconsin Alumni Research Foundation | Method of error reduction in nucleic acid populations |
| US20070122817A1 (en) * | 2005-02-28 | 2007-05-31 | George Church | Methods for assembly of high fidelity synthetic polynucleotides |
| US20070269870A1 (en) * | 2004-10-18 | 2007-11-22 | George Church | Methods for assembly of high fidelity synthetic polynucleotides |
| US7722824B2 (en) | 2005-08-02 | 2010-05-25 | Wisconsin Alumni Research Foundation | Synthesis of arrays of oligonucleotides and other chain molecules |
| US20070196834A1 (en) * | 2005-09-09 | 2007-08-23 | Francesco Cerrina | Method and system for the generation of large double stranded DNA fragments |
| US20070231805A1 (en) * | 2006-03-31 | 2007-10-04 | Baynes Brian M | Nucleic acid assembly optimization using clamped mismatch binding proteins |
| US20090087840A1 (en) * | 2006-05-19 | 2009-04-02 | Codon Devices, Inc. | Combined extension and ligation for nucleic acid assembly |
| US8053191B2 (en) | 2006-08-31 | 2011-11-08 | Westend Asset Clearinghouse Company, Llc | Iterative nucleic acid assembly using activation of vector-encoded traits |
| WO2011056872A2 (en) | 2009-11-03 | 2011-05-12 | Gen9, Inc. | Methods and microfluidic devices for the manipulation of droplets in high fidelity polynucleotide assembly |
| ES2574055T3 (es) | 2009-11-25 | 2016-06-14 | Gen9, Inc. | Métodos y aparatos para la reducción de errores en el ADN basada en un chip |
| WO2011066185A1 (en) | 2009-11-25 | 2011-06-03 | Gen9, Inc. | Microfluidic devices and methods for gene synthesis |
| US9217144B2 (en) | 2010-01-07 | 2015-12-22 | Gen9, Inc. | Assembly of high fidelity polynucleotides |
| WO2012078312A2 (en) | 2010-11-12 | 2012-06-14 | Gen9, Inc. | Methods and devices for nucleic acids synthesis |
| EP2637780B1 (de) | 2010-11-12 | 2022-02-09 | Gen9, Inc. | Proteinanordnungen und verfahren zu ihrer herstellung und verwendung |
| IL302248A (en) | 2011-08-26 | 2023-06-01 | Gen9 Inc | Compositions and methods for high fidelity assembly of nucleic acids |
| US9150853B2 (en) | 2012-03-21 | 2015-10-06 | Gen9, Inc. | Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis |
| AU2013251701A1 (en) | 2012-04-24 | 2014-10-30 | Gen9, Inc. | Methods for sorting nucleic acids and multiplexed preparative in vitro cloning |
| EP2864531B2 (de) | 2012-06-25 | 2022-08-03 | Gen9, Inc. | Verfahren zur nukleinsäurezusammenfügung und -sequenzierung mit hohem durchsatz |
| WO2014058890A1 (en) * | 2012-10-08 | 2014-04-17 | Spiral Genetics Inc. | Methods and systems for identifying, from read symbol sequences, variations with respect to a reference symbol sequence |
Family Cites Families (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5376526A (en) * | 1992-05-06 | 1994-12-27 | The Board Of Trustees Of The Leland Stanford Junior University | Genomic mismatch scanning |
| US6120992A (en) | 1993-11-04 | 2000-09-19 | Valigene Corporation | Use of immobilized mismatch binding protein for detection of mutations and polymorphisms, and allele identification in a diseased human |
| US6027877A (en) * | 1993-11-04 | 2000-02-22 | Gene Check, Inc. | Use of immobilized mismatch binding protein for detection of mutations and polymorphisms, purification of amplified DNA samples and allele identification |
| EP1041160A4 (de) | 1997-07-31 | 2003-05-28 | Rikagaku Kenkyusho | Verfahren zur detektion einer mutation innerhalb einer basensequenz |
| US6670127B2 (en) | 1997-09-16 | 2003-12-30 | Egea Biosciences, Inc. | Method for assembly of a polynucleotide encoding a target polypeptide |
| US6297010B1 (en) * | 1998-01-30 | 2001-10-02 | Genzyme Corporation | Method for detecting and identifying mutations |
| GB9808145D0 (en) * | 1998-04-17 | 1998-06-17 | Zeneca Ltd | Assay |
| US6287825B1 (en) * | 1998-09-18 | 2001-09-11 | Molecular Staging Inc. | Methods for reducing the complexity of DNA sequences |
| WO2000049142A1 (de) | 1999-02-19 | 2000-08-24 | Febit Ferrarius Biotechnology Gmbh | Verfahren zur herstellung von polymeren |
| WO2000053617A1 (en) | 1999-03-08 | 2000-09-14 | Protogene Laboratories, Inc. | Methods and compositions for economically synthesizing and assembling long dna sequences |
| WO2002095073A1 (en) * | 2001-05-18 | 2002-11-28 | Wisconsin Alumni Research Foundation | Method for the synthesis of dna sequences |
| US6673552B2 (en) * | 2002-01-14 | 2004-01-06 | Diversa Corporation | Methods for purifying annealed double-stranded oligonucleotides lacking base pair mismatches or nucleotide gaps |
| ATE428805T1 (de) * | 2002-02-28 | 2009-05-15 | Wisconsin Alumni Res Found | Verfahren zur fehlerreduzierung in nukleinsäurepopulationen |
| US7563600B2 (en) | 2002-09-12 | 2009-07-21 | Combimatrix Corporation | Microarray synthesis and assembly of gene-length polynucleotides |
-
2003
- 2003-02-28 AT AT03711383T patent/ATE428805T1/de not_active IP Right Cessation
- 2003-02-28 US US10/376,720 patent/US7303872B2/en not_active Expired - Fee Related
- 2003-02-28 AU AU2003213696A patent/AU2003213696B2/en not_active Ceased
- 2003-02-28 CA CA2477519A patent/CA2477519C/en not_active Expired - Fee Related
- 2003-02-28 WO PCT/US2003/006500 patent/WO2003072832A1/en not_active Ceased
- 2003-02-28 KR KR1020047013498A patent/KR101026816B1/ko not_active Expired - Fee Related
- 2003-02-28 DE DE60327183T patent/DE60327183D1/de not_active Expired - Lifetime
- 2003-02-28 IL IL16378803A patent/IL163788A0/xx unknown
- 2003-02-28 EP EP03711383A patent/EP1483409B1/de not_active Expired - Lifetime
- 2003-02-28 JP JP2003571511A patent/JP4455061B2/ja not_active Expired - Fee Related
- 2003-02-28 MX MXPA04008373A patent/MXPA04008373A/es active IP Right Grant
-
2004
- 2004-08-29 IL IL163788A patent/IL163788A/en not_active IP Right Cessation
- 2004-08-30 IS IS7430A patent/IS7430A/is unknown
-
2007
- 2007-10-23 US US11/877,130 patent/US20090188793A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| WO2003072832A1 (en) | 2003-09-04 |
| KR20040088562A (ko) | 2004-10-16 |
| IL163788A (en) | 2010-04-29 |
| EP1483409B1 (de) | 2009-04-15 |
| EP1483409A4 (de) | 2006-03-08 |
| AU2003213696A1 (en) | 2003-09-09 |
| KR101026816B1 (ko) | 2011-04-04 |
| JP4455061B2 (ja) | 2010-04-21 |
| CA2477519C (en) | 2013-01-22 |
| US7303872B2 (en) | 2007-12-04 |
| US20040132029A1 (en) | 2004-07-08 |
| US20090188793A1 (en) | 2009-07-30 |
| EP1483409A1 (de) | 2004-12-08 |
| IS7430A (is) | 2004-08-30 |
| AU2003213696B2 (en) | 2008-01-24 |
| DE60327183D1 (de) | 2009-05-28 |
| JP2005518799A (ja) | 2005-06-30 |
| MXPA04008373A (es) | 2004-11-26 |
| CA2477519A1 (en) | 2003-09-04 |
| IL163788A0 (en) | 2005-12-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| ATE428805T1 (de) | Verfahren zur fehlerreduzierung in nukleinsäurepopulationen | |
| ATE496998T1 (de) | Verfahren zur fehlerverringerung in nukleinsäurepopulationen | |
| Krjutškov et al. | Development of a single tube 640-plex genotyping method for detection of nucleic acid variations on microarrays | |
| JP2023017863A (ja) | 制御された化学量論を有するポリヌクレオチドライブラリおよびその合成 | |
| JP2024124412A (ja) | 核酸ハイブリダイゼーションのためのポリヌクレオチド、試薬、および方法 | |
| FI3810774T3 (fi) | Menetelmiä suuritehoisten yksittäissolutranskriptomikirjastojen valmistamiseksi | |
| AU2014377372B2 (en) | Polynucleotide modification on solid support | |
| RU2565550C2 (ru) | Прямой захват, амплификация и секвенирование днк-мишени с использованием иммобилизированных праймеров | |
| US9238834B2 (en) | Efficient shotgun sequencing methods | |
| EP2611939B1 (de) | Methoden zur amplifizierung von nukleinsäuren | |
| CN105647907B (zh) | 一种用于靶向杂交捕获的修饰性dna杂交探针的制备方法 | |
| CN117106868A (zh) | 一种扩增靶核酸的试剂盒和方法 | |
| US20070250809A1 (en) | Computer Software For Visualizing Genotyping Data | |
| EP1645640B1 (de) | Verfahren zur Detektion von chromosomalen Translokationen | |
| WO2015014962A1 (en) | Sequence capture method using specialized capture probes (heatseq) | |
| US20110092380A1 (en) | Improved molecular-biological processing equipment | |
| CA3099909A1 (en) | Chemical compositions and methods of using same | |
| US10557135B2 (en) | Sequence tags | |
| CN105431555A (zh) | 用于亚硫酸氢盐转化的序列捕获的组合物和方法 | |
| Mir et al. | Sequencing by cyclic ligation and cleavage (CycLiC) directly on a microarray captured template | |
| CN102782152A (zh) | Rna分析方法 | |
| US7629164B2 (en) | Methods for genotyping polymorphisms in humans | |
| DE602004005439D1 (de) | Ligations-basierende synthese von oligonucleotiden mit blockstrukturen | |
| CN109750092A (zh) | 一种靶向富集高gc含量目标dna的方法和试剂盒 | |
| Sobrino et al. | SNP genotyping with single base extension-tag microarrays |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |