DE60327183D1 - Verfahren zur fehlerreduzierung in nukleinsäurepopulationen - Google Patents

Verfahren zur fehlerreduzierung in nukleinsäurepopulationen

Info

Publication number
DE60327183D1
DE60327183D1 DE60327183T DE60327183T DE60327183D1 DE 60327183 D1 DE60327183 D1 DE 60327183D1 DE 60327183 T DE60327183 T DE 60327183T DE 60327183 T DE60327183 T DE 60327183T DE 60327183 D1 DE60327183 D1 DE 60327183D1
Authority
DE
Germany
Prior art keywords
dna
segments
microarray
substrate
pulpations
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60327183T
Other languages
English (en)
Inventor
Michael R Sussman
Francesco Cerrina
Peter J Belshaw
James H Kaysen
Kathryn Richmond
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Wisconsin Alumni Research Foundation
Original Assignee
Wisconsin Alumni Research Foundation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wisconsin Alumni Research Foundation filed Critical Wisconsin Alumni Research Foundation
Application granted granted Critical
Publication of DE60327183D1 publication Critical patent/DE60327183D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Paper (AREA)
  • Saccharide Compounds (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
DE60327183T 2002-02-28 2003-02-28 Verfahren zur fehlerreduzierung in nukleinsäurepopulationen Expired - Lifetime DE60327183D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US36056302P 2002-02-28 2002-02-28
PCT/US2003/006500 WO2003072832A1 (en) 2002-02-28 2003-02-28 Method of error reduction in nucleic acid populations

Publications (1)

Publication Number Publication Date
DE60327183D1 true DE60327183D1 (de) 2009-05-28

Family

ID=27766234

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60327183T Expired - Lifetime DE60327183D1 (de) 2002-02-28 2003-02-28 Verfahren zur fehlerreduzierung in nukleinsäurepopulationen

Country Status (12)

Country Link
US (2) US7303872B2 (de)
EP (1) EP1483409B1 (de)
JP (1) JP4455061B2 (de)
KR (1) KR101026816B1 (de)
AT (1) ATE428805T1 (de)
AU (1) AU2003213696B2 (de)
CA (1) CA2477519C (de)
DE (1) DE60327183D1 (de)
IL (2) IL163788A0 (de)
IS (1) IS7430A (de)
MX (1) MXPA04008373A (de)
WO (1) WO2003072832A1 (de)

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE60227943D1 (de) 2001-05-18 2008-09-11 Wisconsin Alumni Res Found Verfahren zur synthese von dna-sequenzen die photolabile linker verwenden
KR101026816B1 (ko) * 2002-02-28 2011-04-04 위스콘신 얼럼나이 리서어치 화운데이션 핵산 집단내 오류 저감 방법
US7563600B2 (en) 2002-09-12 2009-07-21 Combimatrix Corporation Microarray synthesis and assembly of gene-length polynucleotides
US8133670B2 (en) 2003-06-13 2012-03-13 Cold Spring Harbor Laboratory Method for making populations of defined nucleic acid molecules
US8105780B2 (en) * 2003-09-22 2012-01-31 Agency For Science, Technology And Research Device and method of detecting mutations and polymorphisms in DNA
WO2005089110A2 (en) * 2004-02-27 2005-09-29 President And Fellows Of Harvard College Polynucleotide synthesis
EP1574570A1 (de) * 2004-03-12 2005-09-14 Universität Regensburg Verfahren zur Verringerung der Anzahl von fehlgepaarten Nukleotiden in doppelsträngigen Polynukleotiden
US20100016178A1 (en) * 2004-06-09 2010-01-21 Sussman Michael R Methods for rapid production of target double-stranded dna sequences
CA2578564C (en) 2004-08-27 2013-05-28 Wisconsin Alumni Research Foundation Method of error reduction in nucleic acid populations
US20070269870A1 (en) * 2004-10-18 2007-11-22 George Church Methods for assembly of high fidelity synthetic polynucleotides
US20070122817A1 (en) * 2005-02-28 2007-05-31 George Church Methods for assembly of high fidelity synthetic polynucleotides
WO2007016502A2 (en) 2005-08-02 2007-02-08 Wisconsin Alumni Research Foundation Synthesis of arrays of oligonucleotides and other chain molecules
US20070196834A1 (en) * 2005-09-09 2007-08-23 Francesco Cerrina Method and system for the generation of large double stranded DNA fragments
US20070231805A1 (en) * 2006-03-31 2007-10-04 Baynes Brian M Nucleic acid assembly optimization using clamped mismatch binding proteins
US20090087840A1 (en) * 2006-05-19 2009-04-02 Codon Devices, Inc. Combined extension and ligation for nucleic acid assembly
US8053191B2 (en) 2006-08-31 2011-11-08 Westend Asset Clearinghouse Company, Llc Iterative nucleic acid assembly using activation of vector-encoded traits
US10207240B2 (en) 2009-11-03 2019-02-19 Gen9, Inc. Methods and microfluidic devices for the manipulation of droplets in high fidelity polynucleotide assembly
EP2504449B1 (de) 2009-11-25 2016-03-23 Gen9, Inc. Verfahren und vorrichtung für chipbasierte dna-fehlerreduktion
US9216414B2 (en) 2009-11-25 2015-12-22 Gen9, Inc. Microfluidic devices and methods for gene synthesis
WO2011085075A2 (en) 2010-01-07 2011-07-14 Gen9, Inc. Assembly of high fidelity polynucleotides
CA3132011A1 (en) 2010-11-12 2012-06-14 Gen9, Inc. Methods and devices for nucleic acids synthesis
US10457935B2 (en) 2010-11-12 2019-10-29 Gen9, Inc. Protein arrays and methods of using and making the same
WO2013032850A2 (en) 2011-08-26 2013-03-07 Gen9, Inc. Compositions and methods for high fidelity assembly of nucleic acids
US9150853B2 (en) 2012-03-21 2015-10-06 Gen9, Inc. Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis
AU2013251701A1 (en) 2012-04-24 2014-10-30 Gen9, Inc. Methods for sorting nucleic acids and multiplexed preparative in vitro cloning
EP3483311A1 (de) 2012-06-25 2019-05-15 Gen9, Inc. Verfahren zur nukleinsäurezusammenfügung und -sequenzierung mit hohem durchsatz
CA2885058A1 (en) * 2012-10-08 2014-04-17 Spiral Genetics Inc. Methods and systems for identifying, from read symbol sequences, variations with respect to a reference symbol sequence

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5376526A (en) * 1992-05-06 1994-12-27 The Board Of Trustees Of The Leland Stanford Junior University Genomic mismatch scanning
US6120992A (en) * 1993-11-04 2000-09-19 Valigene Corporation Use of immobilized mismatch binding protein for detection of mutations and polymorphisms, and allele identification in a diseased human
US6027877A (en) * 1993-11-04 2000-02-22 Gene Check, Inc. Use of immobilized mismatch binding protein for detection of mutations and polymorphisms, purification of amplified DNA samples and allele identification
CA2267642A1 (en) * 1997-07-31 1999-02-11 Yoshihide Hayashizaki Methods for detecting mutation in base sequence
US6670127B2 (en) * 1997-09-16 2003-12-30 Egea Biosciences, Inc. Method for assembly of a polynucleotide encoding a target polypeptide
US6297010B1 (en) * 1998-01-30 2001-10-02 Genzyme Corporation Method for detecting and identifying mutations
GB9808145D0 (en) * 1998-04-17 1998-06-17 Zeneca Ltd Assay
US6287825B1 (en) * 1998-09-18 2001-09-11 Molecular Staging Inc. Methods for reducing the complexity of DNA sequences
AU767606B2 (en) * 1999-02-19 2003-11-20 Synthetic Genomics, Inc. Method for producing polymers
ATE296310T1 (de) * 1999-03-08 2005-06-15 Metrigen Inc Syntheseverfahren zum ökonomischen aufbau langer dna-sequenzen und zusammensetzungen hierfür
DE60227943D1 (de) * 2001-05-18 2008-09-11 Wisconsin Alumni Res Found Verfahren zur synthese von dna-sequenzen die photolabile linker verwenden
US6673552B2 (en) * 2002-01-14 2004-01-06 Diversa Corporation Methods for purifying annealed double-stranded oligonucleotides lacking base pair mismatches or nucleotide gaps
KR101026816B1 (ko) * 2002-02-28 2011-04-04 위스콘신 얼럼나이 리서어치 화운데이션 핵산 집단내 오류 저감 방법
US7563600B2 (en) * 2002-09-12 2009-07-21 Combimatrix Corporation Microarray synthesis and assembly of gene-length polynucleotides

Also Published As

Publication number Publication date
AU2003213696B2 (en) 2008-01-24
CA2477519C (en) 2013-01-22
AU2003213696A1 (en) 2003-09-09
EP1483409A1 (de) 2004-12-08
US20090188793A1 (en) 2009-07-30
WO2003072832A1 (en) 2003-09-04
EP1483409B1 (de) 2009-04-15
JP2005518799A (ja) 2005-06-30
US7303872B2 (en) 2007-12-04
JP4455061B2 (ja) 2010-04-21
KR20040088562A (ko) 2004-10-16
US20040132029A1 (en) 2004-07-08
IL163788A (en) 2010-04-29
EP1483409A4 (de) 2006-03-08
ATE428805T1 (de) 2009-05-15
IL163788A0 (en) 2005-12-18
KR101026816B1 (ko) 2011-04-04
IS7430A (is) 2004-08-30
MXPA04008373A (es) 2004-11-26
CA2477519A1 (en) 2003-09-04

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