DE602004005439D1 - Ligations-basierende synthese von oligonucleotiden mit blockstrukturen - Google Patents

Ligations-basierende synthese von oligonucleotiden mit blockstrukturen

Info

Publication number
DE602004005439D1
DE602004005439D1 DE602004005439T DE602004005439T DE602004005439D1 DE 602004005439 D1 DE602004005439 D1 DE 602004005439D1 DE 602004005439 T DE602004005439 T DE 602004005439T DE 602004005439 T DE602004005439 T DE 602004005439T DE 602004005439 D1 DE602004005439 D1 DE 602004005439D1
Authority
DE
Germany
Prior art keywords
oligo
polynucleotides
polynucleotide
terminus
nucleic acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE602004005439T
Other languages
English (en)
Inventor
Aleksey Soldatov
Tatiana Borodina
Hans Lehrach
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Max Planck Gesellschaft zur Foerderung der Wissenschaften eV
Original Assignee
Max Planck Gesellschaft zur Foerderung der Wissenschaften eV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Max Planck Gesellschaft zur Foerderung der Wissenschaften eV filed Critical Max Planck Gesellschaft zur Foerderung der Wissenschaften eV
Publication of DE602004005439D1 publication Critical patent/DE602004005439D1/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
DE602004005439T 2003-04-15 2004-04-14 Ligations-basierende synthese von oligonucleotiden mit blockstrukturen Expired - Fee Related DE602004005439D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP03008653 2003-04-15
PCT/EP2004/003921 WO2004092375A2 (en) 2003-04-15 2004-04-14 Ligation-based synthesis of oligonucleotides with block structure

Publications (1)

Publication Number Publication Date
DE602004005439D1 true DE602004005439D1 (de) 2007-05-03

Family

ID=33185845

Family Applications (1)

Application Number Title Priority Date Filing Date
DE602004005439T Expired - Fee Related DE602004005439D1 (de) 2003-04-15 2004-04-14 Ligations-basierende synthese von oligonucleotiden mit blockstrukturen

Country Status (5)

Country Link
US (1) US20090035823A1 (de)
EP (1) EP1616008B1 (de)
AT (1) ATE357515T1 (de)
DE (1) DE602004005439D1 (de)
WO (1) WO2004092375A2 (de)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2003279089A1 (en) * 2002-09-30 2004-04-19 Parallele Bioscience, Inc. Polynucleotide synthesis and labeling by kinetic sampling ligation
US9222936B2 (en) 2007-04-18 2015-12-29 Solulink, Inc. Methods and/or use of oligonucleotide conjugates for suppressing background due to cross-hybridization
WO2009138954A2 (en) 2008-05-14 2009-11-19 British Columbia Cancer Agency Branch Gene synthesis by convergent assembly of oligonucleotide subsets
CN102625850B (zh) * 2009-04-03 2014-11-26 蒂莫西·Z·刘 多重核酸检测方法和系统
US20130123121A1 (en) * 2010-11-22 2013-05-16 The University Of Chicago Methods and/or Use of Oligonucleotide-Bead Conjugates for Assays and Detections
CA2848304A1 (en) 2011-09-09 2013-03-14 The Board Of Trustees Of The Leland Stanford Junior University Methods for sequencing a polynucleotide
AU2014363967A1 (en) * 2013-12-09 2017-01-05 Shawn Allen Long nucleic acid sequences containing variable regions
EP2883963A1 (de) * 2013-12-16 2015-06-17 Alacris Theranostics GmbH Verfahren zur Erzeugung von Oligonukleotid-Arrays mit In-situ-Blocksyntheseansatz
US20180216253A1 (en) * 2017-01-31 2018-08-02 Counsyl, Inc. Sequencing library preparation in small well format
EP3607092B1 (de) * 2018-01-12 2022-12-21 Stem Arts Projects, LLC Reaktionskonditionierungszusammensetzung zur zirkularisierung von oligonukleotidsonden

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE58908694D1 (de) * 1988-05-24 1995-01-12 Biotechnolog Forschung Gmbh Oligonucleotidbank und verfahren zur dna-sequenzierung.
US5851804A (en) * 1996-05-06 1998-12-22 Apollon, Inc. Chimeric kanamycin resistance gene
US5942609A (en) * 1998-11-12 1999-08-24 The Porkin-Elmer Corporation Ligation assembly and detection of polynucleotides on solid-support
ATE431407T1 (de) * 2002-01-11 2009-05-15 Biospring Ges Fuer Biotechnolo Verfahren zur herstellung von dna
JP2005529606A (ja) * 2002-06-12 2005-10-06 データスコープ・インベストメント・コーポレイション ポリマー標識分子

Also Published As

Publication number Publication date
WO2004092375A2 (en) 2004-10-28
EP1616008A2 (de) 2006-01-18
WO2004092375A3 (en) 2005-01-06
ATE357515T1 (de) 2007-04-15
EP1616008B1 (de) 2007-03-21
US20090035823A1 (en) 2009-02-05

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Legal Events

Date Code Title Description
8339 Ceased/non-payment of the annual fee