ATE357515T1 - Ligations-basierende synthese von oligonucleotiden mit blockstrukturen - Google Patents

Ligations-basierende synthese von oligonucleotiden mit blockstrukturen

Info

Publication number
ATE357515T1
ATE357515T1 AT04727252T AT04727252T ATE357515T1 AT E357515 T1 ATE357515 T1 AT E357515T1 AT 04727252 T AT04727252 T AT 04727252T AT 04727252 T AT04727252 T AT 04727252T AT E357515 T1 ATE357515 T1 AT E357515T1
Authority
AT
Austria
Prior art keywords
oligo
polynucleotides
polynucleotide
terminus
nucleic acid
Prior art date
Application number
AT04727252T
Other languages
English (en)
Inventor
Aleksey Soldatov
Tatiana Borodina
Hans Lehrach
Original Assignee
Max Planck Gesellschaft
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Max Planck Gesellschaft filed Critical Max Planck Gesellschaft
Application granted granted Critical
Publication of ATE357515T1 publication Critical patent/ATE357515T1/de

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/26Preparation of nitrogen-containing carbohydrates
    • C12P19/28N-glycosides
    • C12P19/30Nucleotides
    • C12P19/34Polynucleotides, e.g. nucleic acids, oligoribonucleotides

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
AT04727252T 2003-04-15 2004-04-14 Ligations-basierende synthese von oligonucleotiden mit blockstrukturen ATE357515T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP03008653 2003-04-15

Publications (1)

Publication Number Publication Date
ATE357515T1 true ATE357515T1 (de) 2007-04-15

Family

ID=33185845

Family Applications (1)

Application Number Title Priority Date Filing Date
AT04727252T ATE357515T1 (de) 2003-04-15 2004-04-14 Ligations-basierende synthese von oligonucleotiden mit blockstrukturen

Country Status (5)

Country Link
US (1) US20090035823A1 (de)
EP (1) EP1616008B1 (de)
AT (1) ATE357515T1 (de)
DE (1) DE602004005439D1 (de)
WO (1) WO2004092375A2 (de)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004029223A2 (en) * 2002-09-30 2004-04-08 Parallele Bioscience, Inc. Polynucleotide synthesis and labeling by kinetic sampling ligation
US20120258880A1 (en) * 2010-11-22 2012-10-11 The University Of Chicago Methods and/or Use of Oligonucleotide Conjugates for Assays and Flow Cytometry Detections
US9222936B2 (en) 2007-04-18 2015-12-29 Solulink, Inc. Methods and/or use of oligonucleotide conjugates for suppressing background due to cross-hybridization
CA2723242A1 (en) 2008-05-14 2009-11-19 British Columbia Cancer Agency Branch Gene synthesis by convergent assembly of oligonucleotide subsets
CN104404134B (zh) * 2009-04-03 2017-05-10 莱弗斯基因股份有限公司 多重核酸检测方法和系统
WO2013036929A1 (en) 2011-09-09 2013-03-14 The Board Of Trustees Of The Leland Stanford Junior Methods for obtaining a sequence
US20150159152A1 (en) * 2013-12-09 2015-06-11 Integrated Dna Technologies, Inc. Long nucleic acid sequences containing variable regions
EP2883963A1 (de) * 2013-12-16 2015-06-17 Alacris Theranostics GmbH Verfahren zur Erzeugung von Oligonukleotid-Arrays mit In-situ-Blocksyntheseansatz
US20180216253A1 (en) * 2017-01-31 2018-08-02 Counsyl, Inc. Sequencing library preparation in small well format
US20200199661A1 (en) * 2018-01-12 2020-06-25 Stem Arts Projects, Llc Reaction condition composition for circularizing oligonucleotide probes

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5114839A (en) * 1988-05-24 1992-05-19 Gesellschaft Fur Biotechnologische Forsching Mbh Process for dna sequencing using oligonucleotide bank
US5851804A (en) * 1996-05-06 1998-12-22 Apollon, Inc. Chimeric kanamycin resistance gene
US5942609A (en) * 1998-11-12 1999-08-24 The Porkin-Elmer Corporation Ligation assembly and detection of polynucleotides on solid-support
ATE431407T1 (de) * 2002-01-11 2009-05-15 Biospring Ges Fuer Biotechnolo Verfahren zur herstellung von dna
JP2005529606A (ja) * 2002-06-12 2005-10-06 データスコープ・インベストメント・コーポレイション ポリマー標識分子

Also Published As

Publication number Publication date
US20090035823A1 (en) 2009-02-05
EP1616008B1 (de) 2007-03-21
WO2004092375A3 (en) 2005-01-06
EP1616008A2 (de) 2006-01-18
WO2004092375A2 (en) 2004-10-28
DE602004005439D1 (de) 2007-05-03

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