ATE462005T1 - Iterierte verzweigende reaktionswege über nukleinsäure-vermittelte chemie - Google Patents

Iterierte verzweigende reaktionswege über nukleinsäure-vermittelte chemie

Info

Publication number
ATE462005T1
ATE462005T1 AT06785057T AT06785057T ATE462005T1 AT E462005 T1 ATE462005 T1 AT E462005T1 AT 06785057 T AT06785057 T AT 06785057T AT 06785057 T AT06785057 T AT 06785057T AT E462005 T1 ATE462005 T1 AT E462005T1
Authority
AT
Austria
Prior art keywords
nucleic acid
iterated
pathways via
via nucleic
mediated chemistry
Prior art date
Application number
AT06785057T
Other languages
English (en)
Inventor
Christopher Calderone
David Liu
Original Assignee
Harvard College
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Harvard College filed Critical Harvard College
Application granted granted Critical
Publication of ATE462005T1 publication Critical patent/ATE462005T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1068—Template (nucleic acid) mediated chemical library synthesis, e.g. chemical and enzymatical DNA-templated organic molecule synthesis, libraries prepared by non ribosomal polypeptide synthesis [NRPS], DNA/RNA-polymerase mediated polypeptide synthesis

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
AT06785057T 2005-06-17 2006-06-16 Iterierte verzweigende reaktionswege über nukleinsäure-vermittelte chemie ATE462005T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US69162805P 2005-06-17 2005-06-17
PCT/US2006/023654 WO2006138666A2 (en) 2005-06-17 2006-06-16 Iterated branching reaction pathways via nucleic acid-mediated chemistry

Publications (1)

Publication Number Publication Date
ATE462005T1 true ATE462005T1 (de) 2010-04-15

Family

ID=37571264

Family Applications (1)

Application Number Title Priority Date Filing Date
AT06785057T ATE462005T1 (de) 2005-06-17 2006-06-16 Iterierte verzweigende reaktionswege über nukleinsäure-vermittelte chemie

Country Status (5)

Country Link
US (1) US8183178B2 (de)
EP (1) EP1899465B1 (de)
AT (1) ATE462005T1 (de)
DE (1) DE602006013134D1 (de)
WO (1) WO2006138666A2 (de)

Families Citing this family (45)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL158000A0 (en) 2001-03-19 2004-03-28 Harvard College Evolving new molecular function
EP1401850A1 (de) 2001-06-20 2004-03-31 Nuevolution A/S Nukleosidderivate zur herstellung einer verbindungsbibliothek
IL163822A0 (en) 2002-03-15 2005-12-18 Nuevolution As An improved method for synthesising templated molecules
AU2003247266A1 (en) 2002-08-01 2004-02-23 Nuevolution A/S Multi-step synthesis of templated molecules
WO2004016767A2 (en) 2002-08-19 2004-02-26 The President And Fellows Of Harvard College Evolving new molecular function
PT1558744E (pt) 2002-10-30 2011-09-22 Nuevolution As Codificação enzimática
WO2004056994A2 (en) 2002-12-19 2004-07-08 Nuevolution A/S Quasirandom structure and function guided synthesis methods
EP1597395A2 (de) 2003-02-21 2005-11-23 Nuevolution A/S Methode zur herstellung einer bibliothek der zweiten generation
US8017323B2 (en) 2003-03-26 2011-09-13 President And Fellows Of Harvard College Free reactant use in nucleic acid-templated synthesis
DE602004023960D1 (de) 2003-09-18 2009-12-17 Nuevolution As Methode zur Gewinnung struktureller Informationen kodierter Moleküle und zur Selektion von Verbindungen
US7968289B2 (en) * 2005-05-03 2011-06-28 Ensemble Therapeutics Corporation Turn over probes and use thereof for nucleic acid detection
ATE462005T1 (de) 2005-06-17 2010-04-15 Harvard College Iterierte verzweigende reaktionswege über nukleinsäure-vermittelte chemie
US9574189B2 (en) 2005-12-01 2017-02-21 Nuevolution A/S Enzymatic encoding methods for efficient synthesis of large libraries
EP2350031A2 (de) * 2008-08-20 2011-08-03 Ensemble Therapeutics Corporation Makrocyclische verbindungen zur inhibierung des tumornekrosefaktors alpha
ES2713873T3 (es) 2010-04-16 2019-05-24 Nuevolution As Complejos bifuncionales y métodos para hacer y utilizar tales complejos
EP2734621B1 (de) 2011-07-22 2019-09-04 President and Fellows of Harvard College Beurteilung und verbesserung einer nukleasespaltungsspezifität
US9163284B2 (en) 2013-08-09 2015-10-20 President And Fellows Of Harvard College Methods for identifying a target site of a Cas9 nuclease
US9340800B2 (en) 2013-09-06 2016-05-17 President And Fellows Of Harvard College Extended DNA-sensing GRNAS
US9322037B2 (en) 2013-09-06 2016-04-26 President And Fellows Of Harvard College Cas9-FokI fusion proteins and uses thereof
CA2956224A1 (en) 2014-07-30 2016-02-11 President And Fellows Of Harvard College Cas9 proteins including ligand-dependent inteins
EP3218479A1 (de) 2014-11-11 2017-09-20 Nanocore ApS Verfahren zur identifizierung von molekülen mit gewünschten eigenschaften
EP4434589A3 (de) 2015-10-23 2025-05-14 President and Fellows of Harvard College Entwickelte cas9-proteine für geneditierung
JP7283727B2 (ja) 2016-06-16 2023-05-30 ヘイスタック サイエンシィズ コーポレーション コードプローブ分子のコンビナトリアル合成が指令されかつ記録されたオリゴヌクレオチド
JP7231935B2 (ja) 2016-08-03 2023-03-08 プレジデント アンド フェローズ オブ ハーバード カレッジ アデノシン核酸塩基編集因子およびそれらの使用
US11661590B2 (en) 2016-08-09 2023-05-30 President And Fellows Of Harvard College Programmable CAS9-recombinase fusion proteins and uses thereof
WO2018039438A1 (en) 2016-08-24 2018-03-01 President And Fellows Of Harvard College Incorporation of unnatural amino acids into proteins using base editing
WO2018119359A1 (en) 2016-12-23 2018-06-28 President And Fellows Of Harvard College Editing of ccr5 receptor gene to protect against hiv infection
CN110662556A (zh) 2017-03-09 2020-01-07 哈佛大学的校长及成员们 癌症疫苗
US11898179B2 (en) 2017-03-09 2024-02-13 President And Fellows Of Harvard College Suppression of pain by gene editing
JP2020510439A (ja) 2017-03-10 2020-04-09 プレジデント アンド フェローズ オブ ハーバード カレッジ シトシンからグアニンへの塩基編集因子
EP3619340A4 (de) 2017-05-02 2021-01-20 Haystack Sciences Corporation Moleküle zur verifizierung von oligonukleotidgerichteter kombinatorischer synthese und verfahren zur herstellung und verwendung davon
US11560566B2 (en) 2017-05-12 2023-01-24 President And Fellows Of Harvard College Aptazyme-embedded guide RNAs for use with CRISPR-Cas9 in genome editing and transcriptional activation
US11732274B2 (en) 2017-07-28 2023-08-22 President And Fellows Of Harvard College Methods and compositions for evolving base editors using phage-assisted continuous evolution (PACE)
WO2019139645A2 (en) 2017-08-30 2019-07-18 President And Fellows Of Harvard College High efficiency base editors comprising gam
KR20200121782A (ko) 2017-10-16 2020-10-26 더 브로드 인스티튜트, 인코퍼레이티드 아데노신 염기 편집제의 용도
US12406749B2 (en) 2017-12-15 2025-09-02 The Broad Institute, Inc. Systems and methods for predicting repair outcomes in genetic engineering
US12157760B2 (en) 2018-05-23 2024-12-03 The Broad Institute, Inc. Base editors and uses thereof
US12522807B2 (en) 2018-07-09 2026-01-13 The Broad Institute, Inc. RNA programmable epigenetic RNA modifiers and uses thereof
WO2020092453A1 (en) 2018-10-29 2020-05-07 The Broad Institute, Inc. Nucleobase editors comprising geocas9 and uses thereof
US12203130B2 (en) 2018-12-20 2025-01-21 Krylov Sergey N Binder selection using capillary electrophoresis
WO2020154500A1 (en) 2019-01-23 2020-07-30 The Broad Institute, Inc. Supernegatively charged proteins and uses thereof
MX2021011426A (es) 2019-03-19 2022-03-11 Broad Inst Inc Metodos y composiciones para editar secuencias de nucleótidos.
WO2020214842A1 (en) 2019-04-17 2020-10-22 The Broad Institute, Inc. Adenine base editors with reduced off-target effects
US12435330B2 (en) 2019-10-10 2025-10-07 The Broad Institute, Inc. Methods and compositions for prime editing RNA
WO2021226558A1 (en) 2020-05-08 2021-11-11 The Broad Institute, Inc. Methods and compositions for simultaneous editing of both strands of a target double-stranded nucleotide sequence

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2346989A1 (en) * 1998-10-19 2000-04-27 The Board Of Trustees Of The Leland Stanford Junior University Dna-templated combinatorial library chemistry
IL158000A0 (en) * 2001-03-19 2004-03-28 Harvard College Evolving new molecular function
WO2004016767A2 (en) * 2002-08-19 2004-02-26 The President And Fellows Of Harvard College Evolving new molecular function
AU2003260291A1 (en) 2002-09-12 2004-04-30 Nuevolution A/S Proximity-aided synthesis of templated molecules
PT1558744E (pt) 2002-10-30 2011-09-22 Nuevolution As Codificação enzimática
US8017323B2 (en) * 2003-03-26 2011-09-13 President And Fellows Of Harvard College Free reactant use in nucleic acid-templated synthesis
WO2006130669A2 (en) * 2005-05-31 2006-12-07 Ensemble Discovery Corporation Anchor-assisted fragment selection and directed assembly
EP1888746B1 (de) * 2005-06-07 2010-03-17 President And Fellows Of Harvard College Geordnete mehrschrittsynthese mittels nukleinsäurevermittelter chemie
US20090203530A1 (en) * 2005-06-07 2009-08-13 Liu David R Polymer evolution via templated synthesis
ATE462005T1 (de) 2005-06-17 2010-04-15 Harvard College Iterierte verzweigende reaktionswege über nukleinsäure-vermittelte chemie
US20090035824A1 (en) * 2005-06-17 2009-02-05 Liu David R Nucleic acid-templated chemistry in organic solvents
WO2009105657A1 (en) * 2008-02-22 2009-08-27 Ensemble Discovery Corporation Compositions and methods for catalyzing dna-programmed chemistry
EP2350031A2 (de) * 2008-08-20 2011-08-03 Ensemble Therapeutics Corporation Makrocyclische verbindungen zur inhibierung des tumornekrosefaktors alpha

Also Published As

Publication number Publication date
EP1899465B1 (de) 2010-03-24
EP1899465A2 (de) 2008-03-19
WO2006138666A2 (en) 2006-12-28
WO2006138666A3 (en) 2007-07-19
US20080318807A1 (en) 2008-12-25
US8183178B2 (en) 2012-05-22
DE602006013134D1 (de) 2010-05-06

Similar Documents

Publication Publication Date Title
ATE462005T1 (de) Iterierte verzweigende reaktionswege über nukleinsäure-vermittelte chemie
EP4239080A3 (de) Verfahren zur analyse eines rna-moleküls
WO2007123744A3 (en) Systems and devices for sequence by synthesis analysis
DK2385141T3 (da) Flerkomponentnukleinsyreenzymer og fremgangsmåder til anvendelse heraf
WO2010026488A3 (en) Methods and kits for nucleic acid sequencing
DK2341140T3 (da) Fremgangsmåde til enzymatisk kodning ved effektiv syntese af store biblioteker
WO2009092564A3 (en) Integrated instrument performing synthesis and amplification
EP4624589A3 (de) Verfahren und systeme zur verarbeitung von polynukleotiden
ATE550441T1 (de) Verfahren, zusammensetzungen und kits zur isothermischen amplifikation von nukleinsäuren
WO2005089508A3 (en) Dna sequence detection by limited primer extension
EP2044191A4 (de) Systeme und verfahren zur überwachung des amplifikations- und dissoziationsverhaltens von dna-molekülen
WO2009076485A3 (en) Sequencing of nucleic acids
ATE483804T1 (de) Verfahren zur zuordnung von nukleinsäuren und zur identifikation fein strukturierter variationen in nukleinsäuren sowie hilfsmittel dafür
WO2007093050A8 (en) Gene expression assays conducted by elemental analysis
SI2089343T1 (sl) Klik kemija za proizvodnjo poročevalskih molekul
WO2010129019A3 (en) Real-time sequencing methods and systems
MX2013011737A (es) Metodo y producto para le deteccion localizada o espacial de acido nucleico en una muestra de tejido.
WO2006138560A3 (en) Nucleic acid-templated chemistry in organic solvents
WO2012104399A3 (en) Device and method for the generation of molecular microarrays
WO2007106802A8 (en) Method for linear amplification of bisulfite converted dna
WO2009002034A3 (en) Real-time pcr monitoring apparatus
WO2012011660A3 (ko) 시료 내장 마이크로 챔버 플레이트 및 분석용 마이크로 챔버 프레이트의 제조 방법, 분석용 마이크로 챔버 플레이트 및 시료 내장 마이크로 챔버 플레이트 제조 장치 셋
BRPI0513308A (pt) sistemas e métodos de análise de polìmeros de ácido nucléico e componentes relacionados
ATE461279T1 (de) Geordnete mehrschrittsynthese mittels nukleinsäurevermittelter chemie
ATE551433T1 (de) Verfahren und produkte zum erfassen von nukleinsäurezielen

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties