CN106715714B - 一种用于核酸随机片段化的引物及核酸随机片段化方法 - Google Patents
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Abstract
Description
测序读长数目(M) | 4.5 |
比对率 | 99.70% |
唯一比对的读长数目(M) | 4.5 |
基因组覆盖度 | 99.99% |
4X深度下基因组覆盖度 | 99.89% |
10X深度下基因组覆盖度 | 98.53% |
20X深度下基因组覆盖度 | 96.25% |
30X深度下基因组覆盖度 | 85.20% |
40X深度下基因组覆盖度 | 73.55% |
50X深度下基因组覆盖度 | 55.60% |
Claims (6)
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PCT/CN2014/088809 WO2016058173A1 (zh) | 2014-10-17 | 2014-10-17 | 一种用于核酸随机片段化的引物及核酸随机片段化方法 |
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CN106715714A CN106715714A (zh) | 2017-05-24 |
CN106715714B true CN106715714B (zh) | 2021-11-09 |
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US (1) | US10563196B2 (zh) |
EP (1) | EP3208344B1 (zh) |
CN (1) | CN106715714B (zh) |
WO (1) | WO2016058173A1 (zh) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
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WO2016058121A1 (zh) * | 2014-10-13 | 2016-04-21 | 深圳华大基因科技有限公司 | 一种核酸片段化方法和序列组合 |
CN113557300A (zh) * | 2019-03-22 | 2021-10-26 | 深圳华大智造科技股份有限公司 | 核酸序列、rna目标区域测序文库的构建方法及应用 |
Family Cites Families (17)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA2245888C (en) * | 1996-02-14 | 2008-12-23 | Jaap Goudsmit | Isolation and amplification of nucleic acid materials |
WO2002059353A2 (en) * | 2001-01-26 | 2002-08-01 | Bio S & T | Two-step amplification using a program primer followed by specific primers |
WO2004081225A2 (en) * | 2003-03-07 | 2004-09-23 | Rubicon Genomics, Inc. | Amplification and analysis of whole genome and whole transcriptome libraries generated by a dna polymerization process |
US20040209299A1 (en) | 2003-03-07 | 2004-10-21 | Rubicon Genomics, Inc. | In vitro DNA immortalization and whole genome amplification using libraries generated from randomly fragmented DNA |
CN1824786A (zh) * | 2005-12-23 | 2006-08-30 | 上海生物芯片有限公司 | 基于连接的选择性扩增方法 |
US8518640B2 (en) * | 2007-10-29 | 2013-08-27 | Complete Genomics, Inc. | Nucleic acid sequencing and process |
US9023769B2 (en) * | 2009-11-30 | 2015-05-05 | Complete Genomics, Inc. | cDNA library for nucleic acid sequencing |
WO2012166647A1 (en) * | 2011-05-27 | 2012-12-06 | Life Technologies Corporation | Methods for manipulating biomolecules |
GB201113214D0 (en) * | 2011-07-29 | 2011-09-14 | Univ East Anglia | Analysing sequencing bias |
CN102952895B (zh) * | 2011-08-23 | 2014-12-17 | 中国科学院上海生命科学研究院 | 一种利用测序技术检测未知病毒的方法 |
CN103930570B (zh) * | 2011-09-16 | 2018-01-09 | 莱克斯奥根有限公司 | 核酸转录方法 |
CN103060924B (zh) * | 2011-10-18 | 2016-04-20 | 深圳华大基因科技有限公司 | 微量核酸样本的文库制备方法及其应用 |
WO2013096802A1 (en) * | 2011-12-22 | 2013-06-27 | Ibis Biosciences, Inc. | Amplification primers and methods |
CN102703426A (zh) | 2012-05-21 | 2012-10-03 | 吴江汇杰生物科技有限公司 | 构建核酸库的方法、试剂及试剂盒 |
US9968901B2 (en) * | 2012-05-21 | 2018-05-15 | The Scripps Research Institute | Methods of sample preparation |
US20140274729A1 (en) | 2013-03-15 | 2014-09-18 | Nugen Technologies, Inc. | Methods, compositions and kits for generation of stranded rna or dna libraries |
CN103938277B (zh) * | 2014-04-18 | 2016-05-25 | 中国科学院北京基因组研究所 | 以痕量dna为基础的二代测序文库构建方法 |
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2014
- 2014-10-17 US US15/519,530 patent/US10563196B2/en active Active
- 2014-10-17 EP EP14904071.9A patent/EP3208344B1/en active Active
- 2014-10-17 WO PCT/CN2014/088809 patent/WO2016058173A1/zh active Application Filing
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EP3208344B1 (en) | 2020-04-22 |
EP3208344A1 (en) | 2017-08-23 |
EP3208344A4 (en) | 2018-05-30 |
CN106715714A (zh) | 2017-05-24 |
US10563196B2 (en) | 2020-02-18 |
WO2016058173A1 (zh) | 2016-04-21 |
US20170275616A1 (en) | 2017-09-28 |
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