CN103060924B - 微量核酸样本的文库制备方法及其应用 - Google Patents
微量核酸样本的文库制备方法及其应用 Download PDFInfo
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- CN103060924B CN103060924B CN201110316066.8A CN201110316066A CN103060924B CN 103060924 B CN103060924 B CN 103060924B CN 201110316066 A CN201110316066 A CN 201110316066A CN 103060924 B CN103060924 B CN 103060924B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
- C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
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- Crystallography & Structural Chemistry (AREA)
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
Claims (24)
Priority Applications (6)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201110316066.8A CN103060924B (zh) | 2011-10-18 | 2011-10-18 | 微量核酸样本的文库制备方法及其应用 |
EP12841309.3A EP2770090B1 (en) | 2011-10-18 | 2012-10-16 | Method for preparing nucleic acid library, its uses and kits |
PCT/CN2012/083036 WO2013056640A1 (zh) | 2011-10-18 | 2012-10-16 | 核酸文库的制备方法及其应用以及试剂盒 |
US14/352,492 US9359642B2 (en) | 2011-10-18 | 2012-10-16 | Method for preparing nucleic acid library, its uses and kits |
TW101138483A TW201321518A (zh) | 2011-10-18 | 2012-10-18 | 微量核酸樣本的庫製備方法及其應用 |
HK13110753.1A HK1183698A1 (zh) | 2011-10-18 | 2013-09-18 | 微量核酸樣本的文庫製備方法及其應用 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201110316066.8A CN103060924B (zh) | 2011-10-18 | 2011-10-18 | 微量核酸样本的文库制备方法及其应用 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN103060924A CN103060924A (zh) | 2013-04-24 |
CN103060924B true CN103060924B (zh) | 2016-04-20 |
Family
ID=48103808
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201110316066.8A Active CN103060924B (zh) | 2011-10-18 | 2011-10-18 | 微量核酸样本的文库制备方法及其应用 |
Country Status (6)
Country | Link |
---|---|
US (1) | US9359642B2 (zh) |
EP (1) | EP2770090B1 (zh) |
CN (1) | CN103060924B (zh) |
HK (1) | HK1183698A1 (zh) |
TW (1) | TW201321518A (zh) |
WO (1) | WO2013056640A1 (zh) |
Families Citing this family (33)
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CN104153004A (zh) * | 2014-08-11 | 2014-11-19 | 上海美吉生物医药科技有限公司 | 一种用于扩增子测序的建库方法 |
CN105368920A (zh) * | 2014-08-20 | 2016-03-02 | 北京百诺奇生物科技有限公司 | 一种检测结核分枝杆菌及其耐药基因突变的试剂盒及应用 |
CN105506063A (zh) * | 2014-09-22 | 2016-04-20 | 深圳华大基因科技有限公司 | 引物组合物及其用途 |
CN104263726A (zh) * | 2014-09-25 | 2015-01-07 | 天津诺禾致源生物信息科技有限公司 | 适用于扩增子测序文库构建的引物及扩增子测序文库的构建方法 |
CN105524983B (zh) * | 2014-09-30 | 2019-06-21 | 大连晶泰生物技术有限公司 | 基于高通量测序的标记和捕获多个样本的一个或多个特定基因的方法和试剂盒 |
US10344317B2 (en) * | 2014-10-13 | 2019-07-09 | Mgi Tech Co., Ltd | Method and a sequence combination for producing nucleic acid fragments |
CN106715714B (zh) * | 2014-10-17 | 2021-11-09 | 深圳华大智造科技股份有限公司 | 一种用于核酸随机片段化的引物及核酸随机片段化方法 |
CN104357918B (zh) * | 2014-11-25 | 2016-08-17 | 北京阅微基因技术有限公司 | 血浆游离dna文库的构建方法 |
CN104963000B (zh) * | 2014-12-15 | 2021-04-06 | 杭州贝瑞和康基因诊断技术有限公司 | 一种快速构建单细胞dna测序文库的方法和试剂盒 |
US20170349926A1 (en) * | 2014-12-22 | 2017-12-07 | DNAe Group Holdings LTD. | Bubble primers |
WO2017027835A1 (en) | 2015-08-12 | 2017-02-16 | Circulogene Theranostics, Llc | Method of preparing cell free nucleic acid molecules by in situ amplification |
CN105332063B (zh) * | 2015-08-13 | 2017-04-12 | 厦门飞朔生物技术有限公司 | 一种单管高通量测序文库的构建方法 |
CN105219766B (zh) * | 2015-11-10 | 2017-12-22 | 东华大学 | 一种三轮扩增的多重pcr方法 |
CN106555226B (zh) * | 2016-04-14 | 2019-07-23 | 大连晶泰生物技术有限公司 | 一种构建高通量测序文库的方法和试剂盒 |
CN106011230A (zh) * | 2016-05-10 | 2016-10-12 | 人和未来生物科技(长沙)有限公司 | 用于检测碎片化dna目标区域的引物组合物及其应用 |
CN106283201B (zh) * | 2016-09-20 | 2019-08-06 | 中国医学科学院肿瘤医院 | 基于高通量测序的tcr多样性检测和文库构建 |
CN106636063A (zh) * | 2016-09-27 | 2017-05-10 | 广州精科医学检验所有限公司 | 引物组合物、其用途、构建文库和确定核酸序列的方法 |
CN106554955B (zh) * | 2016-10-25 | 2020-07-14 | 大连晶泰生物技术有限公司 | 构建pkhd1基因突变的测序文库的方法和试剂盒及其用途 |
CN106554999B (zh) * | 2016-10-25 | 2020-03-17 | 大连晶泰生物技术有限公司 | 高通量检测新生儿糖尿病致病基因突变位点的测序文库构建方法、试剂盒及其用途 |
CN108504649B (zh) * | 2017-02-24 | 2021-03-05 | 金弗康生物科技(上海)股份有限公司 | 编码pcr二代测序建库方法、试剂盒及检测方法 |
JP7047373B2 (ja) * | 2017-12-25 | 2022-04-05 | トヨタ自動車株式会社 | 次世代シーケンサー用プライマー並びにその製造方法、次世代シーケンサー用プライマーを用いたdnaライブラリー並びにその製造方法、及びdnaライブラリーを用いたゲノムdna解析方法 |
CN108103175B (zh) * | 2018-01-02 | 2021-09-17 | 序康医疗科技(苏州)有限公司 | 一种用于检测eml4-alk、ros1和ret融合基因突变的方法 |
CN108103055B (zh) * | 2018-01-09 | 2021-05-28 | 序康医疗科技(苏州)有限公司 | 一种单细胞rna逆转录与文库构建的方法 |
BR112020005982A2 (pt) * | 2018-05-17 | 2020-12-08 | Illumina, Inc. | Sequenciamento de células únicas com alto rendimento com viés de amplificação reduzido |
CN109056077A (zh) * | 2018-09-13 | 2018-12-21 | 武汉菲沙基因信息有限公司 | 一种适用于PacBio测序平台的扩增子混样测序文库构建方法 |
CN112912514A (zh) * | 2018-10-17 | 2021-06-04 | 珀金埃尔默保健科学(澳大利亚)私人有限公司 | 核酸的条形编码 |
AU2020204908A1 (en) * | 2019-01-04 | 2021-07-29 | William Marsh Rice University | Quantitative amplicon sequencing for multiplexed copy number variation detection and allele ratio quantitation |
GB201911515D0 (en) * | 2019-08-12 | 2019-09-25 | Univ London Queen Mary | Methods for generating a population of polynucleotide molecules |
CN111041069B (zh) * | 2019-12-26 | 2021-01-19 | 人和未来生物科技(长沙)有限公司 | 一种低起始量dna样本的高通量测序文库构建方法及其应用 |
CN111139315A (zh) * | 2020-04-03 | 2020-05-12 | 杭州启棣生物技术有限公司 | 一种利用二代测序高通量检测呼吸道病毒的方法与应用 |
CN112359101B (zh) * | 2020-11-13 | 2023-10-03 | 苏州金唯智生物科技有限公司 | 一种质检寡核苷酸交叉污染的方法 |
CN112626214A (zh) * | 2020-12-29 | 2021-04-09 | 嘉兴允英医学检验有限公司 | 检测1p/19q杂合性缺失的引物组、试剂盒及方法 |
CN114015751A (zh) * | 2021-10-26 | 2022-02-08 | 江苏海伯基因科技有限公司 | 扩增基因组dna的方法、试剂盒及其获得扩增引物的方法 |
Citations (1)
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WO2009052128A1 (en) * | 2007-10-15 | 2009-04-23 | Sigma-Aldrich Co. | Degenerate oligonucleotides and their uses |
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GB0522310D0 (en) * | 2005-11-01 | 2005-12-07 | Solexa Ltd | Methods of preparing libraries of template polynucleotides |
US9914950B2 (en) * | 2012-08-23 | 2018-03-13 | Tufts University | Homopolymer mediated nucleic acid amplification |
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2011
- 2011-10-18 CN CN201110316066.8A patent/CN103060924B/zh active Active
-
2012
- 2012-10-16 WO PCT/CN2012/083036 patent/WO2013056640A1/zh active Application Filing
- 2012-10-16 EP EP12841309.3A patent/EP2770090B1/en active Active
- 2012-10-16 US US14/352,492 patent/US9359642B2/en active Active
- 2012-10-18 TW TW101138483A patent/TW201321518A/zh unknown
-
2013
- 2013-09-18 HK HK13110753.1A patent/HK1183698A1/zh unknown
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2009052128A1 (en) * | 2007-10-15 | 2009-04-23 | Sigma-Aldrich Co. | Degenerate oligonucleotides and their uses |
Non-Patent Citations (2)
Title |
---|
Whole genome amplification using a degenerate oligonucleotide primer allows hundreds of genotypes to be performed on less than one nanogram of genomic DNA;VG Cheung;《PNAS》;19961231;第93卷;14676-14679 * |
简并寡核苷酸引物PCR扩增单细胞基因组的影响因素研究;王晶;《安徽农业科学》;20081231;第36卷(第16期);6675-6677 * |
Also Published As
Publication number | Publication date |
---|---|
EP2770090A4 (en) | 2014-10-22 |
TW201321518A (zh) | 2013-06-01 |
CN103060924A (zh) | 2013-04-24 |
US9359642B2 (en) | 2016-06-07 |
US20140296084A1 (en) | 2014-10-02 |
WO2013056640A1 (zh) | 2013-04-25 |
EP2770090B1 (en) | 2018-04-18 |
EP2770090A1 (en) | 2014-08-27 |
HK1183698A1 (zh) | 2014-01-03 |
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