CN113557300A - 核酸序列、rna目标区域测序文库的构建方法及应用 - Google Patents

核酸序列、rna目标区域测序文库的构建方法及应用 Download PDF

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CN113557300A
CN113557300A CN201980091855.0A CN201980091855A CN113557300A CN 113557300 A CN113557300 A CN 113557300A CN 201980091855 A CN201980091855 A CN 201980091855A CN 113557300 A CN113557300 A CN 113557300A
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sequence
primer
sequencing
universal
rna
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杨林
夏军
张艳艳
吕硕
陈芳
梅智颖
陈大洋
蒋慧
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MGI Tech Co Ltd
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Abstract

提供了一种核酸序列以及RNA目标区域测序文库的构建方法及应用。该核酸序列包含相连的位于5’端的第一测序通用序列和位于3’端的随机引物序列，随机引物序列含有6～10个随机核苷酸。利用该序列对RNA进行随机的逆转录，得到cDNA的第一链，然后通过该核酸序列上的第一测序通用序列以及上下游特异性引物对逆转录得到的cDNA第一条链进行2步巢式PCR，得到高通量靶向测序文库。

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PCT国内申请，说明书已公开。

Claims (16)

  1. PCT国内申请，权利要求书已公开。
CN201980091855.0A 2019-03-22 2019-03-22 核酸序列、rna目标区域测序文库的构建方法及应用 Pending CN113557300A (zh)

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CN114317685A (zh) * 2022-01-05 2022-04-12 苏州贝康医疗器械有限公司 用于检测mRNA可变剪切变异的试剂盒、建库方法和测序方法
CN117343989A (zh) * 2023-12-06 2024-01-05 广州迈景基因医学科技有限公司 一种检测基因融合的靶向建库方法
WO2024119481A1 (zh) * 2022-12-09 2024-06-13 深圳华大智造科技股份有限公司 一种快速制备多重pcr测序文库的方法及其应用

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CN116042770B (zh) * 2022-11-01 2023-12-01 苏州京脉生物科技有限公司 尿液中miRNA文库制备和表达定量的方法及试剂盒
CN117887812B (zh) * 2024-03-14 2024-07-09 北京雅康博生物科技有限公司 高通量测序质控用文库及其制备方法和应用

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CN114317685A (zh) * 2022-01-05 2022-04-12 苏州贝康医疗器械有限公司 用于检测mRNA可变剪切变异的试剂盒、建库方法和测序方法
CN114317685B (zh) * 2022-01-05 2023-10-20 苏州贝康医疗器械有限公司 用于检测mRNA可变剪切变异的试剂盒、建库方法和测序方法
WO2024119481A1 (zh) * 2022-12-09 2024-06-13 深圳华大智造科技股份有限公司 一种快速制备多重pcr测序文库的方法及其应用
CN117343989A (zh) * 2023-12-06 2024-01-05 广州迈景基因医学科技有限公司 一种检测基因融合的靶向建库方法
CN117343989B (zh) * 2023-12-06 2024-05-17 广州迈景基因医学科技有限公司 一种检测基因融合的靶向建库方法

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