AR047721A1 - Un proceso para producir proteina exogena en la leche de mamiferos transgenicos y un proceso para purificar proteinas a partir de ella - Google Patents
Un proceso para producir proteina exogena en la leche de mamiferos transgenicos y un proceso para purificar proteinas a partir de ellaInfo
- Publication number
- AR047721A1 AR047721A1 ARP040103539A ARP040103539A AR047721A1 AR 047721 A1 AR047721 A1 AR 047721A1 AR P040103539 A ARP040103539 A AR P040103539A AR P040103539 A ARP040103539 A AR P040103539A AR 047721 A1 AR047721 A1 AR 047721A1
- Authority
- AR
- Argentina
- Prior art keywords
- transgenic
- milk
- bovine
- protein
- producing
- Prior art date
Links
- 230000009261 transgenic effect Effects 0.000 title abstract 14
- 238000000034 method Methods 0.000 title abstract 6
- 108090000623 proteins and genes Proteins 0.000 title abstract 6
- 235000013336 milk Nutrition 0.000 title abstract 5
- 239000008267 milk Substances 0.000 title abstract 5
- 210000004080 milk Anatomy 0.000 title abstract 5
- 102000004169 proteins and genes Human genes 0.000 title abstract 5
- 241000124008 Mammalia Species 0.000 title abstract 3
- 241000283690 Bos taurus Species 0.000 abstract 9
- 210000002257 embryonic structure Anatomy 0.000 abstract 3
- 235000018102 proteins Nutrition 0.000 abstract 3
- 102000002265 Human Growth Hormone Human genes 0.000 abstract 2
- 108010000521 Human Growth Hormone Proteins 0.000 abstract 2
- 239000000854 Human Growth Hormone Substances 0.000 abstract 2
- 206010042573 Superovulation Diseases 0.000 abstract 2
- 230000009027 insemination Effects 0.000 abstract 2
- 238000004519 manufacturing process Methods 0.000 abstract 2
- 210000000582 semen Anatomy 0.000 abstract 2
- 102000011632 Caseins Human genes 0.000 abstract 1
- 108010076119 Caseins Proteins 0.000 abstract 1
- 229960000074 biopharmaceutical Drugs 0.000 abstract 1
- 238000010367 cloning Methods 0.000 abstract 1
- 239000013604 expression vector Substances 0.000 abstract 1
- 230000001605 fetal effect Effects 0.000 abstract 1
- 210000002950 fibroblast Anatomy 0.000 abstract 1
- 230000002068 genetic effect Effects 0.000 abstract 1
- 244000144980 herd Species 0.000 abstract 1
- 210000005075 mammary gland Anatomy 0.000 abstract 1
- 239000000463 material Substances 0.000 abstract 1
- 210000000287 oocyte Anatomy 0.000 abstract 1
- 238000000746 purification Methods 0.000 abstract 1
- 210000001082 somatic cell Anatomy 0.000 abstract 1
- 238000001890 transfection Methods 0.000 abstract 1
- 235000021247 β-casein Nutrition 0.000 abstract 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/027—New or modified breeds of vertebrates
- A01K67/0275—Genetically modified vertebrates, e.g. transgenic
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/61—Growth hormone [GH], i.e. somatotropin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
- C12N15/877—Techniques for producing new mammalian cloned embryos
- C12N15/8771—Bovine embryos
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
- A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2227/00—Animals characterised by species
- A01K2227/10—Mammal
- A01K2227/101—Bovine
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2267/00—Animals characterised by purpose
- A01K2267/01—Animal expressing industrially exogenous proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/008—Vector systems having a special element relevant for transcription cell type or tissue specific enhancer/promoter combination
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Endocrinology (AREA)
- Environmental Sciences (AREA)
- Microbiology (AREA)
- Veterinary Medicine (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Developmental Biology & Embryology (AREA)
- Toxicology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Animal Behavior & Ethology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Medicinal Chemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Abstract
Un método para producir una proteína de interés, que comprende la creacion de un mamífero transgénico no humano capaz de producir dicha proteína en su leche, la obtencion de dicha leche del mamífero transgénico no humano y la purificacion de dicha proteína de interés a partir de la leche. Se generaron animales bovinos transgénicos, que son capaces de producir hormona de crecimiento humana en sus glándulas mamarias. El método involucra el clonado de una construccion genética que codifica para el gen de hGh y el promotor de la beta caseína convenientemente ensamblados en un vector de expresion. También incluye los procedimientos de transfeccion de células somáticas fetales bovinas, generalmente fibroblastos, y la transferencia nuclear a ovocitos bovinos enucleados, generando así embriones transgénicos. El método también incluye otros procedimientos para generar embriones transgénicos para ulterior expansion del rebano transgénico, tales como el subclonado de hembras bovinas transgénicas, la superovulacion de vacas transgénicas y su inseminacion con semen de un macho bovino transgénico o no transgénico, y al superovulacion de vacas no transgénicas y su inseminacion con semen obtenido de un macho bovino transgénico. Posteriormente, los embriones transgénicos darán origen a ganado transgénico productor de grandes cantidades de hormona de crecimiento humana en su leche, material a partir del cual ésta se purifica en forma completa y se somete a los análisis que permitan garantizar el cumplimiento de todos los requisitos necesarios para la fabricacion de una producto biofarmacéutico seguro y eficaz.
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US50673503P | 2003-09-30 | 2003-09-30 | |
| US50673603P | 2003-09-30 | 2003-09-30 | |
| US55602604P | 2004-03-25 | 2004-03-25 | |
| US55602704P | 2004-03-25 | 2004-03-25 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR047721A1 true AR047721A1 (es) | 2006-02-15 |
Family
ID=34427057
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP040103538A AR047720A1 (es) | 2003-09-30 | 2004-09-29 | Un proceso para la obtencion de mamiferos transgenicos que produzcan proteinas exogenas en leche y los mamiferos producidos por dicho proceso |
| ARP040103539A AR047721A1 (es) | 2003-09-30 | 2004-09-29 | Un proceso para producir proteina exogena en la leche de mamiferos transgenicos y un proceso para purificar proteinas a partir de ella |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP040103538A AR047720A1 (es) | 2003-09-30 | 2004-09-29 | Un proceso para la obtencion de mamiferos transgenicos que produzcan proteinas exogenas en leche y los mamiferos producidos por dicho proceso |
Country Status (9)
| Country | Link |
|---|---|
| US (5) | US7718845B2 (es) |
| EP (2) | EP1685245A4 (es) |
| JP (2) | JP2007507510A (es) |
| AR (2) | AR047720A1 (es) |
| AU (2) | AU2004278747B2 (es) |
| BR (2) | BRPI0415146A (es) |
| CA (2) | CA2540667A1 (es) |
| MX (1) | MXPA06003553A (es) |
| WO (2) | WO2005033274A2 (es) |
Families Citing this family (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2540667A1 (en) * | 2003-09-30 | 2005-04-14 | Sterrenbeld Biotechnologie North America, Inc. | A process for producing exogenous protein in the milk of trangenic mammals and a process for purifying proteins therefrom |
| WO2006057466A1 (en) * | 2004-11-23 | 2006-06-01 | Korea Research Institute Of Bioscience And Biotechnology | Beta-casein gene targeting vector using homologous recombination |
| GB2438760A (en) * | 2004-12-22 | 2007-12-05 | Ambrx Inc | Methods for expression and purification of recombinant human growth hormone |
| US20070067842A1 (en) * | 2005-08-08 | 2007-03-22 | Greene Michael P | Systems and methods for collecting files related to malware |
| US8383125B2 (en) | 2006-03-23 | 2013-02-26 | Agriculture Victoria Services Pty, Limited | Antimicrobial protein |
| CN1970750B (zh) * | 2006-12-11 | 2010-10-13 | 中国人民解放军军事医学科学院军事兽医研究所 | 一种利用抗菌肽培育具有抗乳房炎病奶牛重构胚的方法 |
| RU2010100911A (ru) * | 2007-06-13 | 2011-07-20 | Стэрнбэлт Байэутекнолэджай Нос Эмерикэ, Инк. (US) | Плазмида, содержащая нуклеотидную последовательность, кодирующую модифицированный предшественник инсулина, и способ ее трансфекции в клетки млекопитающего, трансгенное млекопитающее, продуцирующее указанный предшественник, и способ его получения |
| EP2242478A2 (en) * | 2008-01-02 | 2010-10-27 | Kringle Pharma Inc. | Topical compositions for delivery of proteins and peptides |
| WO2009111838A1 (en) * | 2008-03-13 | 2009-09-17 | Agriculture Victoria Services Pty Limited | Method of treatment using antimicrobial composition |
| US9238878B2 (en) | 2009-02-17 | 2016-01-19 | Redwood Bioscience, Inc. | Aldehyde-tagged protein-based drug carriers and methods of use |
| AU2012205301B2 (en) | 2011-01-14 | 2017-01-05 | Redwood Bioscience, Inc. | Aldehyde-tagged immunoglobulin polypeptides and method of use thereof |
| JP2012210199A (ja) * | 2011-03-31 | 2012-11-01 | National Agriculture & Food Research Organization | 体外発育卵母細胞をレシピエント卵子とするクローン動物個体の作成法 |
| CN102628045A (zh) * | 2012-04-09 | 2012-08-08 | 西北农林科技大学 | 一种干扰BVDV复制的shRNA表达载体及构建的重组细胞 |
| KR101504824B1 (ko) | 2013-05-31 | 2015-03-23 | 씨제이헬스케어 주식회사 | 고당화된 지속형 인간 성장호르몬 단백질 및 이의 제조방법 |
| WO2013183948A1 (ko) * | 2012-06-05 | 2013-12-12 | 씨제이제일제당 (주) | 고당화된 지속형 인간 성장호르몬 단백질 및 이의 제조방법 |
| FR3025515B1 (fr) | 2014-09-05 | 2016-09-09 | Lab Francais Du Fractionnement | Procede de purification d'un anticorps monoclonal |
| FR3034420A1 (fr) | 2015-03-31 | 2016-10-07 | Lab Francais Du Fractionnement | Anticorps monoclonaux anti-cd303 |
| CN109071634A (zh) | 2016-04-26 | 2018-12-21 | R.P.谢勒技术有限责任公司 | 抗体偶联物及其制备和使用方法 |
| FR3060394B1 (fr) | 2016-12-16 | 2019-05-24 | Laboratoire Francais Du Fractionnement Et Des Biotechnologies | Combinaison d'anticorps anti-cd303 et anti-amhrii |
| FR3060395B1 (fr) | 2016-12-16 | 2019-05-24 | Laboratoire Francais Du Fractionnement Et Des Biotechnologies | Combinaison d'anticorps anti-cd303 et anti-her2 |
| FR3076294B1 (fr) | 2017-12-29 | 2022-01-28 | Lab Francais Du Fractionnement | Procede de purification d'anticorps a partir de lait brut |
| CA3223956A1 (en) | 2021-12-20 | 2023-06-29 | Vetoquinol Sa | Anti-canine interleukine-31-receptor a (il-31ra) antibodies and the uses thereof |
Family Cites Families (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4371462A (en) * | 1982-01-21 | 1983-02-01 | Genex Corporation | Method for purification of anterior pituitary hormones |
| JPH04506751A (ja) | 1989-09-11 | 1992-11-26 | ティー エスアイ コーポレーション | 遺伝子導入動物の乳汁中での成長ホルモンの生産 |
| US5633076A (en) * | 1989-12-01 | 1997-05-27 | Pharming Bv | Method of producing a transgenic bovine or transgenic bovine embryo |
| US5160312A (en) * | 1990-02-09 | 1992-11-03 | W. R. Grace & Co.-Conn. | Cryopreservation process for direct transfer of embryos |
| US5827690A (en) | 1993-12-20 | 1998-10-27 | Genzyme Transgenics Corporatiion | Transgenic production of antibodies in milk |
| US6011197A (en) * | 1997-03-06 | 2000-01-04 | Infigen, Inc. | Method of cloning bovines using reprogrammed non-embryonic bovine cells |
| AU702218B2 (en) * | 1997-03-31 | 1999-02-18 | Kyushu-Taiyoukasei., Ltd. | Liner film for bulk container and container liner |
| US6451527B1 (en) * | 1997-08-29 | 2002-09-17 | Selective Genetics, Inc. | Methods using genetic package display for selecting internalizing ligands for gene delivery |
| CA2259368C (en) * | 1998-01-16 | 2010-10-05 | Agrobiogen Gmbh | Efficient nucleus transfer with foetal fibroblasts |
| GB9807464D0 (en) | 1998-04-07 | 1998-06-10 | Pharming Bv | Purification of human acid µ-glucosidase |
| US20030005468A1 (en) * | 1998-06-19 | 2003-01-02 | Meade Harry M. | Methods and vectors for improving nucleic acid expression |
| EP1090132A1 (en) * | 1998-06-26 | 2001-04-11 | PPL Therapeutics (Scotland) Limited | Methods for production of recombinant polypeptides |
| JP2001197846A (ja) * | 2000-01-18 | 2001-07-24 | Ys New Technology Kenkyusho:Kk | 免疫寛容動物の作製方法 |
| MXPA03004313A (es) * | 2000-11-17 | 2007-02-27 | Hematech Llc | Expresion de inmunoglobulinas xenogenas (humanas) en ungulados transgenicos, clonados. |
| US7105314B2 (en) * | 2001-04-02 | 2006-09-12 | Novo Nordisk A/S | Method for making human insulin precursors |
| AUPR451401A0 (en) * | 2001-04-20 | 2001-05-24 | Monash University | A method of nuclear transfer |
| AU2002364596A1 (en) * | 2001-12-21 | 2003-07-15 | Immerge Biotherapeutics, Inc. | Knockout swine and methods for making the same |
| CA2540667A1 (en) * | 2003-09-30 | 2005-04-14 | Sterrenbeld Biotechnologie North America, Inc. | A process for producing exogenous protein in the milk of trangenic mammals and a process for purifying proteins therefrom |
-
2004
- 2004-09-29 CA CA002540667A patent/CA2540667A1/en not_active Abandoned
- 2004-09-29 WO PCT/US2004/031819 patent/WO2005033274A2/en active Application Filing
- 2004-09-29 AR ARP040103538A patent/AR047720A1/es unknown
- 2004-09-29 US US10/952,377 patent/US7718845B2/en not_active Expired - Fee Related
- 2004-09-29 EP EP04789272A patent/EP1685245A4/en not_active Withdrawn
- 2004-09-29 MX MXPA06003553A patent/MXPA06003553A/es active IP Right Grant
- 2004-09-29 BR BRPI0415146-1A patent/BRPI0415146A/pt not_active IP Right Cessation
- 2004-09-29 AR ARP040103539A patent/AR047721A1/es active IP Right Grant
- 2004-09-29 JP JP2006534022A patent/JP2007507510A/ja active Pending
- 2004-09-29 JP JP2006534064A patent/JP2007507232A/ja active Pending
- 2004-09-29 WO PCT/US2004/032019 patent/WO2005033281A2/en active Application Filing
- 2004-09-29 US US10/952,376 patent/US7807862B2/en not_active Expired - Fee Related
- 2004-09-29 BR BRPI0415147-0A patent/BRPI0415147A/pt not_active Application Discontinuation
- 2004-09-29 AU AU2004278747A patent/AU2004278747B2/en not_active Ceased
- 2004-09-29 AU AU2004278763A patent/AU2004278763A1/en not_active Abandoned
- 2004-09-29 CA CA002540854A patent/CA2540854A1/en not_active Abandoned
- 2004-09-29 EP EP04789169.2A patent/EP1687421B1/en not_active Expired - Fee Related
-
2007
- 2007-07-11 US US11/822,985 patent/US20080060089A1/en not_active Abandoned
- 2007-07-11 US US11/822,984 patent/US20080229438A1/en not_active Abandoned
-
2010
- 2010-05-17 US US12/781,500 patent/US20110072526A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| EP1685245A2 (en) | 2006-08-02 |
| BRPI0415147A (pt) | 2006-11-28 |
| US20050177878A1 (en) | 2005-08-11 |
| AR047720A1 (es) | 2006-02-15 |
| WO2005033274A3 (en) | 2005-07-14 |
| AU2004278747A1 (en) | 2005-04-14 |
| CA2540667A1 (en) | 2005-04-14 |
| WO2005033281A2 (en) | 2005-04-14 |
| JP2007507510A (ja) | 2007-03-29 |
| US20080229438A1 (en) | 2008-09-18 |
| AU2004278763A1 (en) | 2005-04-14 |
| US20110072526A1 (en) | 2011-03-24 |
| US7807862B2 (en) | 2010-10-05 |
| US7718845B2 (en) | 2010-05-18 |
| AU2004278747B2 (en) | 2011-04-28 |
| US20050177879A1 (en) | 2005-08-11 |
| EP1687421A4 (en) | 2006-12-20 |
| BRPI0415146A (pt) | 2006-11-28 |
| EP1687421A2 (en) | 2006-08-09 |
| US20080060089A1 (en) | 2008-03-06 |
| WO2005033274A2 (en) | 2005-04-14 |
| CA2540854A1 (en) | 2005-04-14 |
| EP1685245A4 (en) | 2007-01-03 |
| MXPA06003553A (es) | 2007-02-02 |
| EP1687421B1 (en) | 2018-06-27 |
| JP2007507232A (ja) | 2007-03-29 |
| WO2005033281A3 (en) | 2005-05-19 |
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