BRPI0415146A - plasmìdio, fragmento linear do plasmìdio, método para a transfecção de construtos genéticos, mamìfero transgênico não humano, mamìfero não transgênico não humano, mamìfero transgênico não humano da espécie bovina e método para a produção de um mamìfero transgênico não humano - Google Patents

plasmìdio, fragmento linear do plasmìdio, método para a transfecção de construtos genéticos, mamìfero transgênico não humano, mamìfero não transgênico não humano, mamìfero transgênico não humano da espécie bovina e método para a produção de um mamìfero transgênico não humano

Info

Publication number
BRPI0415146A
BRPI0415146A BRPI0415146-1A BRPI0415146A BRPI0415146A BR PI0415146 A BRPI0415146 A BR PI0415146A BR PI0415146 A BRPI0415146 A BR PI0415146A BR PI0415146 A BRPI0415146 A BR PI0415146A
Authority
BR
Brazil
Prior art keywords
transgenic
human
transgenic mammal
plasmid
producing
Prior art date
Application number
BRPI0415146-1A
Other languages
English (en)
Inventor
Carlos Alberto Melo
Lino Baranao
Original Assignee
Sterrenbeld Biotechnologie North America Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sterrenbeld Biotechnologie North America Inc filed Critical Sterrenbeld Biotechnologie North America Inc
Publication of BRPI0415146A publication Critical patent/BRPI0415146A/pt

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
    • A01K67/027—New breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormones [GH] (Somatotropin)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
    • C12N15/877—Techniques for producing new mammalian cloned embryos
    • C12N15/8771—Bovine embryos
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/101—Bovine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/01—Animal expressing industrially exogenous proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/008—Vector systems having a special element relevant for transcription cell type or tissue specific enhancer/promoter combination

Abstract

"PLASMìDIO, FRAGMENTO LINEAR DO PLASMìDIO, MéTODO PARA A TRANSFECçãO DE CONSTRUTOS GENéTICOS, MAMìFERO TRANSGêNICO NãO HUMANO, MAMìFERO NãO TRANSGêNICO NãO HUMANO, MAMìFERO TRANSGêNICO NãO HUMANO DA ESPéCIE BOVINA E MéTODO PARA A PRODUçãO DE UM MAMìFERO TRANSGêNICO NãO HUMANO". A invenção refere-se a um método para a produção de uma proteína de interesse, o qual compreende a produção de um mamífero transgênico não humano que produz a dita proteína em seu leite, a obtenção do dito leite do mamífero transgênico não humano e a purificação da dita proteína de interesse do leite. Animais bovinos transgênicos foram gerados, os quais podem produzir o hormónio do crescimento humano nas glândulas mamárias. O método envolve a clonagem de um construto genético que codifica o gene de hGH e um promotor de beta caseína convenientemente em um vetor de expressão. Ele também inclui procedimentos de transfecção em células somáticas do feto de bovinos, geralmente fibroblastos, e a transferência nuclear em oócitos de bovinos enucleados, dessa maneira gerando embriões transgênicos. O método também inclui outros procedimentos para a geração de embriões transgênicos para uma maior expansão do rebanho transgênico, tal como a subclonagem de fêmeas bovinas transgênicas, a superovulação de vacas transgênicas e a sua inseminação com sêmen ou de um macho bovino transgênico ou não transgênico, e a superovulação de vacas não transgênicas e a sua inseminação com sêmen de machos bovinos transgênicos. Em seguida, os embriões transgênicos resultam no gado transgênico que produz o hormónio do crescimento humano em grandes quantidades em seu leite, do qual o hormónio é completamente purificado e analisado para preencher todos os requisitos para a manufatura de um produto biofarmacêutico puro.
BRPI0415146-1A 2003-09-30 2004-09-29 plasmìdio, fragmento linear do plasmìdio, método para a transfecção de construtos genéticos, mamìfero transgênico não humano, mamìfero não transgênico não humano, mamìfero transgênico não humano da espécie bovina e método para a produção de um mamìfero transgênico não humano BRPI0415146A (pt)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US50673603P 2003-09-30 2003-09-30
US50673503P 2003-09-30 2003-09-30
US55602604P 2004-03-25 2004-03-25
US55602704P 2004-03-25 2004-03-25
PCT/US2004/032019 WO2005033281A2 (en) 2003-09-30 2004-09-29 A process of making transgenic mammals that produce exogenous proteins in milk and transgenic mammals produced thereby

Publications (1)

Publication Number Publication Date
BRPI0415146A true BRPI0415146A (pt) 2006-11-28

Family

ID=34427057

Family Applications (2)

Application Number Title Priority Date Filing Date
BRPI0415146-1A BRPI0415146A (pt) 2003-09-30 2004-09-29 plasmìdio, fragmento linear do plasmìdio, método para a transfecção de construtos genéticos, mamìfero transgênico não humano, mamìfero não transgênico não humano, mamìfero transgênico não humano da espécie bovina e método para a produção de um mamìfero transgênico não humano
BRPI0415147-0A BRPI0415147A (pt) 2003-09-30 2004-09-29 método para a produção de uma proteìna, método para a purificação de um hormÈnio de crescimento recombinante do leite de um mamìfero transgênico não humano que produz um hormÈnio de crescimento recombinante e método para a purificação de um hormÈnio de crescimento recombinante do leite de um mamìfero não transgênico que produz um hormÈnio de crescimento recombinante

Family Applications After (1)

Application Number Title Priority Date Filing Date
BRPI0415147-0A BRPI0415147A (pt) 2003-09-30 2004-09-29 método para a produção de uma proteìna, método para a purificação de um hormÈnio de crescimento recombinante do leite de um mamìfero transgênico não humano que produz um hormÈnio de crescimento recombinante e método para a purificação de um hormÈnio de crescimento recombinante do leite de um mamìfero não transgênico que produz um hormÈnio de crescimento recombinante

Country Status (9)

Country Link
US (5) US7807862B2 (pt)
EP (2) EP1687421B1 (pt)
JP (2) JP2007507510A (pt)
AR (2) AR047721A1 (pt)
AU (2) AU2004278763A1 (pt)
BR (2) BRPI0415146A (pt)
CA (2) CA2540854A1 (pt)
MX (1) MXPA06003553A (pt)
WO (2) WO2005033281A2 (pt)

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Also Published As

Publication number Publication date
WO2005033274A3 (en) 2005-07-14
BRPI0415147A (pt) 2006-11-28
EP1685245A2 (en) 2006-08-02
WO2005033281A2 (en) 2005-04-14
AR047721A1 (es) 2006-02-15
US20080229438A1 (en) 2008-09-18
CA2540667A1 (en) 2005-04-14
AU2004278747A1 (en) 2005-04-14
AU2004278763A1 (en) 2005-04-14
US20050177879A1 (en) 2005-08-11
AU2004278747B2 (en) 2011-04-28
US7807862B2 (en) 2010-10-05
JP2007507232A (ja) 2007-03-29
AR047720A1 (es) 2006-02-15
US7718845B2 (en) 2010-05-18
US20080060089A1 (en) 2008-03-06
CA2540854A1 (en) 2005-04-14
WO2005033281A3 (en) 2005-05-19
MXPA06003553A (es) 2007-02-02
US20110072526A1 (en) 2011-03-24
US20050177878A1 (en) 2005-08-11
EP1685245A4 (en) 2007-01-03
EP1687421B1 (en) 2018-06-27
EP1687421A2 (en) 2006-08-09
WO2005033274A2 (en) 2005-04-14
EP1687421A4 (en) 2006-12-20
JP2007507510A (ja) 2007-03-29

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B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: REFERENTE AO DESPACHO 8.6 PUBLICADO NA RPI 2158 DE 15/05/2012.