AR015499A1 - Metodo para aumentar el rendimiento de una proteina e2 o erns de pestivirus recombinante o fragmentos inmunogenicos de los mismos o de fsh producido en cultivo celular de insecto, la utilizacion de dicho metodo para producir una sustancia antigenica y una vacuna - Google Patents
Metodo para aumentar el rendimiento de una proteina e2 o erns de pestivirus recombinante o fragmentos inmunogenicos de los mismos o de fsh producido en cultivo celular de insecto, la utilizacion de dicho metodo para producir una sustancia antigenica y una vacunaInfo
- Publication number
- AR015499A1 AR015499A1 ARP980106452A ARP980106452A AR015499A1 AR 015499 A1 AR015499 A1 AR 015499A1 AR P980106452 A ARP980106452 A AR P980106452A AR P980106452 A ARP980106452 A AR P980106452A AR 015499 A1 AR015499 A1 AR 015499A1
- Authority
- AR
- Argentina
- Prior art keywords
- protein
- produce
- insect
- cell culture
- increase
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 5
- 241000238631 Hexapoda Species 0.000 title abstract 4
- 108090000623 proteins and genes Proteins 0.000 title abstract 4
- 102000004169 proteins and genes Human genes 0.000 title abstract 4
- 238000004113 cell culture Methods 0.000 title abstract 3
- 239000012634 fragment Substances 0.000 title abstract 3
- 241000710778 Pestivirus Species 0.000 title 1
- 230000002163 immunogen Effects 0.000 title 1
- 241000701447 unidentified baculovirus Species 0.000 abstract 3
- 239000001963 growth medium Substances 0.000 abstract 2
- 239000000126 substance Substances 0.000 abstract 2
- 101710125507 Integrase/recombinase Proteins 0.000 abstract 1
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 abstract 1
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 abstract 1
- 230000000890 antigenic effect Effects 0.000 abstract 1
- 238000012258 culturing Methods 0.000 abstract 1
- 239000002054 inoculum Substances 0.000 abstract 1
- 239000002609 medium Substances 0.000 abstract 1
- 229960005486 vaccine Drugs 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P15/00—Drugs for genital or sexual disorders; Contraceptives
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
- A61P31/14—Antivirals for RNA viruses
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/14011—Baculoviridae
- C12N2710/14111—Nucleopolyhedrovirus, e.g. autographa californica nucleopolyhedrovirus
- C12N2710/14141—Use of virus, viral particle or viral elements as a vector
- C12N2710/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
- C12N2770/00011—Details
- C12N2770/24011—Flaviviridae
- C12N2770/24311—Pestivirus, e.g. bovine viral diarrhea virus
- C12N2770/24322—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
- C12N2770/00011—Details
- C12N2770/24011—Flaviviridae
- C12N2770/24311—Pestivirus, e.g. bovine viral diarrhea virus
- C12N2770/24334—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Virology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Animal Behavior & Ethology (AREA)
- Physics & Mathematics (AREA)
- General Chemical & Material Sciences (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Pharmacology & Pharmacy (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Communicable Diseases (AREA)
- Oncology (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Endocrinology (AREA)
- Reproductive Health (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Abstract
Método para la expresion de proteínas en los sistemas de expresion vector baculovirus para incrementar el rendimiento de una proteína recombinanteproducida en un cultivo de células de insecto, que comprende seleccionar un baculovirus recombinanteque exprese dicha proteína, cultivar células de insectosen un medio de cultivo en un recipiente de cultivo con un suficiente volumen para contener por lo menos 2 litros, e infectar las células con un inoculo de porlo menos un baculovirus con unadensida d de células de 1 x 10E5 a 5 x 10E6 células / ml con una m.o.i. de < 0,01; método para producir proteína E2 o Erns depestivirus recombinante, o fragmentos de la misma, en un cultivo de células de insecto, por una concentracion final de dichaproteína (fragmentos) en el mediode cultivo en el medio de la recoleccion, de por lo menos 100 microg / ml; ell uso de dicho método para producir una sustancia antigénica y una vacuna quecomprende dicha sustancia.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP97203989A EP0924298A1 (en) | 1997-12-18 | 1997-12-18 | Protein expression in baculovirus vector expression systems |
Publications (1)
Publication Number | Publication Date |
---|---|
AR015499A1 true AR015499A1 (es) | 2001-05-02 |
Family
ID=8229076
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ARP980106452A AR015499A1 (es) | 1997-12-18 | 1998-12-17 | Metodo para aumentar el rendimiento de una proteina e2 o erns de pestivirus recombinante o fragmentos inmunogenicos de los mismos o de fsh producido en cultivo celular de insecto, la utilizacion de dicho metodo para producir una sustancia antigenica y una vacuna |
Country Status (22)
Country | Link |
---|---|
US (2) | US6555346B1 (es) |
EP (2) | EP0924298A1 (es) |
JP (2) | JP4220126B2 (es) |
KR (3) | KR100682171B1 (es) |
CN (2) | CN101130073A (es) |
AR (1) | AR015499A1 (es) |
AT (1) | ATE324456T1 (es) |
AU (1) | AU750514B2 (es) |
BR (1) | BR9813692B1 (es) |
CA (2) | CA2315248C (es) |
CO (1) | CO4820445A1 (es) |
DE (1) | DE69834346T2 (es) |
DK (1) | DK1049788T3 (es) |
ES (1) | ES2267203T3 (es) |
HK (1) | HK1037670A1 (es) |
HU (1) | HU228122B1 (es) |
IL (2) | IL136815A0 (es) |
NZ (1) | NZ505322A (es) |
TW (1) | TWI225094B (es) |
UY (1) | UY25315A1 (es) |
WO (1) | WO1999031257A2 (es) |
ZA (1) | ZA9811434B (es) |
Families Citing this family (98)
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AUPQ233799A0 (en) | 1999-08-19 | 1999-09-09 | Minister For Agriculture, Minister For Land And Water Conservation For And On Behalf Of The State Of New South Wales | Recombinant sub-unit vaccine |
CN1338310A (zh) * | 2000-08-10 | 2002-03-06 | 清华大学 | 一种猪瘟病毒表位疫苗及其制备方法 |
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CN109182380B (zh) * | 2018-08-14 | 2022-06-03 | 浙江大学 | 杆状病毒表达的猪瘟e2亚单位疫苗的制备方法及应用 |
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ZA901719B (en) * | 1989-03-19 | 1991-01-30 | Akzo Nv | Hog cholera virus vaccine and diagnostics |
US5514634A (en) | 1991-11-06 | 1996-05-07 | Mobil Oil Corporation | High activity polyethylene catalysts |
EP0766740A1 (en) * | 1994-06-17 | 1997-04-09 | Instituut Voor Dierhouderij En Diergezondheid (Id-Dlo) | Nucleotide sequences of pestivirus strains, polypeptides encoded by these sequences and use thereof for diagnosis and prevention of pestivirus infections |
DK0772632T3 (da) * | 1994-12-20 | 2001-12-31 | Akzo Nobel Nv | T-celle-stimulerende protein af pestivirus |
AU720337B2 (en) * | 1995-02-17 | 2000-05-25 | Stichting Dienst Landbouwkundig Onderzoek | Production of biologically active recombinant bovine follicle stimulating hormone (REC bFSH) in the baculovirus expression system |
EP0924298A1 (en) | 1997-12-18 | 1999-06-23 | Stichting Instituut voor Dierhouderij en Diergezondheid (ID-DLO) | Protein expression in baculovirus vector expression systems |
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