ZA200610223B - Non-recombinant Saccharomyces strains that grow on xylose - Google Patents
Non-recombinant Saccharomyces strains that grow on xylose Download PDFInfo
- Publication number
- ZA200610223B ZA200610223B ZA200610223A ZA200610223A ZA200610223B ZA 200610223 B ZA200610223 B ZA 200610223B ZA 200610223 A ZA200610223 A ZA 200610223A ZA 200610223 A ZA200610223 A ZA 200610223A ZA 200610223 B ZA200610223 B ZA 200610223B
- Authority
- ZA
- South Africa
- Prior art keywords
- strain
- xylose
- test
- saccharomyces
- yeast cells
- Prior art date
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- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 title claims abstract description 915
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 title claims abstract description 460
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 title claims abstract description 460
- 241000235070 Saccharomyces Species 0.000 title claims abstract description 221
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
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- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
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- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
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AU2004903141A AU2004903141A0 (en) | 2004-06-08 | Yeast |
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ZA200610223A ZA200610223B (en) | 2004-06-08 | 2006-12-06 | Non-recombinant Saccharomyces strains that grow on xylose |
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US (4) | US8257959B2 (fr) |
EP (1) | EP1766007B1 (fr) |
JP (2) | JP5826447B2 (fr) |
CN (1) | CN1977042B (fr) |
AT (1) | ATE527352T1 (fr) |
AU (1) | AU2005252266B8 (fr) |
BR (1) | BRPI0511921B1 (fr) |
CA (1) | CA2567849C (fr) |
DK (1) | DK1766007T3 (fr) |
EA (1) | EA010892B1 (fr) |
ES (1) | ES2373397T3 (fr) |
MX (1) | MXPA06014165A (fr) |
NZ (1) | NZ552213A (fr) |
PL (1) | PL1766007T3 (fr) |
WO (1) | WO2005121337A1 (fr) |
ZA (1) | ZA200610223B (fr) |
Families Citing this family (43)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ATE527352T1 (de) * | 2004-06-08 | 2011-10-15 | Microbiogen Pty Ltd | Nichtrekombinante, auf xylose wachsende saccharomyces-stämme |
WO2009100042A2 (fr) * | 2008-02-05 | 2009-08-13 | Syngenta Participations Ag | Systèmes et procédés de production de biocarburants à partir de la biomasse |
CA2726054A1 (fr) * | 2008-06-27 | 2009-12-30 | Microbiogen Pty Ltd. | Procede de fabrication de biomasse de levure |
KR101569210B1 (ko) * | 2008-10-29 | 2015-11-23 | 삼성전자주식회사 | 갈락토오스의 대사 이용율을 증대시키는 유전자, 이를 포함하는 재조합 벡터 및 재조합 미생물 |
JP5850418B2 (ja) * | 2010-12-03 | 2016-02-03 | 国立研究開発法人産業技術総合研究所 | キシロース代謝改変による効果的なエタノール生産法 |
US9850512B2 (en) | 2013-03-15 | 2017-12-26 | The Research Foundation For The State University Of New York | Hydrolysis of cellulosic fines in primary clarified sludge of paper mills and the addition of a surfactant to increase the yield |
CN103320333B (zh) * | 2013-05-16 | 2014-12-10 | 大连理工大学 | 携带染色体分散整合木糖基因的工业酿酒酵母重组菌株 |
JP6221478B2 (ja) * | 2013-08-02 | 2017-11-01 | 三菱ケミカル株式会社 | ペントース資化能を有する微生物を用いた有機化合物の製造方法 |
JP6210487B2 (ja) * | 2013-10-11 | 2017-10-11 | 国立大学法人山口大学 | キシロース資化能を有するエタノール生産酵母を生産する方法 |
WO2015057148A1 (fr) * | 2013-10-16 | 2015-04-23 | Scandinavian Technology Group Ab | Souches de saccharomyces cerevisiae |
FR3017623B1 (fr) * | 2014-02-17 | 2018-08-10 | Lesaffre Et Compagnie | Souche fermentant les pentoses a propagation optimisee |
US9951363B2 (en) | 2014-03-14 | 2018-04-24 | The Research Foundation for the State University of New York College of Environmental Science and Forestry | Enzymatic hydrolysis of old corrugated cardboard (OCC) fines from recycled linerboard mill waste rejects |
BR112016024470A2 (pt) | 2014-04-23 | 2018-01-23 | Scandinavian Tech Group Ab | cepa de saccharomyces cerevisiae, e, uso de uma cepa de saccharomyces cerevisiae |
CN104371937B (zh) * | 2014-10-30 | 2017-07-14 | 江南大学 | 一株能以多碳源共发酵的酿酒酵母及其应用 |
AU2015371749B2 (en) | 2014-12-23 | 2019-11-07 | Carlsberg Breweries A/S | Yeast for preparing alcoholic beverages |
AU2016225049B2 (en) | 2015-02-27 | 2022-03-31 | Microbiogen Pty. Ltd. | Processes of producing ethanol using a fermenting organism |
BR112018015184B1 (pt) | 2016-02-19 | 2022-09-06 | Intercontinental Great Brands Llc | Processos para criar múltiplas correntes de valor a partir de fontes de biomassa |
WO2018098381A1 (fr) | 2016-11-23 | 2018-05-31 | Novozymes A/S | Levure améliorée pour la production d'éthanol |
US20190345522A1 (en) | 2016-12-06 | 2019-11-14 | Novozymes A/S | Improved Processes For Production Of Ethanol From Xylose-Containing Cellulosic Substrates Using Engineered Yeast Strains |
WO2018220116A1 (fr) | 2017-05-31 | 2018-12-06 | Novozymes A/S | Souches de levure fermentant le xylose et procédés d'utilisation de celles-ci pour la production d'éthanol |
BR112019025391A2 (pt) | 2017-06-02 | 2020-07-07 | Novozymes A/S | levedura melhorada para a produção de etanol |
CN113286871A (zh) | 2018-01-29 | 2021-08-20 | 诺维信公司 | 用于乙醇生产的氮利用提高的微生物 |
AU2019222480A1 (en) | 2018-02-15 | 2020-10-08 | Microbiogen Pty. Ltd. | Improved yeast for ethanol production |
WO2019231944A2 (fr) | 2018-05-31 | 2019-12-05 | Novozymes A/S | Procédés d'amélioration de la croissance et de la productivité de levures |
CN112601818A (zh) | 2018-07-25 | 2021-04-02 | 诺维信公司 | 用于生产乙醇的表达酶的酵母 |
US11807889B2 (en) | 2018-10-08 | 2023-11-07 | Novozymes A/S | Yeast expressing a heterologous phospholipase for ethanol production |
CN114450390A (zh) | 2019-07-26 | 2022-05-06 | 诺维信公司 | 用于乙醇生产的氮转运提高的微生物 |
CA3144423A1 (fr) | 2019-08-05 | 2021-02-11 | Kurt Creamer | Melanges d'enzymes et procedes de production d'un ingredient d'alimentation animale a haute teneur en proteines a partir d'un sous-produit de type residu de distillation entier |
WO2021025872A1 (fr) | 2019-08-06 | 2021-02-11 | Novozymes A/S | Protéines de fusion pour une expression enzymatique améliorée |
MX2022002834A (es) | 2019-09-16 | 2022-04-06 | Novozymes As | Polipeptidos con actividad beta-glucanasa y polinucleotidos que los codifican. |
BR112022011271A2 (pt) | 2019-12-10 | 2022-09-06 | Novozymes As | Célula hospedeira recombinante, composição, métodos para produzir um derivado de uma célula e um produto de fermentação, e, uso de uma célula hospedeira recombinante |
US20240228996A1 (en) | 2020-02-10 | 2024-07-11 | Novozymes A/S | Polypeptides having alpha-amylase activity and polynucleotides encoding same |
MX2023002490A (es) | 2020-09-04 | 2023-03-09 | Novozymes As | Organismo fermentador mejorado para la produccion de etanol. |
AU2021370998A1 (en) | 2020-11-02 | 2023-05-25 | Novozymes A/S | Glucoamylase variants and polynucleotides encoding same |
CN112725208B (zh) * | 2021-03-05 | 2022-08-09 | 伽蓝(集团)股份有限公司 | 西藏来源的在低温下产油脂的酵母菌株及其应用 |
CN117795089A (zh) | 2021-06-07 | 2024-03-29 | 诺维信公司 | 用于改善的乙醇发酵的工程化微生物 |
WO2023164436A1 (fr) | 2022-02-23 | 2023-08-31 | Novozymes A/S | Procédé pour produire des produits de fermentation et du biogaz à partir de matériaux contenant de l'amidon |
WO2024064901A2 (fr) | 2022-09-23 | 2024-03-28 | Novozymes A/S | Organisme de fermentation améliorée de production d'éthanol |
WO2024137704A2 (fr) | 2022-12-19 | 2024-06-27 | Novozymes A/S | Procédés de production de produits de fermentation faisant appel à des enzymes de dégradation de fibres avec levure modifiée |
WO2024137248A1 (fr) | 2022-12-19 | 2024-06-27 | Novozymes A/S | Compositions contenant des arabinofuranosidases et une xylanase, et leur utilisation pour augmenter la solubilisation de fibres hémicellulosiques |
WO2024137250A1 (fr) | 2022-12-19 | 2024-06-27 | Novozymes A/S | Polypeptides de la famille 3 de gludice estérase (ce3) présentant une activité acétyl xylane estérase et polynucléotides codant pour ceux-ci |
WO2024137252A1 (fr) | 2022-12-19 | 2024-06-27 | Novozymes A/S | Procédé de réduction de la viscosité du sirop à la fin d'un processus de production d'un produit de fermentation |
WO2024137246A1 (fr) | 2022-12-19 | 2024-06-27 | Novozymes A/S | Polypeptides de la famille 1 d'estérase de glucide (ce1) présentant une activité d'estérase d'acide férulique et/ou d'estérase d'acétyl xylane et polynucléotides codant pour ceux-ci |
Family Cites Families (23)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB1590830A (en) | 1976-12-24 | 1981-06-10 | Lesaffre & Cie | Strains of yeast for bread-making and a process for the obtention thereof |
JPS5941716B2 (ja) | 1977-08-24 | 1984-10-09 | 味の素株式会社 | 酵母菌体の製造法 |
US4368268A (en) | 1981-05-15 | 1983-01-11 | Purdue Research Foundation | Direct fermentation of D-xylose to ethanol by a xylose-fermenting yeast mutant |
US4511656A (en) | 1981-05-15 | 1985-04-16 | Purdue Research Foundation | Direct fermentation of D-xylose to ethanol by a xylose-fermenting yeast mutant |
JPS61242584A (ja) * | 1985-04-20 | 1986-10-28 | Agency Of Ind Science & Technol | 微生物によるエタノ−ルの製造法 |
JPS6265679A (ja) * | 1986-07-19 | 1987-03-24 | Agency Of Ind Science & Technol | 新規なサツカロマイセス・セルビシエir−2株 |
JPS6465679A (en) | 1987-09-07 | 1989-03-10 | Oki Electric Ind Co Ltd | On-line character recognizing device |
FR2675815B1 (fr) | 1991-04-23 | 1994-11-04 | Lesaffre & Cie | Nouvelles souches de levure de panification et leur procede d'obtention, nouvelles levures fraiche et seche correspondantes. |
JPH06178682A (ja) | 1992-12-12 | 1994-06-28 | Hakutsuru Shuzo Kk | 寡酸性の清酒酵母の育種方法とその酵母菌株およびそれを用いた清酒の製造方法 |
EP1015554A4 (fr) | 1997-01-16 | 2002-02-06 | Univ California | Cultures de levures de vinification ameliorees |
DK1717322T3 (da) | 1997-01-17 | 2012-10-22 | Codexis Mayflower Holdings Llc | Udvikling af hele celler og organismer ved rekursiv sekvensrekombination |
US6326204B1 (en) * | 1997-01-17 | 2001-12-04 | Maxygen, Inc. | Evolution of whole cells and organisms by recursive sequence recombination |
US6071729A (en) | 1998-04-02 | 2000-06-06 | Jeffries; Thomas W. | Disruption of the cytochrome C gene in xylose-fermenting yeast |
US7033781B1 (en) | 1999-09-29 | 2006-04-25 | Diversa Corporation | Whole cell engineering by mutagenizing a substantial portion of a starting genome, combining mutations, and optionally repeating |
WO2001088094A1 (fr) | 2000-05-15 | 2001-11-22 | Forskarpatent I Syd | Levure recombinee destinee a des matieres premieres lignocellulosiques |
RU2186846C2 (ru) | 2000-09-18 | 2002-08-10 | Государственное унитарное предприятие "Государственный научно-исследовательский институт биосинтеза белковых веществ" | Штамм дрожжей saccharomyces cerevisiae, используемый для сбраживания мелассного сусла в производстве этилового спирта и хлебопекарных дрожжей |
RU2208630C1 (ru) | 2001-10-15 | 2003-07-20 | Ковальский Юрий Викторович | Способ получения кормового белкового продукта |
JP2003250523A (ja) * | 2002-02-26 | 2003-09-09 | Mercian Corp | エタノール生産性および酢酸イソアミル生産性に優れた酵母の育種方法 |
US7253001B2 (en) | 2002-03-19 | 2007-08-07 | Forskarpatent I Syd Ab | Metabolic engineering for improved xylose utilisation of Saccharomyces cerevisiae |
ATE527352T1 (de) | 2004-06-08 | 2011-10-15 | Microbiogen Pty Ltd | Nichtrekombinante, auf xylose wachsende saccharomyces-stämme |
PT103331A (pt) | 2005-08-05 | 2007-02-28 | Fundacao Da Faculdade De Cienc | Expressão dum transportador activo de xilose em saccharomyces cerevisiae modificada geneticamente |
BRPI0709946A2 (pt) | 2006-04-13 | 2011-08-02 | Ambrozea Inc | composições e métodos para produzir produtos e resìduos de fermentação |
CA2726054A1 (fr) | 2008-06-27 | 2009-12-30 | Microbiogen Pty Ltd. | Procede de fabrication de biomasse de levure |
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- 2005-06-08 PL PL05746690T patent/PL1766007T3/pl unknown
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- 2005-06-08 JP JP2007526108A patent/JP5826447B2/ja not_active Expired - Fee Related
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- 2005-06-08 MX MXPA06014165A patent/MXPA06014165A/es active IP Right Grant
- 2005-06-08 EP EP05746690A patent/EP1766007B1/fr active Active
- 2005-06-08 AU AU2005252266A patent/AU2005252266B8/en not_active Ceased
- 2005-06-08 BR BRPI0511921A patent/BRPI0511921B1/pt not_active IP Right Cessation
- 2005-06-08 US US11/570,329 patent/US8257959B2/en not_active Expired - Fee Related
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- 2005-06-08 CA CA2567849A patent/CA2567849C/fr not_active Expired - Fee Related
- 2005-06-08 CN CN200580021851.3A patent/CN1977042B/zh not_active Expired - Fee Related
- 2005-06-08 WO PCT/AU2005/000824 patent/WO2005121337A1/fr active Application Filing
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Also Published As
Publication number | Publication date |
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EP1766007A1 (fr) | 2007-03-28 |
BRPI0511921A (pt) | 2008-02-06 |
CA2567849A1 (fr) | 2005-12-22 |
DK1766007T3 (da) | 2012-01-09 |
US20130065270A1 (en) | 2013-03-14 |
US8735138B2 (en) | 2014-05-27 |
EP1766007A4 (fr) | 2008-03-12 |
PL1766007T3 (pl) | 2012-02-29 |
JP2008501348A (ja) | 2008-01-24 |
US20100068790A1 (en) | 2010-03-18 |
JP5826447B2 (ja) | 2015-12-02 |
EA200602194A1 (ru) | 2007-06-29 |
JP5872520B2 (ja) | 2016-03-01 |
US20150064772A1 (en) | 2015-03-05 |
NZ552213A (en) | 2009-09-25 |
JP2014054256A (ja) | 2014-03-27 |
CN1977042B (zh) | 2014-03-19 |
US20080009047A1 (en) | 2008-01-10 |
EA010892B1 (ru) | 2008-12-30 |
WO2005121337A1 (fr) | 2005-12-22 |
AU2005252266B2 (en) | 2010-10-28 |
US9556444B2 (en) | 2017-01-31 |
ATE527352T1 (de) | 2011-10-15 |
ES2373397T3 (es) | 2012-02-03 |
CA2567849C (fr) | 2017-01-03 |
EP1766007B1 (fr) | 2011-10-05 |
CN1977042A (zh) | 2007-06-06 |
AU2005252266B8 (en) | 2011-03-03 |
AU2005252266A1 (en) | 2005-12-22 |
US9249419B2 (en) | 2016-02-02 |
US8257959B2 (en) | 2012-09-04 |
MXPA06014165A (es) | 2007-04-25 |
BRPI0511921B1 (pt) | 2016-08-30 |
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