MX2023002490A - Organismo fermentador mejorado para la produccion de etanol. - Google Patents

Organismo fermentador mejorado para la produccion de etanol.

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Publication number
MX2023002490A
MX2023002490A MX2023002490A MX2023002490A MX2023002490A MX 2023002490 A MX2023002490 A MX 2023002490A MX 2023002490 A MX2023002490 A MX 2023002490A MX 2023002490 A MX2023002490 A MX 2023002490A MX 2023002490 A MX2023002490 A MX 2023002490A
Authority
MX
Mexico
Prior art keywords
fermenting organism
saccharomyces cerevisiae
fermenting
ethanol production
mbg5248
Prior art date
Application number
MX2023002490A
Other languages
English (en)
Inventor
Philip John Livingstone Bell
Maeda Roberto Nobuyuki
Original Assignee
Novozymes As
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Publication date
Application filed by Novozymes As filed Critical Novozymes As
Publication of MX2023002490A publication Critical patent/MX2023002490A/es

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/02Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06Ethanol, i.e. non-beverage
    • C12P7/08Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor
    • C12N1/16Yeasts; Culture media therefor
    • C12N1/18Baker's yeast; Brewer's yeast
    • C12N1/185Saccharomyces isolates
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    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/22Processes using, or culture media containing, cellulose or hydrolysates thereof
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52Genes encoding for enzymes or proenzymes
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
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    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
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    • C12N9/10Transferases (2.)
    • C12N9/12Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
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    • C12Y202/00Transferases transferring aldehyde or ketonic groups (2.2)
    • C12Y202/01Transketolases and transaldolases (2.2.1)
    • C12Y202/01001Transketolase (2.2.1.1)
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    • C12Y202/00Transferases transferring aldehyde or ketonic groups (2.2)
    • C12Y202/01Transketolases and transaldolases (2.2.1)
    • C12Y202/01002Transaldolase (2.2.1.2)
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    • C12Y207/00Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/01Phosphotransferases with an alcohol group as acceptor (2.7.1)
    • C12Y207/01017Xylulokinase (2.7.1.17)
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    • C12Y501/00Racemaces and epimerases (5.1)
    • C12Y501/03Racemaces and epimerases (5.1) acting on carbohydrates and derivatives (5.1.3)
    • C12Y501/03001Ribulose-phosphate 3-epimerase (5.1.3.1)
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    • C12Y503/00Intramolecular oxidoreductases (5.3)
    • C12Y503/01Intramolecular oxidoreductases (5.3) interconverting aldoses and ketoses (5.3.1)
    • C12Y503/01006Ribose-5-phosphate isomerase (5.3.1.6)
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    • C12Y503/00Intramolecular oxidoreductases (5.3)
    • C12Y503/02Intramolecular oxidoreductases (5.3) interconverting keto- and enol-groups (5.3.2)
    • C12Y503/020052,3-Diketo-5-methylthiopentyl-1-phosphate enolase (5.3.2.5)
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12R2001/00Microorganisms ; Processes using microorganisms
    • C12R2001/645Fungi ; Processes using fungi
    • C12R2001/85Saccharomyces
    • C12R2001/865Saccharomyces cerevisiae
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y503/00Intramolecular oxidoreductases (5.3)
    • C12Y503/01Intramolecular oxidoreductases (5.3) interconverting aldoses and ketoses (5.3.1)
    • C12Y503/01005Xylose isomerase (5.3.1.5)
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02EREDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00Technologies for the production of fuel of non-fossil origin
    • Y02E50/10Biofuels, e.g. bio-diesel

Abstract

La presente invención se refiere a procesos para producir etanol que comprenden sacarificar material que contiene celulosa o almidón y fermentar el material sacarificado con un microorganismo fermentador para producir etanol. El organismo fermentador es la cepa de Saccharomyces cerevisiae MBG5151 (depositada con el n.º de registro Y-67971 en la Agricultural Research Service Culture Collection (NRRL), Illinois 61604 EE. UU.), la cepa de Saccharomyces cerevisiae MBG5248 (depositada con el n.º de registro Y-68015 en la Agricultural Research Service Culture Collection (NRRL), Illinois 61604 EE. UU.) o un organismo fermentador que tiene propiedades que son las mismas o aproximadamente las mismas que las de Saccharomyces cerevisiae MBG5151 o MBG5248.
MX2023002490A 2020-09-04 2021-09-03 Organismo fermentador mejorado para la produccion de etanol. MX2023002490A (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202063074709P 2020-09-04 2020-09-04
US202163157551P 2021-03-05 2021-03-05
PCT/EP2021/074372 WO2022049250A1 (en) 2020-09-04 2021-09-03 Improved fermenting organism for ethanol production

Publications (1)

Publication Number Publication Date
MX2023002490A true MX2023002490A (es) 2023-03-09

Family

ID=78078173

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2023002490A MX2023002490A (es) 2020-09-04 2021-09-03 Organismo fermentador mejorado para la produccion de etanol.

Country Status (6)

Country Link
US (1) US20230332188A1 (es)
EP (1) EP4208559A1 (es)
AU (1) AU2021338555A1 (es)
CA (1) CA3191025A1 (es)
MX (1) MX2023002490A (es)
WO (1) WO2022049250A1 (es)

Family Cites Families (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK0695349T3 (da) 1993-03-10 2004-06-01 Novozymes As Enzymer med xylanaseaktivitet stammende fra Aspergillus Aculeatus
US5646025A (en) 1995-05-05 1997-07-08 Novo Nordisk A/S Scytalidium catalase gene
ES2166316B1 (es) 2000-02-24 2003-02-16 Ct Investig Energeticas Ciemat Procedimiento de produccion de etanol a partir de biomasa lignocelulosica utilizando una nueva levadura termotolerante.
AU2002316785A1 (en) 2001-05-18 2002-12-03 Novozymes A/S Polypeptides having cellobiase activity and polynucleotides encoding same
ES2319757T5 (es) 2002-01-23 2018-05-22 Dsm Ip Assets B.V. Fermentación de azúcares de pentosa
ES2437198T3 (es) 2003-10-28 2014-01-09 Novozymes Inc. Polipéptidos con actividad de beta-glucosidasa y polinucleótidos aislados que codifican los polipéptidos
EP1733033B1 (en) 2004-02-06 2012-06-20 Novozymes Inc. Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
DK2322630T3 (en) 2004-02-12 2017-02-13 Novozymes Inc Polypeptides with xylanase activity and polynucleotides encoding them
US8257959B2 (en) 2004-06-08 2012-09-04 Microbiogen Pty Ltd Non-recombinant Saccharomyces strains that grow on xylose
DK176540B1 (da) 2004-09-24 2008-07-21 Cambi Bioethanol Aps Fremgangsmåde til behandling af biomasse og organisk affald med henblik på at udvinde önskede biologisk baserede produkter
JP5804666B2 (ja) 2005-04-12 2015-11-04 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニーE.I.Du Pont De Nemours And Company バイオマスの処理および利用における別の供給流れの集中
AT501898B1 (de) 2005-05-19 2006-12-15 Paul Dipl Ing Dr Fricko Verfahren zur herstellung von getrockneten mikroorganismen
ES2538360T3 (es) 2006-07-21 2015-06-19 Novozymes, Inc. Métodos para aumentar la secreción de polipéptidos que tienen actividad biológica
ES2560805T3 (es) 2009-09-29 2016-02-22 Novozymes Inc. Polipéptidos con actividad mejoradora celulolítica y polinucleótidos que los codifican
DK2496694T3 (en) 2009-11-06 2017-06-06 Novozymes Inc COMPOSITIONS FOR SACCHARIFYING CELLULOS MATERIAL
CN103124783A (zh) 2010-06-03 2013-05-29 马斯科马公司 表达用于使用淀粉和纤维素进行联合生物加工的糖分解酶的酵母
US9057086B2 (en) 2010-08-12 2015-06-16 Novozymes, Inc. Compositions comprising a polypeptide having cellulolytic enhancing activity and a bicycle compound and uses thereof
MX2013002586A (es) 2010-10-01 2013-03-21 Novozymes Inc Variantes de beta-glucosidasa y polinucleotidos que codifican las mismas.
WO2012103288A1 (en) 2011-01-26 2012-08-02 Novozymes A/S Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
WO2012103293A1 (en) 2011-01-26 2012-08-02 Novozymes A/S Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
CN102174549B (zh) 2011-02-22 2012-10-10 山东大学 一种编码木糖异构酶的核酸分子及其编码的木糖异构酶
DK2689011T3 (en) 2011-03-25 2018-01-22 Novozymes As PROCEDURE FOR DEGRADATION OR CONVERSION OF CELLULOSE-SUBSTANCING MATERIAL
ES2809509T3 (es) 2011-05-05 2021-03-04 Procter & Gamble Composiciones y métodos que comprenden variantes de serina proteasa
BR112014002401B1 (pt) 2011-08-04 2021-08-03 Novozymes A/S Célula hospedeira transgênica de fungo filamentoso, métodos para produzir um polipeptídeo e uma proteína, e, construto de ácido nucleico ou um vetor de expressão
BR112014004190A2 (pt) 2011-08-24 2017-03-01 Novozymes Inc método para construir uma cepa fúngida filamentosa, cepa fúngida filamentosa, método para produzir múltiplos polipeptídeos recombinantes, e, construto em tandem
CN103890165A (zh) 2011-08-24 2014-06-25 诺维信股份有限公司 纤维素分解酶组合物及其用途
DK3198001T3 (da) 2014-09-23 2021-11-08 Novozymes As Fremgangsmåde til fremstilling af ethanol og fermentering af organismer
DE102014017721A1 (de) 2014-12-02 2016-06-02 Mahle International Gmbh Verfahren zum Herstellen eines verlorenen Gießkerns, Gießkern sowie unter Verwendung eines derartigen Gießkerns hergestellter Kühlkanalkolben
WO2018222990A1 (en) 2017-06-02 2018-12-06 Novozymes A/S Improved yeast for ethanol production
MX2020008309A (es) 2018-02-15 2020-10-14 Novozymes As Levadura mejorada para produccion de etanol.
CA3107110A1 (en) 2018-07-25 2020-01-30 Novozymes A/S Enzyme-expressing yeast for ethanol production

Also Published As

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US20230332188A1 (en) 2023-10-19
CA3191025A1 (en) 2022-03-10
WO2022049250A1 (en) 2022-03-10
EP4208559A1 (en) 2023-07-12
AU2021338555A1 (en) 2023-03-09
WO2022049250A8 (en) 2022-11-03

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