WO2017022490A1 - 多糖類-タンパク質複合体の製造方法 - Google Patents
多糖類-タンパク質複合体の製造方法 Download PDFInfo
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- WO2017022490A1 WO2017022490A1 PCT/JP2016/071305 JP2016071305W WO2017022490A1 WO 2017022490 A1 WO2017022490 A1 WO 2017022490A1 JP 2016071305 W JP2016071305 W JP 2016071305W WO 2017022490 A1 WO2017022490 A1 WO 2017022490A1
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/40—Effervescence-generating compositions
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12C—BEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
- C12C5/00—Other raw materials for the preparation of beer
- C12C5/02—Additives for beer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12G—WINE; PREPARATION THEREOF; ALCOHOLIC BEVERAGES; PREPARATION OF ALCOHOLIC BEVERAGES NOT PROVIDED FOR IN SUBCLASSES C12C OR C12H
- C12G3/00—Preparation of other alcoholic beverages
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/006—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
- C08B37/0063—Glycosaminoglycans or mucopolysaccharides, e.g. keratan sulfate; Derivatives thereof, e.g. fucoidan
Definitions
- the present invention relates to a method for producing a polysaccharide-protein complex.
- foaming may bring a particularly high added value to the food.
- Typical examples of such foods include sparkling beverages such as beer.
- the beer foam has important functions such as beautiful appearance, suppressing the escape of carbon dioxide gas, protecting the taste by preventing the beer from touching the air, and creating a scent by foaming. For this reason, improving foam retention is an important factor in making high-quality beer.
- This is not limited to beer, and the same applies to beer-like beverages such as beer liquor and third beer, which are alternative beverages for beer.
- a beer-like beverage with a reduced amount of malt has a problem that foam retention is inferior to beer. This is because proteins and carbohydrates derived from malt contribute to beer foam retention.
- Non-alcoholic beer-taste drinks have become widespread in recent years due to changes in social conditions and diversification of lifestyles, and the market is rapidly expanding.
- Non-alcoholic beer-taste beverages generally refer to beverages containing 1% or less of alcohol in beverages, and are classified as “soft drinks” on the Japanese food classification.
- a method of adding carbonic acid and other ingredients, a method of adding various ingredients to a malt extract that can be taken from malt without using wort, a method of seasoning beer style using soft drinks, and expressing beer flavor, etc. Can be mentioned.
- Malt, sugar, hops, malt extract, etc. are used as the main raw material of the non-alcohol beer-taste beverage.
- the amount of malt used is smaller than beer, as with sparkling liquor and third beer.
- carbonated soft drinks such as cider, cola, and juice that contain carbonic acid in the beverage cannot be expected to have foaming due to protein.
- the conventional carbonated soft drink has a refreshing feeling due to foam, it is not always preferable from the viewpoint of taste, for example, the generated foam is rough, the foam is poor, and the stimulation by carbonation is too strong. For this reason, even in carbonated soft drinks, there is a demand for a technique for generating fine bubbles and improving foam retention.
- Patent Document 1 As a method for solving the problem of foaming of malted alcoholic beverages and effervescent alcoholic beverages that do not use malt, a method of using saponin, a thickener or the like as a foaming improver in sparkling sake (Patent Document 1), peas A method of using a pea protein extracted from a foaming alcoholic beverage as a foam-improving substance (Patent Document 2), a method of using a sorghum degradation product in an effervescent beverage (Patent Document 3), and the like have been proposed. However, in all cases, the texture of the foam is rough and the foam retention effect is insufficient.
- Patent Document 4 a method using a polypeptide obtained by enzymatic degradation of protein.
- the molecular weight distribution of the enzyme degradation product is wide, and a large amount of contaminating fractions that do not contribute to foam quality improvement or foam retention is contained, so that the amount added to obtain a sufficient effect is increased. Therefore, it remains as a problem that the taste of the foaming material itself impairs the flavor of the beverage and its usage is greatly limited.
- the polypeptide obtained by enzymatic degradation is excellent in foaming power, the function of retaining the generated foam is not sufficient, and a problem remains.
- Patent Document 5 As a method for solving the problem of foaming of a carbonated soft drink not containing alcohol, a method for producing a highly foamable soft drink having a composition containing a saponin component and carbon dioxide in a raw material liquid (Patent Document 5), a saponin and an oligosaccharide A method for producing an effervescent-preferred beverage with improved foaming and foaming has been proposed (Patent Document 6).
- these techniques have a problem of bitterness due to the saponin component.
- Patent Document 7 a method of using soy dietary fiber (Patent Document 7) for generating and retaining fine bubbles and improving foam retention and mouthfeel is proposed for unfermented beer-taste beverages.
- Patent Document 7 a method of using soy dietary fiber (Patent Document 7) for generating and retaining fine bubbles and improving foam retention and mouthfeel is proposed for unfermented beer-taste beverages.
- this technology has a problem that the amount added to give a sufficient foam retention effect is large, and that the flavor unique to soybeans impairs the flavor of the beverage itself, and the usage is greatly limited. Has been.
- Patent Document 8 As a means for solving the problem of flavor in the use of soybean dietary fiber, a method using a water-soluble pea polysaccharide has been proposed (Patent Document 8). Is not enough and there is room for improvement.
- the present invention (1) A fraction having a molecular weight of 12,000 or more, comprising a heating step of heating a polysaccharide containing uronic acid as a constituent sugar at a pH of 2 to 5 and 80 ° C. or more and 180 ° C.
- a method for producing a polysaccharide-protein complex wherein the fraction having a molecular weight of 500 or more and less than 12000 is 30% by mass or more, (2) The production method according to (1), wherein the ratio of polysaccharide to protein in the polysaccharide-protein complex is 10: 1 to 1: 100 on a mass basis, (3) The production method according to (1) or (2), wherein the polysaccharide is derived from pea seeds, (4) The production method according to any one of (1) to (3), wherein the pH in the heating step is 2.5 to 3.5, (5) A method for stabilizing foam of an effervescent beverage, wherein a polysaccharide containing uronic acid as a constituent sugar is heated at a pH of 2 to 5 and at a temperature of 80 ° C.
- a polysaccharide containing uronic acid as a constituent sugar is heated in the presence of protein at a pH of 2 to 5 and 80 ° C. or higher and 180 ° C. or lower, and a fraction having a molecular weight of 12000 or higher is 30% by mass or lower and a molecular weight is 500 or higher.
- a method for producing an effervescent beverage comprising a step of obtaining a polysaccharide-protein complex having a fraction less than 12000 of 30% by mass or more, and a step of adding the polysaccharide-protein complex to the effervescent beverage material, (7)
- Polysaccharide-protein complex (9) The complex according to claim 8, wherein the ratio of polysaccharide to protein in the polysaccharide-protein complex is 10: 1 to 1: 100 on a mass basis. (10) The complex according to (8) or (9), wherein the polysaccharide is derived from pea seeds, (11) A foam stabilizer comprising the composite according to any one of (8) to (10) as an active ingredient. (12) A sparkling beverage containing the foam stabilizer according to (11), (13) The sparkling beverage according to (12), which contains alcohol, It is.
- the polysaccharide-protein complex according to the present embodiment (hereinafter, simply referred to as “complex” in some cases) is heated and contains a polysaccharide containing uronic acid as a constituent sugar and a protein. .
- “heated” means that it has undergone a heat treatment, and includes the case where it has been cooled.
- the polysaccharide used in the present embodiment only needs to contain uronic acid as a constituent sugar, and is preferably water-soluble.
- the polysaccharide is preferably a plant-derived polysaccharide obtained by extraction from a plant raw material, or a microorganism-derived polysaccharide produced by a microorganism by fermentation or the like.
- the amount of uronic acid in the polysaccharide may be, for example, 0.01 to 50% by mass.
- Examples of uronic acid include D-glucuronic acid, D-galacturonic acid, D-mannuronic acid, and L-guluronic acid.
- plant-derived polysaccharides for example, water-soluble pea polysaccharides derived from pea seeds and water-soluble soybean polysaccharides derived from soybean, which will be described in detail below, can be used. These polysaccharides are known to contain uronic acid as a constituent sugar.
- the plant-derived polysaccharide source containing uronic acid as a constituent sugar may be, for example, pectin, tragacanth gum, gum arabic, gati gum, karaya gum, psyllium seed gum, agar, alginic acids, etc. It may be.
- Pectin may be derived from, for example, citrus fruits, or may be derived from potato, beet or the like.
- alginic acid means alginic acid or its derivative (s). Examples of the microorganism-derived polysaccharide source containing uronic acid as a constituent sugar include xanthan gum and gellan gum.
- polysaccharides or polysaccharide sources may be used alone or in combination of two or more.
- the polysaccharide containing uronic acid as a constituent sugar water-soluble pea polysaccharide or water-soluble soybean polysaccharide is preferably used, and water-soluble pea polysaccharide is more preferably used.
- a polysaccharide isolated or extracted in advance may be used, or a polysaccharide source containing components other than the polysaccharide may be used.
- the water-soluble pea polysaccharide is a water-soluble polysaccharide obtained by extraction from pea seeds.
- the water-soluble pea polysaccharide may be obtained by extraction from a processed pea seed such as a fiber fraction obtained by removing the protein fraction and starch fraction contained in the pea seed.
- Water can be used as a solvent for extraction of water-soluble pea polysaccharides from pea seeds.
- the water may be hot water.
- the pH at the time of extraction is preferably 3-12. When the pH is 3 or more, hydrolysis of the polysaccharide can be suppressed, and when the pH is 12 or less, the elimination and decomposition of the polysaccharide can be suppressed.
- the pH during extraction is more preferably 4-10.
- the water-soluble pea polysaccharide is, for example, adjusted to pH 3 to 12 after adding 5 to 20 times the amount of water to pea seed as a raw material or a processed product thereof, and is 60 ° C. or higher and 180 ° C. or lower, preferably 60 ° C.
- the extraction can be performed at a temperature of 150 ° C. or lower or 80 ° C. or higher and 180 ° C. or lower, more preferably 80 ° C. or higher and 150 ° C. or lower, and still more preferably 80 ° C. or higher and 130 ° C. or lower.
- the extraction time can be, for example, 0.5 to 3 hours, and can be arbitrarily adjusted depending on the state of the raw material, temperature, and the like.
- the obtained extract can be used as a water-soluble pea polysaccharide source after the insoluble fiber content in the liquid is separated by a centrifuge, etc., and further, protein removal, starch removal, desalting, pigment component
- a water-soluble pea polysaccharide source a product that has been subjected to refining treatment such as removal, high temperature sterilization, retort sterilization, electromagnetic wave sterilization, high temperature vacuum sterilization, ozone sterilization, electrolytic water sterilization, indirect heat sterilization, etc. Good.
- Starch can be removed by, for example, decomposition with amylase.
- the water-soluble pea polysaccharide source may be used in a liquid state or may be used after being dried by freeze drying, spray drying or the like.
- the water-soluble soybean polysaccharide is a water-soluble polysaccharide obtained by extraction from soybean.
- the water-soluble soybean polysaccharide is preferably derived from soybean cotyledons.
- okara produced as a by-product when producing tofu, isolated soybean protein, and the like can be used.
- Okara is preferably an okara obtained from defatted soybean, and is preferably an okara produced as a by-product in the process of producing isolated soybean protein.
- the water-soluble soybean polysaccharide is prepared by adding 5 to 20 times the amount of water to raw materials such as okara and then adjusting the pH to 3 to 7 to 60 to 180 ° C., preferably 60 to 150 ° C. Extraction is preferably performed at a temperature of 80 ° C. or higher and 130 ° C. or lower, more preferably 100 ° C. or higher and 130 ° C. or lower. When extraction temperature is the said range, extraction efficiency can be improved and hydrolysis of water-soluble soybean polysaccharide can be suppressed. For the extraction time, separation of insoluble fibers in the resulting extract, purification treatment, sterilization treatment and drying, the same mode as extraction of water-soluble pea polysaccharide can be applied.
- the protein used in the production of the polysaccharide-protein complex according to the present embodiment may be any protein species, for example, soy protein, pea protein, egg white albumin, wheat gluten, casein, oat protein, mung bean protein. , Peanut protein, lentil protein and the like.
- a protein may be used individually by 1 type and may use 2 or more types together.
- the protein is preferably soy protein, casein, pea protein or egg white in that the effect of foam stability of the sparkling beverage can be enhanced.
- the polysaccharide-protein complex according to the present embodiment can be obtained by complexing the polysaccharide and the protein by heat-treating the polysaccharide in the presence of the protein.
- Conjugation involves simultaneously performing a heat extraction step of polysaccharide from a polysaccharide source and a heating step for conjugation of the polysaccharide and protein, that is, extraction of the polysaccharide from the polysaccharide source.
- Complexing is preferably performed in the presence of water, more preferably in an aqueous solution.
- concentration of the polysaccharide in the aqueous solution during heating is preferably 0.1 to 10% by mass, and preferably 1 to 5% by mass.
- the protein concentration in the aqueous solution during heating is preferably 0.1 to 10% by mass, and preferably 1 to 5% by mass.
- the polysaccharide and protein can be complexed by, for example, the following method.
- the polysaccharide and protein are suspended in an aqueous system, and the suspension is adjusted to pH 2 to 5, preferably pH 2.5 to 3.5.
- Acids that can be used in the food industry such as hydrochloric acid, phosphoric acid, sulfuric acid, lactic acid, citric acid, acetic acid, and oxalic acid, can be used without particular limitation.
- heat treatment is performed at a temperature of 80 ° C. or higher and 180 ° C. or lower. Thereby, the polysaccharide and the protein are complexed, and a liquid containing the polysaccharide-protein complex can be obtained.
- the heating temperature is preferably 100 to 160 ° C, more preferably 110 to 140 ° C.
- the heating temperature is 80 ° C. or higher, the complexing reaction can be efficiently performed in a shorter time. Moreover, excessive decomposition
- the heating time is approximately 1 to 180 minutes, but can be arbitrarily adjusted depending on the state of the raw material, temperature, and the like.
- the solid content in the suspension may be separated and removed by a conventional method such as filtration or centrifugation. Even if the solid content is not removed, there is no effect on the foam stability effect of the beverage. However, if the solid content is separated and removed, precipitation is prevented from occurring in the sparkling beverage and the appearance is improved. More preferable.
- the starch in the suspension may be decomposed or removed. Starch degradation can be performed using amylase. The removal of starch can be performed, for example, by removing a precipitate generated by agglomeration by cooling or addition of an emulsifier.
- the resulting liquid containing the polysaccharide-protein complex may be subjected to purification treatment such as neutralization / desalting, removal of hydrophobic substances or low molecular substances, as necessary.
- purification methods activated carbon treatment, resin adsorption treatment, reprecipitation method using a polar solvent such as methanol, ethanol, isopropanol, and acetone, ultrafiltration method, reverse osmosis method, gel filtration method, dialysis method, ion exchange Examples thereof include a resin method, an electrodialysis method, and an ion exchange membrane method, and one of these methods may be used alone, or two or more thereof may be used in combination.
- a reprecipitation method an ultrafiltration method, a reverse osmosis method, a gel filtration method, or a dialysis method using a polar solvent because various low molecules can be removed.
- a desalting purification process it is preferable to process so that the ash in the liquid after a process may be 10 mass% or less.
- the obtained liquid containing the polysaccharide-protein complex may be used as it is for the production of an effervescent beverage or may be used after drying by a conventional method.
- the ratio of polysaccharide to protein in the polysaccharide-protein complex is preferably 10: 1 to 1: 100 on a mass basis. When the ratio is within the above range, the effect of generating and maintaining fine bubbles can be enhanced.
- the ratio of polysaccharide to protein is more preferably 5: 1 to 1:10, and even more preferably 3: 1 to 1: 4 on a mass basis.
- the polysaccharide source and the amount of protein should be adjusted so that the ratio of polysaccharide to protein in the resulting complex is within the above range. Good.
- the molecular size of the complex is important. That is, a fraction having a molecular weight of 12,000 or more in the complex is 30% by mass or less, and a fraction having a molecular weight of 500 to less than 12000 is 30% by mass or more, more preferably a fraction having a molecular weight of 12000 or more is 25% by mass or less.
- the fraction having a molecular weight of 500 or more and less than 12000 is 40% by mass or more.
- the fraction having a molecular weight of 500 or more and less than 12000 in the complex may be 100% by mass, 80% by mass or less, 60% by mass or less, or 50% by mass or less.
- the fraction having a molecular weight of 12,000 or more in the complex may be 0% by mass, 5% by mass or more, 10% by mass or more, 15% by mass or more, or 20% by mass or more.
- the fraction having a molecular weight of less than 500 in the composite is 0% by mass or more and less than 70% by mass, preferably 0% by mass or more and less than 60% by mass, and more preferably 0% by mass or more and less than 40% by mass. preferable.
- the fraction having a molecular weight of less than 500 may be 20% by mass or more and less than 40% by mass, or 30% by mass or more and less than 40% by mass.
- the molecular weight distribution of the complex can be measured using a gel filtration HPLC method. More specifically, for example, the complex is dissolved in 20 mM phosphate buffer to form an aqueous solution, and the sample filtered through a 0.2 ⁇ m filter is passed through a gel filtration chromatography column and eluted using the phosphate buffer. The elution time of the complex is determined by measuring the absorbance at 214 nm.
- a molecular weight calibration curve is prepared using, for example, cytochrome C (molecular weight 12384), aprotinin (molecular weight 6512), gastrin I (molecular weight 2098), angiotensin II (molecular weight 1046), triglutamic acid (molecular weight 405), Based on the calibration curve, the molecular weight distribution of the complex can be determined.
- the present invention also provides a foam stabilizer comprising a polysaccharide-protein complex as an active ingredient.
- the foam stabilizer may be composed of only a polysaccharide-protein complex, and may further contain an emulsifier such as monoglyceride and other various substances having a foam stabilizing effect.
- the content of the polysaccharide-protein complex in the foam stabilizer may be 10 to 100% by mass, preferably 50 to 98% by mass, and more preferably 90 to 95% by mass.
- the foam of the sparkling beverage can be stabilized by adding the polysaccharide-protein complex according to the present embodiment to the sparkling beverage raw material. More specifically, by adding the polysaccharide-protein complex according to the present embodiment to the sparkling beverage raw material, it is possible to easily generate a lot of fine bubbles in the sparkling beverage obtained, It can be held for a longer time.
- the sparkling beverage is (1) Malt alcoholic beverage (beer, happoshu, beer or happoshu mixed with another alcoholic beverage, etc.), (2) Effervescent non-alcoholic beverages containing malt components (non-alcoholic beer, etc.) (3) Effervescent alcoholic beverages that do not use malt (beer-like beverages that do not use malt classified as “other brewed liquors (effervescent) (1)” under the liquor tax law, so-called “boiled high” shochu highballs Liqueurs, etc.), (4) Carbonated soft drinks (cider, ramune, nutrition drink, non-alcoholic cocktail, etc.) that do not contain protein components that contribute to foam stability are included.
- beverages that are not normally effervescent such as tea, tea, coffee, liqueur, wine, shochu, spirits, whiskey, etc.
- the sparkling beverage includes carbonated beverages such as cider and cream soda, which are instant-type drinks in which powder is dissolved in water or hot water.
- the content of the polysaccharide-protein complex or foam stabilizer according to this embodiment in the beverage can be, for example, 0.001 to 5% by mass as the polysaccharide-protein complex. 01 to 2% by mass. By setting it as 0.001 mass% or more, a higher foam stabilization effect can be acquired.
- the polysaccharide-protein complex or foam stabilizer according to this embodiment exhibits a high foam stabilizing effect even with a small addition amount.
- the method for producing an effervescent beverage according to the present embodiment is not particularly limited to a conventionally known method except that it includes a step of adding the polysaccharide-protein complex or the foam stabilizer according to the present embodiment to the effervescent beverage material. It can be used, and the addition method and timing are not particularly limited. Even in an effervescent alcoholic beverage or a malt alcoholic beverage that does not use malt, the polysaccharide-protein complex or foam stabilizer according to the present embodiment can be added at any stage of a conventionally known production process.
- an effervescent alcoholic beverage that does not use malt is produced by producing a pre-fermentation solution containing a carbon source, a nitrogen source, hops, and water, and fermenting the pre-fermentation solution with yeast.
- a composite or foam stabilizer as part of the raw material, its foam stability is improved.
- the manufacturing process of a general malt alcoholic beverage is a fermentation process in which raw materials containing malt and water for charging are stirred and mixed, heated and saccharified, and wort is collected, and fermented by adding yeast.
- a process, a liquor storage process for storing the fermentation end liquid, and a filtration / filling process for filtering the liquor end liquid and filling the container, and the composite or foam according to the present embodiment at any stage of this production process Stabilizers can be added.
- ⁇ Comparative Example 1 60 parts of the processed pea seed product were dispersed in 940 parts of water, adjusted to pH 6 with hydrochloric acid, and then heated at 120 ° C. for 90 minutes to extract polysaccharides. The insoluble fiber in the liquid was removed by centrifugation (5,000 rpm, 30 minutes), and the supernatant was collected. After the supernatant was heated to 60 ° C., amylase (Fungamyl 800L: Novozyme) corresponding to 0.1% by mass of solid content was added at pH 6 to decompose starch for 1 hour. After heating in boiling water for 15 minutes to inactivate amylase, it was freeze-dried to obtain powdered water-soluble pea polysaccharide A.
- amylase Fungamyl 800L: Novozyme
- ⁇ Comparative Example 2 60 parts of the processed pea seed product were dispersed in 940 parts of water, adjusted to pH 3 with hydrochloric acid, and then heated at 120 ° C. for 90 minutes to extract polysaccharides. The insoluble fiber in the liquid was removed by centrifugation (5,000 rpm, 30 minutes), and the supernatant was collected. After adjusting the supernatant to pH 6 with alkali, amylase (Fungamyl 800L: Novozyme) corresponding to 0.1% by mass of solid content was added at 60 ° C. to decompose starch for 1 hour. The obtained solution was heated in boiling water for 15 minutes to inactivate amylase, and then freeze-dried to obtain powdered water-soluble pea polysaccharide B.
- amylase Fungamyl 800L: Novozyme
- Example 1 40 parts of the processed pea seeds and 20 parts of soybean protein (Fujipro-R: Fuji Oil Co., Ltd.) are dispersed in 940 parts of water, adjusted to pH 3 with hydrochloric acid, and then heated at 120 ° C. for 90 minutes. Polysaccharide extraction and polysaccharide-protein complexation were performed. The insoluble fiber in the liquid was removed by centrifugation (5,000 rpm, 30 minutes), and the supernatant was recovered. After adjusting the supernatant to pH 6 with an aqueous sodium hydroxide solution, 0.1% by mass of the solid content Amylase (Fungamyl 800L: manufactured by Novozyme) was added to decompose starch for 1 hour. The resulting solution was heated in boiling water for 15 minutes to inactivate amylase and then freeze-dried to obtain a powdered polysaccharide-protein complex A.
- soybean protein Fuji Oil Co., Ltd.
- Examples 2 to 5> instead of using soy protein, potassium casein (Tatua 500: manufactured by Tatua), wheat protein (Hokkaido Food Co., Ltd.), pea protein (Neutralis F85M: manufactured by Rocket Japan) or dried egg white (Meringue powder: manufactured by Taisei Co., Ltd.)
- soy protein potassium casein (Tatua 500: manufactured by Tatua), wheat protein (Hokkaido Food Co., Ltd.), pea protein (Neutralis F85M: manufactured by Rocket Japan) or dried egg white (Meringue powder: manufactured by Taisei Co., Ltd.)
- the polysaccharide-protein complexes B, C, D, and E were obtained in the same manner as in Example 1 except that was used.
- Example 6 30 parts of water-soluble pea polysaccharide A and 30 parts of soy protein (Fujipro-R: Fuji Oil Co., Ltd.) prepared in Comparative Example 1 were dissolved or dispersed in water and adjusted to pH 3 with hydrochloric acid, then 120 The mixture was heated at 60 ° C. for 60 minutes to complex the polysaccharide and protein. The produced insoluble matter is removed by centrifugation (5,000 rpm, 30 minutes), and the supernatant is recovered. The supernatant is adjusted to pH 4.5 with alkali, and then freeze-dried to obtain a powdered polysaccharide- Protein complex F was obtained.
- soy protein Fuji Oil Co., Ltd.
- ⁇ Comparative Example 3 30 parts of water-soluble pea polysaccharide A prepared in Comparative Example 1 and 30 parts of soy protein (Fujipro-R: Fuji Oil Co., Ltd.) were dissolved or dispersed in water, adjusted to pH 3 with hydrochloric acid, While heating, insolubles are removed by centrifugation (5,000 rpm, 30 minutes), and the supernatant is recovered. The supernatant is adjusted to pH 4.5 with alkali, and then freeze-dried to obtain a powdery powder. Composition A containing saccharides and protein was obtained.
- ⁇ Comparative Example 4 30 parts of soy protein (Fujipro-R: manufactured by Fuji Oil Co., Ltd.) is suspended in 540 parts of water and adjusted to pH 7, and then protease (protease A “Amano” SD: manufactured by Amano Enzyme) is added to the protein. .4% was added and reacted at 40 ° C. for 60 minutes. The produced insoluble matter is removed by centrifugation (5,000 rpm, 30 minutes), the supernatant is recovered, boiled at 100 ° C. for 15 minutes to inactivate the enzyme, and then freeze-dried to produce a protein enzyme degradation product. Got. Furthermore, this protein enzyme degradation product and the water-soluble pea polysaccharide B prepared in Comparative Example 2 were mixed at a ratio of 1: 2 in the form of a powder to obtain a composition B containing polysaccharide and protein.
- composition C containing a polysaccharide and a protein was prepared in the same manner as in Comparative Example 4 except that the protease used was Samoaase PC10F (Amano Enzyme) or Papain W-40 (Amano Enzyme). , D was obtained.
- a calibration curve was prepared using cytochrome C (molecular weight 12384), aprotinin (molecular weight 6512), gastrin I (molecular weight 2098), angiotensin II (molecular weight 1046) and triglutamic acid (molecular weight 405) as molecular weight markers, The molecular weight distribution was determined based on the calibration curve. The results are shown in Table 1.
- Polysaccharides, composites or compositions were dissolved in water to prepare 0.2% aqueous solutions, respectively.
- 30 ml of each of these aqueous solutions was put into a 100 ml capacity Nessler color difference tube with a stopper, stoppered so that the liquid did not leak, and shaken at a rotational speed of 150 rpm for 1 minute in the tube height direction.
- the color difference tube was fixed to the color difference tube stand and allowed to stand, and the thickness of the foam layer after 5 minutes and 60 minutes after standing was measured. The thickness of the foam layer after 60 minutes was used as an index of foam stability.
- the complex A obtained by using a processed product of pea seeds and protein as a starting material and the complex F using an existing water-soluble pea polysaccharide and protein as starting materials have substantially the same foam stability. It was shown that a composite having excellent foam stability can be produced by any of the production methods.
- the foam stability was not improved as compared with the case where the polysaccharide was used alone. It was shown that heating is important to obtain a composite with high foam stability.
- Foam quality was evaluated by visual observation and texture during tasting, and flavor was evaluated by tasting.
- Foam quality was evaluated by visual observation and texture during tasting, and flavor was evaluated by tasting.
- Foam quality was evaluated by visual observation and texture during tasting, and flavor was evaluated by tasting.
- Foam quality was evaluated by visual observation and texture during tasting, and flavor was evaluated by tasting.
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Abstract
Description
(1)構成糖としてウロン酸を含む多糖類を、タンパク質の存在下において、pH2~5かつ80℃以上180℃以下で加熱する加熱工程を含む、分子量12000以上の画分が30質量%以下、かつ分子量500以上12000未満の画分が30質量%以上である多糖類-タンパク質複合体の製造方法、
(2)上記多糖類-タンパク質複合体における、多糖類とタンパク質との比率が質量基準で10:1~1:100である、(1)に記載の製造方法、
(3)上記多糖類がエンドウ種子由来である、(1)又は(2)に記載の製造方法、
(4)上記加熱工程におけるpHが2.5~3.5である、(1)~(3)のいずれか1つに記載の製造方法、
(5)発泡性飲料の泡を安定化させる方法であって、構成糖としてウロン酸を含む多糖類を、タンパク質の存在下において、pH2~5かつ80℃以上180℃以下で加熱し、分子量12000以上の画分が30質量%以下、かつ分子量500以上12000未満の画
分が30質量%以上である多糖類-タンパク質複合体を得る工程と、上記多糖類-タンパ
ク質複合体を発泡性飲料原料に添加する工程とを含む、方法、
(6)構成糖としてウロン酸を含む多糖類を、タンパク質の存在下において、pH2~5かつ80℃以上180℃以下で加熱し、分子量12000以上の画分が30質量%以下、かつ分子量500以上12000未満の画分が30質量%以上である多糖類-タンパク質複合体を得る工程と、上記多糖類-タンパク質複合体を発泡性飲料原料に添加する工程とを含む、発泡性飲料の製造方法、
(7)上記発泡性飲料がアルコールを含有する、(6)に記載の製造方法、
(8)構成糖としてウロン酸を含む多糖類と、タンパク質とを含有し、分子量12000以上の画分が30質量%以下、かつ分子量500以上12000未満の画分が30質量%以上である、加熱された多糖類-タンパク質複合体、
(9)上記多糖類-タンパク質複合体中の多糖類とタンパク質との比率が質量基準で10:1~1:100である、請求項8に記載の複合体、
(10)上記多糖類がエンドウ種子由来である、(8)又は(9)に記載の複合体、
(11)(8)~(10)のいずれか一項に記載の複合体を有効成分とする、泡安定剤。
(12)(11)に記載の泡安定剤を含む、発泡性飲料、
(13)アルコールを含有する、(12)に記載の発泡性飲料、
である。
水分解を抑制することができる。抽出時間は、例えば0.5~3時間とすることができ、原料の状態、温度等により、任意に調整することができる。
量線に基づいて複合体の分子量分布を求めることができる。
(1)麦芽アルコール飲料(ビール、発泡酒、ビール又は発泡酒に別のアルコール飲料を混ぜたもの等)、
(2)麦芽成分を含む発泡性ノンアルコール飲料(ノンアルコールビール等)
(3)麦芽を使用しない発泡性アルコール飲料(酒税法上「その他の醸造酒(発泡性)(1)」に分類される麦芽を使用しないビール様飲料、いわゆる「酎ハイ」と呼ばれる焼酎ハイボール等のリキュール類)、
(4)泡安定性に寄与するタンパク質成分を含まない炭酸清涼飲料(サイダー、ラムネ、栄養ドリンク、ノンアルコールカクテル等)が含まれる。
なお、茶、紅茶、コーヒー、リキュール、ワイン、焼酎、スピリッツ、ウイスキー等の通常は発泡性でない飲料であっても、炭酸ガスを含有させ発泡性を持たせた場合にはこれらも発泡性飲料に含まれる。また、サイダー、クリームソーダ等の炭酸飲料で、粉末を水又は湯に溶いて飲むインスタントタイプのものも発泡性飲料に含まれる。
上記エンドウ種子処理物60部を940部の水に分散し、塩酸を用いてpH6に調整した後、120℃にて90分間加熱して多糖類を抽出した。液中の不溶性繊維を遠心分離(5,000rpm、30分間)にて除去して上清を回収した。上清を60℃に加温した後、pH6にて固形分の0.1質量%に相当するアミラーゼ(Fungamyl800L:ノボザイム社製)を添加して1時間澱粉を分解した。沸騰水中で15分間加熱しアミラーゼを失活させた後、凍結乾燥して粉末状の水溶性エンドウ多糖類Aを得た。
上記エンドウ種子処理物60部を940部の水に分散し、塩酸を用いてpH3に調整した後、120℃にて90分間加熱して多糖類を抽出した。液中の不溶性繊維を遠心分離(5,000rpm、30分間)にて除去して上清を回収した。上清をアルカリにてpH6に調整した後、60℃で、固形分の0.1質量%に相当するアミラーゼ(Fungamyl800L:ノボザイム社製)を添加して1時間澱粉を分解した。得られた溶液を沸騰水中で15分間加熱しアミラーゼを失活させた後、凍結乾燥して粉末状の水溶性エンドウ多糖類Bを得た。
上記エンドウ種子処理物40部及び大豆タンパク質(フジプロ-R:不二製油社製)20部を940部の水に分散し、塩酸を用いてpH3に調整した後、120℃にて90分間加熱し、多糖類の抽出及び多糖類とタンパク質との複合化を行った。液中の不溶性繊維を遠心分離(5,000rpm、30分間)にて除去して上清を回収し、水酸化ナトリウム水溶液にて上清をpH6に調整した後、固形分の0.1質量%に相当するアミラーゼ(Fungamyl800L:ノボザイム社製)を添加して1時間澱粉を分解した。得られた溶液を沸騰水中で15分間加熱しアミラーゼを失活させた後、凍結乾燥して粉末状の多糖類-タンパク質複合体Aを得た。
大豆タンパク質を使用する代わりに、カゼインカリウム(Tatua500:Tatua社製)、小麦タンパク質(北国フード社製)、エンドウタンパク質(ニュートラリスF85M:ロケットジャパン社製)又は乾燥卵白(メレンゲパウダー:タイセイ社製)を使用した以外は、実施例1と同様の方法で、それぞれ多糖類-タンパク質複合体B、C、D、Eを得た。
比較例1にて調製した水溶性エンドウ多糖類A 30部及び大豆タンパク質(フジプロ-R:不二製油社製)30部を水に溶解又は分散し、塩酸を用いてpH3に調整した後、120℃にて60分間加熱し、多糖類とタンパク質との複合化を行った。生成した不溶物を遠心分離(5,000rpm、30分間)にて除去して上清を回収し、アルカリにて上清をpH4.5に調整した後、凍結乾燥して粉末状の多糖類-タンパク質複合体Fを得た。
比較例1にて調製した水溶性エンドウ多糖類A 30部と大豆タンパク質(フジプロ-R:不二製油社製)30部を水に溶解又は分散し、塩酸を用いてpH3に調整した後、未加熱のまま、不溶物を遠心分離(5,000rpm、30分間)にて除去して上清を回収し、アルカリにて上清をpH4.5に調整した後、凍結乾燥して粉末状の多糖類及びタンパク質を含む組成物Aを得た。
大豆タンパク質(フジプロ-R:不二製油社製)30部を水540部に懸濁させ、pH7に調整後、対タンパク質に対してプロテアーゼ(プロテアーゼA「アマノ」SD:天野エンザイム社製)を0.4%添加して40℃にて60分間反応させた。生成した不溶物を遠心分離(5,000rpm、30分間)にて除去して上清を回収し、100℃、15分煮沸して酵素を失活させた後、凍結乾燥してタンパク質酵素分解物を得た。さらに、このタンパク質酵素分解物と比較例2にて調製した水溶性エンドウ多糖類Bとを粉末状のまま1:2の割合で混合し、多糖類及びタンパク質を含む組成物Bを得た。
使用するプロテアーゼをサモアーゼPC10F(天野エンザイム社製)、又はパパインW-40(天野エンザイム社製)に変更した以外は比較例4と同様の方法でそれぞれ調製し、多糖類及びタンパク質を含む組成物C、Dを得た。
上記の複合体A~F及び組成物A~Dについて、ゲルろ過HPLC法により分子量の確認を行った。20mMリン酸緩衝液(pH7.2)を用いて調製した複合体又は組成物の水溶液を、0.2μmのフィルターを用いてろ過した。得られたろ液を、Superdex peptide7.5/300GL(GEヘルスケア社製)にロードし、上記のリン酸緩衝液を用いて0.5ml/分で溶出させた。各複合体又は組成物の検出は、214nmの吸光度を測定することにより行った。また、分子量のマーカーとして、シトクロムC(分子量12384)、アプロチニン(分子量6512)、ガストリンI(分子量2098)、アンジオテンシンII(分子量1046)及びトリグルタミン酸(分子量405)を用いて検量線を作成し、当該検量線に基づいて分子量の分布を求めた。結果を表1に示す。
水溶性エンドウ多糖類A、B、多糖類-タンパク質複合体A~F及び組成物A、Bについて、水溶液の泡安定性を以下に記載の方法によって測定した。結果を表2に示す。
水溶性エンドウ多糖類A、B、多糖類-タンパク質複合体A~F及び組成物Aについて、非アルコール系での炭酸清涼飲料を想定して泡安定性を以下に記載の方法にて測定した。結果を表3に示す。
(泡質)
○:非常にきめ細かい
△:ややきめ細かい
×:粗い
(香味)
○:非常に良い
△:良い
×:悪い
水溶性エンドウ多糖類A、B、多糖類-タンパク質複合体A~F及び組成物Aについて、アルコール含有発泡性飲料を想定して泡安定性を以下に記載の方法にて測定した。結果を表4に示す。
(泡質)
○:非常にきめ細かい
△:ややきめ細かい
×:粗い
(香味)
○:非常に良い
△:良い
×:悪い
Claims (15)
- 構成糖としてウロン酸を含む多糖類を、タンパク質の存在下において、pH2~5かつ80℃以上180℃以下で加熱する加熱工程を含む、分子量12000以上の画分が30質量%以下、かつ分子量500以上12000未満の画分が30質量%以上である多糖類-タンパク質複合体の製造方法。
- 前記多糖類-タンパク質複合体の分子量12000以上の画分が25質量%以下、かつ分子量500以上12000未満の画分が40質量%以上である、請求項1記載の製造方法。
- 前記多糖類-タンパク質複合体における、多糖類とタンパク質との比率が質量基準で10:1~1:100である、請求項1または2に記載の製造方法。
- 前記多糖類-タンパク質複合体における、多糖類とタンパク質との比率が質量基準で5:1~1:10である、請求項1または2に記載の製造方法。
- 前記多糖類がエンドウ種子由来である、請求項1~4のいずれか一項に記載の製造方法。
- 前記加熱工程におけるpHが2.5~3.5である、請求項1~5のいずれか一項に記載の製造方法。
- 発泡性飲料の泡を安定化させる方法であって、
構成糖としてウロン酸を含む多糖類を、タンパク質の存在下において、pH2~5かつ80℃以上180℃以下で加熱し、分子量12000以上の画分が30質量%以下、かつ分子量500以上12000未満の画分が30質量%以上である多糖類-タンパク質複合体を得る工程と、
前記多糖類-タンパク質複合体を発泡性飲料原料に添加する工程とを含む、方法。 - 構成糖としてウロン酸を含む多糖類を、タンパク質の存在下において、pH2~5かつ80℃以上180℃以下で加熱し、分子量12000以上の画分が30質量%以下、かつ分子量500以上12000未満の画分が30質量%以上である多糖類-タンパク質複合体を得る工程と、
前記多糖類-タンパク質複合体を発泡性飲料原料に添加する工程とを含む、発泡性飲料の製造方法。 - 前記発泡性飲料がアルコールを含有する、請求項8に記載の製造方法。
- 構成糖としてウロン酸を含む多糖類と、タンパク質とを含有し、分子量12000以上の画分が30質量%以下、かつ分子量500以上12000未満の画分が30質量%以上である、加熱された多糖類-タンパク質複合体。
- 前記多糖類-タンパク質複合体中の多糖類とタンパク質との比率が質量基準で10:1~1:100である、請求項10に記載の複合体。
- 前記多糖類がエンドウ種子由来である、請求項10又は11に記載の複合体。
- 請求項10~12のいずれか一項に記載の複合体を有効成分とする、泡安定剤。
- 請求項13に記載の泡安定剤を含む、発泡性飲料。
- アルコールを含有する、請求項14に記載の発泡性飲料。
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- 2016-07-20 WO PCT/JP2016/071305 patent/WO2017022490A1/ja active Application Filing
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JP2018161119A (ja) * | 2017-03-27 | 2018-10-18 | サッポロビール株式会社 | 苦残低減剤 |
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WO2022196519A1 (ja) * | 2021-03-18 | 2022-09-22 | 不二製油グループ本社株式会社 | エンドウ由来の原料の加熱処理物及びそれを有効成分とする起泡剤 |
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CN112956682B (zh) * | 2021-03-30 | 2023-04-28 | 江南大学 | 一种花生来源的乳液稳定剂的制备方法 |
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CN114404321B (zh) * | 2022-01-29 | 2024-01-02 | 广州栋方生物科技股份有限公司 | 一种白及多糖稳定性溶液体系及其制备方法和应用 |
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US20180213823A1 (en) | 2018-08-02 |
JP6874681B2 (ja) | 2021-05-19 |
CN107846939A (zh) | 2018-03-27 |
JPWO2017022490A1 (ja) | 2018-05-24 |
CN107846939B (zh) | 2022-08-12 |
EP3329786A1 (en) | 2018-06-06 |
EP3329786A4 (en) | 2019-03-06 |
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