WO2010019148A1 - Methods for removing a contaminant using indigenous protein displacement ion exchange membrane chromatography - Google Patents

Methods for removing a contaminant using indigenous protein displacement ion exchange membrane chromatography Download PDF

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Publication number
WO2010019148A1
WO2010019148A1 PCT/US2008/073179 US2008073179W WO2010019148A1 WO 2010019148 A1 WO2010019148 A1 WO 2010019148A1 US 2008073179 W US2008073179 W US 2008073179W WO 2010019148 A1 WO2010019148 A1 WO 2010019148A1
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WO
WIPO (PCT)
Prior art keywords
polypeptide
membrane
antibody
antibodies
protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2008/073179
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English (en)
French (fr)
Inventor
Arick Brown
Jerome Bill, Jr.
Timothy Tully
Christopher Dowd
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genentech Inc
Original Assignee
Genentech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=39899038&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=WO2010019148(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority to EP08797895.3A priority Critical patent/EP2321337B2/en
Priority to HRP20240472TT priority patent/HRP20240472T1/hr
Priority to HUE19181531A priority patent/HUE066562T2/hu
Priority to DK14189582.1T priority patent/DK2848625T3/da
Priority to ES08797895T priority patent/ES2527943T5/es
Priority to PL14189582T priority patent/PL2848625T3/pl
Priority to SI200832219T priority patent/SI3604324T1/sl
Priority to JP2011522953A priority patent/JP5666447B2/ja
Priority to HUE14189582A priority patent/HUE047316T2/hu
Priority to EP14189582.1A priority patent/EP2848625B1/en
Priority to LTEP19181531.5T priority patent/LT3604324T/lt
Priority to LTEP14189582.1T priority patent/LT2848625T/lt
Priority to RSP20191119 priority patent/RS59232B1/sr
Priority to ES14189582T priority patent/ES2749190T3/es
Priority to PCT/US2008/073179 priority patent/WO2010019148A1/en
Priority to MX2011001506A priority patent/MX2011001506A/es
Priority to ES19181531T priority patent/ES2978373T3/es
Priority to PT141895821T priority patent/PT2848625T/pt
Priority to KR1020187036563A priority patent/KR102196883B1/ko
Application filed by Genentech Inc filed Critical Genentech Inc
Priority to BRPI0823046-3A priority patent/BRPI0823046B1/pt
Priority to CA2731943A priority patent/CA2731943C/en
Priority to CN200880131059.7A priority patent/CN102149724B/zh
Priority to DK19181531.5T priority patent/DK3604324T3/da
Priority to KR1020187008461A priority patent/KR20180033311A/ko
Priority to US13/058,796 priority patent/US10927144B2/en
Priority to EP19181531.5A priority patent/EP3604324B1/en
Priority to FIEP19181531.5T priority patent/FI3604324T3/fi
Priority to SI200832092T priority patent/SI2848625T1/sl
Priority to KR1020117005824A priority patent/KR101843915B1/ko
Priority to AU2008360625A priority patent/AU2008360625C1/en
Priority to PT191815315T priority patent/PT3604324T/pt
Priority to PL19181531.5T priority patent/PL3604324T3/pl
Publication of WO2010019148A1 publication Critical patent/WO2010019148A1/en
Priority to IL210912A priority patent/IL210912B/en
Anticipated expiration legal-status Critical
Priority to HK15109226.0A priority patent/HK1210181B/en
Priority to IL262657A priority patent/IL262657B/en
Priority to CY20191101072T priority patent/CY1122221T1/el
Priority to HRP20191848TT priority patent/HRP20191848T1/hr
Priority to US17/153,826 priority patent/US20210284685A1/en
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation

Definitions

  • contaminant is a material that is different from the desired polypeptide product.
  • the contaminant includes, without limitation: host cell materials, such as Chinese Hamster Ovary Proteins (CHOP); leached protein ⁇ ; nucleic acid; a variant, fragment, aggregate, isomer or derivative of the desired polypeptide; another polypeptide; endotoxin; viral contaminant; cell culture media component (e.g., garamycin; GENT ⁇ MYCIN ⁇ ) etc.
  • host cell materials such as Chinese Hamster Ovary Proteins (CHOP); leached protein ⁇ ; nucleic acid; a variant, fragment, aggregate, isomer or derivative of the desired polypeptide; another polypeptide; endotoxin; viral contaminant; cell culture media component (e.g., garamycin; GENT ⁇ MYCIN ⁇ ) etc.
  • Multispecific antibodies have binding specificities for at least two different epitopes, where the epitopes are usually from different antigens. While such molecules normally will only bind two antigens ⁇ i.e., bispecific antibodies, BsAbs), antibodies with additional specificities such as trispecific antibodies are encompassed by this expression when used herein.
  • trispecific antibodies examples include anti-CD3/anti-CD4/anti-CD37, anti-CD3/anti- CD5/anti-CD37 and anti-CD3/anti-CD8/anti-CD37.
  • Bispecific antibodies can be prepared as full length antibodies or antibody fragments (e.g., F(ab') 2 bispecific antibodies).
  • E. coli cloning host is E. coli 294 (ATCC 31,446), although other strains such as E. coli B, E. coli Xl 776 (ATCC 31,537), and E. coli W3110 (ATCC 27,325) are suitable. These examples are illustrative rather than limiting.
  • cukaryotic microbes such as filamentous fungi or yeast are suitable cloning or expression hosts for antibody encoding vectors.
  • vertebrate cells have been greatest in vertebrate cells, and propagation of vertebrate cells in culture (tissue culture) has become a routine procedure.
  • useful mammalian host cell lines include, but arc not limited to, monkey kidney CVl cells transformed by SV40 (COS-7, ATCC CRL 1651); human embryonic kidney cells (293 or 293 cells subcloned for growth in suspension culture, Graham et ⁇ l., J. Gen Virol. 36:59 (1977)); baby hamster kidney cells (BHK, ATCC CCL 10); Chinese hamster ovary ccllsADHFR (CHO, Urlaub el ⁇ l., Proc. N ⁇ tl. Ac ⁇ d.
  • COS-7 monkey kidney CVl cells transformed by SV40
  • human embryonic kidney cells (293 or 293 cells subcloned for growth in suspension culture, Graham et ⁇ l., J. Gen Virol. 36:59 (1977)
  • baby hamster kidney cells BHK, ATCC CCL 10
  • any of these media may be supplemented as necessary with hormones and/or other growth factors (such as insulin, transferrin, or epidermal growth factor), salts (such as sodium chloride, calcium, magnesium, and phosphate), buffers (such as HEPES), nucleotides (such as adenosine and thymidine), antibiotics (such as garamycin; GENTAMYCIN®), trace elements (defined as inorganic compounds usually present at final concentrations in the micromolar range), and glucose or an equivalent energy source. Any other necessary supplements may also be included at appropriate concentrations that would be known to those skilled in the art.
  • the culture conditions such as temperature, pi I, and the like, are those previously used with the host cell selected for expression, and will be apparent to the ordinarily skilled artisan.
  • the composition to be subjected to the purification method herein is a recombinantly produced antibody, preferably an intact antibody, expressed by a Chinese Hamster Ovary (CIIO) recombinant host cell culture.
  • the composition has been subjected to at least one purification step prior to membrane ion exchange chromatography.
  • the composition contains the antibody of interest and one or more contaminants, such as Chinese Hamster Ovary Proteins (CHOP); leached protein ⁇ ; nucleic acid; a variant, fragment, aggregate or derivative of the desired antibody; another polypeptide; endotoxin; viral contaminant; cell culture media component (e g., garamycin; GHNTAMYCIN®), etc.
  • membrane chromatography is run on either a standard chromatography system or a custom chromatography system like an AKTATM Explorer (GE Healthcare) equipped with pressure gauges, sensors, and pump plus pump controllers.
  • the membrane device is installed downstream of a pressure gauge.
  • the pll and conductivity detectors are installed downstream of the membrane device.
  • the system is thoroughly flushed with water and then with equilibration buffer before the installation of the membrane.
  • the system with the membrane is flushed with equilibration buffer until the solution pll and conductivity outlet match the equilibration buffer specification (about five membrane volumes) and a stable baseline is observed.
  • the antibody purified as disclosed herein or the composition comprising the antibody and a pharmaceutically acceptable carrier is then used for various diagnostic, therapeutic or other uses known for such antibodies and compositions.
  • the antibody may be used to treat a disorder in a mammal by administering a therapeutically effective amount of the antibody to the mammal.
  • Fairfield, Connecticut which is a programmable process purification system that includes an integrated metering pump, pressure sensor, and in-line pH, conductivity, and UV sensor.
  • the Explorer system was programmed and controlled through a computer running UNICORNTM v5.10 software (GE Healthcare, Fairfield, Connecticut). Small- scale tests were also performed using a manual system consisting of a Masterflex® L/S® digital economy drive peristaltic pump (Cole Parmer, Vernon Hills, Illinois), in-line DTXTM Plus 1 ' NF-R pressure sensor (Becton Dickinson, Franklin Lakes, New Jersey), and a AND EK- 120Oi balance ( ⁇ &D Company, Ltd., Tokyo, Japan). The balance was used to physically monitor the flow rate of the pump by measuring mass accumulation.

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Engineering & Computer Science (AREA)
  • Water Supply & Treatment (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
PCT/US2008/073179 2008-08-14 2008-08-14 Methods for removing a contaminant using indigenous protein displacement ion exchange membrane chromatography Ceased WO2010019148A1 (en)

Priority Applications (38)

Application Number Priority Date Filing Date Title
PL19181531.5T PL3604324T3 (pl) 2008-08-14 2008-08-14 Sposoby usuwania zanieczyszczeń za pomocą chromatografii membranowej jonowymiennej z zastąpieniem macierzystego białka
BRPI0823046-3A BRPI0823046B1 (pt) 2008-08-14 2008-08-14 Método para purificar um anticorpo monoclonal de uma composição compreendendo o anticorpo monoclonal e pelo menos uma proteína de ovário de hamster chinês (chop)
CN200880131059.7A CN102149724B (zh) 2008-08-14 2008-08-14 使用原地蛋白质置换离子交换膜层析清除污染物的方法
DK14189582.1T DK2848625T3 (da) 2008-08-14 2008-08-14 Fremgangsmåder til fjernelse af en kontaminant under anvendelse af ion bytningsmembrankromatografi med forskydning af naturligt forekommende proteiner
ES08797895T ES2527943T5 (es) 2008-08-14 2008-08-14 Métodos para eliminar un contaminante usando cromatografía de membrana de intercambio iónico de desplazamiento de proteínas
PL14189582T PL2848625T3 (pl) 2008-08-14 2008-08-14 Sposoby usuwania zanieczyszczeń za pomocą chromatografii membranowej jonowymiennej z zastąpieniem macierzystego białka
SI200832219T SI3604324T1 (sl) 2008-08-14 2008-08-14 Postopki za odstranjevanje kontaminata z uporabo ionskoizmenjevalne membranske kromatografije z izpodrinjenjem nativnega proteina
JP2011522953A JP5666447B2 (ja) 2008-08-14 2008-08-14 常在性タンパク質置換イオン交換メンブレンクロマトグラフィを用いた混入物除去方法
HUE14189582A HUE047316T2 (hu) 2008-08-14 2008-08-14 Eljárások szennyezés eltávolítására indigén fehérjekiszorításos ioncserés membrán kromatográfia alkalmazásával
EP14189582.1A EP2848625B1 (en) 2008-08-14 2008-08-14 Methods for removing a contaminant using indigenous protein displacement ion exchange membrane chromatography
LTEP19181531.5T LT3604324T (lt) 2008-08-14 2008-08-14 Metodai, skirti priemaišų pašalinimui panaudojant jonų mainų chromatografiją su vietiniu išstūmimu
LTEP14189582.1T LT2848625T (lt) 2008-08-14 2008-08-14 Priemaišų pašalinimo būdai, panaudojant reziduojančio baltymo pakeitimą jonų mainų membraninės chromatografijos pagalba
RSP20191119 RS59232B1 (sr) 2008-08-14 2008-08-14 Postupci za uklanjanje zagađivača koristeći hromatografiju membrane razmene jona sa razmeštanjem autohtonih proteina
ES14189582T ES2749190T3 (es) 2008-08-14 2008-08-14 Métodos para eliminar un contaminante usando cromatografía de membrana de intercambio iónico de desplazamiento de proteínas indígenas
PCT/US2008/073179 WO2010019148A1 (en) 2008-08-14 2008-08-14 Methods for removing a contaminant using indigenous protein displacement ion exchange membrane chromatography
MX2011001506A MX2011001506A (es) 2008-08-14 2008-08-14 Metodos para eliminar un contaminante utilizado cromatografia de membrana de intercambio de ion en desplazamiento de proteina indigena.
ES19181531T ES2978373T3 (es) 2008-08-14 2008-08-14 Métodos para eliminar un contaminante usando cromatografía de membrana de intercambio iónico de desplazamiento de proteínas indígenas
PT141895821T PT2848625T (pt) 2008-08-14 2008-08-14 Métodos para remover um contaminante com a utilização de cromatografia de membrana de permuta iónica de deslocação de proteína indígena.
KR1020187036563A KR102196883B1 (ko) 2008-08-14 2008-08-14 고유 단백질 대체 이온 교환 막 크로마토그래피를 이용한 오염물의 제거 방법
EP08797895.3A EP2321337B2 (en) 2008-08-14 2008-08-14 Methods for removing a contaminant using protein displacement ion exchange membrane chromatography
CA2731943A CA2731943C (en) 2008-08-14 2008-08-14 Methods for removing a contaminant using indigenous protein displacement ion exchange membrane chromatography
HRP20240472TT HRP20240472T1 (hr) 2008-08-14 2008-08-14 Postupci za eliminaciju kontaminanta uporabom kromatografije membranske ionske izmjene s premještanjem urođenog proteina
HUE19181531A HUE066562T2 (hu) 2008-08-14 2008-08-14 Eljárások szennyezés eltávolítására indigén fehérje kiszorításos ioncserés membrán kromatográfia alkalmazásával
DK19181531.5T DK3604324T3 (da) 2008-08-14 2008-08-14 Fremgangsmåder til at fjerne en kontaminant under anvendelse af ionbytningskromatografi med forskydning af iboende proteiner
KR1020187008461A KR20180033311A (ko) 2008-08-14 2008-08-14 고유 단백질 대체 이온 교환 막 크로마토그래피를 이용한 오염물의 제거 방법
US13/058,796 US10927144B2 (en) 2008-08-14 2008-08-14 Methods for removing a contaminant using indigenous protein displacement ion exchange membrane chromatography
EP19181531.5A EP3604324B1 (en) 2008-08-14 2008-08-14 Methods for removing a contaminant using indigenous protein displacement ion exchange membrane chromatography
FIEP19181531.5T FI3604324T3 (fi) 2008-08-14 2008-08-14 Menetelmiä epäpuhtauden poistamiseksi käyttäen luontaisen proteiinin syrjäytyksen ioninvaihtomembraanikromatografiaa
SI200832092T SI2848625T1 (sl) 2008-08-14 2008-08-14 Postopki za odstranjevanje kontaminanta z uporabo ionskoizmenjevalne membranske kromatografije z izpodrinjenjem nativnega proteina
KR1020117005824A KR101843915B1 (ko) 2008-08-14 2008-08-14 고유 단백질 대체 이온 교환 막 크로마토그래피를 이용한 오염물의 제거 방법
AU2008360625A AU2008360625C1 (en) 2008-08-14 2008-08-14 Methods for removing a contaminant using indigenous protein displacement ion exchange membrane chromatography
PT191815315T PT3604324T (pt) 2008-08-14 2008-08-14 Métodos para remover um contaminante com a utilização de cromatografia de membrana de permuta iónica de deslocação de proteína indígena
IL210912A IL210912B (en) 2008-08-14 2011-01-27 Methods for removing a contaminant using natural protein transfer in ion exchange membrane chromatography
HK15109226.0A HK1210181B (en) 2015-09-18 Methods for removing a contaminant using indigenous protein displacement ion exchange membrane chromatography
IL262657A IL262657B (en) 2008-08-14 2018-10-28 Methods for removing a contaminant using natural protein transfer in ion exchange membrane chromatography
CY20191101072T CY1122221T1 (el) 2008-08-14 2019-10-11 Μεθοδοι αφαιρεσης ρυπου χρησιμοποιωντας ιοντοανταλλακτικη μεμβρανικη χρωματογραφια μετατοπισης γηγενων πρωτεϊνων
HRP20191848TT HRP20191848T1 (hr) 2008-08-14 2019-10-14 Postupci odstranjivanja kontaminanta uporabom membranske kromatografije ionskom izmjenom s premještanjem autohtonog proteina
US17/153,826 US20210284685A1 (en) 2008-08-14 2021-01-20 Methods for removing a contaminant using indigenous protein displacement ion exchange membrane chromatography

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/US2008/073179 WO2010019148A1 (en) 2008-08-14 2008-08-14 Methods for removing a contaminant using indigenous protein displacement ion exchange membrane chromatography

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US13/058,796 A-371-Of-International US10927144B2 (en) 2008-08-14 2008-08-14 Methods for removing a contaminant using indigenous protein displacement ion exchange membrane chromatography
US17/153,826 Continuation US20210284685A1 (en) 2008-08-14 2021-01-20 Methods for removing a contaminant using indigenous protein displacement ion exchange membrane chromatography

Publications (1)

Publication Number Publication Date
WO2010019148A1 true WO2010019148A1 (en) 2010-02-18

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2008/073179 Ceased WO2010019148A1 (en) 2008-08-14 2008-08-14 Methods for removing a contaminant using indigenous protein displacement ion exchange membrane chromatography

Country Status (22)

Country Link
US (2) US10927144B2 (enExample)
EP (3) EP3604324B1 (enExample)
JP (1) JP5666447B2 (enExample)
KR (3) KR101843915B1 (enExample)
CN (1) CN102149724B (enExample)
AU (1) AU2008360625C1 (enExample)
BR (1) BRPI0823046B1 (enExample)
CA (1) CA2731943C (enExample)
CY (1) CY1122221T1 (enExample)
DK (2) DK2848625T3 (enExample)
ES (3) ES2749190T3 (enExample)
FI (1) FI3604324T3 (enExample)
HR (2) HRP20240472T1 (enExample)
HU (2) HUE066562T2 (enExample)
IL (2) IL210912B (enExample)
LT (2) LT2848625T (enExample)
MX (1) MX2011001506A (enExample)
PL (2) PL3604324T3 (enExample)
PT (2) PT3604324T (enExample)
RS (1) RS59232B1 (enExample)
SI (2) SI2848625T1 (enExample)
WO (1) WO2010019148A1 (enExample)

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WO2012030512A1 (en) * 2010-09-03 2012-03-08 Percivia Llc. Flow-through protein purification process
WO2013096322A1 (en) * 2011-12-22 2013-06-27 Genentech, Inc Ion exchange membrane chromatography
EP2649088A4 (en) * 2010-12-10 2014-09-03 Tracy Thompson COMPOSITIONS FOR SEPARATION METHODS
WO2019246153A1 (en) * 2018-06-19 2019-12-26 Bristol-Myers Squibb Company Methods of purifying proteins using chromatography
CN110944942A (zh) * 2017-09-07 2020-03-31 雪佛龙美国公司 Afx骨架类型分子筛的合成
EP2575847B2 (en) † 2010-05-25 2022-04-27 F. Hoffmann-La Roche AG Methods of purifying polypeptides
US20220275024A1 (en) * 2019-08-15 2022-09-01 Fujifilm Diosynth Biotechnologies Uk Limited Process for Purifying Monoclocal Antibodies

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DK2848625T3 (da) 2008-08-14 2019-10-07 Genentech Inc Fremgangsmåder til fjernelse af en kontaminant under anvendelse af ion bytningsmembrankromatografi med forskydning af naturligt forekommende proteiner
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US9150645B2 (en) 2012-04-20 2015-10-06 Abbvie, Inc. Cell culture methods to reduce acidic species
WO2013158273A1 (en) 2012-04-20 2013-10-24 Abbvie Inc. Methods to modulate c-terminal lysine variant distribution
US9067990B2 (en) 2013-03-14 2015-06-30 Abbvie, Inc. Protein purification using displacement chromatography
US9512214B2 (en) 2012-09-02 2016-12-06 Abbvie, Inc. Methods to control protein heterogeneity
SG10201610955SA (en) * 2013-02-26 2017-02-27 Agency Science Tech & Res Protein purification in the presence of nonionic organic polymers and electropositive surfaces
EP2830651A4 (en) 2013-03-12 2015-09-02 Abbvie Inc HUMAN ANTIBODIES THAT BIND TNF-ALPHA AND PREPARATION METHODS
US10023608B1 (en) 2013-03-13 2018-07-17 Amgen Inc. Protein purification methods to remove impurities
US9499614B2 (en) 2013-03-14 2016-11-22 Abbvie Inc. Methods for modulating protein glycosylation profiles of recombinant protein therapeutics using monosaccharides and oligosaccharides
US9017687B1 (en) 2013-10-18 2015-04-28 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same using displacement chromatography
US10400007B2 (en) * 2013-04-16 2019-09-03 Asahi Kasei Medical Co., Ltd. Method for purifying antibody protein
EP3052640A2 (en) 2013-10-04 2016-08-10 AbbVie Inc. Use of metal ions for modulation of protein glycosylation profiles of recombinant proteins
US9085618B2 (en) 2013-10-18 2015-07-21 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same
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