Classifications

• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K8/00—Cosmetics or similar toiletry preparations
  • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
  • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
  • A61K8/68—Sphingolipids, e.g. ceramides, cerebrosides, gangliosides
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K8/00—Cosmetics or similar toiletry preparations
  • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
  • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
  • A61K8/40—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing nitrogen
  • A61K8/41—Amines
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P17/00—Drugs for dermatological disorders
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
  • A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
  • A61Q19/00—Preparations for care of the skin
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
  • A61Q19/00—Preparations for care of the skin
  • A61Q19/007—Preparations for dry skin

Definitions

• compositions comprising a combination of a free sphingoid base and a ceramide and uses thereof
• the present invention relates to the field of topical use of compositions comprising a selected combination of sphingolipids .
• the human skin forms a structural and adapted barrier to the environment. It further plays an important physiological role since it provides not only protection and thermoregulation, but also has a metabolic and sensorial function and storage capacity.
• the lipid composition of the epidermal cells within the skin changes considerably when the cells migrate to the outer surface and differentiate.
• the cells in the basal layer contain a complex lipid composition, with phospholipids as the major constituent.
• the phospholipid content is diminished while the amount of cerebrosides (glycosylceramides) , ceramides, cholesterol and cholestorol sulphate is increased as result of de novo synthesis and storage into the so- called lamellar bodies.
• the stratum corneum horny layer
• the phospholipids and cerebrosides have vanished completely.
• the most abundant lipids in this layer are ceramides, which mainly have been formed by enzymatic deglycosylation of cerebrosides .
• the barrier function of the skin mainly is provided by the stratum corneum.
• the stratum corneum consists of corneocytes embedded in an extracellular matrix of multiple bilayers of lipids.
• the intercellular lipid phase of the stratum corneum has roughly the following composition: 40% ceramides, 25% cholesterol, 10% cholesteryl sulphate and 25% free fatty acids.
• the skin is provided with both a perfect protective layer and a filter-active permeability layer.
• lipid barrier function a deranged regulation of cell growth and differentiation (e.g. hyperproliferation and/or decreased differentiation of keratinocytes, decreased desquamation of corneocytes) , an imflammatory response and/or an infectious state.
• the skin In these skin conditions, the skin generally displays a rough, red, dry, chapped and/or swollen character.
• Typical examples of such disorders are xerosis, acne vulgaris, psoriasis, atopic dermatitis, contact dermatitis, UV-induced erythema, and the like.
• Emollient creams and lotions may relieve part of the symptoms, but often only temporarily.
• Conventional antiimflammatory creams, of which corticosteroid creams form the main part are more effective for the treatment of certain disorders but continued use may reduce the effectiveness of the treatment and/or may give side reactions.
• conventional antiinflammatory as well as antimicrobial creams typically are not adapted to restore an impaired barrier function.
• Ceramides are generally applied in cosmetics because of their moisture-retaining properties (see for instance Japanese patent application J61-260008) .
• lipid mixtures should be applied for an optimal treatment of skin disorders associated with a disrupted epidermal barrier.
• Said lipid mixtures comprise lipids selected from the three major classes of naturally-occurring epidermal lipids, i.e. the classes of ceramides, cholesterol and free fatty acids.
• these formulations have to be applied together with conventionally used therapeutic agents.
• topical compositions comprising a combination of a free sphingoid base and a ceramide have a beneficial effect when applied on skin conditions associated with an impaired barrier function, and especially when applied on skin conditions further associated with a deranged regulation of cell growth and differentiation, inflammatory and/or infectious phenomena.
• compositions suitable for topical use comprising a combination of a free sphingoid base and a ceramide.
• the topical compositions of the invention can be cosmetic as well as dermatologic compositions .
• topical compositions comprising a combination of a free sphingoid base and a ceramide have a positive and beneficial effect on skin conditions associated with an impaired lipid barrier function.
• the synergistic effects of the combination of a free sphingoid base and a ceramide become even more apparent when the compositions according to the invention are used for the treatment of skin conditions wherein an impaired lipid barrier function further is associated with a deranged regulation of cell growth and differentiation, an imflammatory condition and/or an infectious state.
• Said deranged regulation of cell growth and differentiation is characterized by conditions like hyperproliferation of keratinocytes, decreased differentiation of keratinocytes and/or decreased desquamation of corneocytes.
• the present invention shows that the presence of a free sphingoid base especially improves the efficacy of the composition of the invention with regard to its antiinflammatory and/or its antimicrobial activity. It is shown that this efficacy improvement is due to, in particular, an antimicrobial and antiinflammatory activity of the free sphingoid base.
• the free sphingoid base present in the composition according to the invention has a general structure according to Formula 1 :
• R is a straight chain or branched alkyl group having 10 to 22 carbon atoms which may optionally contain one or more double bonds and/or may optionally be substituted with one or more hydroxyl groups, preferably is a straight chain alkyl group having 12 to 18 carbon atoms, more preferably is a straight chain alkyl group having 13 carbon atoms.
• the ceramide present in the composition according to the invention has a general structure according to Formula 2:
• a and R are defined as above, and
• R ' is a straight chain or branched alkyl group having 13 to 55 carbon atoms, preferably 15 to 50 carbon atoms, more preferably 17 to 44 carbon atoms; the alkyl chain may optionally be interrupted by an oxygen atom or by an internal ester group; may optionally contain one or more double bonds; and may optionally be substituted with one or more hydroxyl groups .
• the free sphingoid base which is present in the composition of the invention preferably is a sphingosine, a sphinganine or a phytosphingosin . More preferably, the free sphingoid base is a phytosphingosine obtainable by deacetylation of tetraacetylphytosphingosine obtainable by fermentation of the yeast Pichia ciferri .
• the ceramide which is present in the composition of the invention can be extracted from a natural source, for instance a mammalian source, or can be obtained via synthetic means.
• a suitable chemical synthesis method is the acylation of a free sphingoid base with a suitable fatty acid, for instance via the acylation method as disclosed in international patent application O93/20038.
• the ceramide present in the composition of the invention is a ceramide which corresponds in stereochemical configuration to a ceramide isolatable from mammalian skin.
• Ceramides as isolated from mammalian skin typically can be subdivided in six heterogeneous classes of compounds, ceramide 1, 2, 3, 4, 5, 61 and 611. In general, these ceramides consist of a free sphingoid base in amide linkage with a nonhydroxy or an a- hydroxy fatty acid, or an ⁇ -hydroxy fatty acid esterified with an additional fatty acid.
• a ceramide which corresponds in stereochemical configuration to a mammalian skin ceramide may for instance be obtained by acylation of Pichia ciferri - derived phytosphingosine.
• Examples of such ceramides are the ceramides disclosed in international patent applications O93/20038, W095/11881, 095/25716 and O96/10557.
• an individual ceramide as well as a mixture of two or more different ceramides can be applied in a topical composition.
• said mixture of two or more different ceramides may include various ceramide combinations, the choice of a specific combination depending among others on the desired application.
• a combination of two or more representatives of each ceramide class may for instance be applied, since said combination may lead to an increased ceramide solubility in the composition according to the invention.
• Individual ceramides may tend to crystallize and consequently become inert and unfunctional .
• a further option is a combination of, on the one hand, a sphinganine- and/or sphingosine-containing ceramide and, on the other hand, a phytosphingosine-containing ceramide (e.g. ceramide 1 and/or 2 and/or 4/5 with ceramide 3 and/or Ceramide 6) .
• a combination consists of two types of ceramides having a head group which differs in hydrophilicity and this may increase barrier enhancing properties of the same.
• a combination is feasible of a ceramide containing an ⁇ -hydroxy fatty acid with a ceramide containing a non-hydroxylated fatty acid (e.g.
• ceramide 1 and/or ceramide 2 and/or ceramide 3 with ceramide 4/5 and/or ceramide 6) is feasible.
• a combination of a ceramide containing a medium chain fatty acyl group of 16 to 22 carbon atoms with a ceramide containing a long chain fatty acyl groups of 22 to 32 carbon atoms is also feasible.
• composition of the invention optionally may comprise one or more additional skin lipid compounds, such as cholesterol, cholesterol esters like cholesteryl sulphate, free fatty acids like palmitic, stearic, behenic, oleic and/or linoleic acid and/or other sphingolipids like glycoceramides .
• the composition of the invention may further comprise ceramide compounds having a short-chain acyl group, said short chain acyl group optionally being ⁇ -hydroxylated (so-called short-chain ceramides) .
• a cerebroside is understood to be a glycoceramide wherein a monosaccharide, mostly glucose or galactose, is attached to the oxygen of the -CH 2 OH group of the ceramide according to Formula 2.
• oligosaccharides frequently including sialic acid, are attached to the same.
• a short chain acyl group is meant to comprise acyl groups having 2 to 14 carbon atoms.
• a preferred ceramide with a short -chain acyl group is acetylphytosphingosine . Examples of ceramides having a short-chain ⁇ -hydroxyacyl group are disclosed in international patent application W095/29151.
• a composition comprising a free sphingoid base and a ceramide may contain as the sole type of ceramide compound a glycoceramide or a short-chain ceramide.
• the ceramide compound in the composition of the invention may be a mixture of a glycoceramide and a short-chain ceramide.
• the combination of a free sphingoid base and a ceramide may advantageously be applied in combination with a conventional antiinflammatory and/or antimicrobial agent, where said conventional antiinflammatory and/or antimicrobial agent may be applied in substantially lower concentrations than typically used, because of the activity of the free sphingoid base.
• compositions according to the invention An example of a conventionally used antiinflammatory agent is a corticosteroid.
• active ingredients which may advantageously be applied in the compositions according to the invention, in combination with a free sphingoid base and a ceramide, are agents which have an effect on skin appearance.
• yeast /3-glucan may be applied in the composition according to the invention to reduce UV-induced erythema.
• Skin-peeling agents like ⁇ -hydroxyacids, urea, salicylic acid or proteases, may be applied in the composition according to the invention to improve desquamation and/or decrease roughness of the skin.
• Retinoids may be applied in the composition according to the invention to stimulate the mitotic and metabolic activity of epidermal cells.
• Vitamin C and/or E may be applied in the composition of the invention for their antioxidant activity on skin components, which favours their application as, for instance, antiageing agents.
• the free sphingoid base as well as the ceramide may be present in the composition according to the invention in a concentration of 0.001 to 10%, preferably in a concentration of 0.005 to 5%, more preferably in a concentration of 0.01- 2%, most preferably in a concentration of 0.02-1.0%.
• the ratio of free sphingoid base to ceramide in the composition according to the invention may lie within a range of 1 to 10 to 10 to 1.
• said ratio may vary from about 1 to 5 to about 5 to 1. More preferably, said ratio may vary from about 1 to 5 to about 1 to 1.
• topical preparations of the invention further include the usual components.
• the composition comprises a vehicle to enable the active ingredients to be conveyed to the skin.
• the vehicle enables proper application on skin and/or hair, to provide both a dermatological as well as a cosmetic treatment.
• the composition may comprise a solid, semi-solid or liquid cosmetically and/or physiologically acceptable vehicle. The nature of the vehicle will depend upon the method chosen for topical administration of the composition.
• Vehicles other than water can include liquid or solid emollients, solvents, humectants, thickeners, powders, surfactants, which are also sometimes designated as emulsifiers, solubilizers, propellants and other active ingredients .
• Emollients can be classified under such general chemical categories as (fatty acid) esters, fatty acids, (fatty) alcohols, polyols, (natural) waxes, natural oils, silicone oils, both volatile and non-volatile and hydrocarbons such as mineral oil, petroleum jelly, vaseline, squalens and (iso) paraffin.
• Surfactants including emulsifiers may be cationic, nonionic, anionic or amphoteric in nature. A single type of surfactant and/or combinations of surfactants may be employed.
• nonionic surfactants are alkoxylated compounds based upon fatty alcohols, fatty acids and sorbitan.
• Anionic-type surfactants may include fatty acid soaps, lauryl sulphate salts, lauryl ether sulphate salts, alkyl benzene sulphonates, alkyl acid phosphates.
• Amphoteric surfactants include materials as dialkylamine oxide and various types of betaines, such as cocoamido propyl betaine .
• Cationic surfactants comprise quaternary ammonium compounds (Quats) such as cetyl trimethyl ammonium chloride or bromide .
• Quats quaternary ammonium compounds
• a special class of surfactants are silicone surfactants, which are high molecular weight polymers of dimethyl polysiloxane with polyoxyethylene and/or polyoxypropylene side chains having a molecular weight of 10.000 to 50.000 D.
• surfactants used for the preparation of emulsions include emulsifiers comprising compounds having a
• HLB hydrophilic/lipophilic balance
• the HLB value of the emulsifier or mixture of emulsifiers varies between about 1 and 7.
• HLB value is higher than about 7.
• Specific emulsifiers comprise emulsifiers which are able to form a lamellar phase (liquid crystalline or gel phase) . Lamellar phases are formed at the oil -water interphase of an oil-in-water emulsion and directly incorporate the free sphingoid base and the ceramide. Examples of such specific emulsifiers are:
• sucrose esters (laurate/palmitate/stearate/oleate/ isostearate) 7.
• Lecithins and other Phospholipids are :
• Propellants include propane, butane, isobutane, dimethyl ether, chlorofluoroalkanes, carbon dioxide, nitrous oxide.
• Solvents include ethyl alcohol, methylene chloride, isopropanol, ethyl ethers such as ethoxyethanol and butoxyethanol , acetone, tetrahydrofuran, dimethyl formamide, DMSO, propylene glycol , butylene glycol .
• Humectants include proteins and protein hydrolysates, amino acids, sorbitol , glycerin, sorbitol, glycols preferably PEG 200-4000 and other polyols.
• Thickeners include cross linked polyacrylates , silicone gums and poly saccharide gums such as xanthan, carrageenan, gelatin, pection and locust beans gum, hyaluronic acid and carboxylic group-containing polymers
• Powders include chalk, talc, starch, kaolin, clays, silicates, carboxyvinyl polymers.
• active ingredients include: - anti-oxidants like butyl hydroxy toluene, ascorbic acid and salts, EDTA, hydroquinone , tocopherols, gallates;
• - penetration enhancers like mono- or di-esters of C2 to CIO alcohols and C8 to C18 fatty acids, propanols, urea, sugar esters, POE esters or ethers of fatty acids and/or alcohols, butan-1,4 diol, tetrahydrofuran, salicylate salts, pyrrolidones, N-alkyl-aza-cycloheptanones, oleic acid, linoleic acid;
• sunscreens blocking UV light, like PABA's, cinnamate and salicylate derivatives;
• compositions according to the invention on affected skin areas are various and are summarized as follows: a reduction of redness, dryness, roughness and/or scaling of the skin, a reduction of pruritis, a reduction of skin lesions, an improvement in healing of small wounds, a decrease of inflammatory symptoms in affected areas, a decrease of an infectious state of the skin in affected areas.
• Examples of skin conditions which benefit from topical application of a composition according to the invention are psoriasis, atopic dermatitis, irritant and allergic contact dermatitis, seborrheic and sebostatic dermatitis, photodermatitis (UV-induced erythema) , acne, ichthyosis, xerosis, aged skin.
• the skin infections which benefit from topical application of the compositions of the invention include bacterial, fungal, yeast and viral infections. For example dandruff, impetigo, Pityriasis vesicolor, Tinea corporis, Rosacea, Herpes, venereal diseases.
• cerebrosides contrary to ceramides tend to stimulate the proliferation of keratinocytes .
• ceramides with a short-chain acyl group may be advantageous. These short-chain ceramides will have the additional effect that they are cell -permeable and known to reduce proliferation, increase differentiation and increase desquamation.
• the present invention is exemplified by several formulations and by an efficacy study using test persons with different skin disorders. Furthermore, the antiimflammatory activity of a free sphingoid base is demonstrated.
• Example 1 The present invention is exemplified by several formulations and by an efficacy study using test persons with different skin disorders. Furthermore, the antiimflammatory activity of a free sphingoid base is demonstrated.
• Formulations comprising phytosphingosine and several ceramides
• the ceramides and the free sphingoid base used in these formulations are the following: Ceramide III: N-stearoyl-phytosphingosine Ceramide IIIB: N-oleoyl -phytosphingosine Ceramide VI: N-alpha-hydroxystearoyl -phytosphingosine Phytosphingosine : 2-amino-octadecane-l , 3 , 4-triol Phytoceramide I: N-stearoyloxyheptacosanoyl -phytosphingosine
• Waterless Barrier Cream I comprising Ceramide III, Ceramide VI and Phytosphingosine
• Waterless Barrier Creams II and III comprising Ceramide III, Ceramide VI and Phytosphingosine, and additionally cholesterol and stearic acid
• Liposomal formulation comprising Ceramide III, Ceramide IIIB, Ceramide VI and phytosphingosine, and additionally cholesterol and linoleic acid
• barrier cream I was applied daily by several test persons suffering from various skin disorders. The results are indicated in Table 1. It is clear that the use of a barrier cream according to the invention results in a significant improvement of the affected skin areas . Table 1
• phytosphingosine was assessed ex vivo on excised human skin explants .
• the explant was exposed to UV-B rays as a physical, proinflammatory stress .
• the antiinflammatory effect of phytosphingosine was evaluated by measurement of the amount of the cytokine IL-l ⁇ secreted in the incubation medium of the skin explants.
• test product resp. 0% (Placebo), 0.2 and 0.5% phytosphingosine (PS) in Propylene glycol : Ethanol (60:40) .
• Dexamethasone (1 ⁇ M) was used as the reference product. The products were applied before and after irradiation ( ⁇ 2 mg/cm 2 )
• IL-l ⁇ secretion was measured in the incubation medium of the skin explants, using a standard ELISA technique. Each experimental condition was performed in triplicate.

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• 1998
  • 1998-12-07 CN CN98802328A patent/CN1112916C/zh not_active Expired - Lifetime
  • 1998-12-07 DE DE69818242T patent/DE69818242T2/de not_active Expired - Lifetime
  • 1998-12-07 KR KR1019997006968A patent/KR100639531B1/ko not_active Expired - Lifetime
  • 1998-12-07 EP EP98963566A patent/EP0975325B1/en not_active Expired - Lifetime
  • 1998-12-07 JP JP53009599A patent/JP2001510487A/ja active Pending
  • 1998-12-07 WO PCT/EP1998/008121 patent/WO1999029293A1/en not_active Ceased
  • 1998-12-07 ES ES98963566T patent/ES2207023T3/es not_active Expired - Lifetime
  • 1998-12-07 BR BRPI9807124A patent/BRPI9807124B8/pt not_active IP Right Cessation
  • 1998-12-07 US US09/367,033 patent/US20030059447A1/en not_active Abandoned
• 2003
  • 2003-06-17 US US10/463,277 patent/US7597899B2/en not_active Expired - Fee Related

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