WO1988006624A3 - Clonage et expression moleculaire de genes codant des enzymes proteolytiques - Google Patents

Clonage et expression moleculaire de genes codant des enzymes proteolytiques Download PDF

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Publication number
WO1988006624A3
WO1988006624A3 PCT/NL1988/000007 NL8800007W WO8806624A3 WO 1988006624 A3 WO1988006624 A3 WO 1988006624A3 NL 8800007 W NL8800007 W NL 8800007W WO 8806624 A3 WO8806624 A3 WO 8806624A3
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WO
WIPO (PCT)
Prior art keywords
bacillus
protoplasts
expression
dna
bacillus cells
Prior art date
Application number
PCT/NL1988/000007
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English (en)
Other versions
WO1988006624A2 (fr
Inventor
Eekelen Christiaan Albertu Van
Der Laan Johannes Cornelis Van
Leonardus Johannes S Mulleners
Original Assignee
Gist Brocades Nv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to KR1019880701347A priority Critical patent/KR0127560B1/ko
Priority to BR888805647A priority patent/BR8805647A/pt
Application filed by Gist Brocades Nv filed Critical Gist Brocades Nv
Publication of WO1988006624A2 publication Critical patent/WO1988006624A2/fr
Priority to NO884423A priority patent/NO884423D0/no
Priority to FI884904A priority patent/FI105483B/fi
Priority to BG85831A priority patent/BG49049A3/xx
Priority to DK198805939A priority patent/DK175738B1/da
Priority to SU884356848A priority patent/RU2023723C1/ru
Publication of WO1988006624A3 publication Critical patent/WO1988006624A3/fr
Priority to FI925413A priority patent/FI106726B/fi
Priority to LVP-93-1390A priority patent/LV10307B/en
Priority to FI20001152A priority patent/FI119028B/fi

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Les nouveaux procédés et les nouvelles souches industrielles de Bacillus décrits servent à la production accrue de sérine protéase. Des composés de plasmide comprenant le gène de sérine protéase sont introduits dans une cellule hôte compatible, généralement constituée par un Bacillus, étant déjà à l'origine d'une surproduction de sérine protéase. Les cellules hôtes préférées sont constituées par des cellules provenant de l'espèce PB92 de Bacillus novo et le gène préféré de sérine protéase provient également de l'espèce PB92 de Bacillus. L'intégration et la conservation du plasmide dans le chromosome de la cellule hôte peuvent être obtenues par inclusion d'une source thermosensible de réplication dans le composé à base de plasmide et par culture dans des conditions sélectives.
PCT/NL1988/000007 1987-02-27 1988-02-26 Clonage et expression moleculaire de genes codant des enzymes proteolytiques WO1988006624A2 (fr)

Priority Applications (10)

Application Number Priority Date Filing Date Title
KR1019880701347A KR0127560B1 (ko) 1987-02-27 1988-02-26 단백질 분해 효소 코드화 유전자의 분자클로닝 및 발현
BR888805647A BR8805647A (pt) 1987-02-27 1988-02-26 Processo para clonagem molecular e expressao de genes que codificam para enzimas proteoliticas
NO884423A NO884423D0 (no) 1987-02-27 1988-10-05 Molekylaer kloning og ekspresjon av gener som koder for proteolytiske enzymer.
FI884904A FI105483B (fi) 1987-02-27 1988-10-24 Menetelmä kiinnostavan endogeenisen entsyymin tuotannon tehostamiseksi alkalofiilisessa Bacillus-isäntäsolussa
BG85831A BG49049A3 (en) 1987-02-27 1988-10-25 Method for clonalling and exposure of genetics coding enzyms
DK198805939A DK175738B1 (da) 1987-02-27 1988-10-26 Molekylær kloning og ekspression af gener, der koder for proteolytiske enzymer
SU884356848A RU2023723C1 (ru) 1987-02-27 1988-10-26 Способ получения сериновой протеазы, штамм щелочефильных bacillus-продуцент сериновой протеазы
FI925413A FI106726B (fi) 1987-02-27 1992-11-27 Korkea-alkalisia proteaaseja koodaavien geenien transformointi Bacillus-bakteereihin, ja menetelmässä käytettävät vektorit, DNA-sekvenssit ja koettimet
LVP-93-1390A LV10307B (en) 1987-02-27 1993-12-29 Molecular cloning and expression of genes encoding proteolytic enzymes
FI20001152A FI119028B (fi) 1987-02-27 2000-05-15 Menetelmä alkalofiilisten Bacillus-kantojen transformoimiseksi

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
NL87200358.7 1987-02-27
EP87200358 1987-02-27

Publications (2)

Publication Number Publication Date
WO1988006624A2 WO1988006624A2 (fr) 1988-09-07
WO1988006624A3 true WO1988006624A3 (fr) 1988-11-17

Family

ID=8197584

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/NL1988/000007 WO1988006624A2 (fr) 1987-02-27 1988-02-26 Clonage et expression moleculaire de genes codant des enzymes proteolytiques

Country Status (19)

Country Link
US (1) US5217878A (fr)
JP (1) JP3026567B2 (fr)
KR (1) KR0127560B1 (fr)
CN (1) CN88101681A (fr)
AT (2) ATE181110T1 (fr)
AU (1) AU620026B2 (fr)
BG (1) BG49049A3 (fr)
BR (1) BR8805647A (fr)
CA (1) CA1339100C (fr)
DE (2) DE3856331T2 (fr)
DK (2) DK175738B1 (fr)
FI (3) FI105483B (fr)
HU (1) HU213220B (fr)
LT (1) LT4000B (fr)
LV (1) LV10307B (fr)
NO (1) NO884423D0 (fr)
PT (1) PT86864B (fr)
RU (1) RU2023723C1 (fr)
WO (1) WO1988006624A2 (fr)

Families Citing this family (50)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
HU213220B (en) * 1987-02-27 1997-03-28 Gist Brocades Nv Method for enhanced production of serine protease in transformed bacillus cells and production of the transformed cells
DK6488D0 (da) * 1988-01-07 1988-01-07 Novo Industri As Enzymer
GB2214508A (en) * 1988-01-27 1989-09-06 Bayer Ag Plasmid vector for the efficient expression of foreign genes fused with newly conceived transcriptional and translational signal sequences.
US6287841B1 (en) * 1988-02-11 2001-09-11 Genencor International, Inc. High alkaline serine protease
WO1989010976A1 (fr) * 1988-05-05 1989-11-16 The Public Health Research Institute Of The City O Bacteries gram-positives deficientes en proteases et leur utilisation comme organismes hotes pour la production de produits recombinants
US5665587A (en) * 1989-06-26 1997-09-09 Novo Nordisk A/S Modified subtilisins and detergent compositions containing same
JP3046344B2 (ja) * 1990-10-24 2000-05-29 塩野義製薬株式会社 新規プロテアーゼ
US5482849A (en) * 1990-12-21 1996-01-09 Novo Nordisk A/S Subtilisin mutants
DK58391D0 (da) * 1991-04-03 1991-04-03 Novo Nordisk As Hidtil ukendte proteaser
DE69333463D1 (de) * 1992-05-18 2004-05-06 Genencor Int Alkalische Proteasen produzierende Bakterien, und Verfahren zur Herstellung dieser alkalischen Proteasen
US6440717B1 (en) * 1993-09-15 2002-08-27 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis
US6436690B1 (en) * 1993-09-15 2002-08-20 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis wherein one or more loop regions are substituted
US6599730B1 (en) * 1994-05-02 2003-07-29 Procter & Gamble Company Subtilisin 309 variants having decreased adsorption and increased hydrolysis
US5831004A (en) 1994-10-27 1998-11-03 Affymax Technologies N.V. Inhibitors of metalloproteases, pharmaceutical compositions comprising same and methods of their use
US5646044A (en) * 1995-03-02 1997-07-08 Henkel Kommanditgesellschaft Auf Aktien Expression systems for the production of target proteins in bacillus
US6455295B1 (en) * 1995-03-08 2002-09-24 The Procter & Gamble Company Subtilisin Carlsberg variants having decreased adsorption and increased hydrolysis
US6475765B1 (en) * 1995-03-09 2002-11-05 Procter & Gamble Company Subtilisin DY variants having decreased adsorption and increased hydrolysis
IL117350A0 (en) * 1995-03-09 1996-07-23 Procter & Gamble Proteinase k variants having decreased adsorption and increased hydrolysis
JP2001503269A (ja) 1996-11-04 2001-03-13 ノボ ノルディスク アクティーゼルスカブ ズブチラーゼ変異体及び、組成物
US5766871A (en) * 1996-11-27 1998-06-16 Food Industry Research And Development Institute Screening and characterization of glutaryl-7-aminocephalosporanic acid acylase
US6485957B1 (en) 1999-04-30 2002-11-26 Ortho-Mcneil Pharmaceutical, Inc. DNA encoding the human serine protease EOS
US6420157B1 (en) * 1999-04-30 2002-07-16 Ortho-Mcneil Pharmaceutical, Inc. Zymogen activation system
US6426199B1 (en) 1999-08-31 2002-07-30 Ortho-Mcneil Pharmaceutical, Inc. Dna
US6458564B1 (en) 1999-08-31 2002-10-01 Ortho-Mcneil Pharmaceutical, Inc. DNA encoding the human serine protease T
CN1312370A (zh) * 2000-03-07 2001-09-12 上海博德基因开发有限公司 一种新的多肽——人atp依赖的丝氨酸蛋白水解酶11和编码这种多肽的多核苷酸
US6777218B1 (en) * 2000-03-14 2004-08-17 Novozymes A/S Subtilase enzymes having an improved wash performance on egg stains
DE60144145D1 (de) * 2000-04-03 2011-04-14 Maxygen Inc Subtilisin-variante
AU2003228393A1 (en) * 2002-03-29 2003-10-13 Genencor International, Inc. Ehanced protein expression in bacillus
US20100129862A1 (en) * 2003-05-12 2010-05-27 Jones Brian E Novel lipolytic Enzyme lip1
WO2004101760A2 (fr) * 2003-05-12 2004-11-25 Genencor International, Inc. Une nouvelle enzyme lipolytique dite elip
US20070213518A1 (en) * 2003-05-12 2007-09-13 Jones Brian E Novel Lipolytic Enzyme Lip2
CA2539423C (fr) 2003-09-19 2011-09-06 Ics Holdings, Inc Preparation d'un produit comestible a base de pate
US7985569B2 (en) 2003-11-19 2011-07-26 Danisco Us Inc. Cellulomonas 69B4 serine protease variants
EP1694847B1 (fr) * 2003-11-19 2012-06-13 Danisco US Inc. Serine proteases, acides nucleiques codants pour les enzymes a serine et vecteurs et cellules hotes les contenant
JP2007532114A (ja) * 2004-04-09 2007-11-15 ジェネンコー・インターナショナル・インク バチルスにおけるｐｃｋＡ修飾および増強されたタンパク質発現
WO2005123915A1 (fr) * 2004-06-21 2005-12-29 Novozymes A/S Copies multiples d'au moins deux cadres de lecture ouverte maintenus stables dans la meme orientation
US7202074B2 (en) * 2004-07-22 2007-04-10 University Of Chile Protein and nucleic acid sequence encoding a KRILL-derived cold adapted trypsin-like activity enzyme
KR20090129425A (ko) 2007-03-12 2009-12-16 다니스코 유에스 인크. 개질된 프로테아제
DK2460823T3 (da) 2007-05-10 2014-05-05 Danisco Us Inc Modificeret sekretionssystem til øget ekspression af polypeptider ibakterier
ES2562919T3 (es) 2007-08-06 2016-03-09 University Of Chile Proteína y secuencia de ADN que codifica una actividad del tipo de la subtilisina adaptada al frío
AU2008348270A1 (en) 2007-12-21 2009-07-30 Danisco Us Inc. Enhanced protein production in bacillus
FI123122B (fi) 2009-02-16 2012-11-15 Optogear Oy Laitteisto lasimateriaalin valmistamiseksi
US8530218B2 (en) * 2009-04-24 2013-09-10 Danisco Us Inc. Proteases with modified pro regions
AR076941A1 (es) 2009-06-11 2011-07-20 Danisco Us Inc Cepa de bacillus para una mayor produccion de proteina
EP2751266B1 (fr) * 2011-09-22 2017-03-29 Novozymes A/S Polypeptides dotés d'une activité de protéase et polynucléotides codant pour ceux-ci
CN103013960A (zh) * 2012-12-21 2013-04-03 青岛蔚蓝生物集团有限公司 一种碱性蛋白酶及其重组表达工程菌
US10287591B2 (en) 2013-12-31 2019-05-14 Danisco Us Inc Enhanced protein expression
EP3233893B1 (fr) 2014-12-16 2021-01-20 Danisco US Inc. Expression elevee de proteines
KR20170093247A (ko) 2014-12-19 2017-08-14 다니스코 유에스 인크. 향상된 단백질 발현
US11578319B2 (en) * 2017-05-05 2023-02-14 Bioecho Life Sciences Gmbh Rapid purification of high quality nucleic acids from biological samples

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2292042A1 (fr) * 1974-11-19 1976-06-18 Gist Brocades Nv Nouvelles compositions enzymatiques, proteolytiques et composition de lavage en contenant
EP0134048A1 (fr) * 1983-07-06 1985-03-13 Gist-Brocades N.V. Clonage moléculaire et expression dans des espèces de micro-organismes industriels
EP0138075A1 (fr) * 1983-09-16 1985-04-24 Synergen Associates, Inc. Méthode pour la génération et détection de variantes d'enzymes à thermostabilité et activité élevée
WO1986001825A1 (fr) * 1984-09-21 1986-03-27 Genex Corporation Souches de bacillus ayant des niveaux reduits de protease extracellulaire
WO1987004461A1 (fr) * 1986-01-15 1987-07-30 Amgen ANALOGUES DE SUBTILISINE THERMIQUEMENT STABLE ET STABLE AU pH, ET LEUR PROCEDE DE PRODUCTION
EP0251446A2 (fr) * 1986-04-30 1988-01-07 Genencor International, Inc. Mutants de carbonyl hydrolases non humaines, séquences d'ADN et vecteurs codants pour ceux-ci et hôtes transformés par ces vecteurs
EP0108301B1 (fr) * 1982-11-01 1989-11-23 SOLVAY ENZYMES, INC. (a Delaware corporation) Procédé de clonage hétérologue d'un gène dans un microorganisme du genre bacillus
EP0130756B1 (fr) * 1983-06-24 1991-02-06 Genencor International, Inc. Carbonyl-hydrolases procaryotiques, méthodes, ADN, vecteurs et hôtes transformés pour leur production, et compositions des détergents contenant les dites hydrolases

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1234445A (fr) 1967-10-03 1971-06-03
GB1205403A (en) 1967-11-10 1970-09-16 Novo Terapeutisk Labor As Preparation of proteolytic enzyme preparations
FR2482980A1 (fr) 1980-05-20 1981-11-27 Biomerieux Sa Procede d'obtention de preparations de toxoplasmes pour le diagnostic de la toxoplasmose, et preparations ainsi obtenues
JPS6055118B2 (ja) 1982-02-08 1985-12-03 昭和電工株式会社 新規な細菌アルカリプロテア−ゼ及びその製造方法
FR2582316B1 (fr) * 1985-05-22 1988-12-02 Centre Nat Rech Scient Vecteurs d'expression de l'a-amylase dans bacillus subtilis, souches obtenues et procede de preparation d'a-amylase
DE3527913A1 (de) * 1985-08-03 1987-02-12 Henkel Kgaa Alkalische protease, verfahren zur herstellung von hybridvektoren und genetisch transformierte mikroorganismen
HU213220B (en) * 1987-02-27 1997-03-28 Gist Brocades Nv Method for enhanced production of serine protease in transformed bacillus cells and production of the transformed cells
ES2135386T3 (es) * 1987-02-27 1999-11-01 Genencor Int Transformacion de cepas de bacillus alcalofilas.

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2292042A1 (fr) * 1974-11-19 1976-06-18 Gist Brocades Nv Nouvelles compositions enzymatiques, proteolytiques et composition de lavage en contenant
EP0108301B1 (fr) * 1982-11-01 1989-11-23 SOLVAY ENZYMES, INC. (a Delaware corporation) Procédé de clonage hétérologue d'un gène dans un microorganisme du genre bacillus
EP0130756B1 (fr) * 1983-06-24 1991-02-06 Genencor International, Inc. Carbonyl-hydrolases procaryotiques, méthodes, ADN, vecteurs et hôtes transformés pour leur production, et compositions des détergents contenant les dites hydrolases
EP0134048A1 (fr) * 1983-07-06 1985-03-13 Gist-Brocades N.V. Clonage moléculaire et expression dans des espèces de micro-organismes industriels
EP0138075A1 (fr) * 1983-09-16 1985-04-24 Synergen Associates, Inc. Méthode pour la génération et détection de variantes d'enzymes à thermostabilité et activité élevée
WO1986001825A1 (fr) * 1984-09-21 1986-03-27 Genex Corporation Souches de bacillus ayant des niveaux reduits de protease extracellulaire
WO1987004461A1 (fr) * 1986-01-15 1987-07-30 Amgen ANALOGUES DE SUBTILISINE THERMIQUEMENT STABLE ET STABLE AU pH, ET LEUR PROCEDE DE PRODUCTION
EP0251446A2 (fr) * 1986-04-30 1988-01-07 Genencor International, Inc. Mutants de carbonyl hydrolases non humaines, séquences d'ADN et vecteurs codants pour ceux-ci et hôtes transformés par ces vecteurs

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
Agricultural and Biological Chemistry, vol. 47, no. 9, September 1983 (Tokyo, JP), R. Usami et al.: "Plasmids in alkalophilic Bacillus sp.", pages 2102-2102 *
Bilogical Abstracts, vol. 82, 1986, (Philadelphia, Pennsylvenia, US), J.H. Yu et al.: "Physiological properties and transformation of alkaline-tolerant bacteria", abstract no. 86427 *
Biological Abstracts/RRM, abstract no. 34060253 (Philadelphia, PA, US), C. Pak et al.: "Cloning of protease gene produced by heat-resistant alkalinic Bacillus in Escherichia coli", & Abstract Biotechnology Genetic Engineering. *
Gene, vol. 30, 1984, Elsevier Science Publishers, V.A. Corfield et al.: "A modified protoplast-regeneration protocol facilitating the detection of cloned exoenzyme genes in Bacillus subtilus" pages 17-22 *
Nature, vol. 318, 28 November 1985, P.G. Thomas et al.: "Tailoring the pH dependence of enzyme catalysis using protein engineering", pages 375-376 *
Proceedings of the National Academy of Sciences of the USA, vol. 81, no. 4, February 1984 (Washington, US),S.-L. Wong et al.: "The subtilisin E gene of Bacillus subtilis is transcribed from a J37 promoter in vivo", pages 1184-1188 *
Spec. Publ. Soc. Gen. Microbiol., vol. 17, (Microbes Extreme Environ.), 1986, K. Horikoshi: "Genetic applications of alkalophilic microorganisms" pages 297-315 *

Also Published As

Publication number Publication date
BR8805647A (pt) 1989-10-31
CA1339100C (fr) 1997-07-29
KR890700665A (ko) 1989-04-26
FI925413A (fi) 1992-11-27
DK175738B1 (da) 2005-02-07
DE3856331D1 (de) 1999-06-17
AU620026B2 (en) 1992-02-13
FI925413A0 (fi) 1992-11-27
LV10307B (en) 1995-08-20
JP3026567B2 (ja) 2000-03-27
FI119028B (fi) 2008-06-30
WO1988006624A2 (fr) 1988-09-07
LV10307A (lv) 1994-10-20
RU2023723C1 (ru) 1994-11-30
US5217878A (en) 1993-06-08
AU1398088A (en) 1988-09-26
LTIP1820A (en) 1995-08-25
PT86864B (pt) 1992-05-29
NO884423L (no) 1988-10-05
PT86864A (pt) 1988-03-01
FI105483B (fi) 2000-08-31
DK175852B1 (da) 2005-04-04
DE3856339T2 (de) 1999-11-04
KR0127560B1 (ko) 1997-12-29
NO884423D0 (no) 1988-10-05
HU213220B (en) 1997-03-28
BG49049A3 (en) 1991-07-15
FI106726B (fi) 2001-03-30
DE3856331T2 (de) 1999-10-14
DK593988D0 (da) 1988-10-26
DK593988A (da) 1988-10-26
ATE181110T1 (de) 1999-06-15
FI884904A (fi) 1988-10-24
DK200401262A (da) 2004-08-20
CN88101681A (zh) 1988-11-09
FI884904A0 (fi) 1988-10-24
FI20001152A (fi) 2000-05-15
JPH01502876A (ja) 1989-10-05
ATE180016T1 (de) 1999-05-15
DE3856339D1 (de) 1999-07-15
LT4000B (en) 1996-06-25

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