NO884423L - Molekylaer kloning og ekspresjon av gener som koder for proteolytiske enzymer. - Google Patents
Molekylaer kloning og ekspresjon av gener som koder for proteolytiske enzymer.Info
- Publication number
- NO884423L NO884423L NO884423A NO884423A NO884423L NO 884423 L NO884423 L NO 884423L NO 884423 A NO884423 A NO 884423A NO 884423 A NO884423 A NO 884423A NO 884423 L NO884423 L NO 884423L
- Authority
- NO
- Norway
- Prior art keywords
- bacillus
- protoplasts
- dna
- bacillus cells
- dna sequence
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
- C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Mycology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Nye fremgangsmåter og nye industrielle Bae 11 lus-stamme r er tllvelebragt for øket produksjon av serln protease. Plasmldkonstrtiksjoner som Inneholder serln protease- genet, blir ført Inn 1 en kompatibel vert, generelt en Baclllus som allerede overproduserer serln protease. Foretrukne vertsceller Innbefatter de fra Baclllus novo sp. PB92 og det foretrukne serln proteasegenet er også avledet fra Baclllus PB92. Integrasjon og oppretthold- else av plasmidet 1 vertscellekromosomet kan bil oppnådd ved å Innbefatte et temperaturfølsomt repllka- sjonsorlgo 1 plasmldkonstruksjonen og dyrking under selektive betingelser.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP87200358 | 1987-02-27 | ||
PCT/NL1988/000007 WO1988006624A2 (en) | 1987-02-27 | 1988-02-26 | Molecular cloning and expression of genes encoding proteolytic enzymes |
Publications (2)
Publication Number | Publication Date |
---|---|
NO884423D0 NO884423D0 (no) | 1988-10-05 |
NO884423L true NO884423L (no) | 1988-10-05 |
Family
ID=8197584
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
NO884423A NO884423L (no) | 1987-02-27 | 1988-10-05 | Molekylaer kloning og ekspresjon av gener som koder for proteolytiske enzymer. |
Country Status (19)
Country | Link |
---|---|
US (1) | US5217878A (no) |
JP (1) | JP3026567B2 (no) |
KR (1) | KR0127560B1 (no) |
CN (1) | CN88101681A (no) |
AT (2) | ATE181110T1 (no) |
AU (1) | AU620026B2 (no) |
BG (1) | BG49049A3 (no) |
BR (1) | BR8805647A (no) |
CA (1) | CA1339100C (no) |
DE (2) | DE3856339T2 (no) |
DK (2) | DK175738B1 (no) |
FI (3) | FI105483B (no) |
HU (1) | HU213220B (no) |
LT (1) | LT4000B (no) |
LV (1) | LV10307B (no) |
NO (1) | NO884423L (no) |
PT (1) | PT86864B (no) |
RU (1) | RU2023723C1 (no) |
WO (1) | WO1988006624A2 (no) |
Families Citing this family (50)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
PT86864B (pt) * | 1987-02-27 | 1992-05-29 | Gist Brocades Nv | Processo para clonagem molecular e expressao de genes que codificam para enzimas proteoliticas |
DK6488D0 (da) * | 1988-01-07 | 1988-01-07 | Novo Industri As | Enzymer |
GB2214508A (en) * | 1988-01-27 | 1989-09-06 | Bayer Ag | Plasmid vector for the efficient expression of foreign genes fused with newly conceived transcriptional and translational signal sequences. |
US6287841B1 (en) * | 1988-02-11 | 2001-09-11 | Genencor International, Inc. | High alkaline serine protease |
HUT53154A (en) * | 1988-05-05 | 1990-09-28 | New York Health Res Inst | Process for producing protease-defective gram-positive bacteria and utilizing them as host-organizmus for producing recombinant products |
US5665587A (en) * | 1989-06-26 | 1997-09-09 | Novo Nordisk A/S | Modified subtilisins and detergent compositions containing same |
JP3046344B2 (ja) * | 1990-10-24 | 2000-05-29 | 塩野義製薬株式会社 | 新規プロテアーゼ |
US5482849A (en) * | 1990-12-21 | 1996-01-09 | Novo Nordisk A/S | Subtilisin mutants |
DK58391D0 (da) * | 1991-04-03 | 1991-04-03 | Novo Nordisk As | Hidtil ukendte proteaser |
EP0571014B1 (en) * | 1992-05-18 | 2004-03-31 | Genencor International, Inc. | Bacteria producing alkaline proteases, and production of these alkaline proteases |
US6440717B1 (en) * | 1993-09-15 | 2002-08-27 | The Procter & Gamble Company | BPN′ variants having decreased adsorption and increased hydrolysis |
US6436690B1 (en) * | 1993-09-15 | 2002-08-20 | The Procter & Gamble Company | BPN′ variants having decreased adsorption and increased hydrolysis wherein one or more loop regions are substituted |
US6599730B1 (en) * | 1994-05-02 | 2003-07-29 | Procter & Gamble Company | Subtilisin 309 variants having decreased adsorption and increased hydrolysis |
US5831004A (en) * | 1994-10-27 | 1998-11-03 | Affymax Technologies N.V. | Inhibitors of metalloproteases, pharmaceutical compositions comprising same and methods of their use |
US5646044A (en) * | 1995-03-02 | 1997-07-08 | Henkel Kommanditgesellschaft Auf Aktien | Expression systems for the production of target proteins in bacillus |
US6455295B1 (en) * | 1995-03-08 | 2002-09-24 | The Procter & Gamble Company | Subtilisin Carlsberg variants having decreased adsorption and increased hydrolysis |
US6475765B1 (en) * | 1995-03-09 | 2002-11-05 | Procter & Gamble Company | Subtilisin DY variants having decreased adsorption and increased hydrolysis |
IL117350A0 (en) * | 1995-03-09 | 1996-07-23 | Procter & Gamble | Proteinase k variants having decreased adsorption and increased hydrolysis |
US6300116B1 (en) | 1996-11-04 | 2001-10-09 | Novozymes A/S | Modified protease having improved autoproteolytic stability |
US5766871A (en) * | 1996-11-27 | 1998-06-16 | Food Industry Research And Development Institute | Screening and characterization of glutaryl-7-aminocephalosporanic acid acylase |
US6485957B1 (en) | 1999-04-30 | 2002-11-26 | Ortho-Mcneil Pharmaceutical, Inc. | DNA encoding the human serine protease EOS |
US6420157B1 (en) | 1999-04-30 | 2002-07-16 | Ortho-Mcneil Pharmaceutical, Inc. | Zymogen activation system |
US6426199B1 (en) | 1999-08-31 | 2002-07-30 | Ortho-Mcneil Pharmaceutical, Inc. | Dna |
US6458564B1 (en) | 1999-08-31 | 2002-10-01 | Ortho-Mcneil Pharmaceutical, Inc. | DNA encoding the human serine protease T |
CN1312370A (zh) * | 2000-03-07 | 2001-09-12 | 上海博德基因开发有限公司 | 一种新的多肽——人atp依赖的丝氨酸蛋白水解酶11和编码这种多肽的多核苷酸 |
US6777218B1 (en) * | 2000-03-14 | 2004-08-17 | Novozymes A/S | Subtilase enzymes having an improved wash performance on egg stains |
DE60144145D1 (de) * | 2000-04-03 | 2011-04-14 | Maxygen Inc | Subtilisin-variante |
EP2339017A3 (en) | 2002-03-29 | 2011-10-12 | Genencor International, Inc. | Enhanced protein expression in bacillus |
US20070213518A1 (en) * | 2003-05-12 | 2007-09-13 | Jones Brian E | Novel Lipolytic Enzyme Lip2 |
EP1625202A4 (en) | 2003-05-12 | 2010-10-20 | Genencor Int | NEW LIPOLYTIC ENZYME LIP1 |
US7511005B2 (en) * | 2003-05-12 | 2009-03-31 | Danisco Us Inc., Genencor Division | Lipolytic enzyme elip |
EP1667530A4 (en) | 2003-09-19 | 2011-05-25 | Ics Holdings Inc | MANUFACTURE OF A COURABLE PRODUCT OF DOUGH |
US7985569B2 (en) | 2003-11-19 | 2011-07-26 | Danisco Us Inc. | Cellulomonas 69B4 serine protease variants |
CN103421760A (zh) * | 2003-11-19 | 2013-12-04 | 金克克国际有限公司 | 丝氨酸蛋白酶、编码丝氨酸酶的核酸以及包含它们的载体和宿主细胞 |
WO2006033668A2 (en) * | 2004-04-09 | 2006-03-30 | Genencor International, Inc. | Pcka modifications and enhanced protein expression in bacillus |
EP1766002B1 (en) * | 2004-06-21 | 2015-11-18 | Novozymes A/S | Stably maintained multiple copies of at least two orf in the same orientation |
US7202074B2 (en) * | 2004-07-22 | 2007-04-10 | University Of Chile | Protein and nucleic acid sequence encoding a KRILL-derived cold adapted trypsin-like activity enzyme |
CN103667323B (zh) | 2007-03-12 | 2016-02-03 | 丹尼斯科美国公司 | 经修饰的蛋白酶 |
ATE550348T1 (de) | 2007-05-10 | 2012-04-15 | Danisco Us Inc | Modifiziertes ausscheidungssystem zur erhöhung der expression von polypeptiden in bakterien |
EP2173873B1 (en) | 2007-08-06 | 2015-11-18 | University of Chile | Protein and dna sequence encoding a cold adapted subtilisin-like activity |
KR20100098652A (ko) | 2007-12-21 | 2010-09-08 | 다니스코 유에스 인크. | 바실루스 내에서의 향상된 단백질 생산 |
FI123122B (fi) | 2009-02-16 | 2012-11-15 | Optogear Oy | Laitteisto lasimateriaalin valmistamiseksi |
AR076312A1 (es) * | 2009-04-24 | 2011-06-01 | Danisco Us Inc | Proteasas con regiones pro modificadas |
AR076941A1 (es) | 2009-06-11 | 2011-07-20 | Danisco Us Inc | Cepa de bacillus para una mayor produccion de proteina |
EP2751266B1 (en) * | 2011-09-22 | 2017-03-29 | Novozymes A/S | Polypeptides having protease activity and polynucleotides encoding same |
CN103013960A (zh) * | 2012-12-21 | 2013-04-03 | 青岛蔚蓝生物集团有限公司 | 一种碱性蛋白酶及其重组表达工程菌 |
CN106068273B (zh) | 2013-12-31 | 2021-01-08 | 丹尼斯科美国公司 | 增强的蛋白质表达 |
WO2016099917A1 (en) | 2014-12-16 | 2016-06-23 | Danisco Us Inc | Enhanced protein expression |
WO2016100128A1 (en) | 2014-12-19 | 2016-06-23 | Danisco Us Inc | Enhanced protein expression |
WO2018202911A1 (en) * | 2017-05-05 | 2018-11-08 | Bioecho Life Sciences Gmbh | Rapid purification of high quality nucleic acids from biological samples |
Family Cites Families (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB1234445A (no) | 1967-10-03 | 1971-06-03 | ||
GB1205403A (en) | 1967-11-10 | 1970-09-16 | Novo Terapeutisk Labor As | Preparation of proteolytic enzyme preparations |
GB1519148A (en) * | 1974-11-19 | 1978-07-26 | Gist Brocades Nv | Compositions of matter |
FR2482980A1 (fr) | 1980-05-20 | 1981-11-27 | Biomerieux Sa | Procede d'obtention de preparations de toxoplasmes pour le diagnostic de la toxoplasmose, et preparations ainsi obtenues |
JPS6055118B2 (ja) | 1982-02-08 | 1985-12-03 | 昭和電工株式会社 | 新規な細菌アルカリプロテア−ゼ及びその製造方法 |
ATE48156T1 (de) * | 1982-11-01 | 1989-12-15 | Miles Inc | Verfahren zur heterologen klonierung eines gens in einem bacillus-mikroorganismus. |
NZ208612A (en) * | 1983-06-24 | 1991-09-25 | Genentech Inc | Method of producing "procaryotic carbonyl hydrolases" containing predetermined, site specific mutations |
NZ208806A (en) * | 1983-07-06 | 1988-07-28 | Gist Brocades Nv | Genetic engineering of industrial microorganism species: readily-transformable host used as intermediate in transfer of dna to the industrial species; plasmids |
EP0138075A1 (en) * | 1983-09-16 | 1985-04-24 | Synergen Associates, Inc. | A method for the generation and detection of enzyme variants with enhanced themostability and activity |
US4828994A (en) * | 1984-09-21 | 1989-05-09 | Genex Corporation | Bacillus strains with reduced extracellular protease levels |
FR2582316B1 (fr) * | 1985-05-22 | 1988-12-02 | Centre Nat Rech Scient | Vecteurs d'expression de l'a-amylase dans bacillus subtilis, souches obtenues et procede de preparation d'a-amylase |
DE3527913A1 (de) * | 1985-08-03 | 1987-02-12 | Henkel Kgaa | Alkalische protease, verfahren zur herstellung von hybridvektoren und genetisch transformierte mikroorganismen |
JP2599946B2 (ja) * | 1986-01-15 | 1997-04-16 | アムジェン,インコーポレイテツド | ズブチリシン類似体 |
ATE116365T1 (de) * | 1986-04-30 | 1995-01-15 | Genencor Int | Mutante einer nicht menschlichen carbonyl- hydrolase, für diese kodierende dns-sequenzen und vektoren und durch diese vektoren transformierte wirte. |
PT86864B (pt) * | 1987-02-27 | 1992-05-29 | Gist Brocades Nv | Processo para clonagem molecular e expressao de genes que codificam para enzimas proteoliticas |
EP0896062A3 (en) * | 1987-02-27 | 1999-04-07 | Genencor International, Inc. | Transformation of alkalophilic bacillus strains |
-
1988
- 1988-02-26 PT PT86864A patent/PT86864B/pt not_active IP Right Cessation
- 1988-02-26 HU HU881832A patent/HU213220B/hu not_active IP Right Cessation
- 1988-02-26 KR KR1019880701347A patent/KR0127560B1/ko not_active IP Right Cessation
- 1988-02-26 AU AU13980/88A patent/AU620026B2/en not_active Expired
- 1988-02-26 BR BR888805647A patent/BR8805647A/pt not_active IP Right Cessation
- 1988-02-26 WO PCT/NL1988/000007 patent/WO1988006624A2/en active IP Right Grant
- 1988-02-26 JP JP63502472A patent/JP3026567B2/ja not_active Expired - Lifetime
- 1988-02-27 CN CN198888101681A patent/CN88101681A/zh active Pending
- 1988-02-29 AT AT91203297T patent/ATE181110T1/de not_active IP Right Cessation
- 1988-02-29 CA CA000560126A patent/CA1339100C/en not_active Expired - Lifetime
- 1988-02-29 AT AT88200377T patent/ATE180016T1/de not_active IP Right Cessation
- 1988-02-29 DE DE3856339T patent/DE3856339T2/de not_active Expired - Lifetime
- 1988-02-29 US US07/162,184 patent/US5217878A/en not_active Expired - Lifetime
- 1988-02-29 DE DE3856331T patent/DE3856331T2/de not_active Expired - Lifetime
- 1988-10-05 NO NO884423A patent/NO884423L/no unknown
- 1988-10-24 FI FI884904A patent/FI105483B/fi active IP Right Grant
- 1988-10-25 BG BG085831A patent/BG49049A3/xx unknown
- 1988-10-26 DK DK198805939A patent/DK175738B1/da not_active IP Right Cessation
- 1988-10-26 RU SU884356848A patent/RU2023723C1/ru active
-
1992
- 1992-11-27 FI FI925413A patent/FI106726B/fi active
-
1993
- 1993-12-29 LV LVP-93-1390A patent/LV10307B/en unknown
-
1994
- 1994-01-28 LT LTIP1820A patent/LT4000B/lt not_active IP Right Cessation
-
2000
- 2000-05-15 FI FI20001152A patent/FI119028B/fi not_active IP Right Cessation
-
2004
- 2004-08-20 DK DK200401262A patent/DK175852B1/da not_active IP Right Cessation
Also Published As
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