AU2001279674A1 - New nucleotide sequences which code for the mdha gene - Google Patents
New nucleotide sequences which code for the mdha geneInfo
- Publication number
- AU2001279674A1 AU2001279674A1 AU2001279674A AU7967401A AU2001279674A1 AU 2001279674 A1 AU2001279674 A1 AU 2001279674A1 AU 2001279674 A AU2001279674 A AU 2001279674A AU 7967401 A AU7967401 A AU 7967401A AU 2001279674 A1 AU2001279674 A1 AU 2001279674A1
- Authority
- AU
- Australia
- Prior art keywords
- polynucleotide
- sequence
- malate dehydrogenase
- gene
- thr
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Abstract
A coryneform bacterium (I) in which the mdhA gene is attenuated, preferably eliminated, is new. Independent claims are also included for the following: (1) a polynucleotide (II) from coryneform bacteria, containing a polynucleotide sequence which codes for the mdhA gene, selected from: (a) a polynucleotide which is at least 70% identical to a polynucleotide which encodes a sequence (S1) of 328 amino acids fully defined in the specification; (b) a polynucleotide which encodes a polypeptide comprising a sequence which is at least 70% identical to S1; (c) complements of the above mentioned polynucleotide sequences; and (d) a polynucleotide comprising at least 15 successive nucleotides of the above mentioned polynucleotide sequences, where the polypeptide has the activity of malate dehydrogenase; (2) an isolated polypeptide (III) with malate dehydrogenase activity, which comprises the N-terminal amino acid sequence Asn-Ser-Pro-Gln-Asn-Val-Ser-Thr-Lys-Lys-Val-Thr-Val-Thr-Gly-Ala-Ala-G ly-Gln-Ile; (3) process (M1) for the preparation of L-amino acids in particular L-lysine comprising: (a) fermenting (I) which produces the desired L-amino acid and in which the mdhA gene is attenuated; (b) concentrating the desired product in the medium or in the cells of the bacteria; and (c) isolating the L-amino acid; (4) discovering (M2) RNA, DNA and cDNA in order to isolate nucleic acids, polynucleotides or genes which code for malate dehydrogenase or have high similarity to the sequence of the malate dehydrogenase gene comprising employing (II) as hybridization probes.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE10032350A DE10032350A1 (en) | 2000-07-04 | 2000-07-04 | New coryneform bacterium in which the mdhA gene is attenuated, preferably eliminated, useful for fermentative production of L-amino acids such as L-lysine |
DE10032350 | 2000-07-04 | ||
PCT/EP2001/006928 WO2002002778A1 (en) | 2000-07-04 | 2001-06-20 | Nucleotide sequences which code for the mdha gene from corynebacterium glutamicum |
Publications (1)
Publication Number | Publication Date |
---|---|
AU2001279674A1 true AU2001279674A1 (en) | 2002-01-14 |
Family
ID=7647660
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AU2001279674A Abandoned AU2001279674A1 (en) | 2000-07-04 | 2001-06-20 | New nucleotide sequences which code for the mdha gene |
Country Status (9)
Country | Link |
---|---|
US (2) | US6995002B2 (en) |
EP (1) | EP1297148B1 (en) |
KR (1) | KR100904744B1 (en) |
CN (1) | CN1440458A (en) |
AT (1) | ATE312929T1 (en) |
AU (1) | AU2001279674A1 (en) |
DE (2) | DE10032350A1 (en) |
ES (1) | ES2254459T3 (en) |
WO (1) | WO2002002778A1 (en) |
Families Citing this family (17)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN100506990C (en) * | 2004-02-24 | 2009-07-01 | 中国科学院植物研究所 | Malic dehydrogenase related to high photosynthetic capacity and resisting reversal of wheat, coded genes and method for breeding plants of resisting reversal |
KR100789270B1 (en) | 2005-11-30 | 2008-01-02 | 씨제이 주식회사 | - - A microorganism of corynebacterium genus having enhanced L-lysine productivity and a method of producing L-lysine using the same |
DE102006032634A1 (en) | 2006-07-13 | 2008-01-17 | Evonik Degussa Gmbh | Process for the preparation of L-amino acids |
KR100822041B1 (en) * | 2006-12-21 | 2008-04-15 | 씨제이제일제당 (주) | - Corynebacterium glutamicum enhanced expression of moaA gene encoding molybdenum cofactor biosynthesis enzyme A and method for producing L-lysine using the same |
US8647642B2 (en) | 2008-09-18 | 2014-02-11 | Aviex Technologies, Llc | Live bacterial vaccines resistant to carbon dioxide (CO2), acidic PH and/or osmolarity for viral infection prophylaxis or treatment |
KR101072708B1 (en) * | 2008-12-17 | 2011-10-11 | 씨제이제일제당 (주) | A corynebacteria having enhanced 5'-xanthosine monophosphate productivity and a method of producing 5'-xanthosine monophosphate using the same |
DE102013204728A1 (en) * | 2013-03-18 | 2014-09-18 | Wacker Chemie Ag | Microorganism strain and process for fermentative production of C4 compounds from C5 sugars |
US11180535B1 (en) | 2016-12-07 | 2021-11-23 | David Gordon Bermudes | Saccharide binding, tumor penetration, and cytotoxic antitumor chimeric peptides from therapeutic bacteria |
US11129906B1 (en) | 2016-12-07 | 2021-09-28 | David Gordon Bermudes | Chimeric protein toxins for expression by therapeutic bacteria |
KR101947945B1 (en) * | 2018-01-25 | 2019-02-13 | 씨제이제일제당 (주) | A microorganism of the genus Corynebacterium producing L-amino acids and method for producing L-amino acids using the same |
EP3594355A1 (en) | 2018-07-12 | 2020-01-15 | Evonik Operations GmbH | Method for the fermentative production of l-lysine |
CN109161555A (en) * | 2018-09-07 | 2019-01-08 | 大连大学 | A kind of marine low temperature malate dehydrogenase gene and its recombinant expression carrier |
KR102129379B1 (en) * | 2018-10-10 | 2020-07-02 | 한국과학기술원 | A recombinant microorganism into which a high activity malate dehydrogenase for producing succinic acid and a method for producing succinic acid using the same |
CN110129342B (en) * | 2019-05-17 | 2021-11-23 | 河南农业大学 | Molecular marker of corn high-lysine gene ZmcytMdh4 and application thereof |
KR102258159B1 (en) * | 2021-01-29 | 2021-05-27 | 씨제이제일제당 (주) | Novel malate dehydrogenase variant and a method for producing L-lysine using the same |
CN115449518B (en) * | 2021-06-08 | 2024-01-26 | 中国科学院天津工业生物技术研究所 | Polynucleotide with promoter activity based on mdh gene and application thereof |
JP2023038944A (en) * | 2021-09-07 | 2023-03-17 | 花王株式会社 | Method for producing aromatic compound |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
SK284235B6 (en) * | 1997-10-04 | 2004-11-03 | Forschungszentrum Juelich Gmbh | Method for microbial production of amino acids of the aspartate and/or glutamate family and agents which can be used in the said method |
CZ20014659A3 (en) * | 1999-06-25 | 2002-09-11 | Basf Aktiengesellschaft | Corynebacterium glutamicum genes encoding proteins involved in carbon metabolism and energy production |
WO2001000804A2 (en) * | 1999-06-25 | 2001-01-04 | Basf Aktiengesellschaft | Corynebacterium glutamicum genes encoding stress, resistance and tolerance proteins |
DE19929365A1 (en) | 1999-06-25 | 2000-12-28 | Basf Lynx Bioscience Ag | New genes from Corynebacterium glutamicum, useful for modifying cells for production of primary or secondary metabolites, e.g. amino or fatty acids |
JP2003144161A (en) * | 1999-07-02 | 2003-05-20 | Ajinomoto Co Inc | Method for producing l-amino acid |
JP4623825B2 (en) * | 1999-12-16 | 2011-02-02 | 協和発酵バイオ株式会社 | Novel polynucleotide |
-
2000
- 2000-07-04 DE DE10032350A patent/DE10032350A1/en not_active Withdrawn
-
2001
- 2001-06-20 AU AU2001279674A patent/AU2001279674A1/en not_active Abandoned
- 2001-06-20 WO PCT/EP2001/006928 patent/WO2002002778A1/en active IP Right Grant
- 2001-06-20 ES ES01957867T patent/ES2254459T3/en not_active Expired - Lifetime
- 2001-06-20 CN CN01812254A patent/CN1440458A/en active Pending
- 2001-06-20 AT AT01957867T patent/ATE312929T1/en not_active IP Right Cessation
- 2001-06-20 KR KR1020037000075A patent/KR100904744B1/en active IP Right Grant
- 2001-06-20 EP EP01957867A patent/EP1297148B1/en not_active Expired - Lifetime
- 2001-06-20 DE DE60115900T patent/DE60115900T2/en not_active Expired - Lifetime
- 2001-06-28 US US09/892,867 patent/US6995002B2/en not_active Expired - Fee Related
-
2003
- 2003-12-05 US US10/727,516 patent/US7067288B2/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
ATE312929T1 (en) | 2005-12-15 |
DE60115900T2 (en) | 2006-08-03 |
DE10032350A1 (en) | 2002-01-24 |
WO2002002778A8 (en) | 2002-02-07 |
EP1297148A1 (en) | 2003-04-02 |
US6995002B2 (en) | 2006-02-07 |
KR100904744B1 (en) | 2009-06-29 |
US7067288B2 (en) | 2006-06-27 |
ES2254459T3 (en) | 2006-06-16 |
KR20030013521A (en) | 2003-02-14 |
DE60115900D1 (en) | 2006-01-19 |
EP1297148B1 (en) | 2005-12-14 |
US20020037568A1 (en) | 2002-03-28 |
CN1440458A (en) | 2003-09-03 |
WO2002002778A1 (en) | 2002-01-10 |
US20040142454A1 (en) | 2004-07-22 |
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