WO2002010209A8 - Nucleotide sequences which code for the meth gene - Google Patents

Nucleotide sequences which code for the meth gene

Info

Publication number
WO2002010209A8
WO2002010209A8 PCT/EP2001/008220 EP0108220W WO0210209A8 WO 2002010209 A8 WO2002010209 A8 WO 2002010209A8 EP 0108220 W EP0108220 W EP 0108220W WO 0210209 A8 WO0210209 A8 WO 0210209A8
Authority
WO
WIPO (PCT)
Prior art keywords
polynucleotide
amino acid
acid sequence
code
nucleotide sequences
Prior art date
Application number
PCT/EP2001/008220
Other languages
French (fr)
Other versions
WO2002010209A1 (en
Inventor
Brigitte Bathe
Bettina Moeckel
Walter Pfefferle
Klaus Huthmacher
Christian Rueckert
Joern Kalinowski
Alfred Puehler
Michael Binder
Dieter Greissinger
Georg Thierbach
Original Assignee
Degussa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE10109687A external-priority patent/DE10109687A1/en
Application filed by Degussa filed Critical Degussa
Priority to EP01965135A priority Critical patent/EP1307475A1/en
Priority to AU2001285844A priority patent/AU2001285844A1/en
Publication of WO2002010209A1 publication Critical patent/WO2002010209A1/en
Publication of WO2002010209A8 publication Critical patent/WO2002010209A8/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1003—Transferases (2.) transferring one-carbon groups (2.1)
    • C12N9/1007—Methyltransferases (general) (2.1.1.)
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/142—Amino acids; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/12—Methionine; Cysteine; Cystine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/15—Corynebacterium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/185—Escherichia
    • C12R2001/19—Escherichia coli

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Polymers & Plastics (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Animal Husbandry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Food Science & Technology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The invention relates to an isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70 % to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70 % to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and processes for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the metH gene coding for 5 -methyltetrahydrofolate homocysteine methyltransferase (EC 2.1.1.13) is present in enhanced form, and the use of the polynucleotide sequences as hybridization probes.
PCT/EP2001/008220 2000-08-02 2001-07-17 Nucleotide sequences which code for the meth gene WO2002010209A1 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP01965135A EP1307475A1 (en) 2000-08-02 2001-07-17 Nucleotide sequences which code for the meth gene
AU2001285844A AU2001285844A1 (en) 2000-08-02 2001-07-17 Nucleotide sequences which code for the meth gene

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DE10038050 2000-08-02
DE10038050.6 2000-08-02
DE10109687.9 2001-02-28
DE10109687A DE10109687A1 (en) 2000-08-02 2001-02-28 New nucleotide sequences coding for the metH gene

Publications (2)

Publication Number Publication Date
WO2002010209A1 WO2002010209A1 (en) 2002-02-07
WO2002010209A8 true WO2002010209A8 (en) 2002-04-04

Family

ID=26006611

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2001/008220 WO2002010209A1 (en) 2000-08-02 2001-07-17 Nucleotide sequences which code for the meth gene

Country Status (3)

Country Link
EP (1) EP1307475A1 (en)
AU (1) AU2001285844A1 (en)
WO (1) WO2002010209A1 (en)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10217058A1 (en) 2002-04-17 2003-11-27 Basf Ag Process for the production of fine chemicals containing sulfur
DE10222858A1 (en) 2002-05-23 2003-12-04 Basf Ag Process for the fermentative production of sulfur-containing fine chemicals
DE10239082A1 (en) 2002-08-26 2004-03-04 Basf Ag Fermentative production of sulfur-containing fine chemicals, useful e.g. as feed additive, by culturing bacteria containing heterologous sequence for O-acetylhomoserine sulfhydrolase
DE10359668A1 (en) 2003-12-18 2005-07-14 Basf Ag Process for the preparation of methionine
DE102004035052A1 (en) 2004-07-20 2006-02-16 Basf Ag Microorganisms for the production of sulfur-containing compounds
US20090325244A1 (en) * 2006-10-24 2009-12-31 Basf Se Method of increasing gene expression using modified codon usage
EP2082045B1 (en) 2006-10-24 2015-03-18 Basf Se Method of reducing gene expression using modified codon usage
EP2097442A2 (en) * 2006-12-29 2009-09-09 Evonik Degussa GmbH Methionine synthases with reduced product inhibition
BRPI0807760A2 (en) 2007-02-19 2014-06-17 Evonik Degussa Gmbh CORINEFORM BACTERIA WITH FORMAT-THF SYNTETASE ACTIVITY AND / OR GLYCIN CLIVING ACTIVITY
AR086790A1 (en) 2011-06-29 2014-01-22 Metabolic Explorer Sa A MICROORGANISM FOR THE PRODUCTION OF METIONIN WITH IMPORTATION OF IMPROVED GLUCOSE
CN104411821B (en) 2012-06-18 2017-08-08 代谢探索者公司 Recombinant microorganism for fermenting and producing methionine
PL3039153T3 (en) 2013-08-30 2019-02-28 Evonik Degussa Gmbh Microorganism for methionine production with improved methionine synthase activity and methionine efflux
CA2959563A1 (en) 2014-09-01 2016-03-10 Metabolic Explorer Method and microorganism for methionine production by fermentation with improved methionine efflux
US20180223319A1 (en) 2015-08-07 2018-08-09 Evonik Degussa Gmbh Protein thiocarboxylate-dependent l-methionine production by fermentation
CN108291243A (en) 2015-11-27 2018-07-17 赢创德固赛有限公司 The method for producing l-methionine
EP3296404A1 (en) 2016-09-15 2018-03-21 Evonik Degussa GmbH Modified microorganism for production of methionine
JP2021521897A (en) 2018-05-04 2021-08-30 味の素株式会社 Method for producing L-methionine using Pantoea bacteria
WO2020067487A1 (en) 2018-09-28 2020-04-02 Ajinomoto Co., Inc. Method for producing l-methionine using a bacterium

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3908201A1 (en) * 1989-03-14 1990-09-27 Degussa METHOD FOR THE FERMENTATIVE MANUFACTURE OF L-LYSINE
KR920008381B1 (en) * 1990-12-31 1992-09-26 제일제당 주식회사 Method for producins l-lysine
EP0630406A1 (en) * 1992-02-20 1994-12-28 Genencor International, Inc. Biosynthesis of methionine using a reduced source of sulfur
DE19644567A1 (en) * 1996-10-26 1998-04-30 Forschungszentrum Juelich Gmbh Microbial production of substances from the aromatic metabolism / II
KR100878334B1 (en) * 1999-06-25 2009-01-14 백광산업 주식회사 Corynebacterium Glutamicum Genes Encoding Metabolic Pathway Proteins
JP4623825B2 (en) * 1999-12-16 2011-02-02 協和発酵バイオ株式会社 Novel polynucleotide

Also Published As

Publication number Publication date
AU2001285844A1 (en) 2002-02-13
EP1307475A1 (en) 2003-05-07
WO2002010209A1 (en) 2002-02-07

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